Kit for real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection

Abstract

The utility model relates to the technical field of kits, in particular to a kit for real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection, which comprises a kit body, an object taking opening is formed in a top cover of the kit body, an easy-to-tear strip is arranged in the object taking opening and is matched with the top cover of the kit body through a slotted line, and a connecting component is arranged on the easy-to-tear strip and the top cover of the kit body together. The connecting assembly is used for detachably fixing the easy-to-tear strip to the inner side of the object taking opening. According to the kit, the plurality of fetching openings corresponding to the reagent tubes are formed in the top cover of the kit body, the fetching openings can be opened through the easy-to-tear strips in the fetching openings, and finally the fetching openings can be sealed by the easy-to-tear strips through the connecting assemblies, so that the whole top cover does not need to be opened when only one or more reagent tubes are fetched, and the operation is convenient. A connecting piece between the top cover and the box body is prevented from being damaged; and the protection function of the box body can be guaranteed through the adhesive part.

Description

Technical Field

[0001] This utility model relates to the field of reagent kit technology, specifically a reagent kit for real-time quantitative PCR detection. Background Technology

[0002] Real-time quantitative PCR is an important molecular biology technique widely used in gene expression analysis, pathogen detection, and genetic disease diagnosis. Real-time quantitative PCR detection kits provide researchers and clinicians with a variety of reagents to ensure the accuracy of experiments.

[0003] Existing real-time quantitative PCR detection kits typically consist of a paper box and a sponge plate for securing the reagent tubes. During packaging, multiple reagent tubes are placed into slots on the sponge plate to secure them. The sponge plate is then placed into the box, and the top cover of the box is closed to package the reagents. When the reagents are needed, the top cover can be flipped open, and the reagent tubes can be removed from the box.

[0004] However, in the above process, if staff only need to use one or a few reagents at a time, they must close the lid of the container after using the required reagents and secure it with the connector between the lid and the container to prevent the remaining reagents from falling out. Since both the container and lid are made of paper, repeated opening and closing may damage the connector, causing the container to fail to provide adequate protection for the reagent tubes. Therefore, we propose a real-time quantitative PCR detection kit to effectively address these shortcomings. Utility Model Content

[0005] The purpose of this invention is to provide a real-time fluorescence quantitative PCR detection kit to solve the problems mentioned in the background art.

[0006] This utility model is achieved through the following technical solution: a real-time fluorescence quantitative PCR detection kit, including a box body, a dispensing port on the top cover of the box body, an easy-tear strip inside the dispensing port, the easy-tear strip engaging with the top cover of the box body via a slotted line, and a connecting component shared by the easy-tear strip and the top cover of the box body, the connecting component being used to detachably fix the easy-tear strip to the inside of the dispensing port.

[0007] Optionally, the connecting assembly includes an adhesive part and two release papers. The adhesive part is fixedly connected to the bottom surface of the easy-tear strip, and the two release papers are respectively fixedly connected to the bottom surface of the top cover of the box. The opposing surfaces of the two release papers extend to the inside of the dispensing opening, and the portion of the release paper extending to the inside of the dispensing opening is adhered to the bottom surface of the adhesive part.

[0008] Optionally, the retrieval opening has a rectangular structure, and the easy-tear strip is located on one side inside the retrieval opening and is fixedly connected to the side wall of the retrieval opening. The other three sides of the easy-tear strip inside the retrieval opening are all fitted with the side wall of the retrieval opening through slotted lines.

[0009] Optionally, the upper surface of the easy-tear strip is provided with a force-bearing part that protrudes outward, and the force-bearing part is located at the movable end of the easy-tear strip.

[0010] Optionally, both the adhesive portion and the release paper are rectangular, the width of the adhesive portion is not greater than the width of the tear strip, and the length of the release paper is not less than the length of the adhesive portion.

[0011] Optionally, the number of retrieval ports is equal to the number of rows of reagent tubes inside the box, the width of the retrieval port is greater than the diameter of the reagent tube, and the retrieval port is located directly above the reagent tube inside the box.

[0012] Compared with the prior art, this utility model provides a real-time fluorescence quantitative PCR detection kit, which has the following beneficial effects:

[0013] This invention provides multiple dispensing ports corresponding to reagent tubes on the top cover of the box. The ports are opened by easy-tear strips inside the ports, and finally closed by connecting components. This design allows the entire top cover to be opened when only one or a few reagent tubes are being dispensed, thus avoiding damage to the connection between the top cover and the box. It also ensures the protective function of the box through adhesive parts. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the structure of this utility model;

[0015] Figure 2 This is a top view of the present invention;

[0016] Figure 3 This is a schematic diagram of the top cover of this utility model in the open state;

[0017] Figure 4 This is a schematic diagram of the disassembled structure of the easy-tear strip and connecting components of this utility model.

[0018] In the diagram: 1. Box body; 2. Dispensing opening; 3. Easy-tear strip; 301. Force-bearing part; 4. Connecting component; 401. Adhesive part; 402. Release paper. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] In existing reagent kits, the kit and the top cover are usually connected by a connector on the front face. The insert on the kit is inserted into the lower surface of the top cover to complete the connection with the top cover. When only one or a few reagent tubes are used each time, the top cover needs to be opened and closed multiple times to ensure that the reagents in the kit are not lost. However, the insert will be damaged during the process of repeatedly pulling out and inserting the insert. Over time, the connection between the insert and the top cover will become weak, affecting the protective function of the kit.

[0021] Please see Figure 1 - Figure 4 A real-time quantitative PCR detection kit includes a box body 1. The top cover of the box body 1 has a dispensing port 2. An easy-tear strip 3 is provided inside the dispensing port 2. The easy-tear strip 3 engages with the top cover of the box body 1 via a slotted line, allowing it to be torn open, thus opening the dispensing port 2 and enabling the user to retrieve the reagent tubes inside the box body 1. A connecting component 4 is provided on both the easy-tear strip 3 and the top cover of the box body 1. The connecting component 4 allows the easy-tear strip 3 to be detachably fixed inside the dispensing port 2, so that after being torn open, it can be resealed within the dispensing port 2 via the connecting component 4, thus re-sealing the dispensing port 2 and ensuring that the box body 1 still protects the reagent tubes.

[0022] Furthermore, the connecting component 4 includes an adhesive part 401 and two release papers 402. The adhesive part 401 is fixedly connected to the bottom surface of the easy-tear strip 3, and the two release papers 402 are respectively fixedly connected to the bottom surface of the top cover of the box body 1. The opposing surfaces of the two release papers 402 extend to the inside of the retrieval port 2. The portion of the release paper 402 extending to the inside of the retrieval port 2 is adhered to the bottom surface of the adhesive part 401, so that when the user tears the easy-tear strip 3 from the retrieval port 2, the adhesive part 401 will also be driven to tear the portion of the release paper 402 extending to the retrieval port 2. When the easy-tear strip 3 is pressed into the retrieval port 2 again, the adhesive part 401 at the bottom of the easy-tear strip 3 will adhere to the release paper 402 again, so that the easy-tear strip 3 will close the retrieval port 2 again.

[0023] Specifically, the dispensing opening 2 has a rectangular structure. An easy-tear strip 3 is located on one side of the dispensing opening 2 and is fixedly connected to the side wall of the dispensing opening 2. The other three sides of the easy-tear strip 3 inside the dispensing opening 2 are also fitted with the side wall of the dispensing opening 2 via slotted lines. This ensures that even after the easy-tear strip 3 is torn open, one end remains connected to the top cover of the box body 1, preventing the easy-tear strip 3 from being lost and thus ensuring the dispensing opening 2 can be sealed. It also improves the stability of the easy-tear strip 3 when closed. Furthermore, the user can adjust the degree to which the easy-tear strip 3 is torn according to the amount of reagent dispensed, thereby controlling the size of the opening of the dispensing opening 2.

[0024] To make it easier for users to tear the easy-tear strip 3, the upper surface of the easy-tear strip 3 of this application is provided with a force-receiving part 301 protruding outward. The force-receiving part 301 is located at the movable end of the easy-tear strip 3, so that users can pull the force-receiving part 301 upward to drive the movable end of the easy-tear strip 3 to move, thereby tearing the easy-tear strip 3 and making it easier for users to take out the reagents in the box 1.

[0025] In this embodiment, both the adhesive part 401 and the release paper 402 are rectangular. The width of the adhesive part 401 is not greater than the width of the easy-tear strip 3, to prevent the adhesive part 401 from protruding outside the easy-tear strip 3, thus preventing the adhesive part 401 from sticking to the box body 1 and avoiding damage to the box body 1. The length of the release paper 402 is not less than the length of the adhesive part 401, so that after the adhesive part 401 and the release paper 402 are attached, the easy-tear strip 3 can completely cover the dispensing opening 2. Specifically, when the user tears the easy-tear strip 3 open using the force-bearing part 301, the easy-tear strip 3 will cause the adhesive part 401 at the bottom to move upwards until it moves above the top cover of the box body 1, while the release paper 402 remains on the bottom surface of the top cover of the box body 1, partially exposed into the dispensing port 2; at this time, the dispensing port 2 is opened, and the user can take the reagent tube inside the box body 1; after use, the user can press the easy-tear strip 3 back into the dispensing port 2, so that the adhesive surface of the adhesive part 401 and the release paper 402 are bonded again, thereby fixing the easy-tear strip 3, and then closing the dispensing port 2 again to protect the reagent tube.

[0026] The number of retrieval ports 2 is equal to the number of rows of reagent tubes inside the box 1. The width of the retrieval ports 2 is greater than the diameter of the reagent tubes, and the retrieval ports 2 are located directly above the reagent tubes inside the box 1, so that users can retrieve the reagent tubes more conveniently and quickly.

[0027] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes the element.

[0028] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A real-time quantitative PCR detection kit, comprising a housing (1), characterized in that: The top cover of the box body (1) has an opening (2) for taking out items. The opening (2) has an easy-tear strip (3) inside. The easy-tear strip (3) is connected to the top cover of the box body (1) through a slotted line. The easy-tear strip (3) and the top cover of the box body (1) are provided with a connecting component (4). The connecting component (4) is used to make the easy-tear strip (3) detachably fixed to the inside of the opening (2).

2. The real-time fluorescence quantitative PCR detection kit according to claim 1, characterized in that: The connecting component (4) includes an adhesive part (401) and two release papers (402). The adhesive part (401) is fixedly connected to the bottom surface of the easy-tear strip (3). The two release papers (402) are respectively fixedly connected to the bottom surface of the top cover of the box body (1), and the opposing surfaces of the two release papers (402) extend to the inside of the retrieval port (2). The portion of the release paper (402) extending to the inside of the retrieval port (2) is adhered to the bottom surface of the adhesive part (401).

3. The real-time fluorescence quantitative PCR detection kit according to claim 1, characterized in that: The retrieval opening (2) has a rectangular structure. The easy-tear strip (3) is located on one side inside the retrieval opening (2) and is fixedly connected to the side wall of the retrieval opening (2). The other three sides of the easy-tear strip (3) inside the retrieval opening (2) are all fitted with the side wall of the retrieval opening (2) through slotted lines.

4. The real-time fluorescence quantitative PCR detection kit according to claim 3, characterized in that: The upper surface of the easy-tear strip (3) is provided with a force-bearing part (301) protruding outward, and the force-bearing part (301) is located at the movable end of the easy-tear strip (3).

5. The real-time fluorescence quantitative PCR detection kit according to claim 2, characterized in that: Both the adhesive part (401) and the release paper (402) are rectangular. The width of the adhesive part (401) is not greater than the width of the easy-tear strip (3), and the length of the release paper (402) is not less than the length of the adhesive part (401).

6. The real-time fluorescence quantitative PCR detection kit according to claim 1, characterized in that: The number of the extraction ports (2) is equal to the number of rows of reagent tubes inside the box (1). The width of the extraction port (2) is greater than the diameter of the reagent tube, and the extraction port (2) is located directly above the reagent tube inside the box (1).

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