Cell disruption device

Abstract

The utility model belongs to the technical field of cell disruption, and particularly relates to a cell disruption device which comprises a disruption barrel, a barrel cover arranged on the disruption barrel, a disruption mechanism and a longitudinal disturbance mechanism for longitudinally disturbing liquid in the disruption barrel, the longitudinal disturbance mechanism comprises a disturbance plate arranged between the crushing mechanism and the bottom of the crushing barrel and a disturbance driving device used for driving the disturbance plate to do longitudinal reciprocating motion. Longitudinal through circulation holes are formed in the disturbance plate in a scattered mode. The cell crushing device is used for solving the technical problem that the cell crushing rate is low due to the fact that an existing crushing device has small disturbance on a solution.

Description

Technical Field

[0001] This invention belongs to the field of cell disruption technology, specifically relating to a cell disruption device. Background Technology

[0002] Cell disruption technology refers to the technique of using external force to break down cell membranes and cell walls, releasing the cell contents, including the target product. When separating and purifying a protein through cell disruption, the protein must first be released from the tissue or cell while maintaining its original natural state and activity. Therefore, appropriate methods must be used to disrupt the tissue and cells.

[0003] Currently, existing cell disruption devices typically only include agitation, resulting in minimal disturbance to the cell solution and inability to break down cells that have settled at the bottom of the cylinder, leading to substandard cell disruption rates. Therefore, there is an urgent need for a disruption device with better agitation capabilities to overcome the shortcomings of the existing technology. Utility Model Content

[0004] In view of the shortcomings of the existing technology, the purpose of this utility model is to provide a cell disruption device to solve the technical problem that the current disruption device has low disturbance to the solution, resulting in poor cell disruption rate.

[0005] To achieve the above objectives, the technical solution of this utility model is as follows: a cell disruption device, which includes a disruption bucket, a bucket lid disposed on the disruption bucket, and a disruption mechanism. The disruption device also includes a longitudinal disturbance mechanism for longitudinally agitating the liquid in the disruption bucket. The longitudinal disturbance mechanism includes a disturbance plate disposed between the disruption mechanism and the bottom of the disruption bucket, and a disturbance driving device for driving the disturbance plate to move longitudinally back and forth. The disturbance plate is provided with longitudinally penetrating flow holes.

[0006] Preferably, the disturbance driving device includes a disturbance motor disposed below the crushing barrel and a rotating shaft connected to the disturbance motor, the rotating shaft passing through the disturbance plate and being threadedly engaged.

[0007] Preferably, the rotating shaft and the bottom of the crushing barrel are sealed together by a lower bearing assembly.

[0008] Preferably, the disturbance driving device further includes a limiting post vertically connected to the bottom of the crushing barrel, the limiting post passing through the disturbance plate and being slidably connected.

[0009] Preferably, a stop plate is provided at the end of the limiting post that passes through the disturbance plate.

[0010] Preferably, the crushing mechanism includes two stirring shafts passing through the bucket lid, crushing blades disposed on the stirring shafts, and a crushing drive device disposed above the bucket lid, wherein the crushing blades on the two stirring shafts are arranged alternately.

[0011] Preferably, the two crushing blades are arranged as a pair, symmetrically on the stirring shaft, and multiple pairs of crushing blades are arranged at equal intervals on one stirring shaft.

[0012] Preferably, the crushing drive device includes a crushing motor, a drive gear connected to the crushing motor, and a driven gear coaxially arranged with the stirring shaft. The drive gear is disposed between the two driven gears, and the drive gear meshes with the driven gear.

[0013] The beneficial effects of adopting the technical solution of this utility model are as follows:

[0014] This invention incorporates a longitudinal agitation mechanism within the crushing chamber to agitate the solution vertically, promoting thorough mixing and preventing cells settled at the bottom from failing to contact the crushing mechanism, thus improving the cell breakage rate. The longitudinal agitation mechanism includes a disturbance plate and a disturbance drive device. The drive device moves the disturbance plate vertically, agitating the solution and promoting uniform mixing, increasing the probability of each cell contacting the crushing mechanism and improving the cell breakage rate. The disturbance plate is also provided with longitudinally penetrating flow holes to reduce movement resistance and conserve driving power. Attached Figure Description

[0015] Figure 1 A schematic cross-sectional view of an embodiment of a cell disruption device;

[0016] Figure 2 This is a schematic diagram of a disturbance plate in an embodiment of a cell disruption device.

[0017] in, Figure 1 , 2 In the middle, 1-crushing barrel, 2-barrel lid, 3-base, 4-upper bracket, 5-crushing motor, 6-drive gear, 7-driven gear, 8-upper bearing assembly, 9-stirring shaft, 10-crushing blade, 11-disturbance motor, 12-lower bracket, 13-lower bearing assembly, 14-rotating shaft, 15-disturbance plate, 16-limiting post, 17-discharge pipe, 18-threaded hole, 19-through hole, 20-flow hole. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, not all embodiments, and do not limit the scope of the present utility model.

[0019] In the description of this utility model, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0020] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0021] The specific implementation method is as follows:

[0022] Example 1, as Figure 1 , 2 As shown, a cell disruption device includes a disruption tank 1, a lid 2, a base 3, a disruption mechanism, and a longitudinal disturbance mechanism. The bottom of the disruption tank 1 is supported by the base 3, and the top is open and equipped with the lid 2. A discharge pipe 17 is also provided at the bottom of the side wall of the disruption tank 1 for discharging the disrupted solution. The disruption mechanism is mounted on the lid 2 and is used to disrupt cells. A longitudinal disturbance mechanism is provided between the end of the disruption mechanism that extends into the disruption tank 1 and the bottom of the disruption tank 1.

[0023] The longitudinal agitation mechanism includes an agitation plate 15 and an agitation driving device. The shape of the agitation plate 15 is the same as the cross-section of the inner cavity of the crushing barrel 1, but slightly smaller than the cross-section of the inner cavity of the crushing barrel 1. Multiple flow holes 20 are distributed on the agitation plate 15, and the flow holes 20 penetrate the agitation plate 15 longitudinally. Therefore, when the agitation plate 15 moves up and down, some solution flows through the flow holes 20, thus agitating the solution longitudinally while reducing the moving resistance of the agitation plate 15. The agitation driving device is located at the bottom of the crushing barrel 1 and is used to drive the agitation plate 15 to move reciprocally up and down along the axis of the crushing barrel 1, thereby achieving longitudinal agitation of the solution by the agitation plate 15 and promoting uniform mixing of the solution.

[0024] In this embodiment, a cell disruption device is used by placing a cell solution in a disruption tank 1, connecting a tank lid 2, and then disrupting the cells using a disruption mechanism. A disturbance drive is activated, causing a disturbance plate 15 to move up and down reciprocally, agitating the solution and promoting uniform mixing. This increases the probability of cells contacting the disruption mechanism, thereby improving the cell disruption rate. The flow holes 20 on the disturbance plate 15 reduce the moving resistance of the plate, saving power output from the disturbance drive and thus reducing energy consumption and emissions.

[0025] Furthermore, the disturbance drive device includes a disturbance motor 11 and a rotating shaft 14. The disturbance motor 11 is located below the crushing barrel 1 and is supported by a lower bracket 12 mounted on the base 3. The rotating shaft 14 is coaxially connected to the shaft of the disturbance motor 11. The rotating shaft 14 passes through the crushing barrel 1 from the bottom upwards and passes through a threaded hole 18 on the disturbance plate 15, where it is threadedly engaged. Based on this, the disturbance motor 11 starts and drives the rotating shaft 14 to rotate. The threaded engagement between the rotating shaft 14 and the disturbance plate 15 causes the disturbance plate 15 to move longitudinally. The disturbance motor 11 rotates in both directions to achieve the reciprocating up-and-down movement of the disturbance plate 15, thus realizing longitudinal disturbance.

[0026] Furthermore, a lower bearing assembly 13 is provided at the connection between the rotating shaft 14 and the crushing barrel 1 to achieve a sealed connection while ensuring the free rotation of the rotating shaft 14.

[0027] Furthermore, the disturbance drive device also includes a limiting post 16. The limiting post 16 is vertically connected to the bottom of the crushing barrel 1. The limiting post 16 passes through the through hole 19 provided on the disturbance plate 15 to realize the sliding connection between the disturbance plate 15 and the limiting post 16, which is used to limit the disturbance plate 15 from rotating with the rotating shaft 14 and ensure that the rotating shaft 14 and the disturbance plate 15 achieve threaded drive.

[0028] In this embodiment, two limiting posts 16 are provided, symmetrically arranged on both sides of the rotating shaft 14.

[0029] Furthermore, a stop plate is vertically provided at the upper end of the limiting post 16 that passes through the disturbance plate 15 to prevent the disturbance plate 15 from slipping off the limiting post 16 and improve safety during use.

[0030] Furthermore, the crushing mechanism includes stirring shafts 9, crushing blades 10, and a crushing drive device. Two stirring shafts 9 are provided, symmetrically arranged relative to the central axis of the lid 2, and sealed to the lid 2 via an upper bearing assembly 8. The two stirring shafts 9 are synchronously driven by the crushing drive device located above the lid 2. The crushing blades 10 are vertically connected to the stirring shafts 9, and the crushing blades 10 on the two stirring shafts 9 are staggered, so they do not affect each other's rotation. Based on this, when the crushing drive device is activated, it drives the two stirring shafts 9 to rotate, which in turn drives the crushing blades 10 to rotate, thereby achieving cell crushing.

[0031] Furthermore, the two crushing blades 10 form a pair, and the pair of crushing blades 10 are symmetrically connected to the stirring shaft 9. Multiple pairs of crushing blades 10 are spaced apart on one stirring shaft 9. The crushing blades 10 on the two stirring shafts 9 are arranged in a gear-like meshing pattern, with appropriate gaps between the intersecting crushing blades 10. Based on this, the density of the crushing blades 10 is increased, and the crushing blades 10 on both sides will intersect when the two stirring shafts 9 rotate, thereby improving the crushing effect.

[0032] Furthermore, the crushing drive device includes a crushing motor 5, a drive gear 6, and a driven gear 7. Two stirring shafts 9 extend upwards through the upper end of the lid 2, and the driven gear 7 is coaxially mounted. The drive gear 6 is positioned between the two driven gears 7 and meshes with them. The drive gear 6 is coaxially connected to the shaft of the crushing motor 5, which is supported by an upper bracket 4 connected to the lid 2. Based on this, when the crushing motor 5 starts, it drives the drive gear 6 to rotate, thereby causing the two driven gears 7 to rotate synchronously and in opposite directions. This causes the crushing blades 10 on the two stirring shafts 9 to rotate in opposite directions, making it easier to crush cells during the blades' movement and improving the crushing effect.

[0033] Example 2: A cell disruption device, which differs from the above examples in that the disturbance drive device uses a hydraulic cylinder to drive the disturbance plate to move up and down reciprocally. Compared with the motor-driven structure, it is simpler. Other structures will not be described in detail here.

[0034] The present invention has been described above by way of example with reference to the accompanying drawings. Obviously, the specific implementation of the present invention is not limited to the above-described manner. Any non-substantial improvements made using the technical solution of the present invention, or the direct application of the concept and technical solution of the present invention to other occasions without modification, are all within the protection scope of the present invention.

Claims

1. A cell disruption device, the disruption device comprising a disruption bucket, a bucket cover provided on the disruption bucket, and a disruption mechanism, characterized by, The crushing device further comprises a longitudinal disturbance mechanism for longitudinally disturbing the liquid in the crushing barrel, the longitudinal disturbance mechanism comprising a disturbance plate arranged between the crushing mechanism and the bottom of the crushing barrel and a disturbance driving device for driving the disturbance plate to longitudinally reciprocate, the disturbance plate being provided with longitudinal through-flow holes.

2. A cell disruption device according to claim 1, wherein The disturbance driving device comprises a disturbance motor arranged below the crushing barrel and a rotating shaft connected with the disturbance motor, the rotating shaft penetrating the disturbance plate and being threadedly connected.

3. A cell disruption device according to claim 2, wherein, The rotating shaft and the bottom of the crushing barrel are sealingly connected through a lower bearing assembly.

4. A cell disruption device according to claim 3, wherein The disturbance driving device further comprises a limiting column perpendicularly connected with the bottom of the crushing barrel, the limiting column penetrating the disturbance plate and being slidingly connected.

5. A cell disruption device according to claim 4, wherein, The end of the limiting column penetrating the disturbance plate is provided with a stop plate.

6. A cell disruption device according to claim 1, wherein The crushing mechanism comprises two stirring shafts penetrating the barrel cover, crushing knives arranged on the stirring shafts and a crushing driving device arranged above the barrel cover, the crushing knives on the two stirring shafts being staggered.

7. A cell disruption device according to claim 6, wherein The two crushing knives are a pair and symmetrically arranged on the stirring shafts, and a plurality of pairs of the crushing knives are equidistantly arranged on one stirring shaft.

8. A cell disruption device according to claim 7, wherein, The crushing driving device comprises a crushing motor, a driving gear connected with the crushing motor and a driven gear coaxially arranged with the stirring shafts, the driving gear being arranged between the two driven gears and being engaged with the driven gears.

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