Sterile sampling device of fermentation tank

Abstract

The utility model discloses a sterile sampling device for a fermentation tank, which is provided with a sampling mechanism, before the sterile sampling device is used, the interior is sterilized by high-temperature sterilization steam, then the sterile sampling device is flushed by alcohol, an infusion pump is started to be matched with an infusion pipe to extract fermentation liquid in the tank body, and the fermentation liquid enters a sampling pipe; after sampling is finished, a third valve is closed immediately, the sampling bottle is separated from the liquid inlet pipe and the second connecting pipe above the sampling bottle, at the moment, residual liquid exists in the sampling pipe, and a fifth valve is opened again, so that the residual liquid in the sampling bottle is separated from the second connecting pipe; after the alcohol in the liquid storage box is pushed into the sampling pipe again, after the interior of the sampling pipe, the front end of the connecting pipe and the interior of the second connecting pipe are flushed through the alcohol, a second valve above a liquid discharging pipe below is opened to discharge the alcohol in the sampling pipe, and meanwhile residual liquid in the sampling pipe is flushed out.

Description

Technical Field

[0001] This utility model relates to the technical field of aseptic sampling devices for fermenters, specifically an aseptic sampling device for fermenters. Background Technology

[0002] Currently, in the process of microbial fermentation culture, it is necessary to perform aseptic sampling of the fermentation broth for contamination detection and shake-flask experiments. This requires ensuring that the entire sampling process is free from contamination. Traditional aseptic sampling involves disinfecting the environment and then collecting samples in sterile test tubes or Erlenmeyer flasks under flowing fermentation broth conditions. However, existing devices have some shortcomings; for example...

[0003] For example, a fermenter aseptic sampling device, as disclosed in announcement number CN217516940U, includes a fermenter and a sampling device. The fermenter has a first connecting pipe, which is L-shaped and penetrates the side wall of the fermenter. A water pump is mounted on the first connecting pipe. The sampling device has a cavity, and a third connecting pipe penetrates the side wall of the sampling device, communicating with the cavity. The first and third connecting pipes are connected via a second connecting pipe. This device has some problems. While it can be used effectively to perform aseptic sampling from the fermenter, it typically only allows for internal sterilization before sampling. Because it is inconvenient to clean and sterilize the pipes after sampling, we propose a fermenter aseptic sampling device to address these issues. Utility Model Content

[0004] The purpose of this invention is to provide a sterile sampling device for fermenters, so as to solve the problem mentioned in the background art that it is inconvenient to clean and sterilize the pipeline after sampling when using the sterile sampling device for fermenters.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a sterile sampling device for a fermenter, comprising a tank body and a sampling bottle;

[0006] The tank is equipped with a liquid extraction pipe inside, and a liquid extraction pump is installed above the liquid extraction pipe. The liquid extraction pipe and the sampling pipe are threaded together. The sampling pipe is connected to an air inlet pipe above, and a first valve is installed above the air inlet pipe. The sampling pipe is connected to a first connecting pipe above, and one end of the first connecting pipe is connected to a liquid storage box. A fifth valve is installed above the first connecting pipe. The liquid storage box is equipped with a liquid injection mechanism inside.

[0007] As a preferred technical solution of this utility model, the liquid injection mechanism includes a liquid storage box, a push rod, and a push plate. The upper part of the liquid storage box is slidably connected to the push rod, and the front end of the push rod is fixedly connected to the push plate. A rubber gasket is provided on the surface of the push plate.

[0008] As a preferred embodiment of this utility model, an injection pipe is provided above the liquid storage box, and a fourth valve is provided above the injection pipe.

[0009] As a preferred embodiment of this utility model, a drain pipe is provided below the sampling tube, and a second valve is provided above the drain pipe.

[0010] As a preferred technical solution of this utility model, the liquid extraction tube, sampling tube, first connecting tube and second connecting tube are pipes made of high temperature resistant and corrosion resistant materials.

[0011] As a preferred embodiment of this utility model, the sampling tube is connected to the second connecting tube, and the second connecting tube is threadedly connected to the liquid inlet tube above the sampling bottle, and a third valve is provided above the liquid inlet tube.

[0012] Compared with the prior art, the beneficial effects of this utility model are as follows: The aseptic sampling device for the fermenter is equipped with a sampling mechanism. Before use, the inside is sterilized by high-temperature sterilization steam and then rinsed with alcohol. The pump is started and the liquid is pumped out of the fermentation liquid inside the tank through the pumping pipe and enters the sampling tube. It then flows downward and enters the second connecting pipe and enters the pre-sterilized sealed sampling bottle to complete the sampling. After the sampling is completed, the third valve is immediately closed to separate the sampling bottle from the upper inlet pipe and the second connecting pipe. At this time, there will be residual liquid inside the sampling tube. The fifth valve is opened again to push the alcohol in the storage box into the sampling tube again. The inside of the sampling tube, the front end of the connecting pipe and the inside of the second connecting pipe are rinsed with alcohol. Then, the second valve above the lower drain pipe is opened to drain the alcohol inside and flush out the residual liquid inside. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the main cross-section of the present invention;

[0014] Figure 2 For the present utility model Figure 1 Enlarged structural diagram at point A in the middle;

[0015] Figure 3 This is a schematic diagram of the liquid storage box structure of this utility model.

[0016] In the diagram: 1. Tank; 2. Suction pipe; 3. Suction pump; 4. Sampling pipe; 5. Air inlet pipe; 6. First valve; 7. Drain pipe; 8. Second valve; 9. First connecting pipe; 10. Fifth valve; 11. Storage box; 12. Push rod; 13. Push plate; 14. Second connecting pipe; 15. Third valve; 16. Sampling bottle; 17. Injection pipe; 18. Fourth valve; 19. Inlet pipe. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] Please see Figure 1-3 This utility model provides a technical solution: a sterile sampling device for a fermenter, comprising a tank body 1 and a sampling bottle 16. A suction pipe 2 is installed inside the tank body 1, and a suction pump 3 is installed above the suction pipe 2. The suction pipe 2 and a sampling pipe 4 are threadedly connected. A drain pipe 7 is installed below the sampling pipe 4, and a second valve 8 is installed above the drain pipe 7. The sampling pipe 4 is connected to an air inlet pipe 5, and a first valve 6 is installed above the air inlet pipe 5. The device includes a suction pipe 2, a sampling pipe 4, a first connecting pipe 9, and a second connecting pipe 16. 4 is a pipe made of high temperature and corrosion resistant material. The sampling pipe 4 is connected to the second connecting pipe 14, and the second connecting pipe 14 is threadedly connected to the liquid inlet pipe 19 above the sampling bottle 16. A third valve 15 is provided above the liquid inlet pipe 19. The sampling pipe 4 is connected to the first connecting pipe 9 above, and one end of the first connecting pipe 9 is connected to the liquid storage box 11. A fifth valve 10 is provided above the first connecting pipe 9. A liquid injection pipe 17 is provided above the liquid storage box 11, and a fourth valve 18 is provided above the liquid injection pipe 17.

[0019] In use, after connecting the sampling tube 4 and the liquid extraction tube 2 above the tank 1 with threads, connect the second connecting tube 14 below the sampling tube 4 to the liquid inlet tube 19 above the sampling bottle 16 with threads. Then connect the air inlet tube 5 above the sampling tube 4 to the external high-temperature sterilization steam. Open the first valve 6 above the air inlet tube 5 to allow the high-temperature sterilization steam to enter the sampling tube 4, the first connecting tube 9, the exposed part of the connecting tube, and the interior of the second connecting tube 14 for sterilization. Simultaneously, open the second valve 8 above the lower drain tube 7 to discharge excess steam. Then close the first valve 6 above the air inlet tube 5, the fifth valve 10 above the first connecting tube 9, and the second valve 8 above the lower drain tube 7. Finally, open the third valve 15 above the liquid inlet tube 19. After the fermentation liquid inside the tank 1 is extracted by the pump 3 and the pump pipe 2, it enters the sampling pipe 4 and flows downward into the second connecting pipe 14. It then enters the pre-sterilized sealed sampling bottle 16 to complete the sampling. After the sampling is completed, the third valve 15 above is closed to separate the sampling bottle 16 from the inlet pipe 19 and the second connecting pipe 14. At this time, there will be residual liquid inside the sampling pipe 4. The fifth valve 10 above the first connecting pipe 9 is opened again to push the alcohol in the storage box 11 into the sampling pipe 4. The alcohol is used to rinse the inside of the sampling pipe 4, the front end of the pump pipe 2, and the inside of the second connecting pipe 14. Then, the second valve 8 above the drain pipe 7 below is opened to drain the alcohol and rinse out the residual liquid.

[0020] The liquid storage box 11 is equipped with a liquid injection mechanism, which includes the liquid storage box 11, the push rod 12, and the push plate 13. The upper part of the liquid storage box 11 is slidably connected to the push rod 12, and the front end of the push rod 12 is fixedly connected to the push plate 13. A rubber gasket is provided on the surface of the push plate 13.

[0021] At this time, the fifth valve 10 above the first connecting tube 9 can be opened, and the push rod 12 above the liquid storage box 11 can be pushed, so that the push rod 12 drives the push plate 13 to slide inside the liquid storage box 11. At this time, the push plate 13 pushes the sterilizing alcohol inside into the first connecting tube 9 and then into the sampling tube 4. The inside is rinsed with alcohol, and the alcohol produced by rinsing is discharged through the drain pipe 7 below. When the alcohol inside the liquid storage box 11 is used up, the injection pipe 17 above the liquid storage box 11 can be connected to the external alcohol, and the fourth valve 18 above can be opened. Then, the push rod 12 is pulled to drive the push plate 13 to move, and the push plate 13 draws the external alcohol into the inside to replenish it.

[0022] Working principle: When using the aseptic sampling device for the fermenter, connect the sampling tube 4 and the liquid extraction tube 2 above the tank 1 via threads. Then, connect the second connecting tube 14 below the sampling tube 4 to the liquid inlet tube 19 above the sampling bottle 16 via threads. At this time, connect the air inlet tube 5 above the sampling tube 4 to the external high-temperature sterilization steam. Open the first valve 6 above the air inlet tube 5 to allow the high-temperature sterilization steam to enter the sampling tube 4, the first connecting tube 9, the exposed part of the connecting tube, and the interior of the second connecting tube 14. Sterilization is performed by the high-temperature sterilization steam. At the same time, the second valve 6 above the drain tube 7 below can be opened. Valve 8 discharges excess steam. At this point, the fifth valve 10 above the first connecting pipe 9 can be opened, pushing the push rod 12 above the liquid storage box 11. This causes the push rod 12 to slide the push plate 13 inside the liquid storage box 11. The push plate 13 then pushes the sterilizing alcohol into the first connecting pipe 9 and into the sampling tube 4, rinsing the interior with alcohol. The resulting alcohol is discharged through the lower drain pipe 7. At this point, the first valve 6 above the air inlet pipe 5, the fifth valve 10 above the first connecting pipe 9, and the second valve 8 above the lower drain pipe 7 are closed. Then, the third valve 10 above the liquid inlet pipe 19 is opened. 5. Open the pump. At this time, start the pump 3 and pump 2 to extract the fermentation liquid from inside tank 1 and into sampling tube 4. The liquid then flows downward and into second connecting tube 14, and finally into pre-sterilized sealed sampling bottle 16 to complete the sampling. Immediately after sampling, close the third valve 15 to separate sampling bottle 16 from inlet tube 19 and second connecting tube 14. At this time, there will be residual liquid inside sampling tube 4. Open the fifth valve 10 above first connecting tube 9 again to push the alcohol in storage box 11 into sampling tube 4. The alcohol will then be used to sample the liquid. After rinsing the inside of tube 4, the front end of the suction tube 2, and the inside of the second connecting tube 14, open the second valve 8 above the lower drain tube 7 to drain the alcohol inside and flush out the residual liquid inside. When the alcohol inside the storage box 11 is used up, connect the injection tube 17 above the storage box 11 to the external alcohol, open the fourth valve 18 above, pull the push rod 12 to move the push plate 13, and the push plate 13 draws the external alcohol into the inside to replenish it, thus completing a series of operations. The contents not described in detail in this specification are existing technologies known to those skilled in the art.

[0023] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A sterile sampling device for a fermenter, comprising a tank body (1) and a sampling bottle (16); Its features are: The tank (1) is equipped with a liquid extraction pipe (2) inside, and a liquid extraction pump (3) is installed above the liquid extraction pipe (2). The liquid extraction pipe (2) and the sampling pipe (4) are threaded together. The sampling pipe (4) is connected to the air inlet pipe (5) above, and a first valve (6) is installed above the air inlet pipe (5). The sampling pipe (4) is connected to the first connecting pipe (9) above, and one end of the first connecting pipe (9) is connected to the liquid storage box (11). A fifth valve (10) is installed above the first connecting pipe (9). The liquid storage box (11) is equipped with a liquid injection mechanism inside.

2. The aseptic sampling device for a fermenter according to claim 1, characterized in that, The liquid injection mechanism includes a liquid storage box (11), a push rod (12), and a push plate (13). The upper part of the liquid storage box (11) is slidably connected to the push rod (12), and the front end of the push rod (12) is fixedly connected to the push plate (13). A rubber gasket is provided on the surface of the push plate (13).

3. The aseptic sampling device for a fermenter according to claim 1, characterized in that, A liquid injection pipe (17) is provided above the liquid storage box (11), and a fourth valve (18) is provided above the liquid injection pipe (17).

4. The aseptic sampling device for a fermenter according to claim 1, characterized in that, A drain pipe (7) is provided below the sampling tube (4), and a second valve (8) is provided above the drain pipe (7).

5. The aseptic sampling device for a fermenter according to claim 1, characterized in that, The liquid extraction tube (2), sampling tube (4), first connecting tube (9) and second connecting tube (14) are pipes made of high temperature and corrosion resistant materials.

6. The aseptic sampling device for a fermenter according to claim 1, characterized in that, The sampling tube (4) is connected to the second connecting tube (14), and the second connecting tube (14) is threadedly connected to the liquid inlet tube (19) above the sampling bottle (16), and a third valve (15) is provided above the liquid inlet tube (19).

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