Culture medium storage box for 5% carbon dioxide primary fibroblasts

Abstract

The utility model discloses a culture medium storage box for 5% carbon dioxide primary fibroblasts, and particularly relates to the technical field of carbon dioxide cell culture boxes, the culture medium storage box comprises a box body and a plurality of exhaust pipes placed at the bottom in the box body, and sponge sleeves sleeved on the outer walls of the exhaust pipes are attached to the bottom in the box body. A pipe hole and a sponge hole which are matched with each other are formed in the exhaust pipe and the sponge sleeve respectively, and part of the sponge sleeve covers the pipe hole. According to the culture medium storage box special for the fiber cells, the sponge sleeve is attached to the inner bottom of the box body, water drops are condensed in the box body and accumulated at the inner bottom of the box body, water is absorbed through the sponge sleeve, and then water on the sponge sleeve is taken away under the exhaust action of the exhaust pipe; therefore, evaporation of part of the condensed deionized water is accelerated, the deionized water is evaporated and used again, accumulation of the condensed water is reduced, and meanwhile the problem that the deionized water is discharged and wasted is solved.

Description

TECHNICAL FIELD

[0001] The utility model relates to carbon dioxide cell incubator technical field, concretely related to a kind of carbon dioxide primary fibroblast medium storage box of 52 percent. BACKGROUND

[0002] The storage box of carbon dioxide primary fibroblast special medium is a cell storage box, which needs to meet some specific requirements to ensure biological activity, so as to ensure the success and stability of cell culture. It includes temperature control, humidity control, CO2 concentration monitoring, light shielding design, and durability and cleanliness, so as to ensure the healthy culture of cells.

[0003] According to patent publication number CN220630850U, published on March 22, 2024, a refrigerated stem cell culture medium storage device is disclosed, which comprises: a refrigeration system inside an insulating shell, for maintaining the low temperature of the storage area; a temperature regulator integrated in the central control system, for accurately controlling the temperature of the storage area; the central control system also integrates a humidity regulator for adjusting the humidity level of the storage area; a layered structure is added inside the storage area, using movable shelves or plates to accommodate culture dishes or containers; a data recording system is integrated in the central control system.

[0004] In the prior art including the above-mentioned patent, the current common cell culture medium storage box can provide specific humidity, temperature and carbon dioxide concentration for the storage of primary fibroblast cells. However, for the storage of cultured cells in the medium, the storage temperature is generally 2-8°C. Although the existing medium storage box can provide suitable temperature, temperature and carbon dioxide concentration for the storage of the medium, compared with room temperature, 2-8°C is a relatively low temperature. In order to maintain the biological activity of cells, the inside of the storage box has a certain humidity, and the inner wall of the storage box is made of stainless steel plate. Water droplets are easily condensed on the stainless steel plate and flow down the inner wall of the stainless steel plate to the bottom of the box. The accumulated water is not easy to drain. If the deionized water used in the storage box is directly drained, the drained water cannot be directly used due to contamination, which leads to waste of deionized water. UTILITY MODEL CONTENTS

[0005] The utility model aims to provide a carbon dioxide primary fibroblast medium storage box of 52 percent, which can effectively utilize condensed deionized water and reduce the accumulation of water in the storage box.

[0006] In order to achieve the above object, the utility model provides the following technical scheme: a culture medium storage box for 52 percent carbon dioxide primary fibroblast, including box body and a plurality of exhaust pipes placed in the bottom of the box body, the sponge cover of the exhaust pipe outer wall is attached to the bottom of the box body, the exhaust pipe and the sponge cover are respectively provided with pipe holes and sponge holes matched with each other, and part of the sponge cover covers the pipe hole.

[0007] As preferred, the mounting plate is further provided, and a plurality of threaded grooves are formed on the mounting plate and are respectively threadedly assembled with the first ends of the exhaust pipes.

[0008] As preferred, the dismounting piece and a jack are further provided, and the second ends of the exhaust pipes are inserted into the jack.

[0009] As preferred, the inner wall of the jack is provided with a stop ring, and the inner wall of the jack is provided with a rubber ring.

[0010] As preferred, the mounting piece is fixedly installed in the box body, the protruding part on the mounting piece is slidably arranged in the sliding groove formed on the dismounting piece, and the dismounting piece and the mounting piece are surrounded to form an air cavity.

[0011] As preferred, the outer wall of the mounting piece is provided with an air inlet, the air side box is fixedly installed on the box body, the air side box and the air inlet are in communication with each other, and the centrifugal fan is fixedly installed in the air side box.

[0012] As preferred, the upper air inlet is formed on the mounting piece.

[0013] As preferred, the pipe holes and the sponge holes are respectively directed upward.

[0014] As preferred, the limiting plate is fixedly installed on the dismounting piece, the supporting plate is fixedly installed on the mounting plate, and the water box is movably arranged on the dismounting piece and the supporting plate.

[0015] As preferred, the exhaust pipe is a stainless steel pipe, and the sponge cover is a water-absorbing sponge.

[0016] In the above technical scheme, the culture medium storage box for 52 percent carbon dioxide primary fibroblast has the following beneficial effects: by attaching the sponge cover to the bottom of the box body, when the water droplets are condensed in the box body and accumulated in the bottom of the box body, the water body is absorbed by the sponge cover, and then the water on the sponge cover is taken away under the action of the exhaust pipe, so that the evaporation of the condensed part of the deionized water is accelerated, the deionized water is used again, the accumulation of the condensed water is reduced, and the problem of waste of deionized water is avoided. BRIEF DESCRIPTION OF DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present application or the prior art, the drawings needed to be used in the embodiments will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments described in the present application, and other drawings can also be obtained by those skilled in the art based on these drawings.

[0018] Figure 1 The box overall structure schematic view provided by the embodiment of the present application is shown in the figure.

[0019] Figure 2 The mounting plate and exhaust pipe explosion structure schematic view provided by the embodiment of the present application is shown in the figure.

[0020] Figure 3 The structure schematic view when the dismounting piece and the mounting piece are spliced provided by the embodiment of the present application is shown in the figure.

[0021] Figure 4 The box cross-section structure schematic view provided by the embodiment of the present application is shown in the figure.

[0022] Figure 5 The A place enlarged structure schematic view provided by the embodiment of the present application is shown in the figure.

[0023] Explanation of reference signs:

[0024] 1, box; 2, water box; 3, dismounting piece; 4, mounting piece; 5, exhaust pipe; 6, sponge cover; 7, mounting plate; 11, wind side box; 12, centrifugal fan; 31, limiting plate; 32, sliding groove; 33, insertion hole; 34, blocking ring; 35, rubber ring; 36, wind cavity; 41, upper air inlet; 42, protruding part; 43, air inlet; 51, pipe hole; 61, sponge hole; 71, threaded groove; 72, supporting plate. DETAILED DESCRIPTION

[0025] In order to make the skilled in the art better understand the technical solutions of the present application, the present application will be further described in detail in combination with the drawings.

[0026] As Figures 1-5As shown in the figure, a kind of carbon dioxide primary fibroblast medium storage box of 52%, including box 1 and the multiple exhaust pipes 5 placed in the bottom of box 1, the outer wall of exhaust pipe 5 is sleeved with sponge cover 6, which is attached to the bottom of box 1, and the pipe hole 51 and the sponge hole 61 are respectively formed on the exhaust pipe 5 and the sponge cover 6, and part of the sponge cover 6 covers on the pipe hole 51. By making the sponge cover 6 attached to the bottom of the box 1, and then condensing water droplets in the box 1 and accumulating in the bottom of the box 1, the water in the sponge cover 6 is absorbed by the sponge cover 6, and then the water in the sponge cover 6 is taken away by the exhaust pipe 5, so that the part of the deionized water is evaporated, so that the deionized water is evaporated again, and the accumulation of condensed water is reduced, and the problem of waste of deionized water is avoided.

[0027] Specifically, the diameter of the pipe hole 51 is greater than the diameter of the sponge hole 61, and the hole axes of the pipe hole 51 and the sponge hole 61 are consistent, when the pipe hole 51 exhausts, the part of the sponge cover 6 on the pipe hole 51 air port is blown by the fast flowing wind, so that the evaporation of the water on the sponge cover 6 is accelerated.

[0028] As further provided by the utility model, further comprising mounting plate 7, and the multiple threaded grooves 71 formed on mounting plate 7 are respectively and first end of exhaust pipe 5 threaded assembly.

[0029] Specifically, as shown in the figure, the exhaust pipe 5 is threaded assembled on the mounting plate 7, so that the exhaust pipe 5 can be easily disassembled, cleaned and disinfected, and the multiple exhaust pipes 5 can be simultaneously installed by taking the mounting plate 7. Figure 2

[0030] Further, it comprises disassembling piece 3 and jack 33 formed on one side of the outer wall of disassembling piece 3, and the second end of exhaust pipe 5 is inserted into jack 33.

[0031] Specifically, by taking the mounting plate 7 and inserting the second end of the multiple exhaust pipes 5 into the jack 33, the multiple exhaust pipes 5 can be simultaneously blown by blowing the disassembling piece 3, and the jack 33 limits and guides the exhaust pipe 5.

[0032] Further, the inner wall of the jack 33 is provided with a stop ring 34, and the inner wall of the jack 33 is provided with a rubber ring 35.

[0033] Specifically, when the second end of the exhaust pipe 5 is inserted into the jack 33, the stop ring 34 limits the exhaust pipe 5, and the extrusion between the rubber ring 35 and the exhaust pipe 5 enables the exhaust pipe 5 to be limited on the disassembling piece 3, so as to avoid displacement of the exhaust pipe 5 when blowing.

[0034] ​Further, the mounting member 4 is fixedly installed in the box 1, and a protruding portion 42 on the mounting member 4 is slidingly arranged in a sliding groove 32 formed on the dismounting member 3, and the dismounting member 3 and the mounting member 4 are enclosed to form the air cavity 36.

[0035] Specifically, the air cavity 36 formed between the dismounting member 3 and the mounting member 4 is used to facilitate air blowing along the air cavity 36 to the multiple exhaust pipes 5 when air is blown into the air cavity 36, and the dismounting member 3 is slidingly arranged between the dismounting member 3 and the mounting member 4 to facilitate subsequent quick dismounting of the dismounting member 3 on the mounting member 4 for cleaning and disinfection, and the mounting member 4 is exposed to facilitate disinfection of the mounting member 4.

[0036] Further, an air inlet 43 is formed on an outer wall of one side of the mounting member 4, an air side box 11 is fixedly installed on the box 1, the air side box 11 and the air inlet 43 are in communication with each other, and a centrifugal fan 12 is fixedly installed in the air side box 11.

[0037] Specifically, the centrifugal fan 12 is used to blow air into the air inlet 43 to facilitate air entering the air cavity 36, as shown in Figure 4 The air inlet and the air outlet of the air side box 11 are in communication with the box 1, thereby realizing air circulation and avoiding carbon dioxide leakage.

[0038] Further, an upper air inlet 41 is formed on the mounting member 4.

[0039] Specifically, as shown in Figure 5 The upper air inlet 41 on the mounting member 4 is used to accelerate air flow in the box 1 to make carbon dioxide more evenly distributed.

[0040] Further, the pipe hole 51 and the sponge hole 61 are respectively oriented upward.

[0041] Specifically, since the exhaust pipe 5 and the sponge sleeve 6 are located at the bottom of the box 1, and carbon dioxide has a density greater than that of air to sink, the pipe hole 51 is oriented upward to blow air to drive carbon dioxide at the bottom of the box 1 to rise, thereby being more evenly distributed in the box 1.

[0042] Further, a limiting plate 31 is fixedly installed on the dismounting member 3, a supporting plate 72 is fixedly installed on the mounting plate 7, and the water box 2 is movably arranged on the dismounting member 3 and the supporting plate 72.

[0043] Specifically, the water box 2 is placed on the dismounting member 3 and the supporting plate 72 to support the water box 2, and evaporation of water in the water box 2 can increase the humidity in the box 1.

[0044] Further, the exhaust pipe 5 is a stainless steel pipe, and the sponge sleeve 6 is a water-absorbing sponge.

[0045] Working principle: through the sponge cover 6 is adhered to the box 1 inner bottom, and then in the box 1 inside condensation water droplets and collection in the box 1 inner bottom, through the sponge cover 6 to absorb water, and then under the action of exhaust pipe 5 exhaust takes away the moisture on the sponge cover 6, thereby accelerating the condensation of part of the deionized water evaporation, so that the deionized water is evaporated again, reduce the condensation water accumulation, at the same time avoid the deionized water discharge waste problem.

[0046] The above only describes certain exemplary embodiments of the present application by way of illustration, without doubt, for those skilled in the art, without departing from the spirit and scope of the present application, the described embodiments can be modified in various ways. Therefore, the above drawings and description are illustrative in nature and should not be construed as limiting the scope of protection of the present application.

Claims

1. A 52% carbon dioxide primary fibroblast cell culture medium storage tank, characterized by, The utility model provides a kind of air exhaust pipe and water box, including box (1) and the multiple air exhaust pipes (5) of placing in the bottom of box (1), the sponge cover (6) of outer wall of the air exhaust pipe (5) is attached to the bottom of box (1), the pipe hole (51) and sponge hole (61) respectively matched with are set on the air exhaust pipe (5) and sponge cover (6), and part of sponge cover (6) covers on pipe hole (51).

2. The 52% carbon dioxide primary fibroblast cell culture medium storage box according to claim 1, characterized in that, Further comprising mounting plate (7), and multiple threaded grooves (71) set on mounting plate (7) are respectively and the first end of air exhaust pipe (5) threaded assembly.

3. The 52% carbon dioxide primary fibroblast cell culture medium storage box according to claim 2, characterized in that, Including dismounting piece (3) and jack (33) set on the outer wall of one side of dismounting piece (3), the second end of air exhaust pipe (5) is inserted into jack (33).

4. The 52% carbon dioxide primary fibroblast cell culture medium storage box according to claim 3, characterized in that, The inner wall of jack (33) is provided with stop ring (34), and the inner wall of jack (33) is provided with rubber ring (35).

5. The 52% carbon dioxide primary fibroblast cell culture medium storage box according to claim 3, characterized in that, Including mounting piece (4) fixedly installed in box (1), and the convex part (42) on mounting piece (4) is slidably arranged in the sliding slot (32) set on dismounting piece (3), and wind cavity (36) is formed between dismounting piece (3) and mounting piece (4).

6. The 52% carbon dioxide primary fibroblast cell culture medium storage box according to claim 5, characterized in that, The outer wall of one side of mounting piece (4) is provided with air inlet (43), and air side box (11) is fixedly installed on box (1), air side box (11) and air inlet (43) are communicated with each other, and centrifugal fan (12) is fixedly installed in air side box (11).

7. The 52% carbon dioxide primary fibroblast cell culture medium storage box according to claim 6, characterized in that, The upper air inlet (41) is set on mounting piece (4).

8. The 52% carbon dioxide primary fibroblast cell culture medium storage box according to claim 1, characterized in that, The pipe hole (51) and sponge hole (61) are respectively towards upward.

9. The 52% carbon dioxide primary fibroblast cell culture medium storage box according to claim 3, characterized in that, Limiting plate (31) is fixedly installed on dismounting piece (3), and supporting plate (72) is fixedly installed on mounting plate (7), and water box (2) is movably arranged on dismounting piece (3) and supporting plate (72).

10. The 52% carbon dioxide primary fibroblast cell culture medium storage box according to claim 1, characterized in that, The air exhaust pipe (5) is stainless steel pipe, and the sponge cover (6) is water-absorbing sponge.

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