Three-dimensional cell culture bioreactor
By introducing a speed-regulating motor and stirring structure into a three-dimensional cell culture bioreactor, combined with limiting and heat preservation measures, the problem of time-consuming and labor-intensive dynamic growth environment simulation in existing technologies has been solved, achieving stable slow stirring and environmental control, and improving culture efficiency.
Patent Information
- Application Number
- CN202423090037.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-13
- Publication Date
- 2025-11-28
- Estimated Expiration
- 2034-12-13
AI Technical Summary
Existing three-dimensional cell culture bioreactors are time-consuming and labor-intensive in simulating dynamic growth environments, which affects the culture progress.
A bioreactor was designed, comprising a culture substrate, a cap, a speed-regulating motor, a connecting rod, a stirring rod, and stirring blades. The speed-regulating motor is controlled by adjusting a knob, which drives the stirring rod and stirring blades to perform slow stirring. Combined with structures such as limiting blocks, heat insulation cotton, and waterproof membrane, stability and environmental control are ensured.
This technology enables stable, slow stirring of three-dimensional cells, simulating a suitable growth environment, improving culture efficiency, and enhancing the stability and environmental control of the equipment.
Smart Images
Figure CN223607289U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to cell culture device technical field, specifically is a kind of three-dimensional cell culture bioreactor. BACKGROUND
[0002] Three-dimensional cell culture technology refers to the common culture of animal cells and scaffold materials with three-dimensional structure, so that cells can grow, proliferate and migrate in three-dimensional space, form three-dimensional cell or cell carrier complex, and better simulate the growth environment of cells in vivo.
[0003] Three-dimensional cell culture bioreactor is a device for simulating the growth environment of cells in vivo in vitro. By constructing three-dimensional space structure, cells can grow, reproduce and interact in it, so as to better simulate the physiological function and interaction of cells. This technology has wide application in drug research and development, tissue engineering and other fields.
[0004] After long-term use, it is found that the existing three-dimensional cell culture bioreactor provides a static environment suitable for the growth and reproduction of three-dimensional cells. To form a dynamic growth environment that needs to be stirred, researchers need to perform corresponding operations to achieve it. This not only wastes time and effort, but also affects the progress of three-dimensional cell culture.
[0005] Therefore, the utility model provides a three-dimensional cell culture bioreactor. UTILITY MODEL CONTENT
[0006] In order to make up for the shortcomings of the prior art and solve at least one technical problem raised in the background art.
[0007] The utility model solves the technical problems by adopting the following technical scheme: the utility model discloses a three-dimensional cell culture bioreactor, which comprises a bottom plate; a culture tank is formed in the middle of the culture bottom plate; the culture tanks are evenly distributed in the middle of the culture bottom plate; a cover is slidably connected to the top of the culture bottom plate; a cover is slidably connected to the middle of the cover; the cover is provided with a plurality of cover covers; the cover cover corresponds to the position of the adjusting knob; a speed regulating motor is fixedly connected to the inner side wall of the cover cover; a connecting rod is connected to the output end of the speed regulating motor; a stirring rod is fixedly connected to the end of the connecting rod; a stirring fin is fixedly connected to the side wall of the stirring rod; an adjusting knob is fixedly connected to the adjusting end of the speed regulating motor. Through the above structure, the culture bottom plate and the culture tank can be placed in the three-dimensional cell culture bioreactor. The speed of the speed regulating motor can be adjusted by the adjusting knob. The stirring rod and the stirring fin can be driven by the connecting rod to slowly stir the three-dimensional cell culture bioreactor, simulate the culture environment suitable for the growth of some three-dimensional cell culture, and strengthen the slow stirring adjustment effect of the three-dimensional cell culture bioreactor.
[0008] Preferably, the culture bottom plate top is fixed with a stable seat; the stable seat is provided with a plurality of; each of the stable seat inside wall is provided with a clamping groove; the cover cap outside wall is fixed with a limiting block; the limiting block is uniformly distributed on the cover cap outside wall; the clamping groove and the cover cap and the limiting block are slidingly matched; through the above structure, the stable seat and the clamping groove can be used for limiting and fixing the cover cap by the limiting block, so that the cover cap is more stable when the rotating speed adjusting motor is used, and the slow rotation stability of the three-dimensional cell culture bioreactor is further enhanced.
[0009] Preferably, the inside wall of the cover is fixed with heat preservation cotton; the bottom of the heat preservation cotton is fixed with a waterproof film; through the above structure, the heat preservation cotton and the waterproof film can be used for heat preservation and waterproof between the culture bottom plate and the cover, so as to avoid the influence of temperature change and water loss on the three-dimensional cell culture, and further enhance the heat preservation and waterproof effect of the three-dimensional cell culture bioreactor.
[0010] Preferably, the outside wall of the adjusting knob is fixed with a blocking ring; the blocking ring is located above the limiting block; through the above structure, the blocking ring can be used for blocking the cover cap when the cover cap is moved, so as to avoid the damage of the cover cap sliding to the three-dimensional cell organism, and further enhance the moving blocking protection effect of the three-dimensional cell culture bioreactor.
[0011] Preferably, the outside wall of the cover is fixed with a hanging ring; the hanging ring is uniformly distributed on the outside wall of the cover; through the above structure, the hanging ring can make the movement of the cover more stable, and the contact between the culture bottom plate and the culture groove inside wall is less, and the transfer stability of the three-dimensional cell culture bioreactor is further enhanced.
[0012] Preferably, the outside wall of the culture bottom plate is provided with a timer; through the above structure, the timer can be used for recording the use time of the rotating speed adjusting motor, and the stirring time of the three-dimensional cell culture is recorded, and the stirring time of the three-dimensional cell culture bioreactor is further enhanced.
[0013] Preferably, the outside wall of the cover is provided with a level instrument; through the above structure, the level instrument can be used for detecting the horizontal state of the cover, avoiding the uneven stirring caused by the inclination, and further enhancing the horizontal detection effect of the three-dimensional cell culture bioreactor.
[0014] The beneficial effects of the utility model are as follows:
[0015] 1. The utility model relates to a three-dimensional cell culture bioreactor, the setting of culture bottom plate and culture tank can place the three-dimensional cell biology that needs to be cultured, the setting of adjusting knob can adjust the rotating speed of rotating speed adjusting motor, three-dimensional cell biology can be stirred slowly by connecting rod driving stirring rod and stirring wing piece through rotating speed adjusting motor, simulate some three-dimensional biological cell suitable growth culture environment, strengthen the slow stirring adjustment effect of three-dimensional cell culture bioreactor.
[0016] 2. The utility model relates to a three-dimensional cell culture bioreactor, the setting of stabilizing seat and card slot can utilize the limiting block and fix with limit to cover, make the cover more stable when rotating speed adjusting motor is used, further strengthen the slow rotation stability effect of three-dimensional cell culture bioreactor. ACCURACY OF DRAWINGS
[0017] The utility model will be further described below in combination with the drawings.
[0018] Figure 1 It is the perspective drawing of the utility model;
[0019] Figure 2 It is the structure diagram of the unfolding cover of the utility model;
[0020] Figure 3 It is the structure diagram of the card slot of the utility model;
[0021] Figure 4 It is the structure diagram of the rail of the utility model.
[0022] In the drawing: 1, culture bottom plate;11, culture tank;12, cover;13, rotating speed adjusting motor;14, connecting rod;15, stirring rod;16, stirring wing piece;17, adjusting knob;18, cover;2, stabilizing seat;21, card slot;22, limiting block;3, heat preservation cotton;31, waterproof membrane;4, blocking ring;5, hanging ring;6, timer;7, level. DETAILED DESCRIPTION
[0023] In order to make the technical means, creative characteristics, achieve the purpose and function of the utility model easy to understand, the utility model is further described below in combination with specific implementation ways.
[0024] For example Figures 1 to 4As shown in the utility model embodiment, the three-dimensional cell culture bioreactor comprises a culture base plate 1, a culture groove 11 is formed in the middle of the culture base plate 1, the culture grooves 11 are uniformly distributed in the middle of the culture base plate 1, a cover 12 is slidably connected to the top of the culture base plate 1, a cover cap 18 is slidably connected to the middle of the cover 12, the cover cap 18 is provided in plurality, the cover cap 18 corresponds to the position of an adjusting knob 17, a rotating speed adjusting motor 13 is fixedly connected to the inner side wall of the cover cap 18, a connecting rod 14 is connected to the output end of the rotating speed adjusting motor 13, a stirring rod 15 is fixedly connected to the end of the connecting rod 14, stirring wings 16 are fixedly connected to the side wall of the stirring rod 15, the adjusting knob 17 is fixedly connected to the adjusting end of the rotating speed adjusting motor 13, during operation, the three-dimensional cell organisms to be cultured can be placed in the culture groove 11, the connecting rod 14 is rotated by the rotating speed adjusting motor 13, and then the stirring rod 15 and the stirring wings 16 are driven to stir the three-dimensional cell organisms placed in the culture groove 11, the rotating speed of the rotating speed adjusting motor 13 is adjusted by the adjusting knob 17, the stirring rod 15 and the stirring wings 16 slowly stir the three-dimensional organisms, the growth environment of some special three-dimensional cell organisms is simulated, the influence of external factors on the rotation of the rotating speed adjusting motor 13 is reduced by the cover cap 18, the three-dimensional cell organisms to be cultured can be placed by the culture base plate 1 and the culture groove 11, the rotating speed of the rotating speed adjusting motor 13 is adjusted by the adjusting knob 17, the stirring rod 15 and the stirring wings 16 are driven by the connecting rod 14 to slowly stir the three-dimensional cell organisms, the culture environment suitable for the growth of some three-dimensional cell organisms is simulated, and the slow stirring adjustment effect of the three-dimensional cell culture bioreactor is improved.
[0025] As Figures 1 to 4 shown, the culture base plate 1 is fixedly connected with a stabilizing seat 2 at the top, the stabilizing seat 2 is provided in plurality, the inner side wall of each stabilizing seat 2 is formed with a clamping groove 21, a limiting block 22 is fixedly connected to the outer side wall of the cover cap 18, the limiting blocks 22 are uniformly distributed on the outer side wall of the cover cap 18, the clamping groove 21 is slidably connected with the cover cap 18 and the limiting block 22, during operation, the cover cap 18 is fixedly limited by the limiting block 22 through the sliding connection of the clamping groove 21, the cover cap 18, and the limiting block 22, so that the cover cap 18 is prevented from being displaced and dislocated when the stirring rod 15 and the stirring wings 16 are rotated by the rotating speed adjusting motor 13, and unnecessary damage to the three-dimensional cell organisms is avoided, the cover cap 18 is fixedly limited by the limiting block 22 through the stabilizing seat 2 and the clamping groove 21, so that the cover cap 18 is more stable when the rotating speed adjusting motor 13 is used, and the slow rotation stability effect of the three-dimensional cell culture bioreactor is further improved.
[0026] As Figures 1 to 4As shown, the inner side wall of the cover 12 is fixed with the heat preservation cotton 3; the bottom of the heat preservation cotton 3 is fixed with the waterproof film 31; during operation, the temperature between the culture bottom plate 1 and the cover 12 can be maintained through the heat preservation cotton 3, and the waterproof film 31 can reduce the liquid splashed by the stirring rod 15 and the stirring wing 16 from entering the inside of the heat preservation cotton 3 for dyeing; through the arrangement of the heat preservation cotton 3 and the waterproof film 31, the culture bottom plate 1 and the cover 12 can be kept warm and waterproof, avoiding the influence of temperature change and water loss on three-dimensional cell biological culture, and further enhancing the heat preservation and waterproof effect of the three-dimensional cell culture bioreactor.
[0027] As shown in the figure, Figures 1 to 2 The outer side wall of the adjusting knob 17 is fixed with the blocking ring 4; the blocking ring 4 is located above the limiting block 22; during operation, the blocking ring 4 can be used to limit the cover 18 when all the covers 18 are removed by the cover 12, avoiding the damage to the three-dimensional cell organisms caused by the sliding of the cover 18; through the arrangement of the blocking ring 4, the cover 18 can be blocked when the cover 12 is moved, avoiding the damage to the three-dimensional cell organisms caused by the sliding of the cover 18, and further enhancing the moving blocking protection effect of the three-dimensional cell culture bioreactor.
[0028] As shown in the figure, Figures 1 to 3 The outer side wall of the cover 12 is fixed with the hanging ring 5; the hanging ring 5 is evenly distributed on the outer side wall of the cover 12; during operation, the hanging ring 5 evenly distributed on the outer side wall of the cover 12 can be used to stably transfer the cover 12 as a whole by using the lifting rope, avoiding the knocking of the inner side wall of the culture tank 11 when the cover 12 is moved; through the arrangement of the hanging ring 5, the movement of the cover 12 can be more stable, and the contact between the culture bottom plate 1 and the inner side wall of the culture tank 11 can be less, further enhancing the transfer stability effect of the three-dimensional cell culture bioreactor.
[0029] As shown in the figure, Figures 1 to 2 The outer side wall of the culture bottom plate 1 is installed with the timer 6; during operation, the timer 6 can record the use time of the rotating speed adjusting motor 13, facilitating the time recording of the three-dimensional cell biological culture; through the arrangement of the timer 6, the use time of the rotating speed adjusting motor 13 can be recorded, facilitating the recording of the stirring time of the three-dimensional cell biological culture, and further enhancing the stirring time statistics of the three-dimensional cell culture bioreactor.
[0030] As shown in the figure, Figures 1 to 2 The outer side wall of the cover 12 is installed with the level 7; during operation, the level 7 can detect the horizontal state of the cover 12, avoiding the unstable stirring of the stirring rod 15 and the stirring wing 16 caused by the inclination of the cover 12; through the arrangement of the level 7, the horizontal state of the cover 12 can be detected, avoiding the uneven stirring caused by the inclination, and further enhancing the horizontal detection effect of the three-dimensional cell culture bioreactor.
[0031] When working, the three-dimensional cell organisms to be cultured can be placed in the culture tank 11, the connecting rod 14 can be rotated by the rotating speed adjusting motor 13, and then the stirring rod 15 and the stirring blade 16 are driven to stir the three-dimensional cell organisms placed in the culture tank 11, the rotating speed of the rotating speed adjusting motor 13 can be adjusted by the adjusting knob 17, so that the stirring rod 15 and the stirring blade 16 slowly stir the three-dimensional organisms, simulate the growth environment of some special three-dimensional cell organisms, the covering cover 18 can reduce the influence of external factors on the rotation of the rotating speed adjusting motor 13, the sliding cooperation of the clamping groove 21, the covering cover 18 and the limiting block 22 can use the limiting block 22 to limit and fix the covering cover 18, avoid the displacement of the covering cover 18 when the stirring rod 15 and the stirring blade 16 are rotated by the rotating speed adjusting motor 13, and cause unnecessary harm to the three-dimensional biological cells, the temperature between the culture bottom plate 1 and the cover 12 can be maintained by the heat preservation cotton 3, the waterproof film 31 can reduce the liquid splashed by the stirring rod 15 and the stirring blade 16 from entering the heat preservation cotton 3, the blocking ring 4 can be limited when the cover 12 is used to remove all the covering covers 18, avoid the damage to the three-dimensional cell organisms caused by the sliding of the covering cover 18, the hanging ring 5 uniformly distributed on the outer side wall of the cover 12 can use the lifting rope to stably transfer the cover 12 as a whole, avoid the knocking of the culture tank 11 inner side wall when moving the cover 12, the timer 6 can record the use time of the rotating speed adjusting motor 13, facilitate the time record of the three-dimensional cell organism culture, and the level meter 7 can detect the horizontal state of the cover 12, avoid the inclination of the cover 12 to cause the unstable stirring of the stirring rod 15 and the stirring blade 16.
[0032] The basic principle, main features and advantages of the present application are shown and described above. It should be understood by those skilled in the art that the present application is not limited by the above embodiments, and the above embodiments and descriptions in the specification are only to illustrate the principles of the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the claimed present application. The scope of protection of the present application is defined by the appended claims and their equivalents.
Claims
1. A three-dimensional cell culture bioreactor comprising a culture floor (1); characterized in that: The culture bottom plate (1) is provided with culture grooves (11) in the middle part; the culture grooves (11) are uniformly distributed in the middle part of the culture bottom plate (1); the culture bottom plate (1) is slidably connected with a cover (12) on the top; the cover (12) is slidably connected with a cover cover (18) in the middle part; the cover cover (18) is provided with a plurality of; the cover cover (18) corresponds to the position of the adjusting knob (17); the cover cover (18) is fixedly connected with a rotating speed adjusting motor (13) on the inner side wall bottom; the output end of the rotating speed adjusting motor (13) is connected with a connecting rod (14); the end of the connecting rod (14) is fixedly connected with a stirring rod (15); the side wall of the stirring rod (15) is fixedly connected with a stirring fin (16); the adjusting knob (17) is fixedly connected with the adjusting knob (17).
2. A three-dimensional cell culture bioreactor according to claim 1, c h a r a c t e r i z e d in that The culture bottom plate (1) is fixedly connected with a stabilizing seat (2) on the top; the stabilizing seat (2) is provided with a plurality of; the inner side wall of each stabilizing seat (2) is provided with a clamping groove (21); the outer side wall of the cover cover (18) is fixedly connected with a limiting block (22); the limiting block (22) is uniformly distributed on the outer side wall of the cover cover (18); the clamping groove (21) is slidably connected with the cover cover (18) and the limiting block (22).
3. The three-dimensional cell culture bioreactor of claim 1, wherein: The inner side wall of the cover (12) is fixedly connected with thermal insulation cotton (3); the bottom of the thermal insulation cotton (3) is fixedly connected with a waterproof film (31).
4. The three-dimensional cell culture bioreactor of claim 1, wherein: The outer side wall of the adjusting knob (17) is fixedly connected with a blocking ring (4); the blocking ring (4) is located above the limiting block (22).
5. The three-dimensional cell culture bioreactor of claim 1, wherein: The outer side wall of the cover (12) is fixedly connected with a hanging ring (5); the hanging ring (5) is uniformly distributed on the outer side wall of the cover (12).
6. The three-dimensional cell culture bioreactor of claim 1, wherein: The culture bottom plate (1) is provided with a timer (6) on the outer side wall.
7. The three-dimensional cell culture bioreactor of claim 1, wherein: The outer side wall of the cover (12) is provided with a level (7).