Novel affinity chromatography column
By setting a side plate and support frame at the bottom of the affinity chromatography column, and using a positioning semi-ring and insert ring structure to fix the drainage tube, the problem of the drainage tube loosening and falling off was solved, a more stable drainage process was achieved, and the cleaning operation was simplified.
Patent Information
- Application Number
- CN202423240940.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-26
- Publication Date
- 2025-12-12
- Estimated Expiration
- 2034-12-26
AI Technical Summary
Existing affinity chromatography columns lack a supporting structure between the drainage tube and the chromatography column during use, leading to loosening or even detachment after prolonged drainage, resulting in poor stability.
Side plates and support frames are installed at the bottom of the chromatography column. The drainage tube is fixed by a combination of positioning half-rings and insert rings to enhance stability, and a stable connection is achieved through the cooperation of sliders and fixing rods.
It effectively prevents loosening and detachment between the drainage tube and the chromatography column, improves the stability of the drainage process, and simplifies the cleaning process through the damping rod and scraper structure.
Smart Images
Figure CN223654497U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to affinity chromatography column technical field, especially a kind of novel affinity chromatography column. BACKGROUND
[0002] Affinity chromatography column is a kind of separation and purification technique widely used in biochemistry and molecular biology field, and fish IgM monoclonal antibody is a kind of specific antibody to fish immunoglobulin M, needs to use chromatography column to extract antibody.
[0003] The existing affinity chromatography column needs to install drainage tube at the bottom of chromatography column to drain fish Igm monoclonal antibody extracted in actual use, and there is no supporting structure between drainage tube and chromatography column, which will cause loosening between drainage tube and chromatography column after long time drainage, and even cause drainage tube to fall off in serious case, so a new type of affinity chromatography column is needed to reinforce the installed drainage tube and improve the stability during drainage. UTILITY MODEL CONTENT
[0004] The utility model aims at providing a kind of novel affinity chromatography column, to solve the problem that the existing affinity chromatography column needs to install drainage tube at the bottom of chromatography column to drain fish Igm monoclonal antibody extracted in actual use in the above background art, and there is no supporting structure between drainage tube and chromatography column, which will cause loosening between drainage tube and chromatography column after long time drainage, and even cause drainage tube to fall off in serious case, poor stability.
[0005] To solve the above technical problems, the utility model is realized by the following technical solutions:
[0006] The utility model is a kind of novel affinity chromatography column, comprising:
[0007] Chromatography column body;
[0008] Positioning component, the positioning component includes side plate, support frame, support disc, positioning half ring, limit ring, sliding block, insert ring, slot and drainage tube, the drainage tube is connected at the bottom of chromatography column body, the lower part of both sides of chromatography column body outer surface is connected with side plate, the inside of side plate is equipped with slot, sliding block is movably connected in the inside of slot, the outside surface of sliding block is connected with support frame on one side, the lower part of one side of support frame is connected with positioning half ring, the top of drainage tube is connected with support disc, support disc is connected at the bottom of chromatography column body, and positioning half ring is clamped and connected at the bottom of support disc.
[0009] Further, the top of the positioning half ring is connected with the limit ring, and the limit ring is connected on both sides of the outer surface of the support disc.
[0010] Further, the bottom of the chromatography column body is symmetrically provided with a slot, and the top of the limiting ring is connected with an inserting ring which is inserted into the slot.
[0011] Further, the fixed component comprises a sliding groove, a sliding block, a fixed hole, a fixed rod and a fixed ring, the upper portion of the inner surface of the sliding groove is provided with the fixed hole, the top of the sliding block is connected with the fixed rod which is inserted into the fixed hole, and the outer surface of the fixed rod is tightly connected with the fixed ring.
[0012] Further, the both ends of the inner surface of the sliding groove are provided with the sliding groove, and the both ends of the outer surface of the sliding block are connected with the sliding block which is movably connected with the sliding groove.
[0013] Further, the cleaning component comprises a top cover, a damping ring, a damping rod, a handle, a supporting rod, a scraper, a sleeve ring, an internal thread ring, a threaded rod and a handle, the top cover is connected with the top of the chromatography column body, the middle portion of the top end of the top cover is connected with the damping ring, the inner portion of the damping ring is connected with the damping rod, the top of the damping rod is fixedly connected with the handle, the bottom of the damping rod is connected with the scraper, the middle portion of the inner surface of the scraping ring is connected with the sleeve ring, the sleeve ring is connected with the lower portion of the outer surface of the damping rod in a sleeving mode, and a plurality of supporting rods are connected between the sleeve ring and the scraper.
[0014] Further, the middle portion of one end of the outer surface of the sleeve ring is connected with the internal thread ring, the inner portion of the internal thread ring is connected with the threaded rod, and the outer end of the threaded rod is rotatably connected with the handle.
[0015] Compared with the prior art, the chromatography column body has the following advantages:
[0016] The chromatography column body has the following advantages:
[0017] Based on the above advantages, the chromatography column body is provided with the damping rod and the scraping ring, and after the chromatography column body is used for a long time, some liquid is attached to the inner wall of the chromatography column body, the user holds the handle, moves the damping rod up and down in the damping ring, and scrapes the liquid attached to the inner wall of the chromatography column body by the scraper, so that the convenience during cleaning is improved. BRIEF DESCRIPTION OF DRAWINGS
[0018] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0019] Figure 1 This is a schematic diagram of the overall chromatography column of this utility model;
[0020] Figure 2 This is a schematic diagram of the bottom of the chromatography column of this utility model;
[0021] Figure 3 This is a schematic diagram of the positioning semi-ring of this utility model;
[0022] Figure 4 This is a schematic diagram of the damping rod and scraper of this utility model after disassembly.
[0023] The attached diagram lists the components represented by each number as follows:
[0024] 11. Chromatography column;
[0025] 21. Side plate; 22. Support frame; 23. Support plate; 24. Positioning half ring; 25. Limiting ring; 26. Slider; 27. Insert ring; 28. Slot; 29. Slide groove; 291. Drainage tube;
[0026] 31. Moving groove; 32. Moving block; 33. Fixing hole; 34. Fixing rod; 35. Fixing ring;
[0027] 41. Top cover; 42. Damping ring; 43. Damping rod; 44. Handle; 45. Support rod; 46. Scraper ring; 47. Collar; 48. Internal threaded ring; 49. Threaded rod; 491. Handle. Detailed Implementation
[0028] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings.
[0029] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.
[0030] To make the objectives, technical solutions, and advantages of this utility model clearer, the embodiments of this utility model will be described in further detail below with reference to the accompanying drawings.
[0031] Please see Figures 1-3 As shown, this embodiment is a novel affinity chromatography column, comprising:
[0032] Chromatography column 11;
[0033] The positioning component includes a side plate 21, a support frame 22, a support plate 23, a positioning half ring 24, a limiting ring 25, a slider 26, a insertion ring 27, a slot 28, a groove 29, and a drainage tube 291. The drainage tube 291 is connected to the bottom of the chromatography column 11. The lower parts of both sides of the outer surface of the chromatography column 11 are connected to the side plate 21. The inside of the side plate 21 is provided with a groove 29. The slider 26 is movably connected to the inside of the groove 29. The support frame 22 is connected to one side of the outer surface of the slider 26. The positioning half ring 24 is connected to the lower part of one side of the support frame 22. The top of the drainage tube 291 is connected to the support plate 23. The support plate 23 is connected to the bottom of the chromatography column 11. The positioning half ring 24 is clamped and connected to the bottom of the support plate 23.
[0034] The drainage tube 291 is installed at the bottom of the chromatography column 11, and the support plate 23 is attached to the bottom of the chromatography column 11. Then, the slider 26 slides inside the groove 29. According to the position of the support plate 23, the positioning half ring 24 is moved and clamped on both sides of the outer surface of the support plate 23 to fix the support plate 23.
[0035] The top of the positioning semi-ring 24 is connected to a limiting ring 25, which is connected to both sides of the outer surface of the support plate 23;
[0036] After the positioning half-ring 24 is clamped on both sides of the outer surface of the support plate 23, the limiting ring 25 will simultaneously fit and fasten to both sides of the support plate 23 for positioning.
[0037] The bottom of the chromatography column 11 is symmetrically provided with slots 28, and the top of the limiting ring 25 is connected to a plug ring 27, which is inserted into the inside of the slot 28.
[0038] After the positioning half-ring 24 is clamped on the outside of the support plate 23, the insertion ring 27 is simultaneously inserted into the inside of the slot 28 for fixation.
[0039] It also includes a fixing component, which includes a moving groove 31, a moving block 32, a fixing hole 33, a fixing rod 34 and a fixing ring 35. The upper part of the inner surface of the sliding groove 29 is provided with a fixing hole 33, the top of the slider 26 is connected to a fixing rod 34, the fixing rod 34 is inserted into the inside of the fixing hole 33, and the outer surface of the fixing rod 34 is fastened to a fixing ring 35.
[0040] The fixing rod 34 is inserted into the fixing hole 33, and then the fixing ring 35 is fastened to the outside of the fixing rod 34 to fix the position of the moving positioning half ring 24.
[0041] The inner surface of the slide groove 29 has two moving grooves 31 at both ends, and the outer surface of the slider 26 has two moving blocks 32 connected to both ends. The moving blocks 32 are movably connected inside the moving grooves 31.
[0042] When the slider 26 moves inside the slide groove 29, its moving block 32 moves simultaneously inside the moving groove 31, providing support.
[0043] The working principle is as follows: First, the drainage tube 291 is installed at the bottom of the chromatography column 11, and the support plate 23 is attached to the bottom of the chromatography column 11. Then, the slider 26 slides inside the groove 29. According to the position of the support plate 23, the positioning half ring 24 is moved and clamped on both sides of the outer surface of the support plate 23, while the insertion ring 27 is inserted into the slot 28. The fixing rod 34 is fixed inside the fixing hole 33, and the fixing ring 35 is fastened to the outside of the fixing rod 34 to fix the position of the positioning half ring 24 and fix the support plate 23. After a long period of drainage, this prevents the drainage tube 291 from becoming loose between the chromatography column, which could even lead to the drainage tube 291 detaching in severe cases, thus improving the stability during drainage. Finally, the fixing ring 35 is loosened, and the slider 26 moves inside the groove 29, while the moving block 32 moves inside the moving groove 31, moving the positioning half ring 24 to separate it from the support plate 23, so that the drainage tube 291 can be removed.
[0044] Please see Figure 1 , Figure 4 As shown, this embodiment is based on the above embodiment 1, and further includes a cleaning component. The cleaning component includes a top cover 41, a damping ring 42, a damping rod 43, a handle 44, a support rod 45, a scraper, a collar 47, an internal threaded ring 48, a threaded rod 49, and a handle 491. The top cover 41 is connected to the top of the chromatography column 11. The damping ring 42 is connected to the middle of the top of the top of the top cover 41. The damping rod 43 is connected inside the damping ring 42. The handle 44 is fixedly connected to the top of the damping rod 43. The scraper is connected to the bottom of the damping rod 43. The collar 47 is connected to the middle of the inner surface of the scraper ring 46. The collar 47 is connected to the lower part of the outer surface of the damping rod 43 by a sleeve. Multiple support rods 45 are connected between the collar 47 and the scraper.
[0045] The user holds the handle 44 and moves the damping rod 43 up and down repeatedly inside the damping ring 42, using the internal scraper to scrape off the liquid adhering to the inner wall of the chromatography column 11.
[0046] An internal threaded ring 48 is connected to the middle of one end of the outer surface of the collar 47. A threaded rod 49 is connected inside the internal threaded ring 48. A handle 491 is rotatably connected to the outer end of the threaded rod 49.
[0047] The scraper ring 46 can be fixed after installation by being fastened to the outside of the damping rod 43 by the threaded rod 49.
[0048] The working principle is as follows: After prolonged use, some liquid will adhere to the inner wall of the chromatography column 11. The damping rod 43 is inserted into the damping ring 42, and then the collar 47 is fitted onto the bottom of the damping rod 43. The threaded rod 49 is rotated and clamped to the outside of the damping rod 43, which can fix the scraper ring 46 after installation. The user holds the handle 44 and moves the damping rod 43 up and down repeatedly inside the damping ring 42. The internal scraper is used to scrape off the liquid adhering to the inner wall of the chromatography column 11, improving the convenience of cleaning.
[0049] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.
[0050] Finally, it should be noted that the above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.
Claims
1. A novel affinity chromatography column, characterized in that, include: Chromatography column (11); The positioning component includes a side plate (21), a support frame (22), a support plate (23), a positioning half ring (24), a limiting ring (25), a slider (26), a insertion ring (27), a slot (28), a groove (29), and a drainage tube (291). The drainage tube (291) is connected to the bottom of the chromatography column (11). The lower part of both sides of the outer surface of the chromatography column (11) is connected to the side plate (21). The inside of the side plate (21) is provided with a groove (29). The slider (26) is movably connected to the inside of the groove (29). The support frame (22) is connected to one side of the outer surface of the slider (26). The positioning half ring (24) is connected to the lower part of one side of the support frame (22). The top of the drainage tube (291) is connected to the support plate (23). The support plate (23) is connected to the bottom of the chromatography column (11). The positioning half ring (24) is clamped and connected to the bottom of the support plate (23).
2. The novel affinity chromatography column according to claim 1, characterized in that, The top of the positioning half-ring (24) is connected to a limiting ring (25), which is connected to both sides of the outer surface of the support plate (23).
3. A novel affinity chromatography column according to claim 1, characterized in that, The bottom of the chromatography column (11) is symmetrically provided with slots (28), and the top of the limiting ring (25) is connected to a plug ring (27), which is inserted into the inside of the slot (28).
4. A novel affinity chromatography column according to claim 1, characterized in that, It also includes a fixing component, which includes a moving groove (31), a moving block (32), a fixing hole (33), a fixing rod (34), and a fixing ring (35). The upper part of the inner surface of the sliding groove (29) is provided with a fixing hole (33), the top of the slider (26) is connected to a fixing rod (34), the fixing rod (34) is inserted into the inside of the fixing hole (33), and the outer surface of the fixing rod (34) is tightly connected to a fixing ring (35).
5. A novel affinity chromatography column according to claim 4, characterized in that, The inner surface of the slide groove (29) has two movable grooves (31) at both ends, and the outer surface of the slider (26) is connected to two movable blocks (32), which are movably connected inside the movable grooves (31).
6. A novel affinity chromatography column according to claim 1, characterized in that, It also includes a cleaning component, which includes a top cover (41), a damping ring (42), a damping rod (43), a handle (44), a support rod (45), a scraper, a collar (47), an internal threaded ring (48), a threaded rod (49), and a handle (491). The top cover (41) is connected to the top of the chromatography column (11). The middle of the top of the top of the top cover (41) is connected to the damping ring (42). The inside of the damping ring (42) is connected to the damping rod (43). The top of the damping rod (43) is fixedly connected to the handle (44). The bottom of the damping rod (43) is connected to the scraper. The middle of the inner surface of the scraper ring (46) is connected to the collar (47). The collar (47) is connected to the lower part of the outer surface of the damping rod (43) by a sleeve. Multiple support rods (45) are connected between the collar (47) and the scraper.
7. A novel affinity chromatography column according to claim 6, characterized in that, An internal threaded ring (48) is connected to the middle of one end of the outer surface of the collar (47). A threaded rod (49) is connected inside the internal threaded ring (48). A handle (491) is rotatably connected to the outer end of the threaded rod (49).