Detachable perforated plate

Abstract

The utility model discloses a detachable perforated plate, which relates to the technical field of pretreatment devices for pesticide and veterinary drug residue and mycotoxin analysis, and comprises a perforated plate body, the perforated plate body comprises a base, SPE (Solid Phase Extraction) purification pipes and a filtering mechanism, a plurality of mounting holes are uniformly formed in the base, the SPE purification pipes are respectively and detachably arranged in the mounting holes, and the filtering mechanism is arranged in the SPE purification pipes. A filtering mechanism is arranged in each SPE purification pipe, a penetrating opening is formed in the lower side of the mounting hole, a liquid outflow end is arranged on the lower side of each SPE purification pipe, and the liquid outflow ends are inserted into the penetrating openings. Through the arrangement of the porous plate, the filtering step can be omitted, positive pressure is adopted, the liquid draining mode is achieved, and the method is used for SPE pretreatment with an impurity removal mode as a main mode; a plurality of SPE purification pipes are arranged on the porous plate, so that a plurality of samples can be treated at the same time according to actual requirements, the whole porous plate is prevented from being replaced, the experiment cost is reduced, and the efficiency is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to the pretreatment device technical field of agricultural and veterinary drug residue, mycotoxin analysis, and particularly relates to a detachable multi-well plate. BACKGROUND

[0002] The QuEChERS method is a technique for sample extraction and purification, and the basic principle is that after the homogenized sample is extracted by acetonitrile (or acidified acetonitrile), the salt is separated by salting out, the matrix dispersion extraction mechanism is used, and most of the interference substances (organic acids, fatty acids, carbohydrates, etc.) in the matrix are combined with ethylenediamine-N-propylsilane (PSA) or other adsorbents such as octadecylsilane (C18), etc., and then impurities are removed by vortex, centrifugation, and filtration, and finally GC-MS or LC-MS detection is performed. Compared with traditional sample pretreatment techniques, the QuEChERS method has the characteristics of rapidity, simplicity, low cost, high efficiency, durability, and safety, and with the deepening of scientific research, it has been widely used in the fields of pesticide residue, veterinary drug and mycotoxin and illegal additive determination.

[0003] In the traditional QuEChERS operation steps, the supernatant obtained by extraction needs to be subjected to vortex, centrifugation and filtration operations in different experimental containers in turn, and the sample liquid needs to be transferred multiple times, which not only consumes manpower, but also increases the use amount of consumables and the possibility of errors caused by multiple liquid transfers. And the current market environment has tended to be automated, efficient, fast and other operation modes, just like the prior art CN219475121U discloses a sample purification device for QuEChERS method, which comprises a syringe, a discharge pipe arranged at the bottom end of the syringe, a pushing assembly arranged at the top end of the syringe, a sealing base, a sealing top cover, and a filtering assembly; the sealing base comprises a shell, a plug body fixedly arranged in the shell, and a plug hole arranged at the top of the plug body for plugging the discharge pipe; the bottom of the shell is arc-shaped, the top is open, and the top of the shell is detachably connected with the bottom end of the syringe through threads; and the sealing top cover is detachably covered on the top end of the syringe to seal the top opening of the discharge pipe.

[0004] However, the prior art is not suitable for high-throughput and large sample size conditions, and it is time-consuming and laborious to use. UTILITY MODEL CONTENTS

[0005] The utility model aims at providing a detachable multi-well plate for solving the above technical problems.

[0006] The technical scheme adopted by the utility model is as follows:

[0007] The detachable multi-hole plate comprises a hole plate body, wherein the hole plate body comprises a base, SPE purification tubes and filtering mechanisms; the base is uniformly provided with a plurality of mounting holes; each of the mounting holes is detachably provided with the SPE purification tube; each of the SPE purification tubes is provided with the filtering mechanism; the lower side of the mounting hole is provided with a through hole; the lower side of the SPE purification tube is provided with a liquid outflow end; and the liquid outflow end is inserted into the through hole.

[0008] Preferably, the inner diameter of the upper end of the mounting hole is larger than that of the lower end.

[0009] Preferably, the inner diameter of the through hole is smaller than that of the lower end of the mounting hole.

[0010] Preferably, the upper half of the SPE purification tube is a square column tube, and the lower half of the SPE purification tube is a circular column tube.

[0011] Preferably, the SPE purification tube is a circular column tube.

[0012] Preferably, the SPE purification tube is a square column tube.

[0013] Preferably, the upper half of the SPE purification tube is an extract liquid adding area, the lower half of the SPE purification tube is a filler placing area, and the filtering mechanism is arranged in the filler placing area.

[0014] Preferably, the filtering mechanism comprises a lower sieve plate, a filler and an upper sieve plate; the lower sieve plate is arranged in the SPE purification tube and connected with the inner wall of the SPE purification tube; the upper side of the lower sieve plate is provided with the upper sieve plate; and the filler is arranged between the lower sieve plate and the upper sieve plate.

[0015] Preferably, the filtering mechanism further comprises a microporous membrane arranged on the lower side of the lower sieve plate.

[0016] The above technical scheme has the following advantages or beneficial effects:

[0017] (1) In the utility model, under the assistance of the external positive pressure device, the supernatant of the QuEChERS method can be purified and filtered, the vortex, centrifugal and filtering operations of the matrix dispersion solid phase extraction in the traditional QuEChERS method are omitted, and the liquid transfer is not needed frequently, thereby the use amount of consumables can be reduced, the sample processing efficiency is improved, and the accuracy of experimental results is improved.

[0018] (2) In the utility model, through the arrangement of the multi-hole plate, the filtering step can be omitted, the positive pressure is adopted, the liquid is drained in the mode, and the SPE pretreatment in the mode of removing impurities is used.

[0019] (3) In this utility model, several SPE purification tubes are set on the multi-well plate, which can process multiple samples at the same time according to actual needs, avoiding the replacement of the entire well plate, reducing experimental costs, and improving work efficiency.

[0020] (4) In this utility model, SPE purification tubes of different specifications can be configured to meet different throughput detection requirements;

[0021] (5) In this utility model, the base and the SPE purification tube can be disassembled from each other. It is small in size, easy to carry, and can process multiple samples at the same time. It has strong versatility and low price, which can reduce costs and increase efficiency in the laboratory. It is the future trend of QuEChERS (rapid sample pretreatment technology for agricultural product testing) and SPE (solid phase extraction) treatment. Attached Figure Description

[0022] Figure 1 This is a side view of the base in this utility model;

[0023] Figure 2 This is a bottom view of the base in this utility model;

[0024] Figure 3 yes Figure 2 Sectional view along the middle AA direction;

[0025] Figure 4 yes Figure 3 Enlarged view of point B in the middle;

[0026] Figure 5 This is a schematic diagram of the SPE purification tube in this utility model;

[0027] Figure 6 yes Figure 5 A cross-sectional view along the CC direction.

[0028] In the diagram: 1. Base; 101. Mounting hole; 102. Through outlet; 2. SPE purification pipe; 201. Liquid outflow end; 3. Filtration mechanism; 301. Lower sieve plate; 302. Packing material; 303. Upper sieve plate; 304. Microporous membrane. Detailed Implementation

[0029] The technical solution of this utility model will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0030] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0031] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0032] Figure 1 This is a side view of the base in this utility model; Figure 2 This is a bottom view of the base in this utility model; Figure 3 yes Figure 2 Sectional view along the middle AA direction; Figure 4 yes Figure 3 Enlarged view of point B in the middle; Figure 5 This is a schematic diagram of the SPE purification tube in this utility model; Figure 6 yes Figure 5 A cross-sectional view along the CC direction. See also... Figures 1 to 6The diagram illustrates a preferred embodiment of a detachable perforated plate, comprising a plate body, a base 1, an SPE purification tube 2, and a filter mechanism 3. The base 1 has a plurality of evenly distributed mounting holes 101, each mounting hole 101 containing a detachably mounted SPE purification tube 2, and each SPE purification tube 2 containing a filter mechanism 3. An outlet 102 is provided on the lower side of each mounting hole 101, and a liquid outlet 201 is provided on the lower side of each SPE purification tube 2, which is inserted into the outlet 102. In this embodiment, the base 1 is used to mount the SPE purification tube 2. The base 1 has a rectangular parallelepiped structure with a width of 85.70±0.5mm, a length of 127.80±0.5mm, and a height of 20.60±0.5mm. The SPE purification tube 2 is inserted into the mounting hole 101 and can be disassembled and replaced. The number of mounting holes 101 can be set as needed, but there must be at least two mounting holes 101. The overall shape of the mounting hole 101 is cylindrical, but the shape of the mounting hole 101 is not limited to cylindrical. The overall height of the mounting hole 101 is 19.60±0.5mm, its upper inner diameter is 14.67±0.25mm, and its lower inner diameter is 14.63±0.25mm. The inner diameter of the upper end of the mounting hole 101 is larger than that of the lower end, which facilitates the disassembly and installation of the SPE purification tube 2.

[0033] In this embodiment, the outlet 102 on the lower side of the mounting hole 101 is connected to the mounting hole 101. The inner diameter of the outlet 102 can be 4.571±0.25mm. The inner diameter of the outlet 102 is smaller than the inner diameter of the lower end of the mounting hole 101, and is used to fix the liquid outlet 201 of the SPE purification tube 2.

[0034] Furthermore, as a preferred embodiment, the upper half of the SPE purification tube 2 is a square cylindrical tube, and the lower half of the SPE purification tube 2 is a circular cylindrical tube. In this embodiment, an inner hole is formed in the middle of the SPE purification tube 2, and the inner hole is cylindrical in shape. The total length of the SPE purification tube 2 is 88.00±0.5mm, wherein the upper half and the lower half of the SPE purification tube 2 are integrally formed.

[0035] In other embodiments, the SPE purification tube 2 can be either a cylindrical tube or a square tube, depending on the specific requirements.

[0036] Furthermore, as a preferred embodiment, the upper half of the SPE purification tube 2 is the extract addition area, and the lower half of the SPE purification tube 2 is the packing material placement area, with the filtration mechanism 3 located in the packing material placement area. In this embodiment, the filtration mechanism 3 is provided for filtering and purifying the extraction supernatant from the QuEChERS method.

[0037] In use, several SPE purification tubes 2 are installed in the corresponding mounting holes 101. Then, the extraction supernatant is injected into the extraction liquid addition area, and an external positive pressure device is used for assistance. The positive pressure device applies positive pressure to the inside of the SPE purification tube 2, so that the extraction supernatant can pass through the filter mechanism 3 and flow out from the liquid outlet 201. The purified extraction supernatant can be collected by an external porous collection plate. Finally, the liquid is transferred to a sample vial for LC-MS or GC-MS analysis.

[0038] Furthermore, as a preferred embodiment, the filtration mechanism 3 includes a lower sieve plate 301, packing material 302, and an upper sieve plate 303. The lower sieve plate 301 is disposed inside the SPE purification tube 2 and connected to the inner wall of the SPE purification tube 2. The upper sieve plate 303 is disposed above the lower sieve plate 301, and the packing material 302 is disposed between the lower sieve plate 301 and the upper sieve plate 303. It also includes a microporous membrane 304, which is disposed below the lower sieve plate 301. The extraction supernatant can be filtered through the upper sieve plate 303 to remove large particulate impurities, then purified through the packing material 302 to remove impurities that interfere with detection, and then filtered again through the lower sieve plate 301 and the microporous membrane 304. After filtration, a purified extraction supernatant is formed. In this embodiment, the filtration mechanism 3 is integrated into the SPE purification tube 2, eliminating the need for frequent liquid transfer, thereby reducing the consumption of consumables and improving sample processing efficiency and the accuracy of experimental results.

[0039] In this embodiment, during use, the height of several SPE purification tubes 2 needs to be kept consistent. The inner diameter of the lower half of the SPE purification tube 2 is not limited to the 6mL SPE column tube specification, but can also be the 3mL SPE column tube specification or the 1mL SPE column tube specification. Different specifications of column tubes can be set according to the amount of packing 302.

[0040] The specific shape of the mounting hole 101 in this embodiment is not limited to a cylindrical shape, but can also be a square column shape. The specific shape can be selected as needed.

[0041] In this embodiment, both the base 1 and the SPE purification tube 2 are made of transparent PP material, which is acid and alkali resistant and not easily broken.

[0042] The above description is only a preferred embodiment of the present utility model and does not limit the implementation method and protection scope of the present utility model. Those skilled in the art should realize that all solutions obtained by equivalent substitutions and obvious changes made based on the description and illustrations of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A detachable perforated plate, characterized in that, The device includes an orifice plate body, which comprises a base, an SPE purification tube, and a filter mechanism. The base is evenly provided with a plurality of mounting holes, and the SPE purification tube is detachably installed in each mounting hole. The filter mechanism is installed in each SPE purification tube. An outlet is provided on the lower side of the mounting hole, and a liquid outflow end is provided on the lower side of the SPE purification tube. The liquid outflow end is inserted into the outlet.

2. The detachable perforated plate as described in claim 1, characterized in that, The inner diameter of the upper end of the mounting hole is larger than the inner diameter of the lower end.

3. The detachable perforated plate as described in claim 1, characterized in that, The inner diameter of the through-hole is smaller than the inner diameter of the lower end of the mounting hole.

4. The detachable perforated plate as described in claim 1, characterized in that, The upper part of the SPE purification tube is a square column tube, and the lower part of the SPE purification tube is a circular column tube.

5. The detachable perforated plate as described in claim 1, characterized in that, The SPE purification tube is a cylindrical tube.

6. The detachable perforated plate as described in claim 1, characterized in that, The SPE purification tube is a square column tube.

7. The detachable perforated plate as described in claim 1, characterized in that, The upper half of the SPE purification tube is the extract addition area, the lower half of the SPE purification tube is the packing material placement area, and the filtration mechanism is located in the packing material placement area.

8. The detachable perforated plate as described in claim 7, characterized in that, The filtration mechanism includes a lower sieve plate, packing material, and an upper sieve plate. The lower sieve plate is disposed inside the SPE purification pipe and connected to the inner wall of the SPE purification pipe. The upper sieve plate is disposed on the upper side of the lower sieve plate, and packing material is disposed between the lower sieve plate and the upper sieve plate.

9. The detachable perforated plate as described in claim 8, characterized in that, It also includes a microporous membrane, which is disposed on the lower side of the lower sieve plate.

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