Cryopreservation box with programmed cooling function

Abstract

The utility model provides a cryopreservation box with a programmed cooling function, and relates to the technical field of cryopreservation boxes. The cryopreservation box with the programmed cooling function comprises a box body, a cover body and a containing assembly, the cover body is hinged to the box body, the containing assembly is arranged in the box body and movably connected with the inner wall of the box body, a liquid storage cavity is formed between the bottom face of the containing assembly and the inner bottom face of the box body, and the liquid storage cavity is communicated with the box body. The box body is provided with a liquid injection port and a liquid discharge port which are communicated with the liquid storage cavity; a cooling assembly is further arranged on the outer wall of the box body and comprises a condensing box, an air extracting pump, an air extracting pipe and an air returning pipe, the air extracting pump is connected with the condensing box and the air extracting pipe, the other end of the air extracting pipe is communicated with the interior of the box body, and the air returning pipe is connected with the condensing box. The two ends of the air return pipe communicate with the interior of the condensation box and the interior of the box body correspondingly. And the cells can be directly cryopreserved after being cooled, so that the influence on the biological activity of the cells is reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of cryopreservation box, specifically, relate to a kind of cryopreservation box with program cooling function. BACKGROUND

[0002] Cryopreservation box is currently commonly used one kind of sample cryopreservation device, as the matching of cryopreservation tube in biology and medical experiment, provides bearing for cryopreservation tube. When cryopreservation is handled to cell, commonly used program cooling method is carried out cold storage treatment, specific steps are, first, cell cryopreservation tube is placed into program cooling box, then program cooling box is placed into-80 DEG C refrigerator, when cell liquid in cell cryopreservation tube is reduced to-80 DEG C, cell cryopreservation tube in program cooling box is taken out and is moved into cell cryopreservation box, then cell cryopreservation box is placed into liquid nitrogen and is carried out cold storage treatment.

[0003] However, in the cold storage treatment process, since the program cooling box and the cell cryopreservation box have many cell cryopreservation tubes, the staff needs to take and move the cell cryopreservation tubes many times, the program cooling box and the cell cryopreservation box are both integral structures, and every time a cryopreservation tube is taken, the temperature of other cryopreservation tubes will be lost, which not only easily causes the temperature in the cell cryopreservation tube to change, affecting the cooling and cold storage effect of the cells, but also the long operation time may affect the biological activity of the cells, and the staff's fingers are more likely to be frozen. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a kind of cryopreservation box with program cooling function, which can directly cryopreserve after cooling cell, reduce the influence on cell biological activity.

[0005] The utility model realizes the following technical scheme:

[0006] A kind of cryopreservation box with program cooling function, including box body, cover and placing assembly, the cover is hinged with the box body, the placing assembly is set in the box body and is movably connected with the inner wall of the box body, the bottom surface of the placing assembly and the inner bottom surface of the box body form liquid storage cavity, the box body is provided with liquid injection port and liquid discharge port communicated with the liquid storage cavity;The outer wall of the box body is also provided with cooling assembly, the cooling assembly includes condensing box, air pump, air suction pipe and air return pipe, the air pump is connected with the condensing box and the air suction pipe respectively, the other end of the air suction pipe is communicated with the inside of the box body, the two ends of the air return pipe are communicated with the condensing box and the inside of the box body respectively.

[0007] Further, the placing assembly includes placing rack and fixed plate, a plurality of through holes are formed in the placing rack, a plurality of fixed grooves corresponding to the through holes are formed in the fixed plate, and the fixed plate and the placing rack are detachably connected.

[0008] Further, the inner wall of the box body is provided with a sliding groove, two ends of the placing rack are installed in the sliding groove, and the bottom of the sliding groove is provided with an electric push rod, and the push rod end of the electric push rod is in contact with the bottom surface of the placing rack.

[0009] Further, a freeze tube is arranged in the through hole, and a tube cover of the freeze tube is clamped in the fixing groove.

[0010] Further, the bottom of the freeze tube is provided with a heat preservation sleeve, a sandwich layer is arranged between the inner and outer walls of the heat preservation sleeve, and a cold preservative is arranged in the sandwich layer.

[0011] Further, the bottom of the condensing box is provided with a refrigerating device.

[0012] Further, the box body is provided with an observation window.

[0013] The technical scheme of the utility model has at least the following advantages and beneficial effects:

[0014] In the utility model, the liquid storage cavity and the cooling assembly cooperate, not only can the cryopreserved cells be cooled, but also the cryopreserved cells after cooling can be directly cryopreserved, without the need of transferring the cryopreserved cells, avoiding the influence of frequent taking on the biological activity of the cryopreserved cells, and preventing the frostbite of the fingers of the workers. Through the placing assembly, the freeze tube can be placed in the box body for cooling and cryopreservation, and the placing assembly can be moved to adjust the height of the freeze tube, so that the freeze tube is in the refrigerating medium of the liquid storage cavity, and the cryopreservation effect of the cells is ensured. BRIEF DESCRIPTION OF DRAWINGS

[0015] In order to more clearly illustrate the technical scheme of the embodiment of the utility model, the following will briefly introduce the drawings needed to be used in the embodiment, and it should be understood that the following drawings only show some embodiments of the utility model, and should not be regarded as the limitation to the scope, and for the ordinary skilled in the art, other related drawings can be obtained without the creative labor on the basis of the drawings.

[0016] Figure 1 The cross-sectional view of the cryopreservation box with the programmed cooling function provided for the embodiment 1 of the utility model;

[0017] Figure 2 The cross-sectional view of the cryopreservation box with the programmed cooling function provided for the embodiment 1 of the utility model; Figure 1 The local enlarged view of A in the middle;

[0018] Figure 3 The structural schematic view of the fixing plate provided for the embodiment 1 of the utility model;

[0019] Figure 4 The front view of the cryopreservation box with the programmed cooling function provided for the embodiment 1 of the utility model.

[0020] Icon: 1-box, 2-cover, 3-put the frame, 4-plate, 5-slots, 6-electric push rod, 7-liquid injection port, 8-drainage, 9-liquid storage cavity, 10-condenser, 11-pump, 12-exhaust pipe, 13-back pipe, 14-refrigerator, 15-hole, 16-frozen tube, 17-heat preservation sleeve, 18-interlayer, 19-fixed slot, 20-observation window. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical scheme and advantages of the embodiments of the present application more clear, the technical scheme of the embodiments of the present application will be described clearly and completely below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. The components of the embodiments of the present application described and shown in the drawings here can be arranged and designed in various different configurations.

[0022] Therefore, the following detailed description of the embodiments of the present application provided in the drawings is not intended to limit the scope of the claimed present application, but only represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative labor are within the scope of protection of the present application.

[0023] It should be noted that: similar signs and letters represent similar items in the following drawings, therefore, once an item is defined in one drawing, it does not need to be further defined and explained in the subsequent drawings.

[0024] In the description of the present application, it should be noted that if the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship of the product in use, only for the convenience of describing the present application and simplifying the description, and it is not intended or implied that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, therefore, it cannot be understood as a limitation on the present application.

[0025] In the description of the utility model, still need to explain, unless another explicit provision and limitation, if appear the term "arrangement", "installation", "link", "connection" should do broad sense understanding, for example, can be fixed connection, also can be detachable connection, or integrally connected;Can be mechanical connection, also can be electrical connection;Can be directly connected, also can be indirectly connected through the intermediate medium, can be two elements inside the communication.For ordinary skilled in the art, the above-mentioned term can be understood in the utility model according to the specific meaning of the specific circumstances.

[0026] Embodiment 1

[0027] As Figures 1-4 shown, the present embodiment provides a cryopreservation box with program cooling function, including box body 1, cover 2 and placing assembly, the cover 2 is hinged with the box body 1, the placing assembly is arranged in the box body 1 and is movably connected with the inner wall of the box body 1, the bottom surface of the placing assembly and the inner bottom surface of the box body 1 form a liquid storage cavity 9, the box body 1 is provided with liquid injection port 7 and liquid discharge port 8 communicated with the liquid storage cavity 9;The outer wall of the box body 1 is also provided with a cooling assembly, the cooling assembly includes condensing box 10, air suction pump 11, air suction pipe 12 and air return pipe 13, the air suction pump 11 is connected with the condensing box 10 and the air suction pipe 12 respectively, the other end of the air suction pipe 12 is communicated with the inside of the box body 1, and the two ends of the air return pipe 13 are respectively communicated with the condensing box 10 and the inside of the box body 1.

[0028] The liquid storage cavity 9 and the cooling assembly cooperate, not only can the cryopreserved cells be cooled, but also the cryopreserved cells after cooling can be directly cryopreserved, without transferring the cryopreserved cells, avoiding the influence of frequent taking on the biological activity of the cryopreserved cells, preventing the frostbite of the fingers of the staff. The liquid storage cavity 9 is provided with a refrigerant medium, such as liquid nitrogen, the liquid nitrogen is injected into the liquid storage cavity 9 by the liquid injection port 7, and the liquid nitrogen is discharged from the liquid storage cavity 9 by the liquid discharge port 8, so as to facilitate the recycling of the liquid nitrogen.

[0029] The placing assembly can place the cryopreservation tube 16 in the box body 1, and the cryopreservation tube 16 can be cooled and cryopreserved in the box body 1, and the height of the cryopreservation tube 16 can be adjusted by moving the placing assembly, so that the cryopreservation tube 16 is in the refrigerant medium in the liquid storage cavity 9, and the cryopreservation effect of the cells is ensured.

[0030] In one embodiment of the present embodiment, the liquid storage cavity 9 can also inject a cooling medium to cool the cryopreservation tube 16. At this time, the liquid storage cavity 9 is a standby cooling assembly of the condensing box 10, after the liquid storage cavity 9 completes cooling, the cooling medium is discharged, the refrigerant medium is injected, the switching of cooling and cryopreservation is realized, and the cryopreservation box has the dual functions of cooling and cryopreservation.

[0031] In the embodiment, the placing assembly comprises a placing rack 3 and a fixing plate 4, a plurality of through holes 15 are formed in the placing rack 3, a fixing groove 19 corresponding to each through hole 15 is arranged on the fixing plate 4, and the fixing plate 4 is detachably connected with the placing rack 3. Specifically, the fixing plate 4 and the placing rack 3 are clamped. The fixing plate 4 and the placing rack 3 cooperate to fix the cryopreservation tube 16, so that the cryopreservation tube 16 is prevented from shaking during transfer.

[0032] In the embodiment, a sliding groove 5 is formed in the inner wall of the box body 1, both ends of the placing rack 3 are installed in the sliding groove 5, an electric push rod 6 is arranged at the bottom of the sliding groove 5, and the push rod end of the electric push rod 6 is in contact with the bottom surface of the placing rack 3. After the freezing is completed, the electric push rod 6 can be used to lift the placing rack 3, so that the cryopreservation tube 16 is moved to the opening of the box body 1, and the cryopreservation tube 16 is convenient to take.

[0033] In the embodiment, the cryopreservation tube 16 is arranged in the through hole 15, and the tube cover of the cryopreservation tube 16 is clamped in the fixing groove 19. When the cryopreservation tube 16 is taken out, the fixing plate 4 can be directly taken out, so that all the cryopreservation tubes 16 can be taken out at one time, without the need to take out one by one, so that the effect of cell freezing is not affected by multiple times of taking out.

[0034] In the embodiment, the bottom of the cryopreservation tube 16 is provided with a heat preservation sleeve 17, a sandwich layer 18 is arranged between the inner and outer walls of the heat preservation sleeve 17, and a cold preservative is arranged in the sandwich layer 18. The cold preservative in the heat preservation sleeve 17 can still have a certain heat preservation effect on the cryopreservation tube 16 after being taken out, so that the temperature difference between the inside and outside of the freezing box is not large, and the activity of the cells in the cryopreservation tube 16 is not affected.

[0035] In the embodiment, the bottom of the condensing box 10 is provided with a refrigerating device 14. The refrigerating device 14 can continuously provide cold air to the box body 1, so that the box body 1 has good cooling effect.

[0036] In the embodiment, the box body 1 is provided with an observation window 20. The observation window 20 can observe the freezing medium in the liquid storage cavity 9, so that the freezing medium is not too much or too little.

[0037] The working principle of the freezing box with the programmed cooling function is as follows: the cells are placed in the cryopreservation tube 16, the cryopreservation tube 16 is sealed by the tube cover, the heat preservation sleeve 17 is sleeved on the bottom of the cryopreservation tube 16, the tube cover of the cryopreservation tube 16 is clamped in the fixing groove 19 of the fixing plate 4, the fixing plate 4 is placed on the placing rack 3 in the box body 1, and the cryopreservation tube 16 is arranged in the through hole 15, the air pump 11 and the refrigerating device 14 are started, the cold air enters the inside of the box body 1 through the air return pipe 13, the gas in the box body 1 enters the condensing box 10 through the air suction pipe 12, a cold air circulation is formed, and the cells are cooled.

[0038] After the temperature is reduced, the placing rack 3 is moved to the bottom of the sliding groove 5, the freezing medium is injected into the storage cavity 9 through the injection port 7, and the cells are subjected to cryopreservation treatment. After the cryopreservation is completed, the freezing medium is discharged from the discharge port 8, the electric push rod 6 is started, the placing rack 3 is raised, and the placing rack 3 is raised to the opening position of the box body 1. The fixed plate 4 is extracted, the cryopreservation tube 16 on the fixed plate 4 is taken off, and the cryopreservation tube 16 on the fixed plate 4 is taken off.

[0039] The preferred embodiments of the utility model are merely used for limiting the utility model, and for the technical personnel in the art, the utility model can have various changes and changes. Any modification, equivalent replacement, improvement and the like within the spirit and principle of the utility model should be included in the protection scope of the utility model.

Claims

1. A cryopreservation box with programmed cooling function, characterized in that: The application relates to a box body, a cover body and a placing assembly, the cover body is hinged to the box body, the placing assembly is arranged in the box body and movably connected with the inner wall of the box body, a liquid storage cavity is formed between the bottom surface of the placing assembly and the inner bottom surface of the box body, liquid injection ports and liquid discharge ports are arranged on the box body and communicate with the liquid storage cavity, a cooling assembly is further arranged on the outer wall of the box body, the cooling assembly comprises a condensing box, an air suction pump, an air suction pipe and an air return pipe, the air suction pump is connected with the condensing box and the air suction pipe, the other end of the air suction pipe communicates with the inside of the box body, and the two ends of the air return pipe respectively communicate with the condensing box and the inside of the box body.

2. The cryo-preservation box with a programmed temperature lowering function according to claim 1, wherein, The placing assembly comprises a placing rack and a fixing plate, a plurality of through holes are formed in the placing rack, the fixing plate is provided with fixing grooves corresponding to the through holes, and the fixing plate and the placing rack are detachably connected.

3. The cryo-preservation box with a programmed temperature lowering function according to claim 2, characterized in that, The inner wall of the box body is provided with a sliding groove, the two ends of the placing rack are installed in the sliding groove, the bottom of the sliding groove is provided with an electric push rod, and the push rod end of the electric push rod is in contact with the bottom surface of the placing rack.

4. The cryo-box with a programmed temperature lowering function according to claim 2, characterized in that, A cryopreservation tube is arranged in the through hole, and a tube cover of the cryopreservation tube is clamped in the fixing groove.

5. The cryo-box with a programmed temperature lowering function according to claim 4, characterized in that, The bottom of the cryopreservation tube is provided with a heat preservation sleeve, a sandwich layer is arranged between the inner wall and the outer wall of the heat preservation sleeve, and a cold preservative is arranged in the sandwich layer.

6. The cryo-storage box with a programmed temperature lowering function according to claim 1, characterized in that, The bottom of the condensing box is provided with a refrigerating device.

7. The cryo-storage box with a programmed temperature lowering function according to claim 1, characterized in that, The box body is provided with an observation window.

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