Test strip for identifying gray mold
By integrating grinding, pyrolysis, and dilution functions, the test strip for identifying gray mold has solved the problem of inconvenient field testing, achieving simplified operation and efficient detection.
Patent Information
- Application Number
- CN202423151504.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-20
- Publication Date
- 2025-12-26
- Estimated Expiration
- 2034-12-20
AI Technical Summary
Current techniques for detecting gray mold are inconvenient to perform in the field, requiring various tools and complicated procedures.
A test strip for identifying gray mold was designed, comprising a shell, a grinding chamber, a reaction chamber, and a delivery channel, integrating grinding, lysis, and dilution functions, and utilizing a sliding plate and spikes to achieve direct sample detection.
It simplifies the field testing process, improves operational convenience and efficiency, and is suitable for rapid field testing.
Smart Images
Figure CN223723131U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to the technical field of test paper detection, and particularly relates to a gray mold identification test paper strip. BACKGROUND
[0002] Gray mold is an important disease that occurs on a variety of fruit and vegetable crops. The disease occurs early, spreads quickly, and lasts for a long time, and it harms various organs such as leaves, stems, flowers, and fruits, seriously threatening the yield and quality of crops. The disease is widespread in tomato-producing areas, and can cause 40% to 50% yield loss, or even absolute loss, when it is severe. Meanwhile, gray mold is also one of the devastating diseases in strawberry-producing areas, and it occurs widely in both open field and protected cultivation, generally causing 20% to 30% yield loss, or more than 50% when it is severe, or even absolute loss. Therefore, it is necessary to monitor Botrytis cinerea in real time in the field and in facility agriculture, and to prevent and control gray mold in the early stage.
[0003] When detecting the disease, a through slot is detected by test paper. In the prior art, the plant tissue is taken down for grinding, then DNA extraction solution is added, DNA is lysed and extracted, then the DNA extraction solution is sucked into a new centrifugal tube, primers, probes and other substances are added, then dilution is performed, and then the diluted sample is detected by a test paper strip. In the entire detection process, many tools are involved, and it is very inconvenient to detect in the field.
[0004] In view of the above defects in the prior art, the present application is developed through in-depth research. CONTENT OF THE UTILITY MODEL
[0005] The utility model aims at providing a gray mold identification test paper strip, which solves the problem of inconvenient operation of detecting plant pathogens in the field in the prior art.
[0006] To achieve the above technical purpose, the utility model adopts the following technical scheme:
[0007] A gray mold identification test paper strip comprises a shell and a detection test paper, the shell is internally formed with a mounting cavity for mounting the detection test paper, the shell is formed with a grinding cavity for grinding plant tissue and a reaction cavity, a conveying channel is arranged between the grinding cavity and the reaction cavity, an isolation membrane is arranged between the reaction cavity and the mounting cavity, a sliding plate for carrying the detection test paper is further arranged in the mounting cavity, the sliding plate is slidingly connected to the cavity bottom of the mounting cavity, a plurality of sharp spikes for piercing the isolation membrane are formed at one end of the sliding plate close to the reaction cavity, a driving block is arranged on one side of the sliding plate, a connecting plate is arranged between the driving block and the sliding plate, a through slot is formed on one side of the shell in sliding cooperation with the connecting plate, and the driving block is arranged outside the shell.
[0008] Further, the grinding cavity is provided with a cavity cover, and a grinding rod is movably arranged on the cavity cover; the grinding rod comprises a grinding head arranged in the grinding cavity, a pressing plate arranged above the cavity cover, and a connecting rod connecting the grinding head and the pressing plate, and a connecting ball is arranged on the connecting rod and connected with the cavity cover in a universal manner; the grinding rod is movably arranged on the cavity cover, and the grinding rod can be directly controlled to rotate and grind the sample by pressing.
[0009] Further, a plurality of clamping blocks are arranged on the side of the cavity cover and clamped with the cavity opening of the grinding cavity; the clamping blocks can make the cavity cover more stable.
[0010] Further, a liquid injection opening is arranged on the top of the shell and communicated with the reaction cavity, and a sealing plug is arranged at the liquid injection opening; the liquid injection opening can be used to inject the reaction liquid into the reaction cavity.
[0011] Further, a filter plate is arranged in the conveying channel, and a plurality of filter holes are arranged on the filter plate; the filter plate can be used to filter the residues in the grinding cavity.
[0012] Further, an installation groove for installing the test paper is arranged on the sliding plate, and an opening is arranged on one side of the installation groove close to the reaction cavity; a flow guide plate is arranged on one end of the sliding plate close to the reaction cavity and communicated with the installation groove, and the sharp needle is arranged at the front end of the flow guide plate; the flow guide plate can be used to guide the liquid to flow to the test paper.
[0013] Compared with the prior art, the moldy identification test paper strip can be used to grind, lyse DNA and dilute the plant sample on the shell, the isolation film can be punctured by the sharp needle arranged at the end of the sliding plate, the reaction liquid can be directly soaked on the test paper, and the detection is performed, so that the operation process is more convenient and the moldy identification test paper strip is more suitable for use in the field. BRIEF DESCRIPTION OF DRAWINGS
[0014] The moldy identification test paper strip can be further illustrated by the non-limiting embodiments shown in the drawings.
[0015] Figure 1 The moldy identification test paper strip can be further illustrated by the non-limiting embodiments shown in the drawings.
[0016] Figure 2 The moldy identification test paper strip can be further illustrated by the non-limiting embodiments shown in the drawings. Figure 1 The moldy identification test paper strip can be further illustrated by the non-limiting embodiments shown in the drawings.
[0017] Figure 3 The moldy identification test paper strip can be further illustrated by the non-limiting embodiments shown in the drawings. Figure 2 The moldy identification test paper strip can be further illustrated by the non-limiting embodiments shown in the drawings.
[0018] Figure 4The utility model discloses a connecting structure schematic diagram of cavity cover and grinding stick in the utility model.
[0019] Figure 5 The utility model discloses a structure schematic diagram of sliding plate.
[0020] The main element symbol explanation is as follows: shell 1, installation cavity 11, grinding cavity 12, reaction cavity 13, conveying channel 14, through slot 15, liquid injection port 16, detection test paper 2, isolation film 3, sliding plate 4, sharp 41, driving block 42, connecting plate 43, mounting groove 44, flow guide plate 45, cavity cover 5, clamping block 51, grinding stick 6, grinding head 61, pressing plate 62, connecting rod 63, connecting ball 631, sealing plug 7, filter plate 8. DETAILED DESCRIPTION
[0021] The utility model will be described in detail below in combination with the drawings and specific embodiment, need explaining, in the drawing or the description, similar or same part all use same figure number, the implementation mode that is not drawn or described in the drawing, for the form that the ordinary skill in the art knows. In addition, the direction language such as "up", "down", "top", "bottom", "left", "right", "front", "back" etc. mentioned in the embodiment, is only the direction of reference drawing, and not used to limit the protection scope of the utility model.
[0022] As Figures 1-5 As shown, a kind of gray mould identification test paper strip involved in the utility model, including shell 1 and detection test paper 2, shell 1 is formed with observation window for observing test paper, installation cavity 11 for installing detection test paper 2 is formed in shell 1, specifically, grinding cavity 12 for grinding plant tissue and reaction cavity 13 are formed on shell 1, conveying channel 14 is arranged between grinding cavity 12 and reaction cavity 13, conveying channel 14 is close to the upper portion of grinding cavity 12;Reaction cavity 13 and installation cavity 11 are equipped with isolation film 3, wherein isolation film 3 is PE film;Installation cavity 11 is also equipped with the sliding plate 4 of bearing detection test paper 2, sliding plate 4 is slidably connected at the cavity bottom of installation cavity 11, a plurality of sharp 41 that can pierce isolation film 3 is formed in the end of sliding plate 4 close to reaction cavity 13, driving block 42 is arranged on the side of sliding plate 4, connecting plate 43 is arranged between driving block 42 and sliding plate 4, the through slot 15 of sliding fit with connecting plate 43 is formed in the side of shell 1, and driving block 42 is at the outside of shell 1.Several anti-skid lines are formed on driving block 42, by pushing driving block 42, sliding plate 4 is moved to isolation film 3 and is pierced through isolation film 3 by sharp 41, so that reaction liquid is impregnated on detection test paper 2, and detection is carried out.
[0023] In order to facilitate the grinding of the plant sample, the cavity cover 5 is clamped on the grinding cavity 12, and the grinding rod 6 is movably arranged on the cavity cover 5; the grinding rod 6 comprises a grinding head 61 in the grinding cavity 12, a pressing plate 62 above the cavity cover 5, and a connecting rod 63 connecting the grinding head 61 and the pressing plate 62, and the connecting rod 63 is provided with a connecting ball 631 connected with the cavity cover 5. The specific operation method is as follows: after the plant sample is put into the grinding cavity 12, the cavity cover 5 is covered, the thumb is pressed on the pressing plate 62, then the thumb is shaken left and right, the grinding head 61 is controlled to grind the plant sample, in order to improve the grinding effect, the bottom of the grinding cavity 12 is spherical, the bottom of the grinding head 61 is also spherical, in addition, the top of the pressing plate 62 is spherical recessed, which is more suitable for the thumb, and slipping is avoided, after grinding, the cavity cover 5 can be opened, the DNA extraction solution is added into the grinding cavity 12, the DNA is extracted by lysis, then the extraction solution is transferred to the reaction cavity 13 through the conveying channel 14, specifically, the DNA extraction solution can be 3% PEG 2000+20mM NaOH+2mM EDTA.
[0024] In the embodiment, in order to make the installation of the cavity cover 5 more stable, avoid the movement of the cavity cover 5 during grinding, a plurality of clamping blocks 51 are formed on the side of the cavity cover 5, which are clamped with the cavity opening of the grinding cavity 12, and the edge of the cavity opening is formed with a clamping groove matched with the clamping block 51.
[0025] When the extraction solution enters the reaction cavity 13 through the conveying channel 14, upstream primers (final concentration 0.4 μM), downstream primers (final concentration 0.4 μM), probes (final concentration 0.12 μM), commercial RPA reaction Buffer A (Junnuode, item number A4631), commercial RPA reaction Buffer B (Junnuode, item number A4631) and commercial RPA reaction dry powder (Junnuode, item number A4631) need to be added into the reaction cavity 13, and after 30 min of reaction, they enter sterile ultrapure water for dilution, therefore, the liquid injection port 16 is formed on the top of the shell 1 and communicates with the reaction cavity 13, which is convenient for operation, and the sealing plug 7 is arranged at the liquid injection port 16 to avoid spilling.
[0026] In the embodiment, the filter plate 8 is arranged in the conveying channel 14, and a plurality of filter holes are formed in the filter plate 8. The filter plate 8 facilitates the filtration of residues in the grinding cavity 12.
[0027] In the embodiment, the slide plate 4 is formed with a mounting groove 44 for mounting the test paper 2, the test paper 2 and the mounting groove 44 can be connected by means of adhesion, an opening is formed on the side of the mounting groove 44 close to the reaction cavity 13; the end of the slide plate 4 close to the reaction cavity 13 is formed with a flow guide plate 45 communicated with the mounting groove 44, and the sharp 41 is located at the front end of the flow guide plate 45. After the sharp 41 pierces the isolation film 3, the reaction liquid can be soaked on the test paper 2 for detection, and the flow guide plate 45 can make the reaction liquid more easily soaked on the test paper 2.
[0028] The use method of the utility model is as follows: firstly, the plant sample is directly put into the grinding cavity 12 after being cut off, and is ground by the grinding rod 6, after grinding, the DNA extraction solution is added into the grinding cavity 12 to lyse and extract DNA, then the whole shell 1 can be inclined to the side of the reaction cavity 13, so that the extraction solution can flow into the reaction cavity 13, then the reaction liquid and the sterilized ultrapure water are added into the reaction cavity 13 in sequence, after reaction and dilution, the slide plate 4 is moved to the isolation film 3 by pushing the driving block 42, the isolation film is pierced by the sharp 41, so that the reaction liquid can be soaked on the test paper 2 for reaction detection.
[0029] The grey mould identification test paper provided by the utility model is described in detail. The description of the specific embodiment is only used for helping to understand the method and the core idea of the utility model. It should be pointed out that, for ordinary skilled persons in the technical field, the utility model can be improved and modified on the premise of not departing from the principle of the utility model, and these improvements and modifications also fall within the protection scope of the utility model claim.
Claims
1. A gray mold identification test strip, comprising a shell (1) and a detection test paper (2), an installation cavity (11) for installing the detection test paper (2) is formed in the shell (1), characterized in that: The shell (1) is formed with a grinding cavity (12) for grinding plant tissues and a reaction cavity (13), and a conveying channel (14) is arranged between the grinding cavity (12) and the reaction cavity (13); an isolation film (3) is arranged between the reaction cavity (13) and the mounting cavity (11); the mounting cavity (11) is further provided with a sliding plate (4) for bearing the test paper (2), the sliding plate (4) is slidingly connected to the bottom of the mounting cavity (11), one end of the sliding plate (4) near the reaction cavity (13) is formed with a plurality of sharp spikes (41) capable of piercing the isolation film (3), one side of the sliding plate (4) is provided with a driving block (42), a connecting plate (43) is arranged between the driving block (42) and the sliding plate (4), one side of the shell (1) is formed with a through slot (15) slidingly matched with the connecting plate (43), and the driving block (42) is located outside the shell (1).
2. A test strip for the identification of grey mould according to claim 1, characterized in that: The grinding cavity (12) is clamped with a cavity cover (5), and the cavity cover (5) is movably provided with a grinding rod (6); the grinding rod (6) comprises a grinding head (61) in the grinding cavity (12), a pressing plate (62) above the cavity cover (5), and a connecting rod (63) connecting the grinding head (61) and the pressing plate (62); and the connecting rod (63) is provided with a connecting ball (631) connected with the cavity cover (5) in all directions.
3. A test strip for the identification of grey mould according to claim 2, characterized in that: A plurality of clamping blocks (51) are further formed on the side edges of the cavity cover (5) and clamped with the cavity openings of the grinding cavity (12).
4. The test strip for identifying gray mold according to claim 1, characterized in that: The top of the shell (1) is further formed with a liquid injection port (16) communicated with the reaction cavity (13), and a sealing plug (7) is arranged at the liquid injection port (16).
5. The test strip for identifying gray mold according to claim 1, characterized in that: The conveying channel (14) is further provided with a filter plate (8), and a plurality of filter holes are formed in the filter plate (8).
6. The test strip for identifying gray mold according to claim 1, characterized in that: The sliding plate (4) is formed with a mounting groove (44) for mounting the test paper (2), and an opening is formed on one side of the mounting groove (44) near the reaction cavity (13); one end of the sliding plate (4) near the reaction cavity (13) is formed with a flow guide plate (45) communicated with the mounting groove (44), and the sharp spikes (41) are located at the front end of the flow guide plate (45).