Grinding bag for plant tissue extraction

Abstract

The utility model belongs to the technical field of plant tissue extraction devices, and particularly discloses a grinding bag for plant tissue extraction, which comprises a bag body, the bag body is provided with a bag opening, the bag opening is connected with a sealing structure, the bag opening is opened or closed through the sealing structure, and a grinding structure is arranged on the inner wall of the bag body. The bag body is provided with a tissue component extraction structure away from the bag opening. The grinding structure, the sealing structure and the like are innovatively designed, the problems that an existing grinding device is not ideal in grinding effect, prone to cross contamination, inconvenient to operate and the like are effectively solved, performance tests and optimization show that the grinding device is excellent in grinding efficiency, cross contamination prevention, operation convenience and the like, and the grinding effect is good. The method can meet the requirement for juice grinding in test strip detection of the Citrus Huanglongbing, provides powerful technical support for rapid and accurate detection of the Citrus Huanglongbing, and has a wide application prospect.

Description

Technical Field

[0001] This utility model belongs to the technical field of plant tissue extraction devices, specifically relating to a grinding bag for plant tissue extraction. Background Technology

[0002] Citrus Huanglongbing (HLB) is a devastating disease that severely damages the citrus industry. It is caused by a Gram-negative bacterium, *Candidatus Liberibacterasiaticus*, which parasitizes only within the phloem. This pathogen is difficult to culture artificially, and its transmission route is unique. In the early stages of infection, some new shoots exhibit uniform yellowing, known as "yellow shoots." This yellowing is distinctly different from yellowing caused by nutrient deficiencies; HLB-infected leaves show more uniform yellowing, and the veins also turn yellow. As the disease progresses, the leaves develop mottled yellowing, with irregular yellow-green patches. Spring shoots are generally less affected, while summer and autumn shoots are more severely affected. Currently, nucleic acid or antibody-based test strips are widely used due to their speed and simplicity. However, before conducting test strip tests, juice extraction from citrus leaves and other samples is necessary, and efficient, high-quality juice grinding equipment plays a crucial role in obtaining suitable test juice.

[0003] Existing grinding devices have several problems in the application of citrus Huanglongbing (HLB) detection, such as insufficient grinding, easy sample contamination, and inconvenient operation. For example, devices with fixed grinding heads can cause cross-contamination between samples if cleaning is not thorough. Since HLB is a contagious disease, cross-contamination can lead to misinterpretations of test results, posing a serious threat to disease control. Secondly, the control interfaces of some electric grinding devices are complex, making them difficult for grassroots testing personnel to master. Therefore, developing a juice grinding device specifically designed for HLB test strip detection is of great significance.

[0004] To address the problems of sample contamination and operational inconvenience caused by existing grinding devices, it is necessary to improve the structure of the grinding device to solve the current technical problems. Utility Model Content

[0005] The purpose of this invention is to provide a grinding bag for plant tissue extraction. The grinding process does not involve electromechanical operation, making it simple, convenient, and efficient to use. Moreover, each bag stores one sample, eliminating cross-contamination and resulting in more accurate sample test results.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a grinding bag for extracting plant tissue, comprising a bag body, the bag body having a bag opening, the bag opening being connected to a sealing structure, the bag opening being opened or closed through the sealing structure, the inner wall of the bag body being provided with a grinding structure, and the bag body being provided with a tissue component extraction structure away from the bag opening.

[0007] To better realize this utility model, the bag body is formed by bonding any three sides of two rectangular rigid PVC plastic sheets arranged opposite each other, and the two unbonded sides form the bag opening.

[0008] To better realize this utility model, the bag body is a transparent structure.

[0009] To better realize this utility model, the sealing structure includes a limiting protrusion and a limiting ring arranged in parallel. The limiting protrusion is shaped to the inner circle of the limiting ring. The limiting protrusion and the limiting ring are respectively fixedly connected to the bag body on both sides of the bag opening. The limiting ring has a strip-shaped notch facing the limiting protrusion. The width of the strip-shaped notch is smaller than the maximum diameter of the cross section of the limiting protrusion.

[0010] To better realize this utility model, the sealing structure further includes a sealing slider, the sealing slider having a limiting groove, the bag body having a limiting strip away from the limiting protrusion, the bag bodies on both sides of the bag opening being movably limited and connected to the limiting groove by the limiting strip and the limiting ring respectively, and a separator strip inserted into the bag opening is fixed at one end of the sealing slider.

[0011] To better realize this utility model, the outer surface of the sealing slider has anti-slip texture.

[0012] To better realize this utility model, the grinding structure includes multiple hemispherical abrasive protrusions and multiple conical abrasive protrusions. The hemispherical abrasive protrusions and conical abrasive protrusions are integrally formed on the opposing inner walls of the bag body, and the conical abrasive protrusions correspond to the interval between adjacent hemispherical abrasive protrusions.

[0013] To better realize this utility model, the tissue component extraction structure includes a liquid outlet tube, which is connected to the inner cavity of the bag body. The liquid outlet tube is equipped with a valve near the liquid outlet and a filter screen near the liquid inlet.

[0014] Beneficial effects:

[0015] This invention relates to a grinding bag. Citrus leaves are simply placed inside the bag's inner cavity. The grinding structure breaks down the plant tissue walls, allowing plant tissue components to flow out and remain inside the bag. These components are then extracted for testing. The device features innovative designs in its grinding and sealing structures, effectively solving problems such as unsatisfactory grinding effects, cross-contamination, and inconvenient operation found in existing grinding devices. After performance testing and optimization, this grinding device demonstrates excellent performance in grinding efficiency, prevention of cross-contamination, and ease of operation. It meets the requirements for juice grinding in citrus Huanglongbing test strip detection, providing strong technical support for the rapid and accurate detection of citrus Huanglongbing and showing broad application prospects. Attached Figure Description

[0016] Figure 1 This is a front view of the grinding bag of this utility model;

[0017] Figure 2 This is an enlarged view of point M in this utility model;

[0018] Figure 3 This is a cross-sectional view of the grinding bag AA of this utility model;

[0019] Figure 4 This is a partial view of the cross-section of the grinding bag BB of this utility model.

[0020] In the diagram: 1. Bag body; 11. Limiting strip; 2. Bag opening; 3. Sealing structure; 31. Limiting protrusion; 32. Limiting retaining ring; 33. Strip-shaped notch; 34. Sealing slider; 341. Limiting groove; 342. Separator strip; 343. Anti-slip texture; 4. Grinding structure; 41. Hemispherical abrasive protrusion; 42. Conical abrasive protrusion; 5. Tissue component extraction structure; 51. Liquid outlet pipe; 52. Valve; 53. Filter screen. Detailed Implementation

[0021] Example

[0022] like Figure 1 - Figure 4 As shown, a grinding bag for plant tissue extraction includes a bag body 1, the bag body 1 having a bag opening 2, the bag opening 2 being connected to a sealing structure 3, the bag opening 2 being opened or closed through the sealing structure 3, the inner wall of the bag body 1 being provided with a grinding structure 4, and the bag body 1 being provided with a tissue component extraction structure 5 away from the bag opening 2.

[0023] The working principle of this utility model can be summarized as follows:

[0024] When it is necessary to extract juice from citrus leaf samples, first open the bag opening 2 through the sealing structure 3, place the citrus leaf sample into the inner cavity of the bag body 1, and then close the bag opening 2 to prevent juice from splashing out during the grinding process. Then press the bag body 1, and the grinding structure 4 will grind and break the cell walls of the plant tissue. The plant tissue components flow out from the cell walls and are stored in the inner cavity of the bag body 1. Then, the plant tissue components are discharged into the test strip for detection through the tissue component extraction structure 5.

[0025] Preferably, the bag body 1 is formed by bonding any three sides of two rectangular rigid PVC plastic sheets that are arranged opposite each other, and the two unbonded sides form the bag opening 2. The volume of the bag body 1 is designed to hold 5-10 grams of citrus leaf samples. It is of moderate size, the PVC plastic sheets are lightweight and inexpensive, and easy to carry. At the same time, one grinding bag grinds one sample, which will not cause cross-contamination between samples during grinding.

[0026] Preferably, the bag body 1 is transparent to facilitate observation of the degree of cell wall disruption during plant tissue grinding.

[0027] Preferably, the sealing structure 3 includes a parallel limiting protrusion 31 and a limiting ring 32. The limiting protrusion 31 conforms to the inner circle of the limiting ring 32. The limiting protrusion 31 and the limiting ring 32 are respectively fixedly connected to the bag body 1 on both sides of the bag opening 2. The limiting ring 32 has a strip-shaped notch 33 facing the limiting protrusion 31, and the width of the strip-shaped notch 33 is smaller than the maximum diameter of the cross-section of the limiting protrusion 31. This sealing structure 3 is easy to operate. The user only needs to first insert the limiting protrusion 31 at one end into the limiting ring 32, and then use two fingers to squeeze and move towards the other end to complete the sealing of the bag opening 2. To open, simply pull the bag body 1 on both sides of the bag opening 2 with both palms, which is very convenient.

[0028] Preferably, the sealing structure 3 further includes a sealing slider 34, which has a limiting groove 341. The bag body 1 has a limiting strip 11 away from the limiting protrusion 31. The bag bodies 1 on both sides of the bag opening 2 are movably limited and connected to the limiting groove 341 by the limiting strip 11 and the limiting ring 32, respectively. A separator strip 342 is fixedly provided at one end of the sealing slider 34 and inserted into the bag opening 2. The sealing is done by replacing the human finger with the sealing slider 34. The finger does not move relative to the sealing slider 34, which can avoid friction damage to the human finger. When the sealing slider 34 moves towards the separator strip 342, the end of the sealing slider 34 away from the separator strip 342 will continuously squeeze the limiting protrusion 31 into the limiting ring 32, and the bag opening 2 will be closed. Conversely, the separator strip 342 will continuously remove the limiting protrusion 31 from the limiting ring 32, and the bag opening 2 will be opened.

[0029] Preferably, the outer surface of the sealing slider 34 has anti-slip texture 343, so that when the user pinches the sealing slider 34, it is not easy to slip off under the action of the anti-slip texture 343, thus improving the safety of use.

[0030] Preferably, the grinding structure 4 includes multiple hemispherical abrasive protrusions 41 and multiple conical abrasive protrusions 42. The hemispherical abrasive protrusions 41 and the conical abrasive protrusions 42 are integrally formed on opposite inner walls of the bag body 1, and the conical abrasive protrusions 42 correspond to the intervals between adjacent hemispherical abrasive protrusions 41. The hemispherical abrasive protrusions increase the friction during sample grinding, promoting cell disruption. Simultaneously, the conical abrasive protrusions 42, with their tips constantly intersecting the hemispherical abrasive protrusions, further enhance the cell wall disruption effect of the abrasive protrusions on plant tissues during grinding, allowing more plant tissue components to flow out from the cell walls. In use, it only requires rubbing and pressing the bag body 1 with a finger or a relatively hard tool such as a ballpoint pen. The dynamic friction force can reach 5-8N. The structure is simple and requires no electromechanical structure to achieve plant tissue grinding. No additional energy supply is needed, making it suitable for various field sampling scenarios. It is convenient to operate and provides good grinding results.

[0031] Preferably, the tissue component extraction structure 5 includes an outlet pipe 51, which is connected to the inner cavity of the bag body 1. The outlet pipe 51 is equipped with a valve 52 near the outlet and a filter screen 53 near the inlet. The filter screen 53 is a 50-mesh plastic screen, which can effectively intercept the ground plant tissue. A T-shaped plastic valve 52 is also provided for easy insertion and removal, ensuring good sealing and convenient operation. The outlet pipe 51 is located at the end of the bag body 1 furthest from the bag opening 2. By tilting the bag body 1, all the crude plant extract in the bag can be poured out without spilling the plant tissue due to improper operation. After use, the T-shaped valve 52 can be reinserted to retain the plant tissue for subsequent repeated operations.

[0032] This application uses leaves and branches of different citrus varieties and at different growth stages as test samples, and extracts juice using both the proposed grinding device and a traditional grinding device. The amount of juice extracted and the content of active ingredients (such as nucleic acid or protein concentration) in the juice are compared between the two devices within the same time period. Test results show that the juice extraction volume of the proposed grinding device is 30%–50% higher than that of the traditional device, and the content of active ingredients is also significantly increased, indicating that the proposed device has higher grinding efficiency.

[0033] Finally, it should be noted that the above are merely preferred embodiments of this utility model and are not intended to limit the scope of protection of this utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the scope of protection of this utility model.

Claims

1. A grinding bag for plant tissue extraction, characterized by, The utility model provides a bag, including the bag body (1), the bag body (1) has a bag mouth (2), the bag mouth (2) is connected with the closure structure (3), the bag mouth (2) is opened or closed through the closure structure (3), the closure structure (3) includes parallelly arranged limiting lug (31) and limiting snap ring (32), the limiting lug (31) is along with the limiting snap ring (32) inner ring, the limiting lug (31) and limiting snap ring (32) are fixedly connected on the bag body (1) of bag mouth (2) two sides respectively, the limiting snap ring (32) has the strip-shaped gap (33) towards the limiting lug (31), the width of strip-shaped gap (33) is less than the maximum diameter of cross section of limiting lug (31); The inner wall of the bag body (1) is provided with a grinding structure (4), the grinding structure (4) includes a plurality of semispherical abrasive protrusions (41) and a plurality of conical abrasive protrusions (42), the semispherical abrasive protrusions (41) and the conical abrasive protrusions (42) are integrally formed on the opposite inner walls of the bag body (1), and the conical abrasive protrusions (42) correspond to the intervals between adjacent semispherical abrasive protrusions (41). The bag body (1) is provided with a tissue component extraction structure (5) away from the bag mouth (2).

2. The mill bag of claim 1, wherein, The bag body (1) is formed by bonding any three edges of two oppositely arranged rectangular hard PVC plastic sheets, and the two unbonded edges form the bag mouth (2).

3. The mill bag of claim 1, wherein, The bag body (1) is a transparent structure.

4. The mill bag of claim 1, wherein, The closure structure (3) further includes a closure sliding block (34) having a limiting groove (341), the bag body (1) has a limiting strip (11) away from the limiting lug (31), and the bag body (1) on both sides of the bag mouth (2) is movably limitedly connected to the limiting groove (341) through the limiting strip (11) and the limiting snap ring (32), and one end of the closure sliding block (34) is fixedly provided with a separation strip (342) inserted into the bag mouth (2).

5. The mill bag of claim 4, wherein, The outer side of the closure sliding block (34) has an anti-skid pattern (343).

6. The mill bag of claim 1, wherein, The tissue component extraction structure (5) includes a liquid outlet pipe (51) connected to the inner cavity of the bag body (1), and the liquid outlet pipe (51) is provided with a valve (52) near the liquid outlet and a filter screen (53) near the liquid inlet.

Images