Tissue culture bottle for assisting tissue culture bag in use

Abstract

The utility model belongs to the technical field of plant tissue culture devices, and particularly relates to a tissue culture bottle for assisting a tissue culture bag in use, which comprises a bottle body and a cover body, the cover body is detachably connected with the bottle body, the diameter of the bottle body is gradually increased from bottom to top, a supporting part is arranged on the inner wall of the bottle body, and the tissue culture bag is arranged in the bottle body. A hard ring is arranged on the tissue culture bag, a groove is formed in the bottom of the hard ring, and the hard ring is arranged on the supporting part. The supporting part is arranged on the inner wall of the bottle body and matched with the tissue culture bag to support the tissue culture bag, the tissue culture bag is sealed by sealing the bottle body through the cover body, heat sealing at the opening of the bag body is not needed, the problem that the tissue culture bag is prone to collapse is solved, filling of a liquid culture medium in production is facilitated, and production efficiency is improved. The filling effect of the culture medium and the growth space of tissue culture seedlings are not extruded. The tissue culture bag is a disposable tissue culture bag, so that the tissue culture bottle can be recycled without being cleaned.

Description

TECHNICAL FIELD

[0001] The utility model belongs to plant tissue culture device technical field, concretely relates to a tissue culture bottle for assisting tissue culture bag use. BACKGROUND

[0002] In the plant tissue culture industry, transparent glass bottles or plastic bottles resistant to high-temperature and high-pressure steam sterilization (121 DEG C) are often used as tissue culture bottles to provide a sterile culture environment for tissue culture seedlings. The tissue culture bottle usually uses a cover with a breathable filter membrane or a rubber plug with a cotton plug filling the air hole to achieve the function of allowing the tissue culture bottle to breathe and isolate external bacteria and microorganisms. However, the cost of glass bottles or plastic bottles is high, and before being reused, the culture medium in the bottle needs to be poured out and cleaned, which costs a lot of manpower. After being cleaned several times, scratches inevitably appear on the surface of the bottle, which greatly reduces the light transmittance and directly affects the growth quality of the tissue culture seedlings.

[0003] To solve the above problems, the applicant has filed a utility model patent with the application number 202521783921.X, which discloses a plant tissue culture device, comprising: a tray including a frame and a hole disc, the hole disc is placed on the frame, and a plurality of circular hole grooves are arranged on the hole disc; a tissue culture bag placed in the hole groove, including a bag body, the cross section of the bag body is circular, the diameter of the bag body gradually increases from bottom to top, a hard ring is arranged at the top of the bag body, a groove is opened at the bottom of the hard ring, at least three lugs are arranged on the outer wall of the hard ring, and a breathable hole is opened on the bag body; a tissue culture rack. However, the tissue culture bag in the device is heat-sealed by the identification ring line at the top during use, the surface of the plastic bag body is often deformed and uneven after high-pressure sterilization, the sealing effect is poor; and the space at the top of the tissue culture bag is squeezed and folded after sealing, which reduces the growth space of the tissue culture seedlings; the tissue culture bag is discarded after being used once, and the filter membrane is also wasted because it is integrated with the tissue culture bag. CONTENT OF THE UTILITY MODEL

[0004] The utility model aims to provide a tissue culture bottle for assisting tissue culture bag use, mainly solves the problem that the tissue culture bag is not sealed after sealing and the deformation affects the cultivation operation in the background technology, and solves the problem of economic waste caused by the use of filter membranes only once.

[0005] To achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A tissue culture bottle for assisting tissue culture bag use, comprising a bottle body and a cover, the cover and the bottle body are detachably connected, the diameter of the bottle body gradually increases from bottom to top, a support part is arranged on the inner wall of the bottle body, a tissue culture bag is arranged in the bottle body, a hard ring is arranged on the tissue culture bag, a groove is arranged at the bottom of the hard ring, and the hard ring is arranged on the support part;

[0007] The cover body is provided with a clamping ring distributed along the edge of the through hole, and a sealing ring is detachably arranged on the clamping ring.

[0008] Preferably, the support part is a support ring.

[0009] Preferably, the support ring extends inwardly in an inclined manner to form a V-shaped groove with the inner wall of the bottle body.

[0010] Preferably, the support part is a bent groove.

[0011] Preferably, the bottle body is bent downwardly first and then upwardly to form a bent groove near the top, and the bent groove is divided into an inner bent part and an outer bent part, and the inner bent part corresponds to the groove at the bottom of the hard ring.

[0012] Preferably, the support part is a plurality of hooks.

[0013] Preferably, a hanging rod is arranged on the hook.

[0014] Preferably, the sealing ring is provided with an annular groove corresponding to the clamping ring, and the sealing ring is clamped on the clamping ring.

[0015] Preferably, the annular groove has an arc-shaped cross section, and the clamping ring has an upper part and a lower part, the upper part has an arc shape, and the lower part is integrally formed with the cover body.

[0016] Preferably, the through hole is circular or square.

[0017] The beneficial effects of the utility model are as follows:

[0018] The inner wall of the bottle body is provided with a support part, which cooperates with the tissue culture bag to support the tissue culture bag, and the tissue culture bag is sealed by sealing the bottle body with the cover body, so that the problem of easy collapse of the tissue culture bag is solved, and the filling of the liquid medium in production is facilitated, and the filling effect of the medium is ensured.

[0019] The cooperation of the cover body, the sealing ring and the sealing film eliminates the operation of twice heat sealing of the tissue culture bag, realizes the functions of isolating external microorganisms and maintaining the air permeability of the bottle, and increases the growth space of the tissue culture seedlings.

[0020] The detachable connection of the sealing ring and the clamping ring facilitates the replacement of the damaged filter film, the whole cover body does not need to be replaced, and the cover body can be used repeatedly.

[0021] The tissue culture bag does not need to have a filter film structure, the filter film is separated from the tissue culture bag, the tissue culture bag is discarded after use, the filter film does not need to be arranged on the tissue culture bag, and the cost is reduced.

[0022] When the medium of the tissue culture seedling is replaced, the disposable tissue culture bag is discarded, the tissue culture bottle does not need to be cleaned, and the labor cost is saved;

[0023] The bottle body is designed to be wide at the top and narrow at the bottom, which is beneficial to storage, reduces the occupied space, and facilitates transportation. BRIEF DESCRIPTION OF DRAWINGS

[0024] The drawings accompanying the specification of this application form a part thereof, serve to provide further understanding of the present application, and together with the description of the exemplary embodiments of the present application, serve to explain the present application, and do not constitute an improper limitation on the present application. In the drawings:

[0025] Figure 1 It is a perspective view of a tissue culture bottle for assisting a tissue culture bag according to Embodiment 1 of the present application;

[0026] Figure 2 It is a perspective view of a tissue culture bottle for assisting a tissue culture bag according to Embodiment 1 of the present application, from which a sealing ring and a sealing film are removed;

[0027] Figure 3 It is a structural schematic view of a tissue culture bottle for assisting a tissue culture bag according to Embodiment 1 of the present application;

[0028] Figure 4 It is Figure 3 A partial enlarged view of A;

[0029] Figure 5 It is a structural schematic view of a tissue culture bottle for assisting a tissue culture bag according to Embodiment 2 of the present application;

[0030] Figure 6 It is Figure 5 A partial enlarged view of B;

[0031] Figure 7 It is a structural schematic view of a tissue culture bottle for assisting a tissue culture bag according to Embodiment 3 of the present application;

[0032] Figure 8 It is Figure 7 A partial enlarged view of C.

[0033] Wherein: 1, bottle body; 2, cover body; 3, sealing ring; 4, sealing film; 5, clasp ring; 11, supporting ring; 12, hard ring; 13, hanging ear; 14, bending groove; 15, hook; 16, hanging rod. DETAILED DESCRIPTION

[0034] The present application will be described in detail below with reference to the accompanying drawings and in combination with the embodiments. It should be noted that the embodiments in the present application and the features in the embodiments can be combined with each other without conflict.

[0035] The following detailed description is exemplary and intended to provide further detailed explanation of the present invention. Unless otherwise specified, all technical terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains. The terminology used in this invention is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to the present invention.

[0036] In the description of this utility model, it should be understood that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined with "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more. In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0037] Example 1

[0038] like Figures 1-4 As shown, a tissue culture bottle used as an auxiliary tissue culture bag includes a bottle body 1 and a cap 2, wherein the cap 2 and the bottle body 1 are detachably connected.

[0039] The diameter of the bottle body 1 gradually increases from bottom to top. The bottle body 1 is made of transparent material, specifically glass or plastic, and is resistant to high temperature and high pressure steam sterilization.

[0040] A support ring 11 is provided on the inner wall of the bottle body 1. The support ring 11 and the bottle body 1 are integrally formed. The support ring 11 extends inward at an angle and forms a V-shaped groove with the inner wall of the bottle body 1. When in use, the tissue culture bag is placed in the bottle body 1. A rigid ring 12 is provided on the tissue culture bag. The bottom groove of the rigid ring 12 is engaged with the support ring 11 to support the tissue culture bag and make the tissue culture bag fully supported.

[0041] The middle part of the cover 2 is provided with a through hole, which is circular or square, for air permeation;

[0042] The cover 2 is provided with a snap ring 5, which is distributed along the edge of the through hole;

[0043] The snap ring 5 is connected with a sealing ring 3, which is provided with an annular groove at the bottom corresponding to the snap ring 5, and the sealing ring 3 is clamped on the snap ring 5, and a sealing film 4 is arranged between the sealing ring 3 and the snap ring 5, and the sealing film 4 is provided with an air permeable filter film corresponding to the position of the through hole;

[0044] As a specific example of the above embodiment, the sealing film 4 is a plastic film, and the air permeable filter film is hot-pressed on the sealing film 4;

[0045] As a specific example of the above embodiment, the cross section of the annular groove is arc-shaped and is an optimal arc, and the cross section of the snap ring 5 is divided into upper and lower parts, the upper part is an optimal arc, and the lower part is integrally formed with the cover 2, and in use, the plastic film with filter film is placed on the bottle cap, the filter film is above the through hole, the groove of the sealing ring 3 is aligned with the buckle on the bottle cap, and the sealing is completed by pressing downward.

[0046] As a preferred example of the above embodiment, the sealing ring 3 is made of material or latex.

[0047] As a preferred example of the above embodiment, the cover 2 is made of plastic material and can withstand high temperature and high pressure steam sterilization.

[0048] As a preferred example of the above embodiment, the cover 2 is connected with the bottle body 1 through threads.

[0049] As a preferred example of the above embodiment, the cover 2 is connected with the bottle body 1 through a buckle.

[0050] The specific operation steps are as follows:

[0051] 1. Prepare tissue culture bottles and tissue culture bags

[0052] Place the tissue culture bag in the bottle body 1, and the hard ring 12 of the tissue culture bag is hung on the hook 15 on the inner wall of the tissue culture bottle, and the bottom of the bag body is attached to the bottom of the bottle body 1.

[0053] 2. Fill the medium into the tissue culture bag

[0054] Fill the prepared and dissolved liquid medium into the tissue culture bag, and fill 50-100 mL of liquid medium into each bag.

[0055] 3. Seal the tissue culture bottle

[0056] Place the sealing film 4 on the cap 2 and connect it with the sealing ring 3 and the retaining ring 5 at the top of the cap 2 to seal and fix the sealing film 4. Make sure to place the filter membrane at the through hole of the cap 2. After assembling, seal the cap 2 with the bottle 1.

[0057] 4. Autoclave the culture medium.

[0058] After filling the culture medium bottles, seal them and sterilize them in an autoclave at 121°C for 20-25 minutes.

[0059] 5. The culture medium cools and solidifies.

[0060] The sterilized culture medium is transported to the inoculation room and allowed to cool and solidify naturally overnight before use.

[0061] 6. Inoculation procedure for tissue culture seedlings

[0062] Open the laminar flow hood and disinfect it beforehand. Place the tissue culture bottle inside the laminar flow hood, unscrew cap 2, and invert cap 2 onto the workbench. Then, inoculate the prepared tissue culture seedlings into the culture medium. Finally, replace cap 2 on bottle 1. Transport the inoculated tissue culture bottle to the culture room for cultivation.

[0063] 7. Change the culture medium for tissue culture seedlings

[0064] When transferring tissue culture seedlings that have grown for a period of time to a new culture medium, use sterile forceps in a laminar flow hood to remove the seedlings from the tissue culture bottle and transfer them to a new culture bottle containing fresh medium. The original tissue culture bottle only needs to have its culture bag removed and discarded. The bottle body and cap are then reused. The filter membrane may become damaged after multiple uses and needs to be replaced promptly to ensure effective filtration of microorganisms.

[0065] Example 2

[0066] like Figures 5-6 As shown, the bottle body 1 is first bent downwards near the top, and then bent upwards to form a bending groove 14. The bending groove 14 is divided into an inner bending part and an outer bending part. The inner bending part corresponds to the bottom groove of the rigid ring 12. The rigid ring 12 is placed on the inner bending part, and the outer edge of the rigid ring 12 is placed inside the outer bending groove 14. The hanging ear 13 is folded and stored outside the rigid ring 12. The design of the bending groove 14 can reduce the weight of the bottle body and make the shape of the bottle body 1 below the bending groove 14 fit the tissue culture bag better, providing better support for the tissue culture bag and avoiding gaps between the tissue culture bag and the tissue culture bottle, which could cause deformation of the tissue culture bag and affect the tissue culture effect.

[0067] Example 3

[0068] like Figures 7-8As shown, the bottle body 1 is fixedly provided with a plurality of hooks 15 on the inner wall, the number of the hooks 15 is same as the number of the ears 13, one end of the hook 15 is fixedly connected with the bottle body 1, the other end of the hook 15 is upwardly bent and placed in the groove at the bottom of the hard ring 12, the hook 15 is provided with a hanging rod 16, and the hanging rod 16 is inserted into the ear 13.

[0069] In the description of the present specification, the description of the terms "one embodiment", "example", "specific example" and the like means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are contained in at least one embodiment or example of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0070] As known from common technical knowledge, the present application can be realized by other embodiments without departing from the spirit or essential characteristics thereof. Therefore, the above disclosed embodiments are merely illustrative in all aspects, and are not the only ones. All changes within the scope of the present application or within the scope equivalent to the present application are included in the present application.

Claims

1. A tissue culture bottle for assisting the use of a tissue culture bag, comprising a bottle body (1) and a cover body (2), the cover body (2) and the bottle body (1) being detachably connected, characterized in that, The diameter of the bottle body (1) gradually increases from bottom to top, a support part is arranged on the inner wall of the bottle body (1), a tissue culture bag is arranged in the bottle body (1), a hard ring (12) is arranged on the tissue culture bag, a groove is arranged on the bottom of the hard ring (12), and the hard ring (12) is arranged on the support part. A through hole is formed in the middle of the cover body (2), a clasp ring (5) is arranged on the cover body (2), the clasp ring (5) is distributed along the edge of the through hole, a sealing ring (3) is detachably arranged on the clasp ring (5), a sealing film (4) is arranged between the sealing ring (3) and the clasp ring (5), and a breathable filter film is arranged on the sealing film (4) corresponding to the position of the through hole.

2. The tissue culture bottle for use with the supplemental tissue culture bag of claim 1, wherein, The support part is a support ring (11).

3. The tissue culture bottle for use with the auxiliary tissue culture bag of claim 2, wherein, The support ring (11) extends inwardly and obliquely, and forms a V-shaped groove between the inner wall of the bottle body (1).

4. The tissue culture bottle for use with the supplemental tissue culture bag of claim 1, wherein, The support part is a bent groove (14).

5. The tissue culture bottle for use with the supplemental tissue culture bag of claim 4, wherein, The bottle body (1) is first bent downwardly and then upwardly near the top to form the bent groove (14), the bent groove (14) is divided into an inner bent part and an outer bent part, and the inner bent part corresponds to the groove on the bottom of the hard ring (12).

6. The tissue culture bottle for use with the supplemental tissue culture bag of claim 1, wherein, The support part is a plurality of hooks (15).

7. The tissue culture bottle for use with the supplemental tissue culture bag of claim 6, wherein, A hanging rod (16) is arranged on the hook (15).

8. The tissue culture bottle for use with the supplemental tissue culture bag of claim 1, wherein, An annular groove corresponding to the clasp ring (5) is formed on the bottom of the sealing ring (3), and the sealing ring (3) is clamped on the clasp ring (5).

9. The tissue culture bottle for use with the auxiliary tissue culture bag of claim 8, wherein, The cross section of the annular groove is arc-shaped and is an optimal arc, and the cross section of the clasp ring (5) is divided into an upper part and a lower part, the upper part is an optimal arc, and the lower part is integrally formed with the cover body (2).

10. The tissue culture bottle for use with the supplemental tissue culture bag of claim 1, wherein, The through hole is circular or square.

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