Experimental culture device for anaerobic dehalogenation bacteria

By introducing a flipping and folding mechanism and a drive motor into the incubator, it is possible to observe bacterial growth without opening the door, thus solving the problem of temperature and humidity fluctuations during incubator observation and improving experimental stability and efficiency.

CN223607259UActive Publication Date: 2025-11-28QINGDAO UNIV
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Patent Information

Application Number
CN202423118209.6
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-12-17
Publication Date
2025-11-28
Estimated Expiration
2034-12-17

AI Technical Summary

Technical Problem

Existing incubators require frequent door openings when observing bacterial colonies, leading to fluctuations in temperature and humidity, disrupting the anaerobic environment, and affecting experimental stability.

Method used

An anaerobic dehalogenated bacteria experimental culture device was designed, which adopts a flipping and folding mechanism and a drive motor. The motor drives the rotating arm to flip the culture dish to the bottom of the observation glass plate, so that the bacterial growth can be observed without opening the door.

Benefits of technology

This reduces interference with the anaerobic environment and the risk of contamination, maintains experimental stability, and improves observation efficiency.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses an anaerobic dehalogenation bacterium experimental culture device which comprises a culture box, a plurality of fixed bases are fixedly arranged at the bottom of the inner wall of the culture box, and overturning and folding mechanisms are arranged at the tops of the fixed bases and comprise L-shaped connecting bases fixedly arranged in the middles of the two sides of the top ends of the fixed bases. According to the experimental culture device for anaerobic dehalogenation bacteria, when the first rotating arms and the second rotating arms are in a 90-degree state, the observation glass plate close to the top of the culture box is arranged, so that the first rotating arms and the second rotating arms are in a 90-degree state, and the first rotating arms and the second rotating arms are in a 90-degree state, so that the first rotating arms and the second rotating arms are in a 90-degree state, and the observation glass plate is close to the top of the culture box; a researcher can directly check the growth condition of thalli in the culture dish without opening a door of the culture box, so that the interference and pollution risks of an experimental environment are reduced.
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Description

TECHNICAL FIELD

[0001] The utility model relates to an experimental culture device, in particular to an anaerobic dehalogenating bacteria experimental culture device. BACKGROUND

[0002] The anaerobic dehalogenating bacteria experimental culture device is a culture equipment for providing an anaerobic environment for anaerobic dehalogenating bacteria.

[0003] The common culture box has limited observation angle of bacterial colonies, and researchers need to frequently open the door to check the culture condition, which will cause the fluctuation of temperature and humidity in the box, destroy the anaerobic environment and affect the stability of the experiment.

[0004] It should be noted that the above content belongs to the technical cognition of the inventor and does not necessarily constitute the prior art. UTILITY MODEL CONTENT

[0005] To solve the above problems, the utility model aims at providing an anaerobic dehalogenating bacteria experimental culture device,

[0006] To achieve the above purpose, the utility model provides an anaerobic dehalogenating bacteria experimental culture device, which comprises a culture box.

[0007] A plurality of fixed bases are fixedly arranged at the bottom of the inner wall of the culture box, and a turnover folding mechanism is arranged at the top of each fixed base.

[0008] The turnover folding mechanism comprises an L-shaped connecting seat fixedly arranged at the middle position of the top of the fixed base, first rotating arms are rotatably arranged at the inner walls of the two L-shaped connecting seats, a connecting rod is fixedly arranged between the two first rotating arms, first hinge seats are fixedly arranged at one end of the two first rotating arms, the two first hinge seats are rotatably connected with the two sides of the corresponding U-shaped clamping seat, and the two U-shaped clamping seats are fixedly arranged at one end of the corresponding second rotating arms.

[0009] In one example, one side of one of the L-shaped connecting seats is provided with a first connecting lug at the top, one of the U-shaped clamping seats is provided with a second connecting lug at the top, one end of one side of the other L-shaped connecting seat is provided with a third connecting lug, and the top of the other U-shaped clamping seat is provided with a fourth connecting lug; one side of the first connecting lug and one side of the third connecting lug are rotationally connected to one end of the corresponding supporting rotating rod, and the other end of the two supporting rotating rods is rotationally connected to one end of the second connecting lug and the fourth connecting lug, respectively.

[0010] In one example, the inner sides of the two second rotating arms are fixedly connected to the two sides of the first cross frame, and the top of the first cross frame is provided with a plurality of culture dishes.

[0011] In one example, a second cross frame is fixedly arranged between the two first rotating arms, and the bottom of the second cross frame is provided with a buffer pad.

[0012] In one example, one side of the incubator is hingedly provided with a sealed box door.

[0013] In one example, the top of the incubator is provided with a groove, and the inside of the groove is fixedly provided with an observation glass plate.

[0014] The anaerobic dehalogenating bacteria experimental culture device can bring the following beneficial effects:

[0015] The incubator, the sealed box door, the fixed base, the L-shaped connecting seat, the first cross frame, the second cross frame, the second rotating arm, the driving motor, the first connecting lug, the fourth connecting lug, the third connecting lug, the supporting rotating rod, the first rotating arm, the connecting rod, the U-shaped clamping seat, the hinged seat, the culture dish and the buffer pad are used, the driving motor drives the first rotating arm to rotate, the first rotating arm is rotationally connected to the second rotating arm, and the supporting rotating rod is rotationally arranged between the corresponding connecting lugs of the first rotating arm and the second rotating arm, so that the first cross frame between the two first rotating arms can always maintain a parallel state with the fixed base during the rotation of the first rotating arm, and when the first rotating arm and the second rotating arm are in a 90-degree state, the observation glass plate close to the top of the incubator allows the researchers to directly observe the growth of the bacteria in the culture dish without opening the incubator door, thereby reducing the interference and pollution risk of the experimental environment. BRIEF DESCRIPTION OF DRAWINGS

[0016] The accompanying drawings, which are included to provide a further understanding of the present application and constitute a part of this application, illustrate certain illustrative embodiments of the present application and together with the description serve to explain the present application. In the drawings:

[0017] Figure 1 The accompanying drawings, which are included to provide a further understanding of the present application and constitute a part of this application, illustrate certain illustrative embodiments of the present application and together with the description serve to explain the present application. In the drawings:

[0018] Figure 2 It is the structure schematic view of the turnover folding mechanism of the utility model;

[0019] Figure 3 It is the structure schematic view of the first rotating arm and the second rotating arm of the utility model;

[0020] Figure 4 It is the utility model Figure 2 The enlarged view at A;

[0021] Figure 5 It is the utility model Figure 2 The enlarged view at B;

[0022] Figure 6 It is the structure schematic view of the first horizontal frame of the utility model;

[0023] Figure 7 It is the structure schematic view of the second horizontal frame of the utility model.

[0024] In the figure: 1, incubator; 2, sealed door; 3, fixed base; 4, slot; 5, observation glass plate; 6, L-shaped connecting base; 7, first horizontal frame; 8, second horizontal frame; 9, second rotating arm; 10, drive motor; 11, first connecting lug; 12, fourth connecting lug; 13, third connecting lug; 14, supporting rotating rod; 15, first rotating arm; 16, connecting rod; 17, U-shaped clamping base; 18, hinged base; 19, culture dish; 20, buffer pad; 21, second connecting lug. Specific implementation

[0025] In order to more clearly illustrate the overall concept of the utility model, the following will be described in detail with the help of the attached drawings of the specification in an exemplary manner.

[0026] As Figures 1-7 Indicated, the embodiment of the utility model proposes an anaerobic dehalogenating bacteria experimental culture device, including incubator 1:

[0027] The bottom of the inner wall of the incubator 1 is fixed with a plurality of fixed bases 3, and the top of the plurality of fixed bases 3 is provided with a turnover folding mechanism:

[0028] The turnover folding mechanism includes an L-shaped connecting base 6 fixedly arranged at the middle position of the top end of the fixed base 3, the inner wall of the two L-shaped connecting bases 6 is rotatably provided with a first rotating arm 15, a connecting rod 16 is fixedly arranged between the two first rotating arms 15, the one end of the two first rotating arms 15 is fixedly provided with a hinged base 18, the two hinged bases 18 are rotatably connected with the two sides of the corresponding U-shaped clamping base 17, and the two U-shaped clamping bases 17 are fixedly arranged at one end of the corresponding second rotating arm 9. The middle position of the outer side of one of the L-shaped connecting bases 6 is fixedly provided with a drive motor 10, and the output end of the drive motor 10 is fixedly connected with one end of one side of one of the first rotating arms 15.

[0029] Specifically, one side of the top of one of the L-shaped connecting seats 6 is fixedly provided with a first connecting lug 11, the top end of one of the U-shaped clamping seats 17 is fixedly provided with a second connecting lug 21, one end of one side of the other L-shaped connecting seat 6 is fixedly provided with a third connecting lug 13, the top end of the other U-shaped clamping seat 17 is fixedly provided with a fourth connecting lug 12, one side of the first connecting lug 11 and one side of the third connecting lug 13 are rotationally connected with one end of the corresponding support rotating rod 14, and the other end of the two support rotating rods 14 is respectively rotationally connected with one end of the second connecting lug 21 and the fourth connecting lug 12.

[0030] Specifically, the inner sides of the two second rotating arms 9 are fixedly connected with the two sides of the first cross frame 7, and the top end of the first cross frame 7 is fixedly provided with a plurality of culture dishes 19.

[0031] Specifically, a second cross frame 8 is fixedly arranged between the two first rotating arms 15, and a buffer pad 20 is fixedly arranged at the bottom end of the second cross frame 8.

[0032] Specifically, one side of the incubator 1 is hingedly provided with a sealing box door 2.

[0033] Specifically, a groove 4 is formed at the top end of the incubator 1, and an observation glass plate 5 is fixedly arranged in the groove 4.

[0034] Working principle: adjust and install the multiple fixed bases 3 at the bottom of the inner wall of the incubator 1 in place, ensure that each fixed base 3 is stably connected at the bottom of the inner wall of the incubator 1, install the multiple culture dishes 19 on the first cross frame 7, place culture medium and dehalogenating bacteria in the culture dishes 19 according to experimental requirements, close and seal the sealing box door 2 of the incubator 1, and ensure that the inside of the device maintains good sealing performance, so as to maintain an anaerobic environment in the subsequent process.

[0035] The driving motor 10 is started, the output end of the driving motor 10 is fixedly connected with one end of the first rotating arm 15, the first rotating arm 15 is driven to rotate through the output rotary power, when the driving motor 10 drives the first rotating arm 15 to rotate, because the first rotating arm 15 and the second rotating arm 9 are rotationally connected through the U-shaped clamping seat 17 and the hinged seat 18, the second rotating arm 9 starts to rotate synchronously under the driving, at the same time, because the support rotating rod 14 is rotationally arranged between the corresponding first connecting lug 11 and the second connecting lug 21 of the first rotating arm 15 and the second rotating arm 9 and between the third connecting lug 13 and the fourth connecting lug 12, the culture dish 19 on the first horizontal frame 7 can keep parallel with the fixed base 3, with the continuous rotation of the first rotating arm 15, the first horizontal frame 7 is gradually turned over to reach the position of 90 degrees, so that the culture dish 19 is close to the observation glass plate 5 on the top of the incubator 1, the first horizontal frame 7 continues to turn over 90 degrees, at this time, the second horizontal frame 8 is turned over 180 degrees, the buffer pad 20 is at the bottom of the second horizontal frame 8 from the initial state, and is turned over until the buffer pad 20 is above, at this time, the first horizontal frame 7 is above the second horizontal frame 8, when all the observation and culture periods are finished, the driving motor 10 is turned off, the turning over operation of the culture dish 19 is stopped, the sealed box door 2 can be opened by the experimental personnel, the culture dish 19 and the experimental sample are taken out, and subsequent analysis and processing are carried out.

[0036] Each embodiment in the specification is described in a progressive manner, and the same and similar parts between each embodiment can be referred to each other, and each embodiment mainly explains the difference from other embodiments. Especially, for the system embodiment, because it is basically similar to the method embodiment, the description is relatively simple, and the related parts can be referred to the part of the method embodiment.

[0037] The above only describes the embodiments of the present application and is not used to limit the present application. The present application can be changed and varied by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the scope of the claims of the present application.

Claims

1. An anaerobic dehalogenating bacteria experimental culture device, comprising a culture box (1): characterized in that A plurality of fixed bases (3) are fixedly arranged at the bottom of the inner wall of the culture box (1), and the top of each fixed base (3) is provided with a turnover folding mechanism: The turnover folding mechanism comprises L-shaped connecting seats (6) fixedly arranged at the middle positions of the top ends of the fixed bases (3), the inner walls of the two L-shaped connecting seats (6) are rotatably provided with first rotating arms (15), a connecting rod (16) is fixedly arranged between the two first rotating arms (15), the one ends of the two first rotating arms (15) are fixedly provided with hinged seats (18), the two hinged seats (18) are rotatably connected with the two sides of corresponding U-shaped clamping seats (17), the two U-shaped clamping seats (17) are fixedly arranged at the one ends of corresponding second rotating arms (9), one of the L-shaped connecting seats (6) is fixedly arranged with a drive motor (10) at the middle position of the outer side, and the output end of the drive motor (10) is fixedly connected with one end of one side of the first rotating arm (15).

2. The experimental culture device for anaerobic dehalogenating bacteria according to claim 1, characterized in that: The top of one side of one of the L-shaped connecting seats (6) is fixedly provided with a first connecting lug (11), the top end of one of the U-shaped clamping seats (17) is fixedly provided with a second connecting lug (21), one end of one side of the other L-shaped connecting seat (6) is fixedly provided with a third connecting lug (13), the top end of the other U-shaped clamping seat (17) is fixedly provided with a fourth connecting lug (12), one side of the first connecting lug (11) and one side of the third connecting lug (13) are rotatably connected with one end of a corresponding supporting rotating rod (14), and the other ends of the two supporting rotating rods (14) are rotatably connected with one end of the second connecting lug (21) and one end of the fourth connecting lug (12) respectively.

3. The experimental culture device for anaerobic dehalogenating bacteria according to claim 1, wherein: The inner sides of the two second rotating arms (9) are fixedly connected with the two sides of a first cross frame (7), and the top end of the first cross frame (7) is fixedly provided with a plurality of culture dishes (19).

4. The experimental culture device for anaerobic dehalogenating bacteria according to claim 1, characterized in that: A second cross frame (8) is fixedly arranged between the two first rotating arms (15), and a buffer pad (20) is fixedly arranged at the bottom end of the second cross frame (8).

5. The experimental culture device for anaerobic dehalogenating bacteria according to claim 1, wherein: A sealing box door (2) is hingedly arranged at one side of the culture box (1).

6. The experimental culture device for anaerobic dehalogenating bacteria according to claim 1, wherein: A groove (4) is formed at the top end of the culture box (1), and an observation glass plate (5) is fixedly arranged in the groove (4).