SiRNA annealing device

Abstract

The utility model discloses a SiRNA annealing device, and belongs to the technical field of life science. The SiRNA annealing device comprises a main body, a heating mechanism, a heat dissipation mechanism and a stirring mechanism, the main body comprises a base and a heat insulation cover, a square groove is formed in the upper end face of the base, a storage base is installed in the square groove, the heating mechanism is arranged in the base, and the heating mechanism comprises a pair of square plates; the opposite ends of the pair of square plates are connected with the two sides of the inner wall of the base correspondingly, a circular plate is installed between the pair of square plates, an electric heating coil is embedded in the circular plate, a variable resistor is installed at the bottom end of the interior of the base, and the variable resistor is electrically connected with the electric heating coil through a wire. A motor base is installed at the position, located on one side of the variable resistor, of the inner bottom end of the base, a first motor is installed in the motor base, the output end of the first motor and the outer portion of an adjusting knob of the variable resistor are both sleeved with synchronous wheels, and a synchronous belt is wound between the pair of synchronous wheels.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of life science technology, specifically is a SiRNA annealing device. BACKGROUND

[0002] SiRNA is a short RNA molecule that can inhibit the expression of specific genes by interfering with RNA. SiRNA annealing refers to the process of SiRNA molecules binding to target RNA. When SiRNA binds to target RNA, SiRNA will inhibit the translation and expression of target RNA through the mechanism of RNA-induced target RNA degradation. The principle of SiRNA annealing involves the complementary pairing of SiRNA and target RNA. SiRNA molecules are usually composed of two strands, the guide strand and the antisense strand. The guide strand is completely complementary to the sequence of the target RNA, while the antisense strand is complementary to the guide strand. When SiRNA binds to target RNA, the guide strand will complementarily pair with the sequence of the target RNA to form a double-stranded RNA complex. Oligonucleotide annealing is a common experimental technique widely used in biology, biochemistry, and genetics. It controls the temperature change of the primer to improve the affinity and pairing ability of the primer, so that it can specifically bind to the target sequence.

[0003] Based on the above, the present inventors found that there are the following problems: the current SiRNA annealing is roughly divided into three steps: heating to a set temperature, maintaining this temperature for a period of time, and reducing to room temperature. During the SiRNA annealing process, the temperature change needs to be precisely controlled. If the temperature is too high, the primer will not be stable in binding to the target RNA, and if the temperature is too low, the primer will bind to non-specific sequences.

[0004] Therefore, in view of the above, the existing structure and deficiencies are studied and improved, and a SiRNA annealing device is provided to achieve a more practical value. INVENTION CONTENTS

[0005] The purpose of the utility model is to provide a SiRNA annealing device to solve the problems raised in the background art.

[0006] In view of the above problems, the technical solution proposed by the utility model is:

[0007] The utility model provides a kind of SiRNA annealing device, including main body, heating mechanism, heat dissipation mechanism and stirring mechanism, the main body includes base and heat insulation cover, the upper end surface of the base is equipped with square groove, the inside of the square groove is installed with storage seat, the heating mechanism is arranged in the inside of base, and heating mechanism includes a pair of square plate, a pair of the square plate opposite end is connected with the inner wall two sides of base respectively, and the circular plate is installed between the square plate, the inside of the circular plate is embedded with electric heating coil, the inside bottom end of base is installed with variable resistor, the variable resistor is electrically connected between electric heating coil by wire, the inside bottom end of base is installed with motor base on the side of variable resistor, the first motor is installed in the inside of motor base, the output end of the first motor and the adjusting knob outside variable resistor are all sleeved with synchronous wheel, a pair of the synchronous wheel is wound with synchronous belt.

[0008] Further, the upper end surface of one of the square plates is installed with a temperature sensor.

[0009] The beneficial effects of the above further scheme are that by installing the temperature sensor, the temperature inside the base is monitored in real time.

[0010] Further, the heat dissipation mechanism includes a mounting seat, the back surface of the base is provided with a mounting groove, the mounting seat is installed in the inside of the mounting groove, and two pairs of supports are installed in the inside of the mounting seat, a second motor is installed between a pair of the supports, and the output end of the second motor is drivingly connected with a fan blade.

[0011] The beneficial effects of the above further scheme are that by the cooperation of the second motor and the fan blade, the number of the second motor is two, when the two second motors are started, the fan blade is driven to rotate to push the air to form an air current, so that the surrounding air is quickly circulated, and the heat inside the base is taken away, thereby facilitating the subsequent reduction of the annealing solution to room temperature.

[0012] Further, a pair of round holes are formed in the front surface and the back surface of the mounting seat at the position of the fan blade, and a filter screen is installed in the inside of each round hole.

[0013] The beneficial effects of the above further scheme are that by installing the filter screen in the inside of the round hole, the dust in the air is simply filtered when the air is circulated.

[0014] Further, the stirring mechanism includes an electric telescopic rod, the electric telescopic rod is installed on the top surface of the heat insulation cover, the movable end of the electric telescopic rod extends to the outside through the heat insulation cover, and a fixing seat is installed, a third motor is installed in the inside bottom end of the fixing seat, a stirring shaft is drivingly connected to the output end of the third motor, the bottom end of the stirring shaft extends to the outside through the fixing seat, and stirring blades are installed on the both sides of the bottom end of the stirring shaft.

[0015] The beneficial effect of the above further scheme is that, by installing the electric telescopic rod, the downward movement of the fixing seat is facilitated when the electric telescopic rod is started, the cross section of the fixing seat is circular, and the diameter of the fixing seat and the length of the pair of stirring blades are both smaller than the diameter of the container containing the annealing solution, so that the stirring blades can enter the container under the action of the downward movement of the fixing seat, by installing the third motor, the stirring shaft is driven to rotate when the third motor is started, and the annealing solution in the container is stirred under the action of the pair of stirring blades.

[0016] Further, the outer wall of the base is provided with a first connecting seat on one side, the second connecting seat is rotatably connected in the first connecting seat, and the outer side wall of the heat insulation cover is fixedly connected to one side of the second connecting seat.

[0017] The beneficial effect of the above further scheme is that, by the cooperation of the first connecting seat, the second connecting seat and the heat insulation cover, the heat insulation cover is conveniently opened upward, and then the container containing the annealing solution is placed on the storage seat.

[0018] Further, the front surface of the base is provided with a plurality of air vents.

[0019] The beneficial effect of the above further scheme is that, by providing the air vents on the front surface of the base, when the heat dissipation mechanism works, the surrounding air is quickly circulated, and the heat in the base is discharged through the air vents.

[0020] Further, one side of the top surface of the base is inclined, and a control panel is embedded and installed in the inside of the one side of the top surface, the control panel is provided with a frequency converter inside, and the control panel is electrically connected to the electric heating coil, the temperature sensor, the variable resistor, the first motor, the second motor, the electric telescopic rod and the third motor through wires.

[0021] The beneficial effect of the above further scheme is that, under the cooperation of the control panel and the electric heating coil, the temperature sensor, the first motor, the second motor, the electric telescopic rod and the third motor, the control panel can control the opening and closing of the electric heating coil, the temperature sensor can transmit the monitored temperature data to the control panel, the control panel displays the temperature and analyzes whether heating or heat dissipation is needed according to the set temperature value, the control panel can control the opening and closing of the first motor, the second motor, the electric telescopic rod and the third motor, and the frequency converter is arranged in the control panel, so that the power of the first motor is adjusted through the control panel.

[0022] Compared with the prior art, the SiRNA annealing device has the advantages that: the SiRNA annealing device is provided with a storage seat, a plurality of through grooves are formed in the storage seat, so that the container containing the annealing solution can be placed on the storage seat, an electric heating coil is embedded in the circular plate, heat is generated when the electric heating coil is powered on, and the container containing the annealing solution is provided with a heat source, so that the heating purpose is achieved, the variable resistor is used in cooperation with the electric heating coil, the resistance of the variable resistor is changed, the current of the electric heating coil is adjusted, and then the heating power is changed, so that the heating temperature is accurately adjusted and controlled, and the first motor, the synchronous wheel, the synchronous belt and the variable resistor are used in cooperation, when the first motor works, the output end of the first motor rotates, and the adjusting knob of the variable resistor is driven to rotate under the action of the synchronous wheel and the synchronous belt, the resistance value of the variable resistor is automatically changed, manual adjustment is not needed, and the heating power is automatically controlled. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 A three-dimensional structure schematic view of the SiRNA annealing device is provided.

[0024] Figure 2 An explosion three-dimensional structure schematic view of the SiRNA annealing device is provided.

[0025] Figure 3 An explosion three-dimensional structure schematic view of the heating mechanism of the SiRNA annealing device is provided.

[0026] Figure 4 A side cross-sectional structure schematic view of the mounting seat of the SiRNA annealing device is provided.

[0027] Figure 5 A front cross-sectional structure schematic view of the heat insulation cover of the SiRNA annealing device is provided.

[0028] In the drawing: 100, main body; 1001, base; 1002, storage seat; 1003, air hole; 1004, first connecting seat; 1005, second connecting seat; 1006, heat insulation cover; 1007, control panel; 200, heating mechanism; 2001, square plate; 2002, circular plate; 2003, electric heating coil; 2004, temperature sensor; 2005, variable resistor; 2006, motor seat; 2007, first motor; 2008, synchronous wheel; 2009, synchronous belt; 300, heat dissipation mechanism; 3001, mounting seat; 3002, support; 3003, second motor; 3004, fan blade; 400, stirring mechanism; 4001, electric telescopic rod; 4002, fixing seat; 4003, third motor; 4004, stirring shaft; 4005, stirring blade. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the present application.

[0030] Please refer to Figures 1-5 The utility model provides a technical scheme: a kind of SiRNA annealing device, including main body 100, heating mechanism 200, heat dissipation mechanism 300 and stirring mechanism 400, main body 100 includes base 1001 and heat insulation cover 1006, the upper end surface of base 1001 is equipped with square groove, square groove is equipped with article holder 1002 in its inside, heating mechanism 200 is set to the inside of base 1001, and heating mechanism 200 includes a pair of plates 2001, a pair of plates 2001 opposite end is connected with the inner wall of base 1001 two sides respectively, and circular plate 2002 is installed between a pair of plates 2001, electric heating coil 2003 is embedded and installed in the inside of circular plate 2002, variable resistor 2005 is installed at the inside bottom end of base 1001, variable resistor 2005 and electric heating coil 2003 are electrically connected by wire, motor base 2006 is installed at the inside bottom end of base 1001 on the side of variable resistor 2005, first motor 2007 is installed in the inside of motor base 2006, the output end of first motor 2007 and the adjusting knob outside variable resistor 2005 are all equipped with synchronous pulley 2008, synchronous belt 2009 is wound between a pair of synchronous pulley 2008, by installing article holder 1002, and several through slots are set on article holder 1002, so as to place the container with annealing solution on article holder 1002, by embedding and installing electric heating coil 2003 in circular plate 2002, when electric heating coil 2003 is powered, heat is generated, provides heat source for the container with annealing solution, realizes heating purpose, by the cooperation of variable resistor 2005 and electric heating coil 2003, variable resistor 2005 changes its resistance, adjusts the current of electric heating coil 2003, and then changes its heating power, realizes accurate control to heating temperature, when first motor 2007 works, its output end rotates, and under the action of synchronous pulley 2008 and synchronous belt 2009, the adjusting knob of variable resistor 2005 is rotated, automatically changes the resistance of variable resistor 2005, without manual adjustment, realizes automatic control heating power.

[0031] Clearly, the described embodiments are merely a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments of the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0032] Please refer to Figures 1-5 The utility model provides a technical scheme: one of square board 2001's upper end face is equipped with temperature sensor 2004, and the heat dissipation mechanism 300 includes the mounting seat 3001, and the back surface of base 1001 is equipped with the installation groove, and the mounting seat 3001 is installed in the inside of installation groove, and the inside of mounting seat 3001 is equipped with two pairs of support 3002, and a pair of support 3002 is equipped with second motor 3003 between, and the output end of second motor 3003 is connected with fan blade 3004 in drive mode, and the front and back of mounting seat 3001 are all equipped with a pair of round holes at fan blade 3004, and the inside of round hole is all equipped with filter screen, and the stirring mechanism 400 includes electric telescopic handle 4001, and electric telescopic handle 4001 is installed in the top surface of heat insulation cover 1006, and the movable end of electric telescopic handle 4001 extends to outside through heat insulation cover 1006, and is equipped with fixed base 4002, and the inside bottom end of fixed base 4002 is equipped with third motor 4003, and the output end of third motor 4003 is connected with stirring shaft 4004 in drive mode, and the bottom end of stirring shaft 4004 extends to outside through fixed base 4002, and the bottom end both sides of stirring shaft 4004 are all equipped with stirring blade 4005, and through the installation temperature sensor 2004, the temperature of the inside of base 1001 is monitored in real time, when starting two second motor 3003, drives fan blade 3004 to rotate and thus promotes air to form airflow, so that the surrounding air is rapidly circulated, and the heat of the inside of base 1001 is taken away, so that the annealing solution is conveniently reduced to room temperature subsequently, and when the inside of round hole is equipped with filter screen, when air circulation, the dust in the air is simply filtered, when starting electric telescopic handle 4001, makes fixed base 4002 move down, the cross section of fixed base 4002 is circular, and the diameter of fixed base 4002 and the length of a pair of stirring blade 4005 are all less than the diameter of the container with annealing solution, so that stirring blade 4005 can enter the inside of container under the action that fixed base 4002 moves down, when starting third motor 4003, drives stirring shaft 4004 to rotate, and under the action of a pair of stirring blade 4005, the annealing solution in the inside of container is stirred.

[0033] Clearly, the described embodiments are merely a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments of the present application, all the other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0034] Please refer to Figures 1-5 The utility model provides a technical scheme: the outer wall of base 1001 one side is equipped with first connecting seat 1004, the inside rotation of first connecting seat 1004 is connected with second connecting seat 1005, and one side of second connecting seat 1005 is fixedly connected with the outside wall of heat insulation cover 1006, the bottom end of heat insulation cover 1006 is in line with the upper end of base 1001, the front of base 1001 is equipped with a plurality of air holes 1003, and the top surface one side of base 1001 is inclined, and the inside of top surface one side of base 1001 is embedded with control panel 1007, the inside of control panel 1007 is equipped with frequency converter, and control panel 1007 is electrically connected between electric heating coil 2003, temperature sensor 2004, first motor 2007, second motor 3003, electric telescopic rod 4001 and third motor 4003 through wire, through the cooperation of first connecting seat 1004, second connecting seat 1005 and heat insulation cover 1006, heat insulation cover 1006 is conveniently opened upwards, then the container with annealing solution is placed on the container holder 1002, the air hole 1003 is set up on the front of base 1001, when heat dissipation mechanism 300 works, the surrounding air is circulated quickly, the heat in the inside of base 1001 is discharged through air hole 1003, control panel 1007 can control the opening and closing of electric heating coil 2003, temperature sensor 2004 can transmit the monitored temperature data to control panel 1007, control panel 1007 displays temperature and judges whether heating or heat dissipation is needed according to the set temperature value, and control panel 1007 can control the opening and closing of first motor 2007, second motor 3003, electric telescopic rod 4001 and third motor 4003.

[0035] Specifically, the working principle of the SiRNA annealing device is as follows: in use, the temperature is pre-set through the control panel 1007, then the heat insulation cover 1006 is lifted upwards, and then the container containing the annealing solution is placed on the container seat 1002. When the electric heating coil 2003 is powered on, heat is generated to provide a heat source for the container containing the annealing solution, so as to achieve the purpose of heating. Through the cooperation of the variable resistor 2005 and the electric heating coil 2003, the variable resistor 2005 adjusts the current of the electric heating coil 2003 by changing its resistance, and then changes the heating power, so as to accurately control the heating temperature. When the first motor 2007 works, its output end rotates and drives the adjustment knob of the variable resistor 2005 to rotate under the action of the synchronous wheel 2008 and the synchronous belt 2009, so as to automatically change the resistance value of the variable resistor 2005 without manual adjustment, so as to realize automatic control of the heating power. Through the installation of the temperature sensor 2004, the temperature inside the base 1001 is monitored in real time. During the heating process, the electric telescopic rod 4001 is started to make the fixed seat 4002 move downwards, so that the stirring blade 4005 can enter the container under the action of the downward movement of the fixed seat 4002. The third motor 4003 is started to drive the stirring shaft 4004 to rotate and stir the annealing solution in the container under the action of a pair of stirring blades 4005, so as to improve the heating efficiency. When the temperature reaches the pre-set temperature, the frequency converter is arranged inside the control panel 1007, so that the power of the first motor 2007 is reduced through the control panel 1007, and the temperature at this time is maintained for a set holding time. When the set holding time is reached, the first motor 2007 is turned off and the second motor 3003 is started through the control panel 1007 to drive the fan blade 3004 to rotate and push the air to form an air flow, so as to make the surrounding air flow quickly, and the heat in the base 1001 is discharged through the air holes 1003, so as to facilitate the annealing solution to be cooled to room temperature.

Claims

1. A SiRNA annealing apparatus, characterized by, The utility model provides a kind of heating device, including main body (100), heating mechanism (200), heat dissipation mechanism (300) and stirring mechanism (400), the main body (100) includes base (1001) and heat insulation cover (1006), the upper end surface of the base (1001) is equipped with square groove, the inside of the square groove is installed with object seat (1002), the heating mechanism (200) is set to the inside of base (1001), and heating mechanism (200) includes a pair of square plate (2001), a pair of the square plate (2001) opposite end is connected with the inner wall two sides of base (1001) respectively, and the circular plate (2002) is installed between the square plate (2001), the inside of the circular plate (2002) is embedded with electric heating coil (2003), the inside bottom end of the base (1001) is installed with variable resistor (2005), the variable resistor (2005) and electric heating coil (2003) are electrically connected by wire, the inside bottom end of the base (1001) is installed with motor base (2006) on the side of variable resistor (2005), the inside of the motor base (2006) is installed with first motor (2007), the output end of the first motor (2007) and the adjusting knob outside of variable resistor (2005) are all covered with synchronous wheel (2008), a pair of the synchronous wheel (2008) is wound with synchronous belt (2009).

2. The SiRNA annealing device of claim 1, wherein, The upper end surface of one of the square plate (2001) is installed with temperature sensor (2004).

3. The SiRNA annealing device of claim 1, wherein, The heat dissipation mechanism (300) includes mounting seat (3001), the back of the base (1001) is equipped with mounting groove, the mounting seat (3001) is installed in the inside of mounting groove, and the inside of the mounting seat (3001) is installed with two pairs of supports (3002), a pair of the support (3002) is installed with second motor (3003) between, the output end of the second motor (3003) is drivingly connected with fan blade (3004).

4. The SiRNA annealing device of claim 3, wherein, The front and back of the mounting seat (3001) are equipped with a pair of round holes at the fan blade (3004), and the inside of the round hole is installed with filter screen.

5. The SiRNA annealing device of claim 4, wherein, The stirring mechanism (400) includes electric telescopic rod (4001), the electric telescopic rod (4001) is installed on the top surface of heat insulation cover (1006), and the movable end of the electric telescopic rod (4001) extends to the outside through heat insulation cover (1006) and is installed with fixed seat (4002), the inside bottom end of the fixed seat (4002) is installed with third motor (4003), the output end of the third motor (4003) is drivingly connected with stirring shaft (4004), the bottom end of the stirring shaft (4004) extends to the outside through the fixed seat (4002), and the bottom end of the stirring shaft (4004) is installed with stirring blade (4005) on both sides.

6. The SiRNA annealing device of claim 5, wherein, The outer wall side of the base (1001) is provided with a first connecting seat (1004), the inside of the first connecting seat (1004) is rotatably connected with a second connecting seat (1005), one side of the second connecting seat (1005) is fixedly connected with the outer side wall of a heat insulation cover (1006), and the bottom end of the heat insulation cover (1006) is in close contact with the upper end of the base (1001).

7. The SiRNA annealing device of claim 6, wherein, The front of the base (1001) is provided with a plurality of air holes (1003).

8. The SiRNA annealing device of claim 7, wherein, The top side of the base (1001) is inclined, and a control panel (1007) is embedded and arranged in the inside of the top side of the base (1001), the inside of the control panel (1007) is provided with a frequency converter, and the control panel (1007) is electrically connected with the electric heating coil (2003), the temperature sensor (2004), the first motor (2007), the second motor (3003), the electric telescopic rod (4001) and the third motor (4003) through wires.

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