Cell culture pore plate capable of vertically or horizontally placing cell slide
By introducing slots and step structures into the cell culture plate, the cell slides can be placed vertically or horizontally, solving the problem of difficult retrieval in the prior art and achieving safe sample retrieval and resource conservation.
Patent Information
- Application Number
- CN202423160547.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-20
- Publication Date
- 2025-12-05
- Estimated Expiration
- 2034-12-20
AI Technical Summary
Existing cell slides tend to stick to the bottom of cell culture plates when placed, making them difficult to remove, resulting in sample damage and wasted resources.
Design a cell culture plate with slots and a stepped structure to allow cell slides to be placed vertically or horizontally, ensuring a distance from the bottom of the plate for easy removal.
This method enables convenient removal of cell slides, avoiding sample damage and resource waste, and improving experimental efficiency.
Smart Images
Figure CN223633385U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to cell culture well plate technical field, and specifically relates to a cell culture well plate capable of vertically or horizontally placing cell climbing sheet. BACKGROUND
[0002] It is a common technology in current biological medical research to observe bacteria and cell morphology by using a scanning electron microscope. When observing bacteria and cell morphology by using the scanning electron microscope, the bacteria or cell samples are usually prepared into bacteria or cell climbing sheets, and then special treatment is needed to observe under the scanning electron microscope. Taking plaque biofilm as an example, the sample preparation method is as follows: first, washing twice with PBS, then fixing for 4 hours at 4 DEG C with glutaraldehyde electron microscope preservative, then washing three times with PBS, and then dehydrating with 50%, 70%, 90%, 99% and 100% ethanol molecular sieves in sequence. After the biofilm sample is completely dried, the sample is gold-plated.
[0003] The existing cell climbing sheet is usually placed at the bottom of the cell culture well plate and often adheres to the bottom of the cell culture well plate. This cell climbing sheet placement method usually has the following disadvantages: it is not easy to take out; when scanning the electron microscope, the cell climbing sheet needs to be clamped with tweezers, which often damages the sample, causing waste of resources and funds. UTILITY MODEL CONTENT
[0004] The utility model aims at providing a cell culture well plate capable of vertically or horizontally placing cell climbing sheet, which is used to solve the technical problems in the background art.
[0005] To achieve the above-mentioned purpose, the utility model adopts the following technical scheme:
[0006] A cell culture well plate capable of vertically or horizontally placing cell climbing sheet, comprising a cell culture well plate body, at least one first cell culture well is arranged on the cell culture well plate body, at least one pair of clamping grooves for vertically placing cell climbing sheet is arranged at the hole edge of the first cell culture well, a second cell culture well in communication with the first cell culture well is arranged at the hole bottom of the first cell culture well, and a step for horizontally placing cell climbing sheet is formed between the hole bottom of the first cell culture well and the hole bottom of the second cell culture well.
[0007] Further, the shape of the cell culture well plate body is a circle, a square, a rectangle or any other regular polygon with an even number of sides.
[0008] Further, the shape of the first cell culture well is a circle, a square, a rectangle or any other regular polygon with an even number of sides.
[0009] Further, the shape of the second cell culture well is a circle, a square, a rectangle or any other regular polygon with an even number of sides.
[0010] Further, the shape of the cell climbing sheet is circular, square, rectangular or any other regular polygon with even number of sides.
[0011] Further, the depth of the first cell culture well is greater than or equal to the height of the cell climbing sheet placed vertically.
[0012] Further, the depth of the first cell culture well is greater than or equal to the thickness of the cell climbing sheet placed horizontally.
[0013] Compared with the prior art, the cell climbing sheet can be placed vertically or horizontally, and there is a certain distance between the cell climbing sheet and the bottom of the cell culture well plate when the cell climbing sheet is placed vertically or horizontally, so that the cell climbing sheet is convenient to take, and sample damage and resource and fund waste are avoided. BRIEF DESCRIPTION OF DRAWINGS
[0014] In order to more clearly illustrate the technical solutions in the embodiments, the following will briefly introduce the drawings needed to be used in the embodiment description. Obviously, the drawings described below are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor on the basis of these drawings.
[0015] Figure 1 is an embodiment of the cell culture well plate of the present application;
[0016] Figure 2 is a partial sectional view of Figure 1 ;
[0017] Figure 3 is a top view of Figure 1 ;
[0018] Figure 4 is an A-A sectional view of Figure 3 ;
[0019] Figure 5 is a schematic view of the cell climbing sheet placed vertically in the cell culture well plate of the embodiment; Figure 1
[0020] Figure 6 is a partial sectional view of Figure 5 ;
[0021] Figure 7 is a top view of Figure 5 ;
[0022] Figure 8 is a B-B sectional view of Figure 7 ;
[0023] Figure 9 is a schematic view of a cell culture plate according to an embodiment of the present application with a cell culture insert horizontally placed in the cell culture plate; Figure 1 is a schematic view of a cell culture plate according to an embodiment of the present application with a cell culture insert horizontally placed in the cell culture plate;
[0024] Figure 10 is a partial cross-sectional view of a cell culture plate according to an embodiment of the present application; Figure 9 is a partial cross-sectional view of a cell culture plate according to an embodiment of the present application;
[0025] Figure 11 is a top view of a cell culture plate according to an embodiment of the present application; Figure 9 is a top view of a cell culture plate according to an embodiment of the present application;
[0026] Figure 12 is a cross-sectional view of a cell culture plate according to an embodiment of the present application; Figure 11 is a cross-sectional view of a cell culture plate according to an embodiment of the present application;
[0027] Figures 13-21 are further embodiments of the cell culture plate according to the present application;
[0028] BRIEF DESCRIPTION OF DRAWINGS 1. Cell culture plate body; 2. First cell culture well; 3. Clamping groove; 4. Second cell culture well; 5. Step; 6. Cell culture insert. DETAILED DESCRIPTION
[0029] In order to make the objectives, technical solutions and advantages of the present application clearer, the following will describe the technical solutions in the embodiments of the present application clearly and completely with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. The components of the embodiments of the present application described and shown in the drawings can be arranged and designed in various different configurations.
[0030] Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the claimed present application, but only represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor fall within the scope of protection of the present application.
[0031] In the description of the embodiments of the utility model, it needs to be explained that, if the terms "upper", "lower", "horizontal", "inner" and the like indicate the orientation or position relationship based on the orientation or position relationship shown in the drawings, or the orientation or position relationship of the product of the application when it is usually placed, it is only for the convenience of describing the utility model and simplifying the description, and it does not indicate or imply that the device or element indicated must have a specific orientation, a specific orientation and operation, therefore it cannot be understood as a limitation on the utility model. In addition, the terms "first", "second" and the like are only used for differentiation, and cannot be understood as indicating or implying relative importance. In addition, if the term "horizontal" appears, it does not mean that the component must be absolutely horizontal, but can be slightly inclined. For example, "horizontal" only means that its direction is relatively more horizontal than "vertical", and does not mean that the structure must be completely horizontal, but can be slightly inclined.
[0032] In the description of the embodiments of the utility model, it also needs to be explained that, unless otherwise explicitly specified and limited, if the terms "set", "install", "connect", "connect" appear, they should be understood in a broad sense, for example, they can be fixedly connected, or can be detachably connected, or integrally connected; can be mechanically connected, or can be electrically connected; can be directly connected, or indirectly connected through an intermediate medium, or can be connected inside two elements. For ordinary skilled persons in the art, the specific meaning of the above terms in the utility model can be understood according to the specific situation.
[0033] The utility model will be further described below in combination with the drawings:
[0034] Referring to Figures 1-12 The utility model embodiment provides a cell culture well plate capable of vertically or horizontally placing cell climbing pieces, comprising a cell culture well plate body 1, six first cell culture wells 2 are arranged on the cell culture well plate body 1, a pair of clamping grooves 3 for vertically placing cell climbing pieces 6 is formed at the hole edge of each first cell culture well 2, and each pair of clamping grooves 3 is communicated with the corresponding first cell culture well 2, a second cell culture well 4 communicated with the first cell culture well 2 is formed at the hole bottom of each first cell culture well 2, and a step 5 for horizontally placing cell climbing pieces 6 is formed between the hole bottom of each first cell culture well 2 and the hole bottom of the corresponding second cell culture well 4.
[0035] Specifically, in the above embodiment, the shape of the cell culture well plate body 1 is rectangular, the shape of the first cell culture well 2 and the second cell culture well 4 is circular, the shape of the vertically placed cell climbing piece 6 is rectangular, and the shape of the horizontally placed cell climbing piece 6 is circular.
[0036] Specifically, in the above embodiment, the hole depth of the first cell culture well 2 is equal to the height of the vertically placed cell climbing piece 5.
[0037] Specifically, in the above embodiments, the first cell culture well 2 has a well depth greater than the thickness of the horizontally placed cell culture slide 5.
[0038] It can be understood that the number of the first cell culture wells 2 and the second cell culture wells 4 provided on the cell culture well plate body 1 can be increased or decreased according to actual needs. In the prior art, conventional cell culture well plates for biological experiments have three specifications of 6 wells, 12 wells and 24 wells. However, the cell culture well plate provided by the present application is not limited to the three specifications.
[0039] Similarly, it can be understood that the number of the clamping grooves 3 is not limited to one pair as shown in the figure, and can be multiple pairs. The number of pairs can be set according to actual needs.
[0040] In other embodiments, referring to Figures 13-21 , the shape of the cell culture well plate body 1 can be circular, square or any other regular polygon with an even number of sides, such as regular hexagonal, regular octagonal,..., regular N-sided polygon; N is an even number greater than 4.
[0041] In other embodiments, referring to Figures 13-21 , the shape of the first cell culture well 2 can be square, rectangular or any other regular polygon with an even number of sides, such as regular hexagonal, regular octagonal,..., regular N-sided polygon; N is an even number greater than 4.
[0042] In other embodiments, referring to Figures 13-21 , the shape of the second cell culture well 4 can also be square, rectangular or any other regular polygon with an even number of sides, such as regular hexagonal, regular octagonal,..., regular N-sided polygon; N is an even number greater than 4.
[0043] In other embodiments, the shape of the cell culture slide 6 can be square, rectangular or any other regular polygon with an even number of sides, such as regular hexagonal, regular octagonal,..., regular N-sided polygon; N is an even number greater than 4.
[0044] In other embodiments, the well depth of the first cell culture well 2 can be greater than the height of the vertically placed cell culture slide 5.
[0045] In other embodiments, the well depth of the first cell culture well 2 can be equal to the thickness of the horizontally placed cell culture slide 5.
[0046] The use principle of the cell culture well plate provided by the present application, which can vertically or horizontally place a cell culture slide, is as follows:
[0047] In use, the cell climbing sheet 6 can be placed in a vertical manner according to actual needs through the at least one pair of clamping grooves 3 opened at the edge of the first cell culture hole 2, or placed in a horizontal manner through the step 5 formed between the hole bottom of the first cell culture hole 2 and the hole bottom of the second cell culture hole 4; no matter whether the cell climbing sheet 6 is placed in a vertical manner or a horizontal manner, there is a certain distance between the cell climbing sheet 6 and the bottom of the cell culture hole plate body 1, which can prevent the two from being attached, facilitate taking out the cell climbing sheet 6 from the cell culture hole plate body 1, can well meet the experimental requirements, and avoid damage of the cell sample and waste of resources and funds.
[0048] Finally, it should be pointed out that the above description is only an embodiment of the present application, and does not limit the patent range of the present application, and any equivalent structure or equivalent process transformation obtained by using the content of the present application specification and drawings, or direct or indirect application in other related technical fields, are also included in the patent protection range of the present application.
Claims
1. A cell culture well plate which can be placed vertically or horizontally with cell climbing sheets, comprising a cell culture well plate body (1), characterized in that: The cell culture well plate body (1) is provided with at least one first cell culture well (2), at least one pair of clamping grooves (3) for vertically placing cell climbing sheets (6) is arranged at the well edge of the first cell culture well (2), a second cell culture well (4) is arranged at the well bottom of the first cell culture well (2) and communicates with the first cell culture well (2), and a step (5) for horizontally placing cell climbing sheets (6) is formed between the well bottom of the first cell culture well (2) and the well bottom of the second cell culture well (4).
2. The cell culture well plate with vertically or horizontally placeable cell-crawl slides of claim 1, wherein: The cell culture well plate body (1) is circular, square, rectangular or any other regular polygon with an even number of sides.
3. The cell culture well plate that can be placed vertically or horizontally cell climbing slices of claim 1, characterized by: The first cell culture well (2) is circular, square, rectangular or any other regular polygon with an even number of sides.
4. The cell culture well plate that can be placed vertically or horizontally cell climbing slices of claim 1, wherein: The second cell culture well (4) is circular, square, rectangular or any other regular polygon with an even number of sides.
5. The cell culture well plate capable of vertically or horizontally placed cell-crawl slides according to claim 1, wherein: The cell climbing sheet (6) is circular, square, rectangular or any other regular polygon with an even number of sides.
6. The cell culture well plate that can be placed vertically or horizontally cell climbing slices of claim 1, wherein: The depth of the first cell culture well (2) is greater than or equal to the height of the vertically placed cell climbing sheet (6).
7. The cell culture well plate capable of vertically or horizontally placed cell-crawl slides according to claim 1, wherein: The depth of the first cell culture well (2) is greater than or equal to the thickness of the horizontally placed cell climbing sheet (6).