Culture device for in-vitro incubation of fertilized eggs of crustacean

Abstract

The utility model discloses a culture device for in-vitro incubation of fertilized eggs of crustacean. The box cover is hinged to the box body, the heating plate, the heat preservation plate and the shelf are arranged in the box body from bottom to top, the temperature sensor is fixedly installed on the corresponding side wall of the shelf, and the culture assemblies are evenly placed in the shelf. Each culture assembly comprises a bottom support containing a culture solution, a middle plate placed on the bottom support, an upper cover arranged on the middle plate in a covering mode, a plurality of crustacean fertilized egg hatching grooves evenly formed in the middle plate in a penetrating mode and a screen fixedly laid at the bottoms of the crustacean fertilized egg hatching grooves. The cell culture device is simple in structure, can provide a stable culture environment facilitating observation of development data of a single embryo, has the same culture condition between fertilized eggs, simplifies replacement operation of culture solution, has small damage to the fertilized eggs, and is beneficial to proceeding of related experiments.

Description

Technical Field

[0001] This utility model relates to the field of crustacean seedling technology, and in particular to a culture device for in vitro incubation of crustacean fertilized eggs. Background Technology

[0002] Crustaceans, such as shrimp and crabs, have high economic value. However, in artificial breeding, the production model for most economically important crustaceans still relies primarily on incubation by the mother carrying the eggs. Hatching success depends heavily on the mother's health, leading to problems such as high abortion rates, low hatching rates, long incubation times, and inconsistent hatching times. Research indicates that fertilized crustacean eggs can develop into larvae after leaving the mother's body. Using suitable in vitro incubation devices and environmental control methods can achieve in vitro incubation of fertilized eggs.

[0003] Currently, there are four main types of in vitro incubation devices for crustacean fertilized eggs: aerated suspension devices, reverse airlift devices, circulating water devices, and circulating water + mechanical pulling devices. The first two types of devices have the problem of difficult water quality control, while the latter two types of devices have the problems of complex structure, cumbersome operation, and high cost, making it impossible to achieve precise control and observation of individual embryo development data in experimental research applications. Utility Model Content

[0004] In view of the shortcomings of the prior art described above, the purpose of this utility model is to provide a culture device for in vitro incubation of crustacean fertilized eggs that can provide a stable environment and facilitate the observation of individual embryonic development data.

[0005] A culture device for in vitro incubation of crustacean fertilized eggs, comprising a box body and a box cover hinged to the box body; the culture device further comprises:

[0006] The heating plate, insulation plate, and shelves installed inside the box from bottom to top; and

[0007] Several culture components are evenly placed in the shelf;

[0008] Each culture component includes a base containing culture medium, a middle plate placed on the base, a top cover on the middle plate, several crustacean fertilized egg hatching troughs evenly distributed on the middle plate, and a screen fixed at the bottom of the crustacean fertilized egg hatching troughs.

[0009] As a further improvement to the above solution, the shelf has several culture component placement areas, and the culture component placement areas and culture components correspond one-to-one.

[0010] As a further improvement to the above scheme, each culture component placement area is equipped with anti-slip feet at the four corners to support the base, and the anti-slip feet are fixed to the shelf.

[0011] As a further improvement to the above solution, the middle plate is affixed with labels that correspond one-to-one with the hatching tanks for crustacean fertilized eggs.

[0012] As a further improvement to the above scheme, several crustacean fertilized egg incubation tanks are distributed in a matrix.

[0013] As a further improvement to the above solution, the box is made of polyurethane, and the insulation board is made of a water-insulating bag filled with warm water.

[0014] As a further improvement to the above scheme, the culture device also includes a supplemental light and a temperature sensor fixedly installed on the corresponding side wall of the shelf.

[0015] As a further improvement to the above solution, the supplementary light is fixedly installed at the position where the lid faces the box body; a control panel is provided on the front surface of the box body, and the input terminals of the temperature sensor and the heating plate are electrically connected to the output terminal of the control panel.

[0016] Compared with the prior art, the advantages of this utility model are: simple structure, convenient operation, can provide a stable and convenient culture environment for observing the developmental data of individual embryos, and the culture conditions are the same for each fertilized egg, which also simplifies the replacement of culture medium, causes less damage to fertilized eggs, and is conducive to the conduct of related experiments. Attached Figure Description

[0017] Figure 1 The diagram shown is a schematic diagram of the in vitro incubation culture device for crustacean fertilized eggs provided by this utility model in the closed state.

[0018] Figure 2 The diagram shown is a schematic diagram of the in vitro incubation culture device for crustacean fertilized eggs provided by this utility model in the open state.

[0019] Figure 3 The image shown is an exploded view of a culture device for in vitro incubation of crustacean fertilized eggs provided by this utility model.

[0020] Figure 4 As shown Figure 3 Structural diagram of the middle shelf.

[0021] Figure 5 As shown Figure 3 A structural diagram of the culture component.

[0022] Figure 6 As shown Figure 5 Exploded view.

[0023] Explanation of main component symbols

[0024] 1. Box body; 2. Box lid; 3. Supplemental light; 4. Heating plate; 5. Insulation plate; 6. Shelf; 7. Temperature sensor; 8. Anti-slip feet; 9. Culture components; 91. Base tray; 92. Middle plate; 93. Top cover; 94. Crustacean fertilized egg incubator; 95. Screen; 10. Label.

[0025] The above description of the main component symbols, together with the accompanying drawings and specific embodiments, provides a further detailed explanation of this utility model. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of this utility model clearer, the following detailed description is provided in conjunction with embodiments. Additional aspects and advantages of this utility model will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the utility model. It should be understood that the following description is merely illustrative and not intended to limit the utility model.

[0027] The specific embodiments of this utility model are described in detail below.

[0028] Please see Figures 1-4 This embodiment provides a culture device for in vitro incubation of crustacean fertilized eggs, which includes a box body 1, a box cover 2 hinged to the box body 1, a supplementary light 3, a heating plate 4, an insulation plate 5 and a shelf 6 installed from bottom to top in the box body 1, a temperature sensor 7 fixedly installed on the corresponding side wall of the shelf 6, and a plurality of culture components 9 evenly placed in the shelf 6.

[0029] This embodiment uses a box 1 with a wall thickness of 2.0cm, external dimensions of 40*30*28cm, and internal dimensions of 36*26*24cm as an example. The box 1 is made of polyurethane, which has good heat insulation properties. The supplementary light 3 is fixedly installed on the box cover 2 facing the box 1. In this embodiment, the supplementary light 3 uses an adjustable LED light panel as an example. The supplementary light 3 provides a suitable incubation environment for fertilized eggs that require a certain light intensity to hatch.

[0030] This embodiment uses a heating plate 4 with dimensions of 36*26*1cm as an example. The heating plate 4 is made of waterproof aluminum foil, which meets the waterproof requirements. The heating plate 4 plays a role in regulating the temperature of the cultivation environment inside the chamber 1. In this embodiment, the insulation plate 5 is an insulated water bag filled with warm water. The insulation plate 5 plays a role in heat transfer, and it can completely cover the shelf 6, so that the cultivation components 9 inside the shelf 6 are heated evenly.

[0031] The shelf 6 has a number of culture component placement areas, and the culture component placement areas correspond to the culture components 9 one by one. Anti-slip feet 8 for supporting the bottom tray 91 are provided at the four corners of each culture component placement area, and the anti-slip feet 8 are fixed to the shelf 6. In this embodiment, the shelf 6 enables the stable placement of the culture components 9 in the box body 1. While facilitating the taking and placing of the culture components 9, it can prevent the culture components 9 from directly contacting the insulation board 5, preventing uneven culture temperature and contamination of the insulation board 5.

[0032] In this embodiment, a control panel is provided on the front surface of the box body 1. The input ends of the temperature sensor 7 and the heating plate 4 are electrically connected to the output end of the control panel. The working temperature of the heating plate 4 is set through the control panel. Moreover, the ambient temperature detected by the temperature sensor 7 is shown on the display screen of the control panel for timely adjustment. The electrical control method among the control panel, the heating plate 4, and the temperature sensor 7 is a mature existing technology and will not be elaborated here. In this embodiment, the temperature sensor 7 with the model number NST1001 is taken as an example for illustration. Based on the temperature sensor 7, the temperature of the environment where the culture component 9 is located is detected, so as to facilitate accurate temperature control.

[0033] Please continue to refer to Figures 5-6 , each culture component 9 includes a bottom tray 91 filled with culture solution, a middle plate 92 placed on the bottom tray 91, an upper cover 93 covering the middle plate 92, a number of crustacean fertilized egg incubation grooves 94 uniformly and penetratingly opened on the middle plate 92, and a screen 95 fixedly laid at the bottom of the crustacean fertilized egg incubation grooves 94. The number of crustacean fertilized egg incubation grooves 94 are distributed in a matrix. The crustacean fertilized egg incubation grooves 94 in this embodiment are circular holes, with the radius r of the circular surface being 0.3 cm < r < 1 cm and the hole depth H being 1 cm < H < 1.8 cm. A label 10 corresponding to the crustacean fertilized egg incubation grooves 94 is pasted on the middle plate 92. Based on the label 10, the information of the fertilized eggs cultured in the corresponding crustacean fertilized egg incubation grooves 94 is marked to conduct a rigorous research experiment on crustacean seedling cultivation.

[0034] The culture component 9 in this embodiment has a three-layer design consisting of a base 91, a middle plate 92, and a top cover 93 stacked from bottom to top. The top cover 93 effectively isolates external impurities and microorganisms from entering the in vitro incubation environment. The middle plate 92 has multiple independent crustacean fertilized egg incubation tanks 94, which effectively prevents large-scale clumping of fertilized eggs, thereby improving the hatching rate. The base 91 is used to hold the culture medium, ensuring that the culture environment in each crustacean fertilized egg incubation tank 94 is the same, which is beneficial for related experiments. At the same time, the design of the base 91 facilitates the replacement of the culture medium. When replacing the culture medium, only a new base 91 needs to be prepared, the culture medium added, and the original middle plate 92 transferred to the new base 91, simplifying the operation process and reducing damage to the fertilized eggs. This embodiment uses an 80-mesh sieve 95 as an example. The sieve 95 serves two purposes: firstly, it holds the fertilized eggs to prevent them from passing through; secondly, it allows the culture medium to pass through the sieve 95 and submerge the fertilized eggs, preventing them from drying out and thus ensuring the survival rate.

[0035] The following describes the use of the culture apparatus of this embodiment for in vitro incubation of crustacean fertilized eggs, and the specific usage process is as follows:

[0036] First, place the insulation board 5 filled with warm water, the shelf 6, and the culture component 9 containing culture medium into the box 1 in sequence, and close the top cover 2.

[0037] Next, turn on the power and adjust the device temperature to a suitable temperature for the fertilized eggs to be incubated, and preheat for 15 minutes;

[0038] Then, take out the culture component 9, lift the top cover 93 to expose the middle plate 92, and add the separated single or multiple fertilized eggs to the crustacean fertilized egg incubation tank 94 in sequence according to the experimental requirements. After replacing the top cover 93, put it back on the shelf 6, close the box cover 2, and adjust the appropriate light intensity to start incubation.

[0039] Finally, change the culture medium daily. The procedure for changing the culture medium is as follows: take a clean base tray 91 and top cover 93, add fresh culture medium (such as seawater), place it in the device and preheat for 15 minutes, then transfer the middle plate 92 containing the fertilized eggs to the new base tray 91.

[0040] In summary, the culture device of this embodiment has the following advantages: it has a simple structure, is easy to operate, can provide a stable and convenient culture environment for observing individual embryo development data, and the culture conditions are the same for each fertilized egg. It also simplifies the replacement of culture medium, causes less damage to the fertilized eggs, and is beneficial to the conduct of related experiments.

[0041] The preferred embodiments of this utility model disclosed above are merely illustrative of the present utility model. These preferred embodiments do not exhaustively describe all details, nor do they limit the utility model to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of this utility model, thereby enabling those skilled in the art to better understand and utilize it. This utility model is limited only by the claims and their full scope and equivalents.

Claims

1. A culture device for in vitro incubation of crustacean fertilized eggs, comprising a box body (1) and a box cover (2) hinged to the box body (1); Its features are, The culture apparatus further includes: The heating plate (4), insulation plate (5), and shelf (6) installed from bottom to top inside the housing (1); and Several culture components (9) are evenly placed in the shelf (6); Each culture component (9) includes a base (91) containing culture medium, a middle plate (92) placed on the base (91), a top cover (93) covering the middle plate (92), several crustacean fertilized egg hatching troughs (94) evenly opened on the middle plate (92), and a screen (95) laid and fixed at the bottom of the crustacean fertilized egg hatching troughs (94).

2. The in vitro incubation and culture device for crustacean fertilized eggs according to claim 1, characterized in that, The shelf (6) has several culture component placement areas, and the culture component placement areas and culture components (9) correspond one-to-one.

3. The in vitro incubation and culture device for crustacean fertilized eggs according to claim 2, characterized in that, Each culture component placement area is provided with anti-slip feet (8) at the four corners to support the base (91), and the anti-slip feet (8) and the shelf (6) are fixed together.

4. The in vitro incubation and culture device for crustacean fertilized eggs according to claim 1, characterized in that, The middle plate (92) has a label (10) that corresponds one-to-one with the crustacean fertilized egg incubation tank (94).

5. The in vitro incubation and culture device for crustacean fertilized eggs according to claim 4, characterized in that, Several crustacean fertilized egg incubators (94) are distributed in a matrix.

6. The in vitro incubation and culture device for crustacean fertilized eggs according to claim 1, characterized in that, The box body (1) is made of polyurethane, and the insulation board (5) is made of a water-insulating bag, which contains warm water.

7. The in vitro incubation and culture device for crustacean fertilized eggs according to claim 1, characterized in that, The culture device also includes a supplemental light (3) and a temperature sensor (7) fixedly installed on the corresponding side wall of the shelf (6).

8. The in vitro incubation and culture apparatus for crustacean fertilized eggs according to claim 7, characterized in that, The supplementary light (3) is fixedly installed on the lid (2) facing the box body (1); A control panel is provided on the front surface of the housing (1), and the input terminals of the temperature sensor (7) and the heating plate (4) are electrically connected to the output terminal of the control panel.

Images