Car-t cell culture dish

Abstract

The utility model relates to the technical field of cell culture dishes, in particular to a car-t cell culture dish which comprises a cylindrical tube body, two ends of the cylindrical tube body are in opening type design, and an external thread is arranged on the outer wall of one end of the cylindrical tube body; the cylindrical pipe body is connected with a sealing bottom cover, the sealing bottom cover is provided with an embedded face tightly attached to the inner wall of the cylindrical pipe body, and a screw joint groove matched with the outer threads is formed in the sealing bottom cover. A sealing top cover is connected to the cylindrical pipe body; by arranging the cylindrical tube body with openings at two ends, and the sealing top cover and the sealing bottom cover which are respectively matched with the cylindrical tube body, the combined type design of the culture dish is realized, so that the culture dish can be respectively disassembled during cleaning and disinfection, the existence of an included angle between the tube body and the tube wall is avoided, and compared with a traditional culture dish with a cover, the culture dish is more convenient to use. The culture dish is more convenient and quicker to clean and disinfect, and is beneficial to improving the cleaning efficiency.

Description

Technical Field

[0001] This utility model relates to the field of cell culture dish technology, specifically a CAR-T cell culture dish. Background Technology

[0002] CAR-T therapy, or chimeric antigen receptor T-cell immunotherapy, is a novel precision targeted therapy for treating tumors. In recent years, through optimization and improvement, it has achieved good results in clinical tumor treatment. It is a very promising new tumor immunotherapy method that can be precise, rapid, efficient, and potentially curative for cancer.

[0003] CAR-T cells need to be cultured and obtained. The existing culture dishes are colorless and transparent acrylic round boxes with lids. These culture dishes are not only simple and practical, but also convenient for researchers to observe and record. During the culture process, staff need to clean and disinfect the culture dishes after use to avoid bacteria affecting the normal culture of cells. However, during the cleaning process, because there is an angle between the bottom of the culture dish and the side wall of the box, researchers need to use a brush to repeatedly brush the dish, which is time-consuming and has low cleaning efficiency.

[0004] Therefore, we propose a CAR-T cell culture dish. Utility Model Content

[0005] One of the technical problems this application aims to solve is that, in the current cleaning process, because there is an angle between the bottom of the culture dish and the side wall of the dish, researchers need to use a brush to repeatedly brush the dish, which is time-consuming and has low cleaning efficiency.

[0006] To address the aforementioned technical problems, this application provides a CAR-T cell culture dish, comprising a cylindrical tube with open ends and an external thread on the outer wall of one end. A sealing bottom cover is connected to the cylindrical tube, having an embedded surface that fits tightly against the inner wall of the cylindrical tube, and a threaded groove that mates with the external thread. A sealing top cover is also connected to the cylindrical tube, its inner wall fitting tightly against the outer wall of the cylindrical tube. The top and bottom covers are located at opposite ends of the cylindrical tube. The dish further includes a connector and a limiting member. The connector is disposed on the top cover, and the limiting member is disposed on the bottom cover. The connector and the limiting member are connected to each other, thus fixing the top cover to the cylindrical tube.

[0007] In some embodiments, the outer wall of the sealing bottom cover is provided with an extension plate, the limiting member includes a plurality of L-shaped limiting plates disposed on the extension plate, and the connecting member includes a plurality of connecting plates disposed on the outer wall of the sealing top cover. The connecting plates correspond one-to-one with the L-shaped limiting plates, and the connecting plates can be tightly connected to the L-shaped limiting plates.

[0008] In some embodiments, a first anti-slip patch is provided on the top surface of the connecting plate, and a second anti-slip patch is provided on the mating surface of the L-shaped limiting plate and the connecting plate.

[0009] In some embodiments, a lubrication zone is provided on the top surface of the sealing top cover.

[0010] In some embodiments, uniformly distributed anti-slip protrusions are provided on the outer wall of the sealing bottom cover.

[0011] In some embodiments, uniformly distributed anti-slip stripes are provided on the outer wall of the sealed top cover.

[0012] In some embodiments, the inner wall of the sealing top cover is provided with a chamfered wall.

[0013] In some embodiments, a positive magnetic sheet is provided on the top surface of the connecting plate, and a negative magnetic sheet is provided on the L-shaped limiting plate, wherein the positive magnetic sheet and the negative magnetic sheet are attracted to each other.

[0014] This utility model has at least the following beneficial effects:

[0015] 1. By designing a cylindrical tube with openings at both ends, and a sealing top cover and a sealing bottom cover that fit into the cylindrical tube respectively, a modular design for the petri dish is achieved. This allows for separate disassembly and processing during cleaning and sterilization, avoiding the presence of an angle between the tube and the tube wall. Compared with traditional covered petri dishes, this petri dish is more convenient and faster to clean and sterilize, which helps to improve cleaning efficiency.

[0016] 2. By setting up connecting plates and L-shaped limiting plates, after both the sealing top cover and the sealing bottom cover are installed on the cylindrical tube, the connecting plates and L-shaped limiting plates are tightly fitted by rotating the sealing top cover. The friction generated by the fit of multiple connecting plates and L-shaped limiting plates can force the sealing top cover to be stably limited, preventing the sealing top cover from loosening or falling off during the transfer of the culture dish, thus providing a safety guarantee for cell culture. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0018] Figure 2 This is a schematic diagram of the exploded three-dimensional structure of this utility model;

[0019] Figure 3for Figure 2 Axonometric three-dimensional structural schematic diagram;

[0020] Figure 4 This is a schematic diagram of the structure of Embodiment 2 of this utility model;

[0021] Figure 5 for Figure 4 Axonometric three-dimensional structural schematic diagram.

[0022] In the diagram: 1. Cylindrical tube; 2. Sealed top cover; 3. Sealed bottom cover; 4. Lubrication area; 5. Anti-slip stripes; 6. Extension plate; 7. Connector; 8. Limiting component; 9. Connecting plate; 10. L-shaped limiting plate; 11. Screw groove; 12. Embedding surface; 13. Anti-slip protrusion; 14. External thread; 15. Anti-slip patch No. 1; 16. Chamfered wall; 17. Anti-slip patch No. 2; 18. Positive magnetic sheet; 19. Negative magnetic sheet. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0024] Example 1:

[0025] Please see Figures 1 to 3 The technical solution provided by this utility model is as follows: A CAR-T cell culture dish includes a cylindrical tube 1, both ends of which are open. An external thread 14 is provided on the outer wall of one end of the cylindrical tube 1. A sealing bottom cover 3 is connected to the cylindrical tube 1. The sealing bottom cover 3 is provided with an embedded surface 12 that fits tightly against the inner wall of the cylindrical tube 1. A threaded groove 11 that mates with the external thread 14 is provided on the sealing bottom cover 3. A sealing top cover 2 is connected to the cylindrical tube 1. The inner wall of the sealing top cover 2 fits tightly against the outer wall of the cylindrical tube 1. The sealing top cover 2 and the sealing bottom cover 3 are respectively located at one end of the cylindrical tube 1. The dish also includes a connector 7 and a limiting member 8. The connector 7 is provided on the sealing top cover 2, and the limiting member 8 is provided on the sealing bottom cover 3. The connector 7 and the limiting member 8 are connected to limit and fix the sealing top cover 2 to the cylindrical tube 1.

[0026] During assembly, take the cylindrical tube 1 and the sealing bottom cover 3 respectively, and twist the sealing bottom cover 3 clockwise to connect it to the external thread 14. The outer wall of the embedding surface 12 fits tightly with the inner wall of the cylindrical tube 1. Then, place the CAR-T cells on the top of the embedding surface 12, and then cover it with the sealing top cover 2. The operation is simple.

[0027] In this embodiment, as Figure 2 and Figure 3 As shown, the outer wall of the sealing bottom cover 3 is provided with an extension plate 6, the limiting member 8 includes multiple L-shaped limiting plates 10 provided on the extension plate 6, and the connecting member 7 includes multiple connecting plates 9 provided on the outer wall of the sealing top cover 2. The connecting plate 9 corresponds one-to-one with the L-shaped limiting plate 10, and the connecting plate 9 can be tightly connected with the L-shaped limiting plate 10. A first anti-slip patch 15 is provided on the top surface of the connecting plate 9, and a second anti-slip patch 17 is provided on the contact surface between the L-shaped limiting plate 10 and the connecting plate 9. After the sealing top cover 2 is installed, press your hand on the extension plate 6 to stabilize the cylindrical tube 1, and slowly rotate the sealing top cover 2 to move the connecting plate 9 to fit with the L-shaped limiting plate 10, thereby limiting the sealing top cover 2.

[0028] In this embodiment, as Figure 1 and Figure 2 As shown, a lubrication area 4 is provided on the top surface of the sealed top cover 2. The surface of the lubrication area 4 is relatively smooth and can be used to attach labels to indicate the inspection period or information of the petri dish.

[0029] In this embodiment, as Figure 1 and Figure 2 As shown, the outer wall of the sealing bottom cover 3 is provided with evenly distributed anti-slip protrusions 13. This is designed to increase friction and facilitate the screwing and unscrewing of the sealing bottom cover 3.

[0030] In this embodiment, as Figure 1 and Figure 2 As shown, uniformly distributed anti-slip stripes 5 are provided on the outer wall of the sealing top cover 2. This is designed to increase friction and facilitate the installation or removal of the sealing top cover 2.

[0031] In this embodiment, as Figure 3 As shown, the inner wall of the sealing top cover 2 is provided with a chamfered wall 16. The chamfered wall 16 increases the included angle of the inner wall of the sealing top cover 2, making it easier for cleaning personnel to pry their fingers in and making cleaning easier.

[0032] The implementation principle of a CAR-T cell culture dish in this application embodiment is as follows: During assembly, take the cylindrical tube 1 and the sealing bottom cover 3 respectively, and twist the sealing bottom cover 3 clockwise to connect it to the external thread 14. The outer wall of the embedding surface 12 is tightly attached to the inner wall of the cylindrical tube 1. Then, place the CAR-T cells on the top of the embedding surface 12, and cover it with the sealing top cover 2. After the sealing top cover 2 is installed, press your hand on the extension plate 6 to stabilize the cylindrical tube 1, and slowly rotate the sealing top cover 2 to move the connecting plate 9 to fit with the L-shaped limiting plate 10, thereby limiting the sealing top cover 2.

[0033] Example 2:

[0034] Please see Figure 4 and Figure 5 The technical solution provided by this utility model is as follows:

[0035] Unlike Embodiment 1, the top surface of the connecting plate 9 is provided with a positive magnetic sheet 18, and the L-shaped limiting plate 10 is provided with a negative magnetic sheet 19. The positive magnetic sheet 18 and the negative magnetic sheet 19 are attracted to each other, and the limiting is achieved by the attraction between the positive and negative magnetic sheets. This not only increases the connection stability between the cylindrical tube 1 and the sealing top cover 2, but also reduces the friction during installation or disassembly, reduces wear, and extends the service life.

[0036] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0037] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention.

Claims

1. A car-t cell culture dish comprising a cylindrical tube body (1), characterized in that: Both ends of the cylindrical pipe body (1) are open design, located in one end of the cylindrical pipe body (1) outer wall is provided with external thread (14); The cylindrical pipe body (1) is connected with the sealing bottom cover (3), the sealing bottom cover (3) is provided with the embedded surface (12) which is closely combined with the inner wall of the cylindrical pipe body (1), the sealing bottom cover (3) is provided with the screw groove (11) matched with the external thread (14); The sealing top cover (2) is connected to the cylindrical pipe body (1), the inner wall of the sealing top cover (2) is closely combined with the outer wall of the cylindrical pipe body (1), and the sealing top cover (2) and the sealing bottom cover (3) are located at one end of the cylindrical pipe body (1) respectively; It also includes a connecting piece (7) and a limiting piece (8); The connecting piece (7) is arranged on the sealing top cover (2), the limiting piece (8) is arranged on the sealing bottom cover (3), and the connecting piece (7) and the limiting piece (8) are connected, so that the sealing top cover (2) and the cylindrical pipe body (1) are limited and fixed.

2. The car-t cell culture dish of claim 1, wherein: The outer wall of the sealing bottom cover (3) is provided with an extension plate (6), the limiting piece (8) includes a plurality of L-shaped limiting plates (10) arranged on the extension plate (6), the connecting piece (7) includes a plurality of connecting plates (9) arranged on the outer wall of the sealing top cover (2), the connecting plate (9) corresponds to the L-shaped limiting plate (10) one by one, and the connecting plate (9) can be closely connected with the L-shaped limiting plate (10).

3. The car-t cell culture dish of claim 2, wherein: A first anti-skid patch (15) is arranged on the top surface of the connecting plate (9), and a second anti-skid patch (17) is arranged on the abutting surface of the L-shaped limiting plate (10) and the connecting plate (9).

4. The car-t cell culture dish of claim 1, wherein: A lubricating area (4) is arranged on the top surface of the sealing top cover (2).

5. The car-t cell culture dish of claim 1, wherein: Uniformly distributed anti-skid protrusions (13) are arranged on the outer wall of the sealing bottom cover (3).

6. The car-t cell culture dish of claim 1, wherein: Uniformly distributed anti-skid stripes (5) are arranged on the outer wall of the sealing top cover (2).

7. The car-t cell culture dish of claim 1, wherein: The inner wall of the sealing top cover (2) is provided with a chamfer wall (16).

8. The car-t cell culture dish of claim 2, wherein: The top surface of the connecting plate (9) is provided with a positive magnetic sheet (18), and the L-shaped limiting plate (10) is provided with a negative magnetic sheet (19), and the positive magnetic sheet (18) and the negative magnetic sheet (19) are adsorbed.

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