Microbial culture apparatus sealing structure and microbial culture apparatus

Abstract

The utility model belongs to the technical field of microorganism culture equipment, and particularly relates to a microorganism culture equipment sealing structure and microorganism culture equipment. The sealing structure of the non-fiber breathable sealing replaceable membrane comprises a microorganism culture device, a gasket, the membrane and a cover body which are matched with one another, wherein the membrane and the gasket are arranged between a bottle opening of the microorganism culture device and the cover body; the microbe culture apparatus is made of high borosilicate glass, the gasket is made of expanded polytetrafluoroethylene (ePTFE), the membrane is made of expanded polytetrafluoroethylene (ePTFE) (a composite membrane with a support), and the cover body is made of polybutylene terephthalate (PBT). According to the utility model, through the design of the bottleneck structure of the microbial culture apparatus and the material selection of the microbial culture apparatus, the gasket, the diaphragm and the cover body, the microbial culture apparatus which meets the requirements of microbial culture performance and is lower in cost is constructed, and the microbial culture apparatus has a very good application prospect.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of microorganism culture equipment, and particularly relates to a microorganism culture equipment sealing structure and microorganism culture equipment. BACKGROUND

[0002] Triangular flask floating film culture is a technique for microorganism culture, especially suitable for suspension culture and co-culture of adherent microorganisms. This culture method uses a triangular flask as the culture container, which contains culture medium and microorganisms. The microorganisms form a floating film structure in the flask. The floating film is a thin layer formed by the aggregation of microorganisms, which can promote the interaction between microorganisms and is beneficial to the growth and differentiation of microorganisms. Triangular flask floating film culture has the advantages of simple operation and low cost, and is suitable for large-scale microorganism culture, drug screening and production of biological products. By adjusting the culture conditions such as temperature, pH value, oxygen and carbon dioxide concentration, the growth environment of microorganisms can be optimized, and the efficiency and yield of microorganism culture can be improved.

[0003] When aerobic bacteria or facultative bacteria are cultured by triangular flask floating film culture, materials are generally used to cover the bottle opening of the microorganism culture equipment to prevent the entry of dust and bacteria, which may affect the culture. The current materials used to cover the bottle opening of the microorganism culture equipment are generally hard silicone, plant fiber products or glass, which are tightly sealed by elastic force or gravity to achieve the purpose. The filtration effect of fiber or the goose neck structure is generally used to isolate dust or bacteria. However, these existing materials for covering the bottle opening of the microorganism culture equipment still have problems in use, such as: the hard silicone plug has the problem of poor high pressure effect, high pressure sterilization requires a gas permeation hole or separate high pressure, otherwise the bottle may be burst or the plug may be pressed into the bottle due to the pressure difference between the inside and outside, and the plug needs to be checked regularly for air tightness and replaced, which has the problem of high cost; the plant fiber plug has the risk of product contamination due to fiber shedding, and has poor high pressure resistance; the glass plug is generally placed by gravity, the bottle opening of the microorganism culture equipment is not tight, and has the problems of heavy weight, difficult operation and easy breakage.

[0004] Therefore, there is an urgent need in the art for new microorganism culture equipment to optimize the structure and material selection of the microorganism culture equipment. SUMMARY

[0005] In view of the problems of the prior art, the present application provides a microorganism culture equipment containing a fiber-free air-permeable sealing replaceable membrane.

[0006] The utility model provides a kind of microbial culture equipment sealing structure containing air-permeable sealing replaceable membrane, including mutually matched microbial culture equipment bottle mouth and cover, gasket and membrane are sequentially arranged between the microbial culture equipment bottle mouth and cover;The material of the microbial culture equipment bottle mouth is high borosilicate glass, the material of the cover is polybutylene terephthalate, the material of the membrane is expanded polytetrafluoroethylene, and the material of the gasket is expanded polytetrafluoroethylene.

[0007] Preferably, the microbial culture equipment bottle mouth and cover are matched by threads.

[0008] Preferably, the length of the thread is 1.5 turns.

[0009] Preferably, the thickness of the membrane is 220±60 μm.

[0010] Preferably, the membrane is a composite membrane with support.

[0011] Preferably, the membrane is attached with an autoclave indicator.

[0012] Preferably, the membrane is selected from medical expanded polytetrafluoroethylene (ePTFE) liquid-blocking air-permeable membrane.

[0013] Preferably, the inner diameter of the microbial culture equipment bottle mouth is 40±20 mm.

[0014] The utility model also provides a kind of microbial culture equipment containing air-permeable sealing replaceable membrane, including microbial culture equipment bottle body and the above-mentioned microbial culture equipment sealing structure containing air-permeable sealing replaceable membrane.

[0015] The utility model optimizes the structure of microbial culture equipment and the selection of its material, and achieves the following beneficial technical effects:

[0016] 1. Non-fibrous: according to the latest requirements of cGMP, it is required to avoid fiber dust as much as possible during cultivation. The utility model does not produce fiber dust, which meets the cGMP standard.

[0017] 2. Low cost: high borosilicate triangular glass bottles can be reused for at least 10 years. The cover and gasket can be subjected to online steam sterilization at 145°C for 30 minutes, a total of 150 times. They can also be subjected to high-pressure steam sterilization at 130°C for 30 minutes, a total of 400 times. The membrane is disposable, and the cost is less than 2 yuan per piece. Therefore, the overall use cost of the utility model is low.

[0018] 3. Easy sterilization: the whole system theory can be sterilized integrally, which can adapt to ethylene oxide (ETO) sterilization, steam sterilization, and a certain degree of Gamma and E-beam sterilization mode, can be 145°C online steam sterilization for 30 minutes, cumulative 150 times, 130°C high pressure steam sterilization for 30 minutes, cumulative 400 times;

[0019] 4. Easy fastening and convenient operation: the bottle opening thread of the microbiological culture equipment is half a circle, the bottle plug can be quickly taken off, the gasket does not adhere to the microbiological culture equipment bottle opening, and the pollution risk is reduced;

[0020] 5. Visibility: the membrane is provided with a high-pressure sterilization indicator, which can effectively indicate the completeness of high-pressure sterilization;

[0021] 6. Hydrophobicity: the membrane is a hydrophobic material, and the system can pass various verifications (bacterial retention test, hot air resistance test, oxidizing agent resistance test, leakage test, resistance test, 132°C online steam and high pressure steam resistance, online steam sterilization, extractable test of water and methanol as extraction liquid, extractable test of pure water as extraction liquid, chemical compatibility, no fiber release, endotoxin test, in vitro biological safety, and in vivo biological safety) through the hydrophobic material;

[0022] 7. Sealing property: the system can pass the sealing property verification;

[0023] 8. Air permeability: in the preferred scheme, the membrane is selected from a medical ePTFE liquid-blocking air-permeable membrane, the filtering precision of the membrane is 0.2 or 0.22 um, the membrane can effectively block the pollution of bacteria and particulate matter while blocking liquid, and the smooth in and out of airflow can be maintained. The air permeation amount is 40.00 s@100 ml*1ln2*4.88in.water;

[0024] 9. Easy storage: the triangular bottle of the utility model can be used for 5 years under normal temperature, shade and standard packaging.

[0025] Obviously, according to the above content of the utility model, according to the ordinary technical knowledge and conventional means in the art, other various forms of modifications, replacements or changes can be made without departing from the above basic technical idea of the utility model.

[0026] The above content of the utility model is further explained in detail through the specific implementation mode in the form of examples. However, this should not be understood as the range of the above subject matter of the utility model being limited to the following examples. All the technologies realized based on the above content of the utility model belong to the range of the utility model. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 It is a structural schematic view of the utility model.

[0028] Among them, 1-the mouth of the microbial culture equipment bottle, 2-the gasket, 3-the membrane, 4-the cap, and 5-the body of the microbial culture equipment bottle. Detailed Implementation

[0029] Example 1: Microbial culture equipment for microbial culture

[0030] This embodiment provides a microbial culture device containing a fiber-free, breathable, and replaceable membrane.

[0031] like Figure 1 As shown, the sealing structure of the fiber-free, breathable, and replaceable diaphragm includes a microbial culture instrument bottle mouth 1, a gasket, a diaphragm, and a cap that fit together. The microbial culture instrument bottle mouth 1 and cap 4 are threaded together, with a thread length of 1.5 turns. A gasket 2 and a diaphragm 3 are sequentially arranged between the microbial culture instrument bottle mouth 1 and cap 4, with the diaphragm 3 tightly attached above the gasket 2. The microbial culture instrument is made of borosilicate glass. The inner diameter of the microbial culture instrument bottle mouth 1 is 40±20 mm. The gasket 2 is made of expanded polytetrafluoroethylene (ePTFE). The cap 4 is made of polybutylene terephthalate (PBT). The diaphragm 3 is made of expanded polytetrafluoroethylene (ePTFE) (a supported composite membrane) with a thickness of 220±60 μm. An autoclave indicator is attached to the diaphragm 3. The membrane 3 in this embodiment is disposable. Membrane 3 should pass the 107 cfu / cm² defective shortwave monocytogenes (ATCC*19146) retention test according to ASTM F838, meet the "no fiber release" standard defined in FDA 21 CFR 210.3 (b) (6), and can be sterilized online at 145°C for 30 min for a total of 150 times, or autoclaved at 130°C for 30 min for a total of 400 times. The membrane is very clean, with ultra-low extractable / leached substances, and has excellent resistance to corrosion, oxidation and organic solvents. It has good chemical compatibility, pH=1-14, and temperature resistance up to 180°C.

[0032] Furthermore, this embodiment also provides a microbial culture device with the above-mentioned sealing structure, including a microbial culture device bottle 5 and the above-mentioned disposable fiber-free microbial culture device bottle mouth breathable sealing structure. The microbial culture device bottle 5 can be made of borosilicate glass or other materials.

[0033] This invention, through the design of the bottle mouth structure of the microbial culture equipment and the selection of materials for the microbial culture equipment, gaskets, membranes, and caps, constructs a microbial culture equipment that meets the performance requirements of microbial culture at a lower cost, and has a promising application prospect.

Claims

1. A seal for microbiological culture apparatus, characterised in that: The application relates to a microbial culture equipment bottle mouth (1) and a cover (4) matched with each other, a gasket (2) and a film (3) being arranged between the microbial culture equipment bottle mouth (1) and the cover (4) in sequence; the material of the microbial culture equipment bottle mouth (1) is high-boron-silicon glass, the material of the cover (4) is polybutylene terephthalate, the material of the film (3) is expanded polytetrafluoroethylene, and the material of the gasket (2) is expanded polytetrafluoroethylene.

2. The microorganism culture apparatus seal structure according to claim 1, characterized by: The microbial culture equipment bottle mouth (1) and the cover (4) are matched through threads.

3. The microorganism culture apparatus seal structure according to claim 2, characterized by: The length of the thread is 1.5 turns.

4. The microorganism culture apparatus seal structure according to claim 1, characterized by: The thickness of the film (3) is 220+ / -60 mu m.

5. The microorganism culture apparatus seal structure according to claim 1, characterized by: The film (3) is a composite film with support.

6. The microorganism culture apparatus seal structure according to claim 1, characterized by: An autoclaving indicator is attached to the film (3).

7. The microorganism culture apparatus seal structure according to claim 1, characterized by: The film (3) is selected from medical expanded polytetrafluoroethylene liquid-blocking and air-permeable films.

8. The microorganism culture apparatus seal structure according to claim 1, characterized by: The inner diameter of the microbial culture equipment bottle mouth (1) is 40+ / -20 mm.

9. A microbiological culture apparatus, characterized by: The application further relates to a microbial culture equipment bottle body (5) and the microbial culture equipment sealing structure according to any one of claims 1-8.

Images