Device for cleaning, disinfecting and removing nucleic acid from experimental apparatus

Abstract

The utility model discloses a device for cleaning, disinfecting and removing nucleic acid from experimental instruments. The device comprises a case, and a spraying system, an ultraviolet disinfection system, a drying system and a control display system which are arranged in the case. The cleaning, disinfecting and nucleic acid removing device ensures the cleaning, disinfecting and nucleic acid removing effects, does not have chemical reagent residues, and realizes cleaning, disinfecting and nucleic acid removing of experimental apparatuses such as pipettes.

Description

Technical Field

[0001] This utility model relates to the fields of biosafety and biomedicine, and more specifically to a device for cleaning, disinfecting and removing nucleic acid from experimental equipment. Background Technology

[0002] Currently, biosafety and biomedical laboratories widely use laboratory equipment such as pipettes. Due to contact with pathogenic microorganisms or nucleic acid samples, autoclaving is time-consuming, labor-intensive, and extremely inconvenient. Most laboratories do not handle these samples promptly, posing a risk of cross-contamination by microorganisms and nucleic acids.

[0003] Therefore, how to efficiently clean, disinfect, and remove nucleic acid from experimental equipment is a problem that urgently needs to be solved by those skilled in the art. Utility Model Content

[0004] In view of this, the purpose of this utility model is to provide a device for cleaning, disinfecting, and removing nucleic acid from experimental instruments, thereby overcoming the shortcomings of the prior art. This utility model ensures the effectiveness of cleaning, disinfecting, and removing nucleic acid without leaving any chemical reagent residues, thus realizing the cleaning, disinfection, and nucleic acid removal of experimental instruments such as pipettes.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A device for cleaning, disinfecting and removing nucleic acid from experimental equipment includes a chassis, and a spray system, an ultraviolet disinfection system, a drying system and a control and display system placed inside the chassis;

[0007] The chassis is a square cavity, which includes, from bottom to top, a component cavity, a disinfection cavity, and a sealing cover connected in sequence. The top of the disinfection cavity is sealed by the sealing cover. The sealing cover is connected to the outer shell of the chassis by a damping pivot and can be fixed at any angle from 0° to 190°. A sealing ring is embedded inside and magnetically attached to the outer shell of the chassis.

[0008] The spray system includes a bracket, a Venturi nozzle, an infrared sensor, pipes, a 2-position 2-way solenoid valve, a peristaltic pump, a 2-position 3-way valve, a disinfection and nucleic acid removal reagent bottle, a cleaning reagent bottle, disinfection and nucleic acid removal reagent, cleaning reagent, a level sensor for the disinfection and nucleic acid removal reagent bottle, a level sensor for the cleaning reagent bottle, and an air pump. The bracket, Venturi nozzle, and infrared sensor are placed in the disinfection chamber. The pipes, 2-position 2-way solenoid valve, peristaltic pump, 2-position 3-way valve, disinfection and nucleic acid removal reagent bottle, cleaning reagent bottle, disinfection and nucleic acid removal reagent, cleaning reagent, level sensor for the disinfection and nucleic acid removal reagent bottle, level sensor for the cleaning reagent bottle, and air pump are placed in the component chamber. The Venturi nozzle, 2-position 2-way solenoid valve, peristaltic pump, 2-position 3-way valve, disinfection and nucleic acid removal reagent bottle, cleaning reagent bottle, and air pump are connected by pipes. The disinfection and nucleic acid removal reagent is placed in the disinfection and nucleic acid removal reagent bottle, and the cleaning reagent is placed in the cleaning reagent bottle.

[0009] The ultraviolet disinfection system includes ultraviolet lamps and ultraviolet intensity sensors, both of which are placed in the disinfection chamber;

[0010] The drying system includes a PTC heating element, a drying fan, a semiconductor refrigeration module, a cooling fan, a water pump, a water tank, an exhaust fan, an exhaust port, a vacuum pump, a one-way valve, a waste liquid bottle, a waste liquid bottle level sensor, and a temperature and humidity sensor. There are two semiconductor refrigeration modules. The PTC heating element, the drying fan, one of the semiconductor refrigeration modules, the exhaust fan, the exhaust port, and the temperature and humidity sensor are placed in the disinfection chamber. The other semiconductor refrigeration module, the cooling fan, the water pump, the water tank, the vacuum pump, the one-way valve, the waste liquid bottle, and the waste liquid bottle level sensor are placed in the component chamber. The semiconductor refrigeration module, the water pump, the water tank, the exhaust port, the vacuum pump, the one-way valve, and the waste liquid bottle are connected by pipes.

[0011] The control and display system includes a control circuit and a touch screen. The control circuit is connected to an infrared sensor, a two-position two-way solenoid valve, a peristaltic pump, a two-position three-way valve, a liquid level sensor for disinfection and nucleic acid removal reagent bottles, a liquid level sensor for cleaning reagent bottles, a liquid level sensor for waste liquid bottles, an air pump, an ultraviolet lamp, an ultraviolet intensity sensor, a PTC heating element, a drying fan, a semiconductor refrigeration module, a cooling fan, a water pump, an exhaust fan, a vacuum pump, a temperature and humidity sensor, and the touch screen to control its operation.

[0012] Furthermore, the aforementioned support is equipped with 1-10 hooks and grooved positions for placing experimental instruments, which are tilted at 10°-90°.

[0013] The further beneficial effect of adopting the above is that the support is a machined part used to place experimental instruments, which can hold 1-10 experimental instruments including pipettes at the same time; the tilted placement makes it easier for residual reagents to be discharged to the waste bottle by the vacuum pump.

[0014] Furthermore, the aforementioned Venturi nozzle consists of a conical drill bit with a circular cross-section, a cylindrical throat, and a conical outlet. It is installed at the bottom of the support, with the number corresponding to the number of experimental apparatus placement positions on the support, forming a one-to-one correspondence. The Venturi nozzle is equipped with anti-clogging spray holes aligned with the inner cavity of the experimental apparatus. The Venturi nozzles are sequentially connected to an air pump and a sterilization chamber via pipes, forming a gas circuit with the sterilization chamber. The Venturi nozzles are sequentially connected to a two-position two-way solenoid valve, a peristaltic pump, a two-position three-way valve, a sterilization and nucleic acid removal reagent bottle, and a cleaning reagent bottle via pipes. The sterilization and nucleic acid removal reagents and cleaning reagents are sprayed through the spray holes in micron-sized particles onto the surface and inner cavity of the experimental apparatus, achieving the purpose of cleaning, disinfection, and nucleic acid removal.

[0015] Furthermore, the aforementioned infrared sensors are installed at the bottom of the support, and their number corresponds to the number of experimental equipment placement positions on the support, forming a one-to-one relationship.

[0016] The further beneficial effect of using the above-mentioned infrared sensors is that they are used to monitor the location and quantity of experimental equipment.

[0017] Furthermore, the aforementioned pipe is a common component, typically made of silicone tubing.

[0018] Furthermore, the aforementioned two-position two-way solenoid valve is an outsourced component. It is a normally closed solenoid valve, and its function is to open the valve when energized, so that disinfection and nucleic acid removal reagents or cleaning reagents can be sprayed through the pipeline for disinfection or cleaning.

[0019] Furthermore, the aforementioned peristaltic pump is an outsourced component, its function being to circulate disinfectant or cleaning reagents within the pipeline without contacting the liquid.

[0020] Furthermore, the aforementioned two-position three-way valve is an outsourced component, used to switch pipelines between disinfection and nucleic acid removal reagents and cleaning reagents.

[0021] Furthermore, the aforementioned disinfection and nucleic acid removal reagent bottles and cleaning reagent bottles are blow-molded parts with a volume of 100-2200mL. They include a bottle body, a sealing cap, a liquid outlet tube, and a gas inlet tube. The bottle body is provided with a grooved handle. The sealing cap consists of a threaded cap and a sealing disc. The liquid outlet tube and the liquid inlet tube are distributed on the sealing disc. The sealing cap and the bottle body are tightly fitted together.

[0022] Furthermore, the aforementioned disinfectant and nucleic acid removal reagent is a hydrogen peroxide solution containing a surfactant; the mass concentration of the hydrogen peroxide solution is 1.5%-6%; the surfactant is sodium dodecyl sulfate (SDS) with a mass concentration of 0.01%-0.1%, dissolved and diluted with a buffer solution with a pH of 7.0-8.0; the disinfectant and nucleic acid removal reagent is pre-filled in a disinfectant and nucleic acid removal reagent bottle with a volume of 100-2200mL, which is connected to two two-position three-way valves and a peristaltic pump in sequence through a pipeline, and then flows back to the disinfectant and nucleic acid removal reagent bottle to form a loop; on the loop, a three-way valve is connected in sequence through a pipeline to a two-position three-way valve and a Venturi nozzle, and disinfection is achieved through siphon effect spraying.

[0023] The further beneficial effect of using the above methods is that hydrogen peroxide solution, as a DNA / RNA disruptor, effectively removes nucleic acids, including amplified fragments, plasmids, genomic DNA, and RNA. Sodium dodecyl sulfate is used to wet, emulsify, disperse, degrease, and clean contaminants from experimental equipment.

[0024] Furthermore, the cleaning reagent is sterile purified water or water for injection; the cleaning reagent is pre-filled in a cleaning reagent bottle with a volume of 100-2200mL, and is connected in sequence to two two-position three-way valves and a peristaltic pump through a pipeline, and then flows back to the cleaning reagent bottle to form a loop; in the loop, a three-way valve is connected in sequence to a two-position three-way valve and a Venturi nozzle through a pipeline, and the cleaning is carried out by spraying through the siphon effect.

[0025] Furthermore, the aforementioned liquid level sensor is an outsourced component, used to monitor the liquid volume in disinfection and nucleic acid removal reagent bottles, cleaning reagent bottles, and waste liquid bottles.

[0026] Furthermore, the aforementioned air pump is an externally purchased component. Its function is to pump the disinfection and nucleic acid removal reagents and cleaning reagents through pipelines to the Venturi nozzle, where they are sprayed in micron-sized particles onto the pipettes and other experimental instruments placed in the disinfection chamber, thereby achieving the purpose of disinfection and nucleic acid removal.

[0027] Furthermore, the aforementioned ultraviolet lamps are UVC deep ultraviolet waterproof sterilization and disinfection modules, installed at the bottom of the bracket, with the number matching the number of experimental instrument placement positions on the bracket, forming a one-to-one correspondence.

[0028] The further beneficial effect of employing the above methods is that the ultraviolet lamp is used to disinfect experimental instruments such as pipettes placed in the sterilization chamber. When cleaning, disinfecting, and removing nucleic acid from experimental instruments, hydrogen peroxide solution is used in conjunction with ultraviolet light to enhance the disinfection effect. For cases of severe contamination, multiple cycles of disinfection are implemented.

[0029] Furthermore, the aforementioned ultraviolet intensity sensor is used to monitor ultraviolet intensity, automatically calculate the irradiation time based on the set ultraviolet irradiation dose to ensure disinfection effect, and promptly prompt for replacement when the ultraviolet lamp intensity weakens.

[0030] Furthermore, the aforementioned PTC heating element is a commonly used component, its function being to heat and automatically control the temperature to dry experimental instruments such as pipettes placed in the sterilization chamber.

[0031] Furthermore, the aforementioned drying fan is a commonly used component, its function being to transfer the heat generated by the PTC heating element to the sterilization chamber, thereby drying the experimental instruments such as pipettes placed inside the sterilization chamber.

[0032] Furthermore, the aforementioned semiconductor cooling module is composed of a heat sink, a semiconductor cooling chip, and a water cooling chip that are sequentially bonded together, with thermally conductive adhesive applied between them; the cold water generated by the semiconductor cooling module installed in the component cavity circulates through pipes through the water cooling chip of the semiconductor cooling module installed in the disinfection cavity.

[0033] The further beneficial effect of adopting the above-mentioned method is that the semiconductor cooling module plays a role in heat dissipation, while ensuring that the air is isolated from the outside world and the sterilization chamber is sealed.

[0034] Furthermore, the aforementioned cooling fan is a common component used to dissipate heat generated by the semiconductor cooling module.

[0035] Furthermore, the aforementioned water pump is a common component. Its function is to circulate the cold water generated by the semiconductor cooling module installed in the component cavity through pipes to the water-cooling plate of the semiconductor cooling module installed in the disinfection cavity, thereby achieving the function of heat dissipation.

[0036] Furthermore, the aforementioned water storage tank is a common component, its function being to store water.

[0037] Furthermore, the aforementioned exhaust fan is a commonly used component that blows the mist inside the disinfection chamber out through the exhaust port, thereby accelerating the drying process.

[0038] Furthermore, the aforementioned air pump is a commonly used component, its function being to discharge air mist, residual reagents, and condensed water droplets into the waste liquid bottle.

[0039] Furthermore, the aforementioned one-way valve is a commonly used component, its function being to ensure that mist and liquid can only flow from the disinfection chamber to the waste liquid bottle, and cannot flow in the opposite direction.

[0040] Furthermore, the aforementioned waste liquid bottle is a common component, used to store air mist, residual reagents, and condensed water droplets.

[0041] Furthermore, the aforementioned temperature and humidity sensor is a commonly used component, its function being to monitor the temperature and humidity inside the disinfection chamber and automatically determine the drying time based on the set endpoint temperature and humidity.

[0042] Furthermore, the control circuit described above calculates the spray volume based on the spray rate and time of the venturi nozzle, calculates the remaining volume in conjunction with the pre-filled amounts of the disinfection and nucleic acid removal reagents and the cleaning reagents, and displays the remaining volume on the touch screen, promptly prompting the user to replace the disinfection and nucleic acid removal reagents and the cleaning reagents.

[0043] Furthermore, the aforementioned touchscreen is a commonly used component, its function being to display and set operating parameters and status.

[0044] Furthermore, the aforementioned chassis is a square cavity in which the various components described above are installed.

[0045] A method for cleaning, disinfecting, and removing nucleic acid from laboratory equipment, using the aforementioned apparatus for cleaning, disinfecting, and removing nucleic acid from laboratory equipment, specifically includes the following steps:

[0046] (1) Connect the disinfection and nucleic acid removal reagent bottle and the cleaning reagent bottle to the tubing;

[0047] (2) Place the experimental apparatus on the stand;

[0048] (3) Power-on self-test: including the functional status of each electronic component;

[0049] (4) Setting parameters: Set the cleaning, disinfection, nucleic acid removal and drying parameters on the touch screen, including reagent volume, spray time, ultraviolet irradiation dose, endpoint temperature and endpoint humidity, and start the operation;

[0050] (5) Chemical reagent disinfection and cleaning: Infrared sensors monitor the position and quantity of experimental equipment. Disinfection and nucleic acid removal reagents and cleaning reagents are sprayed sequentially through Venturi nozzles in micron-level factors onto the surface and inner cavity of the experimental equipment. The reagent volume and spraying time are automatically determined according to the set cleaning, disinfection and nucleic acid removal parameters.

[0051] (6) Ultraviolet disinfection: Turn on the ultraviolet lamp and use ultraviolet disinfection in combination. The irradiation time is automatically determined according to the set ultraviolet irradiation dose and the monitored ultraviolet intensity.

[0052] (7) Automatic drying: When the drying system is turned on, the drying fan transfers the heat generated by the PTC heating element to the sterilization chamber. The hot air is blown to the surface and inner cavity of the pipette and other experimental instruments through the Venturi nozzle. The semiconductor cooling module condenses the mist into water droplets. The exhaust fan blows the mist inside the sterilization chamber out through the exhaust port. The air mist, residual reagents and condensed water droplets are discharged to the waste liquid bottle by the vacuum pump. The drying end time is automatically determined according to the set endpoint temperature and humidity and by monitoring the temperature and humidity.

[0053] (8) End the operation.

[0054] As can be seen from the above technical solution, compared with the prior art, the beneficial effects of this utility model are as follows:

[0055] 1. It integrates chemical reagent disinfection and cleaning with ultraviolet combined disinfection mode, achieving multiple purposes of cleaning, disinfection and nucleic acid removal for laboratory instruments such as pipettes;

[0056] 2. The Venturi nozzle spray hole is aligned with the inner cavity of the experimental apparatus. The disinfection and nucleic acid removal reagents and cleaning reagents are sprayed into the surface and inner cavity of the experimental apparatus in micron-sized particles through this hole. The quantity is consistent with the number of experimental apparatuses, forming a one-to-one correspondence, which ensures the effectiveness of cleaning, disinfection and nucleic acid removal.

[0057] 3. Infrared sensors, ultraviolet intensity sensors, and temperature and humidity sensors monitor the location and quantity of experimental equipment, ultraviolet intensity, and temperature and humidity, respectively, and automatically determine the number of Venturi nozzles to be turned on, the ultraviolet lamp irradiation time, and the drying end time, thus realizing automation and intelligence.

[0058] 4. By minimizing the use of chemical reagents and thoroughly cleaning away any residues, the cleaning and disinfection of experimental equipment such as pipettes removes nucleic acids, thus preventing cross-contamination by microorganisms and nucleic acids. Attached Figure Description

[0059] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0060] Figure 1 This is a schematic diagram of the structure of the nucleic acid removal device for cleaning, disinfecting and cleaning experimental equipment in Examples 1-2;

[0061] Figure 2 This is a schematic diagram of the multi-spray system of the nucleic acid removal device for cleaning, disinfecting and cleaning experimental equipment in Example 2;

[0062] Among them, 1-stand, 2-Venturi nozzle, 3-infrared sensor, 4-pipeline, 5-2-position 2-way solenoid valve, 6-peristaltic pump, 7-2-position 3-way valve, 8-sterilization and nucleic acid removal reagent bottle, 9-cleaning reagent bottle, 10-sterilization and nucleic acid removal reagent, 11-cleaning reagent, 121-sterilization and nucleic acid removal reagent bottle level sensor, 122-cleaning reagent bottle level sensor, 123-waste liquid bottle level sensor, 13-air pump, 14-ultraviolet lamp, 1 5-UV intensity sensor, 16-PTC heating element, 17-drying fan, 18-semiconductor refrigeration module, 19-cooling fan, 20-water pump, 21-water storage tank, 22-exhaust fan, 23-vent, 24-vacuum pump, 25-one-way valve, 26-waste liquid bottle, 27-temperature and humidity sensor, 28-control circuit, 29-touch screen, 30-chassis, 301-disinfection chamber, 302-component chamber, 303-sealed cover. Detailed Implementation

[0063] The embodiments of this utility model are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain this utility model, and should not be construed as limiting this utility model.

[0064] In the description of this utility model, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0065] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0066] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0067] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0068] Example 1

[0069] Devices for cleaning, disinfecting, and removing nucleic acid from experimental equipment, such as Figure 1 As shown, it includes a chassis 30, and a spray system, an ultraviolet disinfection system, a drying system and a control and display system placed inside the chassis 30;

[0070] The chassis 30 is a square cavity, which includes, from bottom to top, a component cavity 302, a disinfection cavity 301, and a sealing cover 303 connected in sequence. The top of the disinfection cavity 301 is sealed by the sealing cover 303. The sealing cover 303 is connected to the outer shell of the chassis 30 by a damping pivot and is fixed at 0°. A sealing ring is embedded inside and magnetically attached to the outer shell of the chassis 30.

[0071] The spray system includes a bracket 1, a Venturi nozzle 2, an infrared sensor 3, a pipe 4, a 2-position 2-way solenoid valve 5, a peristaltic pump 6, a 2-position 3-way valve 7, a disinfection and nucleic acid removal reagent bottle 8, a cleaning reagent bottle 9, a disinfection and nucleic acid removal reagent 10, a cleaning reagent 11, a disinfection and nucleic acid removal reagent bottle level sensor 121, a cleaning reagent bottle level sensor 122, and an air pump 13. The bracket 1, Venturi nozzle 2, and infrared sensor 3 are placed in the disinfection chamber 301. The pipe 4, 2-position 2-way solenoid valve 5, peristaltic pump 6, 2-position 3-way valve 7, and disinfection and nucleic acid removal reagent bottle are also included. Nucleic acid removal reagent bottle 8, cleaning reagent bottle 9, disinfectant nucleic acid removal reagent 10, cleaning reagent 11, disinfectant nucleic acid removal reagent bottle level sensor 121, cleaning reagent bottle level sensor 122, and air pump 13 are placed in component cavity 302. Venturi nozzle 2, two-position two-way solenoid valve 5, peristaltic pump 6, two-position three-way valve 7, disinfectant nucleic acid removal reagent bottle 8, cleaning reagent bottle 9, and air pump 13 are connected by pipes 4 respectively. Disinfectant nucleic acid removal reagent 10 is placed in disinfectant nucleic acid removal reagent bottle 8, and cleaning reagent 11 is placed in cleaning reagent bottle 9.

[0072] Among them, the support 1 has a groove structure for placing experimental equipment, which is tilted at 30°;

[0073] The Venturi nozzle 2 consists of a conical drill bit with a circular cross-section, a cylindrical throat, and a conical outlet. It is installed at the bottom of the support 1, and the number of nozzles corresponds to the number of experimental apparatus placement positions on the support 1, forming a one-to-one correspondence. The Venturi nozzle 2 is equipped with anti-clogging spray holes, which are aligned with the inner cavity of the experimental apparatus. The Venturi nozzle 2 is connected to the air pump 13 and the sterilization chamber 301 in sequence through pipes 4, forming a gas circuit with the sterilization chamber 301. The Venturi nozzle 2 is connected to the liquid circuit of the two-position two-way solenoid valve 5, the peristaltic pump 6, the two-position three-way valve 7, the sterilization and nucleic acid removal reagent bottle 8, and the cleaning reagent bottle 9 in sequence through pipes 4. The sterilization and nucleic acid removal reagent 10 and the cleaning reagent 11 are sprayed into the surface and inner cavity of the experimental apparatus in micron-level particles through the spray holes.

[0074] Infrared sensors 3 are installed below the support 1, and their number is consistent with the number of experimental equipment placement positions on the support 1, forming a one-to-one correspondence.

[0075] The disinfection and nucleic acid removal reagent bottle 8 and the cleaning reagent bottle 9 are blow-molded parts with a volume of 500mL. They include a bottle body, a sealing cap, a liquid outlet tube, and a liquid inlet tube. The bottle body is provided with a grooved handle. The sealing cap consists of a threaded cap and a sealing disc. The liquid outlet tube and the liquid inlet tube are distributed on the sealing disc. The sealing cap and the bottle body are tightly fitted together.

[0076] The disinfectant and nucleic acid removal reagent 10 is a hydrogen peroxide solution containing a surfactant; the mass concentration of the hydrogen peroxide solution is 4.0%; the surfactant is sodium dodecyl sulfate with a mass concentration of 0.05%, which is dissolved and diluted with a buffer solution with a pH of 7.0; the disinfectant and nucleic acid removal reagent 10 is pre-filled in the disinfectant and nucleic acid removal reagent bottle 8 with a volume of 300mL, and is connected to two two-position three-way valves 7 and a peristaltic pump 6 in sequence through pipe 4, and then flows back into the disinfectant and nucleic acid removal reagent bottle 8 to form a loop; in the loop, a three-way valve is connected to the two-position three-way valve 7 and the Venturi nozzle 2 in sequence through pipe 4, and disinfection is achieved by spraying through the siphon effect;

[0077] Cleaning reagent 11 is sterile purified water; cleaning reagent 11 is pre-filled in cleaning reagent bottle 9 with a volume of 300mL, and is connected in sequence to two two-position three-way valves 7 and peristaltic pump 6 through pipe 4, and then flows back to cleaning reagent bottle 9 to form a loop; in the loop, a three-way valve is connected in sequence to two-position three-way valve 7 and Venturi nozzle 2 through pipe 4, and cleaning is carried out by spraying through the siphon effect;

[0078] The ultraviolet disinfection system includes ultraviolet lamps 14 and ultraviolet intensity sensors 15, both of which are placed in the disinfection chamber 301. The ultraviolet lamps 14 are UVC deep ultraviolet waterproof sterilization modules with an ultraviolet intensity of 30000μW. They are installed at the bottom of the support 1, and their number corresponds to the number of experimental equipment placement positions on the support 1, forming a one-to-one correspondence. The ultraviolet intensity sensors 15 are used to monitor the ultraviolet intensity, automatically calculate the irradiation time according to the set ultraviolet irradiation dose, and promptly prompt for replacement when the ultraviolet lamp intensity decreases.

[0079] The drying system includes a PTC heating element 16, a drying fan 17, a semiconductor refrigeration module 18, a cooling fan 19, a water pump 20, a water storage tank 21, an exhaust fan 22, an exhaust port 23, a vacuum pump 24, a one-way valve 25, a waste liquid bottle 26, a waste liquid bottle level sensor 123, and a temperature and humidity sensor 27. There are two semiconductor refrigeration modules 18. One of the PTC heating element 16, the drying fan 17, the semiconductor refrigeration module 18, the exhaust fan 22, the exhaust port 23, and the temperature and humidity sensor 27 are placed in the sterilization chamber 301. The other semiconductor refrigeration module 18 includes a cooling fan 19 and a water pump 20. A water storage tank 21, an air pump 24, a one-way valve 25, a waste liquid bottle 26, and a waste liquid bottle level sensor 123 are placed in the component cavity 302. The semiconductor cooling module 18, the water pump 20, the water storage tank 21, the vent 23, the air pump 24, the one-way valve 25, and the waste liquid bottle 26 are connected by pipes 4. The semiconductor cooling module 18 is composed of a heat sink, a semiconductor cooling plate, and a water cooling plate that are sequentially bonded together and coated with thermally conductive adhesive. The cold water generated by the semiconductor cooling module 18 installed in the component cavity 302 circulates through the water cooling plate of the semiconductor cooling module 18 installed in the disinfection cavity 301 via pipes 4.

[0080] The control and display system includes a control circuit 28 and a touch screen 29. The control circuit 28 is connected to an infrared sensor 3, a two-position two-way solenoid valve 5, a peristaltic pump 6, a two-position three-way valve 7, a liquid level sensor 121 for disinfection and nucleic acid removal reagent bottles, a liquid level sensor 122 for cleaning reagent bottles, a liquid level sensor 123 for waste liquid bottles, an air pump 13, an ultraviolet lamp 14, an ultraviolet intensity sensor 15, a PTC heating element 16, a drying fan 17, a semiconductor refrigeration module 18, a cooling fan 19, a water pump 20, an exhaust fan 22, a vacuum pump 24, a temperature and humidity sensor 27, and a touch screen 29 via wiring.

[0081] Example 2

[0082] Devices for cleaning, disinfecting, and removing nucleic acid from experimental equipment, such as Figure 1 As shown, it includes a chassis 30, and a spray system, an ultraviolet disinfection system, a drying system and a control and display system placed inside the chassis 30;

[0083] The chassis 30 is a square cavity, which includes, from bottom to top, a component cavity 302, a disinfection cavity 301, and a sealing cover 303 connected in sequence. The top of the disinfection cavity 301 is sealed by the sealing cover 303. The sealing cover 303 is connected to the outer shell of the chassis 30 by a damping pivot and is fixed at 0°. A sealing ring is embedded inside and magnetically attached to the outer shell of the chassis 30.

[0084] like Figure 1 , Figure 2 As shown, the spray system includes a bracket 1, a Venturi nozzle 2, an infrared sensor 3, a pipe 4, a two-position two-way solenoid valve 5, a peristaltic pump 6, a two-position three-way valve 7, a disinfection and nucleic acid removal reagent bottle 8, a cleaning reagent bottle 9, a disinfection and nucleic acid removal reagent 10, a cleaning reagent 11, a disinfection and nucleic acid removal reagent bottle level sensor 121, a cleaning reagent bottle level sensor 122, and an air pump 13. The bracket 1, Venturi nozzle 2, and infrared sensor 3 are placed in the disinfection chamber 301. The pipe 4, two-position two-way solenoid valve 5, peristaltic pump 6, and two-position three-way valve 7... The disinfection and nucleic acid removal reagent bottle 8, cleaning reagent bottle 9, disinfection and nucleic acid removal reagent 10, cleaning reagent 11, disinfection and nucleic acid removal reagent bottle level sensor 121, cleaning reagent bottle level sensor 122, and air pump 13 are placed in component cavity 302. Venturi nozzle 2, two-position two-way solenoid valve 5, peristaltic pump 6, two-position three-way valve 7, disinfection and nucleic acid removal reagent bottle 8, cleaning reagent bottle 9, and air pump 13 are connected by pipes 4 respectively. Disinfection and nucleic acid removal reagent 10 is placed in disinfection and nucleic acid removal reagent bottle 8, and cleaning reagent 11 is placed in cleaning reagent bottle 9.

[0085] Among them, the support 1 has 5 grooved experimental apparatus placement positions, which are tilted at 45°;

[0086] The Venturi nozzle 2 consists of a conical drill bit with a circular cross-section, a cylindrical throat, and a conical outlet. It is installed at the bottom of the support 1, and the number of nozzles is consistent with the number of experimental apparatus placement positions on the support 1, which is 5, forming a one-to-one correspondence. The Venturi nozzle 2 is equipped with anti-clogging spray holes, which are aligned with the inner cavity of the experimental apparatus. The Venturi nozzle 2 is connected to 5 two-position two-way solenoid valves 5 in sequence through pipes 4, and then connected in parallel to the flow circuit of the disinfection and nucleic acid removal reagent 10 and the cleaning reagent 11. The disinfection and nucleic acid removal reagent 10 and the cleaning reagent 11 are sprayed into the surface and inner cavity of the experimental apparatus through the spray holes in the form of micron-sized particles.

[0087] Infrared sensors 3 are installed below the bracket 1, and the number of them is the same as the number of experimental equipment placement positions on the bracket 1, which is 5, forming a one-to-one correspondence.

[0088] The disinfection and nucleic acid removal reagent bottle 8 and the cleaning reagent bottle 9 are blow-molded parts with a volume of 500mL. They include a bottle body, a sealing cap, a liquid outlet tube, and a liquid inlet tube. The bottle body is provided with a grooved handle. The sealing cap consists of a threaded cap and a sealing disc. The liquid outlet tube and the liquid inlet tube are distributed on the sealing disc. The sealing cap and the bottle body are tightly fitted together.

[0089] The disinfectant and nucleic acid removal reagent 10 is a hydrogen peroxide solution containing a surfactant; the mass concentration of the hydrogen peroxide solution is 4.0%; the surfactant is sodium dodecyl sulfate with a mass concentration of 0.05%, dissolved and diluted with a buffer solution with a pH of 7.5; the disinfectant and nucleic acid removal reagent 10 is pre-filled in the disinfectant and nucleic acid removal reagent bottle 8 with a volume of 300mL, and is connected to two two-position three-way valves 7 and a peristaltic pump 6 in sequence through pipe 4, and then flows back into the disinfectant and nucleic acid removal reagent bottle 8 to form a loop; in the loop, a three-way valve is connected to the two-position three-way valve 7 and the Venturi nozzle 2 in sequence through pipe 4, and disinfection is achieved by spraying through the siphon effect;

[0090] Cleaning reagent 11 is sterile water for injection; cleaning reagent 11 is pre-filled in cleaning reagent bottle 9 with a volume of 300mL, and is connected in sequence to two two-position three-way valves 7 and peristaltic pump 6 through pipe 4, and then flows back to cleaning reagent bottle 9 to form a loop; in the loop, a three-way valve is connected in sequence to two-position three-way valve 7 and Venturi nozzle 2 through pipe 4, and cleaning is carried out by spraying through the siphon effect;

[0091] Air pump 13 is connected to Venturi nozzle 2 through pipes, and the number of nozzles is the same as the number of Venturi nozzles 2, which is 5, in a one-to-one correspondence, and then connected in parallel to disinfection chamber 301;

[0092] The ultraviolet disinfection system includes ultraviolet lamps 14 and ultraviolet intensity sensors 15, both placed in the disinfection chamber 301. The ultraviolet lamps 14 are UVC deep ultraviolet waterproof sterilization modules with an ultraviolet intensity of 30000μW, installed at the bottom of the support 1. The number of these lamps corresponds to the number of experimental equipment placement positions on the support 1, totaling five, in a one-to-one relationship. The ultraviolet intensity sensor 15 monitors the ultraviolet intensity, automatically calculates the irradiation time based on the set ultraviolet irradiation dose, and promptly prompts for replacement when the ultraviolet lamp intensity decreases.

[0093] The drying system includes a PTC heating element 16, a drying fan 17, a semiconductor refrigeration module 18, a cooling fan 19, a water pump 20, a water storage tank 21, an exhaust fan 22, an exhaust port 23, a vacuum pump 24, a one-way valve 25, a waste liquid bottle 26, a waste liquid bottle level sensor 123, and a temperature and humidity sensor 27. There are two semiconductor refrigeration modules 18. One of the PTC heating element 16, the drying fan 17, the semiconductor refrigeration module 18, the exhaust fan 22, the exhaust port 23, and the temperature and humidity sensor 27 are placed in the sterilization chamber 301. The other semiconductor refrigeration module 18 includes a cooling fan 19 and a water pump 20. A water storage tank 21, an air pump 24, a one-way valve 25, a waste liquid bottle 26, and a waste liquid bottle level sensor 123 are placed in the component cavity 302. The semiconductor cooling module 18, the water pump 20, the water storage tank 21, the vent 23, the air pump 24, the one-way valve 25, and the waste liquid bottle 26 are connected by pipes 4. The semiconductor cooling module 18 is composed of a heat sink, a semiconductor cooling plate, and a water cooling plate that are sequentially bonded together and coated with thermally conductive adhesive. The cold water generated by the semiconductor cooling module 18 installed in the component cavity 302 circulates through the water cooling plate of the semiconductor cooling module 18 installed in the disinfection cavity 301 via pipes 4.

[0094] The control and display system includes a control circuit 28 and a touch screen 29. The control circuit 28 is connected to an infrared sensor 3, a two-position two-way solenoid valve 5, a peristaltic pump 6, a two-position three-way valve 7, a liquid level sensor 121 for disinfection and nucleic acid removal reagent bottles, a liquid level sensor 122 for cleaning reagent bottles, a liquid level sensor 123 for waste liquid bottles, an air pump 13, an ultraviolet lamp 14, an ultraviolet intensity sensor 15, a PTC heating element 16, a drying fan 17, a semiconductor refrigeration module 18, a cooling fan 19, a water pump 20, an exhaust fan 22, a vacuum pump 24, a temperature and humidity sensor 27, and a touch screen 29 via wiring.

[0095] Example 3

[0096] The method for cleaning, disinfecting, and removing nucleic acid from experimental equipment, using the apparatus described in Example 1, specifically includes the following steps:

[0097] (1) Connect the disinfection and nucleic acid removal reagent bottle 8 and the cleaning reagent bottle 9 to the pipe 4;

[0098] (2) Place one pipette on the support 1;

[0099] (3) Power-on self-test: including the functional status of each electronic component;

[0100] (4) Setting parameters: Set the cleaning, disinfection, nucleic acid removal, and drying parameters on the touchscreen 29, including reagent volume 1mL, spray time 10s, and ultraviolet irradiation dose 2200μW·min / cm². 2 Start operation when the endpoint temperature is 30℃ and the endpoint humidity is 50%.

[0101] (5) Chemical reagent disinfection and cleaning: Infrared sensor 3 monitors the position and quantity of experimental equipment, which is 1 pipette. Disinfection and nucleic acid removal reagent 10 and cleaning reagent 11 are sprayed sequentially through Venturi nozzle 2 in micron-level factors onto the surface and inner cavity of the pipette. The reagent volume of 1 mL and the spraying time of 10 s are automatically determined according to the set cleaning, disinfection and nucleic acid removal parameters.

[0102] (6) Ultraviolet disinfection: Turn on the ultraviolet lamp 14 and use ultraviolet disinfection in combination, according to the set ultraviolet irradiation dose of 2200 μW·min / cm 2 The monitored ultraviolet intensity of 30000μW automatically determines the irradiation time to be 30min;

[0103] (7) Automatic drying: The drying system is turned on. The drying fan 17 transfers the heat generated by the PTC heating element 16 to the sterilization chamber 301. The hot air is blown to the surface and inner cavity of the pipette through the Venturi nozzle 2. The semiconductor cooling module 18 condenses the mist into water droplets. The exhaust fan 22 blows the mist inside the sterilization chamber 301 out through the exhaust port 23. The air mist, residual reagents and condensed water droplets are discharged to the waste liquid bottle 26 through the air pump 24. The drying end time is automatically determined according to the set end temperature and humidity and by monitoring the temperature and humidity.

[0104] (8) End the operation.

[0105] Example 4

[0106] The method for cleaning, disinfecting, and removing nucleic acid from experimental equipment, using the apparatus described in Example 2, specifically includes the following steps:

[0107] (1) Connect the disinfection and nucleic acid removal reagent bottle 8 and the cleaning reagent bottle 9 to the pipe 4;

[0108] (2) Place 5 pipettes on stand 1;

[0109] (3) Power-on self-test: including the functional status of each electronic component;

[0110] (4) Setting parameters: Set the cleaning, disinfection, nucleic acid removal, and drying parameters on the touchscreen 29, including reagent volume 1mL, spray time 10s, and ultraviolet irradiation dose 2200μW·min / cm². 2 Start operation when the endpoint temperature is 30℃ and the endpoint humidity is 50%.

[0111] (5) Chemical reagent disinfection and cleaning: Infrared sensor 3 monitors the position and number of experimental instruments, which are 5 pipettes. Disinfection and nucleic acid removal reagent 10 and cleaning reagent 11 are sprayed sequentially through Venturi nozzle 2 in micron-level factors onto the surface and inner cavity of the experimental instruments. The reagent volume of 1 mL and the spraying time of 10 s are automatically determined according to the set cleaning, disinfection and nucleic acid removal parameters.

[0112] (6) Ultraviolet disinfection: Turn on the ultraviolet lamp 14 and use ultraviolet disinfection in combination, according to the set ultraviolet irradiation dose of 2200 μW·min / cm 2 The monitored ultraviolet intensity of 30000μW automatically determines the irradiation time to be 30min;

[0113] (7) Automatic drying: The drying system is turned on. The drying fan 17 transfers the heat generated by the PTC heating element 16 to the sterilization chamber 301. The hot air is blown to the surface and inner cavity of the pipette through the Venturi nozzle 2. The semiconductor cooling module 18 condenses the mist into water droplets. The exhaust fan 22 blows the mist inside the sterilization chamber 301 out through the exhaust port 23. The air mist, residual reagents and condensed water droplets are discharged to the waste liquid bottle 26 through the air pump 24. The drying end time is automatically determined according to the set end temperature and humidity and by monitoring the temperature and humidity.

[0114] (8) End the operation.

[0115] Performance testing

[0116] 1. Cleaning effect

[0117] (1) Experimental procedure: Place 5 pipettes on the support 1, clean and disinfect the pipettes to remove nucleic acid according to the method in Example 4, then rinse the pipettes with 10 mL of distilled water and collect the solution. Determine the hydrogen peroxide residue by xylenol orange method and the sodium dodecyl sulfate residue by spectrophotometry.

[0118] (2) Experimental results: as shown in Table 1.

[0119] Table 1. Cleaning test results (n=5)

[0120] Reagent Name hydrogen peroxide Sodium dodecyl sulfate Residual amount after cleaning / μg / L 0 0

[0121] As shown in Table 1, no hydrogen peroxide or sodium dodecyl sulfate residue remained on the pipettes after cleaning.

[0122] 2. Disinfection effect

[0123] (1) Experimental procedure: Place 5 pipettes on the support 1, and add 1 mL of the test bacterial suspension (10 mL) to each pipette. 5 -10 6 The solution (cfu / mL) was sprayed onto the surface and inner cavity of the pipette. The pipette was then cleaned, disinfected, and denucleated according to the method in Example 4. The pipette was then rinsed with 100 mL of sterile physiological saline, and the solution was collected and counted for microorganisms using the membrane filtration method.

[0124] (2) Experimental results: as shown in Table 2.

[0125] Table 2. Disinfection test results (n=5)

[0126] Test strains Escherichia coli Staphylococcus aureus Candida albicans Microbial count / CFU before disinfection <![CDATA[5.6×10 6 ]]> <![CDATA[2.1×10 6 ]]> <![CDATA[5.3×10 5 ]]> Microbial count after disinfection / CFU 0 0 0

[0127] As shown in Table 2, no microorganisms were detected on the pipettes after cleaning.

[0128] 3. Nucleic acid removal effect

[0129] (1) Experimental steps: Place 5 pipettes on the support 1, spray Escherichia coli genomic DNA solution onto the surface and inner cavity of the pipettes respectively, clean and disinfect the pipettes to remove nucleic acid according to the method in Example 4, then rinse the pipettes with 10 mL of distilled water and collect the solution, and detect DNA residue according to the PCR method.

[0130] (2) Experimental results: as shown in Table 3.

[0131] Table 3. Results of nucleic acid removal test (n=5)

[0132] PCR Escherichia coli genomic DNA Positive control + experimental group -

[0133] As shown in Table 3, no Escherichia coli genomic DNA remained on the cleaned pipette.

[0134] The above experiments demonstrate that this invention ensures the effectiveness of cleaning, disinfection, and nucleic acid removal without leaving any chemical reagent residues, thus achieving the cleaning, disinfection, and nucleic acid removal of experimental instruments such as pipettes.

[0135] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. In addition, those skilled in the art can combine and integrate the different embodiments or examples described in this specification.

[0136] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A device for washing, disinfecting and decontaminating nucleic acids from experimental instruments, characterized in that it comprises: The machine box (30) is a square cavity, comprising a component cavity (302), a sterilization cavity (301) and a sealing cover (303) connected in sequence from bottom to top, the top of the sterilization cavity (301) is sealed by the sealing cover (303), the sealing cover (303) is connected with the shell of the machine box (30) by a damping rotating shaft, is fixed at any angle of 0°-190°, and is inlaid with a sealing ring and a magnetic attraction in the shell of the machine box (30); The spray system comprises a support (1), a Venturi nozzle (2), an infrared sensor (3), a pipeline (4), a two-position two-way electromagnetic valve (5), a peristaltic pump (6), a two-position three-way valve (7), a sterilization nucleic acid removal reagent bottle (8), a cleaning reagent bottle (9), sterilization nucleic acid removal reagent (10), cleaning reagent (11), sterilization nucleic acid removal reagent bottle liquid level sensor (121), cleaning reagent bottle liquid level sensor (122) and air pump (13), the support (1), the Venturi nozzle (2) and the infrared sensor (3) are arranged in the sterilization cavity (301), the pipeline (4), the two-position two-way electromagnetic valve (5), the peristaltic pump (6), the two-position three-way valve (7), the sterilization nucleic acid removal reagent bottle (8), the cleaning reagent bottle (9), the sterilization nucleic acid removal reagent (10), the cleaning reagent (11), the sterilization nucleic acid removal reagent bottle liquid level sensor (121), the cleaning reagent bottle liquid level sensor (122) and the air pump (13) are arranged in the component cavity (302), the Venturi nozzle (2), the two-position two-way electromagnetic valve (5), the peristaltic pump (6), the two-position three-way valve (7), the sterilization nucleic acid removal reagent bottle (8), the cleaning reagent bottle (9) and the air pump (13) are connected by the pipeline (4) respectively, the sterilization nucleic acid removal reagent (10) is arranged in the sterilization nucleic acid removal reagent bottle (8), and the cleaning reagent (11) is arranged in the cleaning reagent bottle (9); The ultraviolet sterilization system comprises an ultraviolet lamp (14) and an ultraviolet intensity sensor (15), both arranged in the sterilization cavity (301); ​ The drying system comprises a PTC heating sheet (16), a drying fan (17), two semiconductor refrigeration modules (18), a heat dissipation fan (19), a water pump (20), a water storage tank (21), an exhaust fan (22), an exhaust hole (23), an air pump (24), a one-way valve (25), a waste liquid bottle (26), a waste liquid bottle liquid level sensor (123) and a temperature and humidity sensor (27), one of the PTC heating sheet (16), the drying fan (17), the semiconductor refrigeration module (18), the exhaust fan (22), the exhaust hole (23) and the temperature and humidity sensor (27) are arranged in the disinfection cavity (301), the other semiconductor refrigeration module (18), the heat dissipation fan (19), the water pump (20), the water storage tank (21), the air pump (24), the one-way valve (25), the waste liquid bottle (26) and the waste liquid bottle liquid level sensor (123) are arranged in the component cavity (302), and the semiconductor refrigeration module (18), the water pump (20), the water storage tank (21), the exhaust hole (23), the air pump (24), the one-way valve (25) and the waste liquid bottle (26) are connected through the pipeline (4) respectively. The control display system comprises a control circuit (28) and a touch screen (29), and the control circuit (28) is connected with the infrared sensor (3), the two-position two-way electromagnetic valve (5), the peristaltic pump (6), the two-position three-way valve (7), the disinfection nucleic acid reagent bottle liquid level sensor (121), the cleaning reagent bottle liquid level sensor (122), the waste liquid bottle liquid level sensor (123), the air pump (13), the ultraviolet lamp (14), the ultraviolet intensity sensor (15), the PTC heating sheet (16), the drying fan (17), the semiconductor refrigeration module (18), the heat dissipation fan (19), the water pump (20), the exhaust fan (22), the air pump (24), the temperature and humidity sensor (27) and the touch screen (29) through lines respectively.

2. The device for cleaning and disinfecting and removing nucleic acid from experimental apparatus according to claim 1, wherein The support (1) is provided with 1-10 experimental instrument placing positions with hook and groove structures and is inclined at an angle of 10°-90°.

3. The device for cleaning and disinfecting and removing nucleic acid from experimental apparatus according to claim 1, characterized in that, The Venturi nozzle (2) is composed of a conical drill bit with a circular cross section, a cylindrical throat, and a conical outlet, installed at the bottom of the support (1), the number of which is consistent with the number of experimental instrument placement positions on the support (1), in a one-to-one correspondence; the Venturi nozzle (2) is provided with anti-blocking spray holes, which are aligned with the internal cavities of the experimental instruments; the Venturi nozzle (2) is connected to the air pump (13) and the disinfection chamber (301) in sequence through the pipeline (4), forming a gas circuit with the disinfection chamber (301); the Venturi nozzle (2) is connected to the two-position two-way electromagnetic valve (5), the peristaltic pump (6), the two-position three-way valve (7), the disinfection nucleic acid removal reagent bottle (8), and the cleaning reagent bottle (9) in sequence through the pipeline (4), and the disinfection nucleic acid removal reagent (10) and the cleaning reagent (11) are micron-sized factor sprayed onto the surface and internal cavity of the experimental instrument through the spray holes.

4. The device for cleaning and disinfecting and removing nucleic acid from experimental apparatus according to claim 1, wherein The infrared sensor (3) is installed below the support (1), the number of which is consistent with the number of experimental instrument placement positions on the support (1), in a one-to-one correspondence.

5. The device for cleaning and disinfecting and removing nucleic acid from experimental instruments according to claim 1, characterized in that, The disinfection nucleic acid removal reagent bottle (8) and the cleaning reagent bottle (9) are blow-molded parts, with a volume of 100-2200 mL, including a bottle body, a sealing cap, one liquid outlet pipe, and one liquid inlet pipe, the bottle body is provided with a recessed handle, the sealing cap is composed of a threaded cap and a sealing disc, one liquid outlet pipe and one liquid inlet pipe are distributed on the sealing disc, and the sealing cap is tightly matched with the bottle body.

6. The device for cleaning and disinfecting and removing nucleic acid from laboratory equipment according to claim 1, characterized in that, The disinfection nucleic acid removal reagent (10) is preloaded in the disinfection nucleic acid removal reagent bottle (8), with a volume of 100-2200 mL, connected to two two-position three-way valves (7) and a peristaltic pump (6) in sequence through the pipeline (4), and then returned to the disinfection nucleic acid removal reagent bottle (8), forming a loop; in the loop, a three-way valve is connected to the two-position three-way valve (7) and the Venturi nozzle (2) in sequence through the pipeline (4), and the disinfection is sprayed by siphon effect.

7. The device for cleaning and disinfecting and removing nucleic acid from laboratory equipment according to claim 1, characterized in that, The cleaning reagent (11) is sterile purified water or water for injection; the cleaning reagent (11) is preloaded in the cleaning reagent bottle (9), with a volume of 100-2200 mL, connected to two two-position three-way valves (7) and a peristaltic pump (6) in sequence through the pipeline (4), and then returned to the cleaning reagent bottle (9), forming a loop; in the loop, a three-way valve is connected to the two-position three-way valve (7) and the Venturi nozzle (2) in sequence through the pipeline (4), and the cleaning is sprayed by siphon effect.

8. The device for cleaning and disinfecting and removing nucleic acid from experimental instruments according to claim 1, wherein, The ultraviolet lamp (14) is a UVC deep ultraviolet waterproof type sterilization and disinfection module, installed at the bottom of the support (1), the number of which is consistent with the number of experimental instrument placement positions on the support (1), in a one-to-one correspondence; the ultraviolet intensity sensor (15) is used to monitor the intensity of ultraviolet light, automatically calculate the irradiation time according to the set ultraviolet irradiation dose, and prompt to replace in time when the intensity of the ultraviolet lamp decreases.

9. The device for cleaning and disinfecting and removing nucleic acid from laboratory equipment according to claim 1, characterized in that, The semiconductor refrigeration module (18) is composed of radiating fins, semiconductor refrigeration sheets and water cooling sheets which are pasted together in sequence and between which there is heat-conducting glue; the cold water generated by the semiconductor refrigeration module (18) installed in the component cavity (302) circulates through the water cooling sheet of the semiconductor refrigeration module (18) installed in the sterilization cavity (301) through the pipeline (4).

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