Cryopreservation box for cell culture

Abstract

The utility model provides a cryopreservation box for cell culture, and belongs to the technical field of cell culture equipment. Comprising a cryopreservation box main body, the multiple test tube racks are arranged, and the multiple test tube racks are independently arranged in the cryopreservation box main body; and the multiple inner cavities are formed in the multiple test tube racks correspondingly, the tops of the multiple inner cavities are flush, and the heights of the inner cavities are sequentially increased in a stepped mode in the direction from the two sides of the cryopreservation box body to the center of the cryopreservation box body. The test tube racks are independently arranged in the cryopreservation box main body, and are matched with the synergistic effect of the unique inner cavity structure, the supporting rods, the piston blocks and the springs in the cryopreservation box main body, so that the purpose that the test tube racks are arranged in an arch bridge shape after the box cover is opened is achieved; and the purpose of slowly and stably ascending the test tube rack is achieved by utilizing the matching of the structures such as the movable cover in the groove of the test tube rack, the air inlet formed in the movable cover and the air outlet communicated with the groove.

Description

TECHNICAL FIELD

[0001] The utility model relates to cell culture equipment technical field, especially a kind of cryopreservation box for cell culture. BACKGROUND

[0002] Cell culture has a crucial position in modern biological science research and clinical application. In order to ensure the activity and stability of cells during the culture process, proper preservation and processing of cells are needed. Cryopreservation box, as an important tool for cell preservation, is widely used in laboratories and biological research institutions. It can provide a stable low-temperature environment to ensure that cells are not damaged during long-term storage.

[0003] However, the traditional cryopreservation box is usually composed of a simple box body and a box cover, with shelves inside for placing test tubes to store test tubes containing cell samples. These cryopreservation boxes meet the basic needs of cell cryopreservation to some extent, but the test tube shelves in such cryopreservation boxes are mostly horizontally arranged, which has certain limitations in actual operation. When it is necessary to observe and take out the cell samples in the test tubes, the horizontally arranged test tube shelves make the operation more difficult. Due to the close arrangement of test tubes and limited viewing angle, researchers have difficulty in clearly observing the cell status in each test tube, and it is easy to bump into adjacent test tubes when taking out the test tubes, which may cause damage or confusion of samples. SUMMARY

[0004] The technical problem to be solved by the utility model is to provide a cryopreservation box for cell culture to solve the technical problem of inconvenient observation and taking out of cell samples in test tubes caused by horizontal arrangement of test tube shelves in traditional cryopreservation boxes, and possible impact and damage to cell samples in test tubes due to fast rising speed when the test tube shelves are rising.

[0005] Technical solution: To achieve the above object, the utility model discloses a kind of cryopreservation boxes for cell culture, comprising: cryopreservation box main body;Test tube rack, be equipped with multiple, and multiple test tube rack is independently arranged in cryopreservation box main body;Cavity, be equipped with multiple, and multiple cavity is respectively opened in multiple test tube rack, and multiple cavity top flush, the cavity height along cryopreservation box main body both sides center direction presents ladder shape gradually increases;Supporting rod, be equipped with multiple, multiple supporting rod with multiple cavity corresponds, and multiple supporting rod one end is connected with cryopreservation box main body, the other end is slidably connected with test tube rack;Piston block, be equipped with multiple, and multiple piston block with multiple supporting rod corresponds, piston block is connected on the other side of supporting rod, piston block is slidably connected with test tube rack, piston block is in cavity;Spring, be equipped with multiple, and multiple spring is arranged in multiple cavity, spring is in the side of piston block away from supporting rod, spring is used to push test tube rack up, and by the restriction of cavity height, promote multiple test tube rack after rising finally arch bridge shape, wherein the shape size of cryopreservation box main body should meet the standard specification of laboratory commonly used equipment, so as to be conveniently placed and used in conventional cell culture operating environment.The layout of its internal space should fully consider the size and movement range of test tube rack, ensure the cooperation precision and stability between various components, avoid friction or collision between components during long-term use, resulting in structural damage or functional failure.

[0006] In further embodiment, box cover, buckle on cryopreservation box main body, the box cover is used for the sealing of cryopreservation box main body, wherein the buckle mode between box cover and cryopreservation box main body should be designed to be simple and reliable, facilitate operator to open and close box cover quickly, simultaneously, in buckling state, it should have certain sealing stability, can withstand certain degree of external impact without loosening or leaking, and box cover can also be rotatably connected with cryopreservation box main body through hinge structure, so as to close and open cryopreservation box main body.

[0007] In further embodiment, groove, be equipped with multiple, and multiple groove is opened in multiple test tube rack, the opening number and position of groove correspond to the opening number and position of cavity;Air vent, be equipped with multiple, and multiple air vent corresponds with multiple groove, air vent is used for connecting groove and cavity, air vent is used when test tube rack goes down, promote that outside air is rapidly discharged in cavity, wherein the position of air vent should be selected at the junction of groove and cavity, and its internal passage should keep unobstructed, avoid affecting the movement of test tube rack due to poor exhaust.

[0008] In another embodiment, the movable cover is rotatably arranged in the groove, and the movable cover is used to close and open the exhaust port. The rotation shaft of the movable cover is firmly and flexibly arranged, and the sealing performance between the movable cover and the groove is good to prevent gas from leaking through the gap between the movable cover and the groove in the non-exhaust state.

[0009] In another embodiment, the air inlet is arranged on the movable cover, and the air inlet is used to facilitate the gas entering the inner cavity when the test tube rack moves. The position and direction of the air inlet are reasonably designed to ensure that the gas entering the inner cavity is uniformly distributed.

[0010] In another embodiment, the auxiliary rod is arranged in the groove, and the auxiliary rod is used to limit the rotation angle of the movable cover. The material of the auxiliary rod should have certain strength and toughness to withstand the friction and impact force generated during the rotation of the movable cover, and the surface of the auxiliary rod should be smooth to reduce the wear between the auxiliary rod and the movable cover. The installation position of the auxiliary rod should be reasonably adjusted according to the rotation range of the movable cover to ensure that the auxiliary rod can effectively limit the rotation of the movable cover during the entire movement of the movable cover.

[0011] In another embodiment, the air inlet has a small diameter to limit the rate of external air entering the inner cavity. The diameter of the air inlet should be accurately calculated and optimally designed according to the size of the test tube rack, the spring constant, and the required range of the rising speed control to ensure that the rising speed of the test tube rack can be effectively controlled stably under different use conditions. At the same time, the shape of the air inlet should be regular to avoid turbulence when the gas enters the inner cavity, which affects the rising stability of the test tube rack.

[0012] In another embodiment, the diameter of the exhaust port is greater than the diameter of the exhaust port, and the diameter of the exhaust port is the same as the diameter of the spring inner circle. The relationship between the diameter of the exhaust port and the diameter of the spring inner circle also considers the compactness and rationality of the structure to avoid affecting the installation and normal work of other components due to the excessive diameter of the exhaust port. When determining the diameter of the exhaust port, the descending speed requirement of the test tube rack, the volume of the inner cavity, and the compression and expansion characteristics of the spring should be fully considered to determine an optimal diameter of the exhaust port that can meet the rapid exhaust requirement and does not have negative effects on other components. At the same time, the edge of the exhaust port should be smoothly treated to reduce the resistance and noise when the air is discharged.

[0013] In another embodiment, the diameter of the groove is greater than the diameter of the movable cover. The difference between the diameter of the groove and the diameter of the movable cover should be reasonably determined according to the rotation amplitude of the movable cover, the size of the auxiliary rod, and the convenience of actual operation to avoid the structure being loose due to the excessive diameter of the groove or affecting the normal rotation of the movable cover.

[0014] In a further embodiment, the overall height of the test tube rack is matched with the inner cavity height of the cryopreservation box body, wherein the overall height tolerance of the test tube rack should be controlled within a very small range, matched with the inner cavity height tolerance of the cryopreservation box body, to ensure that the height adaptability of the two is always maintained at a high precision level under different production batches and use environments.

[0015] Beneficial effects: 1. Through the cooperation of the multiple test tube racks independently arranged in the cryopreservation box body, the unique inner cavity structure, the support rod, the piston block and the spring inside, the purpose of arranging the test tube racks in an arch bridge shape after opening the box cover is achieved; this arrangement greatly expands the operation space, allowing the user to clearly observe the state of the cell samples in each test tube from multiple angles, avoiding the visual obstruction caused by the traditional horizontal arrangement of the test tube racks; at the same time, when taking out or placing the test tubes, the fingers can more easily and accurately touch the target test tube, effectively reducing the risk of sample confusion or damage caused by accidental touching of other test tubes, achieving the significant effect of improving the convenience, accuracy and overall experimental efficiency of cell sample operation.

[0016] 2. When the box cover is opened and the spring pushes the test tube rack upwards, due to the small diameter of the air inlet, the rate of external air entering the inner cavity is limited, so that the test tube rack is subjected to stable air resistance during the upward movement, thereby achieving the purpose of slow and stable upward movement; this effectively avoids the impact force on the cell samples in the test tubes caused by the sudden and rapid upward movement of the test tube rack, fundamentally eliminates the possibility of damage to the cell samples caused by the speed factor, and achieves the effect of effectively protecting the integrity and activity of the cell samples during the opening operation of the cryopreservation box. BRIEF DESCRIPTION OF DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiment or prior art description. Obviously, the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained without creative labor.

[0018] Figure 1 The structure of the present application is shown in the figure.

[0019] Figure 2 The structure of the present application is shown in the figure. Figure 1 The structure of the present application is shown in the figure.

[0020] Figure 3 The structure of the present application is shown in the figure. Figure 1 The structure of the present application is shown in the figure.

[0021] Figure 4 The structure of the present application is shown in the figure.

[0022] Figure 5 is a structural schematic view of the inner cavity.

[0023] Figure 6 is Figure 2 a structural schematic view of A of

[0024] Figure 7 is Figure 2 a structural schematic view of B of

[0025] The reference signs in the drawings are as follows: 1, cryopreservation box body; 2, box cover; 3, test tube rack; 301, inner cavity; 302, groove; 303, exhaust port; 4, support rod; 401, piston block; 5, spring; 6, movable cover; 601, air inlet; 7, auxiliary rod. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical scheme and advantages of the utility model clearer, the technical scheme in the utility model is described clearly and completely. Obviously, the described embodiments are part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the protection scope of the utility model.

[0027] The application provides a cryopreservation box for cell culture, which solves the technical problem that the horizontal arrangement of the test tube rack in the conventional cryopreservation box causes inconvenience in observing and taking and placing the cell samples in the test tubes, and the cell samples in the test tubes can be impacted and damaged due to the high rising speed of the test tube rack. In actual use, the test tubes on the test tube rack can be conveniently observed and taken and placed, and the rising speed of the test tube rack is prevented from being too high, so that the cell samples in the test tubes are not impacted and damaged.

[0028] In order to better understand the above technical scheme, the above technical scheme will be described in detail in combination with the drawings of the specification and the specific embodiments.

[0029] Referring to Figures 1-7A cryopreservation box for cell culture includes: a cryopreservation box body 1; multiple test tube racks 3, each independently disposed within the cryopreservation box body 1; multiple inner cavities 301, each located within one of the test tube racks 3, with their tops flush and their height increasing in a stepped manner from both sides of the cryopreservation box body 1 towards the center; and multiple support rods 4, each corresponding to one of the inner cavities 301, with one end of each support rod connected to the cryopreservation box body 1 and the other end connected to one of the test tube racks 3. Sliding connection; multiple piston blocks 401 are provided, and the multiple piston blocks 401 correspond to the multiple support rods 4. The piston blocks 401 are connected to the other side of the support rods 4. The piston blocks 401 are slidably connected to the test tube rack 3. The piston blocks 401 are located in the inner cavity 301; multiple springs 5 ​​are provided, and the multiple springs 5 ​​are set in the multiple inner cavities 301. The springs 5 ​​are located on the side of the piston blocks 401 away from the support rods 4. The springs 5 ​​are used to push the test tube rack 3 upward, and through the height limitation of the inner cavity 301, the multiple test tube racks 3 are caused to eventually form an arch bridge shape after rising.

[0030] The special design of the inner cavity 301, combined with the support rod 4, piston block 401 and spring 5, enables the test tube rack 3 to be arranged in an arched shape when the lid 2 is open. This expands the operating space and solves the problem of inconvenience in observation and retrieval of traditional horizontally arranged test tube racks 3. It allows researchers to clearly observe cell samples in test tubes from various angles and to easily and accurately retrieve and place test tubes, improving the convenience and accuracy of operation and reducing the risk of sample damage due to improper operation.

[0031] The lid 2 is fastened onto the body 1 of the cryopreservation box, and the lid 2 is used to seal the body 1 of the cryopreservation box.

[0032] The lid 2 is snapped onto the cryopreservation box body 1, effectively sealing the cryopreservation box body 1, preventing external air, moisture and impurities from entering, maintaining a stable low temperature environment and sterile state inside the cryopreservation box, and ensuring the stability and activity of cell samples during long-term cryopreservation.

[0033] Multiple grooves 302 are provided, and the multiple grooves 302 are opened in multiple test tube racks 3. The number and position of the grooves 302 correspond to the number and position of the inner cavity 301. Multiple exhaust ports 303 are provided, and the multiple exhaust ports 303 correspond to the multiple grooves 302. The exhaust ports 303 are used to connect the grooves 302 and the inner cavity 301. The exhaust ports 303 are used to facilitate the rapid discharge of outside air into the inner cavity 301 when the test tube rack 3 moves downward.

[0034] The plurality of grooves 302 corresponding to the inner cavity 301 and the exhaust port 303, when the test tube rack 3 is descending, can quickly exhaust the air in the inner cavity 301, ensure that the test tube rack 3 can smoothly and quickly descend, avoid the problem of air pressure in the inner cavity 301 causing the test tube rack 3 to be blocked or unstable, and ensure the smoothness and efficiency of the operation of the cryopreservation box.

[0035] The movable cover 6 is rotatably arranged in the groove 302, and the movable cover 6 is used to close and open the exhaust port 303.

[0036] The movable cover 6 is rotatably arranged in the groove 302, and when the test tube rack 3 is ascending, the movable cover 6 can close the exhaust port 303.

[0037] The air inlet 601 is arranged on the movable cover 6, and the air inlet 601 is used to make gas enter the inner cavity 301 when the test tube rack 3 is ascending.

[0038] The air inlet 601 makes the gas slowly enter the inner cavity 301, provides a buffer for the test tube rack 3 to ascend, avoids the ascending speed being too fast, and thus protects the cell samples in the test tubes from being impacted.

[0039] The auxiliary rod 7 is arranged in the groove 302, and the auxiliary rod 7 is used to limit the rotation angle of the movable cover 6.

[0040] The auxiliary rod 7 is arranged in the groove 302, which can accurately limit the rotation angle of the movable cover 6, ensure that the movable cover 6 can be accurately positioned when opening and closing the exhaust port 303, and ensure that the opening and closing state of the exhaust port 303 is stable and reliable, and the exhaust port 303 cannot be closed due to excessive rotation.

[0041] The diameter of the air inlet 601 is small, which is used to limit the rate of external air entering the inner cavity 301.

[0042] The small diameter of the air inlet 601 can effectively limit the rate of external air entering the inner cavity 301, so as to accurately control the ascending speed of the test tube rack 3, avoid impacting and damaging the cell samples in the test tubes due to the too fast ascending speed, and ensure the safety and stability of the samples in the cell culture process.

[0043] The diameter of the exhaust port 303 is greater than the diameter of the exhaust port 303, and the diameter of the exhaust port 303 is the same as the inner diameter of the spring 5.

[0044] The diameter of the exhaust port 303 is greater than the diameter of the air inlet 601 and is the same as the inner diameter of the spring 5, which can quickly exhaust a large amount of air when the test tube rack 3 is descending, ensure that the test tube rack 3 can quickly and stably descend, and will not interfere with the operation of the spring 5.

[0045] The diameter of the groove 302 is greater than the diameter of the movable cover 6.

[0046] The groove 302 has a diameter larger than that of the movable cover 6, which provides sufficient space for the rotation of the movable cover 6 in the groove 302, so that the movable cover 6 can be freely and flexibly opened and closed to facilitate the restriction and adjustment of the rotation angle of the auxiliary rod 7.

[0047] The overall height of the test tube rack 3 is adapted to the height of the inner cavity 301 of the cryopreservation box body 1.

[0048] The overall height of the test tube rack 3 is adapted to the height of the inner cavity 301 of the cryopreservation box body 1, which ensures that the test tube rack 3 is fixed by the cooperation of the box cover 2 and the cryopreservation box body 1 when the box cover 2 is closed.

[0049] In use, when the cell culture cryopreservation box is needed, the box cover 2 is first opened, and as the box cover 2 is opened, the plurality of springs 5 in the compressed state begin to expand, pushing the piston block 401 connected thereto, which slides along the inner cavity 301 in the test tube rack 3. Due to the stepped increase in the height of the inner cavity 301 along the two sides of the cryopreservation box body 1 towards the center, the test tube rack 3 rises under the action of the springs 5 and finally presents an arch bridge arrangement; at this time, if the test tubes are to be taken out or put in, the researcher can conveniently operate the test tubes on the test tube rack 3 from various angles, because this arch bridge arrangement expands the operation space, avoiding the inconvenience of observation and taking and placing of the test tubes in the traditional horizontal arrangement of the test tube rack 3; when the box cover 2 is closed again, the box cover 2 exerts pressure on the test tube rack 3, causing the test tube rack 3 to begin to descend; during the descent of the test tube rack 3, the movable cover 6 in the groove 302 rotates to open the air outlet 303 (the air outlet 303 is used to communicate the groove 302 with the inner cavity 301) under the action of air pressure, and because the air outlet 303 has a large diameter (the maximum diameter of the air outlet 303 is the same as the inner diameter of the spring 5), it can promote the rapid exhaust of external air in the inner cavity 301, ensuring that the test tube rack 3 can smoothly and quickly descend to the reset position, and at the same time, the auxiliary rod 7 limits the rotation angle of the movable cover 6, ensuring the stability and accuracy of its movement; when the box cover 2 is opened again and the test tube rack 3 rises, external air can only slowly enter the inner cavity 301 through the small-diameter air inlet 601 formed in the movable cover 6, which limits the rate of external air entering the inner cavity 301, so that the rising speed of the test tube rack 3 is controlled, avoiding the impact and damage to the cell samples in the test tubes caused by the excessive rising speed, thereby achieving effective protection and convenient operation of the cell samples.

[0050] The figures expressed in the drawings are example figures, and the purpose is only to more intuitively show the key structures and connection relationships of the cryopreservation box for cell culture; in actual application, the appearance and size of the device can be adjusted and optimized according to specific needs.

[0051] The utility model covers any alternative, modification, equivalent method and scheme which are made on the essence and range of the utility model. In order to make the public have the thorough understanding of the utility model, the specific details are explained in the above preferred embodiment of the utility model, and the utility model can also be completely understood without the description of these details for the person skilled in the art. In addition, in order to avoid unnecessary confusion to the essence of the utility model, the well-known method, process, flow, element and circuit etc. are not explained in detail.

[0052] The above is only the preferred embodiment of the utility model, and it should be pointed out that, for ordinary skilled person in the art, under the premise of not departing from the principle of the utility model, a plurality of improvements and refinements can also be made, and these improvements and refinements should also be regarded as the protection range of the utility model.

Claims

1. A cryobox for cell culture, characterized in that, The application relates to a cryopreservation box. The cryopreservation box comprises a box body (1), a plurality of test tube racks (3) arranged in the box body (1), a plurality of cavities (301) arranged in the test tube racks (3), a plurality of supporting rods (4) corresponding to the cavities (301), a plurality of piston blocks (401) corresponding to the supporting rods (4), and a plurality of springs (5) arranged in the cavities (301). The cryopreservation box further comprises a box cover (2) arranged on the box body (1). The cryopreservation box further comprises a plurality of grooves (302) arranged in the test tube racks (3), a plurality of air outlet openings (303) corresponding to the grooves (302), a movable cover (6) arranged in the grooves (302), an air inlet opening (601) arranged on the movable cover (6), and an auxiliary rod (7) arranged in the grooves (302). The air inlet opening (601) has a small diameter, the air outlet opening (303) has a diameter larger than that of the air inlet opening (601), the diameter of the groove (302) is larger than that of the movable cover (6), the overall height of the test tube rack (3) is matched with the cavity height of the box body (1), and the spring (5) has the same inner diameter as the maximum diameter of the air outlet opening (303). The application also discloses a cryopreservation box. The application also discloses a cryopreservation box.

2. A cryobox for cell culture according to claim 1, characterized in that, The application also discloses a cryopreservation box. The application also discloses a cryopreservation box.

3. The cryobox for cell culture according to claim 1, wherein, The application also discloses a cryopreservation box. The application also discloses a cryopreservation box. The application also discloses a cryopreservation box.

4. A cryobox for cell culture according to claim 3, wherein, The application also discloses a cryopreservation box. The application also discloses a cryopreservation box.

5. A cryobox for cell culture according to claim 4, wherein, The application also discloses a cryopreservation box. The application also discloses a cryopreservation box.

6. A cryobox for cell culture according to claim 4, wherein, The application also discloses a cryopreservation box. The application also discloses a cryopreservation box.

7. A cryobox for cell culture according to claim 5, wherein: The application also discloses a cryopreservation box.

8. A cryobox for cell culture according to claim 5, wherein: The application also discloses a cryopreservation box.

9. A cryobox for cell culture according to claim 4, characterized in that: The application also discloses a cryopreservation box.

10. The cryobox for cell culture according to claim 1, wherein: The application also discloses a cryopreservation box. The application also discloses a cryopreservation box. The application also discloses a cryopreservation box. The application also discloses a cryopreservation box. The application also discloses a cryopreservation box. The application also discloses a cryopreservation box. The application also discloses a cryopreservation box. The application also discloses a cryopreservation box. The application also discloses a cryopreservation box. The application also discloses a cryopreservation box. The application also discloses a cryopreservation box. The application also discloses a cryopreservation box. The application also discloses a cryopreservation box. The application also discloses a cryopreservation box. The application also discloses a cryopreservation box. The application also discloses a cryopreservation box. The application also discloses a cryopreservation box. 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