Hypoxia condition incubator for umbilical cord mesenchymal stem cell amplification

Abstract

The utility model is applicable to the field of stem cell hypoxia culture, and provides a hypoxia condition incubator for umbilical cord mesenchymal stem cell amplification, which comprises a culture tank and a culture component arranged on the culture tank, the culture tank comprises a tank body and a sealing door, an opening frame is arranged on the side surface of the tank body, and the sealing door is arranged on the tank body. Two extrusion strips are fixedly and radially mounted on the inner side of the opening frame, the culture assembly comprises a motor, a culture column and a culture box, the surface of the culture column is extruded through the extrusion strips, and the space in the tank body is divided into an inner area and an outer area according to whether the space is in direct contact with the outside or not; the incubator needing to be inspected is rotated to the outer area, the sealing door is opened to inspect the incubator, the oxygen leakage condition of the inner area is reduced, and it is guaranteed that the incubator in the inner area is in a low-oxygen state all the time.

Description

TECHNICAL FIELD

[0001] The utility model belongs to stem cell hypoxic culture field especially relates to a kind of umbilical cord mesenchymal stem cell expansion's low oxygen condition incubator. BACKGROUND

[0002] Stem cell is a kind of special cell with self-replication ability and multi-directional differentiation potential, is widely used in regenerative medicine and research.For the stem cell extracted from human body, low oxygen environment is the basic condition commonly seen in human body.In vitro artificial manufacturing this low oxygen environment, the effect of culturing and expanding stem cell is usually more ideal.

[0003] In the expansion culture of umbilical cord mesenchymal stem cell, stem cell state is usually needed to be checked regularly, so the closed culture device is often needed to be opened, so that the oxygen environment in culture device changes, and then it can affect the propagation and expansion of stem cell. UTILITY MODEL CONTENT

[0004] The utility model embodiment purposes to provide a kind of umbilical cord mesenchymal stem cell expansion's low oxygen condition incubator, to solve the problem presented in background art.

[0005] The utility model embodiment is realized as follows, a kind of umbilical cord mesenchymal stem cell expansion's low oxygen condition incubator, including culture tank and the culture assembly being installed on culture tank, the culture tank includes jar body and sealing door, the opening frame is set in the side surface of jar body, the support leg is fixedly installed in the lower end of jar body, the sealing door is hingedly fixed in the side surface of opening frame, the sealing ring is fixedly installed in the side surface of sealing door, two extrusion strips are fixedly installed in the inside of opening frame, the culture assembly includes motor, culture column and incubator, the motor is fixedly installed in the middle part of upper end of jar body, the culture column is movably installed in jar body, the end of two extrusion strips is extruded contact with the surface of culture column, the motor spindle is movably penetrated jar body and is extended to jar body and is fixedly connected with culture column, the three culture grooves are fixedly set in the side surface of culture column, the culture groove includes a plurality of drawer grooves arranged longitudinally, the incubator is movably installed in drawer groove.

[0006] As a further scheme of the utility model, the middle part of upper end of culture column is fixedly set with fixed groove, the fixed disc is fixedly arranged on the motor spindle, and the fixed disc is fixedly installed in fixed groove.

[0007] As a further scheme of the utility model, the middle part of lower end of culture column is fixedly set with limit groove, the inside bottom of jar body is set as limit shaft corresponding to limit groove, and the limit shaft is clamped in limit groove.

[0008] As a further scheme of the utility model: the culture column corresponds to the extrusion strip and is fixedly installed with multiple sealing strips on the side surface, the extrusion strip and the sealing strip are both set as elastic material, the distance between the sealing strip and the adjacent sealing strip is same with the distance between the two top ends of the extrusion strips.

[0009] As a further scheme of the utility model: the incubator is set as a frame body, and the incubator is fixedly provided with air holes and buckle grooves on one side.

[0010] As a further scheme of the utility model: the tank body is fixedly connected with an exhaust port at the lower end, and is fixedly connected with an air inlet at the upper end, and the air inlet is connected with external air supply equipment.

[0011] The utility model discloses beneficial effect lies in:

[0012] The low-oxygen condition incubator for umbilical cord mesenchymal stem cell expansion provided by the utility model embodiment, through extruding the surface of culture column by extrusion strip, the space in tank body is divided into two areas according to whether directly contact with outside, when needing to check incubator, by rotating culture column, the incubator needing to check is rotated to outer area, and the sealing door is opened to check the incubator, which reduces the oxygen leakage in inner area, and guarantees that the incubator in inner area is always in low-oxygen state.

[0013] The low-oxygen condition incubator for umbilical cord mesenchymal stem cell expansion provided by the utility model embodiment, through the limiting shaft being clamped into the limiting slot, the culture column is limited, the coaxiality of culture column rotation is guaranteed, and the culture column is supported, the motor spindle load is reduced, and energy is saved.

[0014] The low-oxygen condition incubator for umbilical cord mesenchymal stem cell expansion provided by the utility model embodiment, through the sealing strip, the culture column can be positioned when rotating, and when the sealing strip extrudes the surface of extrusion strip, the sealing strip can further improve the sealing effect.

[0015] The low-oxygen condition incubator for umbilical cord mesenchymal stem cell expansion provided by the utility model embodiment, through the air inlet being arranged, oxygen-containing air is introduced into the tank body, the oxygen concentration in oxygen-containing air is adjusted, the oxygen concentration in the tank body is adjusted, the exhaust port is arranged, and the adjustment of the air inlet is assisted. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 The utility model provides a kind of low-oxygen condition incubator for umbilical cord mesenchymal stem cell expansion provided by the utility model embodiment, and its sectional view is shown in the figure;

[0017] Figure 2 The utility model provides a kind of low-oxygen condition incubator for umbilical cord mesenchymal stem cell expansion provided by the utility model embodiment, and its sectional view is shown in the figure;Figure 1 ;

[0018] Figure 3 The utility model provides a section of umbilical cord mesenchymal stem cell expansion's low oxygen condition incubator of providing for the embodiment of the utility model Figure 2 .

[0019] In the drawing: culture jar 1, jar body 11, open frame 111, support leg 112, exhaust port 113, air inlet 114, limiting shaft 115, sealing door 12, sealing ring 13, extrusion strip 14, culture assembly 2, motor 21, fixed disc 211, culture column 22, culture groove 221, drawer groove 222, fixed groove 223, limiting groove 224, incubator 23, air hole 231, buckle groove 232, sealing strip 24. DETAILED DESCRIPTION

[0020] In order to make the utility model purposes, technical scheme and advantages more clearly, following combining with the drawing and example, the utility model is further detailedly explained. It should be understood that the specific example described here is only used to explain the utility model, and is not used to limit the utility model.

[0021] The specific implementation of the utility model is described in detail below in combination with specific examples.

[0022] As Figures 1 to 3 As shown in the figure, a kind of umbilical cord mesenchymal stem cell expansion's low oxygen condition incubator provided for the embodiment of the utility model, including culture jar 1 and installation on culture jar 1 culture assembly 2, the culture jar 1 includes jar body 11 and sealing door 12, the jar body 11 side is equipped with open frame 111, the jar body 11 lower end is fixedly installed with support leg 112, the sealing door 12 is hingedly fixed in open frame 111 side, the sealing door 12 side is fixedly installed with sealing ring 13, two extrusion strips 14 are fixedly installed with radial in open frame 111, the culture assembly 2 includes motor 21, culture column 22 and incubator 23, the motor 21 is fixedly installed on jar body 11 upper end middle part, the culture column 22 is movably installed in jar body 11, two the end of extrusion strip 14 and culture column 22 surface extrusion contact, the motor 21 main shaft movably penetrates jar body 11 and extends to jar body 11 inside and is fixedly connected with culture column 22, three culture grooves 221 are fixedly provided on the side surface of culture column 22, the culture groove 221 includes multiple drawer grooves 222 arranged longitudinally, the incubator 23 is movably installed in drawer groove 222.

[0023] In one embodiment of the present application, the surface of the culture column 22 is extruded by the extrusion strip 14, and the space in the tank body 11 is divided into two regions according to whether it is directly contacted with the outside world. When the incubator needs to be checked, the incubator 23 to be checked is rotated to the outer region by rotating the culture column 22, and the sealing door 12 is opened to check the incubator 23, thereby reducing the leakage of oxygen in the inner region and ensuring that the incubator in the inner region is always in a low-oxygen state.

[0024] As shown in Figure 3 As another embodiment of the present application, a fixed groove 223 is formed in the middle of the upper end of the culture column 22, a fixed disc 211 is fixedly arranged on the main shaft of the motor 21, and the fixed disc 211 is fixedly installed in the fixed groove 223.

[0025] In one embodiment of the present application, the fixed disc 211 is installed in the fixed groove 223, which facilitates the transmission of the torque of the motor 21, reduces the transmission loss, and further facilitates the control of the rotation angle of the culture column 22 by controlling the rotation of the motor 21.

[0026] As shown in Figure 3 As another embodiment of the present application, a limiting groove 224 is formed in the middle of the lower end of the culture column 22, a limiting shaft 115 is arranged on the inner bottom of the tank body 11 corresponding to the limiting groove 224, and the limiting shaft 115 is clamped in the limiting groove 224.

[0027] In one embodiment of the present application, the limiting shaft 115 is clamped in the limiting groove 224, which limits the culture column 22, ensures the coaxiality of the rotation of the culture column 22, simultaneously supports the culture column 22, reduces the load of the main shaft of the motor 21, and saves energy.

[0028] As shown in Figure 1 and Figure 2 As another embodiment of the present application, a plurality of sealing strips 24 are fixedly installed on the side surface of the culture column 22 corresponding to the extrusion strip 14, the extrusion strip 14 and the sealing strip 24 are both made of elastic material, and the distance between the sealing strip 24 and the adjacent sealing strip 24 is the same as the distance between the top ends of the two extrusion strips 14.

[0029] In one embodiment of the present application, the sealing strip 24 can be positioned when the culture column 22 rotates, and when the sealing strip 24 extrudes the surface of the extrusion strip 14, it can also assist the extrusion strip 14 to further improve the sealing effect.

[0030] As shown in Figure 2 and Figure 3As another embodiment of the utility model, the incubator 23 is provided as a frame body, and the incubator 23 is fixed with a ventilation hole 231 and a buckle groove 232 on one side.

[0031] In an embodiment of the utility model, the buckle groove 232 can be used to pull out the incubator 23 from the drawer groove 222, and the embedded design ensures the flatness of the surface of the incubator 23, thereby avoiding affecting the sealing effect of the extrusion strip 14 extruding the surface of the culture column 22 when the culture column 22 rotates.

[0032] As shown in the figure, Figure 1 As another embodiment of the utility model, the tank body 11 is fixedly connected with an exhaust port 113 at the lower end, and the tank body 11 is fixedly connected with an air inlet 114 at the upper end, and the air inlet 114 is connected with an external air supply device.

[0033] In an embodiment of the utility model, the oxygen-containing air is introduced into the tank body 11 through the air inlet 114, the oxygen concentration in the oxygen-containing air is adjusted, thereby adjusting the oxygen concentration in the tank body 11, and the exhaust port 113 is used to exhaust the gas, which can also assist the adjustment of the air inlet 114.

[0034] For those skilled in the art, it is obvious that the present application is not limited to the details of the above-mentioned exemplary embodiments, and the present application can be realized in other specific forms without departing from the spirit or essential characteristics of the present application. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting, and the scope of the present application is defined by the appended claims rather than the above description, and therefore all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present application. Any reference signs in the claims should not be regarded as limiting the claims involved.

[0035] It should be noted that the embodiments in the utility model and the features in the embodiments can be combined with each other without conflict.

[0036] In the description of the utility model, need understanding is, the term "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "internal", "external" and so on indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawing, just for the convenience of describing the utility model and simplifying the description, and not indicate or imply that the device or element indicated must have a particular orientation, construct and operate in a particular orientation, therefore can not be understood as the restriction of the utility model. In addition, the term "first", "second" and so on are only for the purpose of description, and can not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features limited by "first", "second" and so on can explicitly or implicitly include one or more features. In the description of the utility model, unless otherwise specified, the meaning of "a plurality of" is two or more.

[0037] In the description of the utility model, it should be explained that, unless otherwise specified and limited, the terms "mounting", "connecting", "connection" should be understood broadly, for example, can be fixed connection, can also be detachable connection, or integrally connected, can be mechanical connection, can also be electrical connection, can be directly connected, can also be indirectly connected through intermediate medium, can be the communication inside two elements. For ordinary skilled in the art, the specific meaning of the above terms in the utility model can be understood through specific circumstances.

[0038] In addition, it should be understood that, although the present specification is described according to the embodiments, not every embodiment contains only one independent technical scheme, the description manner of the specification is only for clarity, the skilled in the art should regard the specification as a whole, and the technical scheme in each embodiment can also be appropriately combined to form other embodiments that the skilled in the art can understand.

Claims

1. An umbilical cord mesenchymal stem cell expansion hypoxic condition incubator, comprising a culture tank (1) and a culture assembly (2) installed on the culture tank (1), characterized in that, The culture tank (1) includes a tank body (11) and a sealing door (12), the tank body (11) is provided with an opening frame (111) on the side, the tank body (11) is fixedly installed with a supporting leg (112) at the lower end, the sealing door (12) is hingedly fixed on the side of the opening frame (111), the sealing door (12) is fixedly installed with a sealing ring (13) on the side, two extrusion strips (14) are fixedly and radially installed in the inside of the opening frame (111), the culture assembly (2) includes a motor (21), a culture column (22) and a culture box (23), the motor (21) is fixedly installed on the upper end of the tank body (11), the culture column (22) is movably installed in the tank body (11), the end portions of the two extrusion strips (14) are in extrusion contact with the surface of the culture column (22), the main shaft of the motor (21) movably penetrates the tank body (11) and extends into the tank body (11) and is fixedly connected with the culture column (22), the culture column (22) is fixedly provided with three culture grooves (221) on the side, the culture groove (221) includes a plurality of longitudinally arranged drawer grooves (222), and the culture box (23) is movably installed in the drawer groove (222).

2. The hypoxic condition incubator for expanding umbilical cord mesenchymal stem cells according to claim 1, wherein, The culture column (22) is fixedly provided with a fixed groove (223) in the middle of the upper end, and the motor (21) is fixedly provided with a fixed disc (211) on the main shaft, and the fixed disc (211) is fixedly installed in the fixed groove (223).

3. The hypoxic condition incubator for expanding umbilical cord mesenchymal stem cells according to claim 2, wherein, The culture column (22) is fixedly provided with a limiting groove (224) in the middle of the lower end, and the inside bottom of the tank body (11) is provided with a limiting shaft (115) corresponding to the limiting groove (224), and the limiting shaft (115) is clamped in the limiting groove (224).

4. The hypoxic condition incubator for expansion of umbilical cord mesenchymal stem cells according to claim 1, wherein, The culture column (22) is fixedly installed with a plurality of sealing strips (24) on the side corresponding to the extrusion strip (14), and the extrusion strip (14) and the sealing strip (24) are both made of elastic material, and the distance between the sealing strips (24) and the adjacent sealing strips (24) is the same as the distance between the top ends of the two extrusion strips (14).

5. The hypoxic condition incubator for expansion of umbilical cord mesenchymal stem cells according to claim 1, wherein, The culture box (23) is provided as a frame body, and the culture box (23) is fixedly provided with a ventilation hole (231) and a buckle groove (232) on one side.

6. The hypoxic condition incubator for expansion of umbilical cord mesenchymal stem cells according to claim 1, wherein, The tank body (11) is fixedly connected with an exhaust port (113) at the lower end, and the tank body (11) is fixedly connected with an air inlet (114) at the upper end, and the air inlet (114) is connected with an external air supply device.

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