Test paper for detecting sclerotinia rot of colza

Abstract

The utility model relates to the technical field of germ detection tools, and discloses test paper for detecting sclerotinia rot of colza, which comprises a test paper strip body, a packaging bag is coated outside the test paper strip body, the test paper strip body comprises a bottom plate, a sample adding end and a detection end, the sample adding end and the detection end are arranged on the bottom plate, and the detection end is provided with a detection line and a control line. The packaging bag is made of a transparent material, T-C marks are printed at the positions, corresponding to the detection line and the control line, of the packaging bag, a strip-shaped opening is formed in the position, located at the sample adding end of the test strip body, of the packaging bag, an easy-to-tear strip capable of blocking the strip-shaped opening is fixedly installed on the packaging bag, side edge strips are arranged on the two sides of the easy-to-tear strip, and the side edge strips are arranged on the two sides of the easy-to-tear strip. The sides, close to the packaging bag, of the easy-to-tear strip and the side edge strip are coated with glue, and the easy-to-tear strip and the side edge strip are pasted to the packaging bag through the glue. The utility model aims to solve the problem that the existing rape sclerotium detection test paper box is inconvenient to use and carry.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of disease detection tools, specifically relates to a test paper for detecting sclerotinia sclerotiorum of rape. BACKGROUND

[0002] The pathogen of sclerotinia sclerotiorum of rape is Sclerotinia sclerotiorum, which belongs to Ascomycota and Sclerotinia. This pathogen mainly affects the stems, leaves, flowers, silique and seeds of rape, resulting in significant yield and quality decline. Sclerotinia sclerotiorum of rape is widely distributed worldwide, especially in the middle and lower reaches of the Yangtze River and the southeast coastal areas of China, with a disease incidence of 10-30%, and up to 80% in severe cases. In addition to rape, Sclerotinia can also infect other crops such as lettuce, sunflower, carrot, soybean, broad bean and pea.

[0003] The current test paper box for detecting sclerotinia sclerotiorum of rape is made of hard plastic and has a storage bag. When using, the storage bag needs to be torn open first, and then the treated extraction solution is dropped into the sample addition hole of the test paper box. The operation is not convenient, and it is not convenient to carry. Therefore, we propose a test paper for detecting sclerotinia sclerotiorum of rape to solve the above problems. UTILITY MODEL CONTENT

[0004] The utility model aims to solve the problem of the current test paper box for detecting sclerotinia sclerotiorum of rape, which is not convenient to use and not convenient to carry.

[0005] To achieve the above technical purpose, the utility model adopts the following technical scheme:

[0006] A test paper for detecting sclerotinia sclerotiorum of rape, comprising a test paper strip body, the test paper strip body is coated with a packaging bag, the test paper strip body comprises a bottom plate and a sample addition end and a detection end arranged on the bottom plate, the detection end is provided with a detection line and a control line, the packaging bag is made of transparent material, "T" and "C" marks are printed on the packaging bag corresponding to the detection line and the control line, a strip-shaped opening is arranged on the sample addition end of the test paper strip body, and an easy-to-tear strip is fixedly installed on the packaging bag to block the strip-shaped opening.

[0007] Further limited, the two sides of the easy-to-tear strip are provided with side strips, the side of the easy-to-tear strip and the side strip close to the packaging bag is coated with glue, and the easy-to-tear strip and the side strip are pasted to the packaging bag through the glue. Such structure design, through the side strip easy-to-tear strip can be pasted more firmly with the packaging bag, can prevent the outside dust from entering in the unused state, affects the practicality of the test paper strip body.

[0008] Further limited, still include for depositing test strip body storage box, test strip body code in the storage box, the storage box includes box body and the cover plate rotationally installed on the box body, the upper end edge of the box body is fixedly installed with the magnet, the cover plate is equipped with the magnetic block matched with the magnet.

[0009] Further limited, the cover plate is fixedly installed with a fixed strip, and the fixed strip is provided with a clamping mechanism capable of pressing the edge of the packaging bag.

[0010] Further limited, the clamping mechanism comprises a plurality of elastic clamping strips and a plurality of rubber bosses, and the plurality of elastic clamping strips and the plurality of rubber bosses are fixedly installed on the fixed strip in uniform intervals.

[0011] Further limited, the packaging bag is made of polylactic acid material, and the upper and lower ends of the packaging bag are subjected to heat plastic sealing treatment.

[0012] The utility model with the above technical scheme has the following advantages:

[0013] The utility model discloses a kind of test papers for detecting sclerotinia sclerotiorum of rape, including packaging bag, test strip body and easy tear strip, test strip body is fixedly installed in packaging bag, and easy tear strip is fixedly installed on the upper end of packaging bag. BRIEF DESCRIPTION OF DRAWINGS

[0014] The utility model can be further illustrated by the non-limiting embodiments shown in the drawings.

[0015] Fig. 1 It is a structure schematic view of the utility model discloses a kind of test papers for detecting sclerotinia sclerotiorum of rape;

[0016] Fig. 2 It is a structure schematic view of the utility model discloses a kind of test papers for detecting sclerotinia sclerotiorum of rape in use state;

[0017] Fig. 3 It is a structure schematic view of the easy tear strip part in the utility model discloses a kind of test papers for detecting sclerotinia sclerotiorum of rape;

[0018] Fig. 4 It is a structure schematic view of the storage box part of the utility model discloses a kind of test papers for detecting sclerotinia sclerotiorum of rape;

[0019] Fig. 5 It is Fig. 4 Enlarged structure schematic view of the place A in figure.

[0020] The main element symbols are explained as follows:

[0021] 1, test strip body; 2, packaging bag;

[0022] 3, easy-to-tear strip; 31, side strip;

[0023] 4, storage box; 41, box body;

[0024] 42, cover plate; 421, fixing strip; 422, elastic clamping strip; 423, rubber bump. DETAILED DESCRIPTION

[0025] The utility model will be described in detail below in combination with the drawings and specific embodiments, it is to be explained that, in the drawings or description, similar or same parts all use same figure number, the implementation mode not drawn or described in the drawings, for the form known to those skilled in the art.Additionally, the direction of the embodiment mentioned in the language, for example, "up", "down", "top", "bottom", "left", "right", "front", "back" and the like, only refer to the direction of the drawing, and not be used to limit the protection scope of the utility model.

[0026] As Figs. 1-5 shown, the utility model discloses a test paper for sclerotinia sclerotiorum detection, including test strip body 1, test strip body 1 is covered with packaging bag 2, test strip body 1 includes bottom plate and sets up sample end and detection end on bottom plate, and its detection end is equipped with detection line and control line, and sample end is the end of test strip entering the sample liquid to be measured, and the preparation process of the sample liquid to be measured is as follows, first, take the plant tissue (about 0.05g, half of the size of a small fingernail cover) to be detected and grind with disposable plastic grinding stick, add DNA crude extraction liquid (3% PEG 2000+20mM NaOH+2mM EDTA, total volume is 300 μL), and crack and extract DNA (crack time can not exceed 5min);Second, use pipette to suck DNA extraction liquid (liquid is sucked to the thin black line of pipette, about 10 μL) to new centrifugal tube, then add upstream primer (final concentration 0.4 μM), downstream primer (final concentration 0.4 μM), probe (final concentration 0.12 μM), commercial RPA reaction Buffer A (Jun Nuode, article number A4631), commercial RPA reaction Buffer B (Jun Nuode, article number A4631) and commercial RPA reaction dry powder (Jun Nuode, article number A4631), hold temperature to 30-40 DEG C between 15min with hand or put in clothes pocket;Three, add about 400 μL sterilization ultrapure water to dilute the reaction liquid in the reaction tube.

[0027] The packaging bag 2 is transparent, facilitating observation of the detection end of the test strip body 1, and the packaging bag 2 is provided with T and C marks corresponding to the detection line and the control line, respectively; the packaging bag 2 is provided with a strip-shaped opening at the sample adding end of the test strip body 1; the packaging bag 2 is fixedly provided with a tearable strip 3 capable of sealing the strip-shaped opening; the packaging bag 2 is made of polylactic acid, which is a recyclable and environmentally friendly material; the upper and lower ends of the packaging bag 2 are subjected to heat sealing treatment; the tearable strip 3 is provided with side strips 31 on both sides thereof; and the tearable strip 3 and the side strips 31 are coated with glue on the side close to the packaging bag 2 and are attached to the packaging bag 2 by means of the glue.

[0028] The utility model also comprises a storage box 4 for storing the test strip body 1, the test strip body 1 is stacked in the storage box 4, the storage box 4 comprises a box body 41 and a cover plate 42 rotatably installed on the box body 41, a magnet is fixedly installed on the upper end edge of the box body 41, the cover plate 42 is provided with a magnetic block matched with the magnet, and when the cover plate 42 is placed on the box body 41, the magnet and the magnetic block are magnetically attracted to each other, thereby locking the cover plate 42 to prevent the test strip inside from falling off.

[0029] The cover plate 42 is fixedly provided with a fixing strip 421, the fixing strip 421 is provided with a clamping mechanism capable of pressing the edge of the packaging bag 2, the clamping mechanism comprises a plurality of elastic clamping strips 422 and a plurality of rubber blocks 423, the plurality of elastic clamping strips 422 and the plurality of rubber blocks 423 are fixedly and uniformly installed on the fixing strip 421, after the sample liquid to be detected is added to the sample adding end of the test strip body 1, the upper end of the packaging bag 2 can be clamped between the elastic clamping strips 422 and the fixing strip 421, at this time, the rubber blocks 423 are in close contact with the packaging bag 2, thereby increasing the friction between the packaging bag 2 and the fixing strip 421, so that the packaging bag 2 and the test strip body 1 are firmly locked on the cover plate 42.

[0030] The use method of the utility model is as follows:

[0031] Grind the plant tissue to be detected with a disposable plastic grinding rod, add DNA extraction solution, and lyse and extract DNA (the lysis time should not exceed 5 min);

[0032] Suck the DNA extraction solution into a new centrifugal tube with a straw, and then add upstream primers, downstream primers, probes, commercial RPA reaction Buffer A, commercial RPA reaction Buffer B, and commercial RPA reaction dry powder, hold the temperature between 30-40 DEG C for 15 min by hand or in a pocket.

[0033] Finally, add about 400 μL of sterilized ultrapure water to the reaction tube to dilute the reaction solution, so as to prepare the sample liquid to be detected for use.

[0034] The tear strip 3 on the packaging bag 2 is torn, and then the lower end of the packaging bag 2 is pushed to the rear of the test strip body 1, so that the sample adding end of the test strip body 1 is exposed, the sample adding end is inserted into the sample liquid to be measured by holding the packaging bag 2, then the lower end of the packaging bag 2 is reset, the sample adding end is located in the packaging bag 2, and the detection result can be obtained after standing for 3-5 min.

[0035] The oilseed rape sclerotinia detection test paper provided by the utility model is described in detail. The description of the specific embodiment is only used for helping to understand the method and the core idea of the utility model. It should be pointed out that, for ordinary skilled persons in the art, without departing from the principle of the utility model, the utility model can be improved and modified in many ways, and these improvements and modifications also fall within the protection scope of the utility model claim.

Claims

1. A test strip for detecting sclerotinia stem rot in rapeseed, comprising a test strip body (1), the test strip body (1) being covered by a packaging bag (2), the test strip body (1) comprising a base plate and a sample application end and a detection end disposed on the base plate, the detection end being provided with a detection line and a control line, characterized in that: The packaging bag (2) is made of transparent material. The packaging bag (2) is printed with "T" and "C" markings at the corresponding positions of the detection line and control line. The packaging bag (2) has a strip-shaped opening at the sample application end of the test strip body (1). The packaging bag (2) is fixedly installed with an easy-tear strip (3) that can seal the strip-shaped opening.

2. The test strip for detecting sclerotinia stem rot in rapeseed according to claim 1, characterized in that: The easy-tear strip (3) has side strips (31) on both sides. The easy-tear strip (3) and the side strips (31) are coated with glue on the side of the packaging bag (2). The easy-tear strip (3) and the side strips (31) are glued to the packaging bag (2).

3. The test strip for detecting sclerotinia stem rot in rapeseed according to claim 1, characterized in that: It also includes a storage box (4) for storing the test strip body (1), the test strip body (1) is placed in the storage box (4), the storage box (4) includes a box body (41) and a cover plate (42) rotatably mounted on the box body (41), a magnet is fixedly installed on the upper edge of the box body (41), and a magnetic block adapted to the magnet is provided on the cover plate (42).

4. The test strip for detecting sclerotinia stem rot in rapeseed according to claim 3, characterized in that: A fixing strip (421) is fixedly installed on the cover plate (42), and the fixing strip (421) is provided with a clamping mechanism that can press the edge of the packaging bag (2) tightly.

5. The test strip for detecting sclerotinia stem rot in rapeseed according to claim 4, characterized in that: The clamping mechanism includes multiple elastic clamping strips (422) and several rubber protrusions (423), which are all evenly spaced and fixedly installed on the fixing strip (421).

6. The test strip for detecting sclerotinia stem rot in rapeseed according to claim 1, characterized in that: The packaging bag (2) is made of polylactic acid, and the top and bottom ends of the packaging bag (2) are thermoplastic sealed.

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