Full-automatic fluorescent probe experimental equipment

Abstract

The utility model provides full-automatic fluorescent probe experimental equipment, and belongs to the technical field of experimental equipment. Comprising a conveying part used for conveying cover glass and assembled glass slides and cover glass; the stripping part is stably mounted on the conveying part, and the stripping part is used for automatically stripping the cover glass and placing the stripped cover glass on the conveying part; the liquid dropping part is stably mounted right above the conveying part, and the liquid dropping part is used for automatically dropping a DAPI diluent to the cover glass. Through cooperation of the stripping part, the conveying part, the liquid dropping part, the assembling part, the transmission part and other structures, the purpose of full automation of the assembling process of the glass slide and the cover glass is achieved, and through cooperation of a baffle, a spring, an inclined wedge block and an extrusion rod in the assembling frame, stable fixing of the glass slide in the attaching process of the glass slide and the cover glass is achieved.

Description

TECHNICAL FIELD

[0001] The utility model relates to experimental equipment technical field, especially a kind of full-automatic fluorescent probe experimental equipment. BACKGROUND

[0002] Fluorescent probe technology plays a vital role in the field of life science, medicine, material science and other fields;Through fluorescent probe, biological molecules, cell structure and other specific markers and detection can be carried out, which provides a powerful means for in-depth study of life phenomena and disease mechanism;With the continuous development of science and technology, the requirement of fluorescent probe experiment is increasing, and more efficient and accurate experimental equipment is urgently needed to meet the needs of scientific research and clinical application.

[0003] However, in the prior art, the assembly of the glass slide and the cover glass often needs to be operated manually;Such manual assembly process not only increases the complexity of operation, makes the experimental process cumbersome, consumes a lot of time and manpower;Moreover, due to the uncontrollability of human factors, it is easy to make mistakes;For example, when placing the cover glass, it may not be accurate in position and angle deviation, and when fixing the glass slide and the cover glass, it may not be uniform in force, which may affect the accuracy of the experimental results and cannot guarantee the reliability and stability of the experiment. SUMMARY

[0004] The technical problem to be solved by the utility model is to provide a full-automatic fluorescent probe experimental equipment to solve the technical problem that the assembly of the glass slide and the cover glass in the existing fluorescent probe experiment relies on manual operation, which leads to complex operation, easy mistakes and affects the accuracy of experimental results;And when the glass slide and the cover glass are attached, the glass slide is easy to move in the assembly frame, which affects the stability of the attachment.

[0005] Technical solution: To achieve the above object, the utility model discloses a full -automatic fluorescent probe experiment equipment, comprising: conveying part, for conveying cover glass and the assembled glass slide and cover glass;Peeling part, stably installed on the conveying part, and the peeling part is used for automatic peeling cover glass, and the cover glass after peeling is placed on the conveying part;Liquid drop part, stably installed on the conveying part directly above, the liquid drop part is used for adding DAPI diluent to cover glass automatically;Assembly part, stably installed on the conveying part, the assembly part is used for automatic assembly glass slide and cover glass;Transmission part, stably installed on the conveying part, and the transmission part height is adapted with assembly part, and the transmission part is used for transmission glass slide, to make glass slide enter assembly part;The peeling part, liquid drop part and assembly part are arranged in proper order along the conveying direction of conveying part, wherein the running speed of conveying part should be moderate, both guarantee efficient conveying, and cannot be too fast and cause glass slide displacement or damage;The liquid drop part can be composed of liquid storage tank and dropper, and the liquid amount of each drop is ensured accurate through accurate control device;The speed of transmission part should be matched with conveying part, and ensure that glass slide can enter assembly part in time.

[0006] In further embodiment, the adjusting part is stably installed on the conveying part, and the adjusting part is between the peeling part and the liquid drop part, and the adjusting part is used for adjusting the position of cover glass on the conveying part, wherein the adjusting part can be composed of pushing device and sensor, the sensor detects the position of cover glass, and the pushing device adjusts the position of cover glass according to the detection result;The adjusting accuracy of adjusting part should be high, to ensure that cover glass can accurately reach the liquid drop part and the assembly part.

[0007] In further embodiment, the transmission belt is in the transmission part, and the transmission belt is used for transmission glass slide;The limiting block is above the transmission belt, and the limiting block is stably connected in the transmission part, and the limiting block is used for glass slide position limitation;The push rod is stably installed on the transmission part, and the push rod telescopic end is stably connected with the push plate, and the push plate bottom is parallel with the transmission belt upper surface, and the push rod is used for pushing glass slide into assembly part, wherein the transmission belt should have certain friction, to ensure that glass slide does not slide in the transmission process;The position of limiting block should be accurate, to ensure that glass slide can accurately reach the push position;The push rod can be electric push rod or pneumatic push rod, and the material of push plate should be soft, to avoid causing damage to glass slide.

[0008] In another embodiment, the support frame is stably connected to the conveying part; the plurality of extrusion plates are stably connected to the support frame; the plurality of telescopic rods are stably connected to the support frame; the plurality of connecting blocks are stably connected to the telescopic rods; and the assembly frame is stably rotatably connected between the connecting blocks. The assembly frame is below the extrusion plates and is used to hold the glass slides so that the glass slides are attached to the cover glasses. The support frame should have sufficient strength and stability to support each component of the assembly part. The connecting blocks should have sufficient strength to connect the telescopic rods and the assembly frame. The size of the assembly frame should be compatible with the glass slides and the cover glasses to ensure accurate assembly.

[0009] In another embodiment, the two push plates are stably connected to the two sides of the bottom of the assembly frame, respectively. The push plates are used to support the glass slides. The push plates can be flat plates that can stably support the glass slides.

[0010] In another embodiment, the driving motor is stably installed on the connecting blocks. The output end of the driving motor is stably connected to the assembly frame. The driving motor is used to drive the rotation of the assembly frame. The driving motor should have sufficient torque to stably drive the rotation of the assembly frame.

[0011] In another embodiment, the plurality of baffles are slidably connected in the assembly frame. The baffles are used to fix the glass slides. The material of the baffles should have a certain elasticity to avoid damaging the glass slides.

[0012] In another embodiment, the plurality of springs are stably installed in the assembly frame. The springs are used for the reset of the baffles. The plurality of inclined wedge blocks are slidably connected in the assembly frame. The inclined wedge blocks are used to extrude the baffles so that the baffles are retracted into the assembly frame. The extrusion rod is stably connected to the inclined wedge blocks and penetrates the assembly frame. The extrusion rod is directly below the extrusion plate. The extrusion rod is used to drive the movement of the inclined wedge blocks. The spring coefficient should be moderate to achieve the reset of the baffles and not cause excessive pressure on the glass slides. The surface of the inclined wedge block should be smooth to avoid jamming during sliding. The material of the extrusion rod should be strong to withstand external forces.

[0013] In another embodiment, the distance between the two adjacent telescopic rods near the conveying part side of the support frame is greater than the width of the glass slide. The two adjacent telescopic rods are symmetric along the horizontal center line of the assembly frame. The distance should be slightly larger than the width of the glass slide to avoid jamming.

[0014] In a further embodiment, the thickness of the support plate is less than the thickness of the cover glass, and the support plate does not interfere with the cover glass, wherein the thickness of the support plate is strictly controlled to ensure that there is sufficient clearance between the support plate and the cover glass; and the support plate does not affect the fitting of the cover glass and the slide during the assembly process.

[0015] Beneficial effects: 1. The cover glass is automatically peeled off by the peeling part and placed on the conveying part, the droplet part automatically adds DAPI diluent to the cover glass, the conveying part conveys the slide to a position matched with the assembly part, the push rod pushes the slide into the assembly part, and the cooperation of the structures in the assembly part realizes the full automation of the assembly process of the slide and the cover glass; The purpose of completing the assembly without manual intervention is achieved, the operation complexity is effectively reduced, the errors caused by manual operation are reduced, and the accuracy of experimental results is improved.

[0016] 2. The cooperation of the baffle, spring, wedge block and extrusion rod in the assembly frame, and the cooperation of the telescopic rod and extrusion plate on the support frame realizes the stable fixation of the slide during the fitting of the slide and the cover glass; The purpose of ensuring the stability of the slide and the cover glass during fitting is achieved; when the slide needs to enter the assembly frame, the extrusion rod is subjected to the action of the extrusion plate, drives the wedge block to move, and makes the baffle retract into the assembly frame, thereby providing space for the slide to enter the assembly frame smoothly; and when the assembly frame rotates, the assembly frame moves downward to extrude the extrusion rod by means of the conveying part, thereby driving the wedge block to move and making the baffle retract into the assembly frame again, so as to release the fixation of the slide by the baffle, so that the slide falls on the conveying part and is conveyed to the next link. BRIEF DESCRIPTION OF DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or the prior art description. Obviously, the drawings in the following description are only some embodiments of the present application, and those skilled in the art can also obtain other drawings according to these drawings without creative labor.

[0018] Figure 1 The structure of the present application is shown in the figure.

[0019] Figure 2 The main structure of the present application is shown in the figure. Figure 1 The main structure of the present application is shown in the figure.

[0020] Figure 3 The structure of the assembly frame is shown in the figure.

[0021] Figure 4 The structure of the connecting block is shown in the figure.

[0022] Figure 5 The structure of the telescopic rod is shown in the figure.

[0023] Figure 6 Structure diagram of the structure at A of

[0024] Figure 7 Structure diagram of the structure at B of Figure 4

[0025] Figure 8 Structure diagram of the structure at B of Figure 5

[0026] Figure 9 Structure diagram of the structure at C of Figure 6

[0027] The reference signs in the drawings are as follows: 1, conveying part; 2, stripping part; 3, adjusting part; 4, droplet part; 5, assembling part; 501, support frame; 502, extrusion plate; 503, telescopic rod; 504, connecting block; 505, assembling frame; 5051, supporting plate; 6, transmission part; 601, transmission belt; 602, limiting block; 603, pushing rod; 7, driving motor; 8, baffle; 9, inclined wedge block; 901, extrusion rod; 10, spring. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical scheme and advantages of the utility model clearer, the technical scheme in the utility model is described clearly and completely, obviously, the described embodiments are part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the protection scope of the utility model.

[0029] The embodiment of the application provides a kind of full-automatic fluorescent probe experimental equipment, solve the existing fluorescent probe experiment in slide and cover glass assembly rely on manual operation, leading to complex operation, prone to error and affect experimental result accuracy;And when slide and cover glass are pasted, slide is easy to move in assembling frame, thereby affecting the stability of pasting.The actual use, realize the automatic assembly of slide and cover glass, reduce the complexity of operation, reduce the error, improve the accuracy of experimental results, while ensuring the stability when slide and cover glass are pasted.

[0030] In order to better understand the above technical scheme, the above technical scheme will be described in detail in conjunction with the drawings and specific embodiments of the specification.

[0031] Refer to Figures 1-9 ​​​The utility model provides an automatic fluorescent probe experiment equipment, it includes: conveying unit 1 is used for conveying cover glass and assembled cover glass and cover glass, stripping unit 2 is stably installed on conveying unit 1, and the stripping unit 2 is used for automatic stripping cover glass, and places the cover glass after stripping on conveying unit 1, drop liquid part 4 is stably installed on conveying unit 1 directly above, and drop liquid part 4 is used for automatic drop liquid to cover glass DAPI diluent, assembly part 5 is stably installed on conveying unit 1, and assembly part 5 is used for automatic assembly cover glass and cover glass, transmission part 6 is stably installed on conveying unit 1, and transmission part 6 height is adapted with assembly part 5, and transmission part 6 is used for transmission cover glass, to make cover glass enter assembly part 5, and the stripping unit 2, drop liquid part 4 and assembly part 5 are sequentially arranged along the conveying direction of conveying unit 1.

[0032] Through the cooperation of conveying unit 1, stripping unit 2, drop liquid part 4, assembly part 5 and transmission part 6, the automatic stripping, conveying of cover glass, the automatic drop liquid of DAPI diluent, the transmission of cover glass and the automatic assembly effect of cover glass and cover glass are realized.

[0033] Adjusting part 3 is stably installed on conveying unit 1, and the adjusting part 3 is between stripping unit 2 and drop liquid part 4, and the adjusting part 3 is used for adjusting the position of cover glass on conveying unit 1.

[0034] Through the cooperation of adjusting part 3 and conveying unit 1, the effect of adjusting the position of cover glass on conveying unit 1 is realized, and it is ensured that cover glass can accurately reach the subsequent station.

[0035] Transmission belt 601 is in transmission part 6, and the transmission belt 601 is used for transmission cover glass, limiting block 602 is above transmission belt 601, the limiting block 602 is stably connected in transmission part 6, and the limiting block 602 is used for cover glass position limitation, push rod 603 is stably installed on transmission part 6, and the push rod 603 telescopic end is stably connected with push plate, the push plate bottom is parallel with the upper surface of transmission belt 601, and the push rod 603 is used for pushing cover glass into assembly part 5.

[0036] Through the cooperation of transmission belt 601, limiting block 602 and push rod 603, the effect of stable transmission, position limitation and accurate push into assembly part 5 of cover glass in transmission part 6 is realized.

[0037] Support frame 501, stable connection in conveying part 1;Extrusion plate 502, provided with a plurality of, a plurality of said extrusion plate 502 stable connection in support frame 501 telescopic rod 503, provided with a plurality of, and a plurality of said telescopic rod 503 stable connection in support frame 501;Connecting block 504, provided with a plurality of, and a plurality of said connecting block 504 stable connection in telescopic rod 503 telescopic end;Assembly frame 505, stable rotation is connected between connecting block 504, the said assembly frame 505 is below the extrusion plate 502, the said assembly frame 505 for holding slide, so that the slide and cover glass adhere.

[0038] Through the cooperation of support frame 501, extrusion plate 502, telescopic rod 503, connecting block 504 and assembly frame 505, the stable support of assembly frame 505 and the space for the adhesion of slide and cover glass are realized.

[0039] The supporting plate 5051 is provided with two, and the two said push plate is stable connection in the bottom of the two sides of assembly frame 505, the said supporting plate 5051 for supporting slide.

[0040] Through the cooperation of supporting plate 5051 and assembly frame 505, the effect of supporting slide is realized, and the stable placement of slide in assembly frame 505 is ensured.

[0041] The driving motor 7 is stably installed on the connecting block 504, and the output end of the driving motor 7 is stably connected with the assembly frame 505, and the driving motor 7 is used for driving the assembly frame 505 to rotate.

[0042] Through the cooperation of driving motor 7, connecting block 504 and assembly frame 505, the effect of driving assembly frame 505 to rotate is realized, which provides conditions for subsequent operation.

[0043] The baffle 8 is provided with a plurality of, and a plurality of said baffle 8 is connected in the assembly frame 505, and the said baffle 8 is used for slide fixing.

[0044] Through the cooperation of baffle 8 and assembly frame 505, the fixing effect of slide is realized, which prevents the slide from moving in the assembly process.

[0045] The spring 10 is provided with a plurality of, and a plurality of spring 10 is stably installed in the assembly frame 505, and the spring 10 is used for baffle 8 reset;The inclined wedge block 9 is provided with a plurality of, and a plurality of said inclined wedge block 9 is connected in the assembly frame 505, and the said inclined wedge block 9 is used for extruding baffle 8, so that the baffle 8 is retracted into the assembly frame 505;Extrusion rod 901, stable connection in inclined wedge block 9, and the said extrusion rod 901 penetrates the assembly frame 505, the said extrusion rod 901 is below the extrusion plate 502, the said extrusion rod 901 is used for driving the inclined wedge block 9 to move.

[0046] Through the cooperation of the spring 10, the inclined wedge block 9, the extrusion rod 901 and the assembly frame 505, automatic expansion and contraction control of the baffle 8 is realized, thereby realizing the fixing and releasing effect of the slide glass.

[0047] The distance between the two adjacent telescopic rods 503 on the support frame 501 near the transmission part 6 is greater than the width of the slide glass, and the two adjacent telescopic rods 503 are symmetric along the horizontal center line of the assembly frame 505.

[0048] Through the specific distance setting of the adjacent telescopic rods 503 and the cooperation with the assembly frame 505, the effect of facilitating the slide glass to smoothly enter the assembly frame 505 is realized.

[0049] The thickness of the supporting plate 5051 is less than the thickness of the cover glass, and the supporting plate 5051 does not interfere with the cover glass.

[0050] Through the thickness relationship setting of the supporting plate 5051 and the cover glass, the effect that the supporting plate 5051 does not interfere with the cover glass is realized, ensuring the smooth progress of the assembly process.

[0051] In the use process, first, the peeling part 2 automatically peels the cover glass and places it on the conveying part 1, and the conveying part 1 starts to convey the cover glass; when the cover glass reaches the adjusting part 3, the adjusting part 3 adjusts its position on the conveying part 1; then, the cover glass is conveyed to below the droplet part 4, and the droplet part 4 automatically adds DAPI diluent to the cover glass; at the same time, the slide glass is conveyed on the transmission belt 601 of the transmission part 6, and the limiting block 602 limits the position of the slide glass; when the slide glass reaches the specified position, the push rod 603 pushes the push plate to push the slide glass into the assembly frame 505 of the assembly part 5; in this process, the distance between the two adjacent telescopic rods 503 near the transmission part 6 is greater than the width of the slide glass, facilitating the slide glass to enter the assembly frame 505; after the slide glass enters the assembly frame 505, the supporting plate 5051 supports the slide glass; the baffle 8 in the assembly frame 505 is extended under the action of the spring 10 and the inclined wedge block 9, fixing the slide glass; then, the telescopic rod 503 drives the assembly frame 505 to move up and down, when the assembly frame 505 moves up, the extrusion plate 502 will extrude the extrusion rod 901 to make the inclined wedge block 9 move down, and the baffle 8 is retracted into the assembly frame 505 for the slide glass to enter; when the assembly frame 505 moves down, the spring 10 makes the baffle 8 protrude again to fix the slide glass; then, the telescopic rod 503 continues to move down, so that the slide glass contacts with the cover glass, and then the telescopic rod 503 rises, the drive motor 7 drives the assembly frame 505 to rotate 180 degrees, so that the extrusion rod 901 faces downward; the telescopic rod 503 descends again, the extrusion rod 901 contacts with the conveying part 1 and is extruded into the assembly frame 505, the inclined wedge block 9 moves to make the baffle 8 retract into the assembly frame 505, releasing the fixing of the slide glass, and the slide glass falls on the conveying part 1, and the conveying part 1 conveys the assembled slide glass and cover glass to the next link.

[0052] The positioning device, DAPI diluent conveying device, protection device and driving power source required in the above description are all prior art and are non-essential technical features in the present application, so they are not described or drawn in the documents and drawings of the present application; the figures shown in the drawings are example figures, and the purpose is only to more intuitively show the key structure and connection relationship of the full-automatic fluorescent probe experiment device; in actual application, the appearance and size of the device can be adjusted and optimized according to specific needs.

[0053] In summary, compared with the prior art, the present application has the following beneficial effects: full automation of slide and cover glass assembly is achieved, manual operation is no longer needed, and the operation complexity is reduced; in the process of adhering the slide and the cover glass, the cooperation of the baffles, springs, wedge blocks and extrusion rods in the assembly frame, as well as the synergistic effect of the support frame, telescopic rod and extrusion plate, ensures the stability of the slide in the assembly frame, avoids affecting the stability of the adhesion due to the movement of the slide, and thus improves the accuracy of the experimental results.

[0054] The present application covers any alternative, modification, equivalent method and scheme made in the essence and scope of the present application. In order to enable the public to have a thorough understanding of the present application, specific details are described in the above preferred embodiments of the present application, and the present application can be completely understood without the description of these details for those skilled in the art. In addition, in order to avoid unnecessary confusion of the essence of the present application, well-known methods, processes, procedures, elements and circuits are not described in detail.

[0055] The above is only a preferred embodiment of the present application, and it should be pointed out that for ordinary skilled persons in the technical field, without departing from the principle of the present application, a number of improvements and refinements can be made, and these improvements and refinements should also be considered as the protection scope of the present application.

Claims

1. A fully automated fluorescent probe experimental device, characterized in that, include: The conveying unit (1) is used to convey the cover glass slide and the assembled slide and cover glass slide; The peeling part (2) is stably installed on the conveying part (1), and the peeling part (2) is used to automatically peel off the cover glass and place the peeled cover glass on the conveying part (1); The dripping part (4) is stably installed directly above the delivery part (1). The dripping part (4) is used to automatically drip DAPI diluent onto the coverslip. Assembly unit (5) is stably mounted on conveyor unit (1), and assembly unit (5) is used for automatically assembling and loading glass slides and cover glass slides; The transmission section (6) is stably installed on the conveying section (1), and the height of the transmission section (6) is adapted to the assembly section (5). The transmission section (6) is used to transmit the glass slide so that the glass slide enters the assembly section (5). The peeling section (2), the dripping section (4), and the assembly section (5) are arranged sequentially along the conveying direction of the conveying section (1).

2. The fully automated fluorescent probe experimental device according to claim 1, characterized in that, Also includes: An adjustment part (3) is stably installed on the conveying part (1), and the adjustment part (3) is located between the peeling part (2) and the dripping part (4). The adjustment part (3) is used to adjust the position of the cover glass on the conveying part (1).

3. The fully automated fluorescent probe experimental device according to claim 1, characterized in that, The transmission unit (6) includes: A conveyor belt (601) is located within the conveying section (6), and the conveyor belt (601) is used to convey glass slides; A limiting block (602) is located above the conveyor belt (601). The limiting block (602) is stably connected to the conveyor section (6). The limiting block (602) is used to limit the position of the glass slide. A push rod (603) is stably installed on the transmission section (6). A push plate is stably connected to the telescopic end of the push rod (603). The bottom of the push plate is parallel to the upper surface of the transmission belt (601). The push rod (603) is used to push the glass slide into the assembly section (5).

4. The fully automated fluorescent probe experimental device according to claim 3, characterized in that, Assembly unit (5) includes: The support frame (501) is stably connected to the conveying unit (1); Multiple extrusion plates (502) are provided, and the multiple extrusion plates (502) are stably connected to the support frame (501). Multiple telescopic rods (503) are provided, and the multiple telescopic rods (503) are stably connected to the support frame (501); Multiple connecting blocks (504) are provided, and the multiple connecting blocks (504) are stably connected to the telescopic end of the telescopic rod (503); An assembly frame (505) is stably rotatably connected between connecting blocks (504). The assembly frame (505) is located below the extrusion plate (502). The assembly frame (505) is used to hold a glass slide so that the glass slide fits against the cover glass.

5. The fully automated fluorescent probe experimental device according to claim 4, characterized in that, Also includes: Two support plates (5051) are provided, and the two support plates are stably connected to the two sides of the bottom of the assembly frame (505). The support plates (5051) are used to support glass slides.

6. The fully automated fluorescent probe experimental device according to claim 4, characterized in that, Also includes: A drive motor (7) is stably mounted on a connecting block (504). The output end of the drive motor (7) is stably connected to the assembly frame (505). The drive motor (7) is used to drive the assembly frame (505) to rotate.

7. The fully automated fluorescent probe experimental device according to claim 4, characterized in that, Also includes: Multiple baffles (8) are provided, and the multiple baffles (8) are slidably connected in the assembly frame (505). The baffles (8) are used to fix the glass slides.

8. The fully automated fluorescent probe experimental device according to claim 4, characterized in that, Also includes: A plurality of springs (10) are provided, and the plurality of springs (10) are stably installed in the assembly frame (505). The springs (10) are used for the resetting of the baffle (8). Multiple wedge blocks (9) are provided, and the multiple wedge blocks (9) are slidably connected in the assembly frame (505). The wedge blocks (9) are used to press the baffle (8) so that the baffle (8) retracts into the assembly frame (505). The extrusion rod (901) is stably connected to the wedge block (9), and the extrusion rod (901) passes through the assembly frame (505). The extrusion rod (901) is located directly below the extrusion plate (502). The extrusion rod (901) is used to drive the wedge block (9) to move.

9. The fully automated fluorescent probe experimental device according to claim 4, characterized in that: The distance between two adjacent telescopic rods (503) on the support frame (501) near the transmission part (6) is greater than the width of the glass slide, and the two adjacent telescopic rods (503) are symmetrical along the horizontal center line of the assembly frame (505).

10. The fully automated fluorescent probe experimental device according to claim 5, characterized in that: The thickness of the support plate (5051) is less than the thickness of the cover glass, and the support plate (5051) and the cover glass do not interfere with each other.

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