Visual system tank for automatic culture of stem cells

Abstract

The utility model belongs to the technical field of stem cell culture, particularly relates to a visual system tank for automatic culture of stem cells, and provides the following scheme aiming at the problems that in the using process of an existing system tank, gas with specific composition and a culture solution are inconvenient to uniformly mix, and the culture solution is inconvenient to uniformly heat: the visual system tank comprises a culture tank; the base is fixedly installed at the bottom of the culture tank, a sealing cover is hinged to the top of the culture tank, an observation opening is formed in the outer side of the culture tank, a glass panel is fixedly installed in the observation opening, a through groove is formed in the base, a gas inlet mechanism is arranged in the through groove, and the gas inlet mechanism is used for conveying gas with specific composition; the water tank is fixedly installed on the top of the base, and heating pieces are fixedly installed on the inner walls of the two sides of the water tank. In the using process, gas with specific composition can be evenly mixed with a culture solution conveniently, the culture solution can be evenly heated conveniently, and the device is simple in structure and convenient to use.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of stem cell culture, and particularly relates to a visual system tank for automatic culture of stem cells. BACKGROUND

[0002] There are many types of stem cells, and the stem cells have been widely applied in many fields such as disease treatment, medical beauty, anti-aging and drug screening. Stem cell culture has become a bottleneck restricting the development of the entire stem cell industry chain, and a stem cell culture device has become a development hotspot in the field of stem cell culture. A cell culture tank is a container for culturing cells. In the process of cell culture, the cell culture solution needs to be configured and then poured into the tank for long-time culture. In some cases, a stem cell system tank can be used for stem cell culture, especially when it is necessary to control the culture parameters and carry out large-scale production. For example, some complex bioreactor systems, such as perfusion reactors, can more effectively culture bone marrow mesenchymal stem cells and promote the proliferation and differentiation of cells. These systems can combine the storage function and culture function of the stem cell system tank to meet specific research or production needs.

[0003] The patent file with publication number CN209322909U discloses an automatic stirring type cell culture tank, which comprises a culture tank, a stirring motor arranged at the top of the culture tank, a stirring rod connected to the output end of the stirring motor, stirring blades arranged at the top end of the culture tank and penetrating the stirring rod, a partition plate arranged in the culture tank below the stirring blades, a liquid guide pipe communicated with the left end of the partition plate, a drainage rod arranged below the liquid guide pipe, a support column arranged at the bottom of the culture tank, an electric heating net arranged on the support column, a feeding port arranged in the left upper end of the sidewall of the culture tank, a material conveying cylinder arranged above the left of the culture tank, and a material conveying hose adapted to be plugged into the feeding port arranged at the right bottom end of the material conveying cylinder. The automatic stirring type cell culture tank effectively solves the problems of uneven stirring of the cell culture solution in the tank and uneven temperature in the culture process, ensures that the cell culture solution is subjected to uniform temperature, guarantees the temperature in the tank, realizes uniform and stable culture of the cell culture solution, and has the characteristics of simple operation and convenient use.

[0004] The patent file with publication number CN215162780U discloses a breathable stem cell culture tank, which comprises a breathable cylinder body instead of a traditional non-breathable glass cylinder body, a gas supply is strengthened through a jacket, and a breathable membrane outer support layer, a breathable membrane and a breathable membrane inner support layer constitute the breathable cylinder body. The jacket cylinder body is provided with an outer edge at both ends, and the breathable cylinder body is provided with an outer edge at the top end. The jacket cylinder body and the breathable cylinder body are assembled through a clamping plate, and the closed cavity between the jacket cylinder body and the breathable cylinder body is a gas control chamber. The gas composition and pressure of the gas control chamber are controlled through a gas inlet and a gas outlet. The gas input or output can be effectively controlled according to the needs of stem cell growth, and the stem cell production needs are met.

[0005] However, the above patent document is inconvenient for uniform mixing of gas with specific composition and culture solution during use, and is inconvenient for uniform heating of the culture solution, and therefore the visual system tank for automatic culture of stem cells is proposed to solve the above problems. Content of the utility model

[0006] The utility model discloses a visual system tank for automatic culture of stem cells, which is used to solve the problem that the system tank in the prior art is inconvenient for uniform mixing of gas with specific composition and culture solution during use and is inconvenient for uniform heating of the culture solution.

[0007] The visual system tank for automatic culture of stem cells provided by the application adopts the following technical scheme:

[0008] The visual system tank for automatic culture of stem cells comprises:

[0009] A culture tank;

[0010] A base is fixedly installed at the bottom of the culture tank, the top of the culture tank is hingedly connected with a sealing cover, an observation port is formed in the outer side of the culture tank, a glass panel is fixedly installed in the observation port, a through slot is formed in the base, and an air inlet mechanism is arranged in the through slot and used for conveying gas with specific composition;

[0011] A water tank is fixedly installed at the top of the base, heating fins are fixedly installed on the inner walls of the two sides of the water tank, a driving motor is fixedly installed at the top of the water tank, and a liquid inlet is formed in the outer side of the culture tank.

[0012] Further, the bottom inner wall of the culture tank is fixedly installed with a heating cylinder, the heating cylinder is provided with a heating cavity, the water tank is fixedly installed with a water inlet pipe and a water return pipe, the water inlet pipe and the water return pipe are fixedly communicated with the cavity, the side inner wall of the water tank is fixedly installed with a water pump, the output port of the water pump is fixedly communicated with one end of the water inlet pipe, and when the water pump is turned on, the water pump can convey the water in the water tank into the heating cylinder through the water inlet pipe.

[0013] Further, the outer side of the connecting pipe is fixedly connected with a plurality of second stirring blades, the plurality of second stirring blades are all provided with air passages, the plurality of second stirring blades are all fixedly installed with a plurality of nozzles, the nozzles are communicated with the air passages, the side inner walls of the plurality of air passages are all provided with air holes, the plurality of air holes are all communicated with the connecting pipe, and when the gas is conveyed through the air inlet pipe, the gas can be uniformly discharged through the connecting pipe, the plurality of air holes, the plurality of air passages and the plurality of nozzles.

[0014] Further, a third through hole is formed in the inner wall of one side of the through groove, an air inlet pipe is fixedly installed in the third through hole, the outer side of the air inlet pipe is slidably connected with the inner wall of the connecting pipe, an annular groove is formed in the inner wall of the connecting pipe, a sealing ring is rotatably installed in the annular groove, and the inner ring of the sealing ring is fixedly connected with the outer side of the air inlet pipe.

[0015] Further, a second through hole is formed in the inner wall of the top of the through groove, a connecting pipe is rotatably installed in the second through hole, a driving sprocket and a driven sprocket are fixedly installed on the outer side of the transmission rod and the outer side of the connecting pipe respectively, and the same chain is meshed on the driving sprocket and the driven sprocket.

[0016] Further, a first through hole is formed in the inner wall of the top of the through groove, a transmission rod is rotatably installed in the first through hole, one end of the transmission rod is fixedly connected with the output shaft of the driving motor, and a plurality of first stirring blades are fixedly connected with the outer side of the transmission rod.

[0017] To sum up, the present application has at least one of the following beneficial technical effects:

[0018] 1. When the driving motor and the water pump are turned on, the driving motor drives the transmission rod to rotate, the transmission rod drives the plurality of first stirring blades to rotate, the plurality of first stirring blades stir the water in the water tank to make it uniformly heated, and at the same time, the water pump circulates and delivers the water in the water tank to the heating cylinder through the water inlet pipe and the water return pipe, and the heating cylinder can uniformly heat the culture solution.

[0019] 2. When the specific gas is delivered through the air inlet pipe, the specific gas is discharged through the connecting pipe, the plurality of air holes, the plurality of air cavities and the plurality of nozzles, and at the same time, the transmission rod drives the driving sprocket to rotate, the driving sprocket drives the driven sprocket to rotate through the chain, and the driven sprocket drives the connecting pipe to rotate, so that the specific gas is uniformly discharged, and the mixing effect of the specific gas and the culture solution is improved.

[0020] The utility model discloses can be in the use process, it is convenient for making specific composition's gas and culture solution even mixing, and it is convenient to heat the culture solution evenly, simple structure, convenient to use. BRIEF DESCRIPTION OF DRAWINGS

[0021] Figure 1 It is the main view's structure schematic drawing of the visual system tank of stem cell automatic culture that the utility model puts forward;

[0022] Figure 2 It is the structure schematic drawing of the culture tank inside of the visual system tank of stem cell automatic culture that the utility model puts forward;

[0023] Figure 3 The structure diagram of the water tank inside the visual system tank for automatic cultivation of stem cells according to the utility model;

[0024] Figure 4 The structure diagram of the connecting pipe and the transmission rod of the visual system tank for automatic cultivation of stem cells according to the utility model;

[0025] Figure 5 The structure diagram of the second stirring blade of the visual system tank for automatic cultivation of stem cells according to the utility model;

[0026] Figure 6 The structure diagram of the connecting pipe and the air inlet pipe of the visual system tank for automatic cultivation of stem cells according to the utility model.

[0027] The figure mark: 1, the culture tank; 2, the base; 3, the water tank; 4, the sealing cover; 5, the liquid inlet; 6, the drive motor; 7, the heating cylinder; 8, the transmission rod; 9, the first stirring blade; 10, the water pump; 11, the water inlet pipe; 12, the backwater pipe; 13, the heating sheet; 14, the driving sprocket; 15, the chain; 16, the driven sprocket; 17, the connecting pipe; 18, the air inlet pipe; 19, the second stirring blade; 20, the nozzle; 21, the ventilation cavity; 22, the air hole; 23, the sealing ring; 24, the through slot. DETAILED DESCRIPTION

[0028] The technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model, and obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments.

[0029] Embodiment one

[0030] Referring to Figures 1-6 , the visual system tank for automatic cultivation of stem cells comprises:

[0031] The culture tank 1;

[0032] The base 2 is fixedly installed at the bottom of the culture tank 1, the top of the culture tank 1 is hingedly connected with the sealing cover 4, the outer side of the culture tank 1 is provided with the observation opening, the glass panel is fixedly installed in the observation opening, the through slot 24 is formed in the base 2, and the air inlet mechanism is arranged in the through slot 24, and the air inlet mechanism is used for conveying the gas with specific composition;

[0033] The water tank 3 is fixedly installed at the top of the base 2, the heating sheet 13 is fixedly installed on the inner wall of the water tank 3, the drive motor 6 is fixedly installed at the top of the water tank 3, and the liquid inlet 5 is formed in the outer side of the culture tank 1.

[0034] Referring to Figures 2-4The inner wall of the bottom of the culture tank 1 is fixedly provided with a heating cylinder 7, a heating cavity is formed in the heating cylinder 7, the water tank 3 is fixedly provided with a water inlet pipe 11 and a water return pipe 12, the water inlet pipe 11 and the water return pipe 12 are in fixed communication with the cavity, the inner wall of one side of the water tank 3 is fixedly provided with a water pump 10, the outlet of the water pump 10 is in fixed communication with one end of the water inlet pipe 11, when the water pump 10 is turned on, the water pump 10 can convey the water in the water tank 3 into the heating cylinder 7 through the water inlet pipe 11, a second through hole is formed in the top inner wall of the through groove 24, a connecting pipe 17 is rotatably installed in the second through hole, a driving sprocket 14 and a driven sprocket 16 are fixedly installed on the outer side of the transmission rod 8 and the outer side of the connecting pipe 17 respectively, the same chain 15 is engaged on the driving sprocket 14 and the driven sprocket 16, when the transmission rod 8 rotates, the driving sprocket 14 can drive the driven sprocket 16 to rotate through the chain 15, the top of the water tank 3 and the top inner wall of the through groove 24 are both provided with a first through hole, the same transmission rod 8 is rotatably installed in the two first through holes, one end of the transmission rod 8 is fixedly connected with the output shaft of the driving motor 6, a plurality of first stirring blades 9 are fixedly connected on the outer side of the transmission rod 8, when the driving motor 6 is turned on, the transmission rod 8 drives the plurality of first stirring blades 9 to rotate.

[0035] With reference to Figure 5 and Figure 6 A plurality of second stirring blades 19 are fixedly connected on the outer side of the connecting pipe 17, a plurality of ventilation cavities 21 are formed in the plurality of second stirring blades 19, a plurality of nozzles 20 are fixedly installed on the plurality of second stirring blades 19, the nozzles 20 are in communication with the ventilation cavities 21, a ventilation hole 22 is formed in the inner wall of one side of the plurality of ventilation cavities 21, the plurality of ventilation holes 22 are in communication with the connecting pipe 17, when the gas is conveyed through the air inlet pipe 18, the gas can be uniformly discharged through the connecting pipe 17, the plurality of ventilation holes 22, the plurality of ventilation cavities 21 and the plurality of nozzles 20, a third through hole is formed in the inner wall of one side of the through groove 24, the air inlet pipe 18 is fixedly installed in the third through hole, the outer side of the air inlet pipe 18 is in sliding connection with the inner wall of the connecting pipe 17, an annular groove is formed in the inner wall of the connecting pipe 17, a sealing ring 23 is rotatably installed in the annular groove, the inner ring of the sealing ring 23 is fixedly connected with the outer side of the air inlet pipe 18, when the connecting pipe 17 rotates, the sealing ring 23 can play a role in stabilizing the rotation of the connecting pipe 17.

[0036] The implementation principle of the visual system tank for automatic stem cell culture of the embodiment of the application is as follows: in use, the culture solution and stem cells are delivered into the culture tank 1 through the liquid inlet 5, the stem cell culture condition can be observed through the observation port, and meanwhile, the driving motor 6, the water pump 10 and the two heating fins 13 are turned on, the driving motor 6 drives the transmission rod 8 to rotate, the transmission rod 8 drives the plurality of first stirring blades 9 to rotate, the plurality of first stirring blades 9 stir the water in the water tank 3, the two heating fins 13 can uniformly heat the water in the water tank 3, the water pump 10 circulates and delivers the water in the water tank 3 to the heating cylinder 7 through the water inlet pipe 11 and the water return pipe 12, the heating cylinder 7 can uniformly heat the culture solution, then the specific gas is delivered through the air inlet pipe 18, the specific gas is discharged through the connecting pipe 17, the plurality of air holes 22, the plurality of air cavities 21 and the plurality of nozzles 20, the transmission rod 8 drives the driving sprocket 14 to rotate, the driving sprocket 14 drives the driven sprocket 16 to rotate through the chain 15, the driven sprocket 16 drives the connecting pipe 17 to rotate, the connecting pipe 17 drives the plurality of second stirring blades 19 to rotate, and thus the specific gas can be uniformly discharged, the mixing effect of the specific gas and the culture solution is improved, the stem cells can be rapidly exchanged with the gas, and the metabolic needs of the stem cells are met.

[0037] Embodiment two

[0038] The difference between the embodiment and the embodiment one is that the temperature sensor is fixedly installed in the cavity in the heating cylinder 7, the temperature controller is fixedly installed on the outer side of the water tank 3, the temperature sensor, the temperature controller and the two heating fins 13 are sequentially connected, the temperature sensor can monitor the internal temperature of the heating cylinder 7, when the water temperature in the heating cylinder 7 exceeds or is lower than the preset threshold value, the temperature sensor sends the instruction to the temperature controller, and the temperature controller can adjust the heating temperature of the two heating fins 13.

[0039] The above is only the preferred specific implementation method of the application, but the protection scope of the application is not limited to this, any skilled person in the art can make equivalent replacement or change according to the technical scheme and the application concept of the application within the technical range disclosed by the application, which should be covered in the protection scope of the application.

Claims

1. A visual system jar for automated stem cell culturing, characterized by: Include: Culture tank (1); Base (2) is fixedly installed at the bottom of the culture tank (1), the top of the culture tank (1) is hinged with a sealing cover (4), an observation port is formed in the outer side of the culture tank (1), a glass panel is fixedly installed in the observation port, a through slot (24) is formed in the base (2), and a gas inlet mechanism is arranged in the through slot (24), the gas inlet mechanism is used for conveying gas with a specific composition; Water tank (3) is fixedly installed on the top of the base (2), heating fins (13) are fixedly installed on the inner walls of the two sides of the water tank (3), a drive motor (6) is fixedly installed on the top of the water tank (3), and a liquid inlet (5) is formed in the outer side of the culture tank (1).

2. The automated stem cell culture visual system jar of claim 1, wherein: The top of the water tank (3) and the inner wall of the top of the through slot (24) are both provided with a first through hole, and the same transmission rod (8) is rotatably installed in the two first through holes, one end of the transmission rod (8) is fixedly connected with the output shaft of the drive motor (6), and a plurality of first stirring blades (9) are fixedly connected on the outer side of the transmission rod (8).

3. The automated stem cell culture visual system jar of claim 2, wherein: The bottom inner wall of the culture tank (1) is fixedly installed with a heating cylinder (7), the heating cylinder (7) is provided with a heating cavity, the water tank (3) is fixedly installed with a water inlet pipe (11) and a water return pipe (12), the water inlet pipe (11) and the water return pipe (12) are fixedly communicated with the cavity, and the water pump (10) is fixedly installed on the inner wall of one side of the water tank (3). The output port of the water pump (10) is fixedly communicated with one end of the water inlet pipe (11).

4. The automated stem cell culture visual system jar of claim 3, wherein: The top inner wall of the through slot (24) is provided with a second through hole, a connecting pipe (17) is rotatably installed in the second through hole, a driving sprocket (14) and a driven sprocket (16) are fixedly installed on the outer side of the transmission rod (8) and the outer side of the connecting pipe (17) respectively, and the same chain (15) is engaged on the driving sprocket (14) and the driven sprocket (16).

5. The automated stem cell culture visual system jar of claim 4, wherein: The outer side of the connecting pipe (17) is fixedly connected with a plurality of second stirring blades (19), a plurality of ventilation cavities (21) are formed in the plurality of second stirring blades (19), a plurality of nozzles (20) are fixedly installed on the plurality of second stirring blades (19), the nozzles (20) are communicated with the ventilation cavities (21), ventilation holes (22) are formed in the inner walls of one side of the plurality of ventilation cavities (21), and the plurality of ventilation holes (22) are communicated with the connecting pipe (17).

6. The automated stem cell culturing visual system jar of claim 5, wherein: The inner wall of one side of the through slot (24) is provided with a third through hole, the third through hole is fixedly installed with an air inlet pipe (18), the outer side of the air inlet pipe (18) is slidably connected with the inner wall of the connecting pipe (17), the inner wall of the connecting pipe (17) is provided with an annular groove, the annular groove is rotatably installed with a sealing ring (23), and the inner ring of the sealing ring (23) is fixedly connected with the outer side of the air inlet pipe (18).

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