Self-cleaning cell selecting and inoculating equipment

Abstract

The utility model discloses self-cleaning type cell selecting and inoculating equipment, which relates to the technical field of cell cloning, and comprises a selecting mechanism and a plurality of tungsten needle components, a driving motor drives a turntable and the plurality of tungsten needle components to rotate, a selecting servo push rod and an inoculating servo push rod sequentially press the tungsten needle components downwards, and the tungsten needle components are separated from the selecting mechanism. The tungsten needle is used for dipping cells needing to be inoculated from the selection culture dish and transferring the cells into the inoculation culture dish, so that the cells are automatically selected and inoculated, an experimenter can conveniently select the cells, and the efficiency of selecting and inoculating the cells is improved; the cleaning assembly, the heating assembly, the alcohol dipping assembly, the fan and the cooling assembly can conduct automatic cleaning, high-temperature heating, alcohol disinfection and cooling on the tungsten needle, repeated use is convenient, the tungsten needle sleeve and the heat insulation rod are both made of ceramic materials, ceramic has the advantages of being resistant to high temperature and low in heat conductivity coefficient, and the guide shaft and the bearing seat are prevented from being damaged due to long-term high temperature; and the overall use effect and the service life of the tungsten needle assembly are improved.

Description

Technical Field

[0001] This utility model relates to the field of cell cloning technology, and in particular to a self-cleaning cell selection and inoculation device. Background Technology

[0002] Cloning refers to the asexual reproduction of an organism through somatic cells, and the offspring individuals with identical genotypes formed through asexual reproduction. The amazing cloning technology has shown broad application prospects, which can be summarized into the following four aspects: breeding superior livestock breeds and producing laboratory animals, producing transgenic animals, producing human embryonic stem cells for cell and tissue replacement therapy, replicating endangered animal species, preserving and disseminating animal species resources, and maintaining ecological balance. In the cloning process, it is necessary to pick out the cell clusters that have been cultured to a certain stage from one culture dish and inoculate them into another culture dish. The current conventional practice is to manually dip the tip of a toothpick into the cell cluster and then smear it into the other culture dish. In order to avoid cross-contamination, the toothpick needs to be replaced when inoculating another cell cluster.

[0003] For example, Chinese patent CN212476684U discloses a single-cell selection device, which includes a pipette, a camera component, a display screen, and an oral pipette. The pipette is provided with a pipetting port and a handle. The oral pipette includes a connecting part, a reservoir part, and an aspiration end. The connecting part of the oral pipette is connected to the pipetting port of the pipette. The camera component and the display screen are both mounted on the handle. The camera component is communicatively connected to the display screen. The focusing position of the camera component is the aspiration end of the oral pipette.

[0004] However, in existing technologies, cell clusters are inoculated by dipping toothpicks. Since the cell clusters to be inoculated are generally only 1 or 2 mm in size and are located in a large area of ​​culture material, the error of manual selection is large. At the same time, if an automatic cloning selection device is used, the irregular shape and large individual differences of toothpicks make it very difficult to feed and clamp them. Even if they are clamped, the position is not accurate, making it difficult to continue subsequent actions. In addition, the clamping time is time-consuming, resulting in low efficiency. To address the above problems, a self-cleaning cell selection and inoculation device is proposed. Utility Model Content

[0005] The purpose of this invention is to solve the problems existing in the prior art by proposing a self-cleaning cell selection and inoculation device.

[0006] To achieve the above objectives, this utility model adopts the following technical solution: a self-cleaning cell selection and inoculation device, comprising a selection mechanism and multiple tungsten needle assemblies. The selection mechanism includes a support plate, a drive motor, a heating assembly, an alcohol-dipping assembly, a fan, a turntable, a selection culture dish, an inoculation culture dish, a cooling assembly, and a base plate. An alcohol-dipping servo push rod, a selection servo push rod, and an inoculation servo push rod are fixedly installed on the top of the support plate. The output end of the drive motor is fixedly connected to the top of the turntable. The bottom of the support plate is fixedly installed to the top of the base plate. The heating assembly... The bottoms of the alcohol-dipping assembly and the cooling assembly are both fixedly installed to the top of the base plate. A cleaning assembly is fixedly installed at one end of the top of the base plate. The tungsten needle assembly includes a top block, a guide shaft, a bearing seat, a heat insulation ring, and a tungsten needle sleeve. The top of the guide shaft is fixedly installed to the bottom of the top block, and the bottom end of the guide shaft passes through the bearing seat and is fixedly connected to the top of the heat insulation ring. A heat insulation rod is fixedly connected inside the tungsten needle sleeve, and a tungsten needle is fixedly connected to the bottom of the tungsten needle sleeve. A spring is provided on the outer wall of the top of the guide shaft, and the outer edge of the top of the bearing seat is fixedly installed to the top of the turntable.

[0007] Preferably, the bottom of the heat insulation rod is fixedly connected to the top of the tungsten needle, and the bottom end of the heat insulation ring is fixedly connected to the top end of the tungsten needle sleeve.

[0008] Preferably, the heating component has a heating groove at the top, and the bottom of the fan is fixedly installed to the top of the base plate.

[0009] Preferably, two longitudinal servo linear modules are symmetrically fixedly installed at both ends of the top of the base plate, and a transverse servo linear module is installed on the top of the two longitudinal servo linear modules.

[0010] Preferably, each of the two lateral servo linear modules is equipped with a movable plate on its top, with the selection culture dish placed on one of the movable plates and the inoculation culture dish placed on the other movable plate.

[0011] Preferably, one end of the fan is fixedly connected to the bottom end of the cooling assembly.

[0012] Compared with the prior art, the advantages and positive effects of this utility model are as follows:

[0013] 1. In this invention, a selection culture dish containing cells that have been cultured for a period of time uses a separate 3D vision system to determine the cell growth status and accurate position. The selection culture dish is placed on a moving plate, and a new inoculation culture dish is placed on another moving plate. A two-dimensional platform composed of a longitudinal servo linear module and a transverse servo linear module automatically adjusts the position of the selection culture dish on the moving plate, moving the cells to be selected below the tungsten needle at the selection position. Another two-dimensional platform automatically adjusts the position of the inoculation culture dish on the moving plate, so that the inoculation point is located below the tungsten needle at the inoculation position. The device drives a turntable and multiple tungsten needle assemblies to rotate via a drive motor. When the tungsten needle assembly rotates to the bottom of the selection servo push rod, the selection servo push rod applies downward pressure, and the tungsten needle picks up the cells to be inoculated from the selection culture dish. When the tungsten needle assembly moves to the bottom of the inoculation servo push rod, the inoculation servo push rod applies downward pressure, and the tungsten needle moves downward and inoculates the cells into the inoculation culture dish, thereby automatically completing the cell selection and inoculation. This facilitates cell selection for laboratory personnel, has a high degree of automation, and improves the efficiency of cell selection and inoculation.

[0014] 2. In this utility model, by adding a cleaning component, a heating component, an alcohol-dipping component, a fan, and a cooling component, the tungsten needle is rinsed with water by the cleaning component, and then passes through the heating component, which can heat the tungsten needle to 300-400°C to remove residual cells. When the tungsten needle passes the top of the alcohol-dipping component, it is dipped in the alcohol, achieving the effect of sterilization. At the same time, the alcohol evaporates quickly, which can quickly cool the tungsten needle. When the tungsten needle passes through the air outlet of the cooling component, the fan can blow cooling gas from the air outlet of the cooling component onto the tungsten needle, thereby further cooling the tungsten needle to room temperature. Thus, the tungsten needle that has been inoculated with cells is automatically cleaned, sterilized by high-temperature heating, and cooled in sequence, which is convenient for repeated use.

[0015] 3. In this utility model, by setting a tungsten needle assembly, the tungsten needle of the tungsten needle assembly is made of tungsten steel, and the tungsten needle sleeve and heat insulation rod are made of ceramic. Ceramic has the functions of high temperature resistance and slow heat conduction. The tungsten needle sleeve, heat insulation rod and heat insulation ring are combined. When the tungsten needle is heated and sterilized at high temperature, it can effectively prevent the high temperature from being transferred upward to the guide shaft and bearing seat, greatly reduce heat conduction, avoid the guide shaft and bearing seat from being damaged by high temperature for a long time, and improve the overall use effect and service life of the tungsten needle assembly. Attached Figure Description

[0016] Figure 1 This invention provides a three-dimensional structural diagram of a self-cleaning cell selection and inoculation device;

[0017] Figure 2 This invention provides a schematic diagram of the rear structure of a self-cleaning cell selection and inoculation device;

[0018] Figure 3 This invention provides a schematic diagram of the selection mechanism of a self-cleaning cell selection and inoculation device;

[0019] Figure 4 This invention provides a schematic diagram of the structure of a tungsten needle assembly for a self-cleaning cell selection and inoculation device;

[0020] Figure 5 A front cross-sectional view of the tungsten needle assembly of a self-cleaning cell selection and inoculation device is provided for this utility model.

[0021] Legend: 1. Selection mechanism; 2. Tungsten needle assembly; 11. Support plate; 12. Alcohol-dipped servo push rod; 13. Selection servo push rod; 14. Drive motor; 15. Inoculation servo push rod; 16. Heating assembly; 17. Heating tank; 18. Alcohol-dipped assembly; 19. Fan; 110. Turntable; 111. Longitudinal servo linear module; 112. Lateral servo linear module; 113. Selection petri dish; 114. Inoculation petri dish; 115. Cooling assembly; 116. Base plate; 117. Moving plate; 118. Cleaning assembly; 21. Top block; 22. Guide shaft; 23. Spring; 24. Bearing seat; 25. Heat insulation ring; 26. Tungsten needle sleeve; 27. Tungsten needle; 28. Heat insulation rod. Detailed Implementation

[0022] To better understand the above-mentioned objectives, features, and advantages of this utility model, the present utility model will be further described below with reference to the accompanying drawings and embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0023] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the present invention is not limited to the specific embodiments disclosed in the following specification.

[0024] Example 1: As Figure 1 - Figure 5As shown, this utility model provides a self-cleaning cell selection and inoculation device, including a selection mechanism 1 and multiple tungsten needle assemblies 2. The selection mechanism 1 includes a support plate 11, a drive motor 14, a heating assembly 16, an alcohol-dipping assembly 18, a fan 19, a turntable 110, a selection culture dish 113, an inoculation culture dish 114, a cooling assembly 115, and a base plate 116. An alcohol-dipping servo push rod 12, a selection servo push rod 13, and an inoculation servo push rod 15 are fixedly installed on the top of the support plate 11. The output end of the drive motor 14 is fixedly connected to the top of the turntable 110. The bottom of the support plate 11 is fixedly installed to the top of the base plate 116. The tungsten needles... Component 2 includes a top block 21, a guide shaft 22, a bearing seat 24, a heat insulation ring 25, and a tungsten needle sleeve 26. The top of the guide shaft 22 is fixedly installed to the bottom of the top block 21, and the bottom end of the guide shaft 22 passes through the bearing seat 24 and is fixedly connected to the top of the heat insulation ring 25. A heat insulation rod 28 is fixedly connected inside the tungsten needle sleeve 26, and a tungsten needle 27 is fixedly connected to the bottom of the tungsten needle sleeve 26. A spring 23 is provided on the outer wall of the top of the guide shaft 22. The outer edge of the top of the bearing seat 24 is fixedly installed to the top of the turntable 110. The bottom of the heat insulation rod 28 is fixedly connected to the top of the tungsten needle 27, and the bottom end of the heat insulation ring 25 is fixedly connected to the top of the tungsten needle sleeve 26.

[0025] The specific settings and functions of this embodiment are described below: After the positions of the selection culture dish 113 and the inoculation culture dish 114 are adjusted, the selection mechanism 1 is activated by the controller, and the drive motor 14 drives the turntable 110 and multiple tungsten needle assemblies 2 to rotate (e.g., Figure 3 As shown, the turntable 110 rotates clockwise. When the tungsten needle assembly 2 rotates with the turntable 110 to the bottom of the selection servo push rod 13, the selection servo push rod 13 applies downward pressure, pressing down on the top block 21 of the tungsten needle assembly 2. The top block 21, guide shaft 22, tungsten needle sleeve 26, and tungsten needle 27 move downward. The end of the tungsten needle 27 is inserted into the selection culture dish 113. After the cells to be seeded are picked up by the tungsten needle 27, the selection servo push rod 13 stops applying pressure, and the top block 21 and guide shaft 22 automatically return to their original positions under the action of the spring 23. At this point, the drive motor 14 continues to drive the turntable 110 to rotate. When the turntable 110 moves the tungsten needle assembly 2 to the bottom of the inoculation servo push rod 15, the inoculation servo push rod 15 applies downward pressure, pressing down the top block 21 of the tungsten needle assembly 2. The tungsten needle 27 moves downward and inoculates the cells into the inoculation culture dish 114. Thus, through the combination of the selection mechanism 1 and the tungsten needle assembly 2, the selection and inoculation of cells are automatically completed, which is convenient for experimental personnel to select and clone cells. Moreover, the degree of automation is high, which improves the efficiency of cell selection and inoculation.

[0026] By improving the tungsten needle assembly 2, the guide shaft 22 of the tungsten needle assembly 2 is made of steel, the tungsten needle 27 is made of tungsten steel, and the tungsten needle sleeve 26 and the heat insulation rod 28 are both made of ceramic. Ceramic has the functions of high temperature resistance and slow thermal conductivity. The combination of the tungsten needle sleeve 26, the heat insulation rod 28 and the heat insulation ring 25 can effectively prevent the high temperature from being transferred upward to the guide shaft 22 and the bearing seat 24 when the tungsten needle 27 is heated and sterilized at high temperature. This greatly reduces heat conduction and prevents the guide shaft 22 and the bearing seat 24 from being damaged by high temperature for a long time, thereby improving the overall performance and service life of the tungsten needle assembly 2.

[0027] Example 2: Figure 1 - Figure 3 As shown, the bottoms of the heating assembly 16, the alcohol-dipping assembly 18, and the cooling assembly 115 are all fixedly installed to the top of the base plate 116. The top of the heating assembly 16 has a heating groove 17. The bottom of the fan 19 is fixedly installed to the top of the base plate 116. A cleaning assembly 118 is fixedly installed at one end of the top of the base plate 116. Two longitudinal servo linear modules 111 are symmetrically fixedly installed at both ends of the top of the base plate 116. A transverse servo linear module 112 is installed on the top of the two longitudinal servo linear modules 111. A moving plate 117 is installed on the top of each of the two transverse servo linear modules 112. The top of one moving plate 117 is fixedly connected to the bottom of the selection petri dish 113, and the top of the other moving plate 117 is fixedly connected to the bottom of the inoculation petri dish 114. One end of the fan 19 is fixedly connected to the bottom of the cooling assembly 115.

[0028] The overall effect of this embodiment is that the cells to be selected are placed in the selection culture dish 113, the seeding culture dish 114 is used to hold the selected cells, and the selection culture dish 113 is fixed to the top of the left movable plate 117 (e.g., Figure 3 (As shown), then fix the inoculation culture dish 114 to the top of the right-side movable plate 117 (as shown). Figure 3 As shown), the longitudinal servo linear module 111 can drive the transverse servo linear module 112 and the moving plate 117 to move back and forth as a whole, and the transverse servo linear module 112 can drive the moving plate 117 to move left and right as a whole. The longitudinal servo linear module 111 and the transverse servo linear module 112 can adjust the position of the selection culture dish 113 and the inoculation culture dish 114, so as to facilitate the selection of cells in the selection culture dish 113 and the inoculation of the selected cells to specific positions in the inoculation culture dish 114.

[0029] After cell selection and inoculation, the inoculation servo push rod 15 stops applying pressure, and the top block 21 and guide shaft 22 automatically return to their original positions under the action of spring 23. The drive motor 14 continues to drive the turntable 110 to rotate. The tungsten needle 27, after being inoculated with cells, passes sequentially through the cleaning assembly 118, the heating tank 17 of the heating assembly 16, the alcohol dipping assembly 18, and the air outlet at the top of the cooling assembly 115. The tungsten needle 27 is rinsed with water by the cleaning assembly 118, and then heated in the heating tank 17 of the heating assembly 16. The temperature is raised to 300-400℃ to remove residual cells. When the tungsten needle 27 passes the top of the alcohol-dipping component 18, it picks up the alcohol in the component, achieving the effect of sterilization. At the same time, the alcohol evaporates quickly, which can cool the tungsten needle rapidly. When the tungsten needle 27 passes the air outlet of the cooling component 115, the fan 19 can blow the cooling gas out from the air outlet of the cooling component 115, further cooling the tungsten needle to room temperature. Thus, the tungsten needle 27 that has been inoculated with cells is automatically cleaned, heated at high temperature, disinfected with alcohol, and cooled in sequence, which is convenient for repeated use.

[0030] The device is used as follows: The selection culture dish 113, which contains cells that have been cultured for a period of time, determines the cell growth status and accurate position through another 3D vision system. The selection culture dish 113 is placed on a moving plate 117, and the new inoculation culture dish 114 is placed on another moving plate 117. The two-dimensional platform composed of the longitudinal servo linear module 111 and the transverse servo linear module 112 automatically adjusts the position of the selection culture dish 113 on the moving plate 117, moving the cells to be selected below the tungsten needle 27 at the selection position. The other two-dimensional platform automatically adjusts the position of the inoculation culture dish 114 on the moving plate so that the inoculation point is located below the tungsten needle 27 at the inoculation position.

[0031] The controller activates the selection mechanism 1, and the drive motor 14 drives the turntable 110 and multiple tungsten needle assemblies 2 to rotate. When the tungsten needle assembly 2 rotates with the turntable 110 to the bottom of the selection servo push rod 13, the selection servo push rod 13 applies downward pressure, and the end of the tungsten needle 27 is inserted into the selection culture dish 113. After the tungsten needle 27 picks up the cells to be inoculated, the drive motor 14 continues to drive the turntable 110 to rotate. When the turntable 110 moves the tungsten needle assembly 2 to the bottom of the inoculation servo push rod 15, the inoculation servo push rod 15 applies downward pressure, pressing down the top block 21 of the tungsten needle assembly 2. The tungsten needle 27 moves downward and inoculates the cells into the inoculation culture dish 114. Thus, through the combination of the selection mechanism 1 and the tungsten needle assembly 2, the selection and inoculation cloning of cells are automatically completed, making it convenient for experimental personnel to select and clone cells.

[0032] After cell selection and inoculation, the inoculation servo push rod 15 stops applying pressure, and the top block 21 and guide shaft 22 automatically return to their original positions under the action of spring 23. The drive motor 14 continues to drive the turntable 110 to rotate. The tungsten needle 27, after being inoculated with cells, passes sequentially through the cleaning assembly 118, the heating tank 17 of the heating assembly 16, the alcohol dipping assembly 18, and the air outlet at the top of the cooling assembly 115. The tungsten needle 27 is rinsed with water by the cleaning assembly 118, and then heated in the heating tank 17 of the heating assembly 16. The temperature is raised to 300-400℃ to remove residual cells. When the tungsten needle 27 passes the top of the alcohol-dipping component 18, it picks up the alcohol in the component, achieving the effect of sterilization. At the same time, the alcohol evaporates quickly, which can cool the tungsten needle rapidly. When the tungsten needle 27 passes the air outlet of the cooling component 115, the fan 19 can blow the cooling gas out from the air outlet of the cooling component 115, further cooling the tungsten needle to room temperature. Thus, the tungsten needle 27 that has been inoculated with cells is automatically cleaned, heated at high temperature, disinfected with alcohol, and cooled in sequence, which is convenient for repeated use.

[0033] Meanwhile, by improving the tungsten needle assembly 2, both the tungsten needle sleeve 26 and the heat insulation rod 28 are made of ceramic material. Ceramic has the functions of high temperature resistance and slow heat conduction. When the tungsten needle 27 is heated and sterilized at high temperature, it can effectively prevent the high temperature from being transferred upward to the guide shaft 22 and the bearing seat 24, thereby improving the overall performance and service life of the tungsten needle assembly 2.

[0034] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model in any other way. Any person skilled in the art may make changes or modifications to the above-disclosed technical content to create equivalent embodiments for application in other fields. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present utility model without departing from the technical solution of the present utility model shall still fall within the protection scope of the present utility model.

Claims

1. A self-cleaning cell picking and inoculation apparatus, characterized by, The utility model provides a kind of picking mechanism (1) and multiple tungsten needle assemblies (2), the picking mechanism (1) includes support plate (11), driving motor (14), heating assembly (16), alcohol dipping assembly (18), fan (19), carousel (110), picking culture dish (113), inoculation culture dish (114), cooling assembly (115) and bottom plate (116), the top of the support plate (11) is fixedly installed with alcohol dipping servo push rod (12), picking servo push rod (13) and inoculation servo push rod (15), the output of the driving motor (14) is fixedly connected with the top of carousel (110), the bottom of the support plate (11) is fixedly installed with the top of bottom plate (116), the bottom of heating assembly (16), alcohol dipping assembly (18) and cooling assembly (115) is fixedly installed with the top of bottom plate (116), the top of bottom plate (116) one end is fixedly installed with cleaning assembly (118), the tungsten needle assembly (2) includes top block (21), guide shaft (22), bearing seat (24), heat insulation ring (25) and tungsten needle sleeve (26), the top of guide shaft (22) is fixedly installed with the bottom of top block (21), and the bottom end of the guide shaft (22) is fixedly connected with the top end of heat insulation ring (25) with bearing seat (24), heat insulation rod (28) is fixedly connected in the tungsten needle sleeve (26), and tungsten needle (27) is fixedly connected in the bottom of the tungsten needle sleeve (26), the top end outer wall of guide shaft (22) is provided with spring (23), the top end outer edge of bearing seat (24) is fixedly installed with the top end of carousel (110).

2. The self-cleaning cell plucking and inoculation apparatus according to claim 1, wherein: The bottom of the heat insulation rod (28) is fixedly connected with the top of the tungsten needle (27), and the bottom end of the heat insulation ring (25) is fixedly connected with the top end of the tungsten needle sleeve (26).

3. The self-cleaning cell plucking and seeding apparatus according to claim 1, wherein: The top of the heating assembly (16) is provided with a heating groove (17), and the bottom end of the fan (19) is fixedly installed with the top of the bottom plate (116).

4. The self-cleaning cell plucking and seeding apparatus according to claim 1, wherein: Two longitudinal servo linear modules (111) are symmetrically fixedly installed at both ends of the top of the bottom plate (116), and the top of each of the two longitudinal servo linear modules (111) is provided with a transverse servo linear module (112).

5. The self-cleaning cell plucking and seeding apparatus according to claim 4, wherein: The top of each of the two transverse servo linear modules (112) is provided with a moving plate (117), and the picking culture dish (113) is placed on one of the moving plates (117), and the inoculation culture dish is placed on the other moving plate (117).

6. The self-cleaning cell plucking seeding apparatus according to claim 1, wherein: One end of the fan (19) is fixedly connected with the bottom end of the cooling assembly (115).

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