Intestinal anaerobic microorganism separation culture device
By combining a sealed glove box and a purification deoxygenation tank with inert gas replacement and deoxygenating agent, the problems of complex operation and residual oxygen in existing anaerobic microbial isolation and culture equipment are solved, achieving efficient creation of an anaerobic environment and promoting microbial growth.
Patent Information
- Application Number
- CN202422799065.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-18
- Publication Date
- 2026-01-02
- Estimated Expiration
- 2034-11-18
AI Technical Summary
Existing technologies make it difficult to achieve complete isolation and culture of anaerobic microorganisms. Traditional methods are cumbersome to operate, require sophisticated equipment, are expensive, and are difficult to completely remove residual oxygen from the container.
A sealed glove box and a purification deoxygenation tank are used in combination with inert gas replacement and deoxygenating agent. After most of the oxygen is replaced by inert gas, the deoxygenating agent is used to further remove the residual oxygen. Combined with air pressure and oxygen content monitors to control airflow, an anaerobic environment is ensured.
It effectively reduces the oxygen content inside the sealed glove box, creating a more suitable environment for the growth of anaerobic microorganisms and improving the success rate of isolation and culture.
Smart Images
Figure CN223752728U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model belongs to the technical field of microorganism culture, especially relates to an intestinal anaerobic microorganism separation culture device. BACKGROUND
[0002] In the field of modern biotechnology, the research and application of anaerobic microorganisms have been increasingly valued. These microorganisms widely exist in nature, such as soil, water, and animal intestines, and they play an important role in material circulation, energy conversion, and environmental protection.
[0003] However, due to the sensitivity of anaerobic microorganisms to oxygen, the separation and culture process is relatively complex, and special equipment and technical means are required. Traditional methods for separating and culturing anaerobic microorganisms mainly include physical, chemical and biological methods. Although most of the methods can achieve the separation and culture of anaerobic microorganisms to some extent, they generally have problems such as complicated operation, high equipment requirements, and high cost. Among them, the physical method includes a method of replacing gas to create an anaerobic environment, i.e. replacing the oxygen in the container with inert gases such as carbon dioxide, hydrogen, nitrogen, and argon. The operation is simple and the cost is low. However, it is difficult to completely replace the oxygen due to the complexity of gas diffusion and the presence of small pores, corners, etc. in the container, and some residual oxygen is difficult to completely remove, making it difficult to achieve an anaerobic culture environment for anaerobic microorganisms.
[0004] Therefore, an intestinal anaerobic microorganism separation culture device is proposed to overcome the shortcomings of the prior art. INVENTION CONTENTS
[0005] To solve the above technical problems, the utility model provides an intestinal anaerobic microorganism separation culture device.
[0006] To achieve the above purpose, the utility model provides an intestinal anaerobic microorganism separation culture device, which comprises a sealed glove box, a first air inlet structure is formed on one side of the sealed glove box, and a first air outlet structure is formed on the end of the sealed glove box away from the first air inlet structure; a purification oxygen removal tank is arranged in the sealed glove box, and an oxygen removal agent is arranged in the purification oxygen removal tank.
[0007] According to the intestinal anaerobic microorganism separation culture device provided by the utility model, a first oxygen content monitor and a first air pressure monitor are also fixedly arranged in the sealed glove box, the first oxygen content monitor and the first air pressure monitor are electrically connected with a controller, and the first air inlet structure and the first air outlet structure are controlled by the controller.
[0008] According to the utility model provide a kind of intestinal anaerobic microorganism separation culture device, the transition bin is fixed on one side of the sealed glove box, the transition bin one end is embedded in the inside of the sealed glove box, and is communicated with the sealed glove box by inner sealing door, the transition bin other end is located the outside of the sealed glove box, and is communicated with outside by outer sealing door;Second air inlet structure is set in the top of the transition bin, and second air outlet structure is set in the bottom.
[0009] According to the utility model provide a kind of intestinal anaerobic microorganism separation culture device, second oxygen content monitor and second air pressure monitor are also fixedly arranged in the sealed glove box, and the second air inlet structure and the second air outlet structure are controlled by the controller.
[0010] According to the utility model provide a kind of intestinal anaerobic microorganism separation culture device, illumination lamp and ultraviolet lamp are fixedly connected on the top in the sealed glove box.
[0011] According to the utility model provide a kind of intestinal anaerobic microorganism separation culture device, the first air outlet structure includes two air outlet holes, first manual air outlet valve and first automatic air outlet valve are fixedly connected in two air outlet holes respectively, and the first automatic air outlet valve is electrically connected with the controller.
[0012] The second air inlet structure includes two air inlet holes, second manual air inlet valve and second automatic air inlet valve are fixedly connected in two air inlet holes respectively, and the second automatic air inlet valve is electrically connected with the controller.
[0013] According to the utility model provide a kind of intestinal anaerobic microorganism separation culture device, large bin door is set in the side of the sealed glove box.
[0014] According to the utility model provide a kind of intestinal anaerobic microorganism separation culture device, storage cabinet is fixedly connected in the bottom of the sealed glove box, and a plurality of universal wheels are fixedly connected in the bottom of the storage cabinet.
[0015] According to the utility model provide a kind of intestinal anaerobic microorganism separation culture device, circulation fan is fixedly connected in the sealed glove box.
[0016] Compared with prior art, the utility model has following advantages and technical effects:
[0017] When the oxygen in the sealed glove box is replaced, the first air inlet structure is communicated with the gas source of external inert gas, the inert gas can be nitrogen, hydrogen or mixed gas, etc., the first air outlet structure is opened, the inert gas enters from the first air inlet structure, drives the gas in the sealed glove box to move to the first air outlet structure, and then is discharged from the first air outlet structure, so that the displacement replacement of most of the oxygen in the sealed glove box is realized, and then the first air outlet structure is closed; for the small amount of residual oxygen, the operator inserts hands into the gloves of the sealed glove box, opens the oxygen removal tank, and the oxygen scavenger in the oxygen removal tank contacts the oxygen in the sealed glove box to remove the oxygen; the inert gas replacement and the subsequent oxygen scavenger oxygen removal of the utility model further reduce the content of oxygen in the sealed glove box, create a better anaerobic microbial separation and culture environment, and are more conducive to the growth and reproduction of anaerobic microorganisms. BRIEF DESCRIPTION OF DRAWINGS
[0018] The drawings constituting a part of the present application are used to provide further understanding of the present application, the illustrative embodiments of the present application and the description thereof are used to explain the present application, and do not constitute improper limitation on the present application. In the drawings:
[0019] Fig. 1 It is a structure schematic view of the intestinal anaerobic microbial separation and culture device of the utility model;
[0020] Fig. 2 It is an internal structure schematic view of the sealed glove box in the utility model;
[0021] Fig. 3 It is an internal structure schematic view of the transition bin in the utility model.
[0022] In the drawings: 1, sealed glove box; 2, first air inlet structure; 3, first air outlet structure; 4, oxygen removal tank; 5, first oxygen content monitor; 6, first air pressure monitor; 7, transition bin; 8, outer sealing door; 9, inner sealing door; 10, second air inlet structure; 11, second air outlet structure; 12, illuminating lamp; 13, ultraviolet lamp; 14, large bin door; 15, storage cabinet; 16, universal wheel; 18, second oxygen content monitor; 19, second air pressure monitor. DETAILED DESCRIPTION
[0023] The technical scheme in the embodiments of the utility model will be clearly and completely described below in combination with the drawings in the embodiments of the utility model, and obviously, the described embodiments only are part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor belong to the protection scope of the utility model.
[0024] In order to make the above-mentioned purposes, features and advantages of the present application more apparent and understandable, the present application will be further described in detail below with reference to the drawings and specific embodiments.
[0025] Referring to Figs. 1 to 3 As shown in the figure, the present embodiment provides an intestinal anaerobic microorganism separation and culture device, which comprises: a sealed glove box 1, the sealed glove box 1 is provided with a first air inlet structure 2 on one side, and a first air outlet structure 3 is correspondingly provided at one end of the sealed glove box 1 away from the first air inlet structure 2; a purification and oxygen removal tank 4 is arranged in the sealed glove box 1, and an oxygen removal agent is arranged in the purification and oxygen removal tank 4.
[0026] When the oxygen in the sealed glove box 1 is replaced, the first air inlet structure 2 is connected to the gas source of external inert gas, which can be nitrogen, hydrogen or a mixture thereof, etc., the first air outlet structure 3 is opened, the inert gas enters from the first air inlet structure 2, drives the gas in the sealed glove box 1 to move towards the first air outlet structure 3, and then is discharged from the first air outlet structure 3, so as to realize the displacement replacement of most of the oxygen in the sealed glove box 1, and then the first air outlet structure 3 is closed; for the remaining small amount of oxygen, the operator inserts his hands into the gloves of the sealed glove box 1, opens the purification and oxygen removal tank 4, and the oxygen removal agent in the purification and oxygen removal tank 4 contacts the oxygen in the sealed glove box 1 to remove the oxygen; the present application further reduces the content of oxygen in the sealed glove box 1 through inert gas replacement and subsequent oxygen removal of the oxygen removal agent, and creates a better anaerobic microorganism separation and culture environment, which is more conducive to the growth and reproduction of anaerobic microorganisms.
[0027] The oxygen removal agent can be reducing iron powder or a mixture of reducing iron powder and sodium chloride, etc., at normal temperature, iron Fe reacts with oxygen O2 in the air to generate iron oxide, thereby removing oxygen, and sodium chloride can accelerate the reaction and improve the oxygen removal speed.
[0028] According to the intestinal anaerobic microorganism separation and culture device provided by the present embodiment, a first oxygen content monitor 5 and a first air pressure monitor 6 are further fixedly arranged in the sealed glove box 1, the first oxygen content monitor 5 and the first air pressure monitor 6 are electrically connected with a controller, and the first air inlet structure 2 and the first air outlet structure 3 are controlled by the controller.
[0029] The first oxygen content monitor 5 can monitor the oxygen content in the sealed glove box 1, so that the staff can know the oxygen content in the sealed glove box 1 in real time, the first air pressure monitor 6 can react to the air pressure in the sealed glove box 1 in real time, and transmit the air pressure information to the controller, the controller adjusts the air flow at the first air inlet structure 2 and the first air outlet structure 3 according to the real-time air pressure, so as to keep the air pressure stable and easily obtain a better oxygen replacement effect.
[0030] According to the intestinal anaerobic microorganism separation culture device provided by the embodiment, the transition bin 7 is fixed on one side of the sealed glove box 1, one end of the transition bin 7 is embedded in the inside of the sealed glove box 1, and the transition bin 7 is in communication with the inside of the sealed glove box 1 through the inner sealing door 9, and the other end of the transition bin 7 is located outside the sealed glove box 1 and is in communication with the outside through the outer sealing door 8; the second air inlet structure 10 is arranged at the top end of the transition bin 7, and the second air outlet structure 11 is arranged at the bottom end of the transition bin 7.
[0031] In order to avoid mixing of external oxygen when the anaerobic microorganism is added after the oxygen replacement of the sealed glove box 1 is completed, the transition bin 7 is arranged, the anaerobic microorganism first enters the transition bin 7 through the outer sealing door 8, and then the transition bin 7 is closed, the oxygen in the transition bin 7 is replaced through the second air inlet structure 10 and the second air outlet structure 11, then the operator opens the inner sealing door 9 from the inside of the sealed glove box 1, and the anaerobic microorganism is taken into the sealed glove box 1, so as to minimize the entry of external oxygen and maintain the oxygen-free or micro-oxygen environment in the sealed glove box 1. A small amount of oxygen entering can also be removed by the deoxidizing agent in the deoxidizing tank 4 to ensure the survival environment of the anaerobic microorganism.
[0032] According to the intestinal anaerobic microorganism separation culture device provided by the embodiment, the second air pressure monitor 18 and the second air pressure monitor 19 are further fixedly arranged in the sealed glove box 1, and the second air inlet structure 10 and the second air outlet structure 11 are controlled by the controller.
[0033] Similarly, the second air pressure monitor 18 and the second air pressure monitor 19 can detect the oxygen content and air pressure in the transition bin 7 in real time and transmit the same to the controller, and the controller controls the air flow of the second air inlet structure 10 and the second air outlet structure 11 to maintain stable air pressure and better oxygen replacement effect.
[0034] According to the intestinal anaerobic microorganism separation culture device provided by the embodiment, the illumination lamp 12 and the ultraviolet lamp 13 are fixedly arranged at the top of the sealed glove box 1.
[0035] The illumination lamp 12 is used for illumination in the sealed glove box 1, and the ultraviolet lamp 13 is used for sterilization.
[0036] According to the intestinal anaerobic microorganism separation culture device provided by the embodiment, the first air outlet structure 3 includes two air outlet holes, and the first manual air outlet valve and the first automatic air outlet valve are fixedly arranged in the two air outlet holes, respectively, and the first automatic air outlet valve is electrically connected with the controller.
[0037] The second air inlet structure 10 includes two air inlet holes, and the second manual air inlet valve and the second automatic air inlet valve are fixedly arranged in the two air inlet holes, respectively, and the second automatic air inlet valve is electrically connected with the controller.
[0038] The first air outlet structure 3 and the first air inlet structure 2 are both provided with two air inlet and outlet modes of manual and automatic, the manual mode is more convenient and efficient, the automatic mode is convenient for being controlled by the controller, control is more accurate, and the replacement effect is better.
[0039] Correspondingly, the second air outlet structure 11 and the second air inlet structure 10 are also respectively provided with two air inlet and outlet modes of manual and automatic, and are controlled by the controller.
[0040] According to the intestinal anaerobic microorganism separation and culture device provided in the embodiment, the large door 14 is arranged on the side of the sealed glove box 1.
[0041] The large door 14 is used for the access when larger articles are placed into the sealed glove box 1, and is normally in a sealed closed state, for example, when an article placing rack, a centrifuge and the like are placed into the sealed glove box 1, the large door 14 is opened.
[0042] According to the intestinal anaerobic microorganism separation and culture device provided in the embodiment, the storage cabinet 15 is fixedly connected to the bottom of the sealed glove box 1, and the plurality of universal wheels 16 are fixedly connected to the bottom of the storage cabinet 15.
[0043] The storage cabinet 15 is fixedly connected to the bottom of the sealed glove box 1, so that the articles are conveniently taken and the circuit is conveniently arranged; and the universal wheels 16 are convenient for moving.
[0044] According to the intestinal anaerobic microorganism separation and culture device provided in the embodiment, the circulating fan is fixedly connected in the sealed glove box 1.
[0045] The circulating fan can make the gas in the sealed glove box 1 flow, and is used when the oxygen is removed by opening the purification oxygen removal tank 4, so that the oxygen is quickly contacted with the oxygen removal agent, and the oxygen removal efficiency is improved.
[0046] The not-yet-exhausted parts of the utility model are all conventional technical means known by the skilled in the art.
[0047] In the description of the utility model, it is understood that the orientation or position relationship indicated by the terms "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like is the orientation or position relationship shown in the drawings, and is only for the convenience of describing the utility model, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, therefore, it cannot be understood as a limitation on the utility model.
[0048] The above-described embodiments only describe the preferred modes of the utility model, and do not limit the scope of the utility model, and various deformations and improvements of the technical solutions of the utility model made by the ordinary skilled in the art without departing from the design spirit of the utility model should fall within the protection scope determined by the claims of the utility model.
Claims
1. A device for isolating and culturing intestinal anaerobic microorganisms, characterized in that, The utility model relates to a sealed glove box, which comprises: A sealed glove box (1) is provided with a first air inlet structure (2) on one side, and a first air outlet structure (3) is correspondingly provided at the end of the sealed glove box (1) away from the first air inlet structure (2); a deoxidizing tank (4) is arranged in the sealed glove box (1), and a deoxidizing agent is arranged in the deoxidizing tank (4).
2. The device according to claim 1, wherein: A first oxygen content monitor (5) and a first air pressure monitor (6) are fixedly arranged in the sealed glove box (1), and the first oxygen content monitor (5) and the first air pressure monitor (6) are electrically connected with a controller; the first air inlet structure (2) and the first air outlet structure (3) are controlled by the controller.
3. The device according to claim 2, wherein: A transition bin (7) is fixedly connected to one side of the sealed glove box (1), one end of the transition bin (7) is embedded in the sealed glove box (1), and the transition bin (7) is in communication with the sealed glove box (1) through an inner sealing door (9); the other end of the transition bin (7) is located outside the sealed glove box (1) and is in communication with the outside through an outer sealing door (8); a second air inlet structure (10) is formed at the top end of the transition bin (7), and a second air outlet structure (11) is formed at the bottom end of the transition bin (7).
4. The device according to claim 3, wherein: A second oxygen content monitor (18) and a second air pressure monitor (19) are fixedly arranged in the sealed glove box (1), and the second air inlet structure (10) and the second air outlet structure (11) are controlled by the controller.
5. The device according to claim 1, wherein: A lighting lamp (12) and an ultraviolet lamp (13) are fixedly connected to the top of the sealed glove box (1).
6. The device according to claim 3, wherein: The first air outlet structure (3) comprises two air outlet holes, a first manual air outlet valve and a first automatic air outlet valve are fixedly connected to the two air outlet holes respectively, and the first automatic air outlet valve is electrically connected with the controller; The second air inlet structure (10) comprises two air inlet holes, a second manual air inlet valve and a second automatic air inlet valve are fixedly connected to the two air inlet holes respectively, and the second automatic air inlet valve is electrically connected with the controller.
7. The device according to claim 1, wherein: A large bin door (14) is formed on the side of the sealed glove box (1).
8. The device according to claim 1, wherein: A storage cabinet (15) is fixedly connected to the bottom of the sealed glove box (1), and a plurality of universal wheels (16) are fixedly connected to the bottom of the storage cabinet (15).
9. The device according to claim 1, wherein: A circulating fan is fixedly connected in the sealed glove box (1).