PCR (Polymerase Chain Reaction) detection device

Abstract

The utility model discloses a PCR (Polymerase Chain Reaction) detection device and belongs to the technical field of PCR detection. The PCR detection device comprises a PCR carrying table, an unloading module and a pipetting mechanism; the PCR carrying table is provided with a recycling box, a PCR reaction reagent card and a standby suction head; the unloading module comprises a rotating plate, one end of the rotating plate is rotationally connected to the PCR carrying table, and the other end of the rotating plate can be switched between a first position and a second position, so that the rotating plate is located above the recycling box or located on one side of the recycling box; the pipetting mechanism comprises a motion module and a first Z-direction moving assembly connected to the output end of the motion module, the first Z-direction moving assembly can be connected with a standby suction head in an inserted mode so as to install the suction head, the suction head on the first Z-direction moving assembly can abut against the rotating plate at the first position, and the first Z-direction moving assembly drives the suction head to move upwards so as to unload the suction head; the suction head on the motion module can be in butt joint with the PCR reaction reagent card to transfer liquid, the replacement structure of the suction head is simple, and operation is convenient.

Description

TECHNICAL FIELD

[0001] The utility model relates to PCR detection technical field, especially PCR detection device. BACKGROUND

[0002] PCR detection is a kind of molecular biology technology for amplifying specific DNA fragments.PCR detection steps include: one, sample is put into protease and is mixed to form lysate, two, magnetic beads are put into lysate, three, magnetic beads are put into washing liquid and are washed (can be washed multiple times), four, magnetic beads are put into eluent and form to be detected liquid, five, to be detected liquid is transferred to PCR detector and is detected.The above process needs to transfer sample, to be detected liquid, washing liquid and eluent etc.Liquid.When using the same suction head to transfer liquid, the pollution between different liquids can be caused, and the detection result accuracy is influenced, so different suction heads need to be replaced to transfer liquid.In prior art, the replacement structure of suction head is complex, and inconvenient to operate. UTILITY MODEL CONTENT

[0003] The utility model aims at providing a kind of PCR detection device, and the replacement structure of suction head is simple, and convenient to operate.

[0004] To achieve this purpose, the utility model adopts the following technical solutions:

[0005] A kind of PCR detection device, comprising:

[0006] PCR platform is equipped with recovery tank, PCR reagent card and spare suction head;

[0007] Unloading module, including rotating plate, the rotating plate one end is rotatably connected to the PCR platform, and the other end can be switched between first position and second position;When first position, the rotating plate is located above the recovery tank;Second position, the rotating plate is located on one side of the recovery tank;

[0008] Pipetting mechanism, including movement module and the first Z direction movement module connected to the output end of movement module, the first Z direction movement module can be inserted with the spare suction head to install the suction head, the suction head on the first Z direction movement module can be abutted with the rotating plate of first position, the first Z direction movement module drives the suction head to move upwards to unload the suction head;The suction head on the movement module can be connected with the PCR reagent card to transfer liquid.

[0009] In some possible embodiments, the unloading module further comprises an unloading fixing base and an elastic member, the unloading fixing base is arranged on the PCR loading platform, the rotating plate is rotationally connected to the unloading fixing base, and the elastic member is connected between the rotating plate and the unloading fixing base, and the elastic member has a tendency to make the rotating plate be in the first position.

[0010] In some possible embodiments, the unloading fixing base is provided with a limiting slot, one end of the rotating plate is arranged in the limiting slot, and two side walls of the limiting slot are arranged at an angle, and two sides of the rotating plate can abut against the two side walls of the limiting slot to be in the first position and the second position.

[0011] In some possible embodiments, the suction head is provided with a first flange, and the first flange can abut against the bottom of the rotating plate in the first position.

[0012] In some possible embodiments, the rotating plate is provided with a limiting opening, the connection between the suction head and the first Z-direction moving module is limited in the limiting opening, and the first flange can abut against the edge of the limiting opening.

[0013] In some possible embodiments, the pipetting mechanism further comprises a power pump and a conduit, the conduit is arranged at the output end of the first Z-direction moving module, the suction head is inserted into one end of the conduit, and the other end of the conduit is connected to the power pump.

[0014] In some possible embodiments, the PCR loading platform is further provided with a spare magnetic sleeve, the output end of the motion module is further connected to a second Z-direction moving module, the magnetic sleeve can be inserted into the output end of the second Z-direction moving module, the magnetic sleeve on the second Z-direction moving module can abut against the rotating plate in the first position, the second Z-direction moving module drives the magnetic sleeve to move upward to unload the magnetic sleeve, and the magnetic sleeve on the motion module can be docked with the PCR reagent card to transfer magnetic beads.

[0015] In some possible embodiments, the output end of the second Z-direction moving module is connected to a Z-direction driving member and a rotating driving member, the output end of the Z-direction driving member is connected to a magnetic rod, the output end of the rotating driving member is connected to a sleeve, the sleeve can be inserted into the magnetic sleeve, and the magnetic rod is arranged in the sleeve and the magnetic sleeve.

[0016] In some possible embodiments, the magnetic sleeve is provided with a second flange, and the second flange can abut against the bottom of the rotating plate in the first position.

[0017] In some possible implementation manners, the PCR detection device is provided with a PCR detection port, the suction head on the motion module can be docked with the PCR detection port to transfer liquid, and the output end of the motion module is further connected with a heating assembly, and the heating assembly is used for heat sealing the PCR detection port.

[0018] The PCR detection device has the advantages that:

[0019] The PCR detection device has the advantages that: The suction head and the first Z-direction moving module are connected in a plug-in manner, and the suction head and the rotating plate are abutted, so that the suction head is moved upward to realize unloading and is convenient to disassemble. The unloading module is arranged on the PCR carrier, and only the movement position precision of the suction head is required to realize the matching precision of the rotating plate and the suction head, so that the reliability of the suction head unloading is ensured. When the rotating plate is located at the first position, the rotating plate is arranged above the recovery box, so that the suction head can be directly recovered after unloading. When the rotating plate is located at the second position, the rotating plate is located on one side of the recovery box, so that the recovery box can be taken out to be emptied, and subsequent use is facilitated. When the first Z-direction moving module drives the suction head to move downward, liquid can be sucked, and when the first Z-direction moving module drives the suction head to move upward, the suction head can be unloaded, and the driving function of the first Z-direction moving module is fully utilized. The above structure facilitates replacement of the suction head and emptying of the recovery box, and is simple in structure and convenient to operate. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 is a partial schematic view of the PCR detection device provided in the specific embodiment of the utility model;

[0021] Figure 2 is a schematic view of the pipetting mechanism provided in the specific embodiment of the utility model;

[0022] Figure 3 is a schematic view of the PCR detection mechanism provided in the specific embodiment of the utility model;

[0023] Figure 4 is a schematic view of the unloading module provided in the specific embodiment of the utility model.

[0024] In the drawings:

[0025] 100, frame body;

[0026] 200, pipetting mechanism; 210, movement module; 211, X direction movement module; 212, Y direction movement module; 230, second Z direction movement module; 240, first Z direction movement module; 250, magnetic sleeve; 251, second flange; 260, suction head; 261, first flange; 270, unloading module; 276, rotating plate; 2761, limiting port; 277, unloading fixed seat; 2771, limiting groove; 2772, upper seat; 2773, lower seat; 278, elastic piece; 281, guide pipe; 282, power pump; 291, sleeve pipe; 292, magnetic rod; 293, hollow brushless motor; 294, Z direction driving piece; 201, heating assembly; 202, third Z direction movement module;

[0027] 300, PCR detection mechanism; 310, PCR loading platform; 311, PCR reaction reagent card; 3111, first accommodating groove; 3112, second accommodating groove; 312, PCR detection reagent card; 313, PCR sample tube; 320, recovery box; 330, PCR detection port. DETAILED DESCRIPTION

[0028] In order to make the technical problems solved by the utility model, the technical scheme adopted and the technical effects reached more clear, the technical scheme of the embodiments of the utility model will be further described in detail below with reference to the drawings. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the protection scope of the utility model.

[0029] In the description of the utility model, unless there is explicit definition and limitation, the terms "connected", "connected", "fixed" should be understood broadly, for example, it can be fixed connection, or detachable connection, or integral; it can be mechanical connection, or electrical connection; it can be directly connected, or indirectly connected through intermediate medium, it can be the internal communication of two elements or the interaction relationship of two elements. For ordinary skilled in the art, the specific meaning of the above terms in the utility model can be understood according to the specific circumstances.

[0030] In the utility model, unless another definite provision and limitation, first feature is "on" or "under" second feature can include that first and second features are in direct contact, also can include that first and second features are not in direct contact but contact through other feature between them. Moreover, first feature "on", "above" and "upper surface of" second feature includes that first feature is directly above and obliquely above second feature, or only indicates that horizontal height of first feature is higher than second feature. First feature "under", "below" and "under surface of" second feature includes that first feature is directly below and obliquely below second feature, or only indicates that horizontal height of first feature is less than second feature.

[0031] As Figures 1-4 shown, the embodiment provides a PCR detection device, including PCR support 310, unloading module 270 and pipette mechanism 200.PCR support 310 is equipped with recovery tank 320, PCR reaction reagent card 311 and spare suction head 260.Unloading module 270 includes rotary plate 276, and rotary plate 276 one end is rotatably connected to PCR support 310, and the other end can be switched between first position and second position;When first position, rotary plate 276 is located above recovery tank 320;When second position, rotary plate 276 is located on one side of recovery tank 320.Pipette mechanism 200 includes movement module 210 and the first Z direction movement module 240 connected to the output end of movement module 210, and the first Z direction movement module 240 can be inserted with spare suction head 260 to install suction head 260, and the suction head 260 on the first Z direction movement module 240 can be abutted with rotary plate 276 when first position, and the first Z direction movement module 240 drives suction head 260 to move upward to unload suction head 260;The suction head 260 on movement module 210 can be docked with PCR reaction reagent card 311 to transfer liquid.Exemplarily, PCR detection device further includes frame body 100, and PCR support 310, unloading module 270 and pipette mechanism 200 are all installed on frame body 100.

[0032] Exemplarily, PCR reaction reagent card 311 is equipped with multiple first accommodating grooves 3111 and multiple second accommodating grooves 3112, and multiple first accommodating grooves 3111 and multiple second accommodating grooves 3112 are used for accommodating liquid such as washing liquid, eluent, reaction enzyme respectively, and pipette mechanism 200 moves suction head 260 in horizontal direction through movement module 210 to realize the docking with different accommodating grooves of PCR reaction reagent card 311, and the output of movement module 210 can be docked with spare suction head 260 or recovery tank 320.

[0033] The movement module 210 drives the first Z-direction movement module 240 and the suction head 260 to perform a liquid transfer. Then, the movement module 210 drives the first Z-direction movement module 240 and the suction head 260 to move to the position of the recycling box 320, so that the suction head 260 abuts against the rotating plate 276 at the first position. The first Z-direction movement module 240 drives the suction head 260 to move upward to unload the suction head 260. Since the rotating plate 276 at the first position is located above the recycling box 320, the suction head 260 can be directly recycled into the recycling box 320, which facilitates the disassembly and recycling of the suction head 260. The movement module 210 drives the first Z-direction movement module 240 to move to the standby suction head 260, and the suction head 260 is inserted into the first Z-direction movement module 240, which facilitates the insertion of the suction head 260, thereby achieving the replacement of the new suction head 260.

[0034] Optionally, the PCR sample tube 313 is further arranged on the PCR stage 310, and the suction head 260 can transfer the sample in the PCR sample tube 313 to the PCR reagent card 311.

[0035] The suction head 260 is inserted into the first Z-direction movement module 240, which facilitates the installation. The suction head 260 abuts against the rotating plate 276, and the suction head 260 moves upward to achieve unloading, which facilitates the disassembly. The unloading module 270 is arranged on the PCR stage 310, and only the movement position precision of the suction head 260 is required to achieve the cooperation precision between the rotating plate 276 and the suction head 260, which ensures the reliability of the unloading of the suction head 260. When the rotating plate 276 is at the first position, it is arranged above the recycling box 320, which facilitates the direct recycling of the suction head 260 after unloading. When the rotating plate 276 is at the second position, it is located on one side of the recycling box 320, and at this time, the recycling box 320 can be taken out to empty the recycling box 320, which facilitates the subsequent use. When the first Z-direction movement module 240 drives the suction head 260 downward, the suction head 260 can suck liquid. When the first Z-direction movement module 240 drives the suction head 260 upward, the suction head 260 can be unloaded, which fully utilizes the driving function of the first Z-direction movement module 240. Through the above structure, the replacement of the suction head 260 is facilitated, and the recycling box 320 is emptied, which is simple in structure and convenient to operate.

[0036] As Figure 4As shown, the unloading module 270 further comprises an unloading fixing seat 277 and an elastic member 278. The unloading fixing seat 277 is arranged on the PCR loading platform 310, and the rotating plate 276 is rotationally connected to the unloading fixing seat 277. The elastic member 278 is connected between the rotating plate 276 and the unloading fixing seat 277, and the elastic member 278 has a tendency to make the rotating plate 276 be located at the first position. That is, in the initial state of the elastic member 278, the rotating plate 276 is located above the recycling box 320. When the rotating plate 276 is pushed, the elastic member 278 is deformed, and the rotating plate 276 is located at the side of the recycling box 320, so as to facilitate taking out the recycling box 320. After the rotating plate 276 is released, the elastic member 278 resets, and the rotating plate 276 automatically resets to above the recycling box 320. Specifically, the elastic member 278 can be a spring.

[0037] The unloading fixing seat 277 is provided with a limiting groove 2771, one end of the rotating plate 276 is arranged in the limiting groove 2771, and the two side walls of the limiting groove 2771 are arranged at an included angle. The two sides of the rotating plate 276 can abut against the two side walls of the limiting groove 2771 respectively to be located at the first position and the second position. The rotating plate 276 is limited by the limiting groove 2771, the position accuracy of the rotating plate 276 is ensured, the recycling box 320 is facilitated to be taken out, and the cooperation accuracy of the rotating plate 276 and the suction head 260 is improved. Alternatively, the two side walls can be arranged at an included angle of 90° or 180°, and the like, which can be arranged according to the shape of the recycling box 320, and the like, without limitation. Alternatively, the unloading fixing seat 277 comprises an upper seat 2772 and a lower seat 2773, and the two are connected to limit the rotating plate 276 in the vertical direction.

[0038] The suction head 260 is provided with a first flange 261, and the first flange 261 can abut against the bottom of the rotating plate 276 in the first position. By arranging the first flange 261, the contact area of the suction head 260 and the rotating plate 276 is increased, and the unloading reliability is improved. Further, the first flange 261 is located at the top of the suction head 260.

[0039] The rotating plate 276 is provided with a limiting opening 2761, and the connection between the suction head 260 and the first Z-direction moving module 240 is limited in the limiting opening 2761. The first flange 261 can abut against the edge of the limiting opening 2761, so as to prevent the suction head 260 from being shaken and separated from the rotating plate 276 during the upward movement of the first Z-direction moving module 240, and ensure the connection reliability and tightness of the two, thereby improving the unloading reliability.

[0040] The pipetting mechanism 200 further comprises a power pump 282 and a conduit 281, the conduit 281 is arranged at the output end of the first Z-direction moving module 240, the tip 260 is inserted into one end of the conduit 281, and the other end of the conduit 281 is connected with the power pump 282. When the power pump 282 is started, the tip 260 is enabled to suck or release liquid through the conduit 281, so as to realize liquid transfer. The power pump 282 can be a micropump. The power pump 282 can be installed at the output end of the first Z-direction moving module 240 together with the conduit 281, so as to minimize the length of the conduit 281 and simplify the overall structure. Alternatively, the power pump 282 can be arranged externally, and only the conduit 281 is installed at the output end of the first Z-direction moving module 240, so as to reduce the load of the moving mechanism 210 and the first Z-direction moving module 240. The connection between the tip 260 and the first Z-direction moving module 240 is limited in the limiting opening 2761, specifically, the conduit 281 is limited in the limiting opening 2761.

[0041] During PCR detection, multiple magnetic beads need to be transferred. Therefore, the PCR stage 310 is further provided with a spare magnetic sleeve 250, and the output end of the moving mechanism 210 is further connected with a second Z-direction moving module 230. The magnetic sleeve 250 can be inserted into the output end of the second Z-direction moving module 230, the magnetic sleeve 250 on the second Z-direction moving module 230 can abut against the rotating plate 276 at the first position, and the second Z-direction moving module 230 drives the magnetic sleeve 250 to move upward to unload the magnetic sleeve 250; the magnetic sleeve 250 on the moving mechanism 210 can be docked with the PCR reagent card 311 to transfer the magnetic beads. The magnetic sleeve 250 can be used to transfer the magnetic beads, and the magnetic sleeve 250 can be replaced to avoid cross contamination. The spare magnetic sleeve 250 is inserted into the second Z-direction moving module 230, which is convenient for installation. The magnetic sleeve 250 abuts against the rotating plate 276, and the magnetic sleeve 250 moves upward to realize unloading, which is convenient for disassembly. The unloading module 270 is arranged on the PCR stage 310, and only the movement position precision of the magnetic sleeve 250 is required to realize the cooperation precision between the rotating plate 276 and the magnetic sleeve 250, so as to ensure the reliability of the unloading of the tip 260. When the rotating plate 276 is located at the first position, it is arranged above the recovery box 320, which is convenient for directly recovering the magnetic sleeve 250 after unloading. When the second Z-direction moving module 230 drives the magnetic sleeve 250 downward, the magnetic beads can be sucked, and when the second Z-direction moving module 230 drives the magnetic sleeve 250 upward, the magnetic sleeve 250 can be unloaded, so as to fully utilize the driving function of the second Z-direction moving module 230. The unloading module 270 can realize the unloading of the magnetic sleeve 250 and the tip 260, and the structure is simplified.

[0042] The output end of the second Z-direction movement module 230 is connected with a Z-direction driving member 294 and a rotating driving member. The output end of the Z-direction driving member 294 is connected with a magnetic rod 292. The output end of the rotating driving member is connected with a sleeve 291. The sleeve 291 can be plugged with the magnetic sleeve 250. The magnetic rod 292 penetrates the sleeve 291 and the magnetic sleeve 250. The output end of the second Z-direction movement module 230 is connected with a Z-direction driving member 294 and a rotating driving member. The output end of the Z-direction driving member 294 is connected with a magnetic rod 292. The output end of the rotating driving member is connected with a sleeve 291. The sleeve 291 can be plugged with the magnetic sleeve 250. The magnetic rod 292 penetrates the sleeve 291 and the magnetic sleeve 250. Specifically, the rotating driving member is a hollow brushless motor 293. The magnetic rod 292 penetrates the hollow brushless motor 293, the sleeve 291 and the magnetic sleeve 250. The Z-direction driving member 294 drives the magnetic rod 292 to move up and down. When the magnetic beads are taken out, the magnetic rod 292 moves downward and contacts the magnetic beads to achieve taking out. The magnetic rod 292 moves upward and separates from the magnetic beads to achieve releasing the magnetic beads. When the hollow brushless motor 293 drives the sleeve 291 and the magnetic sleeve 250 to rotate, the liquid can be mixed uniformly.

[0043] The magnetic sleeve 250 is provided with a second flange 251. The second flange 251 can abut against the bottom of the rotating plate 276 in the first position. By providing the second flange 251, the contact area of the magnetic sleeve 250 and the rotating plate 276 is increased, and the unloading reliability of the magnetic sleeve 250 is improved. The second flange 251 is provided on the top of the magnetic sleeve 250, which facilitates contact with the unloading member and is convenient and reliable to operate. When the second flange 251 abuts against the rotating plate 276, the connection between the magnetic sleeve 250 and the second Z-direction movement module 230, i.e., the sleeve 291, is limited in the limiting opening 2761. This prevents the magnetic sleeve 250 from shaking and separating from the rotating plate 276 during the upward movement of the second Z-direction movement module 230, thereby ensuring the connection reliability and tightness of the two, and improving the unloading reliability.

[0044] The PCR detection port 330 is arranged on the PCR carrier 310, and the suction head 260 on the motion module 210 can be docked with the PCR detection port 330 to transfer liquid, so as to realize PCR detection. Specifically, the PCR detection mechanism 300 in the prior art is arranged on the PCR carrier 310, and the PCR detection port 330 is a detection port of the PCR detection mechanism 300. The output end of the motion module 210 is further connected with a heating assembly 201, and the heating assembly 201 is used for heat sealing of the PCR detection port 330, so as to realize sealing of the PCR detection port 330, prevent pollution during detection, and improve detection accuracy. Optionally, the PCR detection port 330 is provided with a PCR detection reagent card 312, the suction head 260 transfers liquid into the PCR detection reagent card 312, and the heating assembly 201 can be a heat radiation component or the like, so as to realize melting and plugging of the PCR detection reagent card 312 after being heat radiated. Optionally, the output end of the motion module 210 is connected with a third Z-direction moving module 202, and the heating assembly 201 is connected to the output end of the third Z-direction moving module 202, so that the third Z-direction moving module 202 drives the heating assembly 201 to move upward or downward according to heating requirements.

[0045] The motion module 210 comprises an X-direction moving module 211 and a Y-direction moving module 212, the Y-direction moving module 212 is connected to the output end of the X-direction moving module 211, the output end of the Y-direction moving module 212 is connected with a support plate 213, and the first Z-direction moving module 240 and the second Z-direction moving module 230 are both connected to the support plate 213. The X-direction moving module 211, the Y-direction moving module 212, the first Z-direction moving module 240 and the second Z-direction moving module 230 can all adopt a linear module or a motor plus a screw nut pair structure in the prior art, and details are not repeated here.

[0046] Obviously, the above embodiments of the utility model are only examples for clearly explaining the utility model, and are not a limitation on the embodiments of the utility model. For ordinary skilled persons in the art, other different forms of changes or variations can be made on the basis of the above description. Here, all the embodiments are not enumerated. Any modification, equivalent replacement and improvement within the spirit and principle of the utility model should be included in the protection scope of the utility model claim.

Claims

1. A PCR detection device, characterized by, The PCR platform (310) is provided with a recovery box (320), a PCR reagent card (311) and a spare pipette tip (260). The unloading module (270) includes a rotating plate (276) which is rotatably connected to the PCR platform (310) at one end and can be switched between a first position and a second position; in the first position, the rotating plate (276) is located above the recovery box (320); in the second position, the rotating plate (276) is located on one side of the recovery box (320). The pipetting mechanism (200) includes a movement module (210) and a first Z-direction movement module (240) connected to the output end of the movement module (210); the first Z-direction movement module (240) can be inserted with the spare pipette tip (260) to install the pipette tip (260); the pipette tip (260) on the first Z-direction movement module (240) can abut against the rotating plate (276) in the first position; the first Z-direction movement module (240) drives the pipette tip (260) to move upward to unload the pipette tip (260); the pipette tip (260) on the movement module (210) can be docked with the PCR reagent card (311) to transfer liquid. The unloading module (270) further includes an unloading fixed seat (277) and an elastic member (278); the unloading fixed seat (277) is arranged on the PCR platform (310); the rotating plate (276) is rotatably connected to the unloading fixed seat (277); the elastic member (278) is connected between the rotating plate (276) and the unloading fixed seat (277); the elastic member (278) has a tendency to make the rotating plate (276) be in the first position.

2. The PCR detection device of claim 1, wherein, The unloading fixed seat (277) is provided with a limiting groove (2771); one end of the rotating plate (276) is arranged in the limiting groove (2771); the two side walls of the limiting groove (2771) are arranged at an angle; the two sides of the rotating plate (276) can abut against the two side walls of the limiting groove (2771) respectively to be in the first position and the second position.

3. The PCR detection device of claim 2, wherein, The pipette tip (260) is provided with a first flange (261); the first flange (261) can abut against the bottom of the rotating plate (276) in the first position.

4. The PCR detection device of claim 1, wherein, The rotating plate (276) is provided with a limiting port (2761); the connection between the pipette tip (260) and the first Z-direction movement module (240) is limited in the limiting port (2761); the first flange (261) can abut against the edge of the limiting port (2761).

5. The PCR detection device of claim 4, wherein, The pipetting mechanism (200) further includes a power pump (282) and a conduit (281); the conduit (281) is arranged at the output end of the first Z-direction movement module (240); the pipette tip (260) and one end of the conduit (281) are inserted; the other end of the conduit (281) is connected with the power pump (282).

6. The PCR detection device of claim 1, wherein, ​ 7. The PCR detection device of claim 1, wherein, The PCR carrier (310) is also provided with a spare magnetic sleeve (250), and the output end of the motion module (210) is also connected with a second Z-direction moving module (230), the magnetic sleeve (250) can be inserted into the output end of the second Z-direction moving module (230), the magnetic sleeve (250) on the second Z-direction moving module (230) can abut against the rotating plate (276) in the first position, and the second Z-direction moving module (230) drives the magnetic sleeve (250) to move upward to unload the magnetic sleeve (250); the magnetic sleeve (250) on the motion module (210) can be docked with the PCR reagent card (311) to transfer magnetic beads.

8. The PCR detection device of claim 7, wherein, The output end of the second Z-direction moving module (230) is connected with a Z-direction driving member (294) and a rotating driving member, the output end of the Z-direction driving member (294) is connected with a magnetic rod (292), and the output end of the rotating driving member is connected with a sleeve (291); the sleeve (291) can be inserted into the magnetic sleeve (250), and the magnetic rod (292) penetrates through the sleeve (291) and the magnetic sleeve (250).

9. The PCR detection device of claim 7, wherein, The magnetic sleeve (250) is provided with a second flange (251), and the second flange (251) can abut against the bottom of the rotating plate (276) in the first position.

10. The PCR detection device according to any one of claims 1-9, characterized in that, The PCR carrier (310) is provided with a PCR detection port (330), the suction head (260) on the motion module (210) can be docked with the PCR detection port (330) to transfer liquid, and the output end of the motion module (210) is also connected with a heating assembly (201), and the heating assembly (201) is used for heat sealing the PCR detection port (330).

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