Microfluidic sperm optimization device

Abstract

The utility model relates to an optimization device in the aspect of micro-fluidic sperm optimization, belongs to the technical field of medical instruments, and solves the problem of low concentration of high-quality sperms after sperm optimization in the prior art. The fallopian tube simulating device comprises a collecting pool and a screening piece, the screening piece is connected with the collecting pool, a screening channel is arranged on the screening piece and provided with an inlet end and an outlet end, the inlet end can be soaked in original semen, the outlet end is connected with the collecting pool, and the fallopian tube is simulated through the screening channel. And performing sperm screening by using the boundary tracking navigation capability of the sperms. According to the sperm screening device, the screening piece is infiltrated into the original sperm, the fallopian tube is simulated through the screening channel, sperm screening is carried out through boundary tracking navigation capacity of sperms, operation is simple, centrifugal operation is not needed in the screening process, sperm DNA cannot be damaged or broken due to peroxidation, the concentration of screened high-quality sperms is improved, and then the fertilization rate is increased.

Description

TECHNICAL FIELD

[0001] The utility model relates to medical instrument technical field especially relates to micro -fluidic sperm optimal selection device. BACKGROUND

[0002] Infertility affects the global childbearing age population, and the proportion increases year by year. The in vitro fertilization technology of assisted reproductive technology is to help patients to help pregnancy treatment by using optimized sperm and oocyte combination. The commonly used sperm optimization technology in the present stage has upstream method and density gradient centrifugation method, and the two methods are time-consuming and need centrifugal treatment, and centrifugal treatment can easily cause sperm DNA peroxidation damage and fracture, thereby leading to low concentration of high-quality sperm, and further leading to fertilization failure. CONTENT OF THE UTILITY MODEL

[0003] In view of the above analysis, the utility model aims at providing micro -fluidic sperm optimal selection device to solve the problem of low concentration of high-quality sperm after sperm optimization in prior art.

[0004] The utility model discloses a purpose mainly through following technical scheme realizes:

[0005] Micro -fluidic sperm optimal selection device, its characterized in being including collection pool and screening piece, the screening piece is connected with collection pool, be equipped with screening channel on the screening piece, the screening channel has inlet end and outlet end, the inlet end can be soaked into raw semen, and the outlet end is connected with collection pool, and the screening channel simulates oviduct, and sperm screening is carried out using the boundary tracking navigation ability of sperm;The diameter of the outlet end of the screening channel gradually becomes smaller, can prevent high-quality sperm screened into the collection pool from swimming in the screening channel.

[0006] Further, the screening piece is provided with a stepped contact surface, which can increase the contact surface between the screening piece and the raw semen.

[0007] Further, the stepped contact surface includes a first stepped contact surface and a second stepped contact surface.

[0008] Further, the first stepped contact surface is located away from the axis of the screening piece.

[0009] Further, the second stepped contact surface 2 is located close to the axis of the screening piece.

[0010] Further, the screening channel is a capillary tube.

[0011] Further, the screening channel is provided with a plurality of.

[0012] Further, it further includes a handle.

[0013] Further, the handle is installed in the collection pool.

[0014] Further, the handle is a telescopic handle.

[0015] The technical scheme of the utility model can realize at least one of the following effects:

[0016] (1) The utility model discloses a microfluidic sperm optimization device, which comprises a collecting pool and a screening element, the screening element is connected with the collecting pool, a screening channel is arranged on the screening element, the screening channel has an inlet end and an outlet end, the inlet end can be immersed in raw semen, the outlet end is connected with the collecting pool, the screening channel simulates a fallopian tube, and sperm screening is performed by using the boundary tracking navigation ability of sperm.

[0017] (2) The utility model also comprises a handle, the handle is a telescopic handle, the length of the handle can be adjusted according to different test tubes, and personnel can conveniently hold and operate the handle.

[0018] In the utility model, the above technical schemes can be combined with each other to realize more optional combination schemes. Other features and advantages of the utility model will be described in the subsequent description, and some advantages can be apparent from the description or can be understood by implementing the utility model. The purpose and other advantages of the utility model can be realized and obtained by the contents specially pointed out in the description and the drawings. BRIEF DESCRIPTION OF DRAWINGS

[0019] The drawings are only used for the purpose of showing specific embodiments and are not considered as limiting the utility model, and the same reference signs represent the same parts throughout the drawings.

[0020] Figure 1 It is a structure schematic view of the microfluidic sperm optimization device of the utility model embodiment.

[0021] Figure 2 It is a structure schematic view of the microfluidic sperm optimization device of the utility model embodiment.

[0022] Figure 3 It is a sectional view of the microfluidic sperm optimization device of the utility model embodiment.

[0023] Reference signs:

[0024] 1, handle; 2, collecting pool; 3, screening element; 31, screening channel; 32, stepped contact surface; 321, first stepped contact surface; 322, second stepped contact surface. DETAILED DESCRIPTION

[0025] The preferred embodiments of the utility model will be described in detail below with reference to the drawings, wherein the drawings form a part of the utility model and are used together with the embodiments of the utility model to explain the principles of the utility model, but are not used to limit the scope of the utility model.

[0026] One specific embodiment of the utility model discloses a microfluidic sperm optimal selection device, as shown in Figure 1 and Figure 2 , which comprises a collecting pool 2 and a screening piece 3, the screening piece 3 is connected with the collecting pool 2, the screening piece 3 can be soaked into raw semen, is used for simulating the oviduct to screen the high-quality sperm in the raw semen into the collecting pool 2, and the collecting pool 2 is used for collecting the screened high-quality sperm; compared with the prior art, the embodiment can simulate the oviduct by soaking the screening piece 3 into the raw semen, can screen the high-quality sperm in the raw semen into the collecting pool 2 by using the boundary tracking navigation ability of sperm, is simple in operation, and the screening process does not need to be centrifuged, so that the sperm DNA is not damaged and broken by peroxidation, the concentration of the screened high-quality sperm is improved, and then the fertilization rate is improved.

[0027] Preferably, as shown in Figure 3 , the screening piece 3 is provided with a screening channel 31, the screening channel 31 has an inlet end and an outlet end, the inlet end can be soaked into raw semen, the outlet end is connected with the collecting pool 2, the screening channel 31 is used for simulating the oviduct, and the boundary tracking navigation ability of sperm is used for screening sperm, so that the operation is simple, the screening process does not need to be centrifuged, the sperm DNA is not damaged and broken by peroxidation, the concentration of the screened high-quality sperm is improved, and then the fertilization rate is improved.

[0028] Preferably, the outlet end of the screening channel 31 gradually reduces in diameter from the inlet end to the outlet end in the direction, so that the high-quality sperm screened into the collecting pool 2 is prevented from swimming into the screening channel 31, the screened high-quality sperm is prevented from swimming into the raw semen, the concentration of the screened high-quality sperm is further improved.

[0029] Preferably, a plurality of screening channels 31 are arranged, the plurality of screening channels 31 provide a plurality of paths for sperm swimming from the raw semen into the collecting pool 2, so that the screening efficiency of high-quality sperm is improved.

[0030] Preferably, the screening piece 3 is provided with a stepped contact surface 32 on the side soaked with raw semen, the stepped contact surface 32 can increase the contact surface of the screening piece 3 and raw semen, so that the inlet end of the screening channel 31 can be fully exposed in the raw semen, and then the active sperm can be maximized to swim to the collecting pool through the screening channel 31, so that the screening efficiency is further improved.

[0031] Preferably, the stepped contact surface 32 comprises a first stepped contact surface 321 located away from the axis of the screening member 3, which can adapt to the arc surface of the bottom of the test tube, so as to expand the contact area of the screening member 3 with the raw semen, so that the inlet end of the screening channel 31 away from the axis of the screening member 3 can be fully exposed to the raw semen at the edge of the test tube; the second stepped contact surface 322 is located close to the axis of the screening member 3, so that the inlet end of the screening channel 31 close to the axis of the screening member 3 can be fully exposed to the raw semen at the middle of the test tube; thereby maximizing the guidance of the motile sperm to swim through the screening channel 31 to the collection pool, so as to further improve the screening efficiency.

[0032] Preferably, the screening channel 31 is a capillary channel.

[0033] Preferably, the microfluidic sperm concentrator further comprises a handle 1 installed in the collection pool 2, which facilitates the personnel to hold and operate.

[0034] Preferably, the handle 1 is a telescopic handle, which can adjust the length of the handle 1 according to different test tubes, further facilitating the personnel to hold and operate.

[0035] Preferably, the handle 1 is a hollow handle, which facilitates the discharge of bubbles and makes the raw semen contact with the screening channel 31.

[0036] In use, the microfluidic sperm concentrator is immersed in the G-IVF liquid for assisted reproduction for 10 minutes, so that the inside of the screening channel 31 is filled with the G-IVF liquid through capillary action; the screening member 3 is immersed in the test tube containing unprocessed raw semen; 1 mL of G-IVF liquid is added to the collection pool 2; incubated at 37℃ for 30 minutes, so that the motile sperm swim through the screening channel 31 to the collection pool 2; the sorted sperm is collected by a pipette.

[0037] The above is only a preferred specific embodiment of the present application, but the protection scope of the present application is not limited thereto, any person skilled in the art can easily think of changes or replacements within the technical range disclosed by the present application, which should be covered within the protection scope of the present application.

Claims

1. A microfluidic sperm preferencer, characterized in that, The device comprises a collecting pool (2) and a screening member (3) connected with the collecting pool (2), the screening member (3) is provided with a screening channel (31), the screening channel (31) has an inlet end and an outlet end, the inlet end can be immersed in the raw semen, and the outlet end is connected with the collecting pool (2); the screening channel (31) simulates the oviduct, and sperm screening is realized by using the boundary tracking navigation ability of sperm. The diameter of the outlet end of the screening channel (31) gradually decreases, so that the high-quality sperm screened into the collecting pool (2) can be prevented from swimming into the screening channel (31).

2. Microfluidic sperm preferencer according to claim 1, characterized in that The screening member (3) is provided with a stepped contact surface, and the stepped contact surface can increase the contact surface between the screening member (3) and the raw semen.

3. Microfluidic sperm preferencer according to claim 2, characterized in that The stepped contact surface (32) comprises a first stepped contact surface (321) and a second stepped contact surface (322).

4. The microfluidic sperm preferencer according to claim 3, characterized in that, The first stepped contact surface (321) is located away from the axis of the screening member (3).

5. The microfluidic sperm preferencer according to claim 4, characterized in that, The second stepped contact surface (322) is located close to the axis of the screening member (3).

6. The microfluidic sperm preferencer according to claim 5, characterized in that, The screening channel (31) is a capillary channel.

7. The microfluidic sperm preferencer according to claim 6, characterized in that The screening channel (31) is provided with a plurality of screening channels.

8. Microfluidic sperm preferencer according to claim 7, characterized in that The device further comprises a handle (1).

9. Microfluidic sperm preferencer according to claim 8, characterized in that The handle (1) is installed in the collecting pool (2).

10. The microfluidic sperm preferencer according to claim 9, characterized in that The handle (1) is a telescopic handle.

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