Fluorescent quantitative PCR automatic all-in-one machine suitable for rapid detection of animal and plant pests

Abstract

The utility model relates to the technical field of nucleic acid extraction, in particular to a fluorescent quantitative PCR (Polymerase Chain Reaction) automatic all-in-one machine suitable for rapidly detecting animal and plant pests, which comprises a machine table, a fluorescent quantitative PCR detection device, a fluorescent quantitative PCR detection device and a fluorescent quantitative PCR detection device, and is characterized in that the machine table comprises a bottom plate and a support frame arranged on the bottom plate; a refrigeration module, a grinding instrument, a centrifugal machine, a magnetic frame and a PCR instrument are sequentially arranged from one end to the other end of the bottom plate; the carrying mechanism comprises a carrying arm and a clamping jaw rotationally arranged on the carrying arm, the carrying arm is arranged on the supporting frame and can move between the refrigeration module and the PCR instrument, and the clamping jaw is used for carrying a grinding pipe. According to the scheme, through integration of multifunctional modules and configuration of an automatic carrying structure, the automation degree and the processing efficiency of sample processing are remarkably improved, space utilization and process layout are optimized, the application range is expanded, and errors and pollution risks are effectively reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to nucleic acid extraction technical field more specifically relates to a kind of fluorescence quantitative PCR automation integrated machine suitable for rapid detection of animal and plant harmful organisms. BACKGROUND

[0002] Molecular biological technology as one of the most advanced experimental methods in modern biological technology, has been widely used in clinical detection, basic medical research, drug development, epidemic prevention and control and food safety and other fields. At present, commonly used molecular biological technology includes nucleic acid extraction, gene sequencing, polymerase chain reaction (PCR) and biochip etc. These technologies involve sample processing, storage, reagent distribution, gradient dilution, micropipetting and purification and other complex and precise liquid operations, with extremely high requirements for operation precision and repeatability.

[0003] In the prior art, sample liquid processing usually relies on liquid workstations. However, the liquid workstations on the domestic market are mainly imported brands, which are expensive and not well suited to domestic specific applications, have poor compatibility and insufficient product targeting. The existing general liquid workstations are mainly used for pre-processing of molecular diagnostic platforms, such as pipetting, plate transfer and oscillation, and their system functions are relatively simple, and they cannot complete the complete nucleic acid detection process.

[0004] In view of this, the applicant actively carries out improvement research in view of the many shortcomings of existing liquid workstations, and finally designs and develops the utility model to meet the more efficient and accurate molecular biological liquid processing needs. UTILITY MODEL CONTENT

[0005] Therefore, the utility model provides a fluorescence quantitative PCR automation integrated machine suitable for rapid detection of animal and plant harmful organisms, which can be combined and disassembled, suitable for different scenarios and costs, realizes high-throughput micro nucleic acid extraction function, and can be used as a general liquid workstation for pipetting and other biological experiments.

[0006] To achieve the above purpose, the utility model provides the following technical scheme: a fluorescence quantitative PCR automation integrated machine suitable for rapid detection of animal and plant harmful organisms, comprising:

[0007] A machine table, the machine table includes a bottom plate and a support frame arranged on the bottom plate, and a refrigeration module, a grinder, a centrifuge, a magnetic stand and a PCR instrument are arranged on the bottom plate in sequence from one end to the other end according to the sample extraction process.

[0008] A carrying mechanism, comprising a carrying arm and a gripper rotatably arranged on the carrying arm, the carrying arm is arranged on the support frame and can move between the refrigeration module and the PCR instrument, and the gripper is used for carrying the grinding tube.

[0009] Preferably, in the above-mentioned fluorescent quantitative PCR automatic integrated machine suitable for rapid detection of animal and plant harmful organisms, the support frame has a first direction, a second direction and a third direction perpendicular to each other, and the three directions are directions in which the carrying arm approaches or moves away from the bottom plate.

[0010] The carrying arm can move along the first direction, the second direction and the third direction on the support frame.

[0011] Preferably, in the above-mentioned fluorescent quantitative PCR automatic integrated machine suitable for rapid detection of animal and plant harmful organisms, a sample tube rack and a magnetic rack are arranged in the middle of the bottom plate, and a PCR plate and a deep well plate are arranged outside the sample tube rack and the magnetic rack; a gun head box is arranged outside the refrigeration module.

[0012] Preferably, in the above-mentioned fluorescent quantitative PCR automatic integrated machine suitable for rapid detection of animal and plant harmful organisms, a heating and shaking instrument is arranged between the refrigeration module and the gun head box.

[0013] Preferably, in the above-mentioned fluorescent quantitative PCR automatic integrated machine suitable for rapid detection of animal and plant harmful organisms, the oscillation module comprises a support plate, a heat-conducting block arranged on the bottom of the support plate and provided with a heating sheet, and an eccentric oscillation assembly for driving the support plate to eccentrically vibrate.

[0014] According to the above technical solution, compared with the prior art, the fluorescent quantitative PCR automatic integrated machine suitable for rapid detection of animal and plant harmful organisms has the following advantages:

[0015] 1. The liquid workstation integrates multiple functional units such as a refrigeration module, a grinding instrument, a centrifuge, a magnetic rack and a PCR instrument, which are usually dispersed in different devices, on the same bottom plate and frame structure; without transferring samples between different devices, the operation steps and device occupation space are reduced; the continuity and compactness of the overall experimental process are improved, the experimental period is shortened, and the error and pollution risk caused by multiple sample transfers are reduced.

[0016] 2. The design of the transport arm and its grippers allows for flexible transfer of samples (e.g., grinding tubes) between the cooling module and the PCR instrument; reduces manual intervention, improves productivity and reproducibility; enhances the automation level of the system, enabling 24-hour uninterrupted high-throughput operation; significantly reduces the workload of laboratory operators and human error, achieving higher sample processing accuracy and stability.

[0017] 3. Sample tube racks and magnetic racks are set in the middle of the base plate, and PCR plates, deep well plates, heated shakers, pipette tip boxes and cooling modules are arranged reasonably on the outside of them; the compact and reasonable layout reduces the handling path and improves the efficiency and accuracy of the handling arm when performing pick-up and put-down operations; it helps to improve space utilization and can integrate more functions in a limited laboratory space.

[0018] The heated shaker positioned between the cooling module and the pipette tip box, along with the heating element and eccentric oscillation assembly within the oscillation module, allows this workstation to perform not only cooling, grinding, centrifugation, and PCR, but also heating and thorough oscillation mixing of samples. This meets the temperature control and mixing requirements for more types of sample pretreatment, expanding the instrument's applicable experimental scope. By utilizing the heating element and eccentric oscillation assembly to achieve heating and oscillation within the same module, switching between complex devices is reduced, improving the efficiency and accuracy of experimental processing. Through temperature control and oscillation mixing, sample reaction conditions are improved, enhancing the reproducibility and reliability of experimental data. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0020] Figure 1 The attached figure is a schematic diagram of the structure of this utility model.

[0021] In the attached diagram: 1-base plate; 2-support frame; 3-cooling module; 4-grinder; 5-centrifuge; 6-magnetic rack; 7-PCR instrument; 8-transfer arm; 9-gripper; 10-sample tube rack; 11-PCR plate; 12-deep well plate; 13-pipe tip box; 14-heating shaker. Detailed Implementation

[0022] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the utility model.

[0023] Referring to Figure 1 A fluorescence quantitative PCR automatic integrated machine suitable for rapid detection of animal and plant harmful organisms comprises a machine table and a plurality of function modules and a carrying mechanism arranged on the machine table, and is used for automatically performing a series of operations such as refrigeration, grinding, centrifugation, magnetic separation and amplification on biological samples.

[0024] The machine table comprises a bottom plate 1 and a support frame 2 arranged on the bottom plate 1. The support frame 2 is a frame structure with multidirectional guide rails or guide mechanisms, which forms a support system for multi-axis movement of a carrying arm. From one end to the other end of the bottom plate 1, a refrigeration module 3, a grinder 4, a centrifuge 5, a magnetic stand 6 and a PCR instrument 7 are sequentially arranged according to a predetermined process flow sequence of sample extraction and processing. This arrangement can be customized according to the experimental steps of the user to realize the sequential flow and processing of the sample.

[0025] In the embodiment, the refrigeration module 3 is used for low-temperature preservation or preliminary cooling of the to-be-processed sample to prevent degradation of the sample in the subsequent processing steps. The grinder 4 can crush and homogenize the biological tissue sample to release target substances such as nucleic acids. The centrifuge 5 can separate the treated solution by high-speed rotation, and the magnetic stand 6 can separate and purify nucleic acids by using magnetic bead technology. Finally, the PCR instrument 7 is used for amplifying the obtained nucleic acids to provide sufficient nucleic acid templates for subsequent analysis.

[0026] The support frame 2 is provided with three groups of perpendicular guide rail structures, which are defined as a first direction, a second direction and a third direction, and the third direction is the up-down direction (Z direction) of the carrying arm perpendicular to the plane of the bottom plate 1. In this way, a carrying mechanism capable of multi-axis movement is installed on the support frame 2. The carrying mechanism comprises a carrying arm 8 and a gripper 9 rotatably arranged at the end of the carrying arm 8. Through the guide rails of the support frame 2, the carrying arm 8 can move in the first direction (X direction), the second direction (Y direction) and the third direction (Z direction), so as to freely move in the plane range and the vertical direction of the entire bottom plate 1.

[0027] In actual operation, when the sample needs to be processed, the operator or the control system will give an instruction, the handling arm 8 will move along the guide rail of the support frame 2, and the sample tube containing the sample will be clamped from the refrigeration module 3 by the clamping jaw 9, and then sent to the grinder 4 for grinding processing, and then the sample tube after grinding is transferred to the centrifuge 5, the magnetic stand 6 and the PCR instrument 7 for step-by-step processing. The whole process realizes continuous and automatic operation, and the sample tube does not need to be manually transferred between devices, thereby improving the operation efficiency and reducing the human error and pollution risk.

[0028] To further optimize the layout of each processing and storage unit on the bottom plate 1, the sample tube rack 10 and the magnetic stand 6 are arranged at the middle position of the bottom plate 1 in this embodiment, and the PCR plate 11 and the deep well plate 12 are arranged around the outside of the sample tube rack 10 and the magnetic stand 6. Through this relatively concentrated arrangement, the path of the handling arm 8 is more compact when the sample is grabbed and placed, and unnecessary back-and-forth distance is reduced. Meanwhile, the gun head box 13 is arranged in the outer side area of the refrigeration module 3, so that the handling arm 8 can directly obtain the pipette tip nearby when it needs to be replaced.

[0029] The space between the refrigeration module 3 and the gun head box 13 is provided with a heating and shaking instrument 14. The heating and shaking instrument 14 is used as a supplementary module to heat and shake the sample. Through reasonable planning of the layout on the bottom plate 1, the heating and shaking steps can be flexibly inserted in the middle of the sample processing flow, so as to meet the demand of sample heating and mixing for specific experiments, thereby expanding the application range of the liquid workstation.

[0030] The specific structure of the heating and shaking instrument 14 further comprises a shaking module. The shaking module comprises a support plate, a heat-conducting block and an eccentric shaking assembly, wherein the heat-conducting block is provided with a heating sheet at the bottom, which is used to provide temperature control for the sample to be processed. The eccentric shaking assembly can drive the support plate to produce eccentric vibration, so as to drive the sample in the micro-hole plate, test tube or other container placed thereon to be fully mixed. When the heating and shaking operation is needed, the handling arm 8 places the target sample tube or plate on the heating and shaking instrument 14 through the clamping jaw 9, and sets the appropriate heating temperature and shaking frequency through the control system to quickly and uniformly mix the sample. Due to the close combination of the heating sheet and the eccentric shaking assembly, the device does not need to be transferred to a separate heating or shaking device in steps, which greatly simplifies the processing flow.

[0031] It can be seen from the introduction of the above embodiments that the fluorescence quantitative PCR automatic integrated machine suitable for rapid detection of animal and plant harmful organisms integrates multiple key processing modules on the same machine, and is matched with a multi-axis free moving carrying arm and a clamping jaw, so that full automation linkage from sample pretreatment (refrigeration, grinding, centrifugation, magnetic separation) to nucleic acid amplification (PCR) is realized. At the same time, through reasonable layout of the sample tube rack, the magnetic rack, the PCR plate, the deep hole plate, the gun head box and the heating oscillator, the whole process path is simplified, the equipment occupies less space, and the operation accuracy and repeatability are improved. The embodiment can be widely applied to the fields of molecular biology detection, medical diagnosis, high-throughput screening and related biochemical analysis, and provides a user with an efficient, stable and full-automatic sample processing solution.

[0032] The various embodiments in the specification are described in a progressive manner, and each embodiment focuses on the difference from other embodiments, and the same or similar parts between the various embodiments can be referred to each other. For the device disclosed by the embodiments, since it corresponds to the method disclosed by the embodiments, the description is relatively simple, and the related parts can be referred to the method part.

[0033] The above description of the disclosed embodiments enables a person skilled in the art to implement or use the present application. Various modifications to the embodiments will be apparent to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A fluorescence quantitative PCR automatic integrated machine suitable for rapid detection of animal and plant pests, characterized in that, The application relates to a sample processing platform. The platform comprises a base plate and a support frame arranged on the base plate, wherein a refrigeration module, a grinder, a centrifuge, a magnetic stand and a PCR instrument are sequentially arranged on the base plate from one end to the other end according to a sample extraction process. The carrying mechanism comprises a carrying arm and a gripper rotatably arranged on the carrying arm, wherein the carrying arm is arranged on the support frame and can move between the refrigeration module and the PCR instrument, and the gripper is used for carrying a grinding tube.

2. The automated kiosk of claim 1, wherein, The support frame has a first direction, a second direction and a third direction which are perpendicular to each other, and the three directions are directions in which the carrying arm approaches or moves away from the base plate. The carrying arm can move along the first direction, the second direction and the third direction on the support frame.

3. The automated kiosk of claim 1, wherein, A sample tube rack and a magnetic stand are arranged in the middle of the base plate, and a PCR plate and a deep hole plate are arranged outside the sample tube rack and the magnetic stand; a gun head box is arranged outside the refrigeration module.

4. The automated kiosk of claim 3, wherein, A heating oscillator is arranged between the refrigeration module and the gun head box.

5. The automated unit of claim 4, wherein, The application further relates to an oscillation module which comprises a support plate, heat-conducting blocks arranged on the support plate and having heating sheets arranged at the bottom, and an eccentric oscillation assembly used for driving the support plate to eccentrically oscillate.

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