Staining liquid staining device for DNA (Deoxyribonucleic Acid) ploidy analysis

Abstract

The utility model provides a staining solution staining device for DNA (Deoxyribonucleic Acid) ploidy analysis. The staining solution staining device comprises a solution storage assembly, a positioning assembly and a driving assembly, the liquid storage assembly comprises a shell, and a plurality of box bodies are connected to the upper portion of the inner side wall of the shell and used for containing stationary liquid and various staining solutions; a water tank is further connected to the upper portion of the inner side wall of the shell, a waste water tank is arranged at the inner bottom of the shell, water outlet pipes with valves are installed at the lower ends of each box body and the water tank, and each water outlet pipe is communicated with the waste water tank; the positioning assembly comprises a base, and a groove is formed in the middle of the base. During use, the fixing liquid and various staining solutions are arranged in the same shell, so that the operation range is reduced, a slide filled with sample cells is positioned and clamped through the positioning assembly, and finally, the sample cells are automatically fixed, washed, stained and washed through the driving assembly, so that the staining efficiency is improved, the manual operation difficulty is reduced, and the working efficiency is improved. And the pollution to the surrounding environment is reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of biotechnology, in particular to a DNA ploidy analysis's staining solution dyeing device. BACKGROUND

[0002] In DNA ploidy analysis, the staining solution plays a vital role. It can stain the DNA in the cell, allowing DNA of different ploidy states to be clearly distinguished under a microscope or other detection equipment.

[0003] In the prior art, the staining process of cells needs to be soaked in solution at least twice. After the preparation is finished, the sample is first immersed in a fixing solution for fixation, then taken out for washing, and then immersed in a staining solution for staining operation. According to the experimental requirements, if different cells need to be stained and marked, a restaining operation is required to enhance the staining effect or distinguish different cell components. When immersed in the solution, the slide needs to be kept as flat as possible to prevent the sample cells from falling off due to the influence of buoyancy. This process needs to be operated manually and is relatively complicated.

[0004] Therefore, we propose a DNA ploidy analysis's staining solution dyeing device to solve the problem that the staining process of sample cells is manually operated and is relatively complicated in the prior art. CONTENT OF THE UTILITY MODEL

[0005] In view of the above-mentioned shortcomings of the prior art, the purpose of the utility model is to provide a DNA ploidy analysis's staining solution dyeing device to solve the problem that the staining process of sample cells is manually operated and is relatively complicated in the prior art.

[0006] To achieve the above-mentioned purposes and other related purposes, the utility model provides a DNA ploidy analysis's staining solution dyeing device, which comprises a liquid storage assembly, a positioning assembly and a driving assembly.

[0007] The liquid storage assembly comprises a shell, a plurality of boxes are connected to the top of the inner side wall of the shell, and the boxes are used to place a fixing solution and a plurality of staining solutions. A water tank is also connected to the top of the inner side wall of the shell, and a wastewater tank is arranged at the bottom of the shell. A water outlet pipe with a valve is arranged at the lower end of each box and water tank, and the drainage port of each water outlet pipe is communicated with the wastewater tank.

[0008] The positioning assembly comprises a base arranged above the shell, a recess is arranged in the middle of the base, and a clamping block is elastically connected to the two sides of the recess.

[0009] The driving assembly is arranged at the rear end of the shell, and the top of the driving assembly is connected with the positioning assembly, which is used to control the movement of the positioning assembly between different boxes and water tanks.

[0010] Preferably, all the boxes are arranged in a row, the water tank is long and parallel to the straight line formed by all the boxes, and the top end of the water tank is provided with a plurality of flush heads corresponding to each box.

[0011] Preferably, the rear end side wall of the water tank is connected with a water supply pipe, each flush head is communicated with the water supply pipe, and one end of the water supply pipe is connected with a hose penetrating through the rear end of the water tank.

[0012] Preferably, the outer side wall of each clamping block is connected with a slide rod, the other end of each slide rod is connected with and penetrates through a sleeve, the other end of each sleeve is connected with the base, a spring is sleeved in the interior of the slide rod, and the penetrating part of the sleeve is provided with a sealing ring.

[0013] Preferably, the lower end of the groove is provided with a plurality of water-permeable holes for draining the groove and reducing the solution taken out.

[0014] Preferably, the driving assembly comprises a translation module, a lifter and a rocker.

[0015] The translation module comprises a slide rail connected with the outer side edge of the shell, the outer side wall of the slide rail is connected with a motor one, the output end of the motor one penetrates through the slide rail and is connected with a screw rod, the screw rod penetrates through a support matched with the slide rail, the screw rod is threadedly connected with the support, the inner top of the support is provided with a sliding groove, the side wall of the sliding groove is connected with an electric push rod, and the other end of the electric push rod is connected with a sliding block matched with the sliding groove.

[0016] The lower end of the rocker is connected with the base for driving the base to swing, the top of the lifter is connected with the sliding block, the lower end of the lifter is connected with the rocker, and the lifter is used for driving the positioning assembly to perform lifting operation through the rocker.

[0017] Preferably, the front end of the support is connected with a transmitter, the top side edge of the shell is provided with a plurality of receivers corresponding to each box, and the transmitter is matched with the receiver.

[0018] Preferably, the lifter is an electric telescopic rod, the rocker comprises a fixed block connected with the electric telescopic rod, the interior of the fixed block is provided with a motor two, the output end of the motor two is connected with a gear, the lower end of the gear is engaged with a rack, the lower end of the fixed block is provided with a movable groove matched with the rack, the lower end of the rack is connected with a top plate, and the top plate and the base are connected through a plurality of vertical thin rods.

[0019] As described above, the utility model discloses a DNA ploidy analysis's staining solution dyeing device has following beneficial effect: the utility model, through setting up and fix the liquid and multiple staining solution are in same shell, reduce the operation range, again through the positioning assembly to the slide that is equipped with sample cell is positioned and clamped, finally through the drive assembly makes sample cell automation and carries out the operation of fixing, washing, dyeing, washing, improves the dyeing efficiency, reduces the artificial operation difficulty, prevents the solution spilling, reduces the pollution to surrounding environment.

[0020] Meanwhile, the device is also provided with a rocker, after the slide with sample cells enters the solution, start motor two, motor two drives gear positive and negative rotation, makes the gear drive rack left and right translation swing, and the rack drives the base left and right swing through the top plate and the thin rod, and then makes the slide left and right swing, facilitates the uniform coverage of dye liquor to cells, improves the dyeing quality.

[0021] Therefore, the utility model effectively overcomes the various shortcomings in the prior art and has high industrial utilization value. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 It is shown as the perspective view of the DNA ploidy analysis's staining solution dyeing device of the utility model.

[0023] Figure 2 It is shown as the shell side sectional perspective view of the DNA ploidy analysis's staining solution dyeing device of the utility model.

[0024] Figure 3 It is shown as the Figure 1 Enlarged view of part A of the DNA ploidy analysis's staining solution dyeing device of the utility model.

[0025] Figure 4 It is shown as the base sectional perspective view of the DNA ploidy analysis's staining solution dyeing device of the utility model.

[0026] Figure 5 It is shown as the Figure 4 Enlarged view of part B of the DNA ploidy analysis's staining solution dyeing device of the utility model.

[0027] Figure 6 It is shown as the drive assembly perspective view of the DNA ploidy analysis's staining solution dyeing device of the utility model.

[0028] Figure 7 It is shown as the rocker sectional perspective view of the DNA ploidy analysis's staining solution dyeing device of the utility model.

[0029] Element number explanation

[0030] 1, liquid storage assembly; 10, box body; 11, water tank; 12, waste water tank; 13, water outlet pipe; 14, water delivery pipe; 15, hose; 16, flushing head; 17, shell;

[0031] 2, positioning assembly; 20, base; 21, groove; 22, clamping block; 23, sliding rod; 24, sleeve; 25, spring; 26, sealing ring;

[0032] 3, drive assembly; 30, translation module; 31, lifter; 32, rocker;

[0033] 301, slide rail; 302, motor one; 303, screw rod; 304, support column; 305, sliding groove; 306, electric push rod; 307, sliding block; 308, transmitter; 309, receiver;

[0034] 320, fixed block; 321, motor two; 322, gear; 323, rack; 324, movable slot; 325, top plate; 326, thin rod. DETAILED DESCRIPTION

[0035] The embodiments of the present application will be described in detail by specific embodiments, and those skilled in the art can easily understand other advantages and effects of the present application from the content disclosed in the specification.

[0036] Please refer to Figures 1 to 7 It should be understood that the structure, proportion, size, etc. shown in the drawings attached to the specification are only used to cooperate with the content disclosed in the specification for those skilled in the art to understand and read, and are not used to limit the implementation conditions of the present application, so they do not have technical significance. Any modification of structure, change of proportion relationship or adjustment of size, without affecting the effects and purposes that can be achieved by the present application, should still fall within the scope of the technical content disclosed by the present application. At the same time, the terms such as "up", "down", "left", "right", "middle" and "one" in the specification are only for the convenience of clear description, and are not used to limit the scope of the present application. The change or adjustment of the relative relationship, without substantially changing the technical content, is also considered as the scope of the present application.

[0037] As Figures 1-7 shown, the present application provides a DNA ploidy analysis staining device, which comprises a liquid storage assembly 1, a shell 17, a positioning assembly 2 and a drive assembly 3.

[0038] The liquid storage assembly 1 comprises a shell 17, a plurality of boxes 10 are connected above the inner side wall of the shell 17, which are used to place fixing liquid and various staining liquids; a water tank 11 is also connected above the inner side wall of the shell 17, which is used to wash the cells taken out from the boxes 10 in the water tank 11 to remove the excess fixing liquid and staining liquid. The inner bottom of the shell 17 is provided with a waste water tank 12, the lower end of each box 10 and water tank 11 is provided with a water outlet pipe 13 with a valve, and each water outlet pipe 13 is communicated with the waste water tank 12; the waste water in the boxes 10 and water tank 11 is discharged into the waste water tank 12 for centralized storage, which is convenient for subsequent cleaning.

[0039] The positioning assembly 2 comprises a base 20 above the shell 17, a recess 21 is formed in the middle of the base 20, and elastic clamping blocks 22 are connected on both sides of the recess 21. The positioning assembly 2 is used for clamping and positioning the slide with sample cells. In use, the slide with sample cells is placed in the recess 21, and the slide is clamped and positioned by the elastic clamping blocks 22 at both ends.

[0040] The driving assembly 3 is located at the rear end of the shell 17, and the top of the driving assembly 3 is connected with the positioning assembly 2, which is used to control the movement of the positioning assembly 2 between different boxes 10 and water tanks 11. The slide is sequentially subjected to the operation steps of fixing liquid, washing, staining liquid and washing, and according to actual needs, the steps of staining liquid and washing can also be added. The driving assembly 3 realizes automatic staining, which makes the staining process convenient and fast.

[0041] In an embodiment, please refer to Figures 1-2 All the boxes 10 are arranged in a row, the water tank 11 is in a strip shape and parallel to the straight line formed by all the boxes 10, and the top end of the water tank 11 is provided with a plurality of flushing heads 16 corresponding to each box 10. By arranging all the boxes 10 and water tanks 11 uniformly, the movement program steps of the driving assembly 3 are simplified, and maintenance is facilitated. The flushing head 16 is used for washing the slide taken out from the box 10 to remove the excess fixing liquid and staining liquid brought out from the slide.

[0042] In an embodiment, please refer to Figures 1-2 The rear end side wall of the water tank 11 is connected with a water inlet pipe 14, each flushing head 16 is communicated with the water inlet pipe 14, and one end of the water inlet pipe 14 penetrates through the rear end of the water tank 11 and is connected with a hose 15. By arranging the hose 15, the hose 15 is connected with an external water source in use to perform water inlet operation.

[0043] In an embodiment, please refer to Figures 4-5Each outer side wall of each card block 22 is connected with a slide rod 23, one end of each slide rod 23 is connected with and penetrates a sleeve 24, the other end of each sleeve 24 is connected with the base 20, the slide rod 23 is sleeved with a spring 25 in the sleeve 24, and the sleeve 24 is provided with a sealing ring 26 at the penetrated position. When placing the slide, an operator pushes the card block 22 to move outwardly relative to the base 20, the card block 22 drives the slide rod 23 to retract into the sleeve 24 and extrude the spring 25, the slide is placed in the groove 21, the card block 22 is released, and the slide rod 23 and the card block 22 are reset under the action of the spring 25, so that the card block 22 clamps and positions the slide from both ends of the slide.

[0044] In an embodiment, referring to Figure 4 , the lower end of the groove 21 is provided with a plurality of water-permeable holes for draining water in the groove 21 and reducing the solution from being taken out. The solution is prevented from entering the remaining staining solution, causing pollution and affecting the staining of the sample cells.

[0045] In an embodiment, referring to Figure 1 , Figure 3 and Figures 6-7 , the driving assembly 3 comprises a translation module 30, a lifter 31 and a rocker 32.

[0046] The translation module 30 comprises a slide rail 301 connected with the outer side edge of the shell 17, an outer side wall of the slide rail 301 is connected with a motor one 302, an output end of the motor one 302 penetrates the slide rail 301 and is connected with a screw rod 303, the screw rod 303 penetrates a support column 304 matched with the slide rail 301, and the screw rod 303 is threadedly connected with the support column 304, an inner top portion of the support column 304 is provided with a sliding groove 305, a side wall of the sliding groove 305 is connected with an electric push rod 306, and the other end of the electric push rod 306 is connected with a sliding block 307 matched with the sliding groove 305; in use, the motor one 302 is started, the motor one 302 drives the screw rod 303 to rotate, the screw rod 303 drives the support column 304 to slide on the slide rail 301, and then drives the sample cells to translate on the water tank 11, after a fixing solution or a certain staining solution is selected, the electric push rod 306 is started to move the sample cells above the box body 10 containing the selected solution. The translation module 30 realizes translation in four directions of front, back, left and right in the same plane.

[0047] The lower end of the rocker 32 is connected with the base 20 for driving the base 20 to rock, the top of the lifter 31 is connected with the sliding block 307, the lower end of the lifter 31 is connected with the rocker 32, and the lifter 31 is used for driving the positioning assembly 2 to perform lifting operation through the rocker 32.

[0048] In an embodiment, referring to Figure 3The front end of the support 304 is connected with a transmitter 308, and the top side of the shell 17 is provided with a receiver 309 corresponding to each box body 10, and the transmitter 308 is matched with the receiver 309.

[0049] In an embodiment, referring to Figure 1 、 Figure 3 and Figure 7 , the lifter 31 is an electric telescopic rod; the slide glass is controlled to go in and out of the box body 10 stably through the electric telescopic rod. The rocker 32 comprises a fixed block 320 connected with the electric telescopic rod, the inside of the fixed block 320 is provided with a second motor 321, the output end of the second motor 321 is connected with a gear 322, the lower end of the gear 322 is engaged with a rack 323, the lower end of the fixed block 320 is provided with a movable slot 324 matched with the rack 323, the lower end of the rack 323 is connected with a top plate 325, and the top plate 325 and the base 20 are connected through a plurality of vertical thin rods 326. After the slide glass with sample cells enters the solution, the second motor 321 is started, the second motor 321 drives the gear 322 to rotate forward and reversely, the gear 322 drives the rack 323 to translate left and right to swing, the rack 323 drives the base 20 to swing left and right through the top plate 325 and the thin rod 326, and then the slide glass swings left and right, so that the dyeing liquid uniformly covers the cells.

[0050] The specific use process of the utility model is as follows: the operator uniformly distributes sample cells on the slide glass, puts into the recess 21 of the base 20 and clamps and fixes through the clamping block 22, starts the first motor 302, the first motor 302 drives the screw rod 303 to rotate, the screw rod 303 drives the support 304 to move horizontally, the support 304 moves to the rear of the box body 10 containing the fixing liquid, starts the electric push rod 306, pushes the slide block 307 to move forward, the slide block 307 drives the slide glass to move above the fixing liquid, starts the electric telescopic rod, the slide glass enters the fixing liquid and is fixed for a period of time, so as to ensure that the cells remain stable during the dyeing process, after reaching the predetermined time, the electric telescopic rod is started reversely, the slide glass is taken out of the fixing liquid, the electric push rod 306 is started reversely, the slide block 307 moves backward, drives the slide glass to move above the water tank 11, the electric telescopic rod is started again, the slide glass is placed in the water tank 11, the flushing head 16 is opened to wash the slide glass, and the excess fixing liquid is washed away, then the electric telescopic rod is started reversely again, drives the slide glass to lift, according to the requirement, the box body 10 containing the specified dyeing liquid is selected, the above operation is repeated, and the dyeing operation is completed. When the slide glass is in the dyeing liquid, the rocker 32 is started, drives the slide glass to swing left and right, and the cells are dyed uniformly.

[0051] The above embodiments only exemplarily illustrate the principles and effects of the present application, and are not used to limit the present application. Any person skilled in the art can modify or change the above embodiments without departing from the spirit and scope of the present application. Therefore, all equivalent modifications or changes completed by those skilled in the art without departing from the spirit and technical thought disclosed by the present application should be covered by the claims of the present application.

Claims

1. A staining device for DNA ploidy analysis, characterized by comprising: Include: Liquid storage assembly (1), positioning assembly (2) and drive assembly (3); The liquid storage assembly (1) comprises a shell (17), a plurality of box bodies (10) are connected above the inner side wall of the shell (17), used for placing fixing liquid and various staining liquids; the inner side wall of the shell (17) is also connected with a water tank (11) above, and the inner bottom of the shell (17) is provided with a wastewater tank (12); the lower end of each box body (10) and water tank (11) is provided with a water outlet pipe (13) with valve; the drain outlet of each water outlet pipe (13) is communicated with the wastewater tank (12); The positioning assembly (2) comprises a base (20) located above the shell (17), a recess (21) is formed in the middle of the base (20), and the base (20) is elastically connected with a clamping block (22) on both sides of the recess (21); The drive assembly (3) is located at the rear end of the shell (17), and the top of the drive assembly (3) is connected with the positioning assembly (2), used for controlling the movement of the positioning assembly (2) between different box bodies (10) and water tanks (11).

2. The staining device for DNA ploidy analysis according to claim 1, wherein: All the box bodies (10) are arranged in a row, the water tank (11) is long strip-shaped and parallel to the straight line formed by all the box bodies (10), and the top end of the water tank (11) is provided with a plurality of flushing heads (16) corresponding to each box body (10).

3. The staining device for DNA ploidy analysis according to claim 2, wherein: The rear end side wall of the water tank (11) is connected with a water supply pipe (14), each flushing head (16) is communicated with the water supply pipe (14), and one end of the water supply pipe (14) penetrates the water tank (11) and is connected with a hose (15).

4. The staining device for DNA ploidy analysis according to claim 1, wherein: The outer side wall of each clamping block (22) is connected with a slide rod (23), the other end of each slide rod (23) is connected and penetrates a sleeve (24), the other end of each sleeve (24) is connected with the base (20), the slide rod (23) is sleeved with a spring (25) in the inside of the sleeve (24), and the sleeve (24) is provided with a sealing ring (26) at the penetrated part.

5. The staining device for DNA ploidy analysis according to claim 1, wherein: The lower end of the recess (21) is provided with a plurality of water permeable holes for draining water in the recess (21) and reducing the solution taken out.

6. The staining device for DNA ploidy analysis according to claim 1, wherein: The drive assembly (3) comprises a translation module (30), a lifter (31) and a rocker (32); The translation module (30) comprises a slide rail (301) connected with the outer side of the shell (17), a motor (302) connected with the outer side wall of the slide rail (301), an output end of the motor (302) penetrating the slide rail (301) and connected with a screw rod (303), the screw rod (303) penetrating a support (304) matched with the slide rail (301), and the screw rod (303) and the support (304) are screwed, the inner top of the support (304) is provided with a sliding groove (305), the side wall of the sliding groove (305) is connected with an electric push rod (306), and the other end of the electric push rod (306) is connected with a sliding block (307) matched with the sliding groove (305). The lower end of the rocker (32) is connected with the base (20) for driving the base (20) to swing, the top of the lifter (31) is connected with the sliding block (307), the lower end of the lifter (31) is connected with the rocker (32), and the lifter (31) is used for driving the positioning assembly (2) to perform lifting operation through the rocker (32).

7. The staining device for DNA ploidy analysis according to claim 6, wherein: The front end of the support column (304) is connected with the transmitter (308), the top side edge of the shell (17) is provided with the receiver (309) corresponding to each box body (10), and the transmitter (308) is matched with the receiver (309).

8. The staining device for DNA ploidy analysis according to claim 6, wherein: The lifter (31) is an electric telescopic rod; the rocker (32) comprises a fixing block (320) connected with the electric telescopic rod, an electric machine two (321) is arranged in the fixing block (320), the output end of the electric machine two (321) is connected with a gear (322), the lower end of the gear (322) is engaged with a rack (323), the lower end of the fixing block (320) is provided with a movable slot (324), the movable slot (324) is matched with the rack (323), the lower end of the rack (323) is connected with a top plate (325), and the top plate (325) and the base (20) are connected through a plurality of vertical thin rods (326).

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