Integrated device for sorting and detecting lung cancer cells

Abstract

The utility model relates to the field of lung cancer cells, in particular to a lung cancer cell sorting and detecting integrated device which comprises a mounting frame, a fixing frame, a flowing chamber, a diffusion plate, a controller, a temperature detecting tube, a heating wire ring, an isolating plate, a connecting tube and a refrigerating module, a flow chamber used for sorting lung cancer cells is fixedly connected to the upper end surface of the mounting frame, a fixing frame connected with the flow chamber is arranged at the upper end of the mounting frame, a refrigeration module is mounted in the center inside the rear end of the mounting frame, and a diffusion plate penetrating through the mounting frame and used for keeping internal constant temperature is mounted at the upper end of the refrigeration module. According to the lung cancer cell sorting and detecting integrated device disclosed by the utility model, through the refrigeration module, the heating wire ring and the temperature detecting tube, the constant temperature in the sorting process is ensured, which is of great importance to keeping the cell activity and the reliability of an experimental result.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of lung cancer cells, especially lung cancer cell sorting and detection integrated device. BACKGROUND

[0002] Cell sorter refers to a tool for realizing cell sorting and further manipulating single cell culture, and the cell sorter belongs to a kind of medical apparatus, with high cell activity, high precision and other advantages, and is widely applied in cell therapy or biomedical field, and lung cancer cell detection device is a kind of instrument or system specially designed for identifying, separating and analyzing lung cancer cells, which plays a crucial role in cancer research, diagnosis, treatment monitoring and personalized medicine.

[0003] Traditional cell sorting method often needs multiple independent devices, which not only increases cost, but also causes pollution or loss in sample transfer process, and in traditional multi-device process, it is difficult to maintain constant temperature conditions in the whole process, and the risk of exposure to external environment may cause pollution or cell loss.

[0004] Therefore, aiming at the above-mentioned traditional cell sorting method often needs multiple independent devices, and in traditional multi-device process, it is difficult to maintain constant temperature conditions in the whole process, lung cancer cell sorting and detection integrated device reduces the demand for manual operation through integrated design, reduces human error, improves work efficiency, makes large-scale sample processing possible, and ensures constant temperature in the sorting process through refrigeration module, heating wire ring and temperature detection tube, which is crucial for maintaining cell activity and reliability of experimental results. UTILITY MODEL CONTENTS

[0005] In order to overcome the problem that traditional cell sorting method often needs multiple independent devices, and in traditional multi-device process, it is difficult to maintain constant temperature conditions in the whole process.

[0006] The technical scheme of the utility model is: lung cancer cell sorting and detection integrated device, including installation frame, fixed support, flow chamber, diffusion plate, controller, temperature detection tube, heating wire ring, isolation plate, connecting pipe and refrigeration module;The flow chamber for sorting lung cancer cells is fixedly connected to the upper surface of the installation frame, the fixed support connected with the flow chamber is arranged on the upper end of the installation frame, the refrigeration module is installed in the center of the rear end of the installation frame, the diffusion plate for maintaining internal constant temperature is installed on the upper end of the refrigeration module and penetrates the installation frame, the connecting pipe is connected between the diffusion plate and the refrigeration module, the heating wire ring is sleeved outside the connecting pipe, the heating wire ring is sleeved outside the heating wire ring for blocking the heat diffusion of the heating wire ring to the isolation plate, the controller for adjusting the temperature to make it constant temperature is electrically connected to the outside of the heating wire ring and the refrigeration module, and the temperature detection tube penetrating the diffusion plate is arranged on the center of the upper end of the controller.

[0007] Preferably, the installation frame serves as the basic structure of the entire device, providing mechanical support and fixing other components, the fixing frame connects the flow chamber with the installation frame, ensuring that the flow chamber remains stable during operation, the flow chamber serves as the core area of cell sorting, and the separation of cells is achieved through fluid mechanics or in combination with other physical and chemical methods, the controller controls the operation of the entire system, including temperature adjustment, monitoring of the sorting process, etc., the temperature detection tube monitors the temperature in the flow chamber in real time, the heating wire coil surrounds the connecting pipe for heating the fluid or gas passing through, the refrigeration module and the heating wire coil work together to transmit the fluid or gas through the connecting pipe and monitor the temperature in real time by the temperature detection tube, and the controller automatically adjusts the heating and refrigeration power according to the temperature feedback to ensure that the temperature in the flow chamber remains constant at all times.

[0008] Preferably, the controller is installed on both sides of the lower end and is fixedly connected with the connecting frame of the installation frame, the surface of the installation frame is provided with a cabinet door, the flow chamber is fixedly connected with the mounting frame at the edge line, and the upper surface of the mounting frame is annularly provided with a cell sorting channel at the center.

[0009] Preferably, the cell separation cavity is installed on the upper surface of the mounting frame near the corner, the mounting frame is provided with a fixing block near the center of the edge line on the upper surface, and the inlet is fixedly connected to the upper end of the fixing block.

[0010] Preferably, the laser beam is vertically installed on the rear surface of the fixing block, and the inclined plate is installed inside the flow chamber near the edge line.

[0011] Preferably, the connecting channel is sequentially penetrated from left to right through the flow chamber and the inclined plate at the edge line away from the fixing block on the upper surface of the mounting frame, and the separation module is installed on the upper surface of the mounting frame at the upper end of the connecting channel.

[0012] Preferably, the detection module corresponding to the connecting channel is sequentially installed from left to right on one side of the fixing frame close to the flow chamber, the display screen is installed at the center of the side of the fixing frame away from the detection module, and the placing cabinet is installed between the fixing frame and the installation frame.

[0013] Preferably, the receiving plate is installed at the center of the placing cabinet, the pulling plate is arranged at the center of the surface of the receiving plate, and a plurality of groups of collection test tubes are sequentially installed in the receiving plate.

[0014] Preferably, the placing cabinet is provided with a sliding block on both sides, and the sliding block is slidingly connected with the guide rail on both sides of the installation frame.

[0015] The utility model discloses a beneficial effect: compared with traditional lung cancer cell sorting and detection integrated device, this new type ensures the stability and positional accuracy of all internal components through the installation frame, reduces the influence of vibration and other external factors on the experiment, and the fixed frame prevents the displacement of the flow chamber in the sorting process due to fluid power or other external force, guarantees the continuity and precision of the sorting process, realizes the high-precision sorting of lung cancer cells through the integrated laser beam system and flow chamber design, ensures the temperature constancy in the sorting and detection process through the accurate temperature control system, maintains cell activity, reduces artificial intervention, improves work efficiency, reduces error rate, completes cell sorting and detection on a platform, simplifies the work flow, saves time and resources. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 The whole structure schematic diagram of the lung cancer cell sorting and detection integrated device of the utility model is shown.

[0017] Figure 2 The unfolded structure schematic diagram of the storage plate of the lung cancer cell sorting and detection integrated device of the utility model is shown.

[0018] Figure 3 The cell separation cavity structure schematic diagram of the lung cancer cell sorting and detection integrated device of the utility model is shown.

[0019] Figure 4 The collection test tube structure schematic diagram of the lung cancer cell sorting and detection integrated device of the utility model is shown.

[0020] Figure 5 The refrigeration module structure schematic diagram of the lung cancer cell sorting and detection integrated device of the utility model is shown.

[0021] The utility model discloses a beneficial effect: compared with traditional lung cancer cell sorting and detection integrated device, this new type ensures the stability and positional accuracy of all internal components through the installation frame, reduces the influence of vibration and other external factors on the experiment, and the fixed frame prevents the displacement of the flow chamber in the sorting process due to fluid power or other external force, guarantees the continuity and precision of the sorting process, realizes the high-precision sorting of lung cancer cells through the integrated laser beam system and flow chamber design, ensures the temperature constancy in the sorting and detection process through the accurate temperature control system, maintains cell activity, reduces artificial intervention, improves work efficiency, reduces error rate, completes cell sorting and detection on a platform, simplifies the work flow, saves time and resources. DETAILED DESCRIPTION

[0022] The utility model will be further explained below in connection with the drawings and examples.

[0023] Please refer to Figure 1 - Figure 4The utility model provides a kind of embodiment: lung cancer cell sorting and detection integrated device, including mounting frame 1, fixed frame 2, flow chamber 3, diffusion plate 22, controller 23, temperature detection tube 24, heating wire ring 25, isolation plate 26, connecting pipe 27 and refrigeration module 28;Flow chamber 3 for sorting lung cancer cells is fixedly connected on the upper end surface of mounting frame 1, fixed frame 2 connected with flow chamber 3 is equipped on the upper end of mounting frame 1, refrigeration module 28 is installed in the inside center of mounting frame 1 rear end, diffusion plate 22 for keeping internal constant temperature is installed on the upper end of refrigeration module 28 and penetrates mounting frame 1, connecting pipe 27 is connected between diffusion plate 22 and refrigeration module 28, heating wire ring 25 is sleeved on the outside of connecting pipe 27, heating wire ring 25 is sleeved on the outside of connecting pipe 27 for blocking heating wire ring 25 heat diffusion to isolation plate 26, controller 23 for adjusting temperature to make it constant temperature is electrically connected on the outside of heating wire ring 25 and refrigeration module 28, temperature detection tube 24 is equipped on the upper end center of controller 23 and penetrates diffusion plate 22.

[0024] Please refer to Figure 1 Figure 2 In the embodiment, controller 23 lower end both sides are equipped with the connecting frame 29 fixedly connected with mounting frame 1, the surface of mounting frame 1 is equipped with cabinet door 4, flow chamber 3 margin is fixedly connected with mounting frame 5, annular cell sorting channel 17 is opened in the upper surface center of mounting frame 5, connecting frame 29 is firmly fixed on mounting frame 1 with controller 23, the mechanical stability of entire system is enhanced, the displacement or damage caused by vibration or external force is reduced, the design of connecting frame 29 makes controller 23 can be conveniently detached from mounting frame 1, is convenient for maintenance, cleaning or replacement parts, cabinet door 4 can effectively block dust, moisture and other pollutants from entering the inside of device, keep the inside environment clean, prolong the service life of equipment, the design of cabinet door 4 makes that user can easily open and close, it is convenient to carry out sample loading, the replacement of collection test tube 19 and daily maintenance, mounting frame 5 upper surface is installed near corner with cell separation cavity 15, mounting frame 5 upper surface center is installed near margin with fixed block 12, fixed block 12 upper end center is fixedly connected with inlet 13, install cell separation cavity 15 near corner, can optimize fluid path, reduce the turbulence and disturbance of fluid when entering flow chamber 3, ensure that cell can enter sorting area smoothly, by reasonable fluid design, can make cell rapidly disperses after entering separation cavity, avoid cell aggregation or block, to improve sorting efficiency and precision, the position design of cell separation cavity 15 makes that sample can be preliminary treated or pre-sorted before entering flow chamber 3, reduce the complexity of subsequent sorting steps, fixed block 12 provides stable support for inlet 13, ensure that inlet 13 does not displace or sway during operation, guarantee the accuracy and stability of sample loading.

[0025] Please refer to​Figure 2 - Figure 3 In this embodiment, a laser beam 14 is vertically mounted on the rear surface of the fixing block 12, and an inclined plate 10 is installed inside the flow chamber 3 near the edge. Vertically mounting the laser beam 14 on the rear surface of the fixing block 12 ensures that the emission direction of the laser beam 14 is perpendicular to the cell flow path, thereby achieving highly precise cell positioning and sorting. This design reduces the angular deviation between the laser beam 14 and the cells, improving the accuracy and resolution of sorting. The vertically mounted laser beam 14 provides a clear optical signal as cells pass through, reducing misjudgments caused by angle issues and ensuring that only cells meeting specific conditions are successfully sorted. The laser beam 14 can maximize the excitation of fluorescent markers or other optical properties in the cells, improving detection sensitivity and signal-to-noise ratio, and helping to identify cells with weak signals or low expression. The upper surface of the mounting bracket 5 is far... A connecting channel 11 runs from left to right through the flow chamber 3 and the inclined plate 10 at the edge of the fixed block 12. A separation module 16 is installed on the upper surface of the mounting frame 5 at the upper end of the connecting channel 11. The design of the connecting channel 11 makes the flow of cells from the flow chamber 3 to the separation module 16 smoother, reducing turbulence and resistance in the flow chamber 3 or narrow areas. This design helps to maintain the integrity and activity of cells and avoid cell damage caused by mechanical stress. The combined design of the connecting channel 11 and the separation module 16 allows the sorted cells to be quickly guided to a specific collection area, reducing the residence time of cells in the flow chamber 3 and reducing the risk of cross-contamination. Each connecting channel 11 can guide cells to different separation modules 16 according to different sorting conditions, realizing multi-channel parallel sorting and improving the sorting speed and throughput.

[0026] Please see Figure 3 - Figure 4In the embodiment, the fixed frame 2 is installed with detection modules 9 corresponding to the connecting channels 11 from left to right on one side close to the flow chamber 3, and a display screen 18 is installed at the center of the side of the fixed frame 2 away from the detection modules 9, a placing cabinet 7 is installed between the fixed frame 2 and the installation frame 1, and the plurality of detection modules 9 correspond one-to-one to the connecting channels 11, which can realize synchronous detection of cells in different sorting paths. This design significantly improves the detection efficiency and reduces the time required for a single module to process a large number of samples. Each detection module 9 works independently, avoiding mutual interference between multiple detection signals, ensuring the accuracy and reliability of the detection results. The display screen 18 provides an intuitive operation interface, allowing users to view key information such as sorting and detection progress, temperature changes, and cell characteristics in real time, facilitating experimental monitoring and adjustment. All important operations and settings can be performed through the display screen 18, reducing the time spent switching between different devices and improving work efficiency. The receiving plate 6 is installed at the center of the inside of the placing cabinet 7, and the pull plate 8 is provided at the center of the surface of the receiving plate 6. A plurality of groups of collection test tubes 19 are installed horizontally in the receiving plate 6. The receiving plate 6 is located at the center of the placing cabinet 7, providing a centralized and orderly sample storage space. Each receiving plate 6 can be designed with multiple layers or slots to facilitate the classification and storage of different types of samples or experimental materials, avoiding confusion. The design of the pull plate 8 allows users to easily pull out and push back the receiving plate 6, making it convenient to take and place samples. The design of the pull plate 8 allows users to quickly access the required samples or materials without completely opening the cabinet door 4, reducing operation time and improving work efficiency. The sliding blocks 20 installed on both sides of the placing cabinet 7 provide stable support for the receiving plate 6, ensuring smooth movement during pulling out and pushing back, avoiding sample dropping or damage due to shaking or tilting. The sliding blocks 20 are installed on both sides of the placing cabinet 7, and the guide rails 21 are slidingly connected to the outside of the sliding blocks 20 inside the installation frame 1. The sliding connection design of the guide rails 21 and the sliding blocks 20 ensures the stability of the receiving plate 6 during pulling out and pushing back. A high-quality guide rail 21 system can reduce friction and shaking, avoiding sample damage or leakage due to mechanical stress. The guide rail 21 system provides precise positioning function, ensuring that the receiving plate 6 can be accurately aligned with the opening of the placing cabinet 7 each time, facilitating user operation and reducing inconvenience caused by positional deviation. The receiving plate 6 is provided with a plurality of collection test tubes 19 corresponding to the connecting channels 11 from left to right at the center. This layout allows each sorted cell sample to be accurately guided into the corresponding test tube, avoiding sample confusion or loss.

[0027] In operation, according to experimental requirements, start the heating coil 25 or the refrigeration module 28 to make the flow chamber 3 reach and maintain the set temperature, the temperature detection tube 24 monitors the temperature change in real time and feeds back to the controller 23 for adjustment, the prepared cell sample is injected into the flow chamber 3 through the inlet 13, which can be in liquid form and introduced into the flow chamber 3 by a pump or other means, in the flow chamber 3, by controlling the speed and direction of the fluid, the preliminary separation of cells is realized. Different types of cells show different behaviors in the fluid due to the differences in size, shape and density. The laser beam 14 irradiates the cells in the flow, and according to the optical properties of the cells, the laser beam 14 can identify specific lung cancer cells and separate them from other cells by electric charge or other physical means. The inclined plate 10 inside the flow chamber 3 helps guide the sorted cells to a specific collection channel, reducing cross contamination. The sorted cells enter the detection module 9 through the connecting channel 11. The detection module 9 can use multiple technologies such as fluorescence detection, conductivity measurement, image analysis, etc. to analyze and classify the cells in detail. The detection results are transmitted to the controller 23 through the sensor and displayed on the display screen 18. Users can view the progress of sorting and detection in real time and obtain detailed cell information. The sorted and detected cells are guided to different collection tubes 19 by the separation module 16. Each collection tube 19 corresponds to a specific type of cell, facilitating subsequent processing and analysis.

[0028] Through the above steps, through the integrated flow chamber 3, laser beam 14 system and detection module 9, highly precise sorting of lung cancer cells can be achieved, improving the accuracy of research and diagnosis. Not only can the cells be sorted, but also the cells can be detected on the same platform, simplifying the workflow, saving time and resources. The storage plate 6 of the collection tube 19 allows users to easily manage and store the sorted cell samples, improving the organization and efficiency of the experiment. Through the refrigeration module 28, heating coil 25 and temperature detection tube 24, the temperature during the sorting process is ensured to be constant, which is crucial for maintaining cell activity and the reliability of experimental results. The problem of traditional cell sorting methods often requiring multiple independent devices and being difficult to maintain constant temperature conditions throughout the process has been solved.

Claims

1. A device for integrated sorting and detection of lung cancer cells, comprising a mounting frame (1); characterized in that: Also include fixed frame (2), flow chamber (3), diffusion plate (22), controller (23), temperature detection tube (24), heating coil (25), isolation plate (26), connecting pipe (27) and refrigeration module (28); the upper end surface of the mounting frame (1) is fixedly connected with the flow chamber (3) for sorting lung cancer cells, the upper end of the mounting frame (1) is provided with the fixed frame (2) connected with the flow chamber (3), the mounting frame (1) is internally provided with the refrigeration module (28) at the rear end, the refrigeration module (28) is provided with the diffusion plate (22) penetrating through the mounting frame (1) for keeping constant temperature, the connecting pipe (27) is connected between the diffusion plate (22) and the refrigeration module (28), the heating coil (25) is sleeved outside the connecting pipe (27), the heating coil (25) is sleeved outside the isolation plate (26) for blocking the heat diffusion of the heating coil (25), the heating coil (25) and the refrigeration module (28) are both electrically connected with the controller (23) for adjusting temperature to keep constant temperature, the temperature detection tube (24) penetrating through the diffusion plate (22) is arranged at the upper end of the controller (23).

2. The apparatus according to claim 1, wherein the apparatus is characterized by: The lower end of the controller (23) is provided with the connecting frame (29) fixedly connected with the mounting frame (1), the surface of the mounting frame (1) is provided with the cabinet door (4), the flow chamber (3) is fixedly connected with the mounting frame (5) at the edge line, and the cell sorting channel (17) is annularly arranged at the center of the upper surface of the mounting frame (5).

3. The integrated device for lung cancer cell sorting and detection of claim 2, wherein: The cell separation cavity (15) is arranged on the upper surface of the mounting frame (5) close to the corner, the fixed block (12) is arranged on the upper surface of the mounting frame (5) close to the center of the edge line, and the inlet (13) is fixedly connected to the center of the upper end of the fixed block (12).

4. The apparatus according to claim 3, wherein the apparatus is characterized by: The laser beam (14) is vertically arranged on the rear surface of the fixed block (12), and the inclined plate (10) is arranged inside the flow chamber (3) close to the edge line.

5. The integrated device for lung cancer cell sorting and detection of claim 2, wherein: The connecting channel (11) penetrating through the flow chamber (3) and the inclined plate (10) from left to right is arranged at the edge line of the upper surface of the mounting frame (5) away from the fixed block (12), and the separation module (16) is arranged on the upper surface of the mounting frame (5) at the upper end of the connecting channel (11).

6. The integrated device for lung cancer cell sorting and detection of claim 1, wherein: The detection module (9) corresponding to the connecting channel (11) is arranged on one side of the fixed frame (2) close to the flow chamber (3) from left to right, the display screen (18) is arranged at the center of the side of the fixed frame (2) away from the detection module (9), and the placing cabinet (7) is arranged between the fixed frame (2) and the mounting frame (1).

7. The integrated device for lung cancer cell sorting and detection of claim 6, wherein: The receiving plate (6) is arranged inside the placing cabinet (7), the pull plate (8) is arranged at the center of the surface of the receiving plate (6), and a plurality of groups of collection test tubes (19) are arranged inside the receiving plate (6) in sequence in the transverse direction.

8. The lung cancer cell sorting and detecting integrated device according to claim 7, wherein: The sliding blocks (20) are arranged on both sides of the placing cabinet (7), and the guide rails (21) are slidably connected to the outside of the sliding blocks (20) on both sides of the mounting frame (1).

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