Cell co-culture device
By designing the combination of flow channels, docking channels, and channel channels in the cell co-culture device, the problem of unclear boundaries in cell co-culture was solved, enabling convenient observation and research of cell contact reactions.
Patent Information
- Application Number
- CN202422899767.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-27
- Publication Date
- 2026-01-09
- Estimated Expiration
- 2034-11-27
AI Technical Summary
In existing cell co-culture methods, the boundary lines after different cells are mixed are not clear enough, making it difficult to observe the mutual influence and individual effects between cells.
A cell co-culture device was designed, including an incubator, a placement tank, an outer sleeve, and a culture dish. Through the cooperation of a flow channel, a docking channel, and a channel, the mixing and separation of cell solutions can be achieved, facilitating the observation of cell contact reactions.
It enables convenient observation of reactions when different cells come into contact, allowing for the study of contact reactions in various cell cultures, and is easy to operate and observe.
Smart Images
Figure CN223780268U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of cell culture, and in particular to a cell co-culture device. BACKGROUND
[0002] The cell co-culture technology is a technology of co-culturing two or more cells in a unified environment. Compared with single cell monolayer culture, the cell co-culture technology has the advantage of better reflecting the in-vivo environment. At present, the existing co-culture methods include direct contact co-culture and indirect contact co-culture. The direct contact co-culture can solve the co-culture state of multiple cells, but the cells of different tissue origins are mixed together for culture, and cannot be independently distinguished. During the growth process, different cells contact and influence each other, causing the expression of the respective functions of the mixed cells or causing changes in the properties of various cells. For the study of the mutual influence and respective effects of cells in the later stage, it is often difficult to distinguish or lose the true response.
[0003] However, in actual application, when the reaction of the contact of different cells after culture is studied, the mixed different cells have less obvious boundaries and are not easy to observe.
[0004] Therefore, the skilled in the art provides a cell co-culture device to solve the problems in the background art. CONTENT OF THE INVENTION
[0005] In order to solve the problems in the background art, the present application provides a cell co-culture device.
[0006] The cell co-culture device provided by the present application adopts the following technical scheme:
[0007] The cell co-culture device comprises a culture box, a placing groove is arranged in the culture box, an outer sleeve is arranged in the placing groove, and a culture dish is arranged in the inner part of the outer sleeve. The placing groove comprises a center groove arranged at the center and four edge grooves arranged at the periphery. The outer sleeve placed in the center groove is a center sleeve, the outer sleeve placed in the edge groove is an edge sleeve, and a channel groove is arranged between the center groove and the four corresponding edge grooves. The corresponding positions of the center sleeve, the edge sleeve and the channel groove are also provided with a butt joint groove, and four flow grooves are also arranged on the surface of the culture dish.
[0008] Preferably, a square groove is arranged at the center of the bottom wall of the placing groove, and a square block matched with the square groove is arranged on the bottom of the outer sleeve.
[0009] Preferably, a magnetic attraction piece is further embedded at the center of the bottom wall of the outer sleeve, and a magnetic attraction block protruding outward is arranged at the center of the bottom of the culture dish.
[0010] Preferably, the height of the culture dish is greater than the height of the outer sleeve, and a knob is sleeved on the top opening of the culture dish, the width of the flow-through groove on the culture dish is less than the width of the butt joint groove on the outer sleeve, the width of the butt joint groove on the outer sleeve is less than the width of the channel groove, and the bottom walls of the flow-through groove, the butt joint groove and the channel groove are flush.
[0011] Preferably, a box cover is also hingedly installed on the opening of the incubator, and a ring-shaped fluorescent lamp is arranged on the inner wall of the incubator.
[0012] In summary, the present application has the following beneficial technical effects:
[0013] 1. In the present application, the culture dish is rotated to stagger the flow-through groove and the butt joint groove, so that the cells in the culture dish can be cultured, and then the flow-through groove and the butt joint groove are aligned with the channel groove, so that the cells in the two culture dishes can flow into the channel groove and mix, which can facilitate observation of the reaction of the two cells when they contact, and is relatively easy to operate.
[0014] 2. In the present application, five culture dishes and five placing grooves are provided, different cells can be cultured in each culture dish, and through the cooperation of the channel groove, contact reaction research of multiple cell cultures can be carried out, which is relatively convenient. BRIEF DESCRIPTION OF DRAWINGS
[0015] Figure 1 is a structural schematic view of the incubator of the present application;
[0016] Figure 2 is a structural schematic view of the placing groove in the present application;
[0017] Figure 3 is a structural schematic view of the upper perspective view of the outer sleeve in the present application;
[0018] Figure 4 is a structural schematic view of the lower perspective view of the outer sleeve in the present application;
[0019] Figure 5 is a structural schematic view of the upper perspective view of the culture dish in the present application;
[0020] Figure 6 is a structural schematic view of the lower perspective view of the culture dish in the present application.
[0021] BRIEF DESCRIPTION OF DRAWINGS: 1, incubator; 2, placing groove; 21, center groove; 22, edge groove; 23, channel groove; 25, square groove; 3, outer sleeve; 31, center sleeve; 32, edge sleeve; 33, butt joint groove; 34, magnetic attraction piece; 35, square block; 4, culture dish; 41, flow-through groove; 42, knob; 43, magnetic attraction block. DETAILED DESCRIPTION
[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0023] Example 1
[0024] like Figures 1-6 As shown, this application discloses a cell co-culture device, including an incubator 1. The incubator 1 is hinged with a lid 11. An annular fluorescent lamp 12 is provided on the inner wall of the incubator 1. The incubator 1 is provided with a placement groove 2. An outer sleeve 3 is provided in the placement groove 2. A culture dish 4 is placed inside the outer sleeve 3. The height of the culture dish 4 is greater than the height of the outer sleeve 3. A knob 42 is fitted on the top opening of the culture dish 4. The placement groove 2 includes a central groove 21 and four edge grooves 22. The outer sleeve 3 placed in the central groove 21 is a central sleeve 31, and the outer sleeve 3 placed in the edge grooves 22 is an edge sleeve 32. A channel groove 23 is provided between the central groove 21 and the four corresponding edge grooves 22. A docking groove 33 is also provided at the position corresponding to the channel groove 23 of the central sleeve 31 and the edge sleeve 32. Four flow grooves 41 are also provided on the surface of the culture dish 4.
[0025] Specifically, the width of the flow channel 41 on the culture dish 4 is smaller than the width of the docking groove 33 on the outer sleeve 3, and the width of the docking groove 33 on the outer sleeve 3 is smaller than the width of the channel groove 23. The bottom walls of the flow channel 41, docking groove 33 and channel groove 23 are flush. After the outer sleeve 3 is placed in the placement groove 2, the docking groove 33 on the outer sleeve 3 is aligned with the channel groove 23. At this time, the flow channel 41 and docking groove 33 on the culture dish 4 are staggered, so that cells can be cultured in the culture dish 4. Afterwards, when the flow channel 41 is rotated to align with the docking groove 33 and channel groove 23, the cells in the corresponding two culture dishes 4 can flow into the channel groove 23 to mix. This makes it easy to observe the reaction when the two types of cells come into contact and is relatively easy to operate.
[0026] like Figure 2 , Figure 4 As shown, a square groove 25 is provided at the center of the bottom wall of the placement groove 2, and a block 35 that matches the square groove 25 is installed on the bottom of the outer sleeve 3. By cooperating with the square groove 25, the outer sleeve 3 can be confined within the placement groove 2, ensuring that the docking groove 33 is always aligned with the channel groove 23.
[0027] like Figure 3 , Figure 6As shown, the bottom wall center of the outer sleeve 3 is also embedded with a magnetic sheet 34, and the bottom center of the culture dish 4 is installed with an outward convex magnetic block 43, through the cooperation of the magnetic sheet 34 and the magnetic block 43, the culture dish 4 can be adsorbed inside the outer sleeve 3, the culture dish 4 is kept close to the inner wall of the outer sleeve 3, and the cell solution in the culture dish 4 is prevented from flowing into the outer sleeve 3.
[0028] The standard parts used in the utility model can be purchased from the market, the special-shaped parts can be ordered according to the description and the drawings, the specific connection mode of each part adopts the conventional means such as bolts, rivets and welding in the prior art, the machinery, parts and equipment adopt conventional models in the prior art, and the circuit connection adopts the conventional connection mode in the prior art, and details are not described herein.
[0029] Meanwhile, the contents not described in detail in the specification all belong to the prior art known by the person skilled in the art.
[0030] The implementation principle of the cell co-culture device in the embodiment of the application is as follows:
[0031] In use, the culture dish 4 is first placed in the outer sleeve 3, then the center sleeve 31 is placed in the center groove 21, and the edge sleeve 32 is placed in the edge groove 22, because the block 35 at the bottom of the outer sleeve 3 can cooperate with the square groove 25 in the placing groove 2, the outer sleeve 3 is limited in the placing groove 2, and the butt joint groove 33 on the outer sleeve 3 is always aligned with the corresponding channel groove 23 on the placing groove 2, at this time, the culture dish 4 can be rotated in the outer sleeve 3 by the knob 42, and when the flow-through groove 41 on the culture dish 4 is misaligned with the butt joint groove 33 on the outer sleeve 3, the cell solution and the nutrient solution can be added in the culture dish 4 for culture;
[0032] When different cell contact reactions need to be observed, the knob 42 can be rotated, the flow-through groove 41 on the culture dish 4 is aligned with the butt joint groove 33 and the channel groove 23 on the outer sleeve 3, at this time, the cell solutions in the two culture dishes 4 connected in communication flow into the channel groove 23 until contact, at this time, the reaction of the two kinds of cells when contacting can be conveniently observed, and the operation is relatively easy;
[0033] In addition, in order to prolong the time of different cell contact reactions, an appropriate amount of nutrient solution can be added in the channel groove 23 in advance, when the flow-through groove 41 is aligned with the channel groove 23 by rotating the culture dish 4, the nutrient solution in the channel groove 23 flows to the two culture dishes 4, and then the cells in the culture dishes 4 grow into the channel groove 23 until contact, and the reaction of different cells when contacting can be observed in more detail.
[0034] The basic principle, main features and advantages of the present application are shown and described above. It should be understood by those skilled in the art that the present application is not limited by the above examples, and the above examples and descriptions in the specification are only preferred examples of the present application and are not intended to limit the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the claimed present application. The scope of protection of the present application is defined by the appended claims and their equivalents.
Claims
1. A cell co-culture device, comprising a culture box (1), wherein a placing groove (2) is arranged inside the culture box (1), an outer sleeve (3) is arranged in the placing groove (2), and a culture dish (4) is sleeved inside the outer sleeve (3), characterized in that, The placing groove (2) comprises a center groove (21) arranged at the center and four edge grooves (22) arranged at the periphery, the outer sleeve (3) placed in the center groove (21) is a center sleeve (31), the outer sleeve (3) placed in the edge groove (22) is an edge sleeve (32), and a passage groove (23) is arranged between the center groove (21) and the corresponding edge groove (22), the center sleeve (31) and the edge sleeve (32) are also provided with a butt joint groove (33) at the corresponding position of the passage groove (23), and the surface of the culture dish (4) is also provided with four flow grooves (41).
2. The cell co-culture device of claim 1, wherein: The bottom wall of the placing groove (2) is provided with a square groove (25) at the center, and the bottom of the outer sleeve (3) is provided with a square block (35) matched with the square groove (25).
3. The cell co-culture device of claim 1, wherein: The bottom wall of the outer sleeve (3) is also embedded with a magnetic piece (34) at the center, and the bottom center of the culture dish (4) is provided with an outwardly convex magnetic block (43).
4. The cell co-culture device of claim 1, wherein: The height of the culture dish (4) is greater than the height of the outer sleeve (3), and the top opening of the culture dish (4) is provided with a knob (42), the width of the flow groove (41) on the culture dish (4) is less than the width of the butt joint groove (33) on the outer sleeve (3), the width of the butt joint groove (33) on the outer sleeve (3) is less than the width of the passage groove (23), and the bottom wall of the flow groove (41), the butt joint groove (33) and the passage groove (23) are flush.
5. The cell co-culture device of claim 1, wherein: The opening of the incubator (1) is also hingedly provided with a cover (11), and the inner wall of the incubator (1) is provided with a ring-shaped fluorescent lamp (12).