PCR kit
By introducing an ice chamber and circulation module into the PCR kit, combined with a vacuum insulation layer and a sensing module, the function of low-temperature preservation and transportation of PCR reagents is realized, solving the problem that existing technologies cannot preserve reagents at low temperatures, and improving the insulation effect and convenience of the kit.
Patent Information
- Application Number
- CN202520034091.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-08
- Publication Date
- 2026-01-02
- Estimated Expiration
- 2035-01-08
AI Technical Summary
Existing PCR kits cannot achieve low-temperature preservation and transportation of PCR tubes, and cannot meet the needs of special application scenarios.
A PCR kit was designed, comprising a box, a removable cap, a carrier, an ice chamber, and a circulation module. Through the cooperation of a sensing module and a switch button, an air pump is automatically activated to circulate the cold air in the ice chamber to the top of the carrier, providing a low-temperature environment. The heat preservation effect is improved through a vacuum insulation layer and a heat insulation coating.
This technology enables the cryopreservation and transportation of PCR reagents, ensuring that the reagents remain in a low-temperature environment during transportation, thereby improving the insulation effect and ease of use of the kits.
Smart Images
Figure CN223752803U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the field of biological experimental consumables, in particular to a PCR kit. BACKGROUND
[0002] Polymerase Chain Reaction (PCR) is a commonly used technique in molecular biology for amplifying DNA fragments. PCR can rapidly amplify specific DNA sequences in vitro, exponentially increasing their number, providing an important tool for gene detection, molecular cloning, sequence analysis, etc.
[0003] PCR technology has a wide range of applications in molecular biology, medical diagnosis, forensic science, genetics, etc., such as in gene cloning, gene detection, disease diagnosis, DNA fingerprint identification, etc. The high sensitivity and specificity of PCR technology make it an important tool for many biological and medical researches.
[0004] In the prior art, the common reagent kit mainly includes a box body, a carrier is fixedly installed in the box body, a plurality of placing tubes are fixedly installed at the bottom of the carrier, a plurality of insertion holes for inserting PCR tubes are formed on the carrier, and the insertion holes are located at the tube openings of the placing tubes.
[0005] However, in some special application scenarios, PCR tubes need to be stored and transported at low temperature, and the existing PCR reagent kit cannot achieve the effect of storing and transporting PCR tubes at low temperature, and there is room for improvement. CONTENT OF THE INVENTION
[0006] In order to facilitate the user to store and transport the reagent which needs to be stored at low temperature, the present application provides a PCR kit.
[0007] The PCR kit provided by the present application adopts the following technical scheme:
[0008] A PCR kit includes a box body, a cover is detachably installed on the top of the box body, a carrier is detachably installed in the box body, a plurality of placing tubes for placing PCR tubes are fixedly installed at the bottom of the carrier, and a plurality of insertion holes for the PCR tubes to extend into the placing tubes are formed through the carrier.
[0009] A plurality of ice block chambers for placing ice blocks are formed below the placing tubes, and
[0010] A switch button is fixedly installed on the outer side wall of the box body for inputting a start signal.
[0011] An inductive module is arranged on the box body and connected with the signal output end of the switch button, for receiving the starting signal and outputting a trigger signal when the cover is completely installed;
[0012] A circulating module is arranged in the ice block chamber and connected with the signal output end of the inductive module, for receiving the trigger signal and circulating the cold air in the ice block chamber to the top of the carrier.
[0013] By using the above technical scheme, when the PCR kit is needed, the operator can take out the carrier in advance, put the ice block at the bottom of the box body, then reinstall the carrier in the box body, and start the switch button outside the box body; after the operator installs the cover on the box body, the inductive module outputs the trigger signal, and the circulating module receives the trigger signal to drive the cold air in the ice block chamber to circulate to the top of the carrier, so that the temperature in the box body circulates up and down, and a low-temperature environment can be provided for the reagent in the PCR tube.
[0014] Preferably, the box body comprises an inner container and an outer shell, and a heat insulation vacuum layer is formed between the inner container and the outer shell.
[0015] By using the above technical scheme, the vacuum heat insulation layer can form a good heat insulation environment, and the heat preservation effect of the reagent kit is improved.
[0016] Preferably, the outer side wall of the inner container and the inner side wall of the outer shell are coated with a heat insulation coating.
[0017] By using the above technical scheme, the heat insulation coating can further improve the heat preservation effect of the reagent kit.
[0018] Preferably, a threaded connection part is integrally formed at the opening of the box body, an internal thread is formed on the cover, and the cover is threadedly connected to the threaded connection part.
[0019] By using the above technical scheme, the cover can be detachably connected to the box body through threaded connection.
[0020] Preferably, an annular table is formed on the side wall of the outer shell, and the inductive module comprises:
[0021] A thin film pressure sensor is fixedly arranged on the annular table, for detecting the pressure between the bottom of the cover and outputting an inductive pressure signal;
[0022] A comparator chip is connected with the signal output end of the thin film pressure sensor, for receiving the inductive pressure signal and outputting the trigger signal when the inductive pressure is greater than a set value;
[0023] The signal output end of the comparator chip is connected with the signal input end of the circulating module.
[0024] By adopting the above technical scheme, when the operator inputs the starting signal through the switch button, the thin film pressure sensor can detect the contact pressure between the bottom of the cover and the annular table in real time. When the cover is completely installed on the box body, the bottom of the cover abuts against the annular table, and the inductive pressure value detected by the thin film pressure sensor is greater than the set pressure value, the comparator chip outputs a trigger signal.
[0025] Preferably, the circulating module comprises a gas pump fixedly installed on the inner wall of the inner container, an air outlet pipeline is arranged in communication with the air outlet end of the gas pump, arc-shaped notches are formed on the two sides of the carrier, and the signal input ends of the two gas pumps are connected with the signal output end of the comparator chip.
[0026] By adopting the above technical scheme, when the cover is completely installed on the box body and the comparator chip outputs a trigger signal, the gas pump receives the trigger signal and operates, continuously conveying the cold air in the ice block chamber to the upper side of the carrier, so that the cold air in the box body is circulated, and the low-temperature environment in the box body is more uniform.
[0027] Preferably, a plurality of connecting seats are fixedly installed on the inner wall of the inner container, a connecting hole is formed in communication through each of the connecting seats, a plurality of insertion rods are fixedly installed on the bottom of the carrier, and the insertion rods are inserted into the connecting holes, respectively.
[0028] By adopting the above technical scheme, the carrier can be detachably installed in the box body through the connecting holes in the connecting seats and the insertion rods on the bottom of the carrier.
[0029] Preferably, a handheld handle is fixedly installed on the top of the cover.
[0030] By adopting the above technical scheme, the handheld handle can facilitate the operator to transfer and carry the reagent box, and improve the use convenience of the reagent box.
[0031] In summary, the PCR reagent box has at least one of the following beneficial technical effects:
[0032] 1. When the cover is completely installed on the box body and the comparator chip outputs a trigger signal, the gas pump receives the trigger signal and operates, continuously conveying the cold air in the ice block chamber to the upper side of the carrier, so that the cold air in the box body is circulated, and the low-temperature environment in the box body is more uniform.
[0033] 2. The thin film pressure sensor and the comparator chip can be matched and used with each other to automatically sense the installation state of the cover and automatically start the gas pump. BRIEF DESCRIPTION OF DRAWINGS
[0034] Figure 1 is a schematic diagram for showing the overall structure of the kit according to the embodiment of the present application.
[0035] Figure 2 is a schematic diagram for showing the internal structure of the box according to the embodiment of the present application.
[0036] Mark explanation: 1, box; 11, ice block chamber; 12, switch button; 13, inner container; 14, outer shell; 15, heat insulation vacuum layer; 16, threaded connection part; 17, annular table top; 18, connecting seat; 2, cover; 21, handheld handle; 3, carrier; 31, placement tube; 32, insertion hole; 33, insertion rod; 34, arc-shaped notch; 4, thin film pressure sensor; 5, air pump; 51, air outlet pipeline. DETAILED DESCRIPTION
[0037] The following will be described in detail in combination with the accompanying Figures 1-2 The present application is further described in detail.
[0038] EMBODIMENT
[0039] The present application discloses a PCR kit. Referring to Figure 1 With Figure 2 , including box 1, the top of the box 1 is detachably mounted with cover 2, the box 1 is detachably mounted with carrier 3, the bottom of the carrier 3 is fixedly mounted with a plurality of placement tubes 31 for placing PCR tubes, a plurality of insertion holes 32 are provided through the carrier 3 for the PCR tubes to extend into the placement tubes 31.
[0040] Referring to Figure 1 , two oppositely arranged connecting seats 18 are fixedly mounted on the inner wall of the inner container 13, connecting holes are provided through the connecting seats 18, respectively, two insertion rods 33 are fixedly mounted on the bottom of the carrier 3, and the insertion rods 33 are respectively inserted into the connecting holes.
[0041] Through the connecting holes on the connecting seats 18 and the insertion rods 33 on the bottom of the carrier 3, the technical effect that the carrier 3 can be detachably mounted in the box 1 can be achieved.
[0042] A plurality of ice block chambers 11 are formed below the plurality of placement tubes 31 for placing ice blocks, and: a switch button 12 is fixedly mounted on the outer side wall of the box 1 for inputting a start signal; a sensing module is mounted on the box 1 and is signal connected with the signal output end of the switch button 12 for receiving the start signal and outputting a trigger signal when the cover 2 is completely mounted; a circulating module is mounted in the ice block chamber 11 and is signal connected with the signal output end of the sensing module for receiving the trigger signal and circulating the cold air in the ice block chamber 11 to the upper side of the carrier 3.
[0043] When the PCR kit needs to be used, the operator can take out the carrier 3 in advance, put ice blocks at the bottom of the box body 1, then re-install the carrier 3 in the box body 1, and start the switch button 12 outside the box body 1; after the operator installs the cover 2 on the box body 1, the induction module outputs a trigger signal, and after the cycle module receives the trigger signal, the cold air in the ice block chamber 11 is circulated to the upper side of the carrier 3, so that the temperature in the box body 1 circulates up and down, and a low-temperature environment can be provided for the reagent in the PCR tube.
[0044] With reference to Figure 1 , the box body 1 comprises an inner container 13 and an outer shell 14, and a heat insulation vacuum layer 15 is formed between the inner container 13 and the outer shell 14. The vacuum heat insulation layer can form a good heat insulation environment and improve the heat preservation effect of the reagent kit.
[0045] Further, the outer side wall of the inner container 13 and the inner side wall of the outer shell 14 are coated with a heat insulation coating. The heat insulation coating can further improve the heat preservation effect of the reagent kit.
[0046] In the embodiment of the application, the opening of the box body 1 is integrally formed with a threaded connection part 16, the cover 2 is formed with an internal thread, and the cover 2 is threadedly connected to the threaded connection part 16. The cover 2 can be detachably connected to the box body 1 by threadedly connecting the cover 2 to the box body 1.
[0047] With reference to Figure 1 and Figure 2 , the sidewall of the outer shell 14 is formed with an annular table 17, and the induction module comprises: a thin film pressure sensor 4 fixedly installed on the annular table 17, used for detecting the pressure between the bottom of the cover 2 and the annular table 17 and outputting an induction pressure signal; a comparator chip, a signal input end of which is signal connected with a signal output end of the thin film pressure sensor 4, used for receiving the induction pressure signal and outputting a trigger signal when the induction pressure is greater than a set value; and a signal output end of the comparator chip is signal connected with a signal input end of the cycle module.
[0048] When the operator inputs a start signal through the switch button 12, the thin film pressure sensor 4 can detect the contact pressure between the bottom of the cover 2 and the annular table 17 in real time. When the cover 2 is completely installed on the box body 1 and the bottom of the cover 2 abuts against the annular table 17, the induction pressure value detected by the thin film pressure sensor 4 is greater than the set pressure value, and the comparator chip outputs a trigger signal.
[0049] The cycle module comprises a gas pump 5 fixedly installed on the inner wall of the inner container 13, the gas outlet end of the gas pump 5 is communicatively provided with a gas outlet pipeline 51, arc-shaped notches 34 are formed on the two sides of the carrier 3, and the signal input ends of the two gas pumps 5 are signal connected with the signal output end of the comparator chip.
[0050] It needs to be explained that in the embodiment of the application, the mounting seat is fixedly installed on the inner wall of the inner container 13 by welding, the number of the air pumps 5 is two, and the two air pumps 5 are fixed on the mounting seat by gluing respectively, and the air outlet pipeline 51 communicated on the two air pumps 5 is fixed on the inner wall of the inner container 13 by gluing.
[0051] When the cover 2 is completely installed on the box body 1, and the comparator chip outputs a trigger signal, the air pump 5 receives the trigger signal and operates, and continuously transports the cold air in the ice block chamber 11 to the upper side of the carrier 3, so that the cold air in the box body 1 circulates, and the low-temperature environment in the box body 1 is more uniform.
[0052] Through the setting of the arc-shaped notches 34 on the two sides of the carrier 3, the user can hold the carrier 3 by both hands during the process of taking and placing the carrier 3.
[0053] When the user opens the cover 2 on the reagent box and takes out the carrier 3, the user can press the switch button 12 again, so that the thin film pressure sensor 4 stops working.
[0054] Reference Figure 1 The top of the cover 2 is fixedly installed with a handheld handle 21. The setting of the handheld handle 21 can facilitate the operator to transfer and carry the reagent box, and improve the use convenience of the reagent box.
[0055] The implementation principle of the PCR reagent box in the embodiment of the application is as follows: when the PCR reagent box needs to be used, the operator can take out the carrier 3 in advance, put ice blocks at the bottom of the box body 1, then reinstall the carrier 3 in the box body 1, and start the switch button 12 on the outer side of the box body 1; when the operator installs the cover 2 on the box body 1, the bottom of the cover 2 abuts against the annular table top 17, the sensing pressure measured by the thin film pressure sensor 4 on the annular table top 17 increases to exceed the set pressure value, the comparator chip outputs a trigger signal, the air pump 5 receives the trigger signal and operates, and can drive the cold air in the ice block chamber 11 to circulate and flow to the upper side of the carrier 3, so that the temperature in the box body 1 circulates and flows up and down, and the reagent in the PCR tube can be provided with a low-temperature environment, which is convenient for the user to store and transport the reagent which needs to be stored at low temperature.
[0056] The above are the preferred embodiments of the application, and do not limit the protection scope of the application, so: any equivalent changes made according to the structure, shape and principle of the application should be covered in the protection scope of the application.
Claims
1. A PCR kit, characterized by, The utility model relates to a PCR (Polymerase Chain Reaction) box, comprising a box body (1), a cover (2) detachably mounted on the top of the box body (1), and a carrier (3) detachably mounted in the box body (1), wherein the bottom of the carrier (3) is fixedly mounted with a plurality of placing tubes (31) for placing PCR tubes, and a plurality of insertion holes (32) are formed through the carrier (3) for the PCR tubes to extend into the placing tubes (31); a plurality of ice block chambers (11) are formed below the placing tubes (31) for placing ice blocks, and a switch button (12) is fixedly mounted on the outer sidewall of the box body (1) for inputting a start signal; an induction module is mounted on the box body (1) and connected to the signal output end of the switch button (12) for receiving the start signal and outputting a trigger signal when the cover (2) is completely mounted; a circulation module is mounted in the ice block chamber (11) and connected to the signal output end of the induction module for receiving the trigger signal and circulating the cold air in the ice block chamber (11) to the top of the carrier (3).
2. The PCR kit according to claim 1, characterized in that, The box body (1) comprises an inner container (13) and an outer shell (14), and a heat insulation vacuum layer (15) is formed between the inner container (13) and the outer shell (14).
3. The PCR kit according to claim 2, characterized in that, The outer sidewall of the inner container (13) and the inner sidewall of the outer shell (14) are coated with a heat insulation coating.
4. The PCR kit according to claim 1, characterized in that, A threaded connection part (16) is integrally formed at the opening of the box body (1), an inner thread is formed on the cover (2), and the cover (2) is threadedly connected to the threaded connection part (16).
5. The PCR kit according to claim 2, wherein An annular table (17) is formed on the sidewall of the outer shell (14), and the induction module comprises: a thin film pressure sensor (4) is fixedly mounted on the annular table (17) for detecting the pressure between the bottom of the cover (2) and outputting an induction pressure signal; a comparator chip is connected to the signal output end of the thin film pressure sensor (4) for receiving the induction pressure signal and outputting the trigger signal when the induction pressure is greater than a set value; the signal output end of the comparator chip is connected to the signal input end of the circulation module.
6. The PCR kit according to claim 5, characterized in that, The circulation module comprises a gas pump (5) fixedly mounted on the inner wall of the inner container (13), an air outlet pipe (51) is connected to the air outlet end of the gas pump (5), arc-shaped notches (34) are formed on both sides of the carrier (3), and the signal input ends of the two gas pumps (5) are connected to the signal output end of the comparator chip.
7. The PCR kit according to claim 6, characterized in that, A plurality of connecting seats (18) are fixedly mounted on the inner wall of the inner container (13), a connecting hole is formed through each of the connecting seats (18), and a plurality of insertion rods (33) are fixedly mounted on the bottom of the carrier (3) and inserted into the connecting holes.
8. The PCR kit according to claim 1, wherein A handgrip (21) is fixedly mounted on the top of the cover (2).