Auxiliary device for separating and extracting PBMC
By designing a limiting device consisting of components such as a fixing ring, connecting strip, cap, and positioning column, the problem of sample leakage caused by the cap popping open during centrifugation of capped centrifuge tubes was solved, thus ensuring the smooth progress of the experiment and the safety of the samples.
Patent Information
- Application Number
- CN202423273237.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-30
- Publication Date
- 2026-01-09
- Estimated Expiration
- 2034-12-30
AI Technical Summary
Existing capped centrifuge tubes may open due to excessive pressure during centrifugation, causing sample leakage and affecting the smooth progress of the experiment.
An auxiliary device was designed, including components such as a fixing ring, connecting strip, pressure cap, positioning post and rubber ring. Through the cooperation of these components, the pressure cap is limited and sealed to prevent it from popping open during centrifugation.
This effectively prevented sample leakage during centrifugation, ensuring the smooth progress of the experiment and the integrity of the samples.
Smart Images
Figure CN223780253U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of PBMC separation and extraction technology, and in particular to an auxiliary device for separating and extracting PBMC. Background Technology
[0002] PBMC is an abbreviation for peripheral blood mononuclear cells. It is a mixed population of mononuclear cells in peripheral blood. These cells play a key role in many physiological processes such as the immune response in the human body. In order to remove multinucleated cells and red blood cells, so as to facilitate the study of mononuclear cells, researchers need to separate and extract PBMCs. In order to ensure the integrity and stability of the sample, centrifuge tubes are needed in the separation and extraction process. They are very important auxiliary tools for separating and extracting PBMCs. To save time opening the cap, the capped centrifuge tubes have been favored by researchers.
[0003] In practical use of capped centrifuge tubes, researchers only need to apply appropriate external force to pull off the cap to open the centrifuge tube. Since the cap is sealed to the inner wall of the tube opening by friction, when the centrifuge tube is placed in the centrifuge for extraction, the centrifugal force will affect the liquid and air inside the tube. If the centrifuge tube contains a large amount of liquid or the sample contains volatile components, the liquid may expand or produce gas under the strong centrifugal force, which will increase the pressure inside the tube. Once the pressure inside the tube exceeds the limit that the cap can withstand, the centrifuge tube may open by itself, causing sample leakage. To address this problem, we propose an auxiliary device for the separation and extraction of PBMCs. Utility Model Content
[0004] The purpose of this invention is to address the shortcomings of existing technologies by proposing an auxiliary device for separating and extracting PBMCs.
[0005] To achieve the above objectives, the present invention adopts the following technical solution:
[0006] An auxiliary device for separating and extracting PBMCs includes a centrifuge tube. A fixing ring is connected to the top of the centrifuge tube, and the side of the fixing ring is connected to a cap via a connecting strip. An insert tube is connected to the side of the cap. A rectangular groove is formed inside the cap, and a connecting block is inserted into the inner wall of the rectangular groove. The side of the connecting block is connected to the inner wall of the rectangular groove via a connecting spring. An insertion block is connected to the bottom of the connecting block. A mating groove that matches the insertion block is formed on the side of the fixing ring.
[0007] Preferably, the top of the connecting block is connected to a pusher strip, and the outer wall of the pusher strip is uniformly distributed with anti-slip particles.
[0008] Preferably, a connecting rod is inserted into the inner wall of the rectangular groove, and a slot adapted to the connecting rod is opened inside the connecting block.
[0009] Preferably, there are two sets of connecting rods, and the two sets of connecting rods are symmetrically distributed about the central axis of the rectangular groove.
[0010] Preferably, the centrifuge tube is connected to a positioning post on its side, and the connecting strip has a connecting groove inside that is adapted to the positioning post.
[0011] Preferably, the outer wall of the positioning post is bonded with a rubber ring, and the cross-section of the rubber ring is set as an arc-shaped structure.
[0012] Preferably, the top end of the fixing ring is connected to a connecting ring, and the side of the pressure cap is provided with an annular groove that is adapted to the connecting ring.
[0013] Compared with the prior art, the beneficial effects of this utility model are as follows:
[0014] 1. This device is equipped with connecting blocks and insertion blocks. After the cap is covered, the connecting blocks and insertion blocks work together to limit the cap again, which can effectively prevent the cap from being pushed open during centrifugation and prevent sample leakage, thus ensuring the smooth progress of the experiment.
[0015] 2. The device is equipped with a positioning column and a rubber ring. When the sample is poured in or out, the positioning column and the rubber ring work together to limit the opening of the cap, which effectively prevents the cap from blocking the pouring operation, thus ensuring the reliability of the device during use. Attached Figure Description
[0016] Figure 1 This is a three-dimensional structural schematic diagram of an auxiliary device for separating and extracting PBMCs according to the present invention.
[0017] Figure 2 for Figure 1 A three-dimensional cross-sectional view of the connecting strip and the gland structure in the middle;
[0018] Figure 3 for Figure 1 A three-dimensional cross-sectional view of the connecting ring and fixing ring structure in the diagram;
[0019] Figure 4 For the present utility model Figure 3 Enlarged schematic diagram of the structure at point A in the middle;
[0020] Figure 5 for Figure 1 A three-dimensional cross-sectional diagram of the positioning post and rubber ring structure.
[0021] In the diagram: 1. Centrifuge tube; 2. Connecting strip; 3. Pressure cap; 4. Rectangular groove; 5. Connecting block; 6. Insertion block; 7. Connecting spring; 8. Connecting rod; 9. Push bar; 10. Connecting ring; 11. Positioning post; 12. Rubber ring; 13. Embedded tube; 14. Fixing ring. Detailed Implementation
[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.
[0023] Reference Figure 1-5 An auxiliary device for separating and extracting PBMCs includes a centrifuge tube 1. A fixing ring 14 is connected to the top of the centrifuge tube 1, and the side of the fixing ring 14 is connected to a pressure cap 3 via a connecting strip 2. The connecting strip 2 is made of plastic and has a certain elasticity and toughness, so that the connecting strip 2 can be bent so that the pressure cap 3 can be opened and closed smoothly. An inserting tube 13 is connected to the side of the pressure cap 3. A rectangular groove 4 is opened inside the pressure cap 3, and a connecting block 5 is inserted into the inner wall of the rectangular groove 4. The side of the connecting block 5 is connected to the inner wall of the rectangular groove 4 via a connecting spring 7. An insertion block 6 is connected to the bottom of the connecting block 5. A mating groove that matches the insertion block 6 is opened on the side of the fixing ring 14.
[0024] Furthermore, refer to Figure 2 and Figure 4 It can be seen that the top of the connecting block 5 is connected to the push bar 9, and the outer wall of the push bar 9 is evenly distributed with anti-slip particles. Through the cooperation of the push bar 9 and the anti-slip particles, it is convenient for the staff to quickly and manually push the connecting block 5 to move laterally, so as to effectively improve the convenience of using this device.
[0025] Furthermore, refer to Figure 4 It can be seen that the inner wall of the rectangular groove 4 is fitted with a connecting rod 8, and the inside of the connecting block 5 is provided with a slot that matches the connecting rod 8. During the process of the push bar 9 driving the connecting block 5 to move laterally, the connecting block 5 can be subjected to force and slide along the outer surface of the connecting rod 8, thereby achieving lateral guidance of the connecting block 5 and effectively preventing the connecting spring 7 from becoming skewed.
[0026] Furthermore, refer to Figure 4 It can be seen that there are two sets of connecting rods 8, and the two sets of connecting rods 8 are symmetrically distributed about the central axis of the rectangular groove 4. The setting of the two sets of connecting rods 8 can further ensure the stable translation of the connecting block 5.
[0027] Furthermore, refer to Figure 5It can be seen that the centrifuge tube 1 is connected to the side of the positioning post 11, and the connecting strip 2 has a connecting groove that matches the positioning post 11. After the cap 3 is opened, the operator can insert the connecting strip 2 into the positioning post 11. Through the friction between the connecting groove and the positioning post 11, the connecting strip 2 can be initially limited, which can effectively prevent the cap 3 from being easily rotated and affecting the operator's liquid pouring.
[0028] Furthermore, refer to Figure 5 It can be seen that the outer wall of the positioning post 11 is glued with a rubber ring 12, and the cross-section of the rubber ring 12 is set as an arc structure. When the connecting strip 2 is inserted into the outer wall of the positioning post 11, the connecting groove inside the connecting strip 2 can pass through the rubber ring 12 set by the arc structure. Through the setting of the rubber ring 12, the inserted rubber ring 12 can be limited again, thereby effectively improving the limiting effect on the pressure cap 3.
[0029] Furthermore, refer to Figure 1 and Figure 3 It can be seen that the top of the fixing ring 14 is connected to the connecting ring 10, and the side of the pressure cap 3 is provided with an annular groove that matches the connecting ring 10. When the pressure cap 3 is put on, the annular groove connected to the side of the pressure cap 3 can be inserted into the interior of the connecting ring 10. The connecting ring 10 and the annular groove provided by the annular structure can effectively ensure the sealing of the pressure cap 3 during the covering process, so as to further prevent leakage.
[0030] Working principle: When using this utility model, the operator can first manually push the push bar 9 to simultaneously force the connecting block 5 and the insertion block 6, thereby causing the insertion block 6 to be pulled out from the mating groove on the side of the fixing ring 14. At this time, the operator can lift the pressure cap 3, thereby pulling out the embedded tube 13 connected to its side from the top opening of the centrifuge tube 1. When the pressure cap 3 is rotated to fit against the outer wall of the centrifuge tube 1, the operator can insert the connecting groove inside the connecting bar 2 into the outer wall of the positioning post 11, and limit the positioning post 11 after it is inserted by the rubber ring 12. At this time, the operator... The PBMC sample can be poured into the centrifuge tube 1. After pouring, the operator can first pull the connecting strip 2 to pull it out of the positioning column 11, and then cover the top of the centrifuge tube 1 with the cap 3. During the covering process, the trapezoidal insertion block 6 can be automatically locked into the docking groove inside the fixing ring 14 by the elastic force of the connecting spring 7, so as to limit the cap 3 and effectively prevent the cap 3 from popping open during centrifugation. Then the operator can insert the centrifuge tube 1 into the centrifuge equipment for separation and extraction. The above is the entire working principle of this utility model.
[0031] In this utility model, the installation, connection or setting methods of all the components mentioned above are common mechanical methods, and the specific structure, model and coefficient index of all the components are their own technologies. As long as they can achieve their beneficial effects, they can be implemented, so they will not be described in detail.
[0032] The above embodiments are preferred embodiments of the present utility model, but the embodiments of the present utility model are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present utility model shall be considered equivalent substitutions and shall be included within the protection scope of the present utility model.
[0033] In this utility model, unless otherwise stated, directional terms such as "up, down, left, right, front, back, inside, outside, and vertical and horizontal" in the terminology only represent the orientation of the term in its conventional use or are common names understood by those skilled in the art, and should not be regarded as limitations on the term. At the same time, numerals such as "first," "second," and "third" do not represent specific quantities or orders, but are only used to distinguish names. Moreover, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a series of elements includes not only those elements, but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.
Claims
1. An auxiliary device for separating and extracting PBMCs, comprising centrifuge tubes (1), characterized in that, The centrifuge tube (1) is connected to a fixing ring (14) at the top end, and the side of the fixing ring (14) is connected to the pressure cap (3) via a connecting strip (2). The side of the pressure cap (3) is connected to an inserting tube (13). A rectangular groove (4) is opened inside the pressure cap (3), and a connecting block (5) is inserted into the inner wall of the rectangular groove (4). The side of the connecting block (5) is connected to the inner wall of the rectangular groove (4) via a connecting spring (7). An inserting block (6) is connected to the bottom end of the connecting block (5). A mating groove that matches the inserting block (6) is opened on the side of the fixing ring (14).
2. The auxiliary device for separating and extracting PBMCs according to claim 1, characterized in that, The top of the connecting block (5) is connected to a pusher (9), and the outer wall of the pusher (9) is evenly distributed with anti-slip particles.
3. The auxiliary device for separating and extracting PBMCs according to claim 1, characterized in that, The inner wall of the rectangular groove (4) is provided with a connecting rod (8), and the inside of the connecting block (5) is provided with a slot that matches the connecting rod (8).
4. The auxiliary device for separating and extracting PBMCs according to claim 3, characterized in that, The connecting rod (8) is provided in two sets, and the two sets of connecting rods (8) are symmetrically distributed about the central axis of the rectangular groove (4).
5. The auxiliary device for separating and extracting PBMCs according to claim 1, characterized in that, The centrifuge tube (1) is connected to a positioning post (11) on its side, and the connecting strip (2) has a connecting groove inside that is compatible with the positioning post (11).
6. The auxiliary device for separating and extracting PBMCs according to claim 5, characterized in that, The outer wall of the positioning post (11) is glued with a rubber ring (12), and the cross-section of the rubber ring (12) is set as an arc structure.
7. The auxiliary device for separating and extracting PBMCs according to claim 1, characterized in that, The top of the fixing ring (14) is connected to the connecting ring (10), and the side of the pressure cap (3) is provided with an annular groove that is adapted to the connecting ring (10).