A cell culture kit

Abstract

The application relates to the technical field of kits, in particular to a cell culture kit which comprises a bearing box, the inner wall of the bearing box is provided with a lifting frame, the inner wall of the lifting frame is fixedly connected with a supporting plate, a plurality of reagent ports are formed in the upper side of the supporting plate, the upper side of the supporting plate is fixedly provided with tightening grooves located on the two sides of the reagent ports, the inner wall of the tightening grooves is slidably matched with a tightening block, and the upper side of the tightening block is fixedly connected with a pressure receiving plate. The tightening block, the pressure receiving plate, the arc-shaped ring, the extrusion spring, the top cover and the extrusion block are arranged, when the test tube is smaller than the reagent port, the extrusion spring extrudes the tightening block, the arc-shaped ring is pushed by the pressure receiving plate to clamp and temporarily fix the two ends of the test tube, then the top cover is closed, the extrusion block extrudes the pressure receiving plate, different test tubes can be fixed, and the universality and compatibility are improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of kits, in particular to a cell culture kit. BACKGROUND

[0002] Cell culture refers to a technology that simulates the in-vivo environment to make cells survive, grow, reproduce and maintain their main structure and function. Cell culture medium provides nutrients such as amino acids, vitamins, carbohydrates, etc. for cells, and is the material basis for cell growth and proliferation. Serum contains various growth factors, hormones and proteins, etc. Nutritional ingredients can promote cell adhesion, growth and differentiation. Antibiotics can prevent bacterial and fungal contamination and maintain the sterile state of the cell culture environment. During the culture process, a kit is often used to carry the vessel.

[0003] Through retrieval, Chinese patent application No. CN202220212991.X discloses an immune cell culture kit, relating to the technical field of cell culture kits. It comprises a box body and a box cover, the box cover is hinged to the box body through a hinge, a constant temperature plate is screw-connected in the box body, a reagent plate is screw-connected to the upper part of the constant temperature plate, a clamping mechanism is connected at the connection between the box body and the box cover, the clamping mechanism comprises a clamping block, the clamping block is screw-connected to the box cover, an installation slot is formed in the clamping block, one end of a first spring is fixedly connected to the inner wall of the installation slot, the other end of the first spring is fixedly connected to a clamping ball, a clamping slot is formed in the box body, a plug slot is connected through the sidewall of the box body in the middle of the clamping slot, a pressure rod is inserted through an L-shaped plate in the plug slot, and a limiting plate is connected to one side of the plug slot. When using the utility model, only the immune cells to be cultured need to be placed in the tube hole of the reagent plate in the kit, then the temperature inside the box body is adjusted, and the box cover is clamped with the box body using the clamping mechanism.

[0004] For the related technology in the above, the inventors found the following defects: when the cultured cells are placed in the reagent plate, when different cells are cultured, the diameters of the test tubes used will also be different. When the test tube is smaller than the tube hole, it will shake when placed inside, and even cause the test tube to break. At the same time, when different lengths of test tubes are carried, it cannot be applied to different lengths of culture tubes, which reduces the scope of application. UTILITY MODEL CONTENTS

[0005] In order to solve the problems mentioned in the above background art, the present application provides a cell culture kit.

[0006] The cell culture kit provided by the application adopts the technical scheme as follows: comprising a bearing box, an inner wall of the bearing box is provided with a lifting frame, an inner wall of the lifting frame is fixedly connected with a support plate, a plurality of reagent ports are formed in the upper side of the support plate, a plurality of tightening grooves are fixedly formed in the upper side of the support plate and located on both sides of the plurality of reagent ports, a tightening block is slidably connected with the inner wall of the tightening groove, a compression plate is fixedly connected with the upper side of the tightening block, and an arc-shaped ring is fixedly connected with one end of the plurality of compression plates.

[0007] An extrusion spring is fixedly connected between the plurality of tightening blocks and the tightening grooves, a top cover is rotatably connected with the upper side of the lifting frame, an extrusion block matched with the compression plate is fixedly connected with one side of the top cover, and an adjusting mechanism is arranged between the bearing box and the lifting frame.

[0008] Optionally, the adjusting mechanism comprises an extension block fixedly connected with one side of the lifting frame, a threaded hole formed in the upper side of the bearing box, and an adjusting screw rod rotatably connected with the lower side of the extension block and threadedly matched with the threaded hole.

[0009] Optionally, one side of each of the plurality of arc-shaped rings is fixedly connected with a soft pad, and a buffer sponge is fixedly connected with the lower side of the inner wall of the bearing box.

[0010] Optionally, one side of the lifting frame is fixedly connected with a clamping plate, and one side of the top cover is rotatably connected with a buckle matched with the clamping plate.

[0011] Optionally, a plurality of guide plates are fixedly connected with the upper side of the bearing box, and a plurality of hollow slot guide blocks slidably matched with the guide plates are fixedly connected with the lifting frame.

[0012] Optionally, an anti-slip elastic sheet is fixedly connected with one side of each of the extrusion blocks, and the anti-slip elastic sheet abuts against the compression plate.

[0013] Optionally, an operating ring is fixedly connected with the adjusting screw rod, and a plurality of anti-slip grooves are formed in the operating ring.

[0014] The application comprises the following beneficial technical effects:

[0015] The application comprises the following beneficial technical effects: BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 This is a schematic diagram of the overall structure in an embodiment of this application;

[0017] Figure 2 This is an embodiment of the present application. Figure 1 Enlarged structural diagram at point A;

[0018] Figure 3 This is a schematic diagram of the overall three-dimensional cross-section of the embodiments of this application;

[0019] Figure 4 This is an embodiment of the present application. Figure 3 Enlarged structural diagram in the middle;

[0020] Figure 5 This is a rear-view stereoscopic structural diagram of an embodiment of this application.

[0021] Reference numerals: 1. Carrier box; 2. Lifting frame; 3. Support plate; 4. Reagent port; 5. Tightening groove; 6. Tightening block; 7. Pressure plate; 8. Arc ring; 9. Compression spring; 10. Top cover; 11. Compression block; 12. Adjustment mechanism; 121. Extension block; 122. Threaded hole; 123. Adjusting screw; 13. Soft pad; 14. Buffer sponge; 15. Clamping plate; 16. Buckle; 17. Guide plate; 18. Empty groove guide block; 19. Anti-slip elastic sheet; 20. Operating ring; 21. Anti-slip groove. Detailed Implementation

[0022] The following is in conjunction with the appendix Figure 1 —5 provides further detailed description of this application.

[0023] This application discloses a cell culture kit. For example... Figures 1-5 As shown, a carrier box 1 is provided with a lifting frame 2 on its inner wall. A support plate 3 is fixedly connected to the inner wall of the lifting frame 2. Multiple reagent ports 4 are opened on the upper side of the support plate 3. Tightening grooves 5 are fixedly opened on both sides of the multiple reagent ports 4 on the upper side of the support plate 3. Tightening blocks 6 are slidably fitted on the inner wall of the tightening grooves 5. Pressure plates 7 are fixedly connected to the upper side of each tightening block 6. An arc-shaped ring 8 is fixedly connected to one end of each of the multiple pressure plates 7.

[0024] Please see Figure 5 Multiple guide plates 17 are fixedly connected to the upper side of the carrier box 1, and multiple slotted guide blocks 18 that slide with the guide plates 17 are fixedly connected to the lifting frame 2. Whenever the lifting frame 2 moves up and down inside the carrier box 1, the slotted guide blocks 18 slide on the outside of the guide plates 17 accordingly, which can protect multiple positions.

[0025] Please see Figure 1The side of the lifting frame 2 is fixedly connected with a clamping plate 15, and the side of the top cover 10 is rotatably connected with a clasp 16 matched with the clamping plate 15, which is clamped with the clamping plate 15 through the clasp 16, and the top cover 10 and the lifting frame 2 are fixed, and the upper side of the lifting frame 2 is sealed through the top cover 10.

[0026] Please refer to Figure 4 The side of each of the plurality of arc-shaped rings 8 is fixedly connected with a soft pad 13, and the lower side of the inner wall of the bearing box 1 is fixedly connected with a buffer sponge 14, which is used to protect the test tube between the arc-shaped ring 8 and the test tube after contact.

[0027] The plurality of tightening blocks 6 and the tightening grooves 5 are fixedly connected with extrusion springs 9, the upper side of the lifting frame 2 is rotatably connected with a top cover 10, the side of the top cover 10 is fixedly connected with extrusion blocks 11 matched with the pressure plates 7, and the bearing box 1 and the lifting frame 2 are provided with an adjusting mechanism 12.

[0028] Please refer to Figure 3 The adjusting mechanism 12 comprises an extension block 121 fixedly connected to the side of the lifting frame 2, a threaded hole 122 opened on the upper side of the bearing box 1, and an adjusting screw 123 rotatably connected to the lower side of the extension block 121 and threadedly connected with the threaded hole 122, when the test tube of different heights is adapted, the adjusting screw 123 is rotated in the threaded hole 122, so as to drive the lifting frame 2 to move upwards in the bearing box 1, thereby increasing the overall height, so as to be suitable for test tubes of different heights, and further improve the application range.

[0029] Please refer to Figure 3 The side of each of the extrusion blocks 11 is fixedly connected with an anti-skid elastic sheet 19, and the anti-skid elastic sheet 19 abuts against the pressure plate 7, when the extrusion block 11 extrudes the pressure plate 7, the anti-skid elastic sheet 19 can increase the friction between the pressure plate 7, thereby further fixing the pressure plate 7.

[0030] Please refer to Figure 2 The adjusting screw 123 is fixedly connected with an operating ring 20, and a plurality of anti-skid grooves 21 are opened on the operating ring 20, the adjusting screw 123 is operated by holding the operating ring 20, and the anti-skid grooves 21 facilitate increasing the friction when the operating ring 20 is operated.

[0031] The implementation principle of the cell culture kit embodiment of the present application is as follows: in use, the culture test tube can be inserted into the inside of the reagent port 4, when the test tube is smaller than the reagent port 4, the compression spring 9 can be squeezed to compress the compression block 6, the arc-shaped ring 8 can be pushed by the pressure plate 7 to clamp and temporarily fix the two ends of the test tube, the soft pad 13 can reduce the resistance between the test tubes, then the top cover 10 is closed, after the closing, the compression block 11 can compress the upper side of the pressure plate 7, so that the pressure plate 7 is fixed to a certain extent, so that the pressure plate 7 is fixed to different test tubes, improving the versatility and compatibility;

[0032] When the length of the test tube is high, the adjusting screw rod 123 can be rotated in the threaded hole 122 to drive the lifting frame 2 to move upwards in the inside of the bearing box 1, so as to improve the overall height, so as to be suitable for test tubes of different heights, and further improve the application range.

[0033] The above are preferred embodiments of the present application, which do not limit the protection scope of the present application, therefore: any equivalent changes made according to the structure, shape, principle of the present application should be covered within the protection scope of the present application.

Claims

1. A cell culture kit comprising a carrier cassette (1), characterized in that: The inner wall of the bearing box (1) is provided with a lifting frame (2), the inner wall of the lifting frame (2) is fixedly connected with a support plate (3), a plurality of reagent ports (4) are formed in the upper side of the support plate (3), the upper side of the support plate (3) is fixedly provided with a plurality of tightening grooves (5) located on both sides of the reagent ports (4), the inner wall of the tightening groove (5) is slidably connected with a tightening block (6), the upper side of the tightening block (6) is fixedly connected with a pressure receiving plate (7), one end of the plurality of pressure receiving plates (7) is fixedly connected with an arc-shaped ring (8); A plurality of tightening blocks (6) and tightening grooves (5) are fixedly connected with an extrusion spring (9), the upper side of the lifting frame (2) is rotatably connected with a top cover (10), one side of the top cover (10) is fixedly connected with an extrusion block (11) matched with the pressure receiving plate (7), and the bearing box (1) and the lifting frame (2) are provided with an adjusting mechanism (12).

2. The cell culture kit of claim 1, wherein: The adjusting mechanism (12) comprises an extension block (121) fixedly connected to one side of the lifting frame (2), a threaded hole (122) formed in the upper side of the bearing box (1), and an adjusting screw (123) rotatably connected to the lower side of the extension block (121) and threadedly connected with the threaded hole (122).

3. The cell culture kit of claim 1, wherein: One side of the plurality of arc-shaped rings (8) is fixedly connected with a soft pad (13), and the lower side of the inner wall of the bearing box (1) is fixedly connected with a buffer sponge (14).

4. The cell culture kit of claim 1, wherein: One side of the lifting frame (2) is fixedly connected with a clamping plate (15), and one side of the top cover (10) is rotatably connected with a clasp (16) matched with the clamping plate (15).

5. The cell culture kit of claim 1, wherein: The upper side of the bearing box (1) is fixedly connected with a plurality of guide plates (17), and the lifting frame (2) is fixedly connected with a plurality of hollow groove guide blocks (18) slidably connected with the guide plates (17).

6. The cell culture kit of claim 1, wherein: One side of the extrusion block (11) is fixedly connected with an anti-skid elastic sheet (19), and the anti-skid elastic sheet (19) abuts against the pressure receiving plate (7).

7. The cell culture kit of claim 2, wherein: The adjusting screw (123) is fixedly connected with an operating ring (20), and a plurality of anti-skid grooves (21) are formed in the operating ring (20).

Images