Transfer film box
By designing a transfer box containing multiple drawers and an ice box, the problems of complexity and stability in multi-batch sample transfer operations were solved, achieving an efficient and stable protein transfer process.
Patent Information
- Application Number
- CN202520252362.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-17
- Publication Date
- 2025-12-02
- Estimated Expiration
- 2035-02-17
AI Technical Summary
In existing Western blotting experiments, the transfer of multiple batches of samples is complex and requires a high level of proficiency, and the high temperature environment affects the stability of protein transfer.
Design a transfer box containing multiple drawers and an ice box to provide a low-temperature environment. The temperature of the transfer solution is controlled by inlet and outlet valves to ensure that reagents and consumables are prepared at low temperatures and to support simultaneous operation of multiple batches.
It enables efficient and stable protein transfer processes in multiple batches, improving transfer stability and ease of operation, and reducing protein transfer loss.
Smart Images
Figure CN223624220U_ABST
Abstract
Description
Technical Field
[0001] This utility model belongs to the field of experimental instrument technology, specifically relating to a transfer box. Background Technology
[0002] Western blotting (WB), also known as protein blotting, is a method for detecting a specific protein in complex samples based on the specific binding of antigens and antibodies. It is a novel immunobiochemical technique developed from gel electrophoresis and solid-phase immunoassay. Due to the high resolution of SDS-PAGE and the high specificity and sensitivity of solid-phase immunoassay, Western blotting has become a routine technique for protein analysis. It is commonly used to identify specific proteins and to perform qualitative and semi-quantitative analysis. Combined with chemiluminescence detection, it allows for simultaneous comparison of the expression levels of the same protein in multiple samples.
[0003] However, the following problems still exist in the current Western blotting experiments:
[0004] 1. After protein electrophoresis, collect the protein-gel mixture to prepare for subsequent membrane transfer. When there are many experimental samples, operators cannot process multiple gels simultaneously, which can easily lead to operational chaos.
[0005] Second, in the case of multiple batches of samples, manually completing the membrane transfer operation requires a high level of skill and expertise from the experimenter. Furthermore, the subsequent electroporation process needs to be carried out in an ice bath. Therefore, under certain laboratory conditions, prolonged exposure of samples to high temperatures can indirectly affect the stability of protein transfer to PVDF membranes (consumables can be equivalently replaced).
[0006] Therefore, there is an urgent need for a high-efficiency, multi-batch, and highly stable transfer box. Utility Model Content
[0007] To address the aforementioned technical problems, this utility model provides a transfer box, specifically a wet transfer box. Firstly, the transfer box contains multiple drawers, allowing for the transfer of up to four protein gels to the membrane at a time, facilitating multiple batch operations. Secondly, by introducing ice packs and transfer buffer inside the left and right side panels, the samples are kept at a low temperature before the actual electrophoresis, ensuring the stability of the transfer electrophoresis. Furthermore, the top and bottom plates of the transfer box are equipped with inlet and outlet valves, respectively, allowing a small amount of transfer buffer to be transferred as a liquid medium into the drawers, maintaining a low-temperature environment for transfer reagents and consumables, further improving the stability of the transfer electrophoresis. Even further, the top and bottom of the left and right side panels of the transfer box are equipped with opening structures and outlet valves, which improve the recovery efficiency of the transfer buffer.
[0008] The purpose of this invention is to enable the simultaneous transfer of up to four protein gels during Western blotting experiments. Furthermore, the two side plates can be freely placed in an ice box to control the transfer temperature, resulting in a more stable transfer process. Previously, transfer processes were typically performed in simple containers, making it impossible to control the transfer solution temperature. The order of the sponge, filter paper, gel, and transfer material was also manually controlled, leading to time-consuming processes and potential material loss during transfer. This new transfer box uses a device to fix the transfer position and utilizes an ice box to control the transfer environment, offering advantages such as short processing time, high efficiency, and ease of operation, thus ensuring the stability of subsequent electroblotting.
[0009] This utility model is achieved through the following technical solution:
[0010] This utility model provides a transfer box, including a box body with multiple drawers inside. The box body includes a top plate, a bottom plate, a rear side plate, and a left side plate and a right side plate for placing ice boxes and transfer solution. The top of the left side plate and the right side plate are open structures, the inside is hollow structures, and the bottom is provided with a drain valve.
[0011] Preferably, the plurality of drawer boxes consists of four drawer boxes.
[0012] Preferably, the four drawer boxes are designated as a first drawer box, a second drawer box, a third drawer box, and a fourth drawer box.
[0013] Preferably, each of the plurality of drawer boxes is equipped with a drawer inside, and each drawer has a groove inside for placing reagents and consumables for membrane transfer, and each drawer is externally connected to a pull bolt for controlling the extension and retraction of the drawer. The pull bolts are of the same size.
[0014] Preferably, the reagents and consumables for membrane transfer include a sponge, filter paper, protein gel, PVDF membrane, filter paper, and sponge placed in sequence.
[0015] Preferably, the drain valve includes a first drain valve and a second drain valve.
[0016] Preferably, the length, width, and height of the plurality of drawer boxes are all consistent.
[0017] Preferably, the left and right side plates are uniformly symmetrical; both the left and right side plates have a length direction and a width direction; and the left and right sides of the left or right side plates have different heights.
[0018] Preferably, the hollow structure is used to hold the ice box and transfer solution. The ice box and transfer solution are added to the left and right side plates through the opening structure, and the transfer solution is discharged and recycled through the drain valve. The ice box provides a low-temperature environment for the transfer solution and the transfer reagents and consumables in the drawer box.
[0019] Preferably, the top plate is equipped with an inlet valve, and the bottom plate is equipped with a third drain valve. A small amount of transfer solution is poured into the drawer box through the inlet valve and discharged and recycled through the drain valve. The small amount of transfer solution in the drawer box can further maintain a low-temperature environment for the reagents and consumables used in the transfer process.
[0020] Preferably, the drain valves are identical in size and function.
[0021] Preferably, when the drain valve is closed, the pre-transfer membrane begins; when the drain valve is opened, the transfer solution is recovered.
[0022] Compared with the prior art, the present invention has the following beneficial effects:
[0023] 1. This utility model includes two side panels, multiple drawer boxes, and an integrated pull-bolt device. After pulling out the drawer box, the reagents and consumables to be used for membrane transfer can be placed in the groove in a certain order. After pushing the drawer back, the transfer solution and ice pack can be added to the left and right side panels to provide a low-temperature environment for the transfer solution and the reagents and consumables in the drawer box. Furthermore, a small amount of transfer solution can be injected into the drawer box as a liquid medium through the inlet valve to maintain a low-temperature environment for the reagents and consumables used for membrane transfer, ensuring that the entire pre-transfer environment is at a certain low temperature for pre-transfer. When performing the actual electrophoresis transfer, the pre-cooled reagents and consumables can be taken out and transferred to the electrophoresis apparatus for protein-membrane transfer, which is beneficial to the stability of the reagents and consumables. Moreover, up to four gels can be transferred onto the PVDF membrane at the same time (the consumables can be equivalently replaced), which is beneficial for multiple batch operations.
[0024] 2. This utility model features an open structure at the top of the left and right side plates, which facilitates the addition of transfer solution and ice box; at the same time, a drain valve is provided at the bottom of the left and right side plates, which helps to improve the recovery efficiency of transfer solution.
[0025] 3. This invention transfers protein blots into a transfer chamber. Previously, transfer processes were typically performed in simple containers, making it impossible to control the transfer solution temperature. Furthermore, manually controlling the order of the sponge, filter paper, gel, and transfer material was time-consuming and prone to loss during the transfer process. In contrast, this invention uses a device to fix the transfer position and utilizes an ice pack to control the transfer environment, offering advantages such as short processing time, high efficiency, and ease of operation, thus ensuring the stability of subsequent electroporation. Attached Figure Description
[0026] Other features, objects, and advantages of the present invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:
[0027] Figure 1 This is a schematic diagram of the transfer membrane box of this utility model;
[0028] Figure 2This is a schematic diagram showing the left side panel and the ice box being installed in this utility model;
[0029] Figure 3 This is a schematic diagram showing the right side plate and the ice box being installed in this utility model;
[0030] Figure 4 This is a schematic diagram of the drawer box when closed in this utility model;
[0031] Figure 5 This is a schematic diagram of the drawer box in this utility model when it is pulled open;
[0032] In the diagram: 1. Transfer box; 2. Left side panel; 3. Right side panel; 4. First drawer box; 5. First drawer box; 6. First drawer box; 7. Third drain valve; 8. First drain valve; 9. Second drain valve; 10. Inlet valve; 11. Bottom plate; 12. Top plate; 13. Rear side panel; 14. Drawer; 15. Groove; 16. Pull bolt; 17. Detailed Implementation
[0033] The present invention will now be described in detail with reference to specific embodiments. These embodiments will help those skilled in the art to further understand the present invention, but do not limit the present invention in any way. It should be noted that those skilled in the art can make several changes and improvements without departing from the concept of the present invention. These all fall within the protection scope of the present invention.
[0034] This utility model provides a transfer box, such as Figure 1 As shown, the transfer box 1 includes a box body with four drawer boxes (first drawer box 4, second drawer box 5, third drawer box 6, and fourth drawer box 7) inside. The box body includes a top plate 13, a bottom plate 12, a rear side plate 14, and a left side plate 2 and a right side plate 3 for placing ice boxes and transfer liquid. The top of the left side plate and the right side plate are open structures, the inside is hollow structures, and the bottom is provided with a drain valve (first drain valve 9 and second drain valve 10).
[0035] The transfer reagents and consumables include a sponge, filter paper, protein gel, PVDF membrane, filter paper, and sponge placed in sequence.
[0036] The top plate is equipped with an inlet valve 11, and the bottom plate is equipped with a third drain valve 8. A small amount of transfer solution is poured into the drawer box through the inlet valve and discharged and recycled through the drain valve. The small amount of transfer solution in the drawer box can further maintain a low-temperature environment for the reagents and consumables used in the transfer process. The drain valves are identical in size and function; when the drain valve is closed, pre-transfer begins; when the drain valve is open, the transfer solution is recycled.
[0037] like Figure 2-3As shown, the ice box is placed within the hollow structure of the left and right side panels. The ice box and transfer solution are added to the left and right side panels through the openings, and the transfer solution is discharged and recycled through a drain valve. The ice box provides a low-temperature environment for the transfer solution and the transfer reagents and consumables in the drawer.
[0038] like Figure 4 As shown, each of the four drawer boxes is equipped with a drawer 15 inside. Each drawer has a groove 16 inside for placing reagents and consumables used in the transfer membrane process. Externally, each drawer is connected to a pull bolt 17 for controlling its extension and retraction. The length, width, and height of the four drawer boxes are all consistent. A schematic diagram of the drawer box when extended is shown below. Figure 5 As shown.
[0039] The left and right side panels are uniformly symmetrical; both the left and right side panels have a length direction and a width direction; the left and right sides of the left or right side panels have different heights.
[0040] This utility model is implemented in the following manner:
[0041] First, in transfer chamber 1, close the third drain valve 8, the first drain valve 9, and the second drain valve 10 on the bottom plate 12. Place the prepared ice pack into the left and right side plates 2 and 3. Transfer the prepared transfer solution into the left and right side plates. Transfer a small amount of transfer solution into drawer boxes 4-7 through the inlet valve 11 on the top plate 13. Then, pull the drawer boxes open and place the prepared sponge, filter paper, protein gel, PVDF membrane, filter paper, and sponge into the groove 16 of drawer 15 in sequence. The number of drawer boxes to open depends on the amount of protein gel. After loading, use the pull bolt 17 to push the drawer boxes back to preserve the reagents and consumables in the pre-cooled transfer solution.
[0042] During the formal electroporation, open the third drain valve 8, the first drain valve 9, and the second drain valve 10 to recover the transfer solution. Open drawer boxes 4 to 7 and take out the sponge-filter paper-glue-membrane-filter paper-sponge together for the formal electroporation.
[0043] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model. The scope of protection of this utility model is defined by the appended claims and their equivalents.
Claims
1. A transfer box, characterized in that, The box body includes a box with multiple drawers inside. The box body includes a top plate, a bottom plate, a rear side plate, and a left side plate and a right side plate for placing ice boxes and transfer solution. The top of the left side plate and the right side plate are open structures, the inside is hollow structures, and the bottom is equipped with a drain valve.
2. The transfer box according to claim 1, characterized in that, The plurality of drawer boxes refers to four drawer boxes.
3. The transfer box according to claim 2, characterized in that, The four drawer boxes are the first drawer box, the second drawer box, the third drawer box, and the fourth drawer box.
4. The transfer box according to claim 1, characterized in that, Each of the multiple drawer boxes is equipped with a drawer inside.
5. The transfer box according to claim 4, characterized in that, The drawers are all equipped with grooves inside for placing reagents and consumables for membrane transfer, and are all connected to bolts on the outside for controlling the extension and retraction of the drawers.
6. The transfer box according to claim 5, characterized in that, The transfer reagents and consumables include a sponge, filter paper, protein gel, PVDF membrane, filter paper, and sponge placed in sequence.
7. The transfer box according to claim 1, characterized in that, The hollow structure is used to hold the ice box and the transfer solution.
8. The transfer box according to claim 1, characterized in that, The drain valve includes a first drain valve and a second drain valve.
9. The transfer box according to claim 1, characterized in that, The top plate is equipped with a liquid inlet valve.
10. The transfer box according to claim 1, characterized in that, The base plate is equipped with a third drain valve.