PCR (Polymerase Chain Reaction) sequencing product purification equipment

Abstract

The utility model provides PCR sequencing product purification equipment which comprises an equipment shell, a sliding cylinder is arranged on the inner side wall of the equipment shell, the output end of the sliding cylinder is in sliding connection with a sliding rail block, the bottom of the sliding rail block is in sliding connection with a sterilization assembly, the bottom of the sliding rail block is in sliding connection with a clamping assembly, and the side wall of the equipment shell is in sliding connection with a PCR plate storage drawer. According to the utility model, the PCR plate can be disinfected and sterilized in all directions through the disinfecting and sterilizing assembly, and the clamping assembly can be used for grabbing the PCR plate without contacting with the PCR plate, so that the PCR plate is moved to the upper part of the oscillation assembly, and the PCR plate can be stored in the equipment shell through the oscillation assembly. The temperature of liquid in the PCR plate for sequencing is controlled, so that the liquid is adjusted to the required reaction temperature, and the liquid is fully mixed under vibration, so that not only is the accuracy of sequencing improved, but also the risk of being polluted is remarkably reduced.

Description

TECHNICAL FIELD

[0001] The utility model relates to PCR sequencing technical field especially relates to a PCR sequencing product purification equipment. BACKGROUND

[0002] PCR is a kind of technology widely used in molecular biology field, it can amplify specific DNA fragment in short time, and provides important basis for subsequent analysis and research, PCR sequencing is the sequencing analysis of DNA using PCR technology, and is one of important means in genomics research, and the experiment application of PCR sequencing needs to oscillate PCR plate and make it warm or cool to ensure that reactants are fully mixed and reach the required reaction temperature.

[0003] In the related art, the utility model discloses a PCR reaction liquid transfer device, including outer box, the side of outer box is fixedly connected with side plate, the bottom of side plate is hinged with box door, the bottom of outer box is fixedly connected with a plurality of baffle, the side of baffle is slidably connected with hole position plate, the top of outer box is slidably connected with connecting rod, the bottom of connecting rod is fixedly connected with fixed block, the bottom of fixed block is fixedly connected with cover plate.

[0004] According to the above prior art, the inventor combines related technologies, and finds that the utility model only solves the problem of liquid splashing during movement in actual application, but in the sequencing process of PCR, accurate temperature control to ensure that reactants are fully mixed is crucial, because the activity of DNA polymerase is very sensitive to temperature, and the lack of sterilization of PCR plate increases the possibility of contamination, therefore, it is very necessary to provide a device that can control temperature, fully oscillate and mix, and can be sterilized. UTILITY MODEL CONTENT

[0005] In order to solve the problems of not being able to control temperature, fully oscillate and mix, and not being able to sterilize in the prior art, the utility model provides a PCR sequencing product purification equipment;

[0006] The utility model provides a kind of PCR sequencing product purification equipment using following technical scheme:

[0007] A kind of PCR sequencing product purification equipment, including equipment shell, the inner side wall of the equipment shell is provided with sliding cylinder, the output end of the sliding cylinder is slidably connected with slide rail block, the slide rail block bottom is slidably connected with sterilization and disinfection component, the slide rail block bottom is slidably connected with clamping component, the side wall of the equipment shell is slidably connected with PCR plate storage drawer, the side wall of the PCR plate storage drawer is provided with PCR plate, the inside of the equipment shell is provided with oscillation component.

[0008] Further, the sterilization assembly comprises a sterilization electric cylinder, a sterilization connecting block, a sterilization motor, a sterilization plate block and an ultraviolet lamp, the bottom of the sliding rail block is connected with the sterilization electric cylinder through a sliding rail, the output end of the sterilization electric cylinder is detachably connected with the sterilization connecting block, the inside of the sterilization connecting block is connected with the sterilization motor through bolts, the output end of the sterilization motor is detachably fixedly connected with the sterilization plate block, the sterilization plate block is rotationally connected with the sterilization connecting block, and the side wall of the sterilization plate block is provided with the ultraviolet lamp.

[0009] Further, the clamping assembly comprises a clamping electric cylinder, a clamping driving block, a clamping motor, a clamping gear and a clamping gear rod, the bottom of the sliding rail block is connected with the clamping electric cylinder through a sliding rail, the output end of the clamping electric cylinder is detachably connected with the clamping driving block, the inside of the clamping driving block is connected with the clamping motor through bolts, the output end of the clamping motor is connected with the clamping gear through a connecting shaft, and the opposite two side walls of the clamping driving block are respectively slidably connected with the clamping gear rods.

[0010] Further, the oscillation assembly comprises a vibration platform, a vibration motor, oscillation springs, oscillation support blocks, oscillation support columns, a support base, an oscillation plate groove and a temperature control unit, the inside of the equipment shell is fixedly connected with the support base through bolts, the four corners of the support base are respectively provided with the oscillation support columns, the oscillation support blocks are welded to the four oscillation support columns, the inside of the four oscillation support blocks is detachably connected with one end of the oscillation springs, the other end of the oscillation springs is detachably connected with the vibration platform, the bottom of the vibration platform is connected with the vibration motor through bolts, the top of the vibration platform is provided with the oscillation plate groove, and the inside of the vibration platform is provided with the temperature control unit.

[0011] Further, the temperature control unit comprises a fan, a fan frame, a cold and hot circulating pump, a circulating pipe and a conduction block, the inside of the vibration platform is connected with the fan frame through bolts, the inside of the fan frame is rotationally connected with the fan, the top of the fan is provided with the circulating pipe, the inside of the vibration platform is connected with the cold and hot circulating pump through bolts, the water inlet end and the water outlet end of the cold and hot circulating pump are connected with the circulating pipe, and the side wall of the vibration platform is detachably connected with the conduction block.

[0012] Further, the ultraviolet lamp can cooperate with the PCR plate, the ultraviolet lamps are arranged in an array in the transverse direction and the longitudinal direction of the sterilization plate block, the number of the ultraviolet lamps is N, and N is greater than or equal to 2.

[0013] Further, the opposite side walls of the PCR plate are respectively provided with clamping grooves, and the two clamping gear rods are detachably connected with clamping protrusions relative to the two side walls.

[0014] In summary, the utility model discloses the beneficial effect is:

[0015] The utility model discloses through killing assembly, PCR board can get all -round disinfection and sterilization to avoid the possible pollution, also improved the accuracy and reliability of PCR sequencing process, through the clamping assembly, make can be in the case not contacting PCR board complete snatch, thereby make its displacement to the above of oscillation subassembly, further through oscillation subassembly, the liquid in the PCR board of sequencing carries out temperature control and makes it adjust to the reaction temperature required, and fully mix under the vibration, the utility model discloses, not only improve the accurate degree of sequencing, also significantly reduce the risk of being contaminated. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 It is whole structure schematic view of the utility model;

[0017] Figure 2 It is whole structure front view schematic view of the utility model;

[0018] Figure 3 It is whole structure schematic view of the utility model Figure 2 It is A-A section schematic view of the utility model;

[0019] Figure 4 It is killing assembly and clamping assembly three -dimensional schematic view of the utility model;

[0020] Figure 5 It is internal conclusion oscillation subassembly three -dimensional schematic view of the utility model;

[0021] Figure 6 It is killing assembly and clamping assembly section schematic view of the utility model;

[0022] Figure 7 It is B-B section schematic view of the utility model Figure 6 ;

[0023] Figure 8 It is A part enlarged schematic view of the utility model Figure 3 ;

[0024] As shown in the figure: 1-equipment shell, 2-PCR board storage drawer, 3-vibration platform, 4-sliding cylinder, 5-killing electric cylinder, 6-clamping electric cylinder, 7-oscillation plate groove, 8-vibration motor, 9-cold and hot circulation pump, 10-killing plate, 11-clamping drive block, 12-PCR board, 13-clamping groove, 14-circulation pipe, 15-ultraviolet lamp, 16-killing motor, 17-clamping gear rod, 18-clamping gear, 19-clamping motor, 20-fan, 21-fan frame, 22-oscillation spring, 23-oscillation support block, 24-oscillation support column, 25-sliding rail block, 26-killing connecting block, 27-conducting block, 28-support base. DETAILED DESCRIPTION

[0025] The accompanying drawings are included to provide a further understanding of the present application and are incorporated in and constitute a part of this specification. Figures 1-8 The present application is further described in detail:

[0026] The present application discloses a PCR sequencing product purification device, which comprises a device shell 1, a sliding cylinder 4 is arranged on the inner side wall of the device shell 1, a sliding rail block 25 is slidably connected to the output end of the sliding cylinder 4, a sterilization and disinfection assembly is slidably connected to the bottom of the sliding rail block 25, a clamping assembly is slidably connected to the bottom of the sliding rail block 25, a PCR plate storage drawer 2 is slidably connected to the side wall of the device shell 1, a PCR plate 12 is arranged on the side wall of the PCR plate storage drawer 2, and a shaking assembly is arranged in the device shell 1. Figure 1 , Figure 2 , Figure 3 , Figure 4 In this embodiment, the sterilization and disinfection assembly is increased, so that the PCR plate 12 can be sterilized and disinfected in all directions to avoid the possibility of pollution, and the accuracy and reliability of the PCR sequencing process are improved.

[0027] As shown in Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 6 The sterilization and disinfection assembly comprises a sterilization and disinfection electric cylinder 5, a sterilization and disinfection connecting block 26, a sterilization and disinfection motor 16, a sterilization and disinfection plate block 10 and an ultraviolet lamp 15, the bottom of the sliding rail block 25 is connected with the sterilization and disinfection electric cylinder 5 through a sliding rail, the output end of the sterilization and disinfection electric cylinder 5 is detachably connected with the sterilization and disinfection connecting block 26, the sterilization and disinfection motor 16 is connected inside the sterilization and disinfection connecting block 26 through bolts, the output end of the sterilization and disinfection motor 16 is detachably fixedly connected with the sterilization and disinfection plate block 10, the sterilization and disinfection plate block 10 is rotationally connected with the sterilization and disinfection connecting block 26, and the side wall of the sterilization and disinfection plate block 10 is provided with the ultraviolet lamp 15.

[0028] In this embodiment, the sterilization and disinfection motor 16 is increased, so that the output end of the sterilization and disinfection motor 16 drives the sterilization and disinfection plate block 10, the ultraviolet lamp 15 faces the top of the PCR plate 12, the sterilization and disinfection electric cylinder 5 is started, the ultraviolet lamp 15 is tightly attached to the top of the PCR plate 12, and sufficient sterilization and disinfection are performed. Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 6As shown, the clamping assembly includes clamping cylinder 6, clamping drive block 11, clamping motor 19, clamping gear 18, clamping gear rod 17, the bottom of the slide rail block 25 is connected with clamping cylinder 6 through slide rail, the output end of clamping cylinder 6 is detachably connected with clamping drive block 11, clamping motor 19 is connected inside clamping drive block 11 through bolt, the output end of clamping motor 19 is connected with clamping gear 18 through connecting shaft, the opposite two side walls of clamping drive block 11 are respectively connected with clamping gear rod 17, two clamping gear rods 17 are meshed with clamping gear 18 on both sides of clamping gear 18, in this embodiment, by increasing clamping cylinder 6, the output end of clamping cylinder 6 drives clamping drive block 11, so that the clamping gear rod 17 inside clamping drive block 11 is displaced to both sides of PCR plate 12, then by starting clamping motor 19, the output end of clamping motor 19 drives clamping gear 18 to rotate, so that the two clamping gear rods 17 meshed with it shrink inward, and then the clamping lugs on the two clamping gear rods 17 are clamped with the clamping grooves 13 on both sides of PCR plate 12, and the PCR plate 12 is stably displaced into the oscillation plate groove 7, realizing the displacement of grabbing without contacting PCR plate 12, improving the accuracy of sequencing, and reducing the possibility of PCR plate 12 being contaminated.

[0029] As shown in Figure 1 , Figure 3 , Figure 5 , Figure 8 , the oscillation assembly includes vibration platform 3, vibration motor 8, oscillation spring 22, oscillation support block 23, oscillation support column 24, support base 28, oscillation plate groove 7, temperature control unit, the inner side wall of equipment shell 1 is fixedly connected with support base 28 through bolt, the four corners of support base 28 are respectively provided with oscillation support column 24, four oscillation support columns 24 are welded with oscillation support block 23, the adjacent three faces of the inner side of four oscillation support blocks 23 are detachably connected with one end of oscillation spring 22, the other end of a plurality of oscillation springs 22 is detachably connected with vibration platform 3, vibration motor 8 is connected at the bottom of vibration platform 3 through bolt, oscillation plate groove 7 is opened at the top of vibration platform 3, temperature control unit is arranged inside vibration platform 3, in this embodiment, by increasing vibration motor 8, PCR plate 12 is fully oscillated under vibration, so as to complete full mixing and improve accuracy.

[0030] As shown in Figure 1 , Figure 3 , Figure 5 , Figure 8As shown, the temperature control unit includes a fan 20, a fan frame 21, a cold and hot circulating pump 9, a circulating pipe 14, a conduction block 27, the inner side wall of the vibration platform 3 is connected with the fan frame 21 through bolts, the fan frame 21 is rotatably connected with the fan 20 inside, the circulating pipe 14 is arranged at the top of the fan 20, the cold and hot circulating pump 9 is connected with the circulating pipe 14 through bolts at the inner side wall of the vibration platform 3, the water inlet end and the water outlet end of the cold and hot circulating pump 9 are connected with the circulating pipe 14, the conduction block 27 is detachably connected with the side wall of the vibration platform 3, and the conduction block 27 is tightly attached to the oscillation plate groove 7; in the embodiment, the cold and hot circulating pump 9 is added, so that the temperature can be quickly conducted to the PCR plate 12 through the conduction block 27, thereby accurately controlling the temperature and ensuring that the reactants are fully mixed.

[0031] As shown in Figure 4 , Figure 6 , the ultraviolet lamp 15 can cooperate with the PCR plate 12, the ultraviolet lamp 15 is arranged in an array along the transverse direction and the longitudinal direction of the disinfection plate block 10, the number of the ultraviolet lamp 15 is N, and N≥2; in the embodiment, the number of the ultraviolet lamp 15 is increased, so that the array of the ultraviolet lamp 15 can cover the entire area of the PCR plate 12, and ensure that each sample is uniformly irradiated by ultraviolet rays.

[0032] As shown in Figure 4 , Figure 6 , the PCR plate 12 is provided with a clamping groove 13 on the opposite side wall, and the two clamping toothed rods 17 are detachably connected with clamping protrusions on the two side walls; in the embodiment, the clamping protrusions are matched with the clamping grooves 13, so that the PCR plate 12 can be stably displaced to the required position.

[0033] The implementation principle of the embodiment of the utility model is as follows:

[0034] In use, first, the operator places the PCR plate 12 into the PCR plate storage drawer 2, then slides the PCR plate storage drawer 2 into the inside of the equipment shell 1, and then starts the disinfection motor 16, so that the output end of the disinfection motor 16 drives the disinfection plate block 10, so that the ultraviolet lamp 15 is opposite to the top of the PCR plate 12, and then the disinfection cylinder 5 is started, so that the ultraviolet lamp 15 is tightly attached to the top of the PCR plate 12, and sufficient disinfection is performed;

[0035] Secondly, after sterilization is completed, the slide rail block 25 is driven to slide by the slide cylinder 4, so that the clamping electric cylinder 6 is opposite to the PCR plate 12, then the clamping electric cylinder 6 is started, so that the output end of the clamping electric cylinder 6 drives the clamping driving block 11, then the clamping gear rods 17 inside the clamping driving block 11 are displaced to the two sides of the PCR plate 12, then the clamping motor 19 is started, so that the output end of the clamping motor 19 drives the clamping gear 18 to rotate, so that the two clamping gear rods 17 engaged with the clamping gear 18 are inwards retracted, then the clamping protrusions on the two clamping gear rods 17 are clamped with the clamping grooves 13 on the two sides of the PCR plate 12, and the PCR plate 12 is stably displaced into the oscillation plate groove 7;

[0036] Finally, the temperature is increased or decreased by the cold and hot cycle pump 9 and the fan 20, so that the temperature is adjusted to the required temperature of the reaction, then the vibration motor 8 is started, so that the PCR plate 12 is fully vibrated, then the full mixing is completed.

[0037] The basic principle and main features of the utility model and the advantages of the utility model are shown and described. The various components mentioned in the utility model are common technologies in the prior art, which should be understood by the skilled in the art, and the utility model is not limited by the above-mentioned embodiments, the above-mentioned embodiments and the description in the specification are only to illustrate the principle of the utility model, various changes and improvements of the utility model can be made without departing from the spirit and scope of the utility model, and these changes and improvements all fall within the scope of the utility model claimed. The protection scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A PCR sequencing product purification apparatus comprising an apparatus housing (1), characterised in that, The inner side wall of the equipment shell (1) is provided with a sliding cylinder (4), the output end of the sliding cylinder (4) is slidably connected with a sliding rail block (25), the bottom of the sliding rail block (25) is slidably connected with a disinfection and killing assembly, the bottom of the sliding rail block (25) is slidably connected with a clamping assembly, the side wall of the equipment shell (1) is slidably connected with a PCR plate storage drawer (2), the side wall of the PCR plate storage drawer (2) is provided with a PCR plate (12), and the inside of the equipment shell (1) is provided with a shaking assembly.

2. A PCR sequencing product purification apparatus according to claim 1, characterised in that, The disinfection and killing assembly comprises a disinfection and killing electric cylinder (5), a disinfection and killing connecting block (26), a disinfection and killing motor (16), a disinfection and killing plate block (10) and an ultraviolet lamp (15), the bottom of the sliding rail block (25) is connected with the disinfection and killing electric cylinder (5) through a sliding rail, the output end of the disinfection and killing electric cylinder (5) is detachably connected with the disinfection and killing connecting block (26), the inside of the disinfection and killing connecting block (26) is boltedly connected with the disinfection and killing motor (16), the output end of the disinfection and killing motor (16) is detachably fixedly connected with the disinfection and killing plate block (10), the disinfection and killing plate block (10) is rotatably connected with the disinfection and killing connecting block (26), and the side wall of the disinfection and killing plate block (10) is provided with the ultraviolet lamp (15).

3. A PCR sequencing product purification apparatus according to claim 1, wherein, The clamping assembly comprises a clamping electric cylinder (6), a clamping driving block (11), a clamping motor (19), a clamping gear (18) and a clamping gear rod (17), the bottom of the sliding rail block (25) is connected with the clamping electric cylinder (6) through a sliding rail, the output end of the clamping electric cylinder (6) is detachably connected with the clamping driving block (11), the inside of the clamping driving block (11) is boltedly connected with the clamping motor (19), the output end of the clamping motor (19) is connected with the clamping gear (18) through a connecting shaft, and the opposite two side walls of the clamping driving block (11) are slidably connected with the clamping gear rods (17), respectively, and the two clamping gear rods (17) are meshedly connected with the clamping gear (18) on the two sides of the clamping gear (18).

4. A PCR sequencing product purification apparatus according to claim 1, wherein The shaking assembly comprises a shaking platform (3), a shaking motor (8), a shaking spring (22), a shaking support block (23), a shaking support column (24), a support base (28), a shaking plate groove (7) and a temperature control unit, the inner side wall of the equipment shell (1) is boltedly connected with the support base (28), the four corners of the support base (28) are respectively provided with the shaking support columns (24), the four shaking support columns (24) are welded with the shaking support blocks (23), the adjacent three faces of the four shaking support blocks (23) are detachably connected with one end of the shaking springs (22), the other end of the shaking springs (22) is detachably connected with the shaking platform (3), the bottom of the shaking platform (3) is boltedly connected with the shaking motor (8), the top of the shaking platform (3) is provided with the shaking plate groove (7), and the inside of the shaking platform (3) is provided with the temperature control unit.

5. A PCR sequencing product purification apparatus according to claim 4, characterised in that, The temperature control unit comprises a fan (20), a fan frame (21), a cold and hot circulating pump (9), a circulating pipe (14), and a conduction block (27), the inner side wall of the vibration platform (3) is connected with the fan frame (21) through bolts, the fan frame (21) is rotationally connected with the fan (20) inside, the top of the fan (20) is provided with the circulating pipe (14), the inner side wall of the vibration platform (3) is connected with the cold and hot circulating pump (9) through bolts, the water inlet end and the water outlet end of the cold and hot circulating pump (9) are connected with the circulating pipe (14), and the side wall of the vibration platform (3) is detachably connected with the conduction block (27); the conduction block (27) is tightly attached to the oscillation plate groove (7).

6. A PCR sequencing product purification apparatus according to claim 2, wherein, The ultraviolet lamp (15) can cooperate with the PCR plate (12), the ultraviolet lamp (15) is arranged in an array in the transverse direction and the longitudinal direction of the disinfecting plate block (10), the number of the ultraviolet lamp (15) is N, and N is greater than or equal to 2.

7. A PCR sequencing product purification apparatus according to claim 3, wherein The opposite side walls of the PCR plate (12) are respectively provided with clamping grooves (13), and the two clamping gear rods (17) are detachably connected with clamping protrusions.

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