Potassium bacteria culture device for rape planting soil

By designing a potassium-releasing bacteria cultivation device suitable for rapeseed planting soil, the problems of poor heat preservation and large tuber cultivation in existing devices were solved, achieving efficient screening of potassium-releasing bacteria and stability in rapeseed planting, thereby improving yield and quality.

CN223780265UActive Publication Date: 2026-01-09HUNAN CHEM VOCATIONAL TECH COLLEGE
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Patent Information

Application Number
CN202520102853.X
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2025-01-15
Publication Date
2026-01-09
Estimated Expiration
2035-01-15

AI Technical Summary

Technical Problem

Existing bacterial culture devices have poor heat preservation effects and cannot meet the cultivation needs of large tubers in rapeseed planting soil. Furthermore, it is difficult to control the amount of potassium fertilizer applied during rapeseed planting.

Method used

A potassium-solubilizing bacteria culture device for rapeseed planting soil was designed, comprising a heating chamber, a grinding mechanism, and a culture box with a magnetic suction structure. It can effectively keep warm and adapt to the cultivation needs of rapeseed planting soil. The grinding mechanism treats the clumped soil, the magnetic suction structure stably places the culture dish, and temperature and humidity sensors are equipped for monitoring.

Benefits of technology

The method achieved efficient cultivation of potassium-solubilizing bacteria in rapeseed planting soil, screened out suitable potassium-solubilizing bacteria, reduced the amount of potassium fertilizer applied, improved rapeseed yield and grain quality, and the cultivation process was stable and reliable.

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Abstract

The utility model discloses a culture device for potassium bacteria in rape planting soil, which comprises a culture box with an opening at the upper end, heating cavities arranged on the side walls and the bottom of the culture box, heating resistance wires arranged in the heating cavities, a plurality of bearing tables arranged on the inner wall of the culture box and used for placing culture dishes, supporting blocks placed on the bearing table are arranged on the two sides of the culture dish, a box cover is arranged at the upper end of the culture box, a soil adding opening is formed in the upper end of the box cover, a grinding mechanism for grinding soil is arranged at the upper end of the soil adding opening, the heating resistance wire is electrically connected with the heating control module, and the heating control module is electrically connected with the controller. The scheme is used for culturing potassium bacteria suitable for oilseed rape planting soil, at least three heating cavities are arranged in the culture box, a good heat preservation effect is achieved, humidity and temperature monitoring can be carried out, and it is guaranteed that the potassium bacteria grow in the environment with the proper temperature and humidity.
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Description

TECHNICAL FIELD

[0001] The utility model relates to the field of microorganism culture, specifically relates to a rapeseed planting soil's potassium solution bacteria culture device. BACKGROUND

[0002] Potassium is one of the important nutrient elements for crop growth, the nitrogen, phosphorus and potassium ratio in fertilizer is 1:0.4:0.16, and the lack of potassium in soil has become an important factor limiting crop yield and quality improvement.In agricultural production, chemical potassium fertilizer is often applied to provide potassium elements required for crop growth, but it can destroy soil structure and reduce organic matter content, which is not conducive to the sustainable development of agriculture.95% of potassium in soil is in the form of mineral potassium, which exists in potassium feldspar and mica, and cannot be directly absorbed and utilized by plants.Potassium solution bacteria are mainly silicate bacteria, which can decompose minerals and release potassium and other elements for plant utilization, convert ineffective potassium elements in soil into available potassium, and also have nitrogen fixation and phosphorus solution functions.Using microorganisms to obtain available potassium from potassium feldspar for crops has the advantages of low energy consumption, low cost, no pollution and simple method, and is considered as the most promising method for potassium extraction from potassium feldspar.

[0003] However, in the process of rapeseed planting, drought stress leads to inhibited root growth, early leaf senescence, and thus affects yield and rapeseed oil quality.Increasing potassium fertilizer can alleviate the adverse effects of drought to a certain extent, improve the distribution rate of potassium in leaves, increase the content of oleic acid and reduce the content of erucic acid in rapeseed, thereby improving seed quality.In order to reduce the amount of potassium fertilizer in the process of rapeseed planting, it is necessary to screen potassium solution bacteria suitable for rapeseed planting soil, and the premise of screening potassium solution bacteria is to carry out special culture.

[0004] The culture temperature of potassium solution bacteria needs to be set to 20 DEG C, 24 DEG C, 28 DEG C, 32 DEG C, 36 DEG C and 40 DEG C, the existing bacterial culture device has poor heat preservation effect, and the rapeseed planting soil as the culture medium for potassium solution bacteria has the characteristics of large soil tubers, which is not suitable for direct addition to the culture dish for culture. UTILITY MODEL CONTENT

[0005] In view of the above shortcomings of the prior art, the utility model provides a rapeseed planting soil potassium solution bacteria culture device.

[0006] In order to achieve the above-mentioned purposes, the utility model adopts the following technical scheme:

[0007] Provided is a rapeseed planting soil potassium solution bacteria culture device, which comprises an open-top culture box, a heating cavity is arranged on the side wall and the bottom of the culture box, a heating resistance wire is arranged in the heating cavity, a plurality of bearing tables are arranged on the inner wall of the culture box, the bearing tables are used for placing culture dishes, support blocks are arranged on both sides of the culture dishes and placed on the bearing tables, a box cover is arranged at the upper end of the culture box, a soil adding opening is arranged at the upper end of the box cover, a soil grinding mechanism is arranged at the upper end of the soil adding opening, the heating resistance wire is electrically connected with a heating control module, and the heating control module is electrically connected with a controller.

[0008] Further, the soil grinding mechanism comprises a machine body shell, the lower end of the machine body shell is open, a filter screen is arranged in the machine body shell, a crushing cylinder is arranged above the filter screen, a plurality of crushing teeth are arranged on the surface of the crushing cylinder, one end of the crushing cylinder is rotationally arranged on the inner wall of the machine body shell through a rotating shaft, the other end of the crushing cylinder is connected with a driving shaft extending to the outside of the machine body shell, the end of the driving shaft is connected with a crank handle, the driving shaft is connected with the machine body shell through a bearing, and the upper end of the machine body shell is provided with an opening, a sealing cover is arranged on the opening and threadedly connected with the opening.

[0009] Further, the inner wall of the lower end of the machine body shell is provided with an internal thread, the upper end of the soil adding opening is provided with an external thread, the machine body shell is threadedly connected with the soil adding opening, and the soil adding opening is provided with a sealing cover.

[0010] Further, a plurality of culture cavities are arranged on the culture dishes through partitions.

[0011] Further, the lower end of the support block is provided with a plurality of magnetic attraction columns, a plurality of sockets matched with the magnetic attraction columns are formed in the bearing table, and the inner wall of the socket is made of metal.

[0012] Further, a display screen is arranged on the outer wall of the culture box, a temperature sensor and a humidity sensor are mounted on the inner wall of the culture box, and the temperature sensor, the humidity sensor and the display screen are electrically connected with the controller.

[0013] Further, an observation window is arranged on the side wall of the culture box, and a baffle made of transparent material is arranged on the observation window.

[0014] The utility model discloses a beneficial effect is: the scheme is used to cultivate the potassium dissolving bacteria suitable for the rape planting soil, adds the rape planting soil as the culture medium to the petri dish, and then inoculates the potassium dissolving bacteria, makes the potassium dissolving bacteria breed on the rape planting soil, and the potassium dissolving bacteria suitable for the rape planting soil environment are screened out. Meanwhile, the incubator has at least three heating cavities, has good heat preservation effect, can also carry out humidity, temperature monitoring, guarantees that the potassium dissolving bacteria grow in the suitable temperature and humidity environment. The incubator can be provided with multilayer petri dishes, and the culture amount is increased, and the petri dish is placed in the incubator more stably through the magnetic attraction structure, and will not fall off because of the shaking of the incubator. The scheme is also provided with the matched grinding mechanism, can directly add the caked rape planting soil to the grinding mechanism and grind in the process of adding the culture medium, and the powder after grinding directly falls on the petri dish, convenient to use. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is the structure diagram of the potassium dissolving bacteria culture device for rape planting soil.

[0016] Figure 2 It is the structure diagram of the grinding mechanism.

[0017] Figure 3 It is the structure diagram of the petri dish.

[0018] Figure 4 It is the structure diagram of the bearing table.

[0019] Figure 5 It is the front view of the incubator.

[0020] Among them, 1, heating cavity, 2, petri dish, 3, incubator, 4, bearing table, 5, magnetic attraction column, 6, temperature sensor, 7, humidity sensor, 8, box cover, 9, add soil mouth, 10, sealing cover, 11, screw, 12, machine body shell, 13, filter screen, 14, handle, 15, broken cylinder, 16, opening, 17, airtight cover, 18, socket, 19, support block, 20, culture cavity. DETAILED DESCRIPTION

[0021] The specific embodiment of the utility model is described below, so that the person skilled in the art can understand the utility model, but it should be clear that the utility model is not limited to the scope of the specific embodiment, for the ordinary skilled person in the art, as long as various changes are in the spirit and scope of the utility model defined and determined in the attached claims, these changes are obvious, all the utility model creations using the utility model concept are in the protection.

[0022] Such as Figures 1-5As shown, a rapeseed planting soil potassium-lysing bacteria culture device, including an open upper end 16 culture box 3, the side wall and bottom of the culture box 3 is provided with a heating cavity 1, the surface of the heating cavity 1 is encapsulated by a porous mesh, a heating resistance wire is arranged in the heating cavity 1, the heating resistance wire is electrified to realize heat preservation inside the culture box 3, a plurality of bearing tables 4 are arranged on the inner wall of the culture box 3, the plurality of bearing tables 4 are distributed on both sides of the inner wall of the culture box 3, the bearing tables 4 are used for placing culture dishes 2, both sides of the culture dishes 2 are provided with support blocks 19 placed on the bearing tables 4, an upper end of the culture box 3 is provided with a box cover 8, the box cover 8 is fixed on the culture box 3 by screws 11 and can be detached, an upper end of the box cover 8 is provided with a soil adding port 9, an upper end of the soil adding port 9 is provided with a soil grinding mechanism for grinding soil, the heating resistance wire is electrically connected with a heating control module, and the heating control module is electrically connected with a controller.

[0023] The present scheme is used for culturing potassium-lysing bacteria suitable for rapeseed planting soil, rapeseed planting soil is added into the culture dishes 2 as culture medium, and the potassium-lysing bacteria is inoculated, so that the potassium-lysing bacteria is multiplied on the rapeseed planting soil, and the potassium-lysing bacteria suitable for the rapeseed planting soil environment is screened out. Meanwhile, the culture box 3 has at least three heating cavities 1, and has good heat preservation effect.

[0024] In the embodiment, the grinding mechanism includes a machine body shell 12, the lower end of the machine body shell 12 is open, a filter screen 13 is arranged in the machine body shell 12, a crushing cylinder 15 is arranged above the filter screen 13, a plurality of crushing teeth are arranged on the surface of the crushing cylinder 15, one end of the crushing cylinder 15 is rotatably arranged on the inner wall of the machine body shell 12 through a rotating shaft, the other end of the crushing cylinder 15 is connected with a driving shaft extending outside the machine body shell 12, the end of the driving shaft is connected with a crank 14, the driving shaft is connected with the machine body shell 12 through a bearing, an opening 16 is arranged at the upper end of the machine body shell 12, a sealing cover 17 is arranged on the opening 16, and the sealing cover 17 is threadedly connected with the opening 16.

[0025] By arranging the matching grinding mechanism, the caked rapeseed planting soil can be directly added into the grinding mechanism for grinding during the addition of the culture medium, and the powder after grinding can directly fall on the culture dishes 2, so that the use is convenient. After grinding, the grinding mechanism can be removed, so as to reduce the weight of the culture box 3. If the rapeseed planting soil is not used as the culture medium of the potassium-lysing bacteria in the embodiment, but a formula culture medium is added into the culture dishes 2, the grinding mechanism can also be used as a mixing device to mix the formula culture medium uniformly and then discharge the formula culture medium into the culture dishes 2.

[0026] In the embodiment, the lower end inner wall of the machine body shell 12 is provided with an internal thread, the upper end of the soil adding port 9 is provided with an external thread, the machine body shell 12 is threadedly connected with the soil adding port 9, and the soil adding port 9 is provided with a sealing cover 10. The grinding mechanism is convenient to disassemble and assemble, and after the grinding mechanism is disassembled, the soil adding port 9 is blocked by the sealing cover 10.

[0027] In this embodiment, the culture dish 2 is provided with a plurality of culture cavities 20 through the partition, and multiple types of culture medium culture can be carried out to screen the culture medium type suitable for the growth of potassium-dissolving bacteria, and the culture mediums are compared with each other.

[0028] In this embodiment, the lower end of the support block 19 is provided with a plurality of magnetic columns 5, and the bearing table 4 is provided with a plurality of sockets 18 matched with the magnetic columns 5, and the inner wall of the socket 18 is made of metal. Multiple layers of culture dishes 2 can be arranged in the incubator 3, which increases the culture amount, and the culture dishes 2 are placed in the incubator 3 more stably through the magnetic structure and will not fall off due to the shaking of the incubator 3. At the same time, the culture dishes 2 are also convenient to take and place.

[0029] In this embodiment, the outer wall of the incubator 3 is provided with a display screen, the inner wall of the incubator 3 is provided with a temperature sensor 6 and a humidity sensor 7, and the temperature sensor 6, the humidity sensor 7 and the display screen are electrically connected with the controller. The humidity and temperature can also be monitored to ensure that the potassium-dissolving bacteria grow in a suitable temperature and humidity environment, and the display screen can display the temperature and humidity data in the incubator 3. In this embodiment, the controller uses a PCB board loaded with a C51 single-chip microcomputer, the heating control module uses an AZBIL temperature control module NX-D15NT4C20, the temperature sensor 6 uses an FT-H10 type infrared temperature sensor, and the humidity sensor 7 uses a DHT11 humidity sensor.

[0030] In this embodiment, the sidewall of the incubator 3 is provided with an observation window, and the observation window is provided with a baffle made of transparent material. During the closed culture process, the internal situation can be observed at any time.

Claims

1. A device for cultivating potassium-solubilizing bacteria in rapeseed planting soil, characterized in that, The application discloses a culture box with an open upper end, wherein a heating cavity is arranged on the side wall and the bottom of the culture box, a heating resistance wire is arranged in the heating cavity, a plurality of bearing tables are arranged on the inner wall of the culture box and are used for placing culture dishes, support blocks are arranged on the two sides of the culture dishes and are placed on the bearing tables, a box cover is arranged on the upper end of the culture box, a soil adding opening is arranged on the upper end of the box cover, a grinding mechanism for grinding soil is arranged on the upper end of the soil adding opening, the heating resistance wire is electrically connected with a heating control module, and the heating control module is electrically connected with a controller.

2. The oilseed rape planting soil's potassium-dissolving bacteria culture device according to claim 1, characterized in that, The grinding mechanism comprises a machine body shell, the lower end of the machine body shell is open, a filter screen is arranged in the machine body shell, a crushing cylinder is arranged above the filter screen, a plurality of crushing teeth are arranged on the surface of the crushing cylinder, one end of the crushing cylinder is rotationally arranged on the inner wall of the machine body shell through a rotating shaft, the other end of the crushing cylinder is connected with a driving shaft which extends to the outside of the machine body shell, the end of the driving shaft is connected with a handle, the driving shaft is connected with the machine body shell through a bearing, an opening is arranged on the upper end of the machine body shell, a sealing cover is arranged on the opening, and the sealing cover is threadedly connected with the opening.

3. The oilseed rape planting soil's potassium-dissolving bacteria culture device according to claim 2, characterized in that, The inner wall of the lower end of the machine body shell is provided with an internal thread, the upper end of the soil adding opening is provided with an external thread, the machine body shell is threadedly connected with the soil adding opening, and the soil adding opening is provided with a sealing cover.

4. The oilseed rape planting soil's potassium-dissolving bacteria culture device according to claim 1, characterized in that, A plurality of culture cavities are arranged on the culture dish through a partition plate.

5. The oilseed rape planting soil's potassium-dissolving bacteria culture device according to claim 1, characterized in that, The lower end of the support block is provided with a plurality of magnetic attraction columns, a plurality of sockets matched with the magnetic attraction columns are formed in the bearing table, and the inner wall of the socket is made of metal.

6. The oilseed rape planting soil's potassium-dissolving bacteria culture device according to claim 1, characterized in that, A display screen is arranged on the outer wall of the culture box, a temperature sensor and a humidity sensor are mounted on the inner wall of the culture box, and the temperature sensor, the humidity sensor and the display screen are electrically connected with the controller.

7. The oilseed rape planting soil's potassium-dissolving bacteria culture device according to claim 1, characterized in that, An observation window is arranged on the side wall of the culture box, and a baffle made of transparent material is arranged on the observation window.