Escherichia coli culture fermentation container
By setting up a combination of an air guide ring and a pressure cylinder inside the culture tank, the problem of uneven oxygen distribution during E. coli culture was solved, achieving all-round oxygen supply and improving culture efficiency and cell quality.
Patent Information
- Application Number
- CN202522321910.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-11-03
- Publication Date
- 2025-12-12
- Estimated Expiration
- 2035-11-03
AI Technical Summary
During the cultivation of E. coli, oxygen is difficult to distribute evenly into the fermentation broth, resulting in insufficient dissolved oxygen, which affects the growth and metabolism of the bacteria. Existing stirring devices cannot effectively increase oxygen supply.
By setting gas guide rings at different heights inside the culture tank, pressurizing the oxygen through a pressure cylinder, and then spraying high-pressure oxygen through the gas guide rings, the oxygen is ensured to be evenly distributed in all areas of the culture tank. The combination of the gas guide rings and the pressure cylinder enables all-round oxygen supply.
It improves the uniformity of oxygen distribution during E. coli culture, ensuring a good environment for bacterial growth and metabolism, and improving culture efficiency and bacterial quality.
Smart Images
Figure CN223660077U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to the technical field of escherichia coli culture, especially to an escherichia coli culture and fermentation container. BACKGROUND
[0002] Escherichia coli is a gram-negative, facultative anaerobic enteric bacillus. It has an extremely important position in biotechnology and industrial production, and is often used as a host bacterium for genetic engineering to produce various recombinant proteins, enzymes, biofuels and drugs. The growth and metabolism of escherichia coli require oxygen, and if the dissolved oxygen is insufficient, it will affect the metabolic activity of the bacterial cells, thereby causing the synthesis of products to be blocked. Therefore, during the culture of escherichia coli, oxygen needs to be continuously added, but the injection of oxygen into escherichia coli is mostly located in the upper layer of the inside of the fermentation device, and the oxygen cannot reach the inside of the fermentation broth. In order to increase the oxygen content inside the fermentation broth, a stirring device is also provided for stirring, which limits the internal space of the fermentation tank, and even if the stirring device is provided, escherichia coli reproduces every twenty minutes, and the oxygen consumption is large, so simple stirring cannot increase the supply of oxygen too much. SUMMARY
[0003] The utility model aims at providing a kind of escherichia coli culture and fermentation container, gas guide ring is arranged at different height in culture tank, and the oxygen supplement is pressurized by pressurizing cylinder, and is sprayed by gas guide ring, which can solve the problems in the above background technology in different positions.
[0004] The utility model discloses a kind of escherichia coli culture and fermentation container, including culture tank, at least two groups of gas guide rings are provided on the inner side wall of the culture tank, for providing oxygen inside culture tank, the outer side wall of the upper end of the culture tank is fixedly connected with pressurizing cylinder, pressurizing piston is slidably connected in the pressurizing cylinder, for the oxygen that enters the inside of pressurizing cylinder is compressed and pressurized;
[0005] Air inlet is provided on the side wall of the pressurizing cylinder, the end of the pressurizing piston away from air inlet is fixedly connected with adjusting cylinder, the top end of the pressurizing cylinder away from culture tank is equipped with electric push rod, the power output end of the electric push rod is threadedly connected with adjusting cylinder, for changing the position of adjusting cylinder on the power output end of electric push rod, and changing the stroke distance of pressurizing piston;
[0006] Gas guide cavity is arranged in the gas guide ring, rubber exhaust head is inserted on the inner side wall of the gas guide ring, communication hole is arranged in the middle of the rubber exhaust head, for the high-pressure oxygen in gas guide cavity is discharged into culture tank, second connecting pipe is connected in the middle of the gas guide ring.
[0007] Preferably, the adjusting cylinder is located outside the pressurizing cylinder towards the top end of the electric push rod, the pressurizing piston is located at the upper end of the air inlet hole, the pressurizing piston is located between the air inlet hole and the adjusting cylinder when the electric push rod is not working, and the end of the adjusting cylinder towards the electric push rod is fixedly connected with a rotating ring.
[0008] Preferably, the two ends of the second connecting pipe are threaded structures, a first connecting pipe is arranged between the two adjacent air guide rings for controlling the distance between the two air guide rings, and the two ends of the first connecting pipe are connected with the second connecting pipes on the two air guide rings respectively for guiding the oxygen in the pressurizing cylinder into each air guide ring.
[0009] Preferably, the pressurizing cylinder is fixedly connected with an air pipe towards the top end of the culture tank, the top end of the air pipe is threadedly connected with the closest second connecting pipe, and the lower end of the second connecting pipe at the lowest position is threadedly connected with a sealing cover for controlling the sealing performance of the oxygen guided into the first connecting pipe and the second connecting pipe.
[0010] Preferably, the air guide cavity is arranged around the air guide ring, and the second connecting pipe is provided with an air outlet hole at the position of the air guide cavity.
[0011] Preferably, the end of the communication hole towards the air guide cavity is a horn-shaped structure, facilitating the pressurized oxygen to enter the rubber exhaust head, and the end of the rubber exhaust head away from the air guide ring is an inclined surface for bearing external pressure and closing the communication hole at the position of the inclined surface.
[0012] Preferably, the outer wall of the rubber exhaust head is fixedly connected with a fixing ring, and the fixing ring is fixedly connected with the inner wall of the air guide ring.
[0013] Preferably, the end of the pressurizing cylinder away from the culture tank is fixedly connected with a support frame, the support frame is a U-shaped structure, the electric push rod is fixedly connected to the middle position of the support frame, and the outer wall of the pressurizing cylinder is fixedly connected with a one-way valve at the position of the air inlet hole.
[0014] The culture tank and the pressurizing cylinder are connected as shown in the schematic view. The culture tank and the pressurizing cylinder are connected as shown in the schematic view.
[0015] Figure 1 The culture tank and the pressurizing cylinder are connected as shown in the schematic view.
[0016] The culture tank and the pressurizing cylinder are connected as shown in the schematic view.Figure 2 is the schematic diagram of oxygen pressurization connection in the pressurizing cylinder.
[0017] Figure 3 is the schematic diagram of internal connection of the culture tank.
[0018] Figure 4 is the schematic diagram of connection of the air guide ring and the rubber exhaust head.
[0019] Figure 5 is the sectional view of the rubber exhaust head.
[0020] In the figure: 1 culture tank, 2 air guide ring, 3 pressurizing cylinder, 4 air pipe, 5 support frame, 6 electric push rod, 7 pressurizing piston, 8 adjusting cylinder, 9 air inlet hole, 10 one-way valve, 11 first connecting pipe, 12 air guide cavity, 13 second connecting pipe, 14 exhaust hole, 15 rubber exhaust head, 16 communication hole, 17 fixing ring. DETAILED DESCRIPTION
[0021] In order to make the purpose, technical scheme and advantages of the utility model more clear and obvious, the utility model is further described in detail below in combination with the drawings and examples. It should be understood that the specific examples described herein are only used to explain the utility model, and are not used to limit the utility model.
[0022] Referring to Figures 1-5 A kind of E. coli culture fermentation vessel provided by the utility model, including culture tank 1, it is characterized in that: at least two groups of air guide rings 2 are equipped on the inner side wall of culture tank 1, for providing oxygen in culture tank 1 inside, air guide ring 2 is arranged at different heights of culture tank 1, when air guide ring 2 sprays oxygen, oxygen can be fully mixed with fermentation broth, it is helpful for E. coli to obtain oxygen more evenly in culture process, the upper end outer side wall of culture tank 1 is fixedly connected with pressurizing cylinder 3, the method of fixed connection can also be welding, riveting, screwing or gluing etc.;
[0023] Pressurizing piston 7 is slidably connected in pressurizing cylinder 3, for compressing and pressurizing oxygen entering the inside of pressurizing cylinder 3, after pressurized oxygen reaches air guide ring 2, it can be sprayed out of air guide ring 2, so that oxygen is fully mixed with fermentation broth;
[0024] The side wall of the pressurizing cylinder 3 is provided with an air inlet hole 9, which is connected with an oxygen storage box through a pipeline and an air pump. Oxygen is input into the pressurizing cylinder 3 through the air pump and the air pump. The end of the pressurizing piston 7 away from the air inlet hole 9 is fixedly connected with an adjusting cylinder 8. The top end of the pressurizing cylinder 3 away from the culture tank 1 is provided with an electric push rod 6. The end of the pressurizing cylinder 3 away from the culture tank 1 is fixedly connected with a support frame 5, which is in a U-shaped structure. The electric push rod 6 is fixedly connected to the middle part of the support frame 5. The outer side wall of the pressurizing cylinder 3 at the position of the air inlet hole 9 is fixedly connected with a one-way valve 10. The support frame 5 can fix the electric push rod 6 and facilitate the rotation adjustment of the adjusting cylinder 8.
[0025] The power output end of the electric push rod 6 is threadedly connected with the adjusting cylinder 8, so as to change the position of the adjusting cylinder 8 on the power output end of the electric push rod 6 and change the stroke distance of the pressurizing piston 7. The top end of the adjusting cylinder 8 towards the electric push rod 6 is located outside the pressurizing cylinder 3. The pressurizing piston 7 is located at the upper end of the air inlet hole 9. When the electric push rod 6 is not working, the pressurizing piston 7 is located between the air inlet hole 9 and the adjusting cylinder 8. The end of the adjusting cylinder 8 towards the electric push rod 6 is fixedly connected with a rotating ring. When the electric push rod works, the pressurizing piston 7 can be driven to reciprocate in the pressurizing cylinder 3 through the adjusting cylinder 8, so as to compress and pressurize the oxygen entering the pressurizing cylinder 3. The one-way valve 10 can limit the flow direction of the oxygen, so as to avoid the oxygen from being discharged from the air inlet hole 9 when the oxygen in the pressurizing cylinder 3 is pressurized. When it is needed to change the oxygen pressure in the pressurizing cylinder 3 and the gas guide ring 2, the rotating ring is manually twisted by workers, so as to drive the adjusting cylinder 8 to rotate, thereby changing the position height of the pressurizing piston 7 in the pressurizing cylinder 3 and changing the stroke distance of the pressurizing piston, so as to increase the oxygen in the pressurizing cylinder 3 and the gas guide ring 2 to different pressures.
[0026] The middle part of the gas guide ring 2 is connected with a second connecting pipe 13. The two ends of the second connecting pipe 13 are in a threaded structure. The first connecting pipe 11 is arranged between the two adjacent gas guide rings 2, so as to control the distance between the two gas guide rings 2. The two ends of the first connecting pipe 11 are respectively connected with the second connecting pipes 13 on the two gas guide rings 2, so as to guide the oxygen in the pressurizing cylinder 3 into each gas guide ring 2. The top end of the pressurizing cylinder 3 towards the culture tank 1 is fixedly connected with an air pipe 4. The top end of the air pipe 4 is threadedly connected with the closest second connecting pipe 13. The lower end of the second connecting pipe 13 at the lowest position is threadedly connected with a sealing cover, so as to control the sealing performance of the oxygen introduced into the first connecting pipe 11 and the second connecting pipe 13. The gas guide cavity 12 is arranged around the gas guide ring 2. The second connecting pipe 13 at the position of the gas guide cavity 12 is provided with an exhaust hole 14. The first connecting pipe 11 can connect the two adjacent gas guide rings 2. The air pipe 4 can guide the oxygen from the pressurizing cylinder 3 into the gas guide ring 2, so as to supply the oxygen to the multiple areas and all directions in the culture tank 1, so as to adapt to the culture of E. coli of different scales and types and improve the universality and applicability of the equipment.
[0027] The gas guide ring 2 has a gas guide chamber 12 inside. A rubber exhaust head 15 is inserted into the inner wall of the gas guide ring 2. A through hole 16 is provided in the middle of the rubber exhaust head 15 to discharge high-pressure oxygen from the gas guide chamber 12 into the culture tank 1. The end of the through hole 16 facing the gas guide chamber 12 has a funnel-shaped structure to facilitate the entry of pressurized oxygen into the rubber exhaust head 15. The end of the rubber exhaust head 15 away from the gas guide ring 2 has an inclined surface to withstand external pressure and close the through hole 16 located on the inclined surface, preventing the gas guide chamber 12 from communicating with the culture tank 1 and preventing fermentation broth from entering the gas guide chamber 12. When the pressure piston 7 pressurizes the oxygen, it can open the through hole 16 in this part, thereby spraying out the oxygen. A fixing ring 17 is fixedly connected to the outer wall of the rubber exhaust head 15. The fixing ring 17 is fixedly connected to the inner wall of the gas guide ring 2. After the oxygen reaches the gas guide chamber 12 through the second connecting pipe 13. The air guide chamber 12 allows pressurized oxygen to be evenly distributed within the air guide ring 2. When the pressurizing piston 7 moves to pressurize the oxygen, the rubber exhaust heads 15 at each location can spray oxygen through the connecting holes 16, improving the oxygen diffusion efficiency and ensuring that E. coli in each area of the culture tank can fully contact the oxygen. During the spraying process, the oxygen is fully mixed with the fermentation broth, increasing the oxygen content of the fermentation broth at different locations and ensuring that E. coli can fully reproduce.
[0028] The above are merely preferred embodiments of the present utility model and are not intended to limit the present utility model. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.
Claims
1. A fermentation vessel for culturing Escherichia coli, comprising a culture tank (1), characterized in that: The inner wall of the culture tank (1) is provided with at least two sets of air guide rings (2) for supplying oxygen to the inside of the culture tank (1). A pressure cylinder (3) is fixedly connected to the upper outer wall of the culture tank (1). A pressure piston (7) is slidably connected inside the pressure cylinder (3) for compressing and pressurizing the oxygen entering the pressure cylinder (3). The pressure cylinder (3) has an air inlet (9) on its side wall. The end of the pressure piston (7) away from the air inlet (9) is fixedly connected to an adjusting cylinder (8). The top of the pressure cylinder (3) away from the culture tank (1) is provided with an electric push rod (6). The power output end of the electric push rod (6) is threadedly connected to the adjusting cylinder (8) to change the position of the adjusting cylinder (8) on the power output end of the electric push rod (6) and change the stroke distance of the pressure piston (7). The gas guide ring (2) has a gas guide cavity (12) inside. A rubber exhaust head (15) is inserted into the inner wall of the gas guide ring (2). A connecting hole (16) is provided through the middle of the rubber exhaust head (15) for discharging high-pressure oxygen in the gas guide cavity (12) into the culture tank (1). A second connecting pipe (13) is connected through the middle of the gas guide ring (2).
2. The Escherichia coli culture and fermentation vessel according to claim 1, characterized in that: The top of the adjusting cylinder (8) facing the electric push rod (6) is located outside the pressure cylinder (3). The pressure piston (7) is located at the upper end of the air inlet (9). When the electric push rod (6) is not working, the pressure piston (7) is located between the air inlet (9) and the adjusting cylinder (8). The end of the adjusting cylinder (8) facing the electric push rod (6) is fixedly connected with a rotating ring.
3. The Escherichia coli culture and fermentation vessel according to claim 1, characterized in that: Both ends of the second connecting pipe (13) are threaded structures. A first connecting pipe (11) is provided between two adjacent air guide rings (2) to control the distance between the two air guide rings (2). The two ends of the first connecting pipe (11) are respectively connected to the second connecting pipe (13) on the two air guide rings (2) to guide the oxygen inside the pressurizing cylinder (3) into each air guide ring (2).
4. The Escherichia coli culture and fermentation vessel according to claim 1, characterized in that: The pressure cylinder (3) is fixedly connected to the top of the culture tank (1) with a vent pipe (4). The top of the vent pipe (4) is threadedly connected to the nearest second connecting pipe (13). The lower end of the second connecting pipe (13) at the lowest position is threadedly connected to a sealing cap, which is used to control the sealing of oxygen introduction into the first connecting pipe (11) and the second connecting pipe (13).
5. The Escherichia coli culture and fermentation vessel according to claim 1, characterized in that: The air guide cavity (12) is arranged around the air guide ring (2), and the second connecting pipe (13) is provided with an exhaust hole (14) at the position of the air guide cavity (12).
6. The Escherichia coli culture and fermentation vessel according to claim 1, characterized in that: The end of the connecting hole (16) facing the air guide chamber (12) is a trumpet-shaped structure, which facilitates pressurized oxygen to enter the rubber exhaust head (15). The end of the rubber exhaust head (15) away from the air guide ring (2) is an inclined surface, which is used to withstand external pressure and close the connecting hole (16) located on the inclined surface.
7. The Escherichia coli culture and fermentation vessel according to claim 1, characterized in that: The outer wall of the rubber exhaust head (15) is fixedly connected to a fixing ring (17), and the fixing ring (17) is fixedly connected to the inner wall of the air guide ring (2).
8. The Escherichia coli culture and fermentation vessel according to claim 1, characterized in that: The end of the pressurizing cylinder (3) away from the culture tank (1) is fixedly connected to a support frame (5). The support frame (5) has a U-shaped structure. The electric push rod (6) is fixedly connected to the middle part of the support frame (5). A one-way valve (10) is fixedly connected to the outer wall of the pressurizing cylinder (3) at the air inlet (9).