Efficient chromatographic column separation device

Abstract

The utility model provides an efficient chromatographic column separation device, and belongs to the technical field of chromatographic columns. The efficient chromatographic column separation device comprises a frame, a lifting mechanism is mounted on the frame, a sampling pipe is arranged at the top end of the frame, a screw is movably arranged in the sampling pipe, a blocking piece is rotatably connected to the lower end of the screw, the blocking piece movably blocks the lower end of the sampling pipe, a piston is in threaded connection with the screw, and the piston is in threaded connection with the lifting mechanism. And the piston is circumferentially fixed with the inner wall of the sampling tube. The device has the following effects that the opening and closing of the opening part of the sampling pipe can be realized by driving the axial movement of the screw rod through the switching mechanism, and the piston can axially move to generate a suction effect by utilizing the rotating mechanism to drive the screw rod to rotate and utilizing the circumferential fixing effect of the sampling pipe on the piston, so that the piston can be propped against the blocking piece when the device is immersed into chromatographic liquid; therefore, the gas is prevented from being brought into the chromatographic column, the disturbance to the chromatographic liquid is reduced, and the separation effect is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of chromatography column, in particular to a high-efficiency chromatography column separation device. BACKGROUND

[0002] Chromatography is the abbreviation of color layer analysis, which is a method for separating and measuring multi-component mixtures by using different physical properties of various components. It has been widely used in pharmaceutical, fine chemical and other fields. The current chromatography column separation equipment is static during the separation process, which leads to long separation time and low efficiency. In addition, the separation is carried out by discharging from the bottom of the column tube, so the chromatography liquid surface with specific requirements cannot be extracted separately.

[0003] To this end, Chinese patent application No. CN202021043114.1 discloses a high-efficiency column chromatography separation equipment. In this scheme, the sampling pipe is inserted into the column chromatography pipe to a certain height, the first air cylinder pushes the I-shaped ring cavity to extend the sampling pipe, and after the chromatography liquid surface enters the I-shaped ring cavity, the I-shaped ring cavity is pulled back to seal the chromatography liquid in the I-shaped ring cavity, which does not affect the rest of the chromatography liquid to continue, thereby improving the separation effect.

[0004] However, the present inventors found at least the following technical problems in the process of implementing the technical solutions in the embodiments of the present application:

[0005] There is air in the I-shaped ring cavity, which will be squeezed into the chromatography liquid to form bubbles after being opened in the chromatography liquid. The bubbles float out of the liquid surface from the layer where they are, which disrupts the original layered state. Therefore, the separation needs to be restarted after the layered state is stable again, which affects the separation efficiency. INVENTION CONTENTS

[0006] In order to make up for the above shortcomings, the present application provides a high-efficiency chromatography column separation device, which aims to improve the problems mentioned in the background art.

[0007] The present application provides a high-efficiency chromatography column separation device, which comprises a frame, a lifting mechanism mounted on the frame, a sampling pipe provided at the top of the frame, a screw rod movably arranged in the sampling pipe, a baffle plate rotatably connected to the lower end of the screw rod, the baffle plate movably sealing the lower end of the sampling pipe, a piston screwed on the screw rod, the piston being fixed to the inner wall of the sampling pipe in a circumferential direction, the piston movably abutting against the baffle plate, a switch mechanism provided in the sampling pipe to drive the screw rod to move axially, a rotating mechanism provided on the frame to drive the screw rod to rotate, and a chromatography column provided on the lifting mechanism.

[0008] In a specific embodiment, the lifting mechanism comprises a supporting plate, the chromatography column is inserted into the supporting plate, and the supporting plate is vertically and slidingly connected to the frame.

[0009] In the implementation process, the chromatographic column is inserted and taken out, which is convenient.

[0010] In a specific embodiment, the lifting mechanism further comprises a screw rod and a lifting motor, the screw rod is rotatably connected to the frame, the lifting motor is installed on the frame, the output end of the lifting motor is connected to the screw rod shaft, and the screw rod is threadedly connected to the supporting plate.

[0011] In the implementation process, the lifting motor is provided with a double head, which drives the two screw rods to rotate synchronously through the meshing of the gears, and then drives the supporting plate to vertically lift, thereby achieving the effect of inserting the sampling tube into the chromatographic column.

[0012] In a specific embodiment, the bottom of the frame is provided with a magnetic stirrer.

[0013] In the implementation process, the magnetic sub of the magnetic stirrer is placed in the chromatographic column. When stirring, the lifting mechanism lowers the chromatographic column, the sampling tube leaves the chromatographic column, and the lower end of the chromatographic column is attached to the magnetic stirrer. The magnetic field rotation drives the magnetic sub to rotate, thereby producing the stirring effect.

[0014] In a specific embodiment, the switch mechanism comprises a coil and a magnetic sheet, the magnetic sheet is rotatably connected to the screw rod, the coil is fixedly connected to the inner wall of the sampling tube, the screw rod penetrates the coil, and the magnetic sheet is magnetically connected to the coil.

[0015] In the implementation process, the coil is energized to push the magnetic sheet upward, and then the plug is tightly closed at the lower end of the sampling tube through the screw rod, thereby achieving the effect of closing. When the coil is reversely energized, the magnetic sheet is attracted downward, and then the plug is opened, and sampling can begin. It should be noted that the coil is energized in a slow lifting magnetic force mode, so that the plug can slowly open and close, reducing the disturbance to the chromatographic liquid.

[0016] In a specific embodiment, the piston comprises a nut and a sealing gasket, the nut is attached to the end face of the sealing gasket, and the nut is fixedly connected to the inner wall of the sampling tube.

[0017] In the implementation process, a limiting groove is formed in the outer circle of the nut, and a limiting strip is arranged axially on the inner wall of the sampling tube. In this way, the limiting groove is clamped on the limiting strip, so that the nut cannot rotate but can only move axially. Therefore, when the screw rod rotates, it can push the nut to move axially, thereby producing the effect of liquid suction. The sealing gasket is used here to improve the sealing performance.

[0018] In a specific embodiment, the rotating mechanism comprises a prism sleeve and a prism rod, the prism rod is fixedly connected to the upper end of the screw rod, the prism sleeve is rotatably connected to the frame, and the prism rod is inserted into the prism sleeve.

[0019] In one specific implementation, the rotating mechanism further includes a rotary motor and bevel gears, the rotary motor being mounted on the frame, and the output end of the rotary motor being connected to the prism shaft via a pair of bevel gears.

[0020] In the above implementation process, one of the two bevel gears is fixed to the output end of the rotary motor, and the other is fixed to the prism sleeve. In this way, the rotary motor can drive the prism sleeve to rotate, which in turn drives the screw to rotate. By sliding the prism sleeve axially, it is ensured that the screw will not affect the circumferential connection when it moves axially.

[0021] Compared with the prior art, the beneficial effects of this application are: the axial movement of the screw driven by the switching mechanism can realize the opening and closing of the sampling tube opening; the rotation mechanism drives the screw to rotate and the circumferential fixation effect of the sampling tube on the piston allows the piston to move axially to generate a suction effect. In this way, when the chromatography liquid is submerged, the piston can press against the plug, thereby avoiding the introduction of gas into the chromatography column, reducing disturbance to the chromatography liquid, and improving the separation effect. Attached Figure Description

[0022] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.

[0023] Figure 1 This is a first-view schematic diagram of the high-efficiency chromatography column separation device provided in the embodiments of this application;

[0024] Figure 2 A second-view schematic diagram of the high-efficiency chromatography column separation device provided for the embodiments of this application;

[0025] Figure 3 A first-view schematic diagram of the sampling tube cross-section provided for an embodiment of this application;

[0026] Figure 4 This is a second-view schematic diagram of the cross-section of the sampling tube provided for an embodiment of this application.

[0027] In the diagram: 10-Frame; 11-Magnetic stirrer; 20-Lifting mechanism; 21-Panel; 22-Screw; 23-Lifting motor; 30-Sampling tube; 40-Screw; 50-Piston; 51-Nut; 52-Sealing gasket; 60-Blocking plate; 70-Switching mechanism; 71-Coil; 72-Magnetic plate; 80-Rotation mechanism; 81-Rhombus sleeve; 82-Rhombus rod; 83-Rotation motor; 84-Bevel gear; 90-Chromatography column. Detailed Implementation

[0028] The technical solutions in the embodiments of this application will now be described with reference to the accompanying drawings.

[0029] Please see Figures 1-4 This application provides a high-efficiency chromatography column separation device, including a frame 10, a lifting mechanism 20 installed on the frame 10, a sampling tube 30 disposed at the top of the frame 10, a screw 40 movably disposed inside the sampling tube 30, a plug 60 rotatably connected to the lower end of the screw 40, the plug 60 movably sealing the lower end of the sampling tube 30, a piston 50 screwed onto the screw 40, the piston 50 being circumferentially fixed to the inner wall of the sampling tube 30, the piston 50 movably abutting against the plug 60, a switching mechanism 70 disposed inside the sampling tube 30 driving the screw 40 to move axially, a rotating mechanism 80 disposed on the frame 10 driving the screw 40 to rotate, and a chromatography column 90 disposed on the lifting mechanism 20. The switching mechanism 70 drives the axial movement of the screw 40 to open and close the sampling tube 30. The rotating mechanism 80 drives the screw 40 to rotate and uses the circumferential fixation of the sampling tube 30 on the piston 50, allowing the piston 50 to move axially and generate a suction effect. When the piston is submerged in the chromatography liquid, it can press against the plug 60, thereby preventing gas from being carried into the chromatography column 90, reducing disturbance to the chromatography liquid, and improving the separation effect.

[0030] Please see Figures 1-4 The lifting mechanism 20 includes a support plate 21, with the chromatography column 90 inserted into the support plate 21. The support plate 21 is vertically slidably connected to the frame 10. The chromatography column 90 is inserted for easy placement and removal.

[0031] Please see Figures 1-4 The lifting mechanism 20 also includes a lead screw 22 and a lifting motor 23. The lead screw 22 is rotatably connected to the frame 10, and the lifting motor 23 is mounted on the frame 10. The output end of the lifting motor 23 is connected to the shaft of the lead screw 22, and the lead screw 22 is threadedly connected to the support plate 21. The lifting motor 23 adopts a double-headed configuration, driving the two lead screws 22 to rotate synchronously through gear meshing, thereby driving the support plate 21 to lift vertically, achieving the effect of inserting the sampling tube 30 into the chromatography column 90.

[0032] Please see Figures 1-4 A magnetic stirrer 11 is provided at the bottom of the frame 10. The magnetic stirrer 11 is placed inside the chromatography column 90. When stirring, the lifting mechanism 20 lowers the chromatography column 90, the sampling tube 30 leaves the chromatography column 90, and the lower end of the chromatography column 90 is attached to the magnetic stirrer 11. The magnetic field rotation drives the magnetic stirrer to rotate, thereby producing a stirring effect.

[0033] Please see Figures 1-4The switching mechanism 70 includes a coil 71 and a magnetic sheet 72. The magnetic sheet 72 is rotatably connected to a screw 40, and the coil 71 is fixedly connected to the inner wall of the sampling tube 30. The screw 40 passes through the coil 71, and the magnetic sheet 72 is magnetically connected to the coil 71. When the coil 71 is energized, it pushes the magnetic sheet 72 upward, which in turn presses the plug 60 against the lower end of the sampling tube 30 through the screw 40, achieving a sealing effect. When the coil 71 is energized in the reverse direction, it pulls the magnetic sheet 72 downward, which in turn opens the plug 60, allowing sampling to begin. It should be noted that the coil 71 is energized by slowly increasing the magnetic force, so that the opening and closing of the plug 60 can be slow, reducing disturbance to the chromatography solvent.

[0034] Please see Figures 1-4 The piston 50 includes a nut 51 and a sealing gasket 52. The end faces of the nut 51 and the sealing gasket 52 are fitted together, and the nut 51 is circumferentially fixed to the inner wall of the sampling tube 30. A limiting groove is formed on the outer circle of the nut 51, and a limiting strip is axially provided on the inner wall of the sampling tube 30. By using the limiting groove to lock onto the limiting strip, the nut 51 cannot rotate but can only move axially. Therefore, when the screw 40 rotates, it can push the nut 51 to move axially, producing a liquid suction effect. The sealing gasket 52 is used here to improve the sealing performance.

[0035] Please see Figures 1-4 The rotating mechanism 80 includes a sleeve 81 and a rod 82. The rod 82 is fixedly connected to the upper end of the screw 40, and the sleeve 81 is rotatably connected to the frame 10. The rod 82 is inserted into the sleeve 81. The rotating mechanism 80 also includes a rotary motor 83 and bevel gears 84. The rotary motor 83 is mounted on the frame 10, and its output end is connected to the shaft of the sleeve 81 via a pair of bevel gears 84. One bevel gear 84 is fixed to the output end of the rotary motor 83, and the other is fixed to the sleeve 81. This allows the rotary motor 83 to drive the sleeve 81 to rotate, which in turn drives the screw 40 to rotate. The axial sliding of the rod 82 within the sleeve 81 ensures that the circumferential connection is not affected during the axial movement of the screw 40.

[0036] The working principle of this high-efficiency chromatography column separation device is as follows: The chromatography column 90 first adheres to the magnetic stirrer 11 for stirring. Then, the magnetic stirrer 11 stops, and the chromatography liquid begins to separate. The lifting motor 23 drives the lead screw 22 to rotate, thereby pushing the support plate 21 upward, allowing the sampling tube 30 to enter the chromatography column 90. When the lower end of the sampling tube 30 reaches the specified depth, the coil 71 attracts the magnetic sheet 72, pushing the screw 40 and the plug 60 downward, causing the plug 60 to leave the lower end of the sampling tube 30. Then, the rotary motor 83 drives the prism sleeve 81 to rotate, thereby driving the screw 40 to rotate. Since the piston 50 is circumferentially restricted, it begins to move axially. The piston 50 leaves the plug 60 and gradually retracts into the sampling tube 30, achieving the suction effect of the chromatography liquid. Then, coil 71 is energized in reverse, pushing magnetic plate 72 away, which in turn pushes screw 40 upward. This causes piston 50 and plug 60 to move upward, and plug 60 re-plugs the lower end of sampling tube 30, sealing the sample inside. Finally, chromatography column 90 moves downward, exposing sampling tube 30. In summary, the axial movement of screw 40 driven by switching mechanism 70 can open and close the opening of sampling tube 30. The rotation mechanism 80 drives screw 40 to rotate, and the circumferential fixation of piston 50 by sampling tube 30 allows piston 50 to move axially to generate a suction effect. When the sample is submerged in chromatography liquid, piston 50 can press against plug 60, thus preventing gas from being carried into chromatography column 90, reducing disturbance to chromatography liquid, and improving separation effect.

[0037] The above are merely embodiments of this application and are not intended to limit the scope of protection of this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, improvements, or equivalent substitutions made within the spirit and principles of this application should be included within the scope of protection of this application. It should be noted that similar reference numerals and letters in the following figures indicate similar items; therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

Claims

1. A high performance column separation device, characterized by, The utility model provides a chromatography device, including frame (10), the frame (10) on installation elevating system (20), the frame (10) top is provided with sampling pipe (30), the screw rod (40) is movably arranged in sampling pipe (30), the screw rod (40) lower end rotatory joint has the stop piece (60), the stop piece (60) is movably sealed in the sampling pipe (30) lower end, the screw rod (40) is screwed with piston (50), the piston (50) is fixed with the sampling pipe (30) inner wall circumference, the piston (50) is movably abutted in the stop piece (60), the sampling pipe (30) is provided with switch mechanism (70) drive screw rod (40) axial movement, the frame (10) is provided with rotating mechanism (80) drive screw rod (40) rotation, the elevating system (20) is provided with chromatography column (90).

2. The high performance column separation device of claim 1, wherein, The elevating system (20) includes a supporting plate (21), the chromatography column (90) is inserted with the supporting plate (21), the supporting plate (21) is vertically and slidingly connected with the frame (10).

3. The high performance column separation device of claim 2, wherein, The elevating system (20) further includes a lead screw (22) and a lifting motor (23), the lead screw (22) is rotatably connected with the frame (10), the lifting motor (23) is installed on the frame (10), an output end of the lifting motor (23) is connected with the lead screw (22), and the lead screw (22) is threadedly connected with the supporting plate (21).

4. The high performance column separation device of claim 3, wherein, The frame (10) is provided with a magnetic stirrer (11) at the bottom.

5. The high performance column separation device of claim 4, wherein, The switch mechanism (70) includes a coil (71) and a magnetic sheet (72), the magnetic sheet (72) is rotatably connected with the screw rod (40), the coil (71) is fixedly connected to the inner wall of the sampling pipe (30), the screw rod (40) penetrates the coil (71), and the magnetic sheet (72) is magnetically connected with the coil (71).

6. The high performance column separation device of claim 5, wherein, The piston (50) includes a nut (51) and a sealing gasket (52), the nut (51) is attached to the end face of the sealing gasket (52), and the nut (51) is fixedly connected to the inner wall of the sampling pipe (30).

7. A high performance column separation device according to claim 6, wherein, The rotating mechanism (80) includes a prism sleeve (81) and a prism rod (82), the prism rod (82) is fixedly connected with the upper end of the screw rod (40), the prism sleeve (81) is rotatably connected with the frame (10), and the prism rod (82) is inserted with the prism sleeve (81).

8. The high performance column separation device of claim 7, wherein, The rotating mechanism (80) further includes a rotating motor (83) and bevel gears (84), the rotating motor (83) is installed on the frame (10), and an output end of the rotating motor (83) is connected with the prism sleeve (81) through the bevel gears (84).

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