Circulating device for d-ribose fermentation
By setting up a premixed fermentation chamber and a pump circulation pipe on the fermenter, combined with a continuous feeding unit and a feeding screw, the problem of fermentation balance being easily disrupted during microbial fermentation was solved, thereby increasing the concentration of microorganisms and stabilizing the yield, while reducing contamination by other microorganisms.
Patent Information
- Application Number
- CN202423073495.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-12
- Publication Date
- 2025-12-16
- Estimated Expiration
- 2034-12-12
AI Technical Summary
Existing microbial fermentation methods for producing d-ribose have poor fermentation continuity, requiring intermittent replenishment of fresh culture medium, which easily disrupts the fermentation balance, leading to yield fluctuations and microbial imbalance, and is also susceptible to contamination by other microorganisms.
A premixed fermentation chamber is set up on the fermenter. The microbial solution is filtered into the premixed fermentation chamber through a pump circulation pipe. The culture medium injection pipe is used for preliminary fermentation. The preliminary fermentation mixture is slowly fed into the fermenter using a continuous feeding unit and a feeding screw to extend the residence time, reduce environmental disturbance, increase the concentration of microorganisms, and create a stable environment.
It increases the concentration of microorganisms, reduces microbial imbalance and contamination, improves the stability of the fermentation process and the continuity of yield, and ensures product quality.
Smart Images
Figure CN223674611U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to fermentation equipment technical field. BACKGROUND
[0002] D-ribose is an important component of genetic material - ribonucleic acid (RNA) in organisms, and plays a pivotal role in nucleoside substances, protein and fat metabolism, and has important physiological functions and broad application prospects. As a natural ingredient in organisms, d-ribose is present in all cells and is closely related to the formation of adenosine and the regeneration of adenosine triphosphate (ATP), and is one of the most basic energy sources for life metabolism. It plays a key role in heart and skeletal muscle metabolism and can promote the recovery of ischemic and hypoxic tissues.
[0003] Nucleic acid drugs are an important means for treating viruses, tumors and AIDS in humans today, and d-ribose is an important intermediate for many nucleic acid drugs, which can be used in the production of many drugs such as triazol nucleoside, adenosine, thymidine, cytidine, fluoroadenine nucleoside, 2-methyl adenosine, veltosin, pyrazole toxin, adenosine and many others.
[0004] Due to the pollution caused by chemical synthesis, in recent years, countries have successively researched microbial fermentation method to produce d-ribose, and strive to industrialize production. The microbial fermentation method for industrial production of d-ribose includes fed-batch fermentation method: this fermentation method intermittently supplements fresh medium during batch fermentation to maintain low substrate concentration in the fermentation system. A general d-ribose fermentation tank includes a tank body, a stirring system, a ventilation and exhaust device, a temperature and pressure control system, and a feeding and discharging system.
[0005] However, the above-mentioned existing fermentation method has the following disadvantages: the fermentation process has poor continuity, and fresh medium needs to be supplemented at intervals, which can greatly affect the microorganisms that have formed a stable ecology in the fermentation tank, and can easily destroy the fermentation balance in the tank. The microorganisms in the tank need to be re-propagated, and during this period, they are easily contaminated by miscellaneous bacteria, causing large fluctuations in yield, and affecting product quality. UTILITY MODEL CONTENT
[0006] The utility model aims at: in order to solve the above technical problem, the utility model provides a circulating device for d-ribose fermentation.
[0007] The utility model realizes the above-mentioned purpose by adopting the following technical solutions:
[0008] The utility model provides a kind of d-ribose fermentation circulating device, including fermenter, stirring mechanism is equipped in the fermenter, temperature control pressure control mechanism, discharge port, premixing fermentation bin is equipped on the fermenter, medium injection pipe and strain circulating inoculation mechanism are equipped in the premixing fermentation bin, the strain circulating inoculation mechanism includes the pumping circulation pipe being equipped in the fermenter, filter screen is equipped at the joint of the pumping circulation pipe with the fermenter, the upper end of the pumping circulation pipe is inserted into the premixing fermentation bin and is equipped with inoculation mechanism in end, continuous feeding unit is equipped between the premixing fermentation bin with the fermenter, and the continuous feeding unit includes motor-driven feeding screw being equipped at the bottom end of the premixing fermentation bin, and the output end of the feeding screw is connected into the fermenter by pipeline.
[0009] Through the above scheme, by setting premixing fermentation bin on ordinary fermenter, the microbial solution in fermenter is filtered and pumped to premixing fermentation bin through filter screen by pumping circulation pipe, medium injection pipe is matched, and the microbial solution is injected into the medium in premixing fermentation bin by inoculation mechanism, preliminary fermentation is continuously carried out, microbial concentration is proliferated, and the mixture completing preliminary fermentation is connected into fermenter by pipeline through continuous feeding unit, i. e. feeding screw, the whole process is continuously pumped into premixing fermentation bin and continuously fed into medium, and preliminary mixed fermentation is completed in premixing fermentation bin, then it is continuously and slowly connected into fermenter, the whole process is preliminarily fermented by premixing fermentation bin, the microbial concentration is improved, the microbial environment is created, the disturbance to the internal environment of fermenter is reduced by continuous and slow feeding, and the imbalance of flora is reduced.
[0010] Further, the continuous feeding unit further comprises a feeding pipe provided at the output end of the feeding screw, the feeding pipe is arranged around the outer periphery of the fermenter and has a terminal end connected into the fermenter.
[0011] Through the above scheme, the feeding pipe is arranged around the outer periphery of the fermenter and has a terminal end connected into the fermenter, the residence time of the preliminary mixed mixture in the premixing fermentation bin and the feeding pipe is prolonged by lengthening the length of the feeding pipe, the preliminary fermentation time is prolonged, and the number of microorganisms in the mixture is increased.
[0012] Further, the feeding pipe is provided with a high-pressure flushing pipeline, the high-pressure flushing pipeline is provided with an electromagnetic valve, the high-pressure flushing pipeline is connected with an external high-pressure water source, and the high-pressure flushing pipeline is provided with at least two and distributed on the feeding pipe.
[0013] Through the above scheme, the high-pressure flushing pipeline is provided to facilitate the injection of high-pressure water flow into the feeding pipe from multiple directions, and the inside of the feeding pipe is conveniently and quickly cleaned.
[0014] Further, the inoculation mechanism comprises an inoculation disc arranged at the upper end of the pumping circulation pipe, wherein the inoculation disc is provided with inoculation needles arranged towards the bottom end of the premixing fermentation bin, and the medium injection pipe is arranged opposite to the inoculation needles.
[0015] Through the above scheme, the inoculation disc is arranged to facilitate the inoculation needles, and the pumping circulation pipe continuously feeds the microbial solution into the inoculation disc and finally discharges the microbial solution from the inoculation needles, so that the microbial colony is inoculated and mixed uniformly.
[0016] Further, the premixing fermentation bin is internally provided with a circulating pump, wherein the inlet end of the circulating pump is arranged to extend into the bottom end of the premixing fermentation bin, and the outlet end of the circulating pump is arranged to extend into the upper end of the premixing fermentation bin.
[0017] Through the above scheme, the circulating pump is arranged to continuously discharge the materials in the premixing fermentation bin from the bottom end to the upper end, so as to realize the circulation and stirring of the materials.
[0018] Further, the top of the premixing fermentation bin is provided with an electric heating plate, the electric heating plate is electrically connected with a power supply, and the premixing fermentation bin is internally provided with a thermometer.
[0019] Through the above scheme, the electric heating plate is arranged to cooperate with the thermometer, so as to adjust and control the temperature in the premixing fermentation bin and maintain the suitable fermentation temperature.
[0020] Further, the outer periphery of the feeding spiral channel is provided with an inspection opening.
[0021] Through the above scheme, the inspection opening is arranged to facilitate the inspection of the feeding spiral channel.
[0022] Further, the feeding pipe and the outer periphery of the premixing fermentation bin are both covered with a heat preservation layer.
[0023] Through the above scheme, the heat preservation layer is arranged to perform heat preservation.
[0024] The beneficial effects of the utility model are as follows:
[0025] 1. The utility model discloses a simple structure, it is through setting up premixing fermentation bin on the ordinary fermentation tank, through the pump circulating pipe, the microbial solution in the fermentation tank is filtered to the premixing fermentation bin through the filter screen, and the medium injection pipe is injected, and the microbial solution is injected into the medium in the premixing fermentation bin through inoculation mechanism, and preliminary fermentation is carried out constantly, and the microbial concentration is proliferated, and the mixture that completes preliminary fermentation is passed through the pipeline and is passed into the fermentation tank through the continuous feeding unit, the whole process is constantly drawn into the premixing fermentation bin and is constantly put into the medium, and preliminary mixed fermentation is completed in the premixing fermentation bin, then slowly into the fermentation tank constantly, the whole process is through the premixing fermentation tank preliminary fermentation, improves the microbial concentration, creates the microbial environment, through the continuous slow feeding, reduces the disturbance to the internal environment of the fermentation tank, reduces the imbalance of flora.
[0026] 2. The inoculation disc is convenient for bearing inoculation needle, and the microbial solution is constantly passed into the inoculation disc and is finally discharged from the inoculation needle, at this time, the medium in the medium injection pipe is constantly mixed, and the microbial community inoculation is completed, and the mixture is uniform. BRIEF DESCRIPTION OF DRAWINGS
[0027] Figure 1 It is the structure diagram of the utility model;
[0028] Drawing mark: 11, fermentation tank;12, stirring mechanism;13, discharge port;14, premixing fermentation bin;15, medium injection pipe;16, pump circulating pipe;17, filter screen;18, feeding screw;19, feeding pipe;20, high pressure flushing pipeline;21, electromagnetic valve;22, inoculation disc;23, inoculation needle;24, circulating pump;25, electric heating plate;26, thermometer;27, inspection port. DETAILED DESCRIPTION
[0029] In order to make the purpose, technical scheme and advantage of the utility model embodiment clearer, below, the technical scheme in the utility model embodiment will be clearly and completely described with the drawings in the utility model embodiment, obviously, the described embodiment is a part of the embodiment of the utility model, rather than all the embodiment. The components of the utility model embodiment described and shown in the drawing here can be arranged and designed in various different configurations.
[0030] Therefore, the following detailed description of the embodiment of the utility model provided in the drawing is not intended to limit the scope of the claimed utility model, but only represents selected embodiments of the utility model. Based on the embodiments in the utility model, all other embodiments obtained by ordinary skilled persons in the art without creative labor belong to the scope of protection of the utility model.
[0031] Embodiment 1
[0032] As Figure 1 shown, the embodiment provides a circulating device for d-ribose fermentation, which comprises a fermentation tank 11, a stirring mechanism 12, a temperature and pressure control mechanism, a discharge port 13 arranged in the fermentation tank 11, and a premixing fermentation bin 14 arranged on the fermentation tank 11. The stirring mechanism 12 and the temperature and pressure control mechanism are configured for the common fermentation tank 11, and thus are not described in detail. The premixing fermentation bin 14 is provided with a culture medium injection pipe 15 and a strain circulating inoculation mechanism. The strain circulating inoculation mechanism comprises a pumping circulation pipe 16 arranged in the fermentation tank 11. A filter screen 17 is arranged at the connection between the pumping circulation pipe 16 and the fermentation tank 11. The upper end of the pumping circulation pipe 16 extends into the premixing fermentation bin 14 and is provided with an inoculation mechanism at the end. A continuous feeding unit is arranged between the premixing fermentation bin 14 and the fermentation tank 11. The continuous feeding unit comprises a motor-driven feeding screw 18 arranged at the bottom end of the premixing fermentation bin 14. The output end of the feeding screw 18 is connected to the fermentation tank 11 through a pipeline. The continuous feeding unit further comprises a feeding pipe 19 arranged at the output end of the feeding screw 18. The feeding pipe 19 is arranged around the outer periphery of the fermentation tank 11 and has an end connected to the fermentation tank 11.
[0033] Therefore, by arranging the premixing fermentation bin 14 on the common fermentation tank 11, the microbial solution in the fermentation tank 11 is filtered by the filter screen 17 and pumped to the premixing fermentation bin 14 through the pumping circulation pipe 16. The microbial solution is injected into the culture medium in the premixing fermentation bin 14 through the inoculation mechanism in cooperation with the culture medium injection pipe 15, so as to continuously perform preliminary fermentation and proliferate the microbial concentration. The mixture after preliminary fermentation is connected to the fermentation tank 11 through the pipeline by the continuous feeding unit, i.e., the feeding screw 18. The feeding pipe 19 is arranged around the outer periphery of the fermentation tank 11 and has an end connected to the fermentation tank 11. By prolonging the length of the feeding pipe 19, the residence time of the preliminary mixed mixture in the premixing fermentation bin 14 and the feeding pipe 19 is prolonged, so as to prolong the preliminary fermentation time and improve the number of microbial colonies in the mixture. The mature microbial solution is continuously pumped into the premixing fermentation bin 14 and continuously fed into the culture medium. The preliminary mixed fermentation in the premixing fermentation bin 14 is completed, and then the mixture is continuously and slowly connected to the fermentation tank 11. The whole process is preliminarily fermented by the premixing fermentation bin 11, the microbial concentration is improved, the microbial environment is created, the disturbance to the internal environment of the fermentation tank 11 is reduced by continuously and slowly feeding, and the imbalance of the microbial population is reduced.
[0034] Referring to Figure 1, in order to improve the operation efficiency of the device, especially improve the inoculation efficiency of microbial community, the inoculation mechanism includes the inoculation disc 22 arranged at the upper end of the pumping circulation pipe 16, the inoculation disc 22 is arranged with the inoculation needle 23, the inoculation needle 23 is arranged towards the bottom end of the premixing fermentation bin 14, the medium injection pipe 15 is arranged opposite to the inoculation needle 23, the circulation pump 24 is arranged in the premixing fermentation bin 14, the inlet end of the circulation pump 24 extends into the bottom end of the premixing fermentation bin 14, and the outlet end of the circulation pump 24 extends into the upper end of the premixing fermentation bin 14. The inoculation disc 22 is arranged to facilitate the inoculation needle 23, and the pumping circulation pipe 16 continuously passes the microbial solution into the inoculation disc 22 and finally discharges from the inoculation needle 23, at this time, the inoculation disc 22 is mixed with the medium continuously discharged from the medium injection pipe 15, the microbial community is inoculated, the mixture is uniform, and the circulation pump 24 is arranged to continuously discharge the material in the premixing fermentation bin 14 from the bottom end to the upper end, so that the material is circulated and stirred.
[0035] Referring to Figure 1 , the top of the premixing fermentation bin 14 is provided with an electric heating plate (a conventional element, not shown in the figure), and the electric heating plate is electrically connected with a power supply; a thermometer 26 is arranged in the premixing fermentation bin 14, and the feeding pipe 19 and the premixing fermentation bin 14 are both covered with a heat preservation layer. The heat preservation layer is arranged to preserve heat, and the electric heating plate is arranged to cooperate with the thermometer 26 to adjust and control the temperature in the premixing fermentation bin 14, so as to maintain a suitable fermentation temperature.
[0036] In order to improve the convenient maintenance performance of the device, referring to Figure 1 , the feeding screw 18 is provided with an inspection port 27, the feeding pipe 19 is provided with a high-pressure flushing pipe 20, the high-pressure flushing pipe 20 is provided with an electromagnetic valve 21, the high-pressure flushing pipe 20 is connected with an external high-pressure water source, and the high-pressure flushing pipe 20 is provided with at least two and distributed on the feeding pipe 19. The high-pressure flushing pipe 20 is arranged to facilitate the high-pressure water flow to be sprayed into the feeding pipe 19 from multiple directions, so as to facilitate the rapid cleaning of the inside of the feeding pipe 19, and the inspection port 27 is arranged to facilitate the inspection of the channel of the feeding screw 18.
[0037] The implementation principle is that: by setting the premixing fermentation bin 14 on the ordinary fermentation tank 11, the microbial solution in the fermentation tank 11 is pumped through the filter screen 17 to the premixing fermentation bin 14 through the pumping circulation pipe 16, the medium injection pipe 15 is matched, the microbial solution is injected into the medium in the premixing fermentation bin 14 through the inoculation mechanism, the preliminary fermentation is continuously carried out, the microbial concentration is proliferated, and the mixture after the preliminary fermentation is continuously introduced into the fermentation tank 11 through the pipeline by the continuous feeding unit, that is, the feeding screw 18, the mature microbial solution is continuously pumped into the premixing fermentation bin 14 and continuously fed into the medium, the preliminary mixed fermentation is completed in the premixing fermentation bin 14, and then the mature microbial solution is continuously and slowly introduced into the fermentation tank 11, the whole process is preliminarily fermented through the premixing fermentation tank 11, the microbial concentration is improved, the microbial environment is created, the disturbance to the internal environment of the fermentation tank 11 is reduced through continuous and slow feeding, and the imbalance of the bacterial population is reduced.
[0038] It should be noted that the connection relationship of the components not mentioned in the present application is defaulted to be the prior art, since it does not involve the invention point and is generally applied in the prior art, so the structure connection relationship is not described in detail.
Claims
1. A circulating device for d-ribose fermentation, comprising a fermentation tank (11), wherein a stirring mechanism (12), a temperature and pressure control mechanism, and a discharge port (13) are arranged in the fermentation tank (11), characterized in that, The fermentation tank (11) is provided with a premix fermentation bin (14), the premix fermentation bin (14) is provided with a culture medium injection pipe (15) and a strain circulating inoculation mechanism, the strain circulating inoculation mechanism comprises a pumping circulation pipe (16) arranged in the fermentation tank (11), a filter screen (17) is arranged at the connection between the pumping circulation pipe (16) and the fermentation tank (11), the upper end of the pumping circulation pipe (16) extends into the premix fermentation bin (14) and is provided with an inoculation mechanism at the end, a continuous feeding unit is arranged between the premix fermentation bin (14) and the fermentation tank (11), the continuous feeding unit comprises a motor-driven feeding screw (18) arranged at the bottom end of the premix fermentation bin (14), and the output end of the feeding screw (18) is connected to the fermentation tank (11) through a pipeline.
2. The circulation device for d-ribose fermentation according to claim 1, wherein The continuous feeding unit further comprises a feeding pipe (19) arranged at the output end of the feeding screw (18), and the feeding pipe (19) is arranged around the outer periphery of the fermentation tank (11) and extends into the fermentation tank (11) at the end.
3. The circulation device for d-ribose fermentation according to claim 2, wherein The feeding pipe (19) is provided with a high-pressure flushing pipeline (20), the high-pressure flushing pipeline (20) is provided with an electromagnetic valve (21), the high-pressure flushing pipeline (20) is connected to an external high-pressure water source, and the high-pressure flushing pipeline (20) is provided with at least two and is distributed on the feeding pipe (19).
4. The circulation device for d-ribose fermentation according to claim 1, wherein The inoculation mechanism comprises an inoculation disc (22) arranged at the upper end of the pumping circulation pipe (16), the inoculation disc (22) is provided with an array of inoculation needles (23), the inoculation needles (23) are arranged towards the bottom end of the premix fermentation bin (14), and the culture medium injection pipe (15) is arranged opposite to the inoculation needles (23).
5. The circulation device for d-ribose fermentation according to claim 4, wherein The premix fermentation bin (14) is provided with a circulating pump (24), the inlet end of the circulating pump (24) extends into the bottom end of the premix fermentation bin (14), and the outlet end of the circulating pump (24) extends into the upper end of the premix fermentation bin (14).
6. The circulation device for d-ribose fermentation according to claim 1, wherein The top of the premix fermentation bin (14) is provided with an electric heating plate (25), the electric heating plate (25) is electrically connected to a power supply, and the premix fermentation bin (14) is provided with a thermometer (26).
7. The circulation device for d-ribose fermentation according to claim 6, wherein The outer periphery of the feeding screw (18) is provided with an inspection opening (27).
8. The circulation device for d-ribose fermentation according to claim 2, wherein The feeding pipe (19) and the premix fermentation bin (14) are both wrapped with a heat preservation layer.