Portable nucleic acid detection equipment

Abstract

The utility model discloses portable nucleic acid detection equipment which comprises a mounting cylinder, a swinging mechanism is fixedly mounted in the mounting cylinder, a nucleic acid reaction tube can be inserted into the swinging mechanism, and a sealing cover can be inserted into the upper surface of the swinging mechanism, so that the nucleic acid reaction tube can be sealed in the swinging mechanism by the sealing cover. Through the design of the swinging mechanism, the position consistency of a to-be-detected sample in the nucleic acid reaction tube is further ensured through a heating mode combining rotation and swinging, the sample can be more uniformly heated in the nucleic acid reaction tube, and compared with pure static heating, the speed of the sample reaching a proper reaction temperature can be increased, so that the reaction efficiency is improved. The sample pretreatment efficiency in the nucleic acid detection process is improved.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid detection technology, specifically a portable nucleic acid detection device. Background Technology

[0002] The ability to quickly and efficiently detect viruses, thereby enabling timely discovery and prevention of viral infections, is of great significance to epidemic prevention and control.

[0003] For example, the national authorized patent announcement number CN218860746U discloses a portable nucleic acid detection device and system. The device includes a main body; the main body is provided with an installation cavity for mounting a carrier module, the carrier module is used to accommodate a nucleic acid reaction tube, and the nucleic acid reaction tube is used to accommodate the analyte; the carrier module is connected to a heating module, which is used to adjust the temperature of the carrier module to adjust the temperature of the nucleic acid reaction tube; the main body is connected to a light-emitting module, which is located at the bottom of the carrier module, and the carrier module has a light-transmitting hole facing the light-emitting module so that the emitted light from the light-emitting module can irradiate the nucleic acid reaction tube through the light-transmitting hole; the main body is also provided with an observation window, and a first observation hole is provided on the first side of the carrier module, which is used to observe the nucleic acid reaction tube; when the carrier module is installed in the installation cavity, the observation window is used to observe the nucleic acid reaction tube through the first observation hole.

[0004] However, in the aforementioned portable nucleic acid testing equipment and systems, the analytes in the nucleic acid reaction tubes cannot maintain a consistent position after filling. This leads to different degrees of contact between the analytes and the heating and light-emitting modules in different nucleic acid reaction tubes, thus affecting the temperature and light uniformity of the reaction. This difference results in different reaction conditions in different nucleic acid reaction tubes, leading to inconsistent test results. Utility Model Content

[0005] The purpose of this invention is to provide a portable nucleic acid detection device to solve the problem mentioned in the background art that the analyte in the nucleic acid reaction tube cannot maintain a consistent position after filling, which leads to different degrees of contact between the analyte and the heating module, light-emitting module, etc. in different nucleic acid reaction tubes, resulting in inconsistent detection results.

[0006] To achieve the above objectives, this utility model provides the following technical solution:

[0007] A portable nucleic acid testing device includes: an installation cylinder, a swinging mechanism fixedly installed inside the installation cylinder, a nucleic acid reaction tube that can be inserted into the swinging mechanism, and a sealing cap that can be inserted into the upper surface of the swinging mechanism to seal the nucleic acid reaction tube inside the swinging mechanism.

[0008] Preferably, the upper surface of the mounting cylinder is provided with an annular groove, so that the bottom of the nucleic acid reaction tube inserted into the swinging mechanism can penetrate into the annular groove. A heating ring is provided on the upper surface of the annular groove, so that the heating ring can be flush with the bottom of the nucleic acid reaction tube for heating.

[0009] Preferably, a tube lens is inserted into the outer surface of the mounting tube, so that the other end of the tube lens can be inserted into the annular groove, and the tube lens can be aligned with the bottom of the nucleic acid reaction tube. An LED bead is fixedly installed at one end of the annular groove, and the LED bead can be aligned with and illuminate the nucleic acid reaction tube that penetrates into the annular groove, so that the nucleic acid reaction tube that penetrates into the annular groove is located between the LED bead and the tube lens.

[0010] Preferably, the outer surface of the LED bead is fitted with a heat-insulating window.

[0011] Preferably, the swirling mechanism includes a motor, which is fixedly installed inside the mounting cylinder. A swirling disc is fixedly installed on the upper surface of the motor's output shaft, causing the swirling disc to rotate and cover the upper surface of the annular groove. An insertion port is provided on the upper surface of the swirling disc, which is connected to the annular groove. A nucleic acid reaction tube can be inserted into the insertion port, allowing the bottom of the nucleic acid reaction tube to penetrate into the annular groove.

[0012] Preferably, the outer surface of the swivel disc is provided with digital markings and anti-slip strips, and each set of digital markings is flush with each set of insertion ports.

[0013] Compared with the prior art, the beneficial effects of this utility model are:

[0014] 1. Through the design of the microscope, ring groove, LED beads, heating ring, and swinging mechanism, during nucleic acid detection, the sample to be tested is placed in the nucleic acid reaction tube, and then the nucleic acid reaction tube is inserted into the swinging mechanism, with the bottom of the nucleic acid reaction tube inserted into the ring groove. Simultaneously, the heating ring in the ring groove heats the bottom of the nucleic acid reaction tube within the ring groove. While heating, the swinging mechanism rotates the nucleic acid reaction tube, causing it to be heated and rotated simultaneously above the heating ring. This rotating and swinging motion causes the sample to be tested to be thrown to the bottom of the sample tube. This ensures the consistency of the sample's position within the nucleic acid reaction tube, allowing for uniform heating of the analytes within the tube. This results in more uniform reaction conditions for the target substances, such as nucleic acids, in the sample during subsequent nucleic acid detection. This promotes the reaction of the nucleic acid with other reagents until the set heating time is reached. Then, the LED lights illuminate the nucleic acid reaction tube within the ring groove, allowing staff to observe the fluorescence changes through a microscope to determine if a specific reaction has occurred. Based on the fluorescence reaction, the presence of the target nucleic acid sequence in the sample can be determined, leading to the detection result.

[0015] 2. Through the design of the motor, spinning disc, insertion port, and digital labeling, during nucleic acid testing, the sample to be tested is placed in the nucleic acid reaction tube, which is then inserted into the insertion port of the spinning disc, with the bottom of the nucleic acid reaction tube penetrating into the ring groove. Simultaneously, the heating ring within the ring groove heats the bottom of the nucleic acid reaction tube. While heating, the motor drives the spinning disc to rotate, causing the inserted nucleic acid reaction tube to rotate and spin. This rotation and spinning motion, with the nucleic acid reaction tube being heated above the heating ring, throws the sample to the bottom of the sample tube, thus ensuring... To ensure the consistency of the position of the sample to be tested in the nucleic acid reaction tube, and by combining rotation and shaking with heating, the sample can be heated more evenly in the nucleic acid reaction tube. Compared with simple static heating, it can speed up the process of the sample reaching the appropriate reaction temperature and improve the efficiency of sample pretreatment in the nucleic acid detection process. Then, the staff can observe the changes in fluorescence in the nucleic acid reaction tube through the tube lens. After the current nucleic acid reaction tube has been observed, the staff can record the nucleic acid reaction tube number after testing according to the number marking on the rotating plate. Then, the rotating plate can be rotated to bring another set of nucleic acid reaction tubes level with the LED beads in the ring groove, so that the staff can observe each set of nucleic acid reaction tubes individually through the tube lens. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of the portable nucleic acid testing device of this utility model;

[0017] Figure 2 This is a schematic diagram of the structure of the tube mirror of this utility model;

[0018] Figure 3 This is a schematic diagram of the structure of the annular groove and heating ring of this utility model;

[0019] Figure 4 This is a schematic diagram of the swinging mechanism of this utility model;

[0020] Figure 5 This is a schematic diagram of the structure of the LED lamp beads and the heat insulation window of this utility model;

[0021] Figure 6 This is a schematic diagram of the structure of the cylindrical lens of this utility model being inserted into the annular groove.

[0022] In the diagram: 1. Mounting cylinder; 101. Sealed cover; 102. Cylindrical lens; 103. LED light bead; 104. Circular groove; 105. Heating ring; 106. Heat insulation window; 2. Swinging mechanism; 201. Motor; 202. Swinging disc; 203. Insertion port; 204. Anti-slip strip; 205. Digital label. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Please see Figures 1-6 This embodiment provides the following technical solution:

[0025] like Figures 1-2 As shown, a portable nucleic acid testing device includes: an installation cylinder 1, a swinging mechanism 2 fixedly installed inside the installation cylinder 1, a nucleic acid reaction tube that can be inserted into the swinging mechanism 2, and a sealing cap 101 that can be inserted into the upper surface of the swinging mechanism 2 so that the sealing cap 101 can seal the nucleic acid reaction tube inside the swinging mechanism 2.

[0026] An annular groove 104 is provided on the upper surface of the mounting cylinder 1, so that the bottom of the nucleic acid reaction tube inserted into the swing mechanism 2 can penetrate into the annular groove 104. A heating ring 105 is provided on the upper surface of the annular groove 104, so that the heating ring 105 can be flush with the bottom of the nucleic acid reaction tube for heating.

[0027] A microscope 102 is inserted into the outer surface of the mounting tube 1, so that the other end of the microscope 102 can be inserted into the annular groove 104, so that the microscope 102 is opposite to the bottom of the nucleic acid reaction tube. An LED bead 103 is fixedly installed at one end of the annular groove 104. The LED bead 103 is opposite to and illuminates the nucleic acid reaction tube that penetrates into the annular groove 104, so that the nucleic acid reaction tube that penetrates into the annular groove 104 is located between the LED bead 103 and the microscope 102.

[0028] The outer surface of the LED lamp bead 103 is fitted with a heat insulation window 106.

[0029] Through the design of the tube lens 102, the annular groove 104, the LED beads 103, the heating ring 105, and the swinging mechanism 2, during nucleic acid detection, the sample to be tested is placed in the nucleic acid reaction tube, and then the nucleic acid reaction tube is inserted into the swinging mechanism 2, with the bottom of the nucleic acid reaction tube inserted into the annular groove 104. Simultaneously, the heating ring 105 in the annular groove 104 is activated to heat the bottom of the nucleic acid reaction tube within the annular groove 104. While heating, the swinging mechanism 2 drives the nucleic acid reaction tube to rotate and swing, thus allowing the nucleic acid reaction tube to be heated and rotated simultaneously at the upper end of the heating ring 105. The rotating and swinging nucleic acid reaction tube can then shake the sample to... The bottom of the sample tube ensures the consistency of the position of the sample in the nucleic acid reaction tube, which in turn ensures that the analytes in the nucleic acid reaction tube are heated to the same degree. This makes the reaction conditions of the target substances such as nucleic acids in the sample more uniform in the subsequent nucleic acid detection reaction, promoting the reaction of nucleic acids in the nucleic acid reaction tube with other reagents. After the set heating time is reached, the LED light bead 103 is activated to irradiate the nucleic acid reaction tube in the annular groove 104. The staff can then observe the changes in fluorescence in the nucleic acid reaction tube through the tube lens 102 to determine whether a specific reaction has occurred. Based on the fluorescence reaction, the presence of the target nucleic acid sequence in the sample can be determined, thereby obtaining the detection result.

[0030] like Figures 3-6 As shown, the swishing mechanism 2 includes a motor 201, which is fixedly installed inside the mounting cylinder 1. A swishing disc 202 is fixedly installed on the upper surface of the output shaft of the motor 201, so that the swishing disc 202 rotates and covers the upper surface of the annular groove 104. An insertion port 203 is provided on the upper surface of the swishing disc 202, which is connected to the annular groove 104. A nucleic acid reaction tube can be inserted into the insertion port 203, so that the bottom of the nucleic acid reaction tube can penetrate into the annular groove 104.

[0031] The outer surface of the swivel plate 202 is provided with number markings 205 and anti-slip strips 204, and each set of number markings 205 is flush with each set of insertion ports 203.

[0032] Through the design of the motor 201, the spinning disc 202, the insertion port 203, and the digital identifier 205, during nucleic acid testing, the sample to be tested is placed in the nucleic acid reaction tube, and then the nucleic acid reaction tube is inserted into the insertion port 203 of the spinning disc 202, with the bottom of the nucleic acid reaction tube penetrating into the ring groove 104. Simultaneously, the heating ring 105 in the ring groove 104 is activated to heat the bottom of the nucleic acid reaction tube in the ring groove 104. While heating, the motor 201 is activated to drive the spinning disc 202 to rotate, thereby causing the inserted nucleic acid reaction tube to rotate and spin. This allows the nucleic acid reaction tube to be heated and rotated simultaneously above the heating ring 105, thus spinning and spinning the sample to be tested. The bottom of this tube ensures the consistency of the position of the sample to be tested in the nucleic acid reaction tube. By rotating and swinging in combination with heating, the sample can be heated more evenly in the nucleic acid reaction tube. Compared with simple static heating, it can speed up the process of the sample reaching the appropriate reaction temperature and improve the efficiency of sample pretreatment in the nucleic acid detection process. Then, the staff can observe the changes in fluorescence in the nucleic acid reaction tube through the tube lens 102. After the current nucleic acid reaction tube has been observed, the staff can record the nucleic acid reaction tube number after testing according to the number 205 on the swing plate 202. Then, the swing plate 202 can be rotated to make another set of nucleic acid reaction tubes flush with the LED beads 103 in the ring groove 104, so that the staff can observe each set of nucleic acid reaction tubes individually through the tube lens 102.

[0033] Based on the above technical solution, the working steps of this solution are summarized as follows: For nucleic acid testing, the sample to be tested is placed in a nucleic acid reaction tube, which is then inserted into the insertion port 203 of the spinning tray 202, ensuring the bottom of the nucleic acid reaction tube penetrates into the ring groove 104. Simultaneously, the heating ring 105 within the ring groove 104 heats the bottom of the nucleic acid reaction tube. While heating, the motor 201 is activated to rotate the spinning tray 202, causing the inserted nucleic acid reaction tube to rotate and spin. This rotation and spinning of the nucleic acid reaction tube, with the tube being heated above the heating ring 105, throws the sample to be tested to the bottom of the sample tube, ensuring consistent positioning of the sample within the nucleic acid reaction tube. This ensures that the analytes in the nucleic acid reaction tubes are heated to the same degree, promoting the reaction of nucleic acids with other reagents. Once the set heating time is reached, the LED beads 103 are activated to irradiate the nucleic acid reaction tubes in the ring groove 104. This allows staff to observe the changes in fluorescence in the nucleic acid reaction tubes through the tube lens 102 to determine whether a specific reaction has occurred. Based on the fluorescence reaction, staff can determine whether the target nucleic acid sequence is present in the sample, thus obtaining the test result. Staff can then record the number of the tested nucleic acid reaction tube according to the number 205 on the spinning plate 202. Then, the spinning plate 202 can be rotated to bring another set of nucleic acid reaction tubes level with the LED beads 103 in the ring groove 104, allowing staff to observe each set of nucleic acid reaction tubes individually through the tube lens 102.

[0034] In summary, the combination of rotation and shaking heating ensures the uniformity of the sample's position within the nucleic acid reaction tube, allowing for more even heating. Compared to static heating alone, this method accelerates the process of reaching the appropriate reaction temperature and improves the efficiency of sample pretreatment during nucleic acid testing.

[0035] All parts not described in this utility model are the same as or can be implemented using existing technology. Although embodiments of this utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of this utility model, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A portable nucleic acid testing device, characterized in that, include: The mounting cylinder (1) is fixedly installed with a swinging mechanism (2). A nucleic acid reaction tube can be inserted into the swinging mechanism (2). A sealing cap (101) can be inserted into the upper surface of the swinging mechanism (2) so that the sealing cap (101) can seal the nucleic acid reaction tube inside the swinging mechanism (2). The upper surface of the mounting cylinder (1) is provided with an annular groove (104) so ​​that the bottom of the nucleic acid reaction tube inserted into the swing mechanism (2) can penetrate into the annular groove (104). The upper surface of the annular groove (104) is provided with a heating ring (105) so that the heating ring (105) can be flush with the bottom of the nucleic acid reaction tube for heating. The swinging mechanism (2) includes a motor (201), which is fixedly installed in the mounting cylinder (1). A swinging disc (202) is fixedly installed on the upper surface of the output shaft of the motor (201), so that the swinging disc (202) rotates and covers the upper surface of the annular groove (104). An insertion port (203) is opened on the upper surface of the swinging disc (202), which is connected to the annular groove (104). A nucleic acid reaction tube can be inserted into the insertion port (203), so that the bottom of the nucleic acid reaction tube can penetrate into the annular groove (104). The outer surface of the swinging disc (202) is provided with a number mark (205) and an anti-slip strip (204). Each set of the number mark (205) is flush with each set of insertion ports (203).

2. The portable nucleic acid testing device according to claim 1, characterized in that: A tube lens (102) is inserted into the outer surface of the mounting tube (1), so that the other end of the tube lens (102) can be inserted into the annular groove (104) so ​​that the tube lens (102) is opposite to the bottom of the nucleic acid reaction tube. An LED bead (103) is fixedly installed at one end of the annular groove (104). The LED bead (103) is opposite to the nucleic acid reaction tube that penetrates into the annular groove (104) and irradiates it, so that the nucleic acid reaction tube that penetrates into the annular groove (104) is located between the LED bead (103) and the tube lens (102).

3. A portable nucleic acid testing device according to claim 2, characterized in that: The outer surface of the LED bead (103) is fitted with a heat insulation window (106).

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