Semi-automatic solution purification device
By using a semi-automatic solution purification device, which utilizes a peristaltic pump and a semi-permeable fixing plate to control the solution flow rate, the problem of difficult flow rate control in existing technologies has been solved, achieving efficient solution separation and purification as well as simple operation.
Patent Information
- Application Number
- CN202423250568.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-27
- Publication Date
- 2025-12-16
- Estimated Expiration
- 2034-12-27
AI Technical Summary
Existing technologies require manual flow rate control in HDEHP-kel-F chromatography column operations, which is difficult to determine quickly and accurately, is time-consuming, and has high operational difficulty, thus affecting separation and purification efficiency.
A semi-automatic solution purification device is adopted, which uses a peristaltic pump to control the flow rate and amount of solution flowing through the color powder. A semi-permeable fixing plate allows the solution to pass through while blocking the color powder, thereby realizing the semi-automatic separation and purification of the solution.
It achieves efficient separation and purification of solutions, reduces operational difficulty, and improves separation and purification effects as well as ease of operation.
Smart Images
Figure CN223668705U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to purification device technical field, concretely relates to a kind of semi-automated solution purification device. BACKGROUND
[0002] The HJ 815-2016 two-(2-ethylhexyl) phosphoric acid extraction chromatography method is suitable for the determination of Sr in water and animal and plant ash. 90 After the sample to be measured is pretreated and the acidity is adjusted, the solution is adsorbed with yttrium through a polytrifluorochloroethylene (kel-F) chromatographic column coated with di-(2-ethylhexyl) phosphoric acid (HDEHP for short), then 1.5 mol / L nitric acid is used to rinse the chromatographic column, elute other adsorbed strontium, cesium, cerium, promethium and other ions except yttrium, and analyze yttrium with 6 mol / L nitric acid in the form of yttrium oxalate precipitation for beta counting and weighing.
[0003] In the preparation of HDEHP-kel-F chromatographic column, the sample solution loading, multiple washing to remove impurities and the final yttrium analysis process, the previous operation needs to manually control the constant flow rate, and different flow rates need to be adjusted at different steps. Such a way is time-consuming, and it is difficult to quickly and accurately determine the constant flow rate, which is not conducive to separation and purification, and the operator needs to keep attention at all times to operate, which increases the operation difficulty of the operator. UTILITY MODEL CONTENT
[0004] To solve the above technical problems, the utility model provides a kind of semi-automated solution purification device, including:
[0005] The first container contains the solution to be separated or the eluent or the eluent;
[0006] The second container is sealedly connected with the outlet end of the first container, and the first fixed sheet is arranged in the second container; the side of the first fixed sheet close to the second container is provided with chromatographic powder; the first fixed sheet has semi-permeability, which can allow the solution to be separated or the eluent or the eluent to pass through, and block the chromatographic powder from passing through;
[0007] The peristaltic pump is sealedly connected with the outlet end of the second container through a hose, and the pressure state in the hose and the second container is changed by the peristaltic pump to control the solution to be separated or the eluent to enter the second container and flow through the chromatographic powder, so that the separated substance in the solution to be separated is attached to the chromatographic powder, or the eluent is washed to the chromatographic powder, or the eluent is eluted to the separated substance in the chromatographic powder.
[0008] Preferably, the second container is provided with a plurality of second containers, each of which is loaded with an equal amount of chromatographic powder, each of which is in communication with the first container, and the outlet end of each of the second containers is correspondingly provided with the hose, and the plurality of hoses are connected in parallel on the peristaltic pump.
[0009] Preferably, the first container is correspondingly provided with a plurality of first containers according to the number of the second containers, and each of the second containers is in communication with the corresponding first container.
[0010] Preferably, the third container is further included, the first container contains the solution to be separated, and the third container contains the eluent or the eluent;
[0011] The second container is sealingly connected to the first container to perform the adsorption separation operation on the solution to be separated, or the second container is sealingly connected to the third container to perform the elution or elution operation on the chromatographic powder.
[0012] Preferably, the second container is sealingly connected to the inlet conduit, the inlet conduit is in communication with the first container and the third container through a three-way valve, and the communication state of the inlet conduit with the first container and the third container is controlled by changing the valve state of the three-way valve.
[0013] Preferably, sealing rubber plugs are provided at the inlet end and the outlet end of the second container, and the outlet end of the first container and the inlet end of the hose pass through the sealing rubber plugs into the second container to achieve sealing connection with the second container.
[0014] Preferably, sealing end covers are provided at the inlet end and the outlet end of the second container, end cover valves are opened on the sealing end covers, and the first container and the hose are connected to the sealing end covers, and the second container is in a communication state or a closed state by opening and closing the end cover valve.
[0015] Preferably, a second fixed sheet is further included, the second fixed sheet is completely the same in material and shape as the first fixed sheet, the second fixed sheet and the first fixed sheet are detachably installed in the second container along the axial direction of the second container, and the chromatographic powder is stored between the second fixed sheet and the first fixed sheet.
[0016] Preferably, the first container includes a loading part and a conduit part, the loading part contains the solution to be separated or the eluent, one end of the conduit part is in communication with the bottom end of the loading part, the other end of the conduit part is connected to the upper end of the second container, and a valve is provided on the conduit part.
[0017] Preferably, a collection bottle is provided at the outlet end of the hose.
[0018] The technical scheme of the utility model is applied, the first container contains the solution to be separated or the elution liquid, the inlet end of the second container is sealingly connected with the first container, the outlet end is sealingly connected with the hose of the peristaltic pump, the outlet end of the second container is provided with a semi-permeable first fixed sheet, the color layer powder is loaded on the side of the first fixed sheet close to the first container, the first fixed sheet can allow the solution to be separated and the elution liquid to pass through and block the color layer powder from passing through. By controlling the peristaltic pump, the flow rate and total amount of the solution to be separated or the elution liquid entering the second container can be controlled, the solution to be separated or the elution liquid of a specified mass enters the second container at a constant rate, and the solution to be separated or the elution liquid passes through the color layer powder at a specified rate, thereby improving the separation and purification effect of the color layer powder on the solution to be separated, and improving the elution effect of the elution liquid on the color layer powder and the elution effect of the elution liquid on the separated substance in the color layer powder. Meanwhile, semi-automation of solution separation and purification is realized, and the operation difficulty of the operator is reduced. BRIEF DESCRIPTION OF DRAWINGS
[0019] Figure 1 is the overall schematic view of the semi-automated solution purification device provided by the utility model embodiment;
[0020] Figure 2 is the internal structure schematic view of the second container of the semi-automated solution purification device provided by the utility model embodiment;
[0021] Figure 3 is the overall schematic view of the semi-automated solution purification device provided by another utility model embodiment;
[0022] Among them, 1, the first container; 11, the loading part; 12, the conduit part; 121, the valve; 2, the second container; 21, the sealing rubber plug; 22, the first fixed sheet; 23, the second fixed sheet; 3, the peristaltic pump; 4, the hose; 5, the collection bottle; 6, the third container; 7, the three-way valve. DETAILED DESCRIPTION
[0023] To make the purpose, technical scheme and advantages of the utility model embodiments clearer, the technical scheme in the utility model embodiments will be described clearly and completely in combination with the drawings in the utility model embodiments. Obviously, the described embodiments are part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the protection scope of the utility model.
[0024] Figure 1It is the whole schematic view of the semi-automatic solution purification device provided by the embodiment of the utility model, Figure 2 It is the internal structure schematic view of the second container of the semi-automatic solution purification device provided by the embodiment of the utility model.
[0025] As Figure 1 With Figure 2 The semi-automatic solution purification device provided by the embodiment of the utility model, including first container 1 and second container 2, the first container 1 is loaded with the solution to be separated or the eluent or the eluate, the inlet end of the second container 2 is sealedly connected with the outlet end of the first container 1, the first fixed sheet 22 is arranged in the second container 2, the color layer powder is loaded on the side of the first fixed sheet 22 close to the first container 1, the first fixed sheet 22 has semi-permeability, can allow the gas-liquid molecules or ions of small volume to pass through, and block the solid particles of large volume from passing through, that is, can allow the solution to be separated and the eluent, eluate to pass through, and block the color layer powder from passing through, the peristaltic pump 3 is arranged in the downstream of the second container 2, the hose 4 is installed on the peristaltic pump 3, and the hose 4 is sealedly connected with the outlet end of the second container 2.
[0026] The first fixed sheet 22 can be made of a porous filter sheet by sintering, and the material can be PTFE, PE, PP or the like, the pore diameter of the holes on the surface is smaller than the particle size of the color layer powder used, and the use requirement of allowing the solution to be separated to pass through and blocking the color layer powder from passing through can be met. For example, in one specific embodiment, the solution to be separated in the first container 1 is a strontium yttrium solution, the color layer powder used is HDEHP, which is a macroporous silicon-based carrier, and the particle size is 50 microns. The pore diameter on the first fixed sheet 22 can be designed as 20 microns, so that the solution to be separated can flow through the color layer powder and the first fixed sheet 22, and the color layer powder cannot pass through the first fixed sheet. Of course, the material and pore diameter of the first fixed sheet can be adaptively adjusted according to the specific components of the solution to be separated and the color layer powder. The above optional embodiment is only used to illustrate the technical scheme of the utility model and is not limited.
[0027] In addition, in the embodiment of the utility model, the first container 1 contains the solution to be separated, the eluent or the eluate, which is only for the case that the elution and washing of the separation process of the solution to be separated are required. If the separated substance in the solution to be separated only needs to be eluted after being adsorbed by the color layer powder to realize the collection of the separated substance, the eluent does not need to be set again. If the separated substance in the solution to be separated still needs other types of solution to process the color layer powder after being adsorbed by the color layer powder, the corresponding solution can be set in the first container 1, and then the peristaltic pump is used to make it enter the second container 2 and flow through the color layer powder. The types of the above solutions and the specific operation process can be adjusted by the actual operator according to the actual situation.
[0028] Since the hose 4, the second container 2 and the first container 1 are integrally sealed, when the peristaltic pump 3 is working, the pressure state in the second container 2 can be changed by changing the pressure state in the hose 4, so that the solution to be separated or the eluent in the first container 1 enters the second container 2. The color layer powder and the first fixed sheet 22 are both porous structures, and the pore size of the first fixed sheet 22 is smaller than the particle size of the color layer powder. Therefore, the solution to be separated can flow through the color layer powder and the first fixed sheet 22, and the separated substance in the solution can be adsorbed by the color layer powder. The color layer powder adsorbed with the separated substance is blocked by the first fixed sheet 22 and continues to stay in the second container 2. Then, the solution in the first container 1 is replaced by the eluent, which is introduced into the second container 2 under the action of the peristaltic pump. When the eluent flows through the first fixed sheet 22 and the color layer powder, the color layer powder adsorbed with the separated substance can be eluted to wash off the impurities other than the separated substance adsorbed on the color layer powder. Finally, the solution in the first container 1 is replaced by the eluent, which can elute the separated substance on the color layer powder to obtain a high-purity solution of the separated substance.
[0029] By controlling the peristaltic pump, the flow rate and total amount of the solution to be separated or the eluent entering the second container can be controlled, so that a specified mass of the solution to be separated or the eluent or the eluent enters the second container at a constant rate, and the solution to be separated or the eluent or the eluent passes through the color layer powder at a specified rate. Therefore, the separation and purification effect of the color layer powder on the solution to be separated is improved, and the elution effect of the eluent on the color layer powder and the elution effect of the eluent on the separated substance in the color layer powder are improved. At the same time, semi-automation of solution separation and purification is realized, and the operation difficulty of the operator is reduced.
[0030] In one specific embodiment, the semi-automatic solution purification device can be used for the purification of yttrium in strontium yttrium solution, wherein the first container 1 can adopt a glass beaker, the strontium yttrium solution is contained in the first container 1, the second container 2 can adopt a color column made of polytrifluorochloroethylene, the first fixed sheet 22 is arranged in the second container 2, the di(2-ethylhexyl) phosphoric acid (abbreviated as HDEHP) is loaded above the first fixed sheet 22, under the action of the peristaltic pump 3, the strontium yttrium solution in the first container enters the second container 2 and flows through the HDEHP color layer powder, the yttrium ions and part of the strontium, cesium, cerium, promethium and other ions in the strontium yttrium solution are adsorbed by the HDEHP color layer powder, then the residual solution is further discharged from the second container 2 under the action of the peristaltic pump 3, and the HDEHP color layer powder adsorbed with the yttrium ions and part of the strontium, cesium, cerium, promethium and other ions is obtained; the strontium yttrium solution in the first container 1 is replaced by 1.5mol / L nitric acid eluent, under the action of the peristaltic pump 3, the 1.5mol / L nitric acid eluent in the first container enters the second container 2 and elutes the HDEHP color layer powder, so that the yttrium ions and part of the strontium, cesium, cerium, promethium and other ions adsorbed in the HDEHP color layer powder are washed out, so that the HDEHP color layer powder adsorbed with the yttrium ions is obtained; then the 1.5mol / L nitric acid eluent in the first container 1 is replaced by 6mol / L nitric acid eluent, and the yttrium ions in the HDEHP color layer powder are eluted alone, so that a solution containing only yttrium ions is obtained; finally, the yttrium ions in the solution are analyzed by precipitation method, and the yttrium to be separated and purified is obtained.
[0031] The flow rate of the solution in the first container 1 through the color layer powder in each step can be accurately controlled, so that the yttrium can be well separated and purified.
[0032] Of course, in addition to the above embodiments, the semi-automatic solution purification device provided by the present application can also be used for the separation and purification of specific substances in other specific solutions, only the type of solution to be separated and the type of color layer powder, eluent, eluent and the like need to be replaced, and the above optional embodiments are only used to illustrate the technical scheme of the present application but not limited.
[0033] In one preferred embodiment, an electric control system can be installed on the peristaltic pump 3, and a control panel is arranged in the electric control system, so that the operator can adjust various output parameters of the peristaltic pump 3 by operating the control panel, so as to more conveniently control the peristaltic pump 3 and more conveniently carry out the whole solution separation process.
[0034] In one preferred embodiment, the second container 2 is provided with several, each of which is loaded with a color layer powder, each of which is in communication with the first container 1, and each of which is provided with a hose 4 at the outlet end, and the plurality of hoses 4 are connected in parallel on the peristaltic pump 3. When the peristaltic pump 3 is working, the plurality of hoses 4 connected in parallel inside it are synchronously affected, so that the plurality of hoses 4 simultaneously generate pressure changes, and the plurality of second containers 2 also simultaneously generate pressure changes, so that the solution to be separated or the eluent can simultaneously enter the plurality of second containers 2, so that the device can realize the simultaneous separation and purification of a plurality of samples.
[0035] Among them, the amount of color layer powder in each second container 2 can be equal, and the output of the peristaltic pump 3 to the plurality of hoses 4 connected in parallel thereon is also completely the same, so that the plurality of hoses 4 and the second containers 2 generate equal pressure changes, so that the solution to be separated (or eluent) can enter each second container 2 equally, and the amount of color layer powder preloaded in each second container 2 is also the same, so that the color layer powder in each second container 2 has the same adsorption effect on the solution to be separated entering it, and the eluent entering each second container 2 has the same elution effect on the color layer powder, thereby obtaining the same color layer powder in the plurality of second containers 2. The adsorbed material to be separated. It has better quality control effect and achieves the purpose of more convenient and rapid separation and purification, and can batch produce the same separation purity and quality of the material to be separated.
[0036] The amount of color layer powder in each second container 2 can also be different, and the peristaltic pump 3 can be adjusted according to the actual situation to make different outputs to the parallel hoses 4, respectively, to control the flow rate of the solution in each separation pipeline, respectively, so that different separation and purification operations can be performed in each separation pipeline formed by the first container 1, the second container 2 and the hose 4.
[0037] In one preferred embodiment, the first container 1 can also be provided with a plurality of, and correspond to a plurality of second containers 2, so that each corresponding first container 1, second container 2 and hose 5 form a separation pipeline, and a plurality of separation pipelines are connected in parallel on the peristaltic pump, the connection relationship between the first container 1 and the second container 2 in each separation pipeline is completely the same, which further ensures that the amount of solution to be separated entering the second container 2 of the hose 4 in each separation pipeline is completely the same after being affected by the peristaltic pump 3, which further improves the quality control effect and achieves the purpose of more convenient and rapid separation and purification.
[0038] Figure 3 It is a whole schematic diagram of the semi-automatic solution purification device provided by another embodiment of the utility model.
[0039] As Figure 3As shown, in one of the preferred embodiments, a third container 6 is further included, and the third container 6 contains a washing liquid or an elution liquid, and only the solution to be separated is contained in the first container 1. After the solution to be separated is introduced into the second container 2, the third container 6 is sealingly connected to the inlet end of the second container 2, so that the washing liquid in the third container 6 is introduced into the second container 2 to wash the color layer powder, and the elution liquid in the third container 6 is used to elute the substance to be separated in the color layer powder.
[0040] Further, an inlet conduit can be sealingly connected to the inlet end of the second container 2, and the inlet conduit is connected to the first container 1 and the third container 6 through a three-way valve 7. By changing the valve state of the three-way valve 7, the inlet conduit is connected to the first container 1 or the third container 6, so that the solution to be separated can be introduced into the second container 2 through the three-way valve 7 and the inlet conduit, and then the washing liquid or the elution liquid can be introduced into the second container 2 through the three-way valve 7 and the inlet conduit.
[0041] In addition, in each group of separation pipelines, two or more third containers 6 can be provided, and each third container 6 contains a solution required in the separation and purification process of the solution to be separated, such as a washing liquid, an elution liquid, etc. Each third container 6 is connected to the inlet conduit through a valve, so that the solution in each third container 6 can be introduced into the second container 2 in the separation and purification process, thereby improving the convenience of the separation and purification operation.
[0042] As shown, Figure 2 As shown, in one of the preferred embodiments, a sealing plug 21 is provided at the inlet end and the outlet end of the second container 2, and the outlet end of the first container 1 and the inlet end of the hose 4 pass through the sealing plug 21 to enter the second container 2, and the sealing plug 21 can sealingly connect the end of the second container 2 to the first container 1 and the hose 4. Further, the outlet end of the first container 1 and the inlet end of the hose 4 can be provided in a needle tube shape, the needle tube is a hollow structure, the tip of the needle tube can be inserted into the sealing plug 21, and the hollow structure of the needle tube can connect the second container 2 to the first container 1 and the hose 4; when the needle tube in the sealing plug 21 is pulled out, the sealing plug 21 still has good sealing effect, which can facilitate the replacement of the solution to be separated and the washing liquid in the first container 1, and ensure that no impurities enter the second container 2 to affect the separation effect during the whole process.
[0043] In one preferred embodiment, the inlet end and the outlet end of the second container 2 are provided with sealing end caps, and end cap valves are arranged on the sealing end caps. The first container 1 and the hose 4 are connected to the sealing end caps. When the end cap valves are opened, the second container 2 is in communication with the first container 1 and the hose 4. When the end cap valves are closed, the second container 2 is isolated from the outside.
[0044] By arranging the sealing plugs or the sealing end caps at the ports of the second container 2, the second container 2 can be connected to the first container 1 and the hose 4 in a sealed manner, and the effectiveness of the peristaltic pump 3 on the pressure change in the separation pipeline is ensured. In addition, the second containers 2 can be pre-filled with the chromatographic powder, and then sealed by the sealing plugs or the sealing end caps to isolate the second containers 2 from the outside, so that the second containers 2 can be used as storage bottles for the chromatographic powder. When the chromatographic powder is needed, the second containers 2 can be directly connected to the first container 1 and the hose 4 through the sealing plugs or the sealing end caps. By sealing the chromatographic powder in the second containers 2, the deterioration of the chromatographic powder can be prevented, and the storage and transportation of the chromatographic powder are facilitated. Moreover, the chromatographic powder is pre-stored in the second containers 2, and there is no need to fill the chromatographic powder on site, so that the separation and purification operation can be facilitated.
[0045] In addition, detachable sealing caps can also be arranged at the inlet and outlet ports of the second container 2. When the separation and purification operation is needed, the sealing caps are removed, and then the second container 2 is connected to the first container 1 and the hose 4 in a sealed manner.
[0046] As shown in FIG. 2, Figure 2 In one preferred embodiment, a second fixed sheet 23 having the same material and shape as the first fixed sheet 22 is further arranged. The second fixed sheet 23 is detachably arranged in the second container 2 along the axial direction of the second container 2, so that the chromatographic powder is clamped between the two fixed sheets in the second container 2. With the arrangement of the two fixed sheets, when the chromatographic powder is pre-filled in the second container 2, the first fixed sheet 22 is first arranged, and then a certain amount of the chromatographic powder is placed to one side of the first fixed sheet 22. Then, the second fixed sheet 23 is arranged in the second container 2, so that the chromatographic powder is fixed relative to the second container 2, and the chromatographic powder is prevented from being shaken greatly during long-term storage and transportation in the second container 2.
[0047] Moreover, the solution to be separated or the eluent entering the second container 2 from above the second fixed sheet 23 flows to the chromatographic powder through the second fixed sheet 23. The second fixed sheet 23 has a porous structure, and when the liquid passes through the porous structure of the second fixed sheet 23, the liquid can diffuse radially in the porous structure, so that the uniformity and completeness of the contact between the liquid and the chromatographic powder are improved, and the separation and purification effect is improved.
[0048] In addition, since the partial color layer powder has a small density, the liquid flowing through the color layer powder can scatter the color layer powder, affecting the separation and purification effect. By arranging two fixing pieces, the color layer powder is fixed in the second container 2, ensuring that the color layer powder is in a cylindrical shape as a whole and has strong stability, thereby ensuring the separation and purification effect.
[0049] In the preferred embodiment, the outer periphery of the two fixing pieces is slightly smaller than the inner wall profile of the second container 2, and an elastic edge ring, such as a rubber sealing ring, is arranged on the outer periphery of the two fixing pieces. The outer shape of the elastic edge ring is slightly larger than the inner wall profile of the second container 2. The fixing pieces are inserted into the second container 2, and the elastic edge ring on the outer periphery of the fixing pieces is attached to the inner wall of the second container 2, so that the fixing pieces are installed in the second container 2. Of course, the fixing pieces can be connected to the second container 2 in other ways, as long as the fixing effect on the color layer powder is achieved.
[0050] As shown in Figure 1 In one preferred embodiment, the first container 1 includes a loading part 11 and a conduit part 12. The loading part 11 contains the solution to be separated or the eluent. One end of the conduit part 12 is connected to the bottom of the loading part 11, and the other end is connected to the upper end of the second container 2. A valve 121 is arranged on the conduit part. By changing the opening and closing state of the valve 121, the first container 1 and the second container 2 can be connected or disconnected.
[0051] As shown in Figure 1 In one preferred embodiment, a collection bottle 5 is arranged at the outlet end of the hose 4. By operating the peristaltic pump, the pressure state in the separation pipeline is changed, and the liquid can be pumped into the collection bottle 5 through the hose 4, thereby completing the collection of the solution after separation and purification. For example, during the separation process, the solution to be separated is introduced into the separation pipeline, and the separated substance in the solution to be separated is adsorbed by the color layer powder. The waste liquid is discharged from the hose into the collection bottle 5. During the elution process, the collection bottle 5 is replaced, and then the eluent is introduced into the separation pipeline. The eluent absorbs the separated substance in the color layer powder, and then enters the collection bottle 5 through the hose, thereby completing the collection of the high-purity separated substance solution.
[0052] In addition, a mounting rack can be arranged. The first container 1, the second container 2, the peristaltic pump 3, and the collection bottle 5 can be sequentially connected and placed on the mounting rack. The first container 1 is located above the second container 2, and the second container 2 is located above the peristaltic pump 3, which facilitates the installation and connection of the entire separation pipeline and the separation and purification process.
[0053] Finally, it should be noted that the above examples are intended to illustrate the technical solutions of the present application and are not limiting. Although the present application has been described in detail with reference to the examples, those skilled in the art should understand that modifications or equivalent replacements of the technical solutions of the present application do not deviate from the spirit and scope of the present application, and they should be covered in the scope of the claims of the present application.
Claims
1. A semi-automated solution purification device, characterized in that, The application relates to a chromatographic column, which comprises: a first container containing a solution to be separated or a leaching liquid or an eluent; a second container, the inlet end of which is sealingly connected with the outlet end of the first container, and the first fixed sheet is arranged in the second container, and the color layer powder is arranged on the side of the first fixed sheet close to the second container, the first fixed sheet is semi-permeable, and can allow the solution to be separated or the leaching liquid or the eluent to pass through and block the color layer powder from passing through; a peristaltic pump, the outlet end of the second container is sealingly connected with the peristaltic pump through a hose, and the pressure state in the hose and the second container is changed through the peristaltic pump, so that the solution to be separated or the leaching liquid enters the second container and flows through the color layer powder, so that the separated substance in the solution to be separated is attached to the color layer powder, or the leaching liquid is used to leach the color layer powder, or the eluent is used to elute the separated substance in the color layer powder.
2. The semi-automated solution purification device of claim 1, wherein, The second container is provided with a plurality of second containers, each of which is loaded with color layer powder, each of which is in communication with the first container, and the outlet end of each of which is provided with the hose, and the plurality of hoses are connected in parallel on the peristaltic pump.
3. The semi-automated solution purification device of claim 2, wherein, The first container is provided with a plurality of first containers corresponding to the number of second containers, and each of the second containers is in communication with the corresponding first container.
4. The semi-automated solution purification device of claim 1, wherein, The application further comprises a third container, the first container contains the solution to be separated, and the third container contains the leaching liquid or the eluent; The second container is sealingly connected with the first container to perform the adsorption separation operation on the solution to be separated, or the second container is sealingly connected with the third container to perform the leaching or elution operation on the color layer powder.
5. The semi-automated solution purification device of claim 4, wherein, The second container is sealingly connected with an inlet pipe, the inlet pipe is in communication with the first container and the third container through a three-way valve, and the communication state of the inlet pipe with the first container and the third container is controlled by changing the valve state of the three-way valve.
6. The semi-automated solution purification device of claim 1, wherein, Sealing rubber plugs are arranged at the inlet end and the outlet end of the second container, and the outlet end of the first container and the inlet end of the hose pass through the sealing rubber plugs to enter the second container, so as to realize the sealing connection with the second container.
7. The semi-automated solution purification device of claim 1, wherein, Sealing end covers are arranged at the inlet end and the outlet end of the second container, end cover valves are arranged on the sealing end covers, and the first container and the hose are connected to the sealing end covers, so that the second container is in the communication state or the closed state by opening and closing the end cover valves.
8. The semi-automated solution purification device of claim 6 or 7, wherein, The application further comprises a second fixed sheet, the second fixed sheet is completely same in material and shape with the first fixed sheet, the second fixed sheet and the first fixed sheet are detachably installed in the second container along the axial direction of the second container, and the color layer powder is stored between the second fixed sheet and the first fixed sheet.
9. The semi-automated solution purification device of claim 1, wherein, The first container comprises a loading part in which the solution to be separated or the eluent is contained, and a conduit part, one end of which is communicated with the bottom end of the loading part, and the other end of which is connected to the upper end of the second container, and a valve is arranged on the conduit part.
10. The semi-automated solution purification device of claim 1, wherein, A collecting bottle is arranged at the outlet end of the hose.