Cell resuscitation adapter
By designing the dispensing, ejection, sealing, and adjustment mechanisms of the cell resuscitation adapter, the problem of uneven distribution of resuscitation fluid was solved, cell survival rate was improved and the risk of damage was reduced, achieving efficient and safe operation of the cell resuscitation process.
Patent Information
- Application Number
- CN202520032437.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-07
- Publication Date
- 2026-01-06
- Estimated Expiration
- 2035-01-07
AI Technical Summary
In existing technologies, the resuscitation fluid cannot be evenly distributed in the cell culture tube when manually aspirated, resulting in a reduced survival rate of individual cultured cells.
A cell resuscitation adapter was designed, comprising a dispensing mechanism, an ejection mechanism, a sealing mechanism, and an adjustment mechanism. It achieves uniform distribution of resuscitation fluid through a vacuum pump and microfluidic channels, uses highly biocompatible polymer materials and a sealing mechanism to prevent contamination, and monitors and controls humidity and temperature in real time.
It improved cell survival rate, ensured uniform cell distribution and sealing during resuscitation, reduced the risk of cell damage, and optimized resuscitation conditions.
Smart Images
Figure CN223766318U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of cell culture technology, and in particular to a cell resuscitation adapter. Background Technology
[0002] Cell culture is a biotechnology that involves culturing and propagating cells in an artificially controlled environment. This technique is usually carried out in a laboratory, using specific culture media, temperature, humidity, and gas conditions to simulate the natural growth environment of cells in a living organism. Cell culture is used in a variety of research and applications, including basic biological research, drug development, vaccine production, and genetic engineering. In the process of cell culture, we usually need to use a cell resuscitation adapter to complete this crucial step.
[0003] A cell resuscitation adapter is a specially designed tool that helps to quickly and effectively restore frozen cells to a viable state suitable for culture after they have been removed from liquid nitrogen. By using a cell resuscitation adapter, it is possible to ensure that cells are not subjected to excessive temperature shocks during the resuscitation process, thereby minimizing cell damage and mortality, and improving cell survival rate and culture success rate.
[0004] The existing method for resuscitating cells involves manually drawing resuscitating fluid using a dropper and dripping it into the cell culture tube. However, the manually drawn resuscitating fluid cannot be evenly distributed in the cell culture tube, and the survival rate of individual cultured cells is reduced. To address these issues, a cell resuscitating adapter is proposed. Utility Model Content
[0005] To overcome the above shortcomings, this invention provides a cell resuscitation adapter, which aims to improve the problem that artificially aspirated resuscitation fluid cannot be evenly distributed in the cell culture tube, and that the survival rate of individual cultured cells is reduced in the prior art.
[0006] To achieve the above objectives, this utility model adopts the following technical solution: a cell resuscitation adapter, comprising a housing, wherein a liquid dispensing mechanism is provided inside the housing for uniformly distributing the resuscitation liquid and improving cell survival rate; an ejection mechanism is provided at the bottom inner side of the housing for ejecting culture tubes for easy handling; a sealing mechanism is provided at the top of the housing for sealing the entire culture device and effectively preventing external contamination; an adjustment mechanism is provided at the front of the housing for controlling the humidity inside the housing; the liquid dispensing mechanism includes an inlet pipe, one end of which is connected to... On the left side of the box, multiple incubation chambers are provided inside the box. A shelf is fixedly connected to the middle of the inner wall of each of the multiple incubation chambers. A liquid storage box is fixedly connected to the bottom of the inner side of each of the multiple incubation chambers. The liquid inlet pipe is connected to the multiple liquid storage boxes. The right end of the liquid inlet pipe penetrates the inner wall of the box and is connected to the liquid outlet pipe. The top of each of the multiple liquid storage boxes is connected to the connecting pipe. The top of each of the multiple connecting pipes penetrates the bottom of the shelf and is connected to the vacuum pipe. The top of each of the multiple vacuum pipes is connected to the vacuum pump. One end of the vacuum pipe penetrates the inner wall of the box and is connected to the vacuum pump. A solenoid valve is installed at the top of the outer wall of each of the multiple connecting pipes. A microfluidic channel is connected to the top of the outer wall of each of the multiple connecting pipes.
[0007] As a further description of the above technical solution:
[0008] The sealing mechanism includes a top cover, the bottom of which is fixedly connected to the top of the box. Multiple access ports are provided on the top of the top cover. A rotating shaft is rotatably connected to the top of each top cover. Sealing plates are rotatably connected to the outer walls of each of the rotating shafts. Rubber rings are fixedly connected to the outer walls of each of the sealing plates. A slot is provided on the front side of each of the sealing plates. A sliding groove is provided on the front side of the top of the top cover. A slider is slidably connected inside each of the sliding grooves. A spring is fixedly connected to the front side of each of the sliders. A locking plate is fixedly connected to the rear side of each of the sliders.
[0009] As a further description of the above technical solution:
[0010] The sealing mechanism also includes multiple buckle plates, the bottoms of which are fixedly connected to the top of the slider.
[0011] As a further description of the above technical solution:
[0012] The ejection mechanism includes multiple electric push rods, the bottom of which is fixedly connected to the bottom of the inner side of the incubation chamber. One end of each of the multiple electric push rods is fixedly connected to a top plate. The bottom of each of the multiple layers is connected to a placement cylinder. The outer wall of the top plate is slidably connected to the inner wall of the placement cylinder.
[0013] As a further description of the above technical solution:
[0014] The adjustment mechanism includes multiple humidity sensors, all of which are fixedly connected to the inner wall of the incubation chamber. A liquid storage cylinder is provided on the front side of the chamber, and an atomizer is connected to the top of the liquid storage cylinder. An atomizing tube is connected to the top of the atomizer, and the rear end of the atomizing tube passes through the front side of the chamber and is connected to an atomizing nozzle.
[0015] As a further description of the above technical solution:
[0016] The outer wall of each liquid storage cylinder is fixedly connected with a reinforcing plate, and the rear sides of the two reinforcing plates are fixedly connected to the front side of the box body.
[0017] As a further description of the above technical solution:
[0018] A controller is fixedly connected to the front side of the box, and temperature sensors are fixedly connected to the inner walls of the multiple incubation chambers.
[0019] As a further description of the above technical solution:
[0020] The controller is electrically connected to the solenoid valve, vacuum pump, atomizer, humidity sensor and temperature sensor respectively, and a display screen is fixedly connected to the top of the controller.
[0021] This utility model has the following beneficial effects:
[0022] In this invention, modular culture processing is achieved through multiple culture chambers, improving cell survival rate. The inner wall material of the culture chamber is made of a highly biocompatible polymer, which can reduce the risk of cell damage. Resuscitation fluid is injected through an inlet pipe, which is connected to a reservoir at the bottom of each chamber. After the resuscitation fluid enters the reservoir, the pipe is closed, and a vacuum pump is activated to apply negative pressure to the suction pipe. At this time, the resuscitation fluid is extracted through a connecting pipe, and then the connecting pipe is closed by controlling a solenoid valve, allowing the resuscitation fluid to flow into the cell test tube through a microfluidic channel, achieving uniform distribution of the resuscitation fluid and thus improving cell survival rate.
[0023] In this invention, a rubber ring is used to seal the sealing plate and the retrieval port, improving sealing efficiency and effectively preventing external contamination. To ensure the sealing plate's stability, a locking plate is inserted into the slot. When the sealing plate needs to be opened, the latch plate can be pulled backward, causing the slider to push the spring backward, thus disengaging the locking plate from the slot and opening the sealing plate. A humidity sensor monitors changes in the cell environment in real time, facilitating the optimization of resuscitation conditions. The nebulizer is also controlled to precisely spray moisture into the culture chamber through the atomizing nozzle, achieving humidification. Attached Figure Description
[0024] Figure 1 This is a perspective view of a cell resuscitation adapter proposed in this utility model;
[0025] Figure 2 This is a schematic diagram of the structure of a cell resuscitation adapter proposed in this utility model;
[0026] Figure 3 This is an exploded view of the ejection mechanism of a cell resuscitation adapter proposed in this utility model;
[0027] Figure 4 This is an exploded view of the sealing mechanism of a cell resuscitation adapter proposed in this utility model;
[0028] Figure 5 This is a schematic diagram of the card block of a cell resuscitation adapter proposed in this utility model.
[0029] Legend:
[0030] 1. Chamber; 2. Mounting plate; 3. Incubation chamber; 4. Shelf; 5. Placement cylinder; 6. Inlet pipe; 7. Storage box; 8. Outlet pipe; 9. Gas pipe; 10. Solenoid valve; 11. Microfluidic channel; 12. Vacuum pump; 13. Connecting pipe; 14. Top cover; 15. Retrieval port; 16. Rotating shaft; 17. Sealing plate; 18. Slot; 19. Rubber ring; 20. Slide groove; 21. Slider; 22. Locking plate; 23. Spring; 24. Storage cylinder; 25. Atomizer; 26. Atomizing tube; 27. Atomizing nozzle; 28. Reinforcing plate; 29. Controller; 30. Display screen; 31. Top plate; 32. Temperature sensor; 33. Humidity sensor; 34. Electric push rod. Detailed Implementation
[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0032] Reference Figure 1 , Figure 2 and Figure 3This utility model provides an embodiment of a cell resuscitation adapter, comprising a housing 1, an internal liquid distribution mechanism for uniformly distributing resuscitation liquid to improve cell survival rate, an ejection mechanism for ejecting culture tubes for easy retrieval, a sealing mechanism for sealing the entire culture device to effectively prevent external contamination, and an adjustment mechanism for controlling the humidity inside the housing 1. The ejection mechanism includes multiple electric push rods 34, the bottoms of which are fixedly connected to the bottom of the culture chamber 3, and one end of each push rod 34 is fixedly connected to a top plate 31. The bottoms of multiple shelves 4 are connected to a placement cylinder 5, and the outer wall of the top plate 31 is slidably connected to the inner wall of the placement cylinder 5. Cell tubes are placed in the placement cylinder 5, and when they are to be retrieved, the electric push rods 34 can be activated to drive the top plate 31 to eject the cell tubes for easy manual retrieval.
[0033] The dispensing mechanism includes an inlet pipe 6, one end of which is connected to the left side of the housing 1. Multiple culture chambers 3 are located inside the housing 1, enabling modular culture processing and improving cell survival rate. The inner wall material of the culture chambers 3 is made of a highly biocompatible polymer to reduce the risk of cell damage. A shelf 4 is fixedly connected to the middle of the inner wall of each culture chamber 3, and a storage box 7 is fixedly connected to the bottom of the inner side of each culture chamber 3. The inlet pipe 6 is connected to the multiple storage boxes 7, and resuscitation fluid is injected through the inlet pipe 6. The inlet pipe 6 is connected to the storage box 7 at the bottom of each chamber. After the resuscitation fluid enters the storage box 7, the pipe is closed. The right end of the inlet pipe 6 penetrates the inner wall of the housing 1 and is connected to an outlet pipe 8. Multiple storage boxes... Each box 7 has a connecting tube 13 at its top. The top of each connecting tube 13 penetrates the bottom of the shelf 4 and is connected to a vacuum tube 9. The top of each vacuum tube 9 is connected to a vacuum pump 12. By activating the vacuum pump 12, negative pressure is applied to the vacuum tube 9, at which point the resuscitation fluid is extracted through the connecting tube 13. Then, the connecting tube 13 is closed by controlling the solenoid valve 10, and the vacuum pump 12 is also closed. The top of the outer wall of each connecting tube 13 is equipped with a solenoid valve 10, and the top of the outer wall of each connecting tube 13 is connected to a microfluidic channel 11, allowing the resuscitation fluid to flow into the cell tube through the microfluidic channel 11, achieving uniform distribution of the resuscitation fluid and thus improving the cell survival rate.
[0034] Reference Figure 1 , Figure 4 and Figure 5The sealing mechanism includes a top cover 14, the bottom of which is fixedly connected to the top of the housing 1. The top cover 14 seals the housing 1. Multiple access ports 15 are provided on the top of the top cover 14, allowing for the retrieval and placement of test tubes. A rotating shaft 16 is rotatably connected to the top of each top cover 14. Sealing plates 17 are rotatably connected to the outer walls of the multiple rotating shafts 16. Rubber rings 19 are fixedly connected to the outer walls of the multiple sealing plates 17. The sealing plates 17 and the top cover 14 are connected via the rotating shafts 16. The sealing plate 17 can be rotated to open and close. Each sealing plate 17 has a slot 18 on its front side. A rubber ring 19 seals the sealing plate 17 with the access port 15, improving sealing efficiency and effectively preventing external contamination. Each top front side of the top cover 14 has a sliding groove 20. A slider 21 is slidably connected inside each sliding groove 20. A spring 23 is fixedly connected to the front side of each slider 21, and a locking plate 22 is fixedly connected to the rear side of each slider 21. The sealing mechanism also includes multiple latching plates 2. After the sealing plate 17 is closed... To ensure the sealing plate 17 is secure, a locking plate 22 is inserted into a slot 18 to fix the sealing plate 17. When the sealing plate 17 needs to be opened, the latch plate 2 is pulled backward, causing the slider 21 to push the spring 23 backward, disengaging the locking plate 22 from the slot 18, thus opening the sealing plate 17. The bottoms of multiple latch plates 2 are fixedly connected to the top of the slider 21. The adjustment mechanism includes multiple humidity sensors 33, which monitor the humidity in the chamber in real time. Each humidity sensor 33 is fixedly connected to the inner wall of the incubation chamber 3. A liquid storage cylinder 24 is provided on the front side of the box 1. An atomizer 25 is connected to the top of the liquid storage cylinder 24. An atomizing tube 26 is connected to the top of the atomizer 25. The rear end of the atomizing tube 26 passes through the front side of the box 1 and is connected to an atomizing nozzle 27. The atomizer 25 is controlled to accurately spray moisture into the incubation chamber 3 through the atomizing nozzle 27 to achieve humidification. A reinforcing plate 28 is fixedly connected to the outer wall of the liquid storage cylinder 24. The rear sides of the two reinforcing plates 28 are fixedly connected to the front side of the box 1.
[0035] Reference Figure 1 , Figure 2 and Figure 3 A controller 29 is fixedly connected to the front of the housing 1. The controller 29 allows for simple operation and control of the equipment on the entire device. Temperature sensors 32 are fixedly connected to the inner walls of multiple culture chambers 3. The temperature sensors 32 can detect the temperature in each chamber in real time to ensure that the cells maintain the optimal temperature during the recovery process. The controller 29 is electrically connected to the solenoid valve 10, vacuum pump 12, nebulizer 25, humidity sensor 33 and temperature sensor 32 respectively. A display screen 30 is fixedly connected to the top of the controller 29.
[0036] Working principle: First, the cell tubes are placed in the placement tube 5. When removing them, the top plate 31 is pushed out by the electric push rod 34, making it easy to remove manually. Modular culture processing is achieved through multiple culture chambers 3, which improves cell survival rate. The inner wall material of the culture chamber 3 is a highly biocompatible polymer, which can reduce the risk of cell damage. The resuscitation liquid is injected through the liquid inlet pipe 6, which is connected to the liquid storage box 7 at the bottom of each chamber. After the resuscitation liquid enters the liquid storage box 7, the pipe is closed. The vacuum pump 12 is started to create negative pressure on the suction pipe 9. At this time, the resuscitation liquid is extracted through the connecting pipe 13. Then, the connecting pipe 13 is closed by controlling the solenoid valve 10, so that the resuscitation liquid flows into the cell tube through the microfluidic channel 11, achieving uniform distribution of the resuscitation liquid and thus improving the cell survival rate.
[0037] Furthermore, the top cover 14 seals the chamber 1, and the retrieval port 15 allows for the retrieval and placement of test tubes. The sealing plate 17 and the retrieval port 15 are sealed with a rubber ring 19 to improve sealing efficiency and effectively prevent external contamination. To ensure the firmness of the sealing plate 17, a locking plate 22 is inserted into the slot 18 to fix the sealing plate 17. When the sealing plate 17 needs to be opened, the buckle plate 2 is pulled backward, causing the slider 21 to drive the spring 23 to press backward, causing the locking plate 22 to disengage from the slot 18, thus opening the sealing plate 17. The humidity sensor 33 monitors changes in the cell environment in real time to optimize resuscitation conditions and controls the nebulizer 25 to precisely spray moisture into the culture chamber 3 through the atomizing nozzle 27 for humidification.
[0038] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.
Claims
1. A cell thawing adapter comprising a box (1), characterized in that: The inside of the box (1) is provided with a liquid distribution mechanism for uniformly distributing the resuscitation liquid and improving the survival rate of cells. The inside bottom of the box (1) is provided with an ejection mechanism for ejecting the incubation test tube and facilitating taking. The top of the box (1) is provided with a sealing mechanism for sealing the entire incubation device and effectively preventing external pollution. The front side of the box (1) is provided with an adjusting mechanism for controlling the humidity inside the box (1). The liquid distribution mechanism comprises a liquid inlet pipe (6), one end of the liquid inlet pipe (6) is communicated with the left side of the box (1), a plurality of incubation cavities (3) are formed in the inside of the box (1), the inner wall middle portions of the plurality of incubation cavities (3) are fixedly connected with a plurality of layer plates (4), the inside bottoms of the plurality of incubation cavities (3) are fixedly connected with a plurality of liquid storage boxes (7), the liquid inlet pipe (6) is communicated with the plurality of liquid storage boxes (7), the right end of the liquid inlet pipe (6) penetrates through the inner wall of the box (1) and is communicated with a liquid outlet pipe (8), the top of each of the plurality of liquid storage boxes (7) is communicated with a connecting pipe (13), the top end of each of the plurality of connecting pipes (13) penetrates through the bottom of the layer plate (4) and is communicated with a gas suction pipe (9), the top of each of the plurality of gas suction pipes (9) is connected with the gas suction pipe (9), one end of the gas suction pipe (9) penetrates through the inner wall of the box (1) and is communicated with a vacuum pump (12), the outer wall top end of each of the plurality of connecting pipes (13) is installed with an electromagnetic valve (10), the outer wall top end of each of the plurality of connecting pipes (13) is communicated with a microfluidic channel (11).
2. A cell recovery adapter according to claim 1, wherein: The sealing mechanism comprises a top cover (14), the bottom of the top cover (14) is fixedly connected with the top of the box (1), a plurality of taking openings (15) are formed in the top of the top cover (14), the top of the top cover (14) is rotatably connected with a plurality of rotating shafts (16), the outer wall of each of the plurality of rotating shafts (16) is rotatably connected with a sealing plate (17), the outer wall of each of the plurality of sealing plates (17) is fixedly connected with a rubber ring (19), the front side of each of the plurality of sealing plates (17) is formed with a clamping groove (18), the top front side of the top cover (14) is formed with a plurality of sliding grooves (20), the inside of each of the plurality of sliding grooves (20) is slidably connected with a sliding block (21), the front side of each of the plurality of sliding blocks (21) is fixedly connected with a spring (23), and the rear side of each of the plurality of sliding blocks (21) is fixedly connected with a clamping plate (22).
3. A cell recovery adapter according to claim 2, wherein: The sealing mechanism further comprises a plurality of buckling plates (2), and the bottom of each of the plurality of buckling plates (2) is fixedly connected with the top of the sliding block (21).
4. A cell recovery adapter according to claim 1, wherein: The ejection mechanism comprises a plurality of electric push rods (34), the bottom of each of the plurality of electric push rods (34) is fixedly connected with the inside bottom of the incubation cavity (3), one end of each of the plurality of electric push rods (34) is fixedly connected with a top plate (31), the bottom of each of the plurality of layer plates (4) is communicated with a placing cylinder (5), and the outer wall of the top plate (31) is slidably connected with the inner wall of the placing cylinder (5).
5. A cell recovery adapter according to claim 1, wherein: The adjusting mechanism comprises a plurality of humidity sensors (33), the plurality of humidity sensors (33) are fixedly connected to the inner wall of the cultivation cavity (3), the front side of the box (1) is provided with a liquid storage cylinder (24), the top of the liquid storage cylinder (24) is communicated with an atomizer (25), the top of the atomizer (25) is connected with an atomizing pipe (26), and the rear end of the atomizing pipe (26) penetrates through the front side of the box (1) and is communicated with an atomizing nozzle (27).
6. A cell recovery adapter according to claim 5, wherein: The outer wall of the liquid storage cylinder (24) is fixedly connected with a reinforcing plate (28), and the rear side of the two reinforcing plates (28) is fixedly connected to the front side of the box (1).
7. A cell recovery adapter according to claim 1, wherein: The front side of the box (1) is fixedly connected with a controller (29), and the inner wall of the plurality of cultivation cavities (3) is fixedly connected with a temperature sensor (32).
8. A cell recovery adapter according to claim 7, wherein: The controller (29) is electrically connected with the electromagnetic valve (10), the vacuum pump (12), the atomizer (25), the humidity sensor (33) and the temperature sensor (32) respectively, and the top of the controller (29) is fixedly connected with a display screen (30).