Extraction device for stem cell exosome preparation

Abstract

The utility model belongs to the technical field of stem cell exosome preparation, and relates to an extraction device for stem cell exosome preparation, which comprises an extractor body and an extractor head in threaded connection. An extraction barrel is rotationally arranged in the extractor body in a limiting manner, a plurality of liquid storage cavities are formed in the extractor body in the circumferential direction of the extraction barrel, and sealing covers are connected to the top ends of the liquid storage cavities in a clamped manner; a piston is arranged in the extraction cylinder in an up-down moving manner and is in sealed connection with the inner wall of the extraction cylinder; a long rod is fixedly connected to the upper end face of the piston, and the top end of the long rod extends to the position above the extraction barrel; the one-way valve is arranged at the extractor head, a vacuum cavity can be formed in the extraction barrel when the extraction barrel rotates and the piston moves upwards, and due to the fact that the cavity is in a vacuum state, mixed liquid in the liquid storage cavity can rapidly flow into the cavity, in a low-pressure state, of the extraction barrel from the normal-pressure end, the speed of the mixed liquid flowing into the extraction barrel is increased, and the extraction efficiency is improved. The preparation and extraction efficiency of the stem cell exosome is further improved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to stem cell exosome preparation technical field relates to a kind of stem cell exosome preparation's extraction device. BACKGROUND

[0002] Exosomes naturally exist in body fluids, such as blood, saliva, urine, cerebrospinal fluid and milk, etc. The study of exosome structure and function is the research that researchers are keen on, and in the past, it is necessary to extract from exosome mixed solution.

[0003] In the Chinese patent CN220597423U, a cell exosome extractor is disclosed, which includes a body and a head. The body and the head are threadedly connected. The head is provided with a lower liquid cavity. A plurality of layers of filter membranes are fixed in the lower liquid cavity. The body is cylindrical. An extraction cylinder is arranged in the body. The lower liquid cavity and the extraction cylinder are coaxial. A plurality of storage units are arranged in the body along the circumference of the extraction cylinder. A lower pressing piston is arranged in the extraction cylinder.

[0004] The above-mentioned existing cell exosome extractor needs to pull the lower pressing piston to the top of the extraction cylinder first, then pull the corresponding plug-in block, so that the exosomes enter the inside of the extraction cylinder through the connecting port. When more exosomes are needed, other plug-in blocks need to be pulled up, which makes the operation process more complicated, and the speed of exosomes entering the extraction cylinder is slow, which reduces the efficiency of stem cell exosome preparation and extraction.

[0005] To solve the above problems, the utility model provides an extraction device for stem cell exosome preparation. UTILITY MODEL CONTENT

[0006] To solve the problems in the background art, the utility model provides an extraction device for stem cell exosome preparation.

[0007] To achieve the above-mentioned purpose, the utility model adopts the following technical scheme: an extraction device for stem cell exosome preparation, including extractor body and extractor head, the extractor body and the extractor head are threadedly connected; an extraction cylinder is rotationally arranged inside the extractor body; a plurality of liquid storage cavities are arranged in the extractor body along the circumference of the extraction cylinder; a sealing cover is connected to the top end of each liquid storage cavity; a piston is movably arranged inside the extraction cylinder; the piston is sealingly connected to the inner wall of the extraction cylinder; a long rod is fixedly connected to the upper end surface of the piston; the top end of the long rod extends above the extraction cylinder; a sliding sleeve is slidingly arranged on the outside of the long rod; the sliding sleeve is connected to the top end of the extraction cylinder through a ball screw pair; the top end of the sliding sleeve is limitingly connected to the long rod; a liquid leakage hole is formed in the bottom end of each liquid storage cavity; a liquid discharge hole is formed in the bottom end of the extraction cylinder corresponding to the position of the liquid leakage hole; and a positioning assembly is arranged at the top end of the extraction cylinder.

[0008] Preferably, the top end of the extraction cylinder is inlaid with a ball nut, and the outer side of the sliding sleeve is provided with external threads, and the external threads on the sliding sleeve are connected with the ball nut to form a ball screw structure.

[0009] Preferably, the top end of the sliding sleeve is externally threaded and is provided with a fastening bolt, and the bottom end of the fastening bolt abuts against the outer side of the long rod.

[0010] Preferably, the top end of the inner wall of the extractor body is provided with an annular sliding groove, and the outer side of the extraction cylinder is fixedly connected with a sliding ring which is rotatably installed in the annular sliding groove.

[0011] Preferably, the upper end face of the extractor body is fixedly connected with clamping blocks on both sides of the liquid storage cavity, and the clamping blocks are provided with insertion grooves on both sides; the two sides of the sealing cover are fixedly connected with clamping blocks, and the clamping blocks on the two sides of the sealing cover are inserted into the insertion grooves between adjacent two clamping blocks; and the upper end face of the sealing cover is provided with pulling grooves.

[0012] Preferably, the positioning assembly comprises a pulling ring, a limiting block, a limiting rod and a connecting seat; the pulling ring is fixedly connected to the top end of the sliding sleeve, the upper end face of the clamping block is fixedly connected with the limiting block on one side close to the pulling ring, the outer side of the top end of the extraction cylinder is fixedly connected with the connecting seat at the position corresponding to the limiting block, the connecting seat is rotatably connected with the limiting rod, and the limiting rod and the connecting seat are fixedly connected with a torsion spring; and the limiting rod abuts against the limiting block.

[0013] Compared with the prior art, the utility model has the following beneficial effects:

[0014] 1、The extraction device for preparing stem cell exosome, by setting the one-way valve at the extractor head, can form a vacuum cavity in the extraction cylinder when the extraction cylinder rotates and the piston moves upwards, the mixed solution in the liquid storage cavity can flow into the cavity in the extraction cylinder in low pressure state from the normal pressure end due to the vacuum state of the cavity, the speed of the mixed solution flowing into the extraction cylinder is improved, and the efficiency of the stem cell exosome preparation and extraction is further improved.

[0015] 2、The extraction device for preparing stem cell exosome, a plurality of liquid storage cavities are arranged on the extractor body, a plurality of liquid leakage holes are arranged on the wall of the liquid storage cavity, and a liquid discharge hole is arranged on the extraction cylinder, the mixed solution in the cavity is introduced into the extraction cylinder by rotating the extraction cylinder to make the liquid discharge hole correspond to the liquid leakage hole, and when more mixed solution is needed, the extraction cylinder is continuously rotated to make the liquid discharge hole correspond to the liquid leakage hole of the next liquid storage cavity, so that the operation is simple and the device is convenient to use. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 is a schematic diagram of the three-dimensional structure of the embodiment 1 of the utility model;

[0017] Figure 2 is a schematic diagram of the three-dimensional structure of the embodiment 1 of the utility model Figure 1 is an enlarged schematic diagram of A in the utility model

[0018] Figure 3 is the extractor body and the extractor head connection schematic diagram of the utility model;

[0019] Figure 4 is the local section view schematic diagram of the utility model embodiment 1;

[0020] Figure 5 is the extractor inside schematic diagram of the utility model embodiment 1;

[0021] Figure 6 is the liquid discharge hole position schematic diagram of the utility model embodiment 1.

[0022] In the figure: 1, extractor body;2, clamping block;3, sealing cover;4, long rod;5, pull ring;6, limit block;7, extractor head;8, fastening bolt;9, extraction cylinder;10, sliding sleeve;11, piston;16, limit rod;17, connecting seat;18, liquid storage cavity;19, liquid leakage hole;20, annular sliding groove;21, pulling groove;22, sliding ring;23, liquid discharge hole. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the utility model will be described clearly and completely below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the utility model.

[0024] As Figures 1-6 shown, the technical scheme adopted by the utility model is as follows: an extraction device for stem cell exosome preparation, comprising an extractor body 1 and an extractor head 7, the extractor body 1 and the extractor head 7 are threadedly connected. A lower liquid cavity is formed in the extractor head 7, and a filter membrane is fixedly arranged in the lower liquid cavity.

[0025] The extractor body 1 is internally rotationally limited and provided with an extraction cylinder 9. The lower liquid cavity and the extraction cylinder 9 are coaxially arranged.

[0026] Specifically, the inner wall top end of the extractor body 1 is provided with an annular sliding groove 20, the outer side of the extraction cylinder 9 is fixedly connected with a sliding ring 22, and the sliding ring 22 is rotationally installed inside the annular sliding groove 20.

[0027] The extractor body 1 is internally provided with a plurality of liquid storage cavities 18 along the circumference of the extraction cylinder 9, and the top end of each liquid storage cavity 18 is clamped and connected with a sealing cover 3.

[0028] Specifically, the upper end face of the extractor body 1 corresponds to the two sides of the liquid storage cavity 18 and is fixedly connected with clamping blocks 2, and the two sides of the clamping block 2 are provided with insertion grooves. The two sides of the sealing cover 3 are fixedly connected with clamping blocks, and the clamping blocks on the two sides of the sealing cover 3 are inserted into the insertion grooves between the adjacent two clamping blocks 2. The upper end face of the sealing cover 3 is provided with a pulling groove 21.

[0029] The inside of the extraction cylinder 9 is movably provided with a piston 11, and the piston 11 is sealingly connected with the inner wall of the extraction cylinder 9. The upper end face of the piston 11 is fixedly connected with a long rod 4, and the top end of the long rod 4 extends above the extraction cylinder 9. The outer side of the long rod 4 is slidingly sleeved with a sliding sleeve 10.

[0030] The sliding sleeve 10 is connected with the top end of the extraction cylinder 9 through a ball screw pair.

[0031] Specifically, the inside of the top end of the extraction cylinder 9 is inlaid with a ball nut, and the outer side of the sliding sleeve 10 is provided with external threads, and the external threads on the sliding sleeve 10 and the ball nut are connected through balls to form a ball screw structure.

[0032] The top end of the sliding sleeve 10 is limitingly connected with the long rod 4.

[0033] Specifically, the top end of the sliding sleeve 10 is externally threaded and installed with a fastening bolt 8, and the bottom end of the fastening bolt 8 abuts against the outer side of the long rod 4.

[0034] The inside bottom end of the liquid storage cavity 18 is provided with a liquid leakage hole 19, and the bottom end of the extraction cylinder 9 is provided with a liquid discharge hole 23 corresponding to the position of the liquid leakage hole 19.

[0035] The top end of the extraction cylinder 9 is provided with a positioning assembly.

[0036] The positioning assembly comprises a pull ring 5, a limiting block 6, a limiting rod 16 and a connecting seat 17. The pull ring 5 is fixedly connected to the top end of the sliding sleeve 10, the upper end face of the clamping block 2 is fixedly connected with the limiting block 6 near one side of the pull ring 5, the outer top end of the extraction cylinder 9 is fixedly installed with the connecting seat 17 corresponding to the position of the limiting block 6, the connecting seat 17 is rotatably installed with the limiting rod 16, and the limiting rod 16 and the connecting seat 17 are fixedly connected with a torsion spring. The limiting rod 16 abuts against the limiting block 6.

[0037] Working principle:

[0038] In use, the sealing cover 3 is opened, mixed liquid is injected into each liquid storage cavity 18, and then the sealing cover 3 is clamped with the clamping block 2 to seal the liquid storage cavity 18.

[0039] Pulling up the pull ring 5, the pull ring 5 moves upwards to drive the sliding sleeve 10 to move upwards, because the fastening bolt 8 limits the long rod 4, so when the sliding sleeve 10 moves upwards, the piston 11 in the extraction cylinder 9 will be driven to move upwards, because the piston 11 is sealingly connected with the extraction cylinder 9, and the extractor head 7 is provided with a one-way valve, so that the piston 11 moves upwards to form a cavity in the extraction cylinder 9 in a vacuum state.

[0040] At the same time, the upward movement of the sliding sleeve 10 can also drive the extraction cylinder 9 to rotate in the annular sliding groove 20 through the ball screw pair, and when the limiting rod 16 is in contact with the limiting block 6, the liquid outlet hole 23 on the extraction cylinder 9 is just opposite to the liquid leakage hole 19 on the liquid storage cavity 18, because the cavity is in a vacuum state, the mixed liquid in the liquid storage cavity 18 under normal pressure will flow into the cavity in the extraction cylinder 9 under low pressure, which improves the speed of the mixed liquid flowing into the extraction cylinder 9, and further improves the efficiency of the preparation and extraction of stem cell exosomes.

[0041] When the amount of mixed liquid in the two chambers is needed, continue to pull the pull ring 5, at this time the limiting rod 16 rotates around the connecting seat 17, and then passes through the limiting block 6, the sleeve continues to move upwards to drive the extraction cylinder 9 to continue to rotate, so that the liquid outlet hole 23 on the extraction cylinder 9 corresponds to the liquid leakage hole 19 of the next chamber, and then more mixed liquid is released into the inside of the extraction cylinder 9.

[0042] When the amount of mixed liquid in the extraction cylinder 9 reaches the expected value, the fastening bolt 8 is screwed to release the limiting of the long rod 4 by the fastening bolt 8, the long rod 4 is pressed down to drive the piston 11 to move downwards, so that the mixed liquid in the extraction cylinder 9 flows out from the extractor head 7, and then the preparation and extraction of stem cell exosomes is completed.

[0043] Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement to part of the technical features, and any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the utility model shall be included in the protection scope of the utility model.

Claims

1. A stem cell exosome preparation extraction device, comprising an extractor body (1) and an extractor head (7), the extractor body (1) and the extractor head (7) are threadedly connected; characterized in that, The inside of the extractor body (1) is rotationally limited and provided with an extraction cylinder (9), a plurality of liquid storage cavities (18) are arranged along the circumference of the extraction cylinder (9) in the extractor body (1), the top end of each liquid storage cavity (18) is clamped and connected with a sealing cover (3); the inside of the extraction cylinder (9) is movably arranged up and down with a piston (11), the piston (11) is sealingly connected with the inner wall of the extraction cylinder (9); the upper end surface of the piston (11) is fixedly connected with a long rod (4), the top end of the long rod (4) extends above the extraction cylinder (9); the outer side of the long rod (4) is slidingly sleeved with a sliding sleeve (10), the top end of the sliding sleeve (10) is connected with the top end of the extraction cylinder (9) through a ball screw pair; the top end of the sliding sleeve (10) is limitedly connected with the long rod (4), the inside bottom end of each liquid storage cavity (18) is provided with a liquid leakage hole (19), the bottom end of the extraction cylinder (9) is provided with a liquid discharge hole (23) corresponding to the position of the liquid leakage hole (19), and the top end of the extraction cylinder (9) is provided with a positioning assembly.

2. The extraction device for stem cell exosome preparation according to claim 1, characterized in that: The inside of the top end of the extraction cylinder (9) is inlaid with a ball nut, and the outer side of the sliding sleeve (10) is provided with an external thread, and the external thread on the sliding sleeve (10) is connected with the ball nut to form a ball screw structure.

3. The extraction device for stem cell exosome preparation according to claim 1, characterized in that: The top end of the sliding sleeve (10) is externally screwed with a fastening bolt (8), and the bottom end of the fastening bolt (8) abuts against the outer side of the long rod (4).

4. The extraction device for stem cell exosome preparation according to claim 1, characterized in that: The top end of the extractor body (1) is provided with an annular sliding groove (20), and the outer side of the extraction cylinder (9) is fixedly connected with a sliding ring (22), and the sliding ring (22) is rotationally installed in the annular sliding groove (20).

5. The extraction device for stem cell exosome preparation according to claim 1, characterized in that: The upper end surface of the extractor body (1) is fixedly connected with clamping blocks (2) corresponding to the two sides of the liquid storage cavities (18), and the two sides of each clamping block (2) are provided with a plug-in groove; the two sides of the sealing cover (3) are fixedly connected with clamping blocks, and the clamping blocks on the two sides of the sealing cover (3) are plugged into the plug-in grooves between the adjacent two clamping blocks (2); the upper end surface of the sealing cover (3) is provided with a pulling groove (21).

6. The extraction device for stem cell exosome preparation according to claim 1, characterized in that: The positioning assembly comprises a pull ring (5), a limiting block (6), a limiting rod (16) and a connecting seat (17); the pull ring (5) is fixedly connected to the top end of the sliding sleeve (10), the upper end surface of each clamping block (2) is fixedly connected with a limiting block (6) on the side close to the pull ring (5), the outer top end of the extraction cylinder (9) is fixedly connected with a connecting seat (17) corresponding to the position of the limiting block (6), the connecting seat (17) is rotationally installed with a limiting rod (16), and the limiting rod (16) and the connecting seat (17) are fixedly connected with a torsion spring; the limiting rod (16) abuts against the limiting block (6).

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