Improved culture bottle special for mycoplasma culture
By improving the design of mycoplasma culture bottles and adopting anti-adsorption coatings, breathable membranes, and anti-splash devices, the problems of insufficient sealing, ventilation, and anti-adsorption performance of traditional culture bottles have been solved, thereby improving the safety and reliability of mycoplasma culture and reducing the risk of cross-contamination.
Patent Information
- Application Number
- CN202520225613.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-13
- Publication Date
- 2025-12-16
- Estimated Expiration
- 2035-02-13
AI Technical Summary
Traditional culture bottles struggle to balance sealing, ventilation, and anti-adsorption properties, resulting in insufficient mycoplasma culture efficiency and reliability, and a high risk of cross-contamination.
An improved mycoplasma culture bottle was designed, made of transparent or semi-transparent glass or PET material, with an anti-adsorption coating on the inner wall, a breathable membrane assembly on the cap, solid and liquid culture media at the bottom, and equipped with an anti-splash device. The breathable membrane has a pore size of 0.1μm to 0.45μm to prevent cross-contamination.
It improves the safety and reliability of mycoplasma culture, reduces the risk of cross-contamination, extends the culture cycle, and has a simple structure that is easy to scale up for production.
Smart Images
Figure CN223674636U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to the field of bioengineering technology, and relates to a special culture bottle for improved mycoplasma culture. BACKGROUND
[0002] Mycoplasma is a kind of special cell wall-free microorganism. Due to the small size, strong permeability and strong adhesion of mycoplasma, cross contamination is easily caused in conventional culture devices, and the culture conditions are relatively harsh. The traditional culture bottle is difficult to balance in terms of sealing, ventilation, anti-adsorption performance and the like, and often leads to insufficient culture efficiency and reliability. Therefore, there is an urgent need for a culture bottle capable of realizing ventilation in a closed state, reducing mycoplasma adhesion and being easy to operate and observe, to meet the needs in scientific research and production. SUMMARY
[0003] The utility model discloses in order to make up for the insufficient of prior art, provided a kind of special culture bottle for improved mycoplasma culture, by improving bottle body material, optimizing inner wall coating and setting ventilation membrane component, improve the safety, reliability and visual degree of mycoplasma culture process, while effectively reduce the risk of cross contamination. The utility model is realized through the following technical schemes: the utility model provides a kind of special culture bottle for improved mycoplasma culture, including bottle body and bottle cap, bottle body upper side bottle mouth perimeter inner side and outer side are provided with thread structure, i.e. bottle mouth inner side screw thread and bottle mouth outer side screw thread;Bottle body is transparent or translucent;Thread structure is matched in bottle cap inner, and bottle cap is installed on bottle body by the thread structure of bottle body outer side;Bottle cap is provided with ventilation membrane component, and ventilation membrane component is composed of at least one layer of microporous filter membrane and support frame, and the pore size of the microporous filter membrane of ventilation membrane component is between 0.1 μm and 0.45 μm;Bottle body inner wall surface is provided with anti-adsorption coating;Solid culture medium is built-in in bottle body bottom, and the upper surface of the solid culture medium is inclined to bottle bottom, and the inclined surface of solid culture medium is filled with liquid culture medium between bottle body side wall;Anti-splashing device is installed in bottle body by bottle mouth inner side screw thread, and anti-splashing device is inverted circular table, and it is hollow, i.e. the upper and lower bottom surfaces can be ventilated;The bottom surface of the circular table with larger area is arranged on the upper, and the bottom surface with smaller area is arranged on the lower.
[0004] Preferably, the bottle body is cylindrical, the bottle wall is smooth, and the bottle body is made of one of glass and PET transparent material.
[0005] Preferably, when the solid culture medium is arranged, the non-solidified solid culture medium is first poured into the bottle body, and the bottle body is inclined to a certain angle before the solidification of the solid culture medium, so as to form an inclined surface.
[0006] Preferably, the carbon dioxide generating sheet is placed in the inner side of the circular table of the anti-splashing device.
[0007] The utility model has the advantages that:
[0008] The anti-absorption coating is arranged on the inner wall of the bottle body, so that the mycoplasma adhesion is reduced.
[0009] The gas permeable film assembly is arranged on the bottle cap, and the sterile gas permeation is realized through the microporous filter film; the gas exchange is facilitated, and the sterility of the bottle environment is ensured.
[0010] The anti-splashing device is arranged at the bottle opening, so that the culture solution is prevented from splashing to the gas permeable film assembly at the bottle opening when the culture bottle is moved, tilted or shaken, and the culture medium is prevented from being polluted; the anti-absorption and cross-contamination effects of the culture bottle are improved, and the culture period is prolonged.
[0011] The culture bottle is simple in structure, easy to mass produce and capable of meeting the application requirements of scientific research and the biological pharmaceutical industry. BRIEF DESCRIPTION OF DRAWINGS
[0012] The utility model will be further described below in combination with the drawings;
[0013] Figure 1 It is the disassembled structure schematic view of the mycoplasma culture bottle of the utility model;
[0014] Figure 2 It is the section view of the mycoplasma culture bottle of the utility model;
[0015] Figure 3 It is the front view of the utility model;
[0016] In the drawing, 1 bottle body, 2 bottle cap, 3 gas permeable film assembly, 4 anti-splashing device, 5 bottle opening inner side thread, 6 bottle opening outer side thread, 7 protrusion, 8 solid culture medium, 9 liquid culture medium. DETAILED DESCRIPTION
[0017] In order to make the above-mentioned purpose, features and advantages of the utility model more apparent and easy to understand, the specific implementation of the utility model will be described in detail below in combination with the drawings. In the following description, a plurality of specific details are set forth in order to fully understand the utility model. However, the utility model can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without departing from the connotation of the utility model, so the utility model is not limited by the specific embodiments disclosed below.
[0018] The drawings are specific embodiments of a modified mycoplasma culture special culture bottle of the utility model. As shown in the drawings, Figure 2 The bottle cap 2 is connected with the bottle body 1 through the bottle opening outer side thread 6, the anti-splashing device 4 is connected with the bottle body 1 through the bottle opening inner side thread 5 and is installed on the inner side of the bottle body;
[0019] The embodiment includes a bottle body 1 and a bottle cap 2. The inner and outer sides of the upper side of the bottle mouth of the bottle body 1 are respectively provided with a threaded structure, that is, a bottle mouth inner side thread 5 and a bottle mouth outer side thread 6. The bottle body 1 is transparent or translucent, and is made of one of glass or PET transparent materials, so as to facilitate observation of the growth state of mycoplasma. The bottle body 1 is in a cylindrical shape, which is convenient for holding and operation. The bottle wall is smooth and easy to clean, thereby reducing the risk of pollution of the bottle body. The bottle body 1 is externally provided with scale lines and a label area, which are used for observing the turbidity of the mycoplasma culture solution and marking the information of the reagent in the bottle.
[0020] The bottle cap 2 is provided with a breathable film assembly 3, which is composed of at least one layer of microporous filter film and a support frame. The support frame is in a circular shape and is installed on the inner side of the bottle cap. The microporous filter film is fixedly installed on the inner side of the support frame. The breathable film assembly can effectively block bacteria and particulate matter and maintain the sterile state of the culture environment in the bottle. The pore size of the microporous filter film of the breathable film assembly is between 0.1 μm and 0.45 μm, which can intercept most bacteria and microorganisms. In actual application, filter films with different pore sizes can be replaced according to experimental requirements. The microporous filter film realizes sterile and breathable.
[0021] The inner wall of the bottle body 1 is sprayed or dipped with an anti-adhesion coating to reduce the aggregation and adhesion of mycoplasma, thereby improving the experimental accuracy. An anti-adhesion coating is arranged on the surface of the inner wall of the bottle body to reduce the adhesion of mycoplasma. The anti-adhesion coating includes polyvinyl alcohol, polytetrafluoroethylene or a mixed modified coating thereof, so as to prevent mycoplasma from forming a biofilm on the bottle wall and affecting the culture effect.
[0022] A solid culture medium 8 is built-in at the bottom of the bottle body 1. The upper surface of the solid culture medium is inclined to the bottle bottom, thereby increasing the effective surface area of the solid culture medium and facilitating observation of the adhesion and growth of mycoplasma on the inclined surface. When the solid culture medium is arranged, the bottle body is first tilted to a certain angle before the un-solidified solid culture medium is poured into the bottle body, so as to form an inclined surface.
[0023] The inclined surface of the solid culture medium 8 is filled with a liquid culture medium 9 between the bottle body 1 side wall. The solid and liquid culture media are arranged in the same bottle body, so that mycoplasma can not only adhere and grow on the surface of the solid culture medium, but also obtain more sufficient nutrient supply in the liquid culture medium environment.
[0024] The anti-splashing device 4 is installed in the bottle body 1 through the bottle mouth inner side thread 5. The anti-splashing device 4 is in an inverted circular truncated cone shape and is hollow, that is, the upper and lower bottom surfaces are breathable. The bottom surface with a larger area is arranged on the upper side, and the bottom surface with a smaller area is arranged on the lower side. The anti-splashing device is arranged at the bottle mouth, so as to prevent the culture solution from splashing to the bottle mouth breathable film assembly when the culture bottle is moved, tilted or shaken, thereby preventing pollution of the culture medium.
[0025] The anti-splashing device is placed with carbon dioxide generating sheets in the inner side of a circular table, so as to provide a suitable gas environment for mycoplasma.
[0026] The bottle cap 6 is a screwable bottle cap, and the bottle cap and the bottle body are detachably connected through the bottle opening outer side thread 6.
[0027] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement for part or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present application, and they should be covered in the scope of the claims and the specification of the present application.
Claims
1. A culture bottle for Mycoplasma culture, comprising a bottle body and a bottle cap, characterized in that, The inner side and the outer side of the upper side of the bottle body around the bottle mouth are respectively provided with a thread structure, i.e. a bottle mouth inner side thread and a bottle mouth outer side thread; the bottle body is transparent or semi-transparent; a thread structure matched with the outer side of the bottle body is arranged in the bottle cap, and the bottle cap is installed on the bottle body through the thread structure of the outer side of the bottle body; a breathable film assembly is arranged on the bottle cap, the breathable film assembly is composed of at least one layer of a microporous filter film and a support frame, the pore size of the microporous filter film of the breathable film assembly is between 0.1 μm and 0.45 μm; an anti-absorption coating is arranged on the inner wall surface of the bottle body; a solid culture medium is arranged in the bottom of the bottle body, the upper surface of the solid culture medium is inclined to the bottom of the bottle, and the inclined surface of the solid culture medium is filled with a liquid culture medium between the inclined surface and the side wall of the bottle body; a splash-proof device is installed in the bottle body through the bottle mouth inner side thread, the splash-proof device is in the shape of an inverted circular truncated cone, and is hollow, i.e. the upper and lower bottom surfaces are breathable; the bottom surface with a larger area of the circular truncated cone is arranged on the upper side, and the bottom surface with a smaller area is arranged on the lower side.
2. The improved culture bottle for cultivating Mycoplasma according to claim 1, wherein The bottle body is in the shape of a cylinder, the bottle wall is smooth, and the bottle body is made of one of glass and PET transparent materials.
3. The improved culture bottle for cultivating Mycoplasma according to claim 1, wherein A scale line and a label area are arranged on the outside of the bottle body.
4. The improved culture bottle for cultivating Mycoplasma according to claim 1, wherein A convex is arranged in the middle of the bottom of the bottle body, so that the bottom of the bottle body is in the shape of an arc.
5. The improved culture bottle for cultivating Mycoplasma according to claim 1, wherein When the solid culture medium is arranged, the bottle body is first tilted to a certain angle before the solid culture medium is solidified, so as to form an inclined surface.
6. The improved culture bottle for cultivating Mycoplasma only according to claim 1, wherein A carbon dioxide generating sheet is arranged in the inner side of the splash-proof device.
7. The improved culture bottle for cultivating Mycoplasma only according to claim 1, wherein A silica gel sealing ring is arranged on the inner side of the bottle cap.