In-situ hybridization instrument

Abstract

The utility model relates to the technical field of hybridization instruments, in particular to an in-situ hybridization instrument which comprises a machine body, a controller is fixedly connected to one side of the machine body, a top cover is rotatably connected to the top of the machine body, a plurality of slides are placed on the inner side of the machine body, and a limiting plate is inserted into the inner side of the machine body. The top of the limiting plate is fixedly connected with a humidifying box, the inner side of the machine body is fixedly connected with a connecting pipe, one side of the connecting pipe is slidably connected with a piston rod, and one side of the piston rod is in threaded connection with a threaded rod. The glass slide humidifying device has the advantages that the glass slide is fixed on the inner side of the machine body through suction force generated by the piston rod and the connecting pipe and dead weight of the clamping groove, the limiting plate and the humidifying box, the influence of vibration on the glass slide is prevented, and generated condensed water drops are drained back into the water storage tank through the heating plate, the arc-shaped cover plate, the flow guide groove and the water tank at the top of the machine body. Sufficient water in the device is ensured, and the accuracy of experimental results is prevented from being influenced by water loss of hybridization liquid.

Description

TECHNICAL FIELD

[0001] The utility model relates to hybridization instrument technical field, especially in situ hybridization instrument. BACKGROUND

[0002] When detecting part of pathogenic microorganism nucleic acid, the sample to be detected needs to be attached to the detection object, so that the detection object is in full contact with the corresponding detection solution, so that the sample to be detected reacts in the corresponding detection solution, and people make corresponding evaluation on the virus according to the detection result, wherein the in situ hybridization experiment is basically carried out through the in situ hybridization instrument, the in situ hybridization instrument can automatically complete the denaturation and hybridization process under strict temperature control and suitable humidity environment according to the setting of the experimenter, the existing hybridization instrument causes the salt concentration to increase due to the evaporation of water during long-time work, and finally may cause the experiment to fail.

[0003] To this end, the utility model provides an in situ hybridization instrument to solve the problem. UTILITY MODEL CONTENT

[0004] The utility model aims at solving at least one of the technical defects.

[0005] Therefore, one purpose of the utility model is to provide an in situ hybridization instrument to solve the problems mentioned in the background art and overcome the deficiencies in the prior art.

[0006] In order to achieve the above-mentioned purpose, an embodiment of the utility model provides an in situ hybridization instrument, which comprises a body, a controller is fixedly connected to one side of the body, a top cover is rotatably connected to the top of the body, a plurality of slides are placed in the inside of the body, a limiting plate is inserted into the inside of the body, a humidifying box is fixedly connected to the top of the limiting plate, a connecting pipe is fixedly connected to the inside of the body, a piston rod is slidably connected to one side of the connecting pipe, and a threaded rod is threadedly connected to one side of the piston rod.

[0007] It is preferred that the inside of the top cover is fixedly connected with an arc-shaped cover plate, and one side of the arc-shaped cover plate is fixedly connected with a heating plate.

[0008] It is preferred that the humidifying box comprises a water storage groove and a flow guide groove.

[0009] The above-mentioned scheme achieves the technical effect of ensuring the humidity inside the device through the humidifying box.

[0010] It is preferred that the top of the body is provided with a plurality of clamping grooves, the inner surface of the clamping grooves is matched with the outer surface of the slides, and the slides are placed in the clamping grooves.

[0011] Preferably, one side of the limiting plate is provided with a first through groove, an inner surface of the first through groove is matched with an outer surface of the glass sheet, and one side of the glass sheet is inserted into the first through groove.

[0012] The technical effect achieved by the above scheme is that the glass sheet is pressed and fixed in the machine body by the self-weight of the limiting plate and the humidifying box.

[0013] Preferably, one side of the machine body is provided with a second recess, an inner surface of the second recess is matched with an outer surface of the connecting pipe, and the connecting pipe is fixedly connected to the inside of the second recess.

[0014] Preferably, one side of the machine body is provided with a first through hole, an inner surface of the first through hole is matched with an outer surface of one side of the piston rod, and one side of the piston rod is slidingly connected to the inside of the first through hole.

[0015] Preferably, one side of the piston rod is provided with a second through hole, an inner surface of the second through hole is matched with an outer surface of one side of the threaded rod, and the threaded rod is threadedly connected to the inside of the second through hole.

[0016] The technical effect achieved by the above scheme is that the threaded rod drives the outward movement of the piston rod, so that the suction force generated by the connecting pipe fixes the glass sheet in the machine body.

[0017] Preferably, one side of the machine body is provided with a water tank, a width of the water tank is greater than a width of one side of the arc-shaped cover plate, and one side of the arc-shaped cover plate is inserted into the inside of the water tank.

[0018] Preferably, an inner width of the flow guide groove is greater than a width of one side of the water tank, and the flow guide groove is spliced with one side of the water tank.

[0019] The technical effect achieved by the above scheme is that the condensed water droplets are guided back into the water storage tank through the arc-shaped cover plate, the water tank and the flow guide groove.

[0020] Compared with the prior art, the utility model has the advantages and beneficial effects that:

[0021] The glass sheet is fixed inside the machine body by the suction force generated by the piston rod and the connecting pipe, the clamping groove, the self-weight of the limiting plate and the humidifying box, the influence of vibration on the glass sheet is prevented, then the condensed water droplets are guided back into the water storage tank through the heating plate, the arc-shaped cover plate, the flow guide groove and the water tank at the top of the machine body, the water in the device is ensured to be sufficient, and the influence of the water loss of the hybridization solution on the experimental results is avoided.

[0022] The additional aspects and advantages of the utility model will be partially given in the following description, some will become obvious from the following description, or be understood through the practice of the utility model. BRIEF DESCRIPTION OF DRAWINGS

[0023] The above and / or additional aspects and advantages of the utility model will become apparent and more readily appreciated from the following description of the embodiments, with reference to the following drawings, in which:

[0024] Figure 1 It is whole structure schematic diagram according to the embodiment of the utility model;

[0025] Figure 2 It is machine body internal structure schematic diagram according to the embodiment of the utility model;

[0026] Figure 3 It is machine body internal structure cross section schematic diagram according to the embodiment of the utility model;

[0027] Figure 4 It is top cover internal structure schematic diagram according to the embodiment of the utility model;

[0028] Figure 5 It is connecting pipe connecting structure schematic diagram according to the embodiment of the utility model.

[0029] In the drawing: 1-machine body, 2-controller, 3-top cover, 4-glass, 5-limiting plate, 6-humidification box, 7-connecting pipe, 8-piston rod, 9-threaded rod, 10-water tank, 301-arc cover plate, 302-heating plate, 601-water storage tank, 602-flow guide groove. DETAILED DESCRIPTION

[0030] As Figures 1 to 5 Shown in the figure, a kind of in situ hybridization instrument, it includes machine body 1, the side of machine body 1 is fixedly connected with controller 2, controller 2 is electrically connected with heating plate 302, the top of machine body 1 is rotatably connected with top cover 3, heating plate 302 is composed of multiple heating wires and is attached on the upside of arc cover plate 301, the inside of machine body 1 is placed with several glass 4, the outlet of connecting pipe 7 side is set in the clamping groove where glass 4 is placed, the inside of machine body 1 is inserted with limiting plate 5, the top of machine body 1 is provided with recess that is compatible with limiting plate 5, limiting plate 5 is inserted in recess interior, and the clamping groove where glass 4 is placed is also located in recess interior, the inside width of arc cover plate 301 is greater than the width of limiting plate 5, the top of limiting plate 5 is fixedly connected with humidification box 6, the inside of machine body 1 is fixedly connected with connecting pipe 7, the side of connecting pipe 7 is slidably connected with piston rod 8, the side of piston rod 8 is threadedly connected with threaded rod 9, the one end of threaded rod 9 is rotatably connected to the side wall of machine body 1 after passing through the side of piston rod 8.

[0031] The inner side of the top cover 3 is fixedly connected with an arc-shaped cover plate 301, and one side of the arc-shaped cover plate 301 is fixedly connected with a heating plate 302.

[0032] The humidifying box 6 comprises a water storage groove 601 and a flow guide groove 602.

[0033] The top of the machine body 1 is provided with a plurality of clamping grooves, the inner surface of the clamping grooves is matched with the outer surface of the glass sheet 4, the glass sheet 4 is placed in the clamping grooves, and in use, the glass sheet 4 is placed in the clamping grooves on the top of the machine body 1, and the limiting plate 5 is placed in the recess on the top of the machine body 1, so that the glass sheet is pressed in the machine body 1 by the self-weight of the water in the humidifying box 6 and the limiting plate 5.

[0034] One side of the limiting plate 5 is provided with a first through groove, the inner surface of the first through groove is matched with the outer surface of the glass sheet 4, and one side of the glass sheet 4 is inserted into the first through groove.

[0035] One side of the machine body 1 is provided with a second recess, the inner surface of the second recess is matched with the outer surface of the connecting pipe 7, the connecting pipe 7 is fixedly connected in the second recess, then the threaded rod 9 is rotated to drive the piston rod 8 to move outward, the air pressure in the connecting pipe 7 is reduced, so that a certain suction force is generated on the glass sheet 4 in the clamping groove through the through hole, and the glass sheet 4 is fixed, thereby preventing the glass sheet from being affected due to vibration.

[0036] One side of the machine body 1 is provided with a first through hole, the inner surface of the first through hole is matched with the outer surface of one side of the piston rod 8, one side of the piston rod 8 is slidably connected in the first through hole, the top cover 3 is closed, the hybridization instrument is adjusted, the heating temperature of the heating plate 302 on the inner side of the top cover 3 is adjusted through the controller 2, and since the salt concentration of the ultrapure water filled in the humidifying box 6 is lower than that of the hybridization solution, the evaporation is faster.

[0037] One side of the piston rod 8 is provided with a second through hole, the inner surface of the second through hole is matched with the outer surface of one side of the threaded rod 9, and the threaded rod 9 is threadedly connected in the second through hole.

[0038] One side of the machine body 1 is provided with a water groove 10, the width of the water groove 10 is greater than that of one side of the arc-shaped cover plate 301, one side of the arc-shaped cover plate 301 is inserted into the water groove 10, the water vapor in the saturated space of the evaporated pure water, and the loss of the liquid on the glass sheet 4 is well prevented, and the evaporated water in the internal space will quickly reach the saturated water vapor.

[0039] The inner side of the flow guide groove 602 is wider than one side of the water groove 10, the flow guide groove 602 is spliced with one side of the water groove 10, and the arc-shaped cover plate 301 on the inner side of the top cover 3 guides the condensed water drops to the water groove 10 on the inner top of the machine body 1, and then flows back to the water storage groove 601 through the flow guide groove 602, so as to ensure the sufficiency of the water in the device and avoid the influence of the water loss of the hybridization solution on the experimental results.

[0040] Compared with the prior art, the utility model has the following beneficial effects relative to the prior art:

[0041] The slide 4 is fixed in the inside of the machine body 1 by the suction force generated by the piston rod 8 and the connecting pipe 7, the clamping groove, the limiting plate 5 and the self weight of the humidifying box 6, the influence of vibration on the slide 4 is prevented, then the generated condensed water drops are guided back to the water storage tank 601 through the heating plate 302, the arc-shaped cover plate 301, the flow guide groove 602 and the water tank 10 at the top of the machine body 1, the sufficiency of the moisture inside the device is ensured, and the influence of the water loss of the hybridization solution on the experimental result is avoided.

Claims

1. An in situ hybridization apparatus, characterized by: Including the body (1), one side of the body (1) is fixedly connected with the controller (2), the top of the body (1) is rotatably connected with the top cover (3), the inner side of the body (1) is placed with several glass slides (4), the inner side of the body (1) is inserted with the limiting plate (5), the top of the limiting plate (5) is fixedly connected with the humidifying box (6), the inner side of the body (1) is fixedly connected with the connecting pipe (7), one side of the connecting pipe (7) is slidably connected with the piston rod (8), one side of the piston rod (8) is threadedly connected with the threaded rod (9).

2. An in situ hybridization apparatus according to claim 1, wherein: The inner side of the top cover (3) is fixedly connected with the arc-shaped cover plate (301), one side of the arc-shaped cover plate (301) is fixedly connected with the heating plate (302).

3. An in situ hybridization apparatus according to claim 2, wherein: The humidifying box (6) comprises a water storage tank (601) and a flow guide groove (602).

4. An in situ hybridization apparatus according to claim 3, wherein: The top of the body (1) is provided with a plurality of clamping grooves, the inner surface of the clamping groove is matched with the outer surface of the glass slide (4), and the glass slide (4) is placed in the clamping groove.

5. An in situ hybridization apparatus according to claim 4, wherein: One side of the limiting plate (5) is provided with a first through groove, the inner surface of the first through groove is matched with the outer surface of the glass slide (4), and one side of the glass slide (4) is inserted into the first through groove.

6. An in situ hybridization apparatus according to claim 5, wherein: One side of the body (1) is provided with a second recess, the inner surface of the second recess is matched with the outer surface of the connecting pipe (7), and the connecting pipe (7) is fixedly connected in the second recess.

7. An in situ hybridization apparatus according to claim 6, wherein: One side of the body (1) is provided with a first through hole, the inner surface of the first through hole is matched with the outer surface of one side of the piston rod (8), and one side of the piston rod (8) is slidably connected in the first through hole.

8. An in situ hybridization apparatus according to claim 7, wherein: One side of the piston rod (8) is provided with a second through hole, the inner surface of the second through hole is matched with the outer surface of one side of the threaded rod (9), and the threaded rod (9) is threadedly connected in the second through hole.

9. An in situ hybridization apparatus according to claim 8, wherein: One side of the body (1) is provided with a water tank (10), the width of the water tank (10) is greater than the width of one side of the arc-shaped cover plate (301), and one side of the arc-shaped cover plate (301) is inserted into the water tank (10).

10. An in situ hybridization apparatus according to claim 9, wherein: The inner side of the flow guide groove (602) is wider than the width of one side of the water tank (10), and the flow guide groove (602) is connected with one side of the water tank (10).

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