PCR (Polymerase Chain Reaction) detection equipment

Abstract

The utility model discloses PCR (Polymerase Chain Reaction) detection equipment and belongs to the technical field of PCR detection. The PCR detection equipment comprises a PCR detection mechanism and a pipetting mechanism, the PCR detection mechanism comprises a PCR carrying table, the PCR carrying table is provided with a PCR detection port and a PCR reaction reagent card, the PCR detection port is provided with the PCR detection reagent card, the PCR detection reagent card is provided with an interface, the interface is provided with a flow guide part, a flow guide port of the flow guide part is communicated with the interface, and the cross-sectional area of the flow guide port of the flow guide part is larger than that of the interface; the pipetting mechanism comprises a movement module and a suction head and a heating assembly which are arranged on the movement module, the suction head can be in butt joint with the flow guide part and the PCR reaction reagent card to achieve liquid transfer, the heating assembly can heat the flow guide part to block the flow guide opening, the structure is simplified, operation steps are simplified, and use is convenient.

Description

TECHNICAL FIELD

[0001] The utility model relates to PCR detection technical field especially, relate to a PCR detection equipment. BACKGROUND

[0002] PCR detection is a kind of molecular biology technology for amplifying specific DNA fragments.PCR detection needs to transfer the liquid to be measured to the PCR detection port of PCR detector for detection.During detection, PCR detection port may be affected by contamination and affect the detection result, therefore, in the related art, film sealing machine is usually used to seal the PCR detection reagent card, and then the card is placed in the PCR detection port for detection, which has complex structure and is inconvenient to operate. UTILITY MODEL CONTENT

[0003] The utility model aims at providing a PCR detection equipment, simplify structure and simplify operation steps, convenient to use.

[0004] To achieve this purpose, the utility model adopts the following technical scheme:

[0005] A PCR detection equipment, comprising:

[0006] PCR detection mechanism, including PCR support, the PCR support is equipped with PCR detection port and PCR reaction reagent card, the PCR detection port is equipped with PCR detection reagent card, the PCR detection reagent card is equipped with interface, the interface is equipped with flow guide piece, the flow guide port of flow guide piece is communicated with the interface, the cross-sectional area of the flow guide port of flow guide piece is greater than the cross-sectional area of interface;

[0007] Pipetting mechanism, including movement module and the probe head and heating assembly on the movement module, the probe head can be respectively with flow guide piece and PCR reaction reagent card butt joint to realize liquid transfer, the heating assembly can heat flow guide piece to block flow guide port.

[0008] In some possible embodiments, the heating assembly is an electromagnetic heating coil.

[0009] In some possible embodiments, the PCR support is equipped with spare metal beads, the pipetting mechanism further includes magnetic sleeve on the movement module, the magnetic sleeve can transfer the metal beads to the flow guide port of flow guide piece, and the heating assembly can melt the metal beads to block the flow guide port.

[0010] In some possible implementation manners, the PCR detection reagent card is provided with a boss, the boss is provided with the interface, the flow guide piece comprises a through hole communicated with the flow guide port, the through hole is sleeved on the boss, a metal ring is arranged between the boss and the through hole, and the heating assembly can melt the metal ring to block the flow guide port.

[0011] In some possible implementation manners, the PCR carrier is provided with a first storage groove, a side wall of the first storage groove is provided with an elastic piece, the PCR reaction reagent card can be stored in the first storage groove, and the elastic piece elastically abuts against a side wall of the PCR reaction reagent card.

[0012] In some possible implementation manners, the PCR carrier is provided with a second storage groove, the second storage groove is provided with a consumable box, the consumable box stores a spare suction head, the PCR detection equipment further comprises a recycling box and an unloading module, the unloading module is used for unloading the suction head to the recycling box, and the movement module can be connected with the suction head of the consumable box.

[0013] In some possible implementation manners, the PCR carrier is further provided with a cover plate assembly, and the cover plate assembly can be arranged on the PCR detection reagent card.

[0014] In some possible implementation manners, the cover plate assembly comprises a cover plate body, a cover plate driving assembly and a cover plate support, the cover plate support is arranged on the PCR carrier, the cover plate body is rotationally connected to the cover plate support, the cover plate driving assembly can drive the cover plate body to rotate around an axis parallel to a surface of the PCR carrier, and the cover plate body can be arranged on the PCR detection reagent card.

[0015] In some possible implementation manners, the pipetting mechanism is arranged on the frame body, and the PCR carrier is slidingly connected to the frame body and can be arranged inside the frame body or outside the frame body.

[0016] In some possible implementation manners, the drawer driving assembly is further arranged on the frame body and is used for driving the PCR carrier to move on the frame body.

[0017] The utility model discloses the beneficial effects of:

[0018] The utility model provides a PCR detection equipment, and the pipette is taken out from PCR reaction reagent card and is put into PCR detection reagent card through flow guide spare, namely, the measured liquid is transferred to PCR detection port and carries out PCR detection. Through setting flow guide spare, because the cross section area of flow guide port of flow guide spare is greater than the cross section area of interface, flow guide port has the flow guide effect, and it is convenient to transfer the measured liquid to PCR detection reagent card. And heating assembly can heat flow guide spare and realize the plugging of flow guide port, and then the interface of PCR detection reagent card is plugged, realizes sealed detection, avoids the influence of the detection result by the pollution, and avoids destroying PCR detection reagent card and PCR detection port. Because the pipette and heating assembly are all arranged on the movement module, need not to set sealing machine additionally, simplifies the structure, and heating assembly directly heats and plugs the flow guide spare on the PCR detection reagent card of PCR detection port, need not to transfer PCR detection reagent card to sealing machine and seal, then transfers PCR detection reagent card from sealing machine to PCR detection port, simplifies the operation step, and is convenient to use. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is the partial schematic view of PCR detection equipment provided by the specific embodiment of the utility model,

[0020] Figure 2 It is the schematic diagram of pipetting mechanism provided by the specific embodiment of the utility model,

[0021] Figure 3 It is the schematic diagram of PCR detection mechanism provided by the specific embodiment of the utility model,

[0022] Figure 4 It is the schematic diagram of PCR detection reagent card, flow guide spare and metal bead provided by the specific embodiment of the utility model,

[0023] Figure 5 It is the schematic diagram of PCR detection reagent card, flow guide spare and metal ring provided by the specific embodiment of the utility model.

[0024] In the drawing,

[0025] 100, frame body,

[0026] 200, pipetting mechanism, 210, movement module, 211, X direction movement module, 212, Y direction movement module, 230, second Z direction movement module, 240, first Z direction movement module, 250, magnetic sleeve, 251, second flange, 260, pipette, 261, first flange, 270, unloading module, 281, catheter, 282, power pump, 291, sleeve, 292, magnetic bar, 293, hollow brushless motor, 294, Z direction driving part, 201, heating assembly, 202, third Z direction movement module,

[0027] 300, PCR detection mechanism; 310, PCR stage; 311, PCR reagent card; 312, PCR detection reagent card; 3121, interface; 3122, boss; 313, PCR sample tube; 314, first storage slot; 315, elastic member; 316, second storage slot; 317, third storage slot; 318, consumable box; 320, recycling box; 330, PCR detection port; 350, flow guide member; 351, flow guide port; 352, metal beads; 353, metal ring; 360, cover plate assembly; 361, cover plate body; 362, cover plate driving assembly; 363, cover plate support; 370, drawer driving assembly. DETAILED DESCRIPTION

[0028] To make the technical problems solved by the present application, the technical solutions adopted and the technical effects achieved more clear, the technical solutions of the embodiments of the present application will be further described in detail below with reference to the drawings. Obviously, the described embodiments are only part of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0029] In the description of the present application, unless otherwise explicitly specified and limited, the terms "connected", "connected", "fixed" should be understood broadly, for example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the internal communication of two elements or the interaction relationship between two elements. For ordinary skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0030] In the present application, unless otherwise explicitly specified and limited, the first feature "on" or "below" the second feature can include that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, the first feature "on", "above" and "above" the second feature includes that the first feature is directly above and obliquely above the second feature, or only indicates that the horizontal height of the first feature is higher than that of the second feature. The first feature "below", "below" and "below" the second feature includes that the first feature is directly below and obliquely below the second feature, or only indicates that the horizontal height of the first feature is less than that of the second feature.

[0031] As Figures 1-5As shown, the embodiment provides a PCR detection device, which comprises a PCR detection mechanism 300 and a pipetting mechanism 200. The PCR detection mechanism 300 comprises a PCR carrier 310, wherein the PCR carrier 310 is provided with a PCR detection port 330 and a PCR reagent card 311, the PCR detection port 330 is provided with a PCR detection reagent card 312, the PCR detection reagent card 312 is provided with an interface 3121, the interface 3121 is provided with a flow guide piece 350, a flow guide port 351 of the flow guide piece 350 is in communication with the interface 3121, and a cross-sectional area of the flow guide port 351 of the flow guide piece 350 is greater than that of the interface 3121. The pipetting mechanism 200 comprises a movement module 210 and a suction head 260 and a heating assembly 201 arranged on the movement module 210, the suction head 260 can be respectively docked with the flow guide piece 350 and the PCR reagent card 311 to realize liquid transfer, and the heating assembly 201 can heat the flow guide piece 350 to block the flow guide port 351.

[0032] The PCR detection mechanism 300 comprises a PCR detector arranged on the PCR carrier 310, the PCR detector is a prior art, and the PCR detection port 330 is a detection port on the PCR detector. The suction head 260 takes out the to-be-tested liquid from the PCR reagent card 311 and puts it into the PCR detection reagent card 312 through the flow guide piece 350, that is, the to-be-tested liquid is transferred to the PCR detection port 330 for PCR detection. By arranging the flow guide piece 350, since the cross-sectional area of the flow guide port 351 of the flow guide piece 350 is greater than that of the interface 3121, the flow guide port 351 has a flow guide effect, which facilitates the transfer of the to-be-tested liquid to the PCR detection reagent card 312. Moreover, the heating assembly 201 can heat the flow guide piece 350 to block the flow guide port 351, thereby blocking the interface 3121 of the PCR detection reagent card 312, realizing sealed detection, avoiding pollution and affecting the detection result, and avoiding damage to the PCR detection reagent card 312 and the PCR detection port 330. Since the suction head 260 and the heating assembly 201 are both arranged on the movement module 210, there is no need to additionally arrange a film sealing machine, the structure is simplified, and the heating assembly 201 directly heats and blocks the flow guide piece 350 on the PCR detection reagent card 312 located at the PCR detection port 330, without the need to transfer the PCR detection reagent card 312 to the film sealing machine for sealing, and then transfer the PCR detection reagent card 312 from the film sealing machine to the PCR detection port 330, thereby simplifying the operation steps and facilitating use.

[0033] Optionally, the PCR detection reagent card 312 is provided with a boss 3122, the boss 3122 is provided with the interface 3121, the flow guide piece 350 comprises a through hole in communication with the flow guide port 351, and the through hole is sleeved on the boss 3122, thereby facilitating the communication between the flow guide piece 350 and the interface 3121 on the PCR detection reagent card 312. Specifically, the flow guide port 351 is a tapered port.

[0034] Optionally, the heating assembly 201 is an electromagnetic heating coil, and the flow guide 350 is blocked by heat radiation.

[0035] As shown in the first embodiment, Figure 4 the PCR platform 310 is provided with spare metal beads 352, and the pipetting mechanism 200 further comprises a magnetic sleeve 250 arranged on the movement module 210, which can transfer the metal beads 352 to the flow guide port 351 of the flow guide 350. The heating assembly 201 can melt the metal beads 352 to block the flow guide port 351, further improving the blocking effect. The metal beads 352 can be steel beads. Optionally, the PCR reagent card 311 is provided with a plurality of liquid accommodating grooves, such as lysis solution, washing solution or elution solution, etc. The magnetic beads are transferred to the above-mentioned liquid accommodating grooves by the magnetic sleeve 250 to realize related operations such as sample purification.

[0036] As shown in the second embodiment, Figure 5 the boss 3122 and the through hole are provided with a metal ring 353, and the heating assembly 201 can melt the metal ring 353 to block the flow guide port 351, further improving the blocking effect. The metal ring 353 can be a steel ring.

[0037] As shown in the second embodiment, Figure 3 the PCR platform 310 is further provided with a cover plate assembly 360, which can be arranged on the PCR detection reagent card 312. After the flow guide 350 is blocked, the cover plate assembly 360 is arranged on the PCR detection reagent card 312, further preventing the PCR detection reagent card 312 from being contaminated.

[0038] The cover plate assembly 360 comprises a cover plate body 361, a cover plate driving assembly 362 and a cover plate support 363. The cover plate support 363 is arranged on the PCR platform 310, and the cover plate body 361 is rotationally connected to the cover plate support 363. The cover plate driving assembly 362 can drive the cover plate body 361 to rotate around an axis parallel to the platform surface of the PCR platform 310, and the cover plate body 361 can be arranged on the PCR detection reagent card 312. Optionally, the cover plate body 361 can be rotated by 180°. Before being arranged, one side of the cover plate body 361 is supported on the PCR platform 310 and arranged on one side of the PCR detection reagent card 312. Then, the cover plate driving assembly 362 drives the cover plate body 361 to rotate by 180°, so that the other side of the cover plate body 361 is arranged on the PCR detection reagent card 312. Optionally, the cover plate body 361 is provided with a avoiding groove, which can avoid the flow guide 350 to prevent structural interference when the flow guide 350 and the PCR detection reagent card 312 protrude from the PCR platform 310. The cover plate driving assembly 362 can be a rotary air cylinder or a motor, or can also be a motor connected with a belt transmission assembly, which is connected with the cover plate body 361 to realize the rotation of the cover plate body 361.

[0039] As shown in Figure 1 and Figure 3 The first storage groove 314 is arranged on the PCR carrier 310, the side wall of the first storage groove 314 is provided with an elastic member 315, the PCR reagent card 311 can be stored in the first storage groove 314, the elastic member 315 elastically abuts against the side wall of the PCR reagent card 311, so as to ensure that the PCR reagent card 311 is stably and reliably installed on the PCR carrier 310, and the installation precision is ensured, and the liquid taking by the suction head 260 is facilitated. Alternatively, the PCR carrier 310 includes a flat plate structure and a groove structure, the groove structure is installed at the bottom of the flat plate structure, and the two are connected to form the first storage groove 314.

[0040] The PCR carrier 310 is provided with a second storage groove 316, the second storage groove 316 is provided with a consumable box 318, the consumable box 318 stores spare suction heads 260, the PCR detection equipment further includes a recycling box 320 and an unloading module 270, the unloading module 270 is used for unloading the suction head 260 to the recycling box 320, and the movement module 210 can be connected with the suction head 260 of the consumable box 318. By setting the unloading module 270, the suction head 260 is unloaded to the recycling box 320, and then the spare suction head 260 in the consumable box 318 is installed on the movement module 210, so as to realize the replacement of the suction head 260, different suction heads 260 are used to transfer different liquids, cross contamination of the liquids is prevented, and the detection reliability is improved. Specifically, the suction head 260 is inserted at the end of the movement module 210, so as to facilitate installation and unloading.

[0041] Alternatively, the PCR carrier 310 is provided with a third storage groove 317, and the recycling box 320 is arranged in the third storage groove 317, so as to facilitate taking out the recycling box 320 for emptying.

[0042] Alternatively, the PCR carrier 310 is provided with a PCR sample tube 313, and the suction head 260 can take out the sample in the PCR sample tube 313 and transfer the sample to the PCR reagent card 311, and the operation is specifically performed according to actual detection.

[0043] The motion module 210 comprises an X-direction moving module 211 and a Y-direction moving module 212. The Y-direction moving module 212 is connected to the output end of the X-direction moving module 211. The output end of the Y-direction moving module 212 is connected with a support plate. The first Z-direction moving module 240, the second Z-direction moving module 230 and the third Z-direction moving module 202 are all connected to the support plate. The output end of the first Z-direction moving module 240 is connected with the suction head 260. The output end of the second Z-direction moving module 230 is connected with the magnetic sleeve 250. The output end of the third Z-direction moving module 202 is connected with the heating assembly 201. In this way, the suction head 260, the magnetic sleeve 250 and the heating assembly 201 can work independently and avoid interference. The X-direction moving module 211, the Y-direction moving module 212, the first Z-direction moving module 240, the second Z-direction moving module 230 and the third Z-direction moving module 202 can all adopt the linear module or the motor plus the screw nut pair structure in the prior art, and thus will not be described herein.

[0044] The upper portion of the recycling box 320 is provided with an unloading sheet. The suction head 260 and the magnetic sleeve 250 can abut against the bottom of the unloading sheet, respectively. The first Z-direction moving module 240 drives the suction head 260 to move upward. The second Z-direction moving module 230 drives the magnetic sleeve 250 to move upward, so as to realize unloading. Alternatively, the top of the suction head 260 is provided with a first flange 261. The top of the magnetic sleeve 250 is provided with a second flange 251. The first flange 261 and the second flange 251 are arranged to abut against the unloading sheet, respectively, so as to increase the contact area and improve the unloading reliability.

[0045] The pipetting mechanism 200 further comprises a power pump 282 and a conduit 281. The conduit 281 is arranged at the output end of the first Z-direction moving module 240. The suction head 260 and one end of the conduit 281 are connected in a plug-in manner. The other end of the conduit 281 is connected with the power pump 282. When the power pump 282 is started, the suction head 260 is caused to suck or release liquid through the conduit 281, so as to realize liquid transfer. The power pump 282 can be a micropump. The power pump 282 can be installed at the output end of the first Z-direction moving module 240 together with the conduit 281, so as to minimize the length of the conduit 281 and simplify the overall structure. Alternatively, the power pump 282 can be arranged externally, and only the conduit 281 is installed at the output end of the first Z-direction moving module 240, so as to reduce the load of the motion module 210 and the first Z-direction moving module 240. The connection position between the suction head 260 and the first Z-direction moving module 240 is limited in a limiting opening. Specifically, the conduit 281 is limited in the limiting opening.

[0046] The output end of the second Z-direction moving module 230 is connected with a Z-direction driving member 294 and a rotating driving member. The output end of the Z-direction driving member 294 is connected with a magnetic rod 292. The output end of the rotating driving member is connected with a sleeve 291. The sleeve 291 can be plugged with the magnetic sleeve 250. The magnetic rod 292 penetrates the sleeve 291 and the magnetic sleeve 250. The output end of the second Z-direction moving module 230 is connected with the Z-direction driving member 294 and the rotating driving member. The output end of the Z-direction driving member 294 is connected with the magnetic rod 292. The output end of the rotating driving member is connected with the sleeve 291. The sleeve 291 can be plugged with the magnetic sleeve 250. The magnetic rod 292 penetrates the sleeve 291 and the magnetic sleeve 250. Specifically, the rotating driving member is a hollow brushless motor 293. The magnetic rod 292 penetrates the hollow brushless motor 293, the sleeve 291 and the magnetic sleeve 250. The Z-direction driving member 294 drives the magnetic rod 292 to move up and down. When the magnetic beads or the metal beads 352 are taken out, the magnetic rod 292 moves downward and contacts the magnetic beads or the metal beads 352 to achieve taking out. The magnetic rod 292 moves upward and separates from the magnetic beads to achieve releasing the magnetic beads or the metal beads 352. When the hollow brushless motor 293 drives the sleeve 291 and the magnetic sleeve 250 to rotate, liquid mixing can be achieved.

[0047] The PCR detection device further comprises a rack 100. The pipetting mechanism 200 is arranged on the rack 100. The PCR platform 310 is slidingly connected to the rack 100 and can be placed inside or outside the rack 100. When the PCR platform 310 is located outside the rack 100, the PCR reagent card 311 and the PCR detection reagent card 312 can be placed on the PCR platform 310. After the reaction, the PCR reagent card 311 and the PCR detection reagent card 312 can be taken out. When the PCR platform 310 is located inside the rack 100, liquid transfer and detection of the to-be-detected liquid can be performed. Before and after the reaction, the PCR platform 310 can be pulled out, which facilitates the placement and removal of the PCR reagent card 311 and the PCR detection reagent card 312.

[0048] The PCR detection device further comprises a drawer driving assembly 370. The drawer driving assembly 370 is used to drive the PCR platform 310 to move on the rack 100, so as to realize automatic pulling out of the PCR platform 310. Illustratively, the drawer driving assembly 370 comprises a motor and a screw nut pair. The motor can be a servo motor, which improves the moving precision of the PCR platform 310 and further facilitates cooperation with the pipetting mechanism 200 to realize liquid transfer. Alternatively, the PCR platform 310 moves on the rack 100 along a guide structure, such as a guide rail and a sliding block connected to the guide rail. The guide rail is fixed on the rack 100. The sliding block is connected to the PCR platform 310.

[0049] Obviously, the above embodiments of the present application are merely examples for clearly illustrating the present application, and are not intended to limit the implementation modes of the present application. For those skilled in the art, other different forms of changes or variations can be made on the basis of the above description. Here, it is not necessary and also impossible to exhaust all the implementation modes. Any modification, equivalent replacement and improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application claims.

Claims

1. A PCR detection device, characterized in that, include: A PCR testing apparatus (300) includes a PCR stage (310), wherein the PCR stage (310) is provided with a PCR testing port (330) and a PCR reaction reagent card (311), wherein the PCR testing port (330) is provided with a PCR testing reagent card (312), wherein the PCR testing reagent card (312) is provided with an interface (3121), wherein the interface (3121) is provided with a flow guide (350), wherein the flow guide port (351) of the flow guide (350) is connected to the interface (3121), and the cross-sectional area of ​​the flow guide port (351) of the flow guide (350) is larger than the cross-sectional area of ​​the interface (3121); The pipetting mechanism (200) includes a motion module (210) and a pipette tip (260) and a heating component (201) disposed on the motion module (210). The pipette tip (260) can be docked with the flow guide (350) and the PCR reaction reagent card (311) respectively to realize liquid transfer. The heating component (201) can heat the flow guide (350) to block the flow port (351).

2. The PCR detection device according to claim 1, characterized in that, The heating component (201) is an electromagnetic heating coil.

3. The PCR detection device according to claim 2, characterized in that, The PCR stage (310) is equipped with spare metal beads (352), and the pipetting mechanism (200) further includes a magnetic sleeve (250) on the motion module (210). The magnetic sleeve (250) can transfer the metal beads (352) to the flow port (351) of the flow guide (350), and the heating component (201) can melt the metal beads (352) to seal the flow port (351).

4. The PCR detection device according to claim 2, characterized in that, The PCR test reagent card (312) is provided with a boss (3122), and the boss (3122) is provided with the interface (3121). The flow guide (350) includes a through hole communicating with the flow guide port (351). The through hole is sleeved on the boss (3122). A metal ring (353) is provided between the boss (3122) and the through hole. The heating component (201) can melt the metal ring (353) to block the flow guide port (351).

5. The PCR detection device according to claim 1, characterized in that, The PCR stage (310) is provided with a first storage slot (314), and the side wall of the first storage slot (314) is provided with an elastic element (315). The PCR reaction reagent card (311) can be stored in the first storage slot (314), and the elastic element (315) elastically abuts against the side wall of the PCR reaction reagent card (311).

6. The PCR detection device according to claim 1, characterized in that, The PCR stage (310) is provided with a second storage slot (316), and a consumable box (318) is provided on the second storage slot (316). The consumable box (318) stores spare pipette tips (260). The PCR detection device also includes a recycling bin (320) and an unloading module (270). The unloading module (270) is used to unload the pipette tips (260) to the recycling bin (320). The motion module (210) can be connected to the pipette tips (260) in the consumable box (318).

7. The PCR detection device according to claim 1, characterized in that, The PCR stage (310) is also provided with a cover plate assembly (360), which can cover the PCR test reagent card (312).

8. The PCR detection device according to claim 7, characterized in that, The cover plate assembly (360) includes a cover plate body (361), a cover plate driving assembly (362), and a cover plate support (363). The cover plate support (363) is disposed on the PCR stage (310). The cover plate body (361) is rotatably connected to the cover plate support (363). The cover plate driving assembly (362) can drive the cover plate body (361) to rotate about an axis parallel to the surface of the PCR stage (310). The cover plate body (361) can cover the PCR test reagent card (312).

9. The PCR detection device according to any one of claims 1-8, characterized in that, It also includes a frame (100), the pipetting mechanism (200) is disposed on the frame (100), and the PCR stage (310) is slidably connected to the frame (100) and can be placed inside the frame (100) or outside the frame (100).

10. The PCR detection device according to claim 9, characterized in that, It also includes a drawer drive assembly for driving the PCR stage (310) to move on the frame (100).

Images