Amylase multi-stage solid-state fermentation device

Abstract

The multistage solid-state amylase fermentation device comprises a fermentation tank, a partition plate is arranged in the fermentation tank, and an inner cavity of the fermentation tank is divided into an upper primary fermentation cavity and a lower deep fermentation cavity by the partition plate; an outer rotating shaft is arranged in the primary fermentation cavity, an inner rotating shaft is arranged in the outer rotating shaft, the lower end of the inner rotating shaft downwards penetrates through the partition plate and extends into the deep fermentation cavity, a first stirring blade is arranged on the outer wall of the outer rotating shaft, and a second stirring blade is arranged on the outer wall of the inner rotating shaft located in the deep fermentation cavity; the inner rotating shaft part located in the outer rotating shaft is in transmission fit with the outer rotating shaft through a planetary gear set, a gear motor is arranged at the top of the fermentation tank, and a driving shaft of the gear motor downwards extends into the fermentation tank and is connected with the upper end of the inner rotating shaft. By the adoption of the structure, the problems that an existing single-cavity fermentation tank is difficult to control and poor in fermentation efficiency and quality are solved.

Description

Technical Field

[0001] This utility model relates to the field of fermentation technology, specifically a multi-stage solid-state fermentation device for amylase. Background Technology

[0002] In recent years, solid-state fermentation has received increasing attention due to its advantages such as wide availability of raw materials, low cost, and environmental friendliness. Compared with liquid fermentation, solid-state fermentation has a lower moisture content, which helps reduce the amount of waste liquid generated during microbial growth and can also increase the concentration of the target product.

[0003] Currently, in traditional solid-state fermentation of amylase, fermenters typically employ a single-chamber structure, making precise control of the fermentation process difficult, resulting in low fermentation efficiency and unstable product quality. Furthermore, traditional fermentation devices struggle to effectively transfer heat during fermentation, easily causing localized overheating or undercooling, which negatively impacts fermentation outcomes. Therefore, improving fermentation efficiency and product quality has become a pressing issue in this field. Summary of the Invention

[0004] The technical problem to be solved by this utility model is to provide a multi-stage solid-state fermentation device for amylase, which solves the problems of difficult control, insufficient fermentation efficiency and quality of existing single-chamber fermenters.

[0005] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is: a multi-stage solid-state fermentation device for amylase, including a fermentation tank, wherein a partition is provided inside the fermentation tank, and the partition divides the internal cavity of the fermentation tank into an upper primary fermentation chamber and a lower deep fermentation chamber.

[0006] The primary fermentation chamber is provided with an outer rotating shaft, and an inner rotating shaft is provided inside the outer rotating shaft. The lower end of the inner rotating shaft passes downward through the partition and extends into the deep fermentation chamber. A first stirring blade is provided on the outer wall of the outer rotating shaft, and a second stirring blade is provided on the outer wall of the inner rotating shaft located in the deep fermentation chamber.

[0007] The inner rotating shaft portion located within the outer rotating shaft is connected to the outer rotating shaft via a planetary gear set. A reduction motor is provided at the top of the fermentation tank, and the drive shaft of the reduction motor extends downward into the fermentation tank and is connected to the upper end shaft of the inner rotating shaft.

[0008] In a preferred embodiment, the planetary gear set includes a sun gear mounted on an inner rotating shaft and planetary pinions mounted in multiple openings on the sidewall of an outer rotating shaft, wherein the sun gear meshes with the planetary pinions;

[0009] The fermenter has a vertical cylinder on its top surface and a gear ring on the inner wall of its lower end. The gear ring, the sun gear, and the planetary pinions are at the same horizontal level, and multiple planetary pinions mesh with the gear ring simultaneously.

[0010] In a preferred embodiment, the primary fermentation chamber has a feeding port on its side wall, and the deep fermentation chamber has a discharge port at its bottom.

[0011] The lower end of the inner rotating shaft extends into the discharge port, and the inner rotating shaft portion located inside the discharge port is provided with spiral blades.

[0012] In a preferred embodiment, a feeding adjustment plate is provided below the partition, the partition is provided with a plurality of first feeding holes arranged radially, and the feeding adjustment plate is provided with a plurality of second feeding holes arranged radially, wherein the shape and size of a single first feeding hole and a single second feeding hole are the same;

[0013] The partition is fixedly installed inside the fermentation tank, and the feeding adjustment plate is movably installed below the partition and fits against the partition. The feeding adjustment plate can rotate around its own center.

[0014] In a preferred embodiment, a protrusion is provided on one side of the feeding adjustment plate, and the protrusion extends outside the fermentation tank.

[0015] In a preferred embodiment, an adjusting vertical rod is provided on the protrusion outside the fermentation tank, and a fixing block is provided on the outer wall of the fermentation tank above the protrusion. The fixing block has an arc-shaped hole, and the adjusting vertical rod is set to pass through the arc-shaped hole upward.

[0016] In a preferred embodiment, both the primary fermentation chamber and the deep fermentation chamber are provided with heat exchange jackets on their outer walls, and the two heat exchange jackets are connected by a connecting pipe.

[0017] The heat exchange jacket on the outer wall of the deep fermentation chamber has a heat exchange medium inlet pipe at the bottom edge of the side wall, and the heat exchange jacket on the outer wall of the primary fermentation chamber has a heat exchange medium outlet pipe at the top edge of the side wall.

[0018] In a preferred embodiment, a heat exchange medium delivery pump is provided on the heat exchange medium inlet pipe;

[0019] Temperature sensors are installed on the side walls of both the primary fermentation chamber and the deep fermentation chamber. The two temperature sensors are connected to the controller, which is connected to the heat exchange medium transfer pump and the geared motor.

[0020] The amylase multi-stage solid-state fermentation device provided by this utility model, by adopting the above-described structure, has the following beneficial effects:

[0021] (1) The dual-shaft stirring system (outer and inner shafts) used in the device, combined with the planetary gear transmission mechanism, not only ensures that the materials in the primary fermentation chamber and the deep fermentation chamber are fully and evenly stirred, but also realizes the stirring requirements of different intensities between the two chambers, which is conducive to the growth and reproduction of microorganisms and the generation of metabolites.

[0022] (2) By setting the heat exchange jacket outside the fermenter and its supporting control system, the temperature change during the entire fermentation process can be precisely controlled, which helps to maintain the best fermentation environment, promote the maximum expression of target enzyme activity, and reduce the risk of product quality decline due to temperature fluctuations.

[0023] (3) The adjustable feeding mechanism allows operators to flexibly adjust the speed and amount of material transitioning from the initial fermentation stage to the deep fermentation stage according to actual needs, thereby better matching the changes in the characteristics of different batches of raw materials or process requirements, and improving the overall fermentation efficiency and the stability of the final product. Attached Figure Description

[0024] The present invention will be further described below with reference to the accompanying drawings and embodiments:

[0025] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0026] Figure 2 This is a schematic diagram of the partition and feeding adjustment plate structure of this utility model.

[0027] Figure 3 This is a schematic diagram of the structure at the location of the planetary gear of this utility model.

[0028] In the diagram: Fermentation tank 1, primary fermentation chamber 101, deep fermentation chamber 102, feeding port 103, discharging port 104, partition 2, first discharge hole 201, discharge adjustment plate 3, second discharge hole 301, convex plate 302, outer rotating shaft 4, first stirring blade 401, inner rotating shaft 5, second stirring blade 501, spiral blade 6, sun gear 7, planetary pinion 8, vertical cylinder 9, gear ring 901, geared motor 10, heat exchange jacket 11, heat exchange medium inlet pipe 111, heat exchange medium outlet pipe 112, connecting pipe 12, temperature sensor 13, controller 14, heat exchange medium transfer pump 15, adjusting vertical rod 16, fixing block 17, arc-shaped hole 171. Detailed Implementation

[0029] like Figure 1 In the present invention, a multi-stage solid-state fermentation device for amylase includes a fermentation tank 1, wherein a partition 2 is provided inside the fermentation tank 1, the partition 2 dividing the internal cavity of the fermentation tank 1 into an upper primary fermentation chamber 101 and a lower deep fermentation chamber 102.

[0030] The primary fermentation chamber 101 is provided with an outer rotating shaft 4, and an inner rotating shaft 5 is provided inside the outer rotating shaft 4. The lower end of the inner rotating shaft 5 passes downward through the partition 2 and extends into the deep fermentation chamber 102. A first stirring blade 401 is provided on the outer wall of the outer rotating shaft 4, and a second stirring blade 501 is provided on the outer wall of the inner rotating shaft 5 located in the deep fermentation chamber 102.

[0031] The inner rotating shaft 5 located inside the outer rotating shaft 4 is connected to the outer rotating shaft 4 by a planetary gear set. The top of the fermentation tank 1 is equipped with a reduction motor 10, and the drive shaft of the reduction motor 10 extends downward into the fermentation tank 1 and is connected to the upper shaft of the inner rotating shaft 5.

[0032] In a preferred embodiment, the planetary gear set includes a sun gear 7 disposed on the inner rotating shaft 5 and planetary pinions 8 disposed in multiple openings on the side wall of the outer rotating shaft 4, wherein the sun gear 7 meshes with the planetary pinions 8;

[0033] The fermenter 1 has a vertical cylinder 9 on its inner top surface. A gear ring 901 is provided on the inner wall of the lower end of the vertical cylinder 9. The gear ring 901 is located at the same horizontal height as the sun gear 7 and the planetary pinion 8. Multiple planetary pinions 8 mesh with the gear ring 901 at the same time.

[0034] In a preferred embodiment, the primary fermentation chamber 101 is provided with a feeding port 103 on its side wall, and the deep fermentation chamber 102 is provided with a discharge port 104 at its bottom.

[0035] The lower end of the inner rotating shaft 5 extends into the discharge port 104, and the inner rotating shaft 5 located in the discharge port 104 is provided with spiral blades 6.

[0036] In a preferred embodiment, a feeding adjustment plate 3 is provided below the partition 2. The partition 2 is provided with a plurality of radially arranged first feeding holes 201, and the feeding adjustment plate 3 is provided with a plurality of radially arranged second feeding holes 301. The shape and size of a single first feeding hole 201 and a single second feeding hole 301 are the same.

[0037] The partition 2 is fixedly installed inside the fermentation tank 1, and the feeding adjustment plate 3 is movably installed below the partition 2 and fits against the partition 2, and the feeding adjustment plate 3 can rotate around its own center.

[0038] In a preferred embodiment, a protrusion 302 is provided on one side of the feeding adjustment plate 3, and the protrusion 302 extends to the outside of the fermentation tank 1.

[0039] In a preferred embodiment, an adjusting vertical rod 16 is provided on the protrusion 302 outside the fermentation tank 1, and a fixing block 17 is provided on the outer wall of the fermentation tank 1 above the protrusion 302. The fixing block 17 is provided with an arc-shaped hole 171, and the adjusting vertical rod 16 is set to pass upward through the arc-shaped hole 171.

[0040] In a preferred embodiment, heat exchange jackets 11 are provided on the outer walls of both the primary fermentation chamber 101 and the deep fermentation chamber 102, and the two heat exchange jackets 11 are connected by a connecting pipe 12.

[0041] The heat exchange jacket 11 on the outer wall of the deep fermentation chamber 102 has a heat exchange medium inlet pipe 111 at the bottom edge of the side wall, and the heat exchange jacket 11 on the outer wall of the primary fermentation chamber 101 has a heat exchange medium outlet pipe 112 at the top edge of the side wall.

[0042] In a preferred embodiment, a heat exchange medium transfer pump 15 is provided on the heat exchange medium inlet pipe 111;

[0043] Temperature sensors 13 are installed on the side walls of the primary fermentation chamber 101 and the deep fermentation chamber 102. The two temperature sensors 13 are connected to the controller 14, which is connected to the heat exchange medium transfer pump 15 and the geared motor 10.

[0044] The present invention discloses a multi-stage solid-state fermentation device for amylase, which performs amylase fermentation operations as follows:

[0045] 1) Preparation stage

[0046] Choose a suitable solid substrate, such as starch-rich raw materials like grains or soybean meal, and perform necessary pretreatment, such as crushing and sterilization. Then, inoculate the inoculum containing the target microorganism (e.g., amylase-producing bacteria or fungi) evenly into the pretreated solid substrate. The inoculation amount is usually determined based on experimental conditions and expected yield.

[0047] 2) Initial fermentation stage

[0048] Turn on the geared motor 10 to start rotating the outer shaft 4 and the inner shaft 5, and use the first stirring blade 401 to fully mix the material in the primary fermentation chamber 101. At the same time, the internal temperature of the fermenter is regulated by cooling water or other heat exchange medium in the heat exchange jacket 11 to ensure that it is maintained within the range most suitable for the growth and reproduction of microorganisms, generally around 30°C.

[0049] Under suitable temperature and oxygen supply conditions, the inoculated microorganisms begin to proliferate rapidly and secrete amylase. As the fermentation process progresses, the microorganisms gradually consume the nutrients in the substrate and produce a large number of metabolites, including amylase.

[0050] During the above process, the geared motor 10 drives the first stirring blade 401 to rotate at high speed to increase the fermentation speed.

[0051] 3) Material transfer

[0052] When the initial fermentation reaches a certain time point, such as 24 hours, the operator can adjust the position of the feeding adjustment plate 3 by rotating the adjusting rod 16, so that some of the material that has completed the initial fermentation flows into the deep fermentation chamber 102 through the first feeding hole 201 on the partition plate 2 aligned with the second feeding hole 301 on the feeding adjustment plate 3. This process can be carried out in batches to ensure that the material transferred each time can be fully fermented.

[0053] 4) Deep fermentation stage

[0054] The material entering the deep fermentation chamber 102 continues to be acted upon by the second stirring blade 501, maintaining good ventilation and material mixing. Simultaneously, the fermentation temperature continues to be precisely controlled through the heat exchange jacket 11. The temperature setting may need to be adjusted appropriately according to the needs of the later stages of fermentation. During the deep fermentation stage, microorganisms continue to decompose the remaining starch, increasing the concentration of amylase. This stage is crucial for improving the purity and activity of the final product.

[0055] During the above process, the second stirring blade 501 is in a low-speed rotation state through the transmission of planetary gears, thereby ensuring the fermentation quality.

[0056] 5) Harvesting and Processing

[0057] After a total fermentation time of approximately 72 hours, the stirring system is stopped, and the spiral blades 6 are used to help discharge the material (the geared motor 10 in the fermentation process rotates in the opposite direction to the geared motor 10 in the discharge process). At this point, the material in the fermentation tank is rich in highly active amylase. The collected material needs to undergo physical separation steps such as filtration and centrifugation to remove solid residues. Then, it is prepared into finished amylase powder or liquid form through methods such as concentration and drying. Finally, the finished product is subjected to quality testing, including enzyme activity determination and purity analysis, to ensure that the product meets the standard requirements.

Claims

1. A multi-stage solid-state fermentation device for amylase, comprising a fermenter (1), characterized in that: The fermentation tank (1) is provided with a partition (2), which divides the internal cavity of the fermentation tank (1) into an upper primary fermentation chamber (101) and a lower deep fermentation chamber (102). The primary fermentation chamber (101) is provided with an outer rotating shaft (4), and an inner rotating shaft (5) is provided inside the outer rotating shaft (4). The lower end of the inner rotating shaft (5) passes downward through the partition (2) and extends into the deep fermentation chamber (102). A first stirring blade (401) is provided on the outer wall of the outer rotating shaft (4), and a second stirring blade (501) is provided on the outer wall of the inner rotating shaft (5) located in the deep fermentation chamber (102). The inner rotating shaft (5) located inside the outer rotating shaft (4) is connected to the outer rotating shaft (4) by a planetary gear set. A reduction motor (10) is provided on the top of the fermentation tank (1). The drive shaft of the reduction motor (10) extends downward into the fermentation tank (1) and is connected to the upper shaft of the inner rotating shaft (5). The planetary gear set includes a sun gear (7) mounted on the inner rotating shaft (5) and planetary pinions (8) mounted in multiple openings on the side wall of the outer rotating shaft (4). The sun gear (7) meshes with the planetary pinions (8). The fermenter (1) has a vertical cylinder (9) on its inner top surface. A gear ring (901) is provided on the inner wall of the lower end of the vertical cylinder (9). The gear ring (901) is located at the same horizontal height as the sun gear (7) and the planetary pinion (8). Multiple planetary pinions (8) mesh with the gear ring (901) at the same time. Below the partition (2) is a feeding adjustment plate (3). The partition (2) has a plurality of first feeding holes (201) arranged radially. The feeding adjustment plate (3) has a plurality of second feeding holes (301) arranged radially. The shape and size of a single first feeding hole (201) and a single second feeding hole (301) are the same. The partition (2) is fixedly installed inside the fermentation tank (1), and the feeding adjustment plate (3) is movably installed below the partition (2) and in contact with the partition (2), and the feeding adjustment plate (3) can rotate around its own center. The outer walls of the primary fermentation chamber (101) and the deep fermentation chamber (102) are provided with heat exchange jackets (11), and the two heat exchange jackets (11) are connected by a connecting pipe (12); The heat exchange jacket (11) on the outer wall of the deep fermentation chamber (102) is provided with a heat exchange medium inlet pipe (111) at the bottom edge of the side wall, and the heat exchange jacket (11) on the outer wall of the primary fermentation chamber (101) is provided with a heat exchange medium outlet pipe (112) at the top edge of the side wall. A heat exchange medium transfer pump (15) is provided on the heat exchange medium inlet pipe (111); Temperature sensors (13) are installed on the side walls of the primary fermentation chamber (101) and the deep fermentation chamber (102). The two temperature sensors (13) are connected to the controller (14), which is connected to the heat exchange medium transfer pump (15) and the geared motor (10).

2. The amylase multi-stage solid-state fermentation device according to claim 1, characterized in that: The primary fermentation chamber (101) is provided with a feeding port (103) on its side wall, and the deep fermentation chamber (102) is provided with a discharge port (104) at its bottom. The lower end of the inner rotating shaft (5) extends into the discharge port (104), and the inner rotating shaft (5) portion located in the discharge port (104) is provided with a spiral blade (6).

3. The amylase multi-stage solid-state fermentation device according to claim 1, characterized in that: The feeding adjustment plate (3) has a protrusion (302) on one side, which extends to the outside of the fermentation tank (1).

4. The amylase multi-stage solid-state fermentation device according to claim 3, characterized in that: An adjusting vertical rod (16) is provided on the protrusion (302) outside the fermentation tank (1). A fixing block (17) is provided on the outer wall of the fermentation tank (1) above the protrusion (302). An arc-shaped hole (171) is provided on the fixing block (17). The adjusting vertical rod (16) is set to pass through the arc-shaped hole (171) upward.

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