Chemical substance analyzer for living brain

Abstract

The living brain chemical substance analyzer comprises at least one acquisition board, the acquisition board comprises an acquisition microcontroller module, a DAC conversion module, a potentiostat module, a detection module, an amplification filtering module and an ADC conversion module, and the acquisition microcontroller module is electrically connected with a central control board; the detection module obtains a working electrode signal to obtain a detection output signal, the amplification filtering module is electrically connected with the detection module, and the amplification filtering module obtains the detection output signal to obtain an amplification filtering output signal; the constant potential rectifier module is connected with the DAC conversion module; through cooperation of current and voltage conversion of the multi-level selectable conversion path and three-level amplification of the multi-level selectable conversion path, a suitable path can be flexibly selected for testing according to the use requirements of a test experiment, the test precision can be effectively improved, the electrochemical reaction test in different test environments can be flexibly adapted, and the test efficiency is improved. Noise can be effectively reduced, and signals are kept stable and accurate.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of chemical substance detection, especially in-vivo brain chemical substance analyzer. BACKGROUND

[0002] In recent years, the research on the change rule of chemical substances in physiological and pathological processes has become a frontier problem that people pay close attention to, which is mainly due to the fact that the real-time change information of chemical substances in vivo can directly reflect the chemical nature in physiological and pathological processes. For example, the changes of glucose and lactic acid in the brain can directly reflect the energy metabolism process in the brain; glutamic acid is one of the excitatory neurotransmitters in the central nervous system, and its existence level and change rule in the brain are directly related to important physiological phenomena such as learning and memory and neural plasticity.

[0003] Neurotransmitters are a class of specific chemical substances that produce signal transduction between chemical synapses of neurons, which are synthesized in neurons, released by presynaptic membrane, and immediately combined with corresponding postsynaptic membrane receptors to complete the function of neural information transmission. Neurotransmitters are closely related to human health, and play an important role in the regulation of physiological functions of the body such as learning, memory, neuronal plasticity, somatic movement and brain development. Therefore, the research on the detection of neurotransmitter release in vivo has important significance for understanding the operation mechanism of the brain, researching the pathogenesis of brain diseases, drug treatment and clinical basic research.

[0004] The research on brain chemical substances cannot be separated from the detection of brain chemical substances. Only accurate detection can better understand the performance and use of chemical substances. With the development of brain science research, in-situ precise analysis of neurochemical signals in the brain has gradually attracted high attention in the fields of neuroscience and analytical chemistry. Electrochemical analysis method usually has the advantages of high sensitivity, good selectivity, high time and space resolution, and is suitable for in-vivo analysis. However, the physiological environment of in-vivo is complex. On the one hand, different neurochemical substances often interfere with the analysis process, resulting in inaccurate analysis of the concentration of the measured substance. On the other hand, many physiological and pathological processes involve the simultaneous change of multiple neurochemical substances, and how to realize the simultaneous recording of multiple neurochemical substances at the same site is also a challenge for in-vivo electrochemical analysis method

[0005] Fast scan cyclic voltammetry (FSCV) is an in-situ electrochemical analysis method that has been developed and improved in recent decades. This method has high temporal and spatial resolution, and can achieve millisecond-level rapid analysis on a micron-scale spatial scale. At the same time, qualitative differentiation of different substances can be achieved according to the differences in the influence of the redox process of different substances on the electrode voltammetry curve peak shape, and the potential for simultaneous analysis of multiple substances exists. As an electrochemical scanning technology that can be used for online detection of brain chemicals, its outstanding advantage is that it has an enrichment effect on the detected substances, which can increase the size of the detection current. The detection current obtained by detecting brain chemicals needs to be further processed and analyzed, including conversion, compensation, amplification, filtering, etc. In addition, the traditional applicable methods include IT (detection current) and CV (detection current) and OCPT (detection voltage) and the like.

[0006] However, the above technical solutions at least have the following defects. In the test process, the performance of the traditional instrument is single, and only fixed single test performance can be used for detection, which greatly limits the measurement range. Especially when the current-voltage conversion and multi-stage amplification are performed, due to the design of the fixed circuit, the voltage signal obtained by detection is greatly affected by the experimental environment in subsequent processing, and it is difficult to adapt to different environmental electrochemical reaction tests and effectively reduce noise, so that the signal is difficult to maintain stable and accurate, the reliability of the test result is reduced, the difficulty of realizing high-precision rapid detection is great, and experimental comparison cannot be formed in time, a large amount of time is needed for experiments, which is time-consuming and laborious, the experimental efficiency is low, and it is difficult to meet the use requirements of experimental detection. Practical new type content

[0007] In view of the above situation, in order to overcome the defects of the prior art, the living brain chemical substance analyzer provided by the present application comprises at least one acquisition board, the acquisition board comprises an acquisition microcontroller module, a DAC conversion module, a constant potential instrument module, a detection module, an amplification and filtering module and an ADC conversion module, and the acquisition microcontroller module is electrically connected with a central control board.

[0008] The detection module obtains a working electrode signal to obtain a detection output signal, the amplification and filtering module is electrically connected with the detection module, and the amplification and filtering module obtains the detection output signal to obtain a filtering output signal.

[0009] The constant potential instrument module is connected with the DAC conversion module.

[0010] The constant potential instrument module comprises a potential unit and an electrode interface unit which are electrically connected in sequence, the electrode interface unit is electrically connected with the electrode wiring, and the electrode interface unit is electrically connected with the detection module.

[0011] Further preferably, the detection module comprises a current detection unit, a voltage detection unit and a path control unit, the path control unit is electrically connected with the current detection unit and the voltage detection unit respectively, the current detection unit obtains the working electrode signal to obtain a first detection signal, the voltage detection unit obtains the working electrode signal to obtain a second detection signal; the path control unit obtains the first detection signal or the second detection signal to obtain the detection output signal.

[0012] Further preferably, the current detection unit comprises a U3B transimpedance amplifier component, a U2B isolation component and a relay component;

[0013] The DAC conversion module is electrically connected with the U2B isolation component, and the U2B isolation component is electrically connected with the U3B transimpedance amplifier component;

[0014] The acquisition microcontroller module is electrically connected with the relay component, and the relay component is electrically connected with the U3B transimpedance amplifier component;

[0015] The U3B transimpedance amplifier component is electrically connected with the path control unit.

[0016] Further preferably, the voltage detection unit comprises a U1B follow-up component, and the U1B follow-up component is electrically connected with the path control unit.

[0017] Further preferably, the path control unit comprises an electronic switch component, the U3B transimpedance amplifier component and the U1B follow-up component are electrically connected with the electronic switch component respectively, and the electronic switch component is electrically connected with the acquisition microcontroller module.

[0018] Further preferably, the amplification and filtering module comprises a first-stage program-controlled amplification and filtering unit, a second-stage program-controlled amplification and filtering unit and a third-stage program-controlled amplification unit which are electrically connected in sequence;

[0019] The first-stage program-controlled amplification and filtering unit receives the detection output signal to obtain a first-stage amplification and filtering signal, the second-stage program-controlled amplification and filtering unit receives the first-stage amplification and filtering signal to obtain a second-stage amplification and filtering signal, and the third-stage program-controlled amplification unit receives the second-stage amplification and filtering signal to obtain a third-stage amplification signal.

[0020] Further preferably, the first-stage program-controlled amplification and filtering unit comprises a first-stage amplification component and a first-stage filtering component which are electrically connected in sequence, and the first-stage amplification component is electrically connected with the electronic switch component.

[0021] Further preferably, the second programmable amplification and filtering unit comprises a second amplification component and a second filtering component connected in sequence, and the second amplification component is electrically connected with the first filtering component.

[0022] Further preferably, the third programmable amplification unit comprises a third amplification component, the second filtering component is electrically connected with the third amplification component, and the third amplification component is electrically connected with the ADC conversion module.

[0023] Compared with the prior art, the utility model discloses cleverly, through the synergic cooperation of multilayer level selectable conversion passage current voltage conversion and multilayer level selectable conversion passage three-stage amplification, can according to the use demand of test experiment, flexible selection its suitable passage carries out test, not only can effectively improve the test precision, flexible adaptation to the electrochemical reaction test of different test environment, greatly increases the measurable measurement range, can also effectively reduce the noise, makes the signal keep stable, accurate, improves the reliability of test result, realizes the high-precision rapid detection, and still can carry out independent test through double channel, can compare two experiments simultaneously, saves a large amount of experimental time, effectively improves the experimental efficiency, and the practicality is stronger, better satisfy the use demand of experimental detection. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 It is the acquisition board structure schematic drawing of the utility model.

[0025] Figure 2 It is the analyzer detection schematic drawing of the utility model.

[0026] Figure 3 It is the detection module structure schematic drawing of the utility model.

[0027] Figure 4 It is the current detection unit structure schematic drawing of the utility model

[0028] Figure 5 It is the U2B isolation component and U3B transimpedance amplification component circuit schematic drawing of the utility model.

[0029] Figure 6 It is the relay component circuit schematic drawing of the utility model.

[0030] Figure 7 It is the voltage detection unit structure schematic drawing of the utility model.

[0031] Figure 8 It is the U1B follow-up component circuit schematic drawing of the utility model.

[0032] Figure 9 It is the passage control unit structure schematic drawing of the utility model.

[0033] Figure 10 is a schematic diagram of an electronic switch assembly circuit of the utility model.

[0034] Figure 11 is a schematic diagram of an amplification filtering module structure of the utility model.

[0035] Figure 12 is a schematic diagram of a first-stage program-controlled amplification filtering unit structure of the utility model.

[0036] Figure 13 is a schematic diagram of a first-stage amplification assembly circuit of the utility model.

[0037] Figure 14 is a schematic diagram of a first-stage filtering assembly circuit of the utility model.

[0038] Figure 15 is a schematic diagram of a second-stage program-controlled amplification filtering unit structure of the utility model.

[0039] Figure 16 is a schematic diagram of a second-stage amplification assembly circuit of the utility model.

[0040] Figure 17 is a schematic diagram of a second-stage filtering assembly circuit of the utility model.

[0041] Figure 18 is a schematic diagram of a third-stage program-controlled amplification unit structure of the utility model.

[0042] Figure 19 is a schematic diagram of a third-stage amplification assembly circuit of the utility model.

[0043] Figure 20 is a schematic diagram of a constant potential instrument module structure of the utility model.

[0044] Figure 21 is a schematic diagram of a potential unit circuit of the utility model.

[0045] Figure 22 is a schematic diagram of an electrode interface unit circuit of the utility model.

[0046] Figure 23 is a schematic diagram of a central control board structure of the utility model.

[0047] Figure 24 is a schematic diagram of a first-stage isolation module circuit of the utility model.

[0048] Figure 25 is a schematic diagram of a second-stage isolation module circuit of the utility model. DETAILED DESCRIPTION

[0049] The specific embodiments of the utility model are further described in detail below in combination with the drawings.

[0050] By Figures 1 to 25 Some embodiments of the present application relate to in vivo brain chemical analysis instrument, including at least one acquisition board 1, the acquisition board 1 includes acquisition microcontroller module 101, DAC conversion module 102, potentiostat module 103, detection module 104, amplification filter module 105, ADC conversion module 106, acquisition microcontroller module 101 electrically connected with central control board 4;

[0051] The detection module 104 obtains a working electrode signal to obtain a detection output signal, and the amplification filter module 105 is electrically connected with the detection module 104, and the amplification filter module 105 obtains the detection output signal to obtain a filter output signal.

[0052] The detection module 104 includes a current detection unit 1041, a voltage detection unit 1042 and a path control unit 1043, the path control unit 1043 is electrically connected with the current detection unit 1041 and the voltage detection unit 1042 respectively, the current detection unit 1041 obtains a working electrode signal to obtain a first detection signal, and the voltage detection unit 1042 obtains a working electrode signal to obtain a second detection signal; the path control unit 1043 obtains the first detection signal or the second detection signal to obtain a detection output signal.

[0053] In these embodiments, as Figures 1-10 As shown in the figure, at least one acquisition board 1 is electrically connected with the to-be-detected chemical substance 3 through the electrode wiring 2 to obtain a working electrode signal of the to-be-detected chemical substance 3, in the detection module 104, the working electrode signal (WE) is detected and obtained by the current detection unit 1041, and the current voltage conversion is carried out through a multi-level selectable conversion path to obtain a first detection signal, wherein the current is converted into voltage through a negative feedback circuit composed of an operational amplifier to measure.

[0054] The current signal collected is converted through the transimpedance amplifier, and after the current signal is converted, the noise is large, the noise is removed through the low-pass filtering of the multi-level selectable path, the signal is kept stable and accurate, and the working requirement of low frequency is met.

[0055] Further, the working electrode signal (WE) is detected and obtained by the voltage detection unit 1042 to obtain a second detection signal, which is used for detecting the voltage change of the to-be-detected chemical substance 3, wherein the voltage change is directly measured by U1B operational amplifier circuit. In order to prevent circuit noise crosstalk, the present application selects a high input impedance operational amplifier through a voltage measurement special path, and the data received from the sensor will not be distorted or degraded due to the access of the measurement device, so as to ensure that the signal remains in its original state.

[0056] Since the input range of the ADC conversion module 106 is wide, different electrochemical experiment methods and different solutions and experiment environments can cause a big difference in the reaction current, in order to measure a wider range of current and improve the measurement precision, different paths are used for each sensitivity level, and each path is provided with a high-precision feedback resistor.

[0057] It should be understood that the actual application of the operational amplifier will have a bias current flowing into the non-inverting input terminal of the operational amplifier, and the frequency response characteristic of the operational amplifier will also change with the change of the signal frequency, so the selected operational amplifier in the utility model has a small bias current, a small input capacitance and a relatively flat frequency response characteristic.

[0058] In the utility model, through the synergistic cooperation of the multi-level selectable conversion path current-voltage conversion, the multi-level selectable conversion path three-stage amplification and the multi-path selectable low-pass filter circuit, the suitable path for testing can be flexibly selected according to the use requirement of the test experiment, not only can effectively improve the test precision, flexibly adapt to the electrochemical reaction test of different test environments, greatly increase the testable measurement range, but also can effectively reduce the noise, keep the signal stable and accurate, improve the reliability of the test result, and through the independent test of the double channels (two acquisition boards 1 are independently operated), two experiments can be simultaneously compared, a large amount of experiment time is saved, the experiment efficiency is effectively improved, the practicality is stronger, and the use requirement of the experiment detection is better met.

[0059] In a further embodiment, the current detection unit 1041 detects the working electrode signal and performs current-voltage conversion, and sends the first detection signal to the subsequent circuit through the path control unit 1043; the voltage detection unit 1042 detects the working electrode signal and performs voltage following, and sends the second detection signal to the subsequent circuit through the path control unit 1043. The ADC conversion module 106 of the utility model uses a pseudo-differential input mode, when a common-mode interference is input, the common-mode interference is reduced when entering the ADC conversion module 106, and the high accuracy of the sampling data is ensured.

[0060] The current detection unit 1041 comprises a U3B transimpedance amplification component 10411, a U2B isolation component 10412 and a relay component 10413.

[0061] The DAC conversion module 102 is electrically connected with the U2B isolation component 10412, and the U2B isolation component 10412 is electrically connected with the U3B transimpedance amplifier component 10411.

[0062] The acquisition microcontroller module 101 is electrically connected to the relay assembly 10413, and the relay assembly 10413 is electrically connected to the U3B transimpedance amplifier assembly 10411.

[0063] The U3B transimpedance amplifier assembly 10411 is electrically connected to the path control unit 1043.

[0064] like Figures 4-6 As shown, the U2B isolation component 10412 is used to receive the analog voltage (VOUTB) sent by the DAC conversion module 102, ensuring that the non-inverting input of the op-amp is 0V. Specifically, the U2B isolation component 10412 includes a 1K resistor R94, a capacitor C109, a 1K resistor R7, a capacitor C14, and a U2B amplifier; the U3B transimpedance amplifier component 10411 is used to receive the working electrode signal (WE) and the output signal (AGND) of the U2B isolation component 10412. The U3B transimpedance amplifier component 10411 includes a U3B operational amplifier, an R8 resistor of 0R, an R6 resistor of 100R, an R10 resistor of 0R, an R14 resistor of 100R, a C29 capacitor, a 1K R19 resistor, a C21 capacitor, a 10K R17 resistor, a C19 capacitor, a 100K R13 resistor, a C28 capacitor, a 1M R9 resistor, a C17 capacitor, a 10M R11 resistor, a 100M R12 resistor, a 1000M R15 resistor, and an R5 resistor of 0R. The connection relationship of the above components is as follows: Figure 5 As shown, both the U2B and U3B amplifiers can be the precision amplifier AD8642ARZ manufactured by Analog Devices.

[0065] Furthermore, the acquisition microcontroller module 101 controls the path selection of the U3B transimpedance amplifier component 10411 through the relay component 10413. The relay component 10413 selects which path to use. Specifically, the relay component 10413 includes K2 signal relay, K3 signal relay, K4 signal relay, K5 signal relay, K7 signal relay, K8 signal relay, K9 signal relay, U4 driver, and U22 decoder. The connection relationship of these components is as follows: Figure 6As shown in the figure, the model of the signal relay can be IM03GR of TE Connectivity, the model of the U4 driver chip can be the driver chip TBD62083AFNG produced by Toshiba, and the model of the U22 decoder chip can be the decoder chip CD74HC238PWR produced by Texas Instruments (TI).

[0066] In some embodiments of the in vivo brain chemical substance analyzer, the voltage detection unit 1042 comprises a U1B follower component 10421 electrically connected to the channel control unit 1043.

[0067] In these embodiments, as shown in the figure, Figures 7-8 the detected working electrode signal is sent to the channel control unit 1043 through the U1B follower component 10421, which specifically comprises a 100R R4 resistor, a D1 diode, and a U1B amplifier. The connection relationship of the above components is as shown in the figure, Figure 8 wherein the model of the U1B amplifier can be the high-precision operational amplifier OPA2182 produced by Texas Instruments.

[0068] Through the above-mentioned voltage follower circuit, high input impedance can be achieved, and the influence on the signal source is very small. It is very suitable for use as an interface for extracting signals from a high-impedance source. The voltage follower has low output impedance, which can drive a lower-impedance load while maintaining the integrity of the signal. Low output impedance helps to reduce signal loss and distortion during transmission. Moreover, the voltage follower circuit does not amplify the signal, i.e., the output voltage is equal to the input voltage, which can buffer or isolate the signal source from the load, and also isolate the input and output, effectively avoiding the interference of the lower-level circuit on the upper-level signal source.

[0069] In some embodiments of the in vivo brain chemical substance analyzer, the channel control unit 1043 comprises an electronic switch component 10431 electrically connected to the U3B transimpedance amplifier component 10411 and the U1B follower component 10421, and electrically connected to the acquisition microcontroller module 101.

[0070] In these embodiments, as shown in the figure, Figures 9-10 the electronic switch component 10431 selects the channel under the control of the acquisition microcontroller module 101, and specifically comprises a U5 analog switch. The connection relationship of the above components is as shown in the figure, Figure 10As shown, the model of the U5 analog switch can be an analog switch chip ADG1419BRMZ produced by Analog Devices Inc.

[0071] Further, the U5 analog switch is controlled by the acquisition microcontroller module 101, and SA and SB are connected to pin 1 through the switch, and different modes (current detection unit 1041 or voltage detection unit 1042) are selected, and the corresponding channel is opened, and then the U5 analog switch is used to send the signal to the subsequent circuit.

[0072] In some embodiments of the living brain chemical substance analyzer, the amplification filter module 105 comprises a first programmable amplification filter unit 1051, a second programmable amplification filter unit 1052 and a third programmable amplification unit 1053 connected in sequence.

[0073] The first programmable amplification filter unit 1051 receives the detection output signal to obtain a first amplification filter signal, the second programmable amplification filter unit 1052 receives the first amplification filter signal to obtain a second amplification filter signal, and the third programmable amplification unit 1053 receives the second amplification filter signal to obtain a third amplification signal.

[0074] In these embodiments, as shown, Figure 11 The detection output signal is transmitted to the amplification filter module 105, and in the amplification filter module 105, the signal is amplified by the first programmable amplification filter unit 1051, the second programmable amplification filter unit 1052 and the third programmable amplification unit 1053 in sequence, and the first amplification filter signal, the second amplification filter signal and the third amplification signal are gradually transmitted, and then transmitted to the acquisition microcontroller module 101 through the ADC conversion module 106.

[0075] Further, the amplification filter module 105 is connected to the detection module 104, and is used for three-stage amplification of the first detection signal obtained by converting the current voltage in the current detection unit 1041, or three-stage amplification of the second detection signal obtained by voltage following in the voltage detection unit 1042, wherein each stage of amplification is amplified by a multi-stage selectable path, and the first two stages of amplification are connected to a low-pass filter with a multi-stage selectable path.

[0076] In the amplification filter module 105, the three-stage programmable amplification circuit is used, and the amplification multiple of each circuit can be 1 or 10, and the corresponding path is selected according to the test needs during the test.

[0077] It should be understood that the amplification path needs to be accurately designed with a feedback network, and the high-precision feedback resistor is used in the utility model to ensure accurate gain.

[0078] Specifically, the low-pass filter circuit is connected behind each of the first two programmable amplification circuits, and the Sallen-Key second-order low-pass filter is used in the utility model, and the noise performance thereof is generally better than that of a pure passive filter. Moreover, the Sallen-Key filter requires fewer components, including only two resistors, two capacitors and an operational amplifier, and the use of the operational amplifier enhances the performance stability of the filter, and the noise performance thereof is generally better than that of a pure passive filter. A plurality of Sallen-Key second-order units are connected in series to construct a high-order filter.

[0079] Through the cooperation of the first-stage programmable amplification filter unit 1051, the second-stage programmable amplification filter unit 1052 and the third-stage programmable amplification unit 1053, the stability of the amplified voltage signal is effectively improved, the amplified voltage signal can be simultaneously subjected to filtering and noise reduction processing, the accuracy of the amplified voltage signal is further improved, the amplified signal can be stabilized to the required amplitude value, the difficulty of realizing a high-speed and high-precision amplified voltage signal is effectively reduced, the demand of subsequent detection is better met, the accuracy of the chemical substance analysis result is greatly improved, and the application flexibility is higher, and the power consumption cost is reduced.

[0080] In some living brain chemical substance analyzers, the first-stage programmable amplification filter unit 1051 comprises a first-stage amplification component 10511 and a first-stage filter component 10512 which are sequentially and electrically connected, and the first-stage amplification component 10511 is electrically connected with the electronic switch component 10431.

[0081] In these embodiments, as shown in Figures 12-14 the first-stage amplification component 10511 performs optional multi-channel amplification and then performs filtering processing through the first-stage filter component 10512, and the first-stage amplification component 10511 specifically comprises an R39 resistor, a U7A amplifier, an R37 resistor, an R46 resistor, an R40 resistor, an R41 resistor and a U20 analog switch, and the specific connection mode of the above components is as shown in Figure 13 wherein the R39 resistor is 1K, the U7A amplifier is a high-precision operational amplifier OPA2182 produced by Texas Instruments, the R37 resistor is 100R, the R46 resistor is an NC resistor (NC represents no resistance, which is generally used for early debugging), the R40 resistor is 1K, the R41 resistor is 10K, and the U20 analog switch is an analog switch chip ADG1419BRMZ produced by Analog Devices Inc.

[0082] It should be understood that, in Figure 13In the middle, U20 is an electronic analog switch, and the SA and SB pins are connected to the 1 pin. When SA is selected, the gain is -1, and the signal is amplified by 1 times. When SB is selected, the gain is -10, and the signal is amplified by 10 times. The left part is an amplification circuit: there are two amplification paths, which are controlled and selected by U20 and connected to the output pin 1. The amplified signal of the input signal AMP_OUT_QZ is AMP_OUT4. Moreover, U7A, R39, R40, and R41 form a typical inverting amplifier, and R39, R40, and R41 are feedback resistors, which determine the gain.

[0083] Further, the first filtering assembly 10512 specifically includes a 0R R16 resistor (for isolation and convenience of early debugging), a 6.19K R25 resistor, an 8.45K R27 resistor, a U21 analog switch, a U7B amplifier, a U8 analog switch, a 0R R26 resistor (for isolation and convenience of early debugging), and the specific connection relationship of the above components is as shown in Figure 14 The model of the U21 analog switch and the U8 analog switch is ADG1408YRUZ designed and manufactured by Texas Instruments (TI), and the model of the U7B amplifier is a high-precision operational amplifier OPA2182 produced by Texas Instruments.

[0084] It should be understood that different filter paths have different cutoff frequencies through the multi-channel low-pass filter circuit, and U8 and U21 are electronic analog switches controlled by pins 1, 16, and 15 to control S1-S8. Before AD acquisition, noise needs to be removed to keep the signal stable and accurate. The noise performance of the Sallen-Key second-order low-pass filter used in the utility model is usually better than that of a pure passive filter, and fewer components are required to build a high-order filter.

[0085] In some living brain chemical substance analyzers, the second program-controlled amplification and filtering unit 1052 includes a second amplification assembly 10521 and a second filtering assembly 10522 connected in sequence, and the second amplification assembly 10521 is electrically connected with the first filtering assembly 10512.

[0086] In these embodiments, as shown in Figures 15-17 After the optional multi-channel amplification of the second amplification assembly 10521, the optional multi-channel filtering processing of the second filtering assembly 10522 is performed, and the second amplification assembly 10521 specifically includes a 1K R48 resistor, a U28A amplifier, a 100R R36 resistor, a NC R44 resistor (NC represents a non-connected resistor, which is disconnected here, and is generally used for early debugging), a 10K R50 resistor, a U24 analog switch, a NC R47 resistor, and a 1K R51 resistor. The specific connection mode of the above components is as shown inFigure 16 As shown in the figure, the model of the U28A amplifier is a high-precision operational amplifier OPA2182 produced by Texas Instruments, and the model of the U24 analog switch is an analog switch chip ADG1419BRMZ produced by Analog Devices Inc. For details, refer to the working principle of the aforementioned first-stage amplification component 10511.

[0087] Further, the second-stage filtering component 10522 specifically includes a 6.19K R42 resistor, an 8.45K R43 resistor, a U26 analog switch, a U28B amplifier, a U23 analog switch, a 0R R45 resistor (for isolation and convenient early debugging), and the specific connection relationship of the above components is as shown in the figure Figure 17 As shown in the figure, the model of the U26 analog switch and the U23 analog switch is a chip ADG1408YRUZ designed and manufactured by Texas Instruments (TI), and the model of the U28B amplifier is a high-precision operational amplifier OPA2182 produced by Texas Instruments. For details, refer to the working principle of the aforementioned first-stage filtering component 10512.

[0088] In some living brain chemical substance analyzers, the third-stage program-controlled amplification unit 1053 includes a third-stage amplification component 10531, the second-stage filtering component 10522 is electrically connected to the third-stage amplification component 10531, and the third-stage amplification component 10531 is electrically connected to the ADC conversion module 106.

[0089] In these embodiments, as shown in the figure Figures 18-19 After the third-stage amplification component 10531 performs optional multi-channel amplification, it is sent to the ADC conversion module 106. The third-stage amplification component 10531 specifically includes a 1K R53 resistor, a U9A amplifier, a 100R R63 resistor, a NC R52 resistor (NC represents a non-connected resistor, which is disconnected here, which is generally used for early debugging), a 10K R54 resistor, a U27 analog switch, a NC R49 resistor (NC represents a non-connected resistor, which is disconnected here, which is generally used for early debugging), and a 1K R55 resistor. The specific connection mode of the above components is as shown in the figure Figure 19 As shown in the figure, the model of the U9A amplifier is a high-precision operational amplifier OPA2182 produced by Texas Instruments, and the model of the U27 analog switch is an analog switch chip ADG1419BRMZ produced by Analog Devices Inc.

[0090] In some embodiments of the in vivo brain chemical analyzer, the potentiostat module 103 is electrically connected to the DAC conversion module 102.

[0091] In some embodiments of the in vivo brain chemical analyzer, the potentiostat module 103 is electrically connected to the DAC conversion module 102.

[0092] In some embodiments of the in vivo brain chemical analyzer, the potentiostat module 103 is electrically connected to the DAC conversion module 102. Figures 20-22 As shown in FIG. 3, the potentiostat module 103 receives the detection analog voltage sent by the DAC conversion module 102 and provides a detection voltage to the chemical substance 3 to be detected, and is connected to the electrode wire 2 through the electrode interface unit 1032 to electrically connect the chemical substance 3 to be detected.

[0093] As shown in FIG. 3, the potentiostat module 103 receives the detection analog voltage sent by the DAC conversion module 102 and provides a detection voltage to the chemical substance 3 to be detected, and is connected to the electrode wire 2 through the electrode interface unit 1032 to electrically connect the chemical substance 3 to be detected. Figure 21 As shown in FIG. 3, the potentiostat module 103 receives the detection analog voltage sent by the DAC conversion module 102 and provides a detection voltage to the chemical substance 3 to be detected, and is connected to the electrode wire 2 through the electrode interface unit 1032 to electrically connect the chemical substance 3 to be detected. The potentiostat module 103 specifically includes a 1K R96 resistor, a C113 capacitor, a 2.2K R3 resistor, a C15 capacitor, a U2A amplifier, a F1 self-resetting fuse, a C4 capacitor, a C3 capacitor, a 2.2K R2 resistor, and a 2.2K R1 resistor. The specific connection relationship of the above components is shown in FIG. 3. The U2A amplifier is a precision amplifier AD8642ARZ produced by Analog Devices, and the F1 self-resetting fuse is a BSMD0603-002-60V brand BHFUSE.

[0094] As shown in FIG. 3, the potentiostat module 103 receives the detection analog voltage sent by the DAC conversion module 102 and provides a detection voltage to the chemical substance 3 to be detected, and is connected to the electrode wire 2 through the electrode interface unit 1032 to electrically connect the chemical substance 3 to be detected. Figure 21 As shown in FIG. 3, the potentiostat module 103 receives the detection analog voltage sent by the DAC conversion module 102 and provides a detection voltage to the chemical substance 3 to be detected, and is connected to the electrode wire 2 through the electrode interface unit 1032 to electrically connect the chemical substance 3 to be detected. The potentiostat module 103 specifically includes a 1K R96 resistor, a C113 capacitor, a 2.2K R3 resistor, a C15 capacitor, a U2A amplifier, a F1 self-resetting fuse, a C4 capacitor, a C3 capacitor, a 2.2K R2 resistor, and a 2.2K R1 resistor. The specific connection relationship of the above components is shown in FIG. 3. The U2A amplifier is a precision amplifier AD8642ARZ produced by Analog Devices, and the F1 self-resetting fuse is a BSMD0603-002-60V brand BHFUSE.

[0094] As shown in FIG. 3, the potentiostat module 103 receives the detection analog voltage sent by the DAC conversion module 102 and provides a detection voltage to the chemical substance 3 to be detected, and is connected to the electrode wire 2 through the electrode interface unit 1032 to electrically connect the chemical substance 3 to be detected. Figure 21 As shown in FIG. 3, the potentiostat module 103 receives the detection analog voltage sent by the DAC conversion module 102 and provides a detection voltage to the chemical substance 3 to be detected, and is connected to the electrode wire 2 through the electrode interface unit 1032 to electrically connect the chemical substance 3 to be detected. The potentiostat module 103 specifically includes a 1K R96 resistor, a C113 capacitor, a 2.2K R3 resistor, a C15 capacitor, a U2A amplifier, a F1 self-resetting fuse, a C4 capacitor, a C3 capacitor, a 2.2K R2 resistor, and a 2.2K R1 resistor. The specific connection relationship of the above components is shown in FIG. 3. The U2A amplifier is a precision amplifier AD8642ARZ produced by Analog Devices, and the F1 self-resetting fuse is a BSMD0603-002-60V brand BHFUSE.

[0094] As shown in FIG. 3, the potentiostat module 103 receives the detection analog voltage sent by the DAC conversion module 102 and provides a detection voltage to the chemical substance 3 to be detected, and is connected to the electrode wire 2 through the electrode interface unit 1032 to electrically connect the chemical substance 3 to be detected. Figure 21 As shown in FIG. 3, the potentiostat module 103 receives the detection analog voltage sent by the DAC conversion module 102 and provides a detection voltage to the chemical substance 3 to be detected, and is connected to the electrode wire 2 through the electrode interface unit 1032 to electrically connect the chemical substance 3 to be detected. The potentiostat module 103 specifically includes a 1K R96 resistor, a C113 capacitor, a 2.2K R3 resistor, a C15 capacitor, a U2A amplifier, a F1 self-resetting fuse, a C4 capacitor, a C3 capacitor, a 2.2K R2 resistor, and a 2.2K R1 resistor. The specific connection relationship of the above components is shown in FIG. 3. The U2A amplifier is a precision amplifier AD8642ARZ produced by Analog Devices, and the F1 self-resetting fuse is a BSMD0603-002-60V brand BHFUSE.

[0095] Further, the electrode interface unit 1032 specifically includes a J3 interface, a J1 interface, and a J2 interface, and the specific connection relationship of the above-mentioned components is as shown in Figure 22 As shown, the CE and RE signals output by the DAC conversion module 102 are input from the J1 interface and the J2 interface respectively, and are transmitted to the chemical substance 3 to be detected through the electrode wire 2, and the working electrode signal (WE) tested is output from the J3 interface.

[0096] In some embodiments of the living brain chemical substance analyzer, the at least two collection boards 1 are respectively electrically connected to the central control board 4, and the central control board 4 includes a first isolation module 401 and a second isolation module 402, the first isolation module 401 is electrically connected to one of the collection boards 1, and the second isolation module 402 is electrically connected to the other of the collection boards 1.

[0097] In some embodiments of the living brain chemical substance analyzer, the first isolation module 401 includes a U3 isolation component 4011, a U4 isolation component 4012, a J3 protection component 4013, and a J4 protection component 4014, and the second isolation module 402 includes a U5 isolation component 4021, a U6 isolation component 4022, a J5 protection component 4023, and a J6 protection component 4024.

[0098] In these embodiments, as shown in Figures 23-25 Two collection boards 1 are respectively connected to the central control board 4 through the first isolation module 401 and the second isolation module 402, further improving the protection and isolation effect. Specifically, the U3 isolation component 4011 includes a U3 digital isolator, the U4 isolation component 4012 includes a U4 digital isolator, the J3 protection component 4013 includes a J3 electrostatic discharge protector, and the J4 protection component 4014 includes a J4 electrostatic discharge protector; the U5 isolation component 4021 includes a U5 digital isolator, the U6 isolation component 4022 includes a U6 digital isolator, the J5 protection component 4023 includes a J5 electrostatic discharge protector, and the J6 protection component 4024 includes a J6 electrostatic discharge protector, and the connection relationship of the above-mentioned components is as shown in Figures 24-25 The model of the electrostatic discharge protector is RCLAMP0524, and the model of the digital isolator is ADUM142D0BRQZ produced by Analog Devices (Analog Devices) Company.

[0099] It is understood that the ADUM142D0BRQZ is a digital isolator produced by Analog Devices, which ensures the accuracy and integrity of the signal is not disturbed, which can be used for electrical isolation between circuits, ADUM142D0BRQZ provides digital signal isolation between circuits, prevents high voltage or current interference from affecting sensitive microcontrollers or other logic circuits; ADUM142D0BRQZ contains multiple isolation channels, which can isolate multiple digital signals at the same time. RCLAMP0524P.TCT is a circuit protection device produced by Semtech, which is mainly used to suppress transient voltage and provide circuit protection, especially in the protection of sensitive electronic devices. It is usually used to protect high-speed data lines and signal lines from transient voltages such as electrostatic discharge (ESD), cable discharge event (CDE), electrical fast transient (EFT) and lightning-induced voltage surge.

[0100] The above specific embodiments further illustrate the purpose, technical scheme and beneficial effects of the present application, and it should be understood that the above is only a specific embodiment of the present application and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application shall be included within the scope of protection of the present application.

Claims

1. An in vivo brain chemical analyzer characterized by comprising: The application relates to a data acquisition device, which comprises at least one acquisition plate (1), the acquisition plate (1) comprising an acquisition microcontroller module (101), a DAC conversion module (102), a constant potential instrument module (103), a detection module (104), an amplification and filtering module (105), and an ADC conversion module (106), the acquisition microcontroller module (101) being electrically connected with a central control plate (4); The detection module (104) obtains a working electrode signal to obtain a detection output signal, the amplification and filtering module (105) being electrically connected with the detection module (104), and the amplification and filtering module (105) obtaining the detection output signal to obtain an amplification and filtering output signal; The constant potential instrument module (103) is connected with the DAC conversion module (102); The constant potential instrument module (103) comprises a potential unit (1031) and an electrode interface unit (1032) which are electrically connected in sequence, the electrode interface unit (1032) being electrically connected with an electrode wire (2), and the electrode interface unit (1032) being electrically connected with the detection module (104).

2. The in vivo brain chemical analyzer according to claim 1, wherein The detection module (104) comprises a current detection unit (1041), a voltage detection unit (1042) and a channel control unit (1043), the channel control unit (1043) being electrically connected with the current detection unit (1041) and the voltage detection unit (1042) respectively, the current detection unit (1041) obtaining a first detection signal from the working electrode signal, and the voltage detection unit (1042) obtaining a second detection signal from the working electrode signal; the channel control unit (1043) obtains the detection output signal from the first detection signal or the second detection signal.

3. The brain chemical analyzer in vivo according to claim 2, wherein The current detection unit (1041) comprises a U3B transimpedance amplifier assembly (10411), a U2B isolation assembly (10412) and a relay assembly (10413); The DAC conversion module (102) is electrically connected with the U2B isolation assembly (10412), and the U2B isolation assembly (10412) is electrically connected with the U3B transimpedance amplifier assembly (10411); The acquisition microcontroller module (101) is electrically connected with the relay assembly (10413), and the relay assembly (10413) is electrically connected with the U3B transimpedance amplifier assembly (10411); The U3B transimpedance amplifier assembly (10411) is electrically connected with the channel control unit (1043).

4. The brain chemical analyzer in vivo according to claim 3, wherein The voltage detection unit (1042) comprises a U1B follower assembly (10421), and the U1B follower assembly (10421) is electrically connected with the channel control unit (1043).

5. The on-line brain chemical analyzer according to claim 4, wherein The passage control unit (1043) includes an electronic switch assembly (10431), a U3B transimpedance amplifier assembly (10411), and the U1B follow-up assembly (10421), which are electrically connected to the electronic switch assembly (10431), respectively, and the electronic switch assembly (10431) is electrically connected to the acquisition microcontroller module (101).

6. The in vivo brain chemical analyzer according to claim 5, wherein The amplification filtering module (105) includes a first-stage program-controlled amplification filtering unit (1051), a second-stage program-controlled amplification filtering unit (1052), and a third-stage program-controlled amplification unit (1053) which are electrically connected in sequence. The first-stage program-controlled amplification filtering unit (1051) receives the detection output signal to obtain a first-stage amplification filtering signal, the second-stage program-controlled amplification filtering unit (1052) receives the first-stage amplification filtering signal to obtain a second-stage amplification filtering signal, and the third-stage program-controlled amplification unit (1053) receives the second-stage amplification filtering signal to obtain a third-stage amplification signal.

7. The brain chemical analyzer in vivo according to claim 6, wherein The first-stage program-controlled amplification filtering unit (1051) includes a first-stage amplification assembly (10511) and a first-stage filtering assembly (10512) which are electrically connected in sequence, and the first-stage amplification assembly (10511) is electrically connected to the electronic switch assembly (10431).

8. The on-line brain chemical analyzer according to claim 7, wherein The second-stage program-controlled amplification filtering unit (1052) includes a second-stage amplification assembly (10521) and a second-stage filtering assembly (10522) which are electrically connected in sequence, and the second-stage amplification assembly (10521) is electrically connected to the first-stage filtering assembly (10512).

9. The on-line brain chemical analyzer according to claim 8, wherein The third-stage program-controlled amplification unit (1053) includes a third-stage amplification assembly (10531), and the second-stage filtering assembly (10522) is electrically connected to the third-stage amplification assembly (10531), and the third-stage amplification assembly (10531) is electrically connected to the ADC conversion module (106).

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