Biological enzyme purification and separation device

Abstract

The utility model discloses a biological enzyme purification and separation device, and relates to the technical field of biological engineering. Under the action of the fixing mechanism, the shaking influence generated during purification and separation can be reduced, so that the actual use effect and quality can be ensured; the driver is started to drive the inner cylinder to perform circular motion, bio-enzyme liquid in the inner cylinder is purified and separated through the purification layer, and under the action of centrifugal force, the bio-enzyme liquid flows into the outer cylinder through the through holes in the inner cylinder and enters the crystallization equipment through the liquid discharge pipe and the connecting pipe for crystallization. The high-purity enzyme crystals can be obtained through crystallization of the crystallization equipment, the volume of the purified and filtered bio-enzyme liquid in the outer barrel can be monitored in real time through the liquid level sensor, and when the volume exceeds an early warning value, material injection into the hopper is stopped.

Description

Technical Field

[0001] This utility model relates to the field of bioengineering technology, specifically to a bioenzyme purification and separation device. Background Technology

[0002] Bioenzymes are organic substances secreted by living cells (such as bacteria, fungi, and actinomycetes) that can accelerate chemical reactions. In the preparation of bioenzymes, the enzymes extracted from the culture medium need to be purified by microfiltration to obtain higher purity enzymes. This is mainly achieved through microfiltration membranes.

[0003] Existing separation equipment is prone to non-fixed circular motion and swaying after high-speed operation, which affects the actual purification effect. To address this, we propose a bio-enzyme purification and separation device. Utility Model Content

[0004] The purpose of this invention is to provide a biological enzyme purification and separation device to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model specifically adopts the following technical solution:

[0006] A biological enzyme purification and separation device, comprising:

[0007] The outer cylinder has a detachable cover at its upper end, a hopper at the center of the cover, three drain pipes at its lower end, a connecting pipe at the output side of each drain pipe, and a crystallization device connected to the output side of the connecting pipe. Multiple liquid level sensors are detachably installed on the upper circumferential surface of the outer cylinder.

[0008] The outer walls on both sides of the outer cylinder are equipped with fixing mechanisms. Each fixing mechanism includes two support seats. A connecting column is detachably installed between the two support seats. A positioning column is installed on the outer side of each support seat. A positioning plate is detachably installed on the outer side of the positioning column.

[0009] The positioning plate and the crystallization device are provided with a base plate at their lower ends;

[0010] The purification and separation mechanism includes an inner cylinder disposed inside the outer cylinder, a drive motor disposed at the lower end of the inner cylinder, a purification layer disposed inside the inner cylinder, and a molecular sieve layer, an ion exchange layer and an affinity layer disposed sequentially from the inside to the outside of the purification layer, and a fixing frame detachably installed on the top of the inner cylinder.

[0011] Furthermore, the lower end face of the cylinder cover is provided with a groove that matches the outer diameter of the outer cylinder, and the upper end of the outer cylinder is installed in the groove.

[0012] Furthermore, a positioning block is installed on the lower circumferential outer wall of the hopper, a through hole is opened at the center of the cylinder cover, the hopper is inserted into the through hole, and a positioning groove is opened at the upper end of the through hole, and the positioning block is installed in the positioning groove.

[0013] Furthermore, a connecting block is installed on the outer side of the outer cylinder, and a connecting groove is opened on the inner side of the support seat, with the connecting block installed in the connecting groove.

[0014] Furthermore, both the support base and the positioning plate have positioning holes inside, and the two ends of the positioning column are inserted into the positioning holes.

[0015] Furthermore, each of the positioning plates is equipped with a snap-fit ​​block at its lower end, and the upper end of the base plate is provided with a snap-fit ​​groove, in which the snap-fit ​​block snaps into the snap-fit ​​groove. Additionally, the upper rear side of the base plate is provided with a groove, in which the lower end of the crystallization device sits.

[0016] Furthermore, the inner cylinder and the purification layer are provided with a plurality of discharge holes arranged in concentric circles, and a limit block is installed at the lower end of the inner cylinder. A circular settling groove is provided on the inner bottom wall of the inner cylinder, and the limit block is installed in the settling groove.

[0017] Furthermore, a drive gear is installed on the upper circumferential surface of the drive shaft of the drive machine, and a transmission tooth is provided at the center of the lower end of the inner cylinder, with the drive gear meshing with the transmission tooth.

[0018] Furthermore, the inner bottom wall of the inner cylinder is provided with a slot, the lower end of the purification layer is placed in the slot, and the inside of the fixing frame is provided with a limiting slot, and the upper ends of the inner cylinder and the purification layer are installed in the limiting slot.

[0019] The beneficial effects of this utility model are as follows:

[0020] 1. This utility model can achieve the installation stability of the positioning column under the action of the positioning plate, ensure the installation stability of the support seat under the action of the positioning column, ensure the installation stability of the two support seats under the action of the connecting column, and achieve the purpose of supporting the outer cylinder under the action of the support seat, thereby reducing the shaking effect generated during purification and separation, thus ensuring the actual use effect and quality.

[0021] 2. The starter motor of this utility model can drive the inner cylinder to perform circumferential motion. The bio-enzyme liquid inside the inner cylinder is purified and separated through the purification layer. Under the action of centrifugal force, the bio-enzyme liquid flows into the inner cylinder through the through hole inside the inner cylinder and enters the crystallization equipment through the drain pipe and connecting pipe for crystallization. High-purity enzyme crystals can be obtained through crystallization in the crystallization equipment. The volume of the purified and filtered bio-enzyme liquid inside the outer cylinder can be monitored in real time through the liquid level sensor. When the warning value is exceeded, the feeding into the hopper is stopped. Attached Figure Description

[0022] Figure 1 This is a three-dimensional schematic diagram of the present invention;

[0023] Figure 2 This is a front sectional view of the present invention;

[0024] Figure 3 This is a three-dimensional schematic diagram of the fixing mechanism in this utility model;

[0025] Figure 4 This is a three-dimensional schematic diagram of the purification and separation mechanism in this utility model;

[0026] Figure 5 This is an exploded perspective view of the outer cylinder of this utility model.

[0027] Reference numerals in the attached drawings: 1. Outer cylinder; 2. Fixing mechanism; 21. Support seat; 22. Connecting column; 23. Positioning column; 24. Positioning plate; 3. Purification and separation mechanism; 31. Inner cylinder; 32. Drive motor; 33. Purification layer; 331. Molecular sieve layer; 332. Ion exchange layer; 333. Affinity layer; 34. Fixing frame; 4. Cylinder cover; 5. Hopper; 6. Drain pipe; 7. Connecting pipe; 8. Base plate; 9. Liquid level sensor; 10. Crystallization equipment. Detailed Implementation

[0028] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings.

[0029] Please see Figure 1 - Figure 5 This utility model provides a biological enzyme purification and separation device, comprising:

[0030] The outer cylinder 1 has a detachable cover 4 at its upper end and a detachable hopper 5 at the center of the cover 4. The lower end of the outer cylinder 1 has three drain pipes 6, each drain pipe 6 has a connecting pipe 7 installed on its output side, and the output side of the connecting pipe 7 is connected to a crystallization device 10. Multiple liquid level sensors 9 are detachably installed on the upper circumferential surface of the outer cylinder 1.

[0031] The outer cylinder 1 can store the biological enzyme liquid. The cylinder cover 4 can ensure the sealing of the top of the outer cylinder 1 and ensure the installation stability of the hopper 5. The biological enzyme liquid can be injected into the inner cylinder 31 through the hopper 5. The biological enzyme liquid after purification and filtration inside the outer cylinder 1 enters the crystallization device 10 through the drain pipe 6 and the connecting pipe 7. After crystallization by the crystallization device 10, high-purity enzyme crystals can be obtained. The liquid level sensor 9 can monitor the volume of the purified and filtered biological enzyme liquid inside the outer cylinder 1 in real time. When the warning value is exceeded, the injection into the hopper 5 is stopped.

[0032] The outer walls on both sides of the outer cylinder 1 are equipped with fixing mechanisms 2. Each fixing mechanism 2 includes two support seats 21. A connecting column 22 is detachably installed between the two support seats 21. A positioning column 23 is installed on the outer side of each support seat 21. A positioning plate 24 is detachably installed on the outer side of the positioning column 23.

[0033] The positioning plate 24 ensures the installation stability of the positioning column 23, the positioning column 23 ensures the installation stability of the support seat 21, the connecting column 22 ensures the installation stability of the two support seats 21, and the support seat 21 supports the outer cylinder 1, reducing the shaking effect during purification and separation, thereby ensuring the actual use effect and quality.

[0034] A base plate 8 is provided at the lower end of the positioning plate 24 and the crystallization equipment 10.

[0035] The base plate 8 provides support for the positioning plate 24 and the crystallization equipment 10, thereby ensuring the stability of the installation and use of the positioning plate 24 and the crystallization equipment 10.

[0036] The purification and separation mechanism 3 includes an inner cylinder 31 disposed inside the outer cylinder 1. A drive motor 32 is disposed at the lower end of the inner cylinder 31. A purification layer 33 is disposed inside the inner cylinder 31. The purification layer 33 consists of a molecular sieve layer 331, an ion exchange layer 332, and an affinity layer 333 arranged sequentially from the inside to the outside. A fixing frame 34 is detachably installed on the upper part of the inner cylinder 31.

[0037] The inner cylinder 31 ensures the stability of the purification layer 33 during installation. Under the action of the drive mechanism 32, it provides support for the inner cylinder 31, while the purification layer 33 achieves its purification purpose. The fixing frame 34 ensures the stability of the upper part of the purification layer 33 during installation. The molecular sieve layer 331 improves overall drying efficiency and shortens the process cycle. The ion exchange layer 332 removes small molecule impurities, and the affinity layer 333 targets specific substrates or modified enzymes. Therefore, the purification effect is guaranteed under the action of the purification layer 33.

[0038] When the drive motor 32 is started, it can drive the inner cylinder 31 to perform circular motion. The bio-enzyme liquid inside the inner cylinder 31 is purified and separated through the purification layer 33. Under the action of centrifugal force, the bio-enzyme liquid flows into the inner cylinder 1 through the through hole inside the inner cylinder 31, and enters the crystallization device 10 through the drain pipe 6 and the connecting pipe 7 for crystallization.

[0039] In this embodiment, preferably, the lower end face of the cylinder cover 4 is provided with a groove that matches the outer diameter of the outer cylinder 1, and the upper end of the outer cylinder 1 is installed in the groove; under the action of the groove, the connection stability between the outer cylinder 1 and the cylinder cover 4 can be guaranteed, and the sealing purpose of the upper part of the outer cylinder 1 can be achieved.

[0040] In this embodiment, preferably, a positioning block is installed on the lower circumferential outer wall of the hopper 5, and a through hole is opened at the center of the cylinder cover 4. The hopper 5 is inserted into the through hole, and a positioning groove is opened at the upper end of the through hole. The positioning block is installed in the positioning groove. The through hole can achieve the purpose of installing the hopper 5, and the positioning groove can ensure the installation stability of the positioning block. The cooperation between the positioning block and the positioning groove can further ensure the installation stability of the hopper 5.

[0041] In this embodiment, preferably, a connecting block is installed on the outer side of the outer cylinder 1, and a connecting groove is opened on the inner side of the support 21, and the connecting block is installed in the connecting groove; the connecting groove can ensure the installation stability of the connecting block, and the connection stability between the outer cylinder 1 and the support 21 can be ensured by the cooperation of the connecting block and the connecting groove.

[0042] In this embodiment, preferably, both the support base 21 and the positioning plate 24 are provided with positioning holes, and the two ends of the positioning post 23 are inserted into the positioning holes; under the action of the positioning holes, the connection stability between the positioning post 23 and the support base 21 and the positioning plate 24 can be guaranteed, and it is convenient for assembly and installation.

[0043] In this embodiment, preferably, each positioning plate 24 has a snap-fit ​​block installed at its lower end, and the upper end of the base plate 8 has a snap-fit ​​groove, in which the snap-fit ​​block snaps into the snap-fit ​​groove. The upper rear side of the base plate 8 has a groove, in which the lower end of the crystallization device 10 sits. The snap-fit ​​groove ensures the stability of the snap-fit ​​installation, and the combination of the snap-fit ​​groove and the snap-fit ​​block ensures the stability of the connection between the positioning plate 24 and the base plate 8. The groove ensures the stability of the installation of the crystallization device 10.

[0044] In this embodiment, preferably, the inner cylinder 31 and the purification layer 33 are provided with a plurality of discharge holes arranged in concentric circles, and a limiting block is installed at the lower end of the inner cylinder 31. A circular settling groove is provided on the inner bottom wall of the inner cylinder 31, and the limiting block is installed in the settling groove. By realizing the discharge holes, the liquid inside the inner cylinder 31 can flow into the inner cylinder 1. Under the action of the settling groove, the limiting block can achieve the purpose of support. With the cooperation of the settling groove and the limiting block, the supporting purpose of the inner cylinder 31 can always be achieved.

[0045] In this embodiment, preferably, a drive gear is installed on the upper circumferential surface of the drive shaft of the drive machine 32, and a transmission tooth is provided at the lower center of the inner cylinder 31. The drive gear meshes with the transmission tooth; the stability of the transmission can be guaranteed by the cooperation of the drive gear and the transmission tooth.

[0046] In this embodiment, preferably, the inner bottom wall of the inner cylinder 31 is provided with a slot, the lower end of the purification layer 33 is placed in the slot, and the inside of the fixing frame 34 is provided with a limiting slot, and the upper ends of the inner cylinder 31 and the purification layer 33 are installed in the limiting slot; the slot can ensure the installation stability of the lower end of the purification layer 33, and the limiting slot can ensure the installation stability of the upper end of the inner cylinder 31 and the purification layer 33, that is, the installation stability of the purification layer 33 can be ensured by the cooperation of the fixing frame 34 and the slot.

[0047] Working principle and usage process of this utility model:

[0048] The outer cylinder 1 stores the bio-enzyme liquid, while the cap 4 ensures the airtightness of the top of the outer cylinder 1 and provides stability for the hopper 5. The hopper 5 allows bio-enzyme liquid to be injected into the inner cylinder 31. The purified and filtered bio-enzyme liquid inside the outer cylinder 1 enters the crystallization device 10 via the drain pipe 6 and connecting pipe 7, where it crystallizes to obtain high-purity enzyme crystals. The liquid level sensor 9 monitors the volume of the purified and filtered bio-enzyme liquid inside the outer cylinder 1 in real time; if the level exceeds the warning value, the injection into the hopper 5 is stopped. The positioning plate 24 ensures the stability of the positioning column 23, which in turn ensures the stability of the support 21. The connecting column 22 ensures the installation stability of the two support seats 21. The support seats 21 support the outer cylinder 1, reducing the shaking effect during purification and separation, thus ensuring the actual use effect and quality. The base plate 8 supports the positioning plate 24 and the crystallization device 10, thus ensuring the installation and use stability of the positioning plate 24 and the crystallization device 10. Starting the drive motor 32 can drive the inner cylinder 31 to perform circumferential motion. The bio-enzyme liquid inside the inner cylinder 31 is purified and separated through the purification layer 33. Under the action of centrifugal force, the bio-enzyme liquid flows into the inner cylinder 1 through the through hole inside the inner cylinder 31, and enters the crystallization device 10 through the drain pipe 6 and the connecting pipe 7 for crystallization.

[0049] The above description of the disclosed embodiments enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A bio-enzyme purification and separation device, characterized in that, Include: The upper end of the outer cylinder (1) is detachably mounted with a cylinder cover (4), the center of the cylinder cover (4) is detachably mounted with a hopper (5), the lower end of the outer cylinder (1) is detachably mounted with three drain pipes (6), the output side of each drain pipe (6) is mounted with a connecting pipe (7), the output side of the connecting pipe (7) is connected with a crystallization equipment (10), the circumferential surface of the upper part of the outer cylinder (1) is detachably mounted with a plurality of liquid level sensors (9); The outer wall of both sides of the outer cylinder (1) is mounted with a fixing mechanism (2), each fixing mechanism (2) comprises two supporting seats (21), two supporting seats (21) are detachably mounted with a connecting column (22), the outer side of each supporting seat (21) is mounted with a positioning column (23), the outer side of the positioning column (23) is detachably mounted with a positioning plate (24); The lower end of the positioning plate (24) and the crystallization equipment (10) is provided with a bottom plate (8); The purification separation mechanism (3) comprises an inner cylinder (31) arranged in the outer cylinder (1), a driving machine (32) arranged at the lower end of the inner cylinder (31), a purification layer (33) arranged in the inner cylinder (31), the purification layer (33) is sequentially provided with a molecular sieve layer (331), an ion exchange layer (332) and an affinity layer (333) from inside to outside, and a fixing frame (34) detachably mounted above the inner cylinder (31).

2. The device for purifying and separating biological enzymes according to claim 1, characterized in that: The lower end surface of the cylinder cover (4) is provided with a sink groove consistent with the outer diameter of the outer cylinder (1), and the upper end of the outer cylinder (1) is mounted in the sink groove.

3. The device of claim 1, wherein: The circumferential outer wall of the lower part of the hopper (5) is provided with a positioning block, the center of the cylinder cover (4) is provided with a through hole, the hopper (5) is inserted into the through hole, and the upper end of the through hole is provided with a positioning groove, and the positioning block is mounted in the positioning groove.

4. The device of claim 1, wherein: The outer side of the outer cylinder (1) is provided with a connecting block, and the inner side of the supporting seat (21) is provided with a connecting groove, and the connecting block is mounted in the connecting groove.

5. The device of claim 1, wherein: The inner sides of the supporting seat (21) and the positioning plate (24) are provided with positioning holes, and the two ends of the positioning column (23) are inserted into the positioning holes.

6. The device of claim 1, wherein: The lower end of each positioning plate (24) is mounted with a clamping block, the upper end of the bottom plate (8) is provided with a clamping groove, the clamping block is clamped in the clamping groove, and the upper end of the rear side of the bottom plate (8) is provided with a groove, and the lower end of the crystallization equipment (10) is seated in the groove.

7. The device of claim 1, wherein: The inner cylinder (31) and the purification layer (33) are provided with a plurality of discharge holes arranged in concentric circles, and the lower end of the inner cylinder (31) is provided with a limiting block, and the inner bottom wall of the inner cylinder (31) is provided with a circular sink groove, and the limiting block is mounted in the sink groove.

8. The device of claim 1, wherein: The driving shaft of the driving machine (32) is provided with a driving gear on the circumferential surface of the upper end, the lower center of the inner cylinder (31) is provided with a transmission gear, and the driving gear is connected with the transmission gear.

9. The device of claim 1, wherein: The inner bottom wall of the inner cylinder (31) is provided with a hollow groove, the lower end of the purification layer (33) is arranged in the hollow groove, and the inner part of the fixing frame (34) is provided with a limiting groove, and the inner cylinder (31) and the upper end of the purification layer (33) are installed in the limiting groove.

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